Supernatant suction device and method for chromosome distortion detection

Abstract

The invention discloses a supernate suction device and method for chromosome aberration detection, and belongs to the technical field of biological detection liquid purification. The supernate suction device comprises a mounting box, a placement plate for placing a centrifugal tube is arranged at the bottom of the mounting box, and a U-shaped sliding plate is slidably connected into the mounting box; a plurality of pipettes coaxially arranged with the centrifugal tubes one by one are fixedly connected to the sliding plate, pistons capable of being fixed relative to the mounting box are arranged in the pipettes, the pipettes are in sliding connection with the pistons, pipette heads are arranged at the ends, close to the centrifugal tubes, of the pipettes, the other ends of the pipette heads are closed, and a plurality of pipette holes are formed in the side walls of the pipette heads; a baffle is fixedly connected to the liquid suction head, an annular groove is formed between the baffle and the liquid suction head, and a pressure relief channel is formed in the baffle. According to the invention, the baffle plate is arranged, and the mode that the piston does not move and the liquid suction pipe moves downwards is utilized to suck the supernatant, so that the sediment is prevented from being disturbed by suction force due to the fact that the supernatant is sucked at the junction of the supernatant and the sediment.

Description

Technical Field

[0001] This invention belongs to the field of biological detection solution purification technology, specifically relating to a device and method for extracting supernatant for chromosomal aberration detection. Background Technology

[0002] In biomedical testing, a key step is the purification of the biological testing solution. A typical procedure involves centrifugation followed by collection of the precipitate. Taking the purification of chromosomal aberration detection solution as an example, the traditional steps are as follows: First, the test solution (cultured blood) is injected into a centrifuge tube. Then, an appropriate amount of sodium chloride solution (a reaction solution) is added and mixed / suspended. A first centrifugation is then performed, and the supernatant is collected. Next, an appropriate amount of mixed acid (a mixture of methanol and glacial acetic acid, also a reaction solution) is added to the centrifuge tube and mixed / suspended. A second centrifugation is then performed, and the supernatant is collected.

[0003] Existing technologies, such as the Chinese patent with publication number CN116393190A, disclose an intelligent suspension pipetting device and a method for purifying chromosomal aberration detection solutions. This involves inserting a syringe assembly into a centrifuge tube to aspirate the supernatant. However, in this technology, when aspirating the supernatant, the lower end of the syringe (i.e., the needle tip) needs to be moved to the interface between the supernatant and the precipitate. Then, the piston rod is controlled to move to aspirate the supernatant. Because the needle tip is very close to the precipitate, the negative pressure generated by the suction can easily stir up the precipitate, causing it to remix with the supernatant, rendering the precipitate ineffective, or even causing the precipitate to clog the syringe.

[0004] Therefore, it is necessary to propose a device and method for supernatant aspiration in the detection of chromosomal aberrations to solve the above problems. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a supernatant aspiration device and method for chromosomal aberration detection, which solves the problem in the prior art that moving the lower end of the needle to the interface between the supernatant and the precipitate to aspirate the supernatant easily causes the precipitate to be disturbed by suction, resulting in precipitate failure or even blockage of the needle.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a supernatant aspiration device for chromosomal aberration detection, comprising a mounting box, a placement plate for arraying centrifuge tubes slidably disposed at the bottom of the mounting box, a U-shaped sliding plate slidably connected inside the mounting box, and a plurality of aspiration tubes coaxially arranged with each centrifuge tube fixedly connected to the sliding plate. Sliding the sliding plate allows the aspiration tubes to extend into the centrifuge tubes. A gap is provided between the outer wall of the aspiration tube and the inner wall of the centrifuge tube. A piston that can be fixed relative to the mounting box is disposed inside the aspiration tube. The aspiration tube is slidably connected to the piston. An aspiration head is disposed near the end of the aspiration tube of the centrifuge tube. The end of the aspiration head away from the aspiration tube is closed. A plurality of aspiration holes are circumferentially disposed on the side wall of the aspiration head with the axis of the aspiration head as the center. A baffle that matches the inner diameter of the centrifuge tube is fixedly connected to the aspiration head. An annular groove is provided between the baffle and the aspiration head. A pressure relief channel is provided on the baffle.

[0007] Furthermore, a support plate is fixedly connected inside the mounting box, and a sealing sleeve that is slidably connected to the inner wall of the suction tube is fixedly connected to the support plate. An injection tube that is coaxially arranged with the suction tube is slidably connected inside the sealing sleeve. One end of the injection tube is fixedly connected to the piston. When the injection tube slides down to the lower limit position, it can fix the piston and the mounting box relative to each other. A fixing plate that can seal the suction tube is fixedly connected between the sealing sleeve and the piston inside the suction tube. The injection tube passes through the fixing plate and is slidably connected to the fixing plate. A pressure relief hole that connects the squeezing chamber and the outside of the suction tube is provided on the fixing plate.

[0008] Furthermore, the piston is fixedly connected to a telescopic tube communicating with the injection tube at the end away from the piston. The telescopic tube at the end away from the piston is fixedly connected to the suction head at the end away from the suction tube. An annular gap is provided between the telescopic tube and the suction head. A one-way valve is provided inside the telescopic tube to allow the liquid in the injection tube to flow out of the telescopic tube in one direction. The one-way valve can close the suction head at the end away from the suction tube. A storage chamber for storing the reaction liquid is formed between the fixed plate and the sealing sleeve. A squeezing chamber is formed between the fixed plate and the piston. The injection tube is provided with a strip channel communicating with the storage chamber. A flexible tube is fixedly provided on the sealing sleeve. The outlet chamber is connected to the reaction liquid storage tank through the flexible tube. A solenoid valve is provided on the flexible tube.

[0009] Furthermore, a connecting sleeve is fixedly installed on the piston, and the injection tube is inserted into the connecting sleeve and fixedly connected to the piston. A through hole for the injection tube to pass through is provided in the fixing plate, and an elastic sleeve surrounding the injection tube is fixedly installed on the inner wall of the through hole. The elastic sleeve can be slidably fitted onto the connecting sleeve by sliding the fixing plate along the injection tube.

[0010] Furthermore, a stop block that cooperates with the strip channel is fixedly provided on the sealing sleeve. When the injection tube moves down to the top wall of the strip channel and abuts against the stop block, the injection tube is in the lower limit position.

[0011] Furthermore, a first cylinder is fixedly installed on the top of the mounting box. The output end of the first cylinder is fixedly connected to a push rod that can extend into the closed sleeve. The first cylinder drives the push rod to move downward, which can squeeze the injection tube to move downward. When the first cylinder pushes the push rod to the maximum stroke, the injection tube is at the lower limit position.

[0012] Furthermore, an upper connecting part and a lower connecting part are fixedly provided on the fixing plate, and the suction tube includes an upper part of the suction tube that is threadedly connected to the upper connecting part and a lower part of the suction tube that is threadedly connected to the lower connecting part; a sealing gasket is provided between the upper part of the suction tube and the lower part of the suction tube and the fixing plate.

[0013] Furthermore, a retaining ring is fixedly sleeved on the injection tube below the strip channel, and the retaining ring is positioned above the fixing plate.

[0014] Furthermore, a second cylinder is fixedly installed on the top of the mounting box, and the output end of the second cylinder is fixedly connected to the slide plate.

[0015] A method for aspirating supernatant using a chromosomal aberration detection device includes the following steps: S1: Initial state adjustment, the first cylinder outputs thrust to push the push rod to move the injection tube and piston down to the lower limit position, then the first cylinder outputs pull force to disengage the push rod from the injection tube so that the injection tube can slide upward; and the position of the slide plate is adjusted by the second cylinder so that the suction head is above the centrifuge tube; S2: After injecting the test solution into the centrifuge tube and adding the reaction solution for the first centrifugation, place it on the placement plate and add the preset amount of reaction solution to the reaction solution storage tank. S3: Start the second cylinder to drive the slide plate to move down at a constant speed, so that the suction tube moves closer to the centrifuge tube until the baffle moves to the junction of the supernatant and the precipitate. At this time, the elastic sleeve moves down synchronously and is fitted onto the connecting sleeve. When the suction tube moves down, the supernatant is sucked into the suction space by negative pressure, and the solenoid valve is opened so that all the reaction liquid in the reaction liquid storage tank flows into the storage chamber. At this time, the reaction liquid storage tank is set up in an open position. S4: Start the second cylinder to drive the slide plate to move upward at a constant speed and close the solenoid valve so that the suction pipe, the fixed plate and the piston move upward synchronously. At this time, the volume in the suction space remains unchanged, and the injection pipe moves upward along the closed sleeve so that the volume of the storage chamber becomes smaller so that the liquid flows into the injection pipe from the strip channel. Under the action of pressure, the reaction liquid opens the one-way valve so that the reaction liquid forms a jet impact precipitate. S5: Remove the placement plate for secondary centrifugation, and place the collection tank under the suction pipe. Then start the first cylinder to push the push rod to push the injection pipe and piston to squeeze the supernatant in the suction space into the collection tank. S6: Place the centrifuge tubes after secondary centrifugation into the mounting box, and open the solenoid valve to connect the storage chamber with the outside. Then, start the second cylinder to move the suction tube down to draw up the supernatant, and then move the suction tube up to remove the placement plate. Then, start the first cylinder to push the piston to squeeze the supernatant in the suction space into the collection tank. During the downward and upward movement of the suction tube, the storage chamber is connected to the outside so that the one-way valve always closes the telescopic tube.

[0016] The beneficial effects of this invention are as follows: By setting a baffle and using a method where the piston remains stationary while the suction tube moves downward, a negative pressure is created in the suction space to draw in the supernatant. The downward movement of the baffle ensures that it remains in contact with the surface of the supernatant, preventing suction from disturbing the precipitate at the interface between the supernatant and the precipitate. Furthermore, the radially arranged suction holes on the suction head allow for side suction of the supernatant entering the annular groove, avoiding disturbance to the precipitate caused by direct suction when close to it. By setting a fixed plate and a liquid injection tube to work together, the fixed plate can slide and fit on the connecting sleeve when it moves down to near the piston, thereby increasing the friction between the liquid suction tube and the piston. This allows the piston to move up synchronously when the liquid suction tube moves up, ensuring the pressure of the suction space is stable and preventing the supernatant from overflowing when the liquid suction tube moves up. The fixed plate and the closed sleeve form a liquid storage chamber between them, which facilitates the mixing of the injected reaction liquid with the precipitate as the suction tube moves upward, thus improving the mixing efficiency of the secondary mixing. During the injection of the reaction liquid, the baffle moves upward synchronously with the liquid, which can avoid splashing of the mixture caused by the reaction liquid jet and improve the uniformity of the mixing between the reaction liquid and the precipitate. Furthermore, during the first downward and upward movement of the suction tube, the supernatant can be drawn up and the second reaction liquid can be mixed, thus improving efficiency.

[0017] Other advantages, objectives, and features of the invention will be set forth in the following description and will be apparent to those skilled in the art in some respects, or may be learned by practice of the invention. The objectives and other advantages of the invention can be realized and obtained through the following description. Attached Figure Description

[0018] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the following figures are provided for illustration: Figure 1 This is a partial cross-sectional view of the supernatant aspiration device according to an embodiment of the present invention; Figure 2 Embodiments of the present invention Figure 1 A magnified view of part A in the middle; Figure 3 Embodiments of the present invention Figure 1 A magnified view of part B in the middle; Figure 4 Embodiments of the present invention Figure 1 A magnified view of part C in the middle; Figure 5 This is a schematic diagram of the overall structure of the supernatant suction device according to an embodiment of the present invention.

[0019] The following components are labeled in the attached diagram: mounting box 1, support plate 101, first cylinder 102, push rod 103, second cylinder 104, centrifuge tube 105, placement plate 2, sliding plate 3, suction tube 4, piston 401, suction head 402, suction hole 403, baffle 404, annular groove 405, suction space 406, storage chamber 407, squeezing chamber 408, connecting sleeve 409, upper part of suction tube 410, lower part of suction tube 411, pressure relief channel 412, sealing sleeve 5, hose 501, stop block 502, injection tube 6, strip channel 601, retaining ring 602, telescopic tube 7, fixing plate 8, pressure relief hole 801, elastic sleeve 802, upper connecting part 803, lower connecting part 804, sealing gasket 805. Detailed Implementation

[0020] like Figure 1 and Figure 4 As shown, the present invention provides a supernatant aspiration device for chromosomal aberration detection, comprising: a mounting box 1, wherein a placement plate 2 for placing centrifuge tubes 105 is slidably disposed at the bottom of the mounting box 1, the placement plate 2 being able to slide out along the mounting box 1; a U-shaped sliding plate 3 is slidably connected inside the mounting box 1, wherein the two side walls of the sliding plate 3 are slidably connected to the inner wall of the mounting box 1 to improve the sliding stability of the sliding plate 3; a suction tube 4 coaxially disposed with the centrifuge tubes 105 is fixedly connected to the sliding plate 3, and sliding the sliding plate 3 allows the suction tube 4 to extend into the centrifuge tubes 105; a space is provided between the outer wall of the suction tube 4 and the inner wall of the centrifuge tubes 105. In the gap, a piston 401 is provided inside the suction tube 4, which can be fixedly connected to the mounting box 1. The suction tube 4 and the piston 401 are slidably connected. A suction head 402 is provided at the end of the suction tube 4 near the centrifuge tube 105. The end of the suction head 402 away from the suction tube 4 is closed. Multiple suction holes 403 are provided circumferentially around the axis of the suction head 402 on the side wall. A baffle 404 that matches the inner diameter of the centrifuge tube 105 is fixedly connected to the suction head 403. An annular groove 405 is provided between the baffle 404 and the suction head 403. A pressure relief channel 412 is provided on the baffle 404.

[0021] In this design, when aspirating the supernatant, the sliding plate 3 moves the suction tube 4 closer to the centrifuge tube 105 until the baffle 404 moves into the centrifuge tube 105. During the movement of the sliding plate 3, since the piston 401 is in a fixed state, the suction space 406 formed between the suction head 402 and the piston 401 increases. When the suction tube 4 moves into the centrifuge tube 105 and the baffle 404 contacts the surface of the supernatant, continuing to slide the suction tube 4 allows the supernatant to be drawn into the suction space 406 under negative pressure, thereby achieving the aspiration of the supernatant. During the movement of the baffle 404, since the diameter of the baffle 404 is the same as the inner diameter of the centrifuge tube 105, the baffle 404 can seal the centrifuge tube 105. When the baffle 404 contacts the surface of the supernatant, some of the liquid can be squeezed into the annular groove 405 under pressure. This allows the negative pressure in the suction space 406 to draw the supernatant in laterally through the suction hole 403, thus avoiding disturbance to the precipitate caused by direct suction when the baffle 404 moves to the surface of the precipitate. Furthermore, before the baffle 404 contacts the supernatant in the centrifuge tube 105, pressure is released through the pressure relief channel 412 to prevent the pressure inside the centrifuge tube 105 from increasing due to the baffle 404 closing the centrifuge tube 105. When the baffle 404 contacts the supernatant, since the space of the annular groove 405 is larger than the pressure relief channel 412, the supernatant can rise into the annular groove 405 first and be drawn into the suction space 406 by negative pressure.

[0022] like Figure 2 and Figure 3As shown, in one embodiment of the present invention, a support plate 101 is fixedly connected inside the mounting box 1. A sealing sleeve 5, which is slidably connected to the inner wall of the suction tube 4, is fixedly connected to the support plate 101. An injection tube 6, coaxially arranged with the suction tube 4, is slidably connected inside the sealing sleeve 5. One end of the injection tube 6 is fixedly connected to the piston 401. When the injection tube 6 slides downward to its limit position, it can fix the piston 401 relative to the mounting box 1. A telescopic tube 7, communicating with the injection tube 6, is fixedly connected to the end of the piston 401 away from the injection tube 6. The end of the telescopic tube 7 away from the piston 401 is fixedly connected to the end of the suction head 402 away from the suction tube 4. An annular gap is provided between the telescopic tube 7 and the suction head 402. A one-way valve (not shown in the figure) is provided inside the telescopic tube 7, which allows the liquid in the injection tube 6 to flow out of the telescopic tube 7 in one direction. The one-way valve allows the liquid to be drawn out. The head 403 is closed away from the end of the suction tube 4. A fixing plate 8 that can close the suction tube 4 is fixedly connected between the sealing sleeve 5 and the piston 401. The injection tube 6 passes through the fixing plate 8 and is slidably connected to the fixing plate 8. A storage chamber 407 for storing the reaction liquid is formed between the fixing plate 8 and the sealing sleeve 5. A squeezing chamber 408 is formed between the fixing plate 8 and the piston 401. A pressure relief hole 801 is provided on the fixing plate 8 to connect the squeezing chamber 408 with the outside of the suction tube 4. A strip channel 601 is provided on the injection tube 6 to communicate with the storage chamber 407. A hose 501 is fixedly provided on the sealing sleeve 5. The storage chamber 407 is connected to a reaction liquid storage tank (not shown in the figure) through the hose 501. A solenoid valve (not shown in the figure) is provided on the hose 501 to allow the reaction liquid in the reaction liquid storage tank to flow unidirectionally into the storage chamber 407.

[0023] In this design, when the supernatant is drawn, the injection tube 6 is at its lower limit position. The sliding plate 3 moves the suction tube 4 closer to the centrifuge tube 105, thereby causing the fixed plate 8 to move synchronously with the suction tube 4. The pressure relief hole 801 stabilizes the pressure in the squeezing chamber 408, preventing the fixed plate 8 from being difficult to slide. During the downward movement of the fixed plate 8, the friction between it and the injection tube 6 applies a downward force to the injection tube 6, keeping it at its lower limit position and fixing the piston 401. As the suction tube 4 slides downward, the suction space 406 increases and the telescopic tube 7 is stretched. During the contact and downward movement of the suction tube 4 with the supernatant, the supernatant is drawn into the suction space 406 until the upper... After the supernatant is aspirated, slide the slide plate 3 upward. Due to the large friction between the suction pipe 4 and the piston 401, as the suction pipe 4 moves upward, the injection pipe 6 can slide upward along the closed sleeve 5, thereby driving the piston 401 to move upward synchronously. This keeps the volume of the suction space 406 unchanged, preventing the supernatant from being squeezed out. As the suction pipe 4 moves downward, it increases the volume of the storage chamber 407, allowing the reaction liquid in the reaction liquid storage tank to flow into the storage chamber 407. As the suction pipe 4 moves upward, it squeezes the storage chamber 407, causing the reaction liquid to flow from the strip channel 601 into the injection pipe 6. Under pressure, the reaction liquid opens the one-way valve, allowing the reaction liquid to form a jet impact to precipitate and mix, and then undergo secondary centrifugation.

[0024] In one embodiment of the present invention, a connecting sleeve 409 is fixedly provided on the piston 401, and the injection tube 6 is inserted into the connecting sleeve 409 and fixedly connected to the piston 401. The diameter of the connecting sleeve 409 is slightly larger than the diameter of the injection tube 6. A through hole for the injection tube 6 to pass through is provided in the fixing plate 8, and an elastic sleeve 802 surrounding the injection tube 6 is fixedly provided on the inner wall of the through hole.

[0025] In this design, the elastic sleeve 802 increases the friction between the elastic sleeve 802 and the injection tube 6, thereby increasing the downward pulling force on the injection tube 6 during the downward movement of the fixed plate 8. This ensures that the injection tube 6 remains at its lower limit position during the downward movement of the fixed plate 8, and as the fixed plate 8 moves closer to the piston 401, the elastic sleeve 802 slides onto the connecting sleeve 409, thus increasing the squeezing force of the elastic sleeve 802 on the connecting sleeve 409. This further enhances the friction between the suction tube 4 and the piston 401 during the upward movement of the suction tube 4, ensuring that the upward movement of the suction tube 4 can drive the piston 401 to move upward synchronously.

[0026] In one embodiment of the present invention, a stop 502 that cooperates with the strip channel 601 is fixedly provided on the sealing sleeve 5. When the injection tube 6 moves down to the top wall of the strip channel 601 and abuts against the stop 502, the injection tube 6 is in the lower limit position.

[0027] In this design, the baffle 502 provides a stop for the downward movement limit of the injection tube 6 and prevents the reaction liquid from being carried into the sealing sleeve 5 during the upward movement of the injection tube 6.

[0028] In one embodiment of the present invention, a first cylinder 102 is fixedly installed on the top of the mounting box 1. The output end of the first cylinder 102 is fixedly connected to a push rod 103 that can extend into the closed sleeve 5. The first cylinder 102 drives the push rod 103 to move downward, which can squeeze the injection tube 6 to move downward. When the first cylinder 102 pushes the push rod 103 to move to the maximum stroke, the injection tube 6 is at the lower limit position.

[0029] In this scheme, after the liquid aspiration is completed, the push rod 103 is moved by the first cylinder 102 to push the injection tube 6 and the piston 401 to move down and squeeze out the supernatant in the liquid aspiration space 406.

[0030] like Figure 3 As shown, in one embodiment of the present invention, the fixing plate 8 is fixedly provided with an upper connecting part 803 and a lower connecting part 804, and the suction tube 4 includes an upper suction tube 410 threadedly connected to the upper connecting part 803 and a lower suction tube 411 threadedly connected to the lower connecting part 804.

[0031] In this solution, the design of the above structure facilitates the installation and disassembly of the suction tube 4. The upper part 401 or the lower part 411 of the suction tube can be disassembled separately for easy maintenance and replacement.

[0032] In one embodiment of the present invention, a sealing gasket 805 is provided between the upper part 410 and the lower part 411 of the suction tube and the fixing plate 8 to improve the sealing performance of the connection between the suction tube 4 and the fixing plate 8.

[0033] In one embodiment of the present invention, a retaining ring 602 located below the strip channel 601 is fixedly sleeved on the injection tube 6, and the retaining ring 602 is located above the fixing plate 8.

[0034] In this design, the baffle ring 602 is used to prevent the fixed plate 8 from moving above the strip channel 601 when it moves upward along the injection pipe 6, thus preventing the reaction liquid from flowing into the squeezing chamber 408.

[0035] like Figure 5 As shown, in one embodiment of the present invention, multiple centrifuge tubes 105 are arranged in an array on the placement plate 2 to improve the liquid aspiration efficiency. A second cylinder 104 is fixedly installed on the top of the mounting box 1. The output end of the second cylinder 104 is fixedly connected to the slide plate 3. The second cylinder 104 can drive the slide plate 3 to move, thereby driving multiple liquid aspiration tubes 4 to move synchronously.

[0036] A method for aspirating supernatant using a chromosomal aberration detection device includes the following steps: S1: Initial state adjustment, the first cylinder 102 outputs thrust to push the push rod 103 to push the injection tube 6 and piston 401 down to the lower limit position, then the first cylinder 102 outputs pull force to disengage the push rod 103 from the injection tube 6 so that the injection tube 6 can slide upward; and the position of the slide plate 3 is adjusted by the second cylinder 104 so that the suction head 402 is above the centrifuge tube 105; S2: Inject the test solution into centrifuge tube 105 and add reaction solution for the first centrifugation treatment. Then place it on the placement plate 2 and add the preset amount of reaction solution to the reaction solution storage tank. S3: Start the second cylinder 104 to drive the slide plate 3 to move down at a constant speed, so that the suction tube 4 moves towards the centrifuge tube 105 until the baffle 404 moves to the junction of the supernatant and the precipitate. At this time, the elastic sleeve 802 moves down synchronously and is fitted onto the connecting sleeve 409. When the suction tube 4 moves down, the supernatant is sucked into the suction space 406 by negative pressure, and the solenoid valve is opened so that all the reaction liquid in the reaction liquid storage tank flows into the storage chamber 407. At this time, the reaction liquid storage tank is set open. S4: Start the second cylinder 104 to drive the slide plate 3 to move upward at a constant speed and close the solenoid valve so that the suction pipe 4, the fixed plate 8 and the piston 401 move upward synchronously. At this time, the volume in the suction space 406 remains unchanged, and the injection pipe 6 moves upward along the closed sleeve 5 so that the volume of the storage chamber 407 becomes smaller so that the liquid flows into the injection pipe 6 from the strip channel 601. Under the action of pressure, the reaction liquid opens the one-way valve so that the reaction liquid forms a jet impact precipitate. S5: Take out the placement plate 2 for secondary centrifugation, and place the collection tank under the suction pipe 4. Then start the first cylinder 102 so that the push rod 103 pushes the injection pipe 6 and the piston 401 to squeeze the supernatant in the suction space 406 into the collection tank. S6: Place the centrifuge tube 105 after secondary centrifugation into the mounting box 1, and open the solenoid valve to connect the liquid storage chamber 407 with the outside. Then, start the second cylinder 104 to drive the suction tube 4 to move down and suck up the supernatant. Then, drive the suction tube 4 to move up and take out the placement plate 2. Then, start the first cylinder 102 to push the piston 401 to squeeze the supernatant in the suction space 406 into the collection tank. During the downward and upward movement of the suction tube 4, the liquid storage chamber 407 is connected to the outside so that the one-way valve always closes the telescopic tube 7.

[0037] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.

Claims

1. A device for aspirating supernatant for detecting chromosome aberrations, comprising a mounting box, characterized in that: The bottom of the mounting box is slidably equipped with a placement plate for arranging centrifuge tubes in an array. A U-shaped sliding plate is slidably connected inside the mounting box. Multiple suction tubes, coaxially arranged with each centrifuge tube, are fixedly connected to the sliding plate. Sliding the sliding plate allows the suction tubes to extend into the centrifuge tubes. A gap is provided between the outer wall of the suction tube and the inner wall of the centrifuge tube. A piston that can be fixed relative to the mounting box is provided inside the suction tube. The suction tube and the piston are slidably connected. A suction head is provided near the end of the suction tube near the centrifuge tube. The end of the suction head away from the suction tube is closed. Multiple suction holes are provided circumferentially around the axis of the suction head on the side wall. A baffle that matches the inner diameter of the centrifuge tube is fixedly connected to the suction head. An annular groove is provided between the baffle and the suction head. A pressure relief channel is provided on the baffle.

2. The supernatant aspiration device for chromosome aberration detection according to claim 1, characterized in that: A support plate is fixedly connected inside the mounting box. A sealing sleeve that is slidably connected to the inner wall of the suction tube is fixedly connected to the support plate. An injection tube that is coaxially arranged with the suction tube is slidably connected inside the sealing sleeve. One end of the injection tube is fixedly connected to the piston. When the injection tube slides down to the lower limit position, it can fix the piston and the mounting box relative to each other. A fixing plate that can seal the suction tube is fixedly connected between the sealing sleeve and the piston inside the suction tube. The injection tube passes through the fixing plate and is slidably connected to the fixing plate. A pressure relief hole that connects the squeezing chamber and the outside of the suction tube is provided on the fixing plate.

3. The supernatant aspiration device for chromosome aberration detection according to claim 2, characterized in that: The piston is fixedly connected to a telescopic tube communicating with the injection tube at the end away from the piston. The telescopic tube at the end away from the piston is fixedly connected to the suction head at the end away from the suction tube. An annular gap is provided between the telescopic tube and the suction head. A one-way valve is provided inside the telescopic tube to allow the liquid in the injection tube to flow out of the telescopic tube in one direction. The one-way valve can close the suction head at the end away from the suction tube. A storage chamber for storing the reaction liquid is formed between the fixed plate and the sealing sleeve. A squeezing chamber is formed between the fixed plate and the piston. The injection tube is provided with a strip channel communicating with the storage chamber. A flexible tube is fixedly provided on the sealing sleeve. The outlet chamber is connected to the reaction liquid storage tank through the flexible tube. A solenoid valve is provided on the flexible tube.

4. The supernatant aspiration device for chromosome aberration detection according to claim 3, characterized in that: A connecting sleeve is fixedly installed on the piston. The injection tube is inserted into the connecting sleeve and fixedly connected to the piston. A through hole for the injection tube to pass through is provided in the fixing plate. An elastic sleeve surrounding the injection tube is fixedly installed on the inner wall of the through hole. The elastic sleeve can be slidably fitted onto the connecting sleeve by sliding the fixing plate along the injection tube.

5. The supernatant aspiration device for chromosome aberration detection according to claim 4, characterized in that: The sealing sleeve is fixedly provided with a stop block that cooperates with the strip channel. When the injection tube moves down to the top wall of the strip channel and abuts against the stop block, the injection tube is in the lower limit position.

6. The supernatant aspiration device for chromosome aberration detection according to claim 5, characterized in that: A first cylinder is fixedly installed on the top of the mounting box. The output end of the first cylinder is fixedly connected to a push rod that can extend into the closed sleeve. The first cylinder drives the push rod to move down, which can squeeze the injection tube to move the injection tube down. When the first cylinder pushes the push rod to the maximum stroke, the injection tube is at the lower limit position.

7. The supernatant aspiration device for chromosome aberration detection according to claim 6, characterized in that: The fixing plate is fixedly provided with an upper connecting part and a lower connecting part. The suction tube includes an upper part of the suction tube that is threadedly connected to the upper connecting part and a lower part of the suction tube that is threadedly connected to the lower connecting part. A sealing gasket is provided between the upper part of the suction tube and the lower part of the suction tube and the fixing plate.

8. The supernatant aspiration device for chromosome aberration detection according to claim 7, characterized in that: A retaining ring is fixedly fitted on the injection tube below the strip channel, and the retaining ring is positioned above the fixing plate.

9. The supernatant aspiration device for chromosome aberration detection according to claim 8, characterized in that: A second cylinder is fixedly installed on the top of the mounting box, and the output end of the second cylinder is fixedly connected to the slide plate.

10. The aspiration method of the supernatant aspiration device for chromosomal aberration detection according to any one of claims 1-9, characterized in that, Includes the following steps: S1: Initial state adjustment, the first cylinder outputs thrust to push the push rod to move the injection tube and piston down to the lower limit position, then the first cylinder outputs pull force to disengage the push rod from the injection tube so that the injection tube can slide upward; and the position of the slide plate is adjusted by the second cylinder so that the suction head is above the centrifuge tube; S2: After injecting the test solution into the centrifuge tube and adding the reaction solution for the first centrifugation, place it on the placement plate and add the preset amount of reaction solution to the reaction solution storage tank. S3: Start the second cylinder to drive the slide plate to move down at a constant speed, so that the suction tube moves closer to the centrifuge tube until the baffle moves to the junction of the supernatant and the precipitate. At this time, the elastic sleeve moves down synchronously and is fitted onto the connecting sleeve. When the suction tube moves down, the supernatant is sucked into the suction space by negative pressure, and the solenoid valve is opened so that all the reaction liquid in the reaction liquid storage tank flows into the storage chamber. At this time, the reaction liquid storage tank is set up in an open position. S4: Start the second cylinder to drive the slide plate to move upward at a constant speed and close the solenoid valve so that the suction pipe, the fixed plate and the piston move upward synchronously. At this time, the volume in the suction space remains unchanged, and the injection pipe moves upward along the closed sleeve so that the volume of the storage chamber becomes smaller so that the liquid flows into the injection pipe from the strip channel. Under the action of pressure, the reaction liquid opens the one-way valve so that the reaction liquid forms a jet impact precipitate. S5: Remove the placement plate for secondary centrifugation, and place the collection tank under the suction pipe. Then start the first cylinder to push the push rod to push the injection pipe and piston to squeeze the supernatant in the suction space into the collection tank. S6: Place the centrifuge tubes after secondary centrifugation into the mounting box, and open the solenoid valve to connect the storage chamber with the outside. Then, start the second cylinder to move the suction tube down to draw up the supernatant, and then move the suction tube up to remove the placement plate. Then, start the first cylinder to push the piston to squeeze the supernatant in the suction space into the collection tank. During the downward and upward movement of the suction tube, the storage chamber is connected to the outside so that the one-way valve always closes the telescopic tube.

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