Device and method for detecting deuterium concentration in body through urine

Abstract

The invention discloses a device and method for detecting deuterium concentration in vivo through urine, and the device comprises a urine sample collection unit which is used for collecting a urine sample; the first distillation unit is connected with the urine sample collection unit and is used for carrying out first-round distillation; the second distillation unit is connected with the first distillation unit and is used for carrying out second-round distillation; and the third distillation unit is connected with the second distillation unit and is used for carrying out third-round distillation. The in-vivo deuterium concentration can be detected, the device can be used for detecting the deuterium concentration in urine of a human body and other animals, the device is simple in structure, the method is easy and convenient to operate, safe, reliable and free of requirements for urine samples, the use amount of urine detection is small, treatment and testing can be carried out when the use amount is not smaller than 20 ml, and additional damage to the body is avoided; other body fluid such as blood does not need to be collected, the deuterium concentration in the body can be detected only through urine, and the acceptability and comfort degree of popularization of the method are also improved.

Description

Technical Field

[0001] This invention belongs to the field of isotope detection technology, specifically relating to a device and method for detecting the concentration of deuterium in the body through urine. Background Technology

[0002] Deuterium, also known as heavy hydrogen, is a stable isotope of hydrogen. Its element symbol is still H, but to distinguish it from the more common hydrogen (protium), it is usually written as H₂ or D. A deuterium atom consists of one proton, one neutron, and one electron.

[0003] Deuterium is widely distributed in nature, with an abundance of approximately 0.015% (150 ppm), primarily found in water (especially seawater) and all hydrogen-containing organic matter. Living organisms typically cannot distinguish between hydrogen and deuterium, thus deuterium naturally integrates into biomolecules. The concentration of deuterium in the human body is also around 150 ppm, exceeding the levels of calcium (6 times), magnesium (10 times), potassium (3 times), zinc (90 times), and copper (460 times) in the human body; its concentration is far higher than that of some trace elements in the human body.

[0004] However, high concentrations of deuterium (typically exceeding 20%) are toxic to most organisms because they interfere with delicate biological processes that rely on hydrogen bonds, such as enzyme catalysis and cell division. Advanced manufacturing technologies can remove or reduce deuterium levels; water with a deuterium concentration below 0.015% is called low-deuterium water. Deuterium is a significant carcinogenic factor. Early studies found that reducing deuterium levels in water could enhance the body's antioxidant capacity, prevent disease, delay aging, and activate immune cells. Further research has shown that a 65% reduction in the volume fraction of deuterium in water can inhibit tumor growth. International clinical trials have confirmed that cancer patients consuming low-deuterium water (10-20 ppm) experienced tumor growth halt and significantly improved survival time and quality of life.

[0005] In recent years, in vitro cell culture experiments have shown that low-deuterium water, when used alone, has significant anti-tumor effects on lung cancer cells A549, nasopharyngeal carcinoma cells (CNE-1, CNE-2, 5-8F, 6-10B, Sune-1), prostate cancer cells PC-3, breast cancer cells (MDA, MCF-7), colon cancer cells HT-29, and melanoma cells (M14, HT-199). The National Institute of Hungary reported that low-deuterium water can induce complete or partial regression of spontaneous malignant tumors in cats and dogs, and has applied for registration of low-deuterium water as an anticancer drug for animal use. Studies have found that low-deuterium water reduces the growth rate of the L929 fibroblast cell line and inhibits the growth of xenografts in mice, suggesting that the natural abundance of deuterium can induce and maintain normal cell proliferation.

[0006] Currently, the use of deuterium-rich water as an adjuvant therapy for cancer is also being applied in clinical treatment. Four patients with lung cancer brain metastases, in addition to receiving conventional chemotherapy and radiotherapy, drank deuterium-rich water daily (instead of regular drinking water). The results showed that using deuterium-rich water in combination with conventional clinical treatment could prolong the patients' survival. A phase II double-blind clinical trial using deuterium-rich water in combination with conventional clinical treatment in prostate cancer patients showed that this therapy could reduce prostate volume, lower serum prostate-specific antigen (PSA) concentrations, delay cancer progression, and prolong patient survival. A clinical trial investigating 129 lung cancer patients (including small cell lung cancer, non-small cell lung cancer, lung cancer with brain metastases, and lung cancer without metastases) found that the synergistic effect of deuterium-rich water and conventional therapy could prolong patient survival, with more significant effects in female patients.

[0007] Therefore, monitoring the deuterium concentration in the body can provide clinical evidence for reducing the body's deuterium concentration to assist in disease treatment, and can also provide data support for scientific research on the relationship between deuterium concentration and life activities. However, currently only equipment for detecting deuterium concentration in routine water samples (such as ABB's GLA431 Series Analyzer water isotope analyzer) has high requirements for the TOC, TDS, and other indicators of the samples, and cannot directly detect deuterium concentration in body fluids. Therefore, at present, there are no methods or equipment for detecting deuterium concentration in the body, nor are there any related patent reports. Summary of the Invention

[0008] To address the problems in the prior art, the present invention aims to provide an apparatus and method for detecting the concentration of deuterium in the body through urine.

[0009] To achieve the above objectives and technical effects, the technical solution adopted by this invention is as follows: A device for detecting deuterium concentration in the body via urine, comprising: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

[0010] Furthermore, the urine sample collection unit includes a urine sample bottle and an oil bath. The urine sample bottle is placed in the oil bath, and the urine sample in the urine sample bottle is heated by the oil bath.

[0011] Furthermore, the urine sample is added into the urine sample bottle through a sample inlet funnel or a sample inlet funnel and stop valve I.

[0012] Furthermore, the first distillation unit includes a distillation condenser head I, a collection bottle I, and a high-low temperature integrated machine I. The urine sample bottle is connected to the collection bottle I through the distillation condenser head I. The collection bottle I is placed inside the high-low temperature integrated machine I, and the collection bottle I is filled with an excess of a strong oxidant that does not contain hydrogen.

[0013] Furthermore, the urine sample bottle is connected to the collection bottle I via a distillation condenser head I and a shut-off valve II. The collection bottle I is equipped with a shut-off valve III, which is connected to a vacuum water pump for evacuation and pressure reduction.

[0014] Furthermore, the second distillation unit includes a distillation condenser head II, a collection bottle II, and a high-low temperature integrated machine II. The collection bottle I of the first distillation unit is connected to the collection bottle II through the distillation condenser head II, and the collection bottle II is placed inside the high-low temperature integrated machine II.

[0015] Furthermore, the collecting bottle I is connected to the collecting bottle II via the distillation condenser head II and the shut-off valve IV. The collecting bottle II is equipped with a shut-off valve V, which is connected to a vacuum water pump for evacuation and pressure reduction.

[0016] Furthermore, the third distillation unit includes a distillation condenser head III, a collection bottle III, and a high-low temperature integrated machine III. The collection bottle II of the second distillation unit is connected to the collection bottle III through the distillation condenser head III, and the collection bottle III is placed inside the high-low temperature integrated machine III.

[0017] Furthermore, the collecting bottle II is connected to the collecting bottle III via a distillation condenser head III and a shut-off valve VI. The collecting bottle III is equipped with a shut-off valve VII, which is connected to a vacuum water pump for evacuation and pressure reduction.

[0018] This invention also discloses a method for detecting deuterium concentration in the body through urine, using the device described above for detecting deuterium concentration in the body through urine, comprising the following steps: An excess of a strong oxidizing agent that does not contain hydrogen was placed in collection bottle I; Open stop valve I and add a certain volume of urine sample into the urine sample bottle through the sample addition funnel. Then close stop valve I. Circulate flowing water through the condenser tube of the distillation condenser head I. Open stop valves II and III. Connect stop valve III to a vacuum water pump for depressurization. At the same time, set the temperature of the oil bath to the range of 50℃~75℃ and the temperature of the high and low temperature integrated machine I to the range of 0-5℃ to perform the first round of distillation. Collect the water sample obtained from the distillation into collection bottle I. After the first round of distillation, shut-off valves II and III are closed, and flowing water is circulated into the condenser tube of distillation condenser head II. Shut-off valves IV and V are opened, and shut-off valve V is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine I is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine II is set in the range of 0-5℃ to carry out the second round of distillation. The water sample obtained from the distillation is collected in collection bottle II. After the second round of distillation, shut-off valves IV and V are closed, and flowing water is circulated into the condenser tube of distillation condenser head III. Shut-off valves VI and VII are opened, and shut-off valve VII is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine II is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine III is set in the range of 0-5℃. The third round of distillation is then carried out, and the water sample obtained from the distillation is collected in collection bottle III. After the third round of distillation is completed, shut off valves VI and VII, stop the water circulation in the condenser tube of the distillation condenser, turn off the vacuum and pressure reduction, and restore atmospheric pressure. Finally, the deuterium concentration of the water sample in collection bottle III was measured.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention discloses an apparatus and method for detecting deuterium concentration in the body through urine. It can detect deuterium concentration in the body and can be used not only for humans but also for detecting deuterium concentration in the urine of other animals. The apparatus has a simple structure, the method is easy to operate, safe and reliable, has no requirements for urine samples, and requires only a small amount of urine for processing and testing, no less than 20 ml. It causes no additional damage to the body and does not require the collection of blood or other body fluids. It only requires urine to detect the deuterium concentration in the body, which also improves the acceptability and comfort of the method. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of the present invention. Detailed Implementation

[0021] The present invention will now be described in detail so that its advantages and features can be more easily understood by those skilled in the art, thereby providing a clearer and more explicit definition of the scope of protection of the present invention.

[0022] The following provides a brief overview of one or more aspects to offer a basic understanding of them. This overview is not an exhaustive summary of all conceived aspects, nor is it intended to identify key or decisive elements of all aspects, nor to define the scope of any or all aspects. Its sole purpose is to present some concepts of one or more aspects in a simplified form to prepare for the more detailed descriptions that follow.

[0023] like Figure 1As shown, this invention discloses a device for detecting deuterium concentration in the body through urine, comprising: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

[0024] In some embodiments, the urine sample collection unit includes a sample inlet funnel 1, a stop valve I2, a urine sample bottle 3, and an oil bath 4. The sample inlet funnel 1 is connected to the urine sample bottle 3 through the stop valve I2. The urine sample bottle 3 is placed in the oil bath 4. When the stop valve I2 is opened, the urine sample is added into the urine sample bottle 3 through the sample inlet funnel 1, and the urine sample is heated by the oil bath 4.

[0025] In some embodiments, the first distillation unit includes a distillation condenser head I5, a shut-off valve II6, a collection bottle I7, a high-low temperature integrated machine I8, and a shut-off valve III9. The urine sample bottle 3 is connected to the collection bottle I7 through the distillation condenser head I5 and the shut-off valve II6. The collection bottle I7 is placed inside the high-low temperature integrated machine I8. The collection bottle I7 is filled with an excess of a strong oxidant 20 that does not contain hydrogen. The shut-off valve III9 is ​​connected to a vacuum water pump for evacuation and pressure reduction.

[0026] In some embodiments, the second distillation unit includes a distillation condenser head II10, a shut-off valve IV11, a collection bottle II12, a high-low temperature integrated unit II13, and a shut-off valve V14. The collection bottle I7 is connected to the collection bottle II12 through the distillation condenser head II10 and the shut-off valve IV11. The collection bottle II12 is placed inside the high-low temperature integrated unit II13. The shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction.

[0027] In some embodiments, the third distillation unit includes a distillation condenser head Ⅲ15, a shut-off valve VI16, a collection bottle Ⅲ17, a high-low temperature integrated unit Ⅲ18, and a shut-off valve VII19. The collection bottle Ⅱ12 is connected to the collection bottle Ⅲ17 through the distillation condenser head Ⅲ15 and the shut-off valve VI16. The collection bottle Ⅲ17 is placed inside the high-low temperature integrated unit Ⅲ18. The shut-off valve VII19 is connected to a vacuum water pump for evacuation and pressure reduction.

[0028] This invention also discloses a method for detecting deuterium concentration in the body through urine, comprising the following steps: The implementation process is illustrated using the detection of deuterium concentration in human urine samples as an example. The logic used is also applicable to the detection of deuterium concentration in urine samples from other organisms. An excess of strong oxidant 20, which does not contain hydrogen, is placed in collection bottle I7; Open shut-off valve I2 and add a certain volume of human urine sample into urine sample bottle 3 through sample funnel 1, then close shut-off valve I2. Circulate flowing water through the condenser tube of distillation condenser head I5, open shut-off valves II6 and III9, connect shut-off valve III9 to a vacuum water pump for depressurization, and simultaneously set the temperature of oil bath 4 to the range of 50℃~75℃, and the temperature of high and low temperature integrated machine I8 to the range of 0-5℃ to perform the first round of distillation. The resulting water sample is collected in collection bottle I7. After the first round of distillation, shut-off valves II6 and III9 are closed, and flowing water is circulated into the condenser tube of distillation condenser II10. Shut-off valves IV11 and V14 are opened, and shut-off valve V14 is connected to a vacuum water pump for depressurization. At the same time, the temperature of high and low temperature integrated machine I8 is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine II13 is set in the range of 0-5℃. The second round of distillation is carried out, and the water sample obtained from the distillation is collected in collection bottle II12. After the second round of distillation, shut-off valves IV11 and V14 are closed. Flowing water is circulated into the condenser tube of distillation condenser head III15 to liquefy water vapor into water droplets that fall into the corresponding collection bottle. Shut-off valves VI16 and VII19 are opened. Shut-off valve VII19 is connected to a vacuum water pump for depressurization. At the same time, the temperature of high and low temperature integrated machine II13 is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine III18 is set in the range of 0-5℃. The third round of distillation is carried out, and the water sample obtained from the distillation is collected in collection bottle III17. After the third round of distillation is completed, close the stop valves VI16 and VII19 to stop the water circulation in the condenser tube of the distillation condenser head, turn off the vacuum and pressure reduction, and restore atmospheric pressure. The water sample in collection bottle III17 can be directly tested for deuterium concentration using ABB's GLA431 Series Analyzer water isotope analyzer (without dilution or other operations), thus obtaining the deuterium concentration in the person's urine sample, which also characterizes the deuterium concentration in the person's body.

[0029] The optimal heating temperature in this invention is set within the range of 50℃ to 75℃. In order to accelerate distillation without bumping during vacuum distillation, the heating temperature can be appropriately increased.

[0030] The low-temperature environment in this invention is set within the range of 0~5℃ to reduce the evaporation of liquid water and ensure sufficient collection volume. When the urine sample volume is large and the liquid evaporation rate is slow, the temperature range can be appropriately widened, as long as it ensures that at least 1~2ml of sample can still be obtained after three rounds of distillation, which is sufficient for testing. 0~5℃ is preferred. Conventional methods such as ice-water baths and ice baths can be used, or a refrigeration unit, miniature refrigerator, circulating water chiller, low-temperature incubator, and other equipment capable of achieving this temperature can be used to create the 0~5℃ low-temperature environment.

[0031] In this invention, the ABB GLA431 Series Analyzer water isotope analyzer can be replaced with other equipment for testing the deuterium concentration of clean water samples.

[0032] In some embodiments, the strong oxidant 20, which does not contain hydrogen, can be potassium permanganate, potassium dichromate, persulfate, etc., with potassium permanganate being preferred, to completely oxidize the organic matter remaining in the water sample obtained from the first round of distillation, causing it to denature and decompose.

[0033] In some implementations, the vacuum pump, oil pump, or vacuum pump can be used as the depressurization device. During distillation, depressurization is performed at the opening of the subsequent collection flask to lower the boiling point of water and accelerate the distillation process.

[0034] In some implementations, the urine can be human or from other animals, and there are no requirements regarding the quality of the urine.

[0035] In some implementations, in addition to adding human urine samples into the urine sample bottle 3 through the sample inlet funnel 1 and the stop valve I2, the sample can also be added directly with the cap open, ensuring that the amount of urine sample meets the testing requirements.

[0036] In some implementations, the urine sample volume is 20-100 ml, with the criterion being that at least 1-2 ml can be collected after three rounds of vacuum distillation.

[0037] In some implementations, the oil bath 4 can be replaced with a water bath, sand bath, or any other device that can meet the heating temperature.

[0038] In some implementations, an excess of strong oxidant 20 without hydrogen is added to the collection flask of the first distillation. The strong oxidant 20 is not required for the subsequent two distillations, and the collected sample can be directly subjected to the next distillation.

[0039] In some embodiments, the present invention can be remotely operated and controlled throughout the entire process via a control system such as a PLC or DCS.

[0040] Example 1 like Figure 1 As shown, a device for detecting deuterium concentration in the body via urine includes: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

[0041] The urine sample collection unit includes a sample inlet funnel 1, a stop valve I2, a urine sample bottle 3, and an oil bath 4. The sample inlet funnel 1 is connected to the urine sample bottle 3 through the stop valve I2. The urine sample bottle 3 is placed in the oil bath 4. When the stop valve I2 is opened, the urine sample is added into the urine sample bottle 3 through the sample inlet funnel 1. The urine sample is heated by the oil bath 4.

[0042] The first distillation unit includes a distillation condenser head I5, a shut-off valve II6, a collection bottle I7, a high-low temperature integrated machine I8, and a shut-off valve III9. The urine sample bottle 3 is connected to the collection bottle I7 through the distillation condenser head I5 and the shut-off valve II6. The collection bottle I7 is placed inside the high-low temperature integrated machine I8 and contains an excess of a strong oxidant 20 that does not contain hydrogen. The shut-off valve III9 is ​​connected to a vacuum water pump for evacuation and pressure reduction.

[0043] The second distillation unit includes a distillation condenser head II10, a shut-off valve IV11, a collection bottle II12, a high-low temperature integrated unit II13, and a shut-off valve V14. The collection bottle I7 is connected to the collection bottle II12 through the distillation condenser head II10 and the shut-off valve IV11. The collection bottle II12 is placed inside the high-low temperature integrated unit II13. The shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction.

[0044] The third distillation unit includes a distillation condenser head Ⅲ15, a shut-off valve Ⅵ16, a collection bottle Ⅲ17, a high and low temperature integrated unit Ⅲ18, and a shut-off valve Ⅶ19. The collection bottle Ⅱ12 is connected to the collection bottle Ⅲ17 through the distillation condenser head Ⅲ15 and the shut-off valve Ⅵ16. The collection bottle Ⅲ17 is placed inside the high and low temperature integrated unit Ⅲ18. The shut-off valve Ⅶ19 is connected to a vacuum water pump for evacuation and pressure reduction.

[0045] A method for detecting deuterium concentration in the body via urine includes the following steps: Add 2g of excess potassium permanganate to collection bottle I7; Open shut-off valve I2 and add 50ml of urine sample from a person using normal drinking water from the waterworks into urine sample bottle 3 through sample funnel 1. Then close shut-off valve I2. Circulate flowing water through the condenser tube of distillation condenser head I5. Open shut-off valves II6 and III9. Connect shut-off valve III9 to a vacuum water pump for depressurization. At the same time, set the temperature of oil bath 4 to 65℃ and the temperature of high and low temperature integrated machine I8 to 0℃ to perform the first round of distillation. Collect 34ml of water sample obtained from distillation into collection bottle I7. After the first round of distillation, shut-off valves II6 and III9 are closed, and flowing water is circulated into the condenser tube of distillation condenser II10. Shut-off valves IV11 and V14 are opened, and shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine I8 is set to 70℃, and the temperature of high and low temperature integrated machine II13 is set to 0℃. The second round of distillation is carried out, and 27ml of water sample is collected in collection bottle II12. After the second round of distillation, shut-off valves IV11 and V14 are closed. Flowing water is circulated into the condenser tube of distillation condenser head III15 to liquefy water vapor into water droplets that fall into the corresponding collection bottle. Shut-off valves VI16 and VII19 are opened. Shut-off valve VII19 is connected to a vacuum water pump for depressurization. At the same time, the temperature of high and low temperature integrated machine II13 is set to 70℃ and the temperature of high and low temperature integrated machine III18 is set to 0℃. The third round of distillation is carried out, and the water sample obtained from the distillation is collected in a 20ml collection bottle III17. After the third round of distillation is completed, close the stop valves VI16 and VII19 to stop the water circulation in the condenser tube of the distillation condenser head, turn off the vacuum and pressure reduction, and restore atmospheric pressure. A 20ml water sample was collected from bottle III17. One ml of this sample was directly analyzed using an ABB GLA431 Series Analyzer for deuterium concentration (no dilution or other procedures required). This yielded the deuterium concentration in the urine sample, thus characterizing the deuterium concentration in the individual. The measured deuterium concentration in the urine sample was 150.2 ppm.

[0046] Example 2 like Figure 1 As shown, a device for detecting deuterium concentration in the body via urine includes: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

[0047] The urine sample collection unit includes a sample inlet funnel 1, a stop valve I2, a urine sample bottle 3, and an oil bath 4. The sample inlet funnel 1 is connected to the urine sample bottle 3 through the stop valve I2. The urine sample bottle 3 is placed in the oil bath 4. When the stop valve I2 is opened, the urine sample is added into the urine sample bottle 3 through the sample inlet funnel 1. The urine sample is heated by the oil bath 4.

[0048] The first distillation unit includes a distillation condenser head I5, a shut-off valve II6, a collection bottle I7, a high-low temperature integrated machine I8, and a shut-off valve III9. The urine sample bottle 3 is connected to the collection bottle I7 through the distillation condenser head I5 and the shut-off valve II6. The collection bottle I7 is placed inside the high-low temperature integrated machine I8 and contains an excess of a strong oxidant 20 that does not contain hydrogen. The shut-off valve III9 is ​​connected to a vacuum water pump for evacuation and pressure reduction.

[0049] The second distillation unit includes a distillation condenser head II10, a shut-off valve IV11, a collection bottle II12, a high-low temperature integrated unit II13, and a shut-off valve V14. The collection bottle I7 is connected to the collection bottle II12 through the distillation condenser head II10 and the shut-off valve IV11. The collection bottle II12 is placed inside the high-low temperature integrated unit II13. The shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction.

[0050] The third distillation unit includes a distillation condenser head Ⅲ15, a shut-off valve Ⅵ16, a collection bottle Ⅲ17, a high and low temperature integrated unit Ⅲ18, and a shut-off valve Ⅶ19. The collection bottle Ⅱ12 is connected to the collection bottle Ⅲ17 through the distillation condenser head Ⅲ15 and the shut-off valve Ⅵ16. The collection bottle Ⅲ17 is placed inside the high and low temperature integrated unit Ⅲ18. The shut-off valve Ⅶ19 is connected to a vacuum water pump for evacuation and pressure reduction.

[0051] A method for detecting deuterium concentration in the body via urine includes the following steps: Add 3g of excess potassium permanganate to collection bottle I7; Open shut-off valve I2 and add 100ml of urine sample from someone who has been drinking 115ppm low-deuterium water for 3 years into urine sample bottle 3 through sample funnel 1. Then close shut-off valve I2. Circulate flowing water through the condenser tube of distillation condenser head I5. Open shut-off valves II6 and III9. Connect shut-off valve III9 to a vacuum water pump for depressurization. At the same time, set the temperature of oil bath 4 to 70℃ and the temperature of high and low temperature integrated machine I8 to 4℃ to perform the first round of distillation. Collect 70ml of water sample obtained from distillation into collection bottle I7. After the first round of distillation, shut-off valves II6 and III9 are closed, and flowing water is circulated into the condenser tube of distillation condenser II10. Shut-off valves IV11 and V14 are opened, and shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine I8 is set to 75℃, and the temperature of high and low temperature integrated machine II13 is set to 4℃ to carry out the second round of distillation. The water sample obtained from the distillation is collected in collection bottle II12. After the second round of distillation, shut-off valves IV11 and V14 are closed. Flowing water is circulated into the condenser tube of distillation condenser head III15 to liquefy water vapor into water droplets that fall into the corresponding collection bottle. Shut-off valves VI16 and VII19 are opened. Shut-off valve VII19 is connected to a vacuum water pump for depressurization. At the same time, the temperature of high and low temperature integrated machine II13 is set to 75℃ and the temperature of high and low temperature integrated machine III18 is set to 4℃ to carry out the third round of distillation. The water sample obtained from the distillation is collected in a 40ml collection bottle III17. After the third round of distillation is completed, close the stop valves VI16 and VII19 to stop the water circulation in the condenser tube of the distillation condenser head, turn off the vacuum and pressure reduction, and restore atmospheric pressure. A 40ml water sample was collected from bottle III17. One ml of this sample was directly analyzed using an ABB GLA431 Series Analyzer for deuterium concentration (no dilution or other procedures required). This yielded the deuterium concentration in the urine sample, thus characterizing the deuterium concentration in the individual. The measured deuterium concentration in the urine sample was 115.5 ppm.

[0052] The rest is the same as in Example 1.

[0053] Example 3 like Figure 1 As shown, a device for detecting deuterium concentration in the body via urine includes: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

[0054] The urine sample collection unit includes a sample inlet funnel 1, a stop valve I2, a urine sample bottle 3, and an oil bath 4. The sample inlet funnel 1 is connected to the urine sample bottle 3 through the stop valve I2. The urine sample bottle 3 is placed in the oil bath 4. When the stop valve I2 is opened, the urine sample is added into the urine sample bottle 3 through the sample inlet funnel 1. The urine sample is heated by the oil bath 4.

[0055] The first distillation unit includes a distillation condenser head I5, a shut-off valve II6, a collection bottle I7, a high-low temperature integrated machine I8, and a shut-off valve III9. The urine sample bottle 3 is connected to the collection bottle I7 through the distillation condenser head I5 and the shut-off valve II6. The collection bottle I7 is placed inside the high-low temperature integrated machine I8 and contains an excess of a strong oxidant 20 that does not contain hydrogen. The shut-off valve III9 is ​​connected to a vacuum water pump for evacuation and pressure reduction.

[0056] The second distillation unit includes a distillation condenser head II10, a shut-off valve IV11, a collection bottle II12, a high-low temperature integrated unit II13, and a shut-off valve V14. The collection bottle I7 is connected to the collection bottle II12 through the distillation condenser head II10 and the shut-off valve IV11. The collection bottle II12 is placed inside the high-low temperature integrated unit II13. The shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction.

[0057] The third distillation unit includes a distillation condenser head Ⅲ15, a shut-off valve Ⅵ16, a collection bottle Ⅲ17, a high and low temperature integrated unit Ⅲ18, and a shut-off valve Ⅶ19. The collection bottle Ⅱ12 is connected to the collection bottle Ⅲ17 through the distillation condenser head Ⅲ15 and the shut-off valve Ⅵ16. The collection bottle Ⅲ17 is placed inside the high and low temperature integrated unit Ⅲ18. The shut-off valve Ⅶ19 is connected to a vacuum water pump for evacuation and pressure reduction.

[0058] A method for detecting deuterium concentration in the body via urine includes the following steps: Add 2g of excess sodium persulfate into collection bottle I7; Open stop valve I2 and add 20 ml of urine sample (collected via a metabolic cage) from rats that have been drinking 115 ppm low-deuterium water for one month into urine sample bottle 3 through sample funnel 1. Then close stop valve I2. Circulate flowing water through the condenser tube of distillation condenser head I5. Open stop valves II6 and III9. Connect stop valve III9 to a vacuum water pump for depressurization. Simultaneously, set the temperature of oil bath 4 to 60℃ and the temperature of high and low temperature integrated machine I8 to 0℃ to perform the first round of distillation. Collect 13 ml of water sample obtained from distillation into collection bottle I7. After the first round of distillation, shut-off valves II6 and III9 are closed, and flowing water is circulated into the condenser tube of distillation condenser II10. Shut-off valves IV11 and V14 are opened, and shut-off valve V14 is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine I8 is set to 60℃, and the temperature of high and low temperature integrated machine II13 is set to 0℃ to carry out the second round of distillation. The water sample obtained from the distillation is collected in collection bottle II12. After the second round of distillation, shut-off valves IV11 and V14 are closed. Flowing water is circulated into the condenser tube of distillation condenser head III15 to liquefy water vapor into water droplets that fall into the corresponding collection bottle. Shut-off valves VI16 and VII19 are opened. Shut-off valve VII19 is connected to a vacuum water pump for depressurization. At the same time, the temperature of high and low temperature integrated machine II13 is set within the range of 60℃, and the temperature of high and low temperature integrated machine III18 is set at 0℃. The third round of distillation is carried out, and the water sample obtained from the distillation is collected in a 5ml collection bottle III17. After the third round of distillation is completed, close the stop valves VI16 and VII19 to stop the water circulation in the condenser tube of the distillation condenser head, turn off the vacuum and pressure reduction, and restore atmospheric pressure. Five ml of water sample was collected from bottle III17. One ml of the sample was directly analyzed using an ABB GLA431 Series Analyzer for deuterium concentration (no dilution or other procedures were required). This yielded the deuterium concentration in the rat's urine sample, thus characterizing the deuterium concentration in the rat's body. The measured deuterium concentration in the urine sample was 126.6 ppm.

[0059] The rest is the same as in Example 1.

[0060] Any parts or structures not specifically described in this invention can be made using existing technologies or products, and will not be elaborated upon here.

[0061] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A device for detecting deuterium concentration in the body via urine, characterized in that, include: Urine collection unit, used to collect urine samples; The first distillation unit, connected to the urine sample collection unit, is used to perform the first round of distillation; The second distillation unit, connected to the first distillation unit, is used for the second round of distillation; The third distillation unit, connected to the second distillation unit, is used for the third round of distillation.

2. The device for detecting deuterium concentration in the body via urine according to claim 1, characterized in that, The urine sample collection unit includes a urine sample bottle and an oil bath. The urine sample bottle is placed in the oil bath, and the urine sample in the urine sample bottle is heated by the oil bath.

3. The device for detecting deuterium concentration in the body via urine according to claim 2, characterized in that, The urine sample is added into the urine sample bottle through a sample inlet funnel or a sample inlet funnel and stop valve I.

4. The device for detecting deuterium concentration in the body via urine according to claim 1, characterized in that, The first distillation unit includes a distillation condenser head I, a collection bottle I, and a high-low temperature integrated machine I. The urine sample bottle is connected to the collection bottle I through the distillation condenser head I. The collection bottle I is placed inside the high-low temperature integrated machine I and contains an excess of a strong oxidant that does not contain hydrogen.

5. The device for detecting deuterium concentration in the body via urine according to claim 4, characterized in that, The urine sample bottle is connected to the collection bottle I via a distillation condenser head I and a shut-off valve II. The collection bottle I is equipped with a shut-off valve III, which is connected to a vacuum water pump for evacuation and pressure reduction.

6. The device for detecting deuterium concentration in the body via urine according to claim 1, characterized in that, The second distillation unit includes a distillation condenser head II, a collection bottle II, and a high-low temperature integrated machine II. The collection bottle I of the first distillation unit is connected to the collection bottle II through the distillation condenser head II, and the collection bottle II is placed inside the high-low temperature integrated machine II.

7. The device for detecting deuterium concentration in the body via urine according to claim 6, characterized in that, The collecting bottle I is connected to the collecting bottle II via the distillation condenser head II and the shut-off valve IV. The collecting bottle II is equipped with a shut-off valve V, which is connected to a vacuum water pump for evacuation and pressure reduction.

8. The device for detecting deuterium concentration in the body via urine according to claim 1, characterized in that, The third distillation unit includes a distillation condenser head III, a collection bottle III, and a high-low temperature integrated machine III. The collection bottle II of the second distillation unit is connected to the collection bottle III through the distillation condenser head III, and the collection bottle III is placed inside the high-low temperature integrated machine III.

9. The device for detecting deuterium concentration in the body via urine according to claim 1, characterized in that, The collecting bottle II is connected to the collecting bottle III via the distillation condenser head III and the shut-off valve VI. The collecting bottle III is equipped with a shut-off valve VII, which is connected to a vacuum water pump for evacuation and pressure reduction.

10. A method for detecting deuterium concentration in the body via urine, characterized in that, The detection of deuterium concentration in the body via urine, as described in any one of claims 1-9, includes the following steps: An excess of a strong oxidizing agent that does not contain hydrogen was placed in collection bottle I; Open stop valve I and add a certain volume of urine sample into the urine sample bottle through the sample addition funnel. Then close stop valve I. Circulate flowing water through the condenser tube of the distillation condenser head I. Open stop valves II and III. Connect stop valve III to a vacuum water pump for depressurization. At the same time, set the temperature of the oil bath to the range of 50℃~75℃ and the temperature of the high and low temperature integrated machine I to the range of 0-5℃ to perform the first round of distillation. Collect the water sample obtained from the distillation into collection bottle I. After the first round of distillation, shut-off valves II and III are closed, and flowing water is circulated into the condenser tube of distillation condenser head II. Shut-off valves IV and V are opened, and shut-off valve V is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine I is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine II is set in the range of 0-5℃ to carry out the second round of distillation. The water sample obtained from the distillation is collected in collection bottle II. After the second round of distillation, shut-off valves IV and V are closed, and flowing water is circulated into the condenser tube of distillation condenser head III. Shut-off valves VI and VII are opened, and shut-off valve VII is connected to a vacuum water pump for evacuation and pressure reduction. At the same time, the temperature of high and low temperature integrated machine II is set in the range of 50℃~75℃, and the temperature of high and low temperature integrated machine III is set in the range of 0-5℃. The third round of distillation is then carried out, and the water sample obtained from the distillation is collected in collection bottle III. After the third round of distillation is completed, shut off valves VI and VII, stop the water circulation in the condenser tube of the distillation condenser, turn off the vacuum and pressure reduction, and restore atmospheric pressure. Finally, the deuterium concentration of the water sample in collection bottle III was measured.

Images