A snp molecular marker combination for identifying or distinguishing grouper germplasm resources and application thereof
By using SNP molecular marker combinations and phylogenetic tree analysis, the problem of inaccurate identification of grouper germplasm resources was solved, enabling rapid and accurate identification and differentiation of germplasm resources.
CN121592785BActive Publication Date: 2026-05-08YAZHOU BAY INNOVATION RESEARCH INSTITUTE HAINAN TROPICAL OCEAN UNIVERSITY
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Patent Information
- Application Number
- CN202610121160.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-29
- Publication Date
- 2026-05-08
- Estimated Expiration
- 2046-01-29
AI Technical Summary
Technical Problem
Existing technologies are insufficient for accurately identifying grouper germplasm resources, especially the golden grouper, tiger grouper, and golden tiger hybrid grouper, leading to inaccurate identification results.
Method used
Using SNP molecular marker combinations, including specific primers and probes, combined with phylogenetic tree analysis via liquid-phase microarrays or kits, we can quickly distinguish between money-patterned, tiger-patterned, and golden-tiger hybrid spots.
Benefits of technology
It enables rapid and accurate identification and differentiation of grouper germplasm resources, improves identification accuracy, and simplifies the operation process.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application belongs to the technical field of molecular biology, and particularly relates to a SNP molecular marker combination for identifying or distinguishing grouper germplasm resources and application thereof. Genomic DNA of golden money grouper, tiger grouper and golden tiger hybrid grouper is extracted, and 177 common SNP sites are obtained after detection, and specific position information is shown in Table 1 of the specification. The SNP molecular marker combination screened by the present application can be developed into primers, probes or gene chips, and through simple phylogenetic tree analysis, golden money grouper, tiger grouper and golden tiger hybrid grouper can be quickly and accurately distinguished, and unknown detection samples can also be subjected to genetic phylogenetic tree analysis with known golden money grouper, tiger grouper and golden tiger hybrid grouper, so that it can be quickly and accurately identified which kind of grouper the unknown detection sample belongs to, and the present application has the characteristics of simple operation, high efficiency, high accuracy and the like, and has important significance in grouper germplasm resource protection and utilization and grouper genetic breeding work.
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