Full-automatic sample adding fluorescent quantitative PCR (Polymerase Chain Reaction) instrument based on biological test

Abstract

The invention discloses a full-automatic sample adding fluorescent quantitative PCR instrument based on biological testing, and relates to the technical field of biomedical engineering.The full-automatic sample adding fluorescent quantitative PCR instrument comprises an analysis mechanism, a processing mechanism is arranged in a shell and comprises a connecting block, and two processing tanks are arranged on the upper side of the connecting block; a flow guide shell and a connecting shell are additionally arranged on the upper side of the connecting block, a fan is additionally arranged between the interior of the flow guide shell and the interior of the connecting shell, the remaining two air inlet ends of a communicating pipe communicate with first electric valves, and two air outlets in the top of the other end of the connecting block communicate with second electric valves; by arranging the treatment mechanism, key areas in the instrument can be comprehensively covered and disinfected, meanwhile, disinfection gas left in the instrument can be treated, the disinfection effect is good, and the disinfection effect is good. Therefore, the use efficiency of the full-automatic sample adding fluorescent quantitative PCR instrument based on the biological test is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biomedical engineering, in particular to a full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing. BACKGROUND

[0002] The full-automatic sample adding fluorescence quantitative PCR instrument belongs to a microorganism detection and analysis instrument, and its core function is to rapidly and accurately detect microorganisms (bacteria, viruses, fungi, etc.) or other biological targets.

[0003] The existing full-automatic sample adding fluorescence quantitative PCR instrument still has the following deficiencies in actual use: When the full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing is in use, the self-provided disinfection lamp has obvious limitations and can only perform local disinfection, that is, only the objects placed inside the storage bin are disinfected, and the key areas such as the sample holder, the reaction chamber and the needle washing groove of the needle washer inside the instrument cannot be fully covered and disinfected, and the instrument also lacks the function of processing the disinfection gas remaining inside, that is, the disinfection and subsequent residual gas processing of the instrument all depend on manual operation, thereby increasing the working intensity of the operator.

[0004] Therefore, we propose a new full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing to solve the problems raised in the above background. SUMMARY

[0005] The purpose of the present application is to provide a full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing, which can fully cover and disinfect the key areas inside the instrument by setting a processing mechanism, and can also process the disinfection gas remaining inside the instrument, so as to solve the problems raised in the above background.

[0006] To achieve the above purpose, the present application provides the following technical scheme: a full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing, comprising an analysis mechanism, the analysis mechanism comprising a base and a shell, a processing mechanism is arranged inside the shell, and the processing mechanism is used for disinfecting all components inside the shell and processing the residual disinfection gas. The processing mechanism comprises a connecting block, a top opening of one end of the connecting block is provided with a top cover, an air inlet end of the top cover is communicated with a one-way valve, an upper side of the connecting block is provided with two processing tanks, an upper side of the connecting block is provided with a flow guide shell and a connecting shell, a fan is arranged between the flow guide shell and the connecting shell, an air inlet end of the connecting shell is communicated with a communication pipe, remaining two air inlet ends of the communication pipe are communicated with first electric valves, an air inlet end of one of the first electric valves is communicated with a conveying pipe, air inlet ends of the two processing tanks are communicated with check valves, air inlet ends of the two check valves are communicated with first connecting pipes, an air outlet end of the flow guide shell is bonded with a sealing ring, two air outlet openings of the other end of the connecting block are communicated with second electric valves, air outlet ends of the two second electric valves are respectively communicated with a three-way pipe and an exhaust pipe, remaining two ports of the three-way pipe are respectively communicated with a third electric valve and an auxiliary ring, an air inlet end of the other first electric valve is communicated with a processing box, a top of the processing box is provided with a box cover, an air inlet end of the box cover is communicated with a second connecting pipe.

[0007] Preferably, a plurality of first adsorption blocks and a plurality of perforated partitions are arranged inside the top opening of one end of the connecting block, the plurality of first adsorption blocks and the plurality of perforated partitions are stacked with each other, an air inlet end of the one-way valve is communicated with a circular pipe, and an air inlet end of the circular pipe is communicated with an arc-shaped pipe.

[0008] Preferably, an air outlet end of the arc-shaped pipe is fixedly penetrated through an outer wall of the shell, the connecting block is fixedly arranged in the shell, a fixing block is fixedly arranged between the two processing tanks, the fixing block is arranged on the upper side of the connecting block, and an air outlet end of the top opening of one end of the connecting block is communicated with an air inlet end of the conveying pipe.

[0009] Preferably, an air inlet end of one of the first connecting pipes is communicated with an air outlet end of one of the processing tanks, a surface of the sealing ring is abutted with a surface of the other end of the connecting block corresponding to the air inlet opening, and an air outlet end of the third electric valve is communicated with an air inlet end of the other first connecting pipe.

[0010] Preferably, an air outlet end of the exhaust pipe is fixedly penetrated through an inner wall of the shell, the auxiliary ring is arranged on the inner wall of the shell, a second adsorption block is arranged in the processing box, an air inlet end of the second connecting pipe is communicated with an air outlet end of the other processing tank, and the processing box is arranged on an outer wall of the connecting shell.

[0011] Preferably, the base is arranged on a bottom end of the shell, a cover plate is rotatably connected to a top opening of the shell, a mechanical arm is arranged on an inner wall of the base, and a suction head loading and unloading device is arranged on a front end of the mechanical arm.

[0012] Preferably, the inner wall bottom of the base is additionally provided with a control box, a sample holder, a temperature control oscillator, a needle washer, a thermal cycler and a power module, the thermal cycler is additionally provided with a fluorescence detector, and a plurality of hexagonal columns are fixed to the inner wall bottom of the base, and a control panel is additionally provided between the top portions of the hexagonal columns.

[0013] Preferably, the side square hole of the shell is movably sleeved with a collection bin, the top of the collection bin is additionally provided with a bin cover, the inner wall bottom of the base is fixed with a mounting bracket, the mounting bracket is additionally provided with an electromagnet, the magnetic attraction end of the electromagnet is magnetically connected with the outer wall of the collection bin, the inside of the collection bin is provided with a plurality of disinfection lamps, and the bottom of the bin cover is additionally provided with the plurality of disinfection lamps.

[0014] Preferably, the inside of the shell is additionally provided with a display module, the display end of the display module movably penetrates the inner wall of the shell, the top of the side flat plate of the shell is additionally provided with an alarm, the inner wall of the shell is additionally provided with a switch, the control end of the switch movably penetrates the inner wall of the shell, and the inside of the shell is additionally provided with an auxiliary rod.

[0015] Preferably, the outer wall heat dissipation hole of the shell is fixed with an outer shell, the inside of the outer shell is placed with a baffle and a sealing gasket, the surface of the sealing gasket is in contact with the outer wall of the shell, the surface of the connecting block is fixed with a plurality of support plates, the bottom of each support plate is in contact with the inner wall bottom of the base, the upper side of the connecting block is additionally provided with a fixing bracket, the fixing bracket is used for stabilizing the working of the conveying pipe, and the top end of the control box is fixed with an elastic rope.

[0016] Compared with the prior art, the present application has the following beneficial effects: 1. In the present application, the treatment mechanism is provided, which can comprehensively disinfect the key areas inside the instrument, and can also treat the disinfectant gas remaining in the instrument, thereby improving the use efficiency of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological testing. When the detection sample completes the biological testing operation, the disinfectant gas conveyed by the pipe is first dried and then conveyed into the flow guide shell through the cooperation of the fan, the first adsorption block and the corresponding accessories, and then the dried disinfectant gas is uniformly dispersed in the space composed of the shell and the base through the cooperation of the fan, the auxiliary ring and the corresponding accessories, thereby comprehensively disinfecting the key areas inside the instrument.

[0017] 2. In the present application, the disinfectant gas remaining in the instrument is sucked and then reacted and removed through the cooperation of the fan, the treatment tank and the corresponding accessories, and then the gas obtained by the reaction is dried to prevent moisture from entering the inside of the flow guide shell and the connecting shell, and then the dried gas obtained by the reaction is discharged into the environment through the cooperation of the exhaust pipe.

[0018] 3. In the present application, by setting the analysis mechanism, the detection sample can be automatically tested. When the biological test of the detection sample is needed, the PCR premix solution in the reagent tube on the sample holder and the detection sample solution in the sample tube can be sequentially injected into the PCR reaction tube on the temperature control oscillator through the display module, the control panel, the mechanical arm, the suction head mounting and dismounting device, the control box and the two equipped hoses. At the same time, the used sample adding suction head can be sent into the collection bin for collection. At the same time, the tube on the sample holder can be blocked at the tube opening of the PCR reaction tube. Then, the two liquids in the PCR reaction tube can be fully mixed through the cooperation of the temperature control oscillator. Then, the biological test result of the detection sample can be obtained through the cooperation of the thermal cycler and the fluorescence detector, the control panel, the pre-stored positive and negative control samples and the display module. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a side view angle perspective view of the cover plate of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application when closed. Figure 2 It is a side view angle perspective view of the cover plate of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application when opened. Figure 3 It is a top view angle partial structure schematic view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 4 It is a side view angle partial structure schematic view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 5 It is a top view angle partial structure schematic view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 6 It is a side view angle partial structure schematic view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 7 It is another angle partial cross-sectional perspective view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 8 It is a processing mechanism partial cross-sectional perspective view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 9 It is a processing mechanism partial cross-sectional perspective view of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 5 It is a structure enlarged perspective view of A in the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 10 It is a perspective structure schematic view of the shell, baffle and sealing gasket of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test of the present application. Figure 11 Figure 1 is a schematic diagram of the analysis mechanism of the full-automatic sample adding fluorescence quantitative PCR instrument based on biological test according to the present application.

[0020] In the figure: 1, analysis mechanism; 101, base; 102, shell; 103, cover plate; 104, mechanical arm; 105, suction head loading and unloading device; 106, control box; 107, sample rack; 108, temperature control oscillator; 109, needle washer; 110, thermal cycler; 111, fluorescence detector; 112, power module; 113, hexagonal column; 114, control panel; 115, collection bin; 116, bin cover; 117, mounting rack; 118, electromagnet; 119, disinfection lamp; 120, display module; 121, alarm; 122, switch; 123, auxiliary rod; 2, outer shell; 3, baffle; 4, processing mechanism; 401, connecting block; 402, first adsorption block; 403, perforated partition; 404, top cover; 405, one-way valve; 406, round pipe; 407, arc pipe; 408, processing tank; 409, fixing block; 410, flow guide shell; 411, fan; 412, connecting shell; 413, communication pipe; 414, first electric valve; 415, conveying pipe; 416, check valve; 417, first connecting pipe; 418, sealing ring; 419, second electric valve; 420, tee pipe; 421, third electric valve; 422, exhaust pipe; 423, auxiliary ring; 424, processing box; 425, second adsorption block; 426, box cover; 427, second connecting pipe; 5, fixing frame; 6, support plate; 7, elastic cord; 8, sealing gasket. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0022] Embodiment one: please refer to Figures 1-5 and Figures 9-11As shown, the present application provides a technical solution: a full-automatic sample adding fluorescence quantitative PCR instrument based on biological test, comprising an analysis mechanism 1, the analysis mechanism 1 comprising a base 101 and a shell 102, the base 101 is installed with the bottom end of the shell 102, the top opening of the shell 102 is rotatably connected with a cover plate 103, the inner wall bottom of the base 101 is additionally provided with a mechanical arm 104, the front end of the mechanical arm 104 is additionally provided with a suction head loading and unloading device 105, the inner wall bottom of the base 101 is additionally provided with a control box 106, a sample rack 107, a temperature control oscillator 108, a needle washer 109, a thermal cycler 110 and a power module 112, the thermal cycler 110 is additionally provided with a fluorescence detector 111, the inner wall bottom of the base 101 is fixed with a plurality of hexagonal columns 113, the top of the plurality of hexagonal columns 113 is additionally provided with a control panel 114, the inside of the side square hole of the shell 102 is movably sleeved with a collection bin 115, the top of the collection bin 115 is additionally provided with a bin cover 116, the inner wall bottom of the base 101 is fixed with a mounting bracket 117, the mounting bracket 117 is additionally provided with an electromagnet 118, the magnetic suction end of the electromagnet 118 is magnetically connected with the outer wall of the collection bin 115, the inside of the collection bin 115 is provided with a plurality of disinfection lamps 119, the plurality of disinfection lamps 119 are additionally provided at the bottom of the bin cover 116, the inside of the shell 102 is additionally provided with a display module 120, the display end of the display module 120 is movably penetrated through the inner wall of the shell 102, the top of the side flat plate of the shell 102 is additionally provided with an alarm 121, the inner wall of the shell 102 is additionally provided with a switch 122, the control end of the switch 122 is movably penetrated through the inner wall of the shell 102, the inside of the shell 102 is additionally provided with an auxiliary rod 123, the top end of the control box 106 is fixed with a elastic rope 7, the outer wall of the shell 102 is fixed with an outer shell 2 at the heat dissipation hole, the inside of the outer shell 2 is placed with a baffle 3 and a sealing gasket 8, the surface of the sealing gasket 8 is in contact with the outer wall of the shell 102.

[0023] When the biological sample needs to be detected, the operator can directly press the start button on the display module 120, and the display module 120 will cooperate with the control panel 114 to start the mechanical arm 104, the needle cleaner 109 and all the disinfection lamps 119. Then the front end of the mechanical arm 104 drives the suction head mounting and dismounting device 105 to move. When the suction head end of the suction head mounting and dismounting device 105 moves to the sample rack 107 and is connected to the sample suction head, the control box 106 and one of the hoses cooperate to fix the sample suction head. Then the sample suction head is moved to the needle cleaning groove of the needle cleaner 109 for cleaning. After cleaning, the sample suction head is moved to the reagent tube for sucking the quantitative PCR premix solution. Then the sample suction head is moved to the PCR reaction tube of the temperature-controlled oscillator 108. When the sample suction head completes the position movement, the PCR premix solution in the sample suction head is injected into the PCR reaction tube. Then the sample suction head is moved to the square hole position of the warehouse cover 116, and the sample suction head is released from the fixing of the suction head mounting and dismounting device 105, so that the sample suction head falls into the collection warehouse 115. After the above operation steps are repeated, the front end of the suction head mounting and dismounting device 105 fixes a new sample suction head. Then the new sample suction head is moved to the needle cleaning groove of the needle cleaner 109 for cleaning. After cleaning, the sample suction head is moved to the sample tube for sucking the quantitative detection sample solution. Then the detection sample solution is injected into the PCR reaction tube containing the PCR premix solution, and the two solutions are mixed together. When the detection sample solution completes the solution injection, the sample suction head is released and sent into the collection warehouse 115. Then the suction head mounting and dismounting device 105 fixes the tube plug, and then moves the tube plug to the tube opening of the PCR reaction tube to block it. After the blocking operation is completed, the tube plug is released, and then the mechanical arm 104 is closed. The temperature-controlled oscillator 108 is started, which fully mixes the two solutions in the PCR reaction tube. When the two solutions complete the mixing operation, the temperature-controlled oscillator 108 is closed. Then the mechanical arm 104, the suction head mounting and dismounting device 105, the fixed relationship between the tube plug and the PCR reaction tube, the control box 106 and the corresponding two hoses cooperate to move the PCR reaction tube containing the mixed solution to the placement plate of the thermal cycler 110. Then the tube plug is released, and the suction head mounting and dismounting device 105 is reset to the original position. Then the thermal cycler 110 is started to control the reaction temperature of the mixed solution in the PCR reaction tube. Then the fluorescence detector 111 is started, which emits excitation light to the PCR reaction tube, collects the reflected fluorescent signal, converts the collected fluorescent signal into a digital signal, and transmits it to the control panel 114.After receiving the data, the control board 114 processes the data, draws a fluorescence amplification curve, calculates the Ct value, compares the Ct value with the data of the positive and negative control samples stored in advance, obtains the test result of the detection sample, and transmits the test result to the display module 120 and displays the test result on the screen of the display module 120. At the same time, the test result is stored in the storage module integrated on the control board 114 for later viewing. In this way, the detection sample can be automatically tested.

[0024] Embodiment two: according to Figures 1-10As shown, the analysis mechanism 1 comprises a base 101 and a shell 102, the inside of the shell 102 is provided with a processing mechanism 4 for sterilizing all components inside the shell 102 and processing residual sterilizing gas, the processing mechanism 4 comprises a connecting block 401, one end of the connecting block 401 is provided with a top opening, and a top cover 404 is additionally arranged at the top opening, the gas inlet end of the top cover 404 is communicated with a one-way valve 405, the upper side of the connecting block 401 is provided with two processing tanks 408, the upper side of the connecting block 401 is additionally provided with a flow guide shell 410 and a connecting shell 412, a fan 411 is additionally arranged between the inside of the flow guide shell 410 and the inside of the connecting shell 412, the gas inlet end of the connecting shell 412 is communicated with a communication pipe 413, the remaining two gas inlet ends of the communication pipe 413 are both communicated with a first electric valve 414, the gas inlet end of one of the first electric valves 414 is communicated with a conveying pipe 415, the gas inlet ends of the two processing tanks 408 are both communicated with a check valve 416, the gas inlet ends of the two check valves 416 are both communicated with a first connecting pipe 417, the gas outlet end surface of the flow guide shell 410 is bonded with a sealing ring 418, the two gas outlet openings at the other end of the connecting block 401 are both communicated with a second electric valve 419, the gas outlet ends of the two second electric valves 419 are respectively communicated with a three-way pipe 420 and an exhaust pipe 422, the remaining two ports of the three-way pipe 420 are respectively communicated with a third electric valve 421 and an auxiliary ring 423, the gas inlet end of the other first electric valve 414 is communicated with a processing box 424, the top of the processing box 424 is additionally provided with a box cover 426, the gas inlet end of the box cover 426 is communicated with a second connecting pipe 427, the inside of the top opening at one end of the connecting block 401 is provided with a plurality of first adsorption blocks 402 and a plurality of perforated partitions 403, the plurality of first adsorption blocks 402 and the plurality of perforated partitions 403 are stacked with each other, the gas inlet end of the one-way valve 405 is communicated with a circular pipe 406, the gas inlet end of the circular pipe 406 is communicated with an arc-shaped pipe 407, the gas outlet end of the arc-shaped pipe 407 is fixedly penetrated through the outer wall of the shell 102, the connecting block 401 is fixedly arranged in the inside of the shell 102, a fixing block 409 is fixedly arranged between the two processing tanks 408, the fixing block 409 is additionally arranged on the upper side of the connecting block 401, the gas inlet end of the conveying pipe 415 is communicated with the gas outlet end of the top opening at one end of the connecting block 401, the gas inlet end of one of the first connecting pipes 417 is communicated with the gas outlet end of one of the processing tanks 408, the surface of the sealing ring 418 abuts against the surface of the opening at the other end of the connecting block 401, the gas outlet end of the third electric valve 421 is communicated with the gas inlet end of the other first connecting pipe 417, the gas outlet end of the exhaust pipe 422 is fixedly penetrated through the inner wall of the shell 102, the auxiliary ring 423 is additionally arranged on the inner wall of the shell 102, a second adsorption block 425 is arranged in the inside of the processing box 424, the gas inlet end of the second connecting pipe 427 is communicated with the gas outlet end of the other processing tank 408, the processing box 424 is additionally arranged on the outer wall of the connecting shell 412, an outer shell 2 is fixedly arranged at the heat dissipation hole of the outer wall of the shell 102, a baffle 3 and a sealing gasket 8 are arranged in the inside of the outer shell 2, the surface of the sealing gasket 8 is in contact with the outer wall of the shell 102,The surface of the connecting block 401 is fixed with a plurality of support plates 6, and the bottom of each support plate 6 is in contact with the bottom of the inner wall of the base 101. The upper side of the connecting block 401 is additionally provided with a fixing frame 5, which is used for stabilizing the conveying pipe 415 during work.

[0025] When the biological test operation of the sample is completed, the baffle 3 and the sealing gasket 8 attached to the baffle 3 are first placed back into the housing 2, then the inlet end of the arc-shaped tube 407 is connected to the outlet end of the tube for conveying the sterilizing gas, then the valve of one of the second electric valves 419, the fan 411 and the valve of one of the first electric valves 414 are started, at this time the started fan 411 will suck the sterilizing gas conveyed by the tube through the arc-shaped tube 407, then the sterilizing gas in the arc-shaped tube 407 will be conveyed into the inside of the circular tube 406, then into the inside of the one-way valve 405, then into the inside composed of the top opening at one end of the connecting block 401 and the top cover 404, so that the first adsorption block 402 is completely dried, then the sterilizing gas after being dried will be conveyed into the inside of the connecting shell 412 through the conveying tube 415, the opened first electric valve 414 and the communicating tube 413, then through the started fan 411, the flow guide shell 410, the air inlet at the other end of the connecting block 401 and the sealing ring 418, into the inside of the opened second electric valve 419, and finally through the three-way tube 420 and the auxiliary ring 423, uniformly distributed in the space composed of the shell 102 and the base 101, so as to completely cover and sterilize the key areas inside the instrument, when the key areas inside the instrument are completely covered and sterilized, the valve of one of the first electric valves 414 and one of the second electric valves 419 are closed, the valve of the other first electric valve 414, the valve of the other second electric valve 419 and the third electric valve 421 are opened, at this time the continued started fan 411 will suck the sterilizing gas remaining in the space composed of the shell 102 and the base 101 through the auxiliary ring 423, then the sucked sterilizing gas will be conveyed into the inside of the opened third electric valve 421 through the three-way tube 420, then into the inside of the other first connecting tube 417, then into the inside of one of the treatment tanks 408 through the corresponding check valve 416, at this time the sterilizing gas entering the inside of the treatment tank 408 will be preliminarily treated, then the generated gas will be conveyed into the inside of the other first connecting tube 417, then into the inside of the corresponding check valve 416, then into the inside of the other treatment tank 408, when the generated gas enters the inside of the treatment tank 408 again, the sterilizing gas remaining in the gas will be completely removed by reaction at this time, then the generated gas will be conveyed into the inside of the second connecting tube 427, then into the space composed of the box cover 426 and the treatment box 424, when the gas passes through the space, the second adsorption block 425 will adsorb the water in the gas at this time, then the dried gas will be conveyed into the inside of the other opened second electric valve 419 through the other opened first electric valve 414, the communicating tube 413, the connecting shell 412, the flow guide shell 410, the sealing ring 418, the started fan 411 and the air inlet at the other end of the connecting block 401,The gas is then transported to the exhaust pipe 422 and discharged into the environment. Simultaneously, the baffle 3 and the bonded sealing gasket 8 are removed from the outer casing 2 to ensure that ambient gas can normally enter the space formed by the casing 102 and the base 101. This method allows for comprehensive disinfection of critical areas inside the instrument and also treats residual disinfectant gas, thereby improving the efficiency of the fully automated sample-dispensing quantitative PCR instrument for biological testing.

[0026] The overall effect and working principle of the mechanism are as follows: In the initial stage, the power cord is connected to the power module 112 through the pre-reserved round hole on the outer wall of the shell 102 near the bottom. Then, the power cord is connected to the power supply equipment. Press the switch 122 button to start the entire fully automatic sample loading fluorescence quantitative PCR instrument and let the instrument enter the initial interface. Then, open the cover 103 and place the sample loading tip, tube stopper, reagent tube and sample tube on the sample rack 107. At the same time, place the PCR reaction tube on the temperature control oscillator 108. Then, inject the PCR premix into the reagent tube. Then, inject the sample solution to be tested into the sample tube. Then, store the positive and negative control sample data and some basic operating parameters, such as the vibration frequency of the temperature control oscillator 108, the wind speed of the fan 411 and the opening and closing time of the sterilization lamp 119, etc. into the storage module integrated on the control board 114. Finally, close the cover 103 and remove the baffle 3 and the sealing gasket 8 attached to the baffle 3 from the inside of the shell 2. In the detection stage, the operator directly presses the start button on the display module 120, at which time the display module 120 will cooperate with the control panel 114 to start the mechanical arm 104, the needle cleaner 109 and all the disinfection lamps 119 (ultraviolet light). Subsequently, the front end of the mechanical arm 104 will drive the suction head mounting and dismounting device 105 to move. When the suction head end of the suction head mounting and dismounting device 105 moves to the sample rack 107 and is docked together, the suction head end of the suction head mounting and dismounting device 105 will be fixed by the control box 106 and one of the hoses. After that, the suction head end will be moved to the needle cleaning groove of the needle cleaner 109 for cleaning, and after cleaning, it will be moved to the reagent tube for sucking the quantitative PCR premix solution, and then moved to the PCR reaction tube of the temperature-controlled oscillator 108. When the suction head end completes the position movement, the PCR premix solution in the suction head end will be injected into the PCR reaction tube. Subsequently, the suction head end will be moved to the square hole position on the warehouse cover 116, and then the suction head end will be released from the suction head mounting and dismounting device 105, so that the suction head end falls into the collection warehouse 115 for collection (the disinfection lamp 119 will disinfect the suction head end). After that, the above operation steps will be repeated, and the front end of the suction head mounting and dismounting device 105 will be fixed with a new suction head end. Then the new suction head end will be moved to the needle cleaning groove of the needle cleaner 109 for cleaning, and after cleaning, it will be moved to the sample tube for sucking the quantitative detection sample solution. After that, the detection sample solution will be injected into the PCR reaction tube containing the PCR premix solution, and the two solutions will be mixed together. When the detection sample solution completes the solution injection, the suction head end will be released and sent into the collection warehouse 115 for collection (the disinfection lamp 119 will disinfect the suction head end). Subsequently, the tube plug will be fixed by the suction head mounting and dismounting device 105, and then moved to the tube opening of the PCR reaction tube to block it. After the blocking operation is completed, the tube plug will be released, and then the mechanical arm 104 will be closed. The temperature-controlled oscillator 108 will be started, which will mix the two solutions in the PCR reaction tube. When the two solutions complete the mixing operation, the temperature-controlled oscillator 108 will be closed. Subsequently, the PCR reaction tube containing the mixed solution will be moved to the placement plate on the thermal cycler 110 by cooperation of the mechanical arm 104, the suction head mounting and dismounting device 105, the fixed relationship between the tube plug and the PCR reaction tube, the control box 106 and the corresponding two hoses. Then the tube plug will be released, and the suction head mounting and dismounting device 105 will be reset to the original position. After that, the thermal cycler 110 will be started to control the reaction temperature of the mixed solution in the PCR reaction tube. Then the fluorescence detector 111 will be started, which will emit excitation light to the PCR reaction tube and collect the reflected fluorescent signal.After the fluorescence detector 111 will be collected fluorescence signal first converted into digital signal, and then transmitted to the control board 114, the control board 114 after receiving the data, will first be processed, and then draw the fluorescence amplification curve, while calculating the Ct value, finally the control board 114 will be compared with the Ct value and the prior storage of positive and negative control sample data, to get the test results of the test sample, when the test results processing, the control board 114 will transmit the test results to the display module 120, and displayed on the screen of the display module 120, while the test results in the control board 114 will be integrated into the storage module storage, for later viewing; Disinfection stage, when the detection sample completes the biological test operation, at this time the baffle 3 and the sealing gasket 8 bonded on the baffle 3 are placed back to the inside of the shell 2, then the air inlet end of the arc-shaped tube 407 is connected with the gas outlet end of the pipe conveying disinfectant gas (dry chlorine dioxide gas), then the valve of one of the second electric valves 419, the fan 411 and the valve of one of the first electric valves 414 are started, at this time the started fan 411 will suck the disinfectant gas conveyed by the pipe through the arc-shaped tube 407, then the disinfectant gas entering the inside of the arc-shaped tube 407 will be conveyed to the inside of the circular tube 406 first, then to the inside of the one-way valve 405, then to the inside composed of the top opening at one end of the connecting block 401 and the top cover 404, so that the first adsorption block 402 (3A molecular sieve particles) can be completely dried, then the dried disinfectant gas will be conveyed to the inside of the connecting shell 412 through the conveying pipe 415 and the opened first electric valve 414 and the communication pipe 413, then through the started fan 411, the flow guide shell 410, the air inlet at the other end of the connecting block 401 and the sealing ring 418, to the inside of the opened second electric valve 419, finally through the three-way pipe 420 and the auxiliary ring 423, evenly distributed in the space composed of the shell 102 and the base 101, to completely cover the key areas inside the instrument for disinfection; Residual gas treatment stage, when the key area inside the instrument is fully covered with disinfectant, at this time one of the first electric valve 414 and one of the second electric valve 419 is closed, the other first electric valve 414 and the other second electric valve 419 are opened, and the third electric valve 421 is opened. At this time, the fan 411 is started, and the space composed of the shell 102 and the base 101 is connected through the auxiliary ring 423, and the residual disinfectant gas in the space is removed. Subsequently, the removed disinfectant gas is transported into the third electric valve 421 through the three-way pipe 420, and then into the other first connecting pipe 417, and then into the other processing tank 408 through the corresponding check valve 416. At this time, the disinfectant gas entering the processing tank 408 is preliminarily treated (urea solution is injected into the processing tank 408 in advance), and then the reaction gas is transported into the first connecting pipe 417, the corresponding check valve 416 and the other processing tank 408. When the generated gas enters the processing tank 408 again, the residual disinfectant gas in the gas is completely removed by reaction, and then the generated gas is transported into the second connecting pipe 427, and then into the space composed of the box cover 426 and the processing box 424. When the gas passes through the space, the second adsorption block 425 (composed of activated carbon particles) adsorbs the water in the gas. Subsequently, the dried gas is transported into the other first electric valve 414, the connecting pipe 413, the connecting shell 412, the flow guide shell 410, the sealing ring 418, the fan 411 and the other end of the connecting block 401 through the other first electric valve 414, the connecting pipe 413, the connecting shell 412, the flow guide shell 410, the sealing ring 418, the fan 411 and the other end of the connecting block 401. The gas is transported into the second electric valve 419, and then into the exhaust pipe 422, and then into the environment. At the same time, the baffle 3 and the sealing gasket 8 bonded thereto are taken out of the shell 2 to ensure that the gas in the environment can normally enter the space composed of the shell 102 and the base 101.

[0027] The suction head mounting and dismounting device 105 is connected to the control box 106 through two hoses, one end of each elastic rope 7 is connected to each hose, and the two hoses are placed on the auxiliary rod 123. The elastic rope 7 is used to control the placement of the hose.

[0028] Among them, the mechanical arm 104, the suction head loading and unloading device 105, the control box 106, the temperature control oscillator 108, the needle washing device 109, the thermal cycler 110, the fluorescence detector 111, the power module 112, the control panel 114, the electromagnet 118, the disinfection lamp 119, the display module 120, the alarm 121, the switch 122, the fan 411, the first electric valve 414, the second electric valve 419 and the third electric valve 421 all belong to the disclosed technology, and the wiring diagram between these components also belongs to the disclosed technology in the art, and the type can be selected according to the actual situation, so the control mode and the wiring condition between these components are not described in detail here.

[0029] Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacements for part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. Fully automatic sample loading fluorescence quantitative PCR instrument based on biological test, comprising an analysis mechanism (1), characterized in that: The analysis mechanism (1) includes a base (101) and a shell (102), the inside of the shell (102) is provided with a processing mechanism (4), the processing mechanism (4) is used for disinfection of all components inside the shell (102) and processing of residual disinfection gas; The processing mechanism (4) includes a connecting block (401), a top opening of one end of the connecting block (401) is provided with a top cover (404), a gas inlet end of the top cover (404) is communicated with a one-way valve (405), an upper side of the connecting block (401) is provided with two processing tanks (408), the upper side of the connecting block (401) is provided with a flow guide shell (410) and a connecting shell (412), a fan (411) is arranged between the inside of the flow guide shell (410) and the inside of the connecting shell (412), a gas inlet end of the connecting shell (412) is communicated with a communication pipe (413), the remaining two gas inlet ends of the communication pipe (413) are communicated with first electric valves (414), a gas inlet end of one of the first electric valves (414) is communicated with a conveying pipe (415), gas inlet ends of the two processing tanks (408) are communicated with check valves (416), gas inlet ends of the two check valves (416) are communicated with first connecting pipes (417), a sealing ring (418) is bonded to a gas outlet end surface of the flow guide shell (410), two gas outlet openings of the other end of the connecting block (401) are communicated with second electric valves (419), gas outlet ends of the two second electric valves (419) are respectively communicated with a three-way pipe (420) and an exhaust pipe (422), the remaining two ports of the three-way pipe (420) are respectively communicated with a third electric valve (421) and an auxiliary ring (423), a gas inlet end of the other first electric valve (414) is communicated with a processing box (424), a top of the processing box (424) is provided with a box cover (426), a gas inlet end of the box cover (426) is communicated with a second connecting pipe (427).

2. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 1, characterized in that: A plurality of first adsorption blocks (402) and a plurality of perforated partitions (403) are arranged in the inside of the top opening of one end of the connecting block (401), the plurality of first adsorption blocks (402) and the plurality of perforated partitions (403) are stacked with each other, a gas inlet end of the one-way valve (405) is communicated with a circular pipe (406), and a gas inlet end of the circular pipe (406) is communicated with an arc-shaped pipe (407).

3. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 2, characterized in that: The gas outlet end of the arc-shaped pipe (407) is fixedly penetrated through the outer wall of the shell (102), the connecting block (401) is fixed in the inside of the shell (102), a fixed block (409) is fixed between the two processing tanks (408), the fixed block (409) is arranged on the upper side of the connecting block (401), and the gas inlet end of the conveying pipe (415) is communicated with the gas outlet end of the top opening of one end of the connecting block (401).

4. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 1, characterized in that: One of the first connecting pipe (417) intake end and one of the processing tank (408) gas outlet connected, the surface of the sealing ring (418) and the other end of the connecting block (401) corresponding intake port surface, the third electric valve (421) gas outlet and another first connecting pipe (417) intake end connected.

5. The fully automated sample loading bioassay-based fluorogenic quantitative PCR instrument according to claim 1, characterized in that: The exhaust pipe (422) gas outlet fixed through the inner wall of the shell (102), the auxiliary ring (423) is installed in the inner wall of the shell (102), the processing box (424) is placed in the second adsorption block (425), the second connecting pipe (427) intake end and another processing tank (408) gas outlet connected, the processing box (424) is installed in the outer wall of the connecting shell (412).

6. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 1, characterized in that: The base (101) is installed at the bottom of the shell (102), the top opening of the shell (102) is rotatably connected with the cover plate (103), the inner wall of the base (101) is installed with the mechanical arm (104) at the bottom, and the front end of the mechanical arm (104) is installed with the suction head loading and unloading device (105).

7. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 1, characterized in that: The inner wall of the base (101) is installed with the control box (106), the sample rack (107), the temperature control oscillator (108), the needle washing device (109), the thermal cycler (110) and the power module (112) at the bottom, the thermal cycler (110) is installed with the fluorescence detector (111), the inner wall of the base (101) is fixed with a plurality of hexagonal columns (113) at the bottom, and the control panel (114) is installed between the top of the plurality of hexagonal columns (113).

8. The fully automated sample loading bioassay-based fluorogenic quantitative PCR instrument according to claim 1, characterized in that: The side square hole of the shell (102) is movably sleeved with the collection bin (115), the top of the collection bin (115) is installed with the bin cover (116), the inner wall of the base (101) is fixed with the mounting bracket (117) at the bottom, the mounting bracket (117) is installed with the electromagnet (118), the magnetic suction end of the electromagnet (118) is magnetically connected with the outer wall of the collection bin (115), and the inside of the collection bin (115) is provided with a plurality of disinfection lamps (119), and the plurality of disinfection lamps (119) are installed at the bottom of the bin cover (116).

9. The fully automated sample loading bioassay-based fluorogenic quantitative PCR instrument according to claim 1, characterized in that: The inside of the shell (102) is installed with the display module (120), the display end of the display module (120) is movably penetrated through the inner wall of the shell (102), the top of the side flat plate of the shell (102) is installed with the alarm (121), the inner wall of the shell (102) is installed with the switch (122), the control end of the switch (122) is movably penetrated through the inner wall of the shell (102), and the inside of the shell (102) is installed with the auxiliary rod (123).

10. The fully automated sample loading bioassay-based fluorescent quantitative PCR instrument according to claim 7, characterized in that: The outer wall of the shell (102) is fixed with an outer shell (2), the inside of the outer shell (2) is placed with a baffle (3) and a sealing gasket (8), the surface of the sealing gasket (8) is in contact with the outer wall of the shell (102), the surface of the connecting block (401) is fixed with a plurality of supporting plates (6), and the bottom of each supporting plate (6) is in contact with the bottom of the inner wall of the base (101), the upper side of the connecting block (401) is additionally provided with a fixing frame (5), the fixing frame (5) is used for stabilizing the conveying pipe (415) during work, and the top of the control box (106) is fixed with an elastic rope (7).

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