Placenta tissue collection bag and application

Abstract

The invention relates to a placenta tissue collecting bag and application. The placenta tissue collecting bag comprises a protective layer, a one-way exhaust valve, a first sealing strip, a second sealing strip, an antioxidant bag and a contact layer. The collection bag is of a double-layer structure, the leakage risk can be remarkably reduced, biological safety protection is improved, the antioxidant sealing bag and the one-way exhaust valve are additionally arranged, the air content in the collection bag can be greatly reduced, and therefore the oxidation rate of placenta tissue is reduced, and cell activity is kept.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a placental tissue collection bag and its application. Background Technology

[0002] The placenta consists of the amnion and chorionic villi of the fetus and the decidua basalis of the mother. Located between the fetus and mother, the placenta is a vital organ for fetal growth and development, performing functions such as substance exchange, defense, synthesis, and immunity. Because the placenta contains a large number of stem cells with the potential for differentiation and proliferation, these stem cells can be re-inducible to differentiate and treat certain diseases after their onset.

[0003] In the collection and preservation of placenta, amnion, and umbilical cord, as well as stem cell extraction and preservation, the quality of placental sample collection and delivery directly affects tissue and cell culture results, and stem cell viability. After the placenta is detached from the uterus, it contains a large amount of blood, which is mixed with meconium, maternal feces, and amniotic fluid, making it highly susceptible to bacterial contamination. Therefore, the collection process can easily lead to sample contamination or a significant reduction in the viability of the collected cells.

[0004] Currently available tissue collection bags use a traditional single-layer structure, which is prone to damage and leakage, resulting in insufficient safety protection. Furthermore, the commonly used antibacterial agent is vitamin C. While vitamin C has good antibacterial effects, it is easily oxidized in practical applications, significantly reducing its effectiveness and shortening the collection and preservation time, thus diminishing its impact on placental tissue viability.

[0005] Therefore, how to prepare a placental tissue collection bag that has a good antibacterial effect and can maintain cell viability for a long time has become an urgent technical problem to be solved. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a placental tissue collection bag and its application. The collection bag has a double-layer structure, which can significantly reduce the risk of leakage, improve biosafety protection, and reduce the amount of air inside the collection bag by adding an antioxidant seal and a one-way exhaust valve, thereby reducing the oxidation rate of placental tissue and maintaining cell activity.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a placental tissue collection bag, the placental tissue collection bag comprising a protective layer, a one-way vent valve, a first seal, a second seal, an antioxidant bag, and a contact layer.

[0009] This invention employs a double-layer structure, significantly reducing the risk of leakage and enhancing the pressure resistance of the collection bag. The combination of a one-way vent valve and an antioxidant seal allows air to escape from the collection bag, while the antioxidant consumes oxygen in the air, thereby reducing the oxidation rate of placental tissue and maximizing cell viability. A double seal further enhances sealing, preventing accidental opening of the collection bag and reducing the risk of leakage.

[0010] Preferably, the protective layer is connected to the contact layer, the contact layer is inside the placental tissue collection bag, the top of the protective layer includes a first seal and a second seal, an antioxidant bag is provided on the surface of the contact layer, and the one-way exhaust valve is located on the surface of the protective layer and communicates with the contact layer.

[0011] Preferably, 1-10% titanium dioxide is added to the protective layer. For example, it can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%.

[0012] Preferably, the first seal and the second seal are located above the protective layer.

[0013] Preferably, the material of the protective layer includes any one or a combination of at least two of linear low-density polyethylene, tin foil, polyvinyl chloride, or polypropylene.

[0014] Preferably, the antioxidant seal is located on the surface of the contact layer.

[0015] Preferably, the antioxidant is placed inside the antioxidant bag.

[0016] Preferably, the antioxidant includes any one or at least a combination of two of reduced iron powder, sodium chloride, or activated carbon.

[0017] Preferably, the amount of antioxidant added is 5-10 g.

[0018] Preferably, the one-way exhaust valve includes an exhaust valve body, an exhaust valve cover, and an exhaust valve orifice.

[0019] Preferably, a functional additive is added to the contact layer.

[0020] Preferably, the functional additive contains open-ring schisandrin, schisandrin, and potassium sorbate.

[0021] In this invention, the functional additives sprayed into the contact layer, specifically the combination of ring-opening neoschisandric acid, schisandrin, and potassium sorbate, exhibit significant antibacterial effects. Compared to their individual use, this combination demonstrates a significant synergistic effect. Compared to commonly used antibacterial agents in the prior art, the aforementioned antibacterial agent possesses stronger antioxidant effects and higher safety, making it more suitable for preserving samples such as cells and tissues.

[0022] Preferably, the mass ratio of the ring-opening neoschisandolic acid, schisandrin, and potassium sorbate is 1:(0.6-1):(0.4-0.8). The (0.6-1) can be, for example, 0.6, 0.7, 0.8, 0.9, or 1. The (0.4-0.8) can be, for example, 0.4, 0.5, 0.6, 0.7, or 0.8.

[0023] Preferably, the amount of the functional additive is 0.6%-0.8%. The 0.6%-0.8% can be, for example, 0.60%, 0.62%, 0.64%, 0.66%, 0.67%, 0.70%, 0.72%, 0.74%, 0.76%, 0.78%, or 0.80%.

[0024] Preferably, the surface of the contact layer has a micro-textured structure.

[0025] This invention sets the surface of the contact layer to a micro-textured structure, which can increase the contact area with placental tissue by 40% while reducing the peeling force by 60%, significantly improving the anti-adhesion effect.

[0026] Preferably, the surface roughness of the contact layer is 3.2-5 μm. For example, it can be 3.2 μm, 3.4 μm, 3.6 μm, 3.8 μm, 4.0 μm, 4.2 μm, 4.4 μm, 4.6 μm, 4.8 μm, or 5.0 μm, etc.

[0027] Preferably, the contact layer comprises an inner layer, an absorbent core, and an outer layer.

[0028] Preferably, the inner layer is made of non-woven fabric.

[0029] Preferably, the absorbent core comprises fluff pulp and superabsorbent polymer.

[0030] Preferably, the mass ratio of the fluff pulp to the superabsorbent polymer is 1:(3-5).

[0031] Preferably, the outer layer is made of polyethylene film.

[0032] Secondly, the present invention provides the application of the placental tissue collection bag described in the first aspect in the collection of placental tissue.

[0033] Thirdly, the present invention provides a method for collecting placental tissue, wherein the placental tissue is collected and preserved using the placental tissue collection bag described in the first aspect.

[0034] Compared with the prior art, the present invention has at least the following beneficial effects:

[0035] 1. This invention employs a double-layer structure to increase the pressure resistance of the collection bag and prevent leakage. It also incorporates a one-way vent valve and antioxidants to reduce the oxidation rate of placental tissue and maximize cell viability.

[0036] 2. The functional additives added to the contact layer in this invention exhibit significant antibacterial effects. Experimental verification has shown that they can effectively inhibit common bacterial strains such as Escherichia coli, Candida albicans, and Staphylococcus aureus. Compared to existing antibacterial agents, the functional additives of this invention have stronger antioxidant effects and higher safety, making them more suitable for preserving samples such as cells and tissues. Attached Figure Description

[0037] Figure 1 This is a diagram of the external structure of the placental tissue collection bag.

[0038] Figure 2 This is a diagram of the internal structure of a placental tissue collection bag.

[0039] Figure 3 This is a top view of the placental tissue collection bag.

[0040] Figure 4 This is a structural diagram of a one-way exhaust valve.

[0041] Figure 5 This is a diagram of cell morphology.

[0042] Among them, 1-protective layer, 2-one-way exhaust valve, 3-first seal, 4-second seal, 5-antioxidant bag, 6-contact layer, 7-exhaust valve body, 8-exhaust valve cover, 9-valve vent. Detailed Implementation

[0043] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments. However, the following examples are merely simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention is determined by the claims.

[0044] The sources of materials used in the following embodiments:

[0045] Fluff pulp: Shandong Sun Paper Co., Ltd.;

[0046] Superabsorbent polymer (SAP): Wanhua Chemical Group Co., Ltd.

[0047] Example 1

[0048] This embodiment prepares a placental tissue collection bag.

[0049] like Figure 1The external structure of the placental tissue collection bag shown is as follows: a first seal and a second seal are sequentially installed on the top of the placental tissue collection bag. The first seal is made of hot melt adhesive, and the second seal is made of pressure-sensitive adhesive. A one-way vent valve is installed on the surface of the protective layer, which is made of 0.15 mm LLDPE (linear low-density polyethylene) film. 5% titanium dioxide is added to the protective layer.

[0050] like Figure 2 The internal structure of the placental tissue collection bag shown is as follows: the contact layer consists of an inner layer, an absorbent core, and an outer layer. The inner layer is made of 5 mm thick medical-grade pure cotton spunlace nonwoven fabric, and the outer layer is a polyethylene film. The absorbent core is composed of fluff pulp and superabsorbent polymer (SAP) in a 1:4 mass ratio. An antioxidant sealing bag is placed on the surface of the contact layer, containing 7 g of antioxidant reduced iron powder. The surface of the contact layer has a micro-textured structure with a roughness of 4 μm.

[0051] 0.72% functional additives were added to the contact layer, and the mass ratio of the ring-opening neoschisandric acid, schisandrin and potassium sorbate was 1:0.8:0.6.

[0052] like Figure 3 As shown, the protective layer and the contact layer are connected together to form a double-layer structure.

[0053] like Figure 4 As shown, the one-way exhaust valve includes an exhaust valve body, an exhaust valve cover, and an exhaust valve orifice.

[0054] Example 2

[0055] This embodiment prepares a placental tissue collection bag.

[0056] like Figure 1 The external structure of the placental tissue collection bag shown is as follows: a first seal and a second seal are sequentially installed on the top of the placental tissue collection bag. The first seal is made of hot melt adhesive, and the second seal is made of pressure-sensitive adhesive. A one-way vent valve is installed on the surface of the protective layer, which is made of 0.15 mm LLDPE (linear low-density polyethylene) film. 1% titanium dioxide is added to the protective layer.

[0057] like Figure 2 The internal structure of the placental tissue collection bag shown is as follows: the contact layer consists of an inner layer, an absorbent core, and an outer layer. The inner layer is made of 5 mm thick medical-grade pure cotton spunlace nonwoven fabric, and the outer layer is a polyethylene film. The absorbent core is composed of fluff pulp and superabsorbent polymer (SAP) in a 1:3 mass ratio. An antioxidant seal is placed on the surface of the contact layer, containing 10 g of sodium chloride antioxidant. The surface of the contact layer has a micro-textured structure with a roughness of 3.2 μm.

[0058] 0.8% functional additives were added to the contact layer, and the mass ratio of the ring-opening neoschisandric acid, schisandrin and potassium sorbate was 1:1:0.4.

[0059] like Figure 3 As shown, the protective layer and the contact layer are connected together to form a double-layer structure.

[0060] like Figure 4 As shown, the one-way exhaust valve includes an exhaust valve body, an exhaust valve cover, and an exhaust valve orifice.

[0061] Example 3

[0062] This embodiment prepares a placental tissue collection bag.

[0063] like Figure 1 The external structure of the placental tissue collection bag shown is as follows: a first seal and a second seal are sequentially installed on the top of the placental tissue collection bag. The first seal is made of hot melt adhesive, and the second seal is made of pressure-sensitive adhesive. A one-way vent valve is installed on the surface of the protective layer, which is made of 0.15 mm LLDPE (linear low-density polyethylene) film. 10% titanium dioxide is added to the protective layer.

[0064] like Figure 2 The internal structure of the placental tissue collection bag shown is as follows: the contact layer consists of an inner layer, an absorbent core, and an outer layer. The inner layer is made of 5 mm thick medical-grade pure cotton spunlace nonwoven fabric, and the outer layer is a polyethylene film. The absorbent core is composed of fluff pulp and superabsorbent polymer (SAP) in a 1:5 mass ratio. An antioxidant sealing bag is placed on the surface of the contact layer, containing 5 g of activated carbon as an antioxidant. The surface of the contact layer has a micro-textured structure with a roughness of 5 μm.

[0065] 0.6% functional additives were added to the contact layer, and the mass ratio of the ring-opening neoschisandric acid, schisandrin and potassium sorbate was 1:0.6:0.8.

[0066] like Figure 3 As shown, the protective layer and the contact layer are connected together to form a double-layer structure.

[0067] like Figure 4 As shown, the one-way exhaust valve includes an exhaust valve body, an exhaust valve cover, and an exhaust valve orifice.

[0068] Example 4

[0069] This embodiment prepares a placental tissue collection bag, which differs from Example 1 only in that the functional additive is potassium sorbate, while the rest is the same as Example 1.

[0070] Example 5

[0071] This embodiment prepares a placental tissue collection bag, which differs from Example 1 only in that schisandrin is not added to the functional additives, and the weight of schisandrin is allocated to potassium sorbate and cyclic schisandric acid according to the proportion in Example 1. The rest is the same as in Example 1.

[0072] Example 6

[0073] This embodiment prepares a placental tissue collection bag, which differs from Example 1 only in that the mass ratio of the open-ring schisandrin, schisandrin and potassium sorbate is 1:0.4:1.2, and all other aspects are the same as in Example 1.

[0074] Example 7

[0075] This embodiment prepares a placental tissue collection bag, which differs from Example 1 only in that the amount of antioxidant added is 3 g, and all other aspects are the same as in Example 1.

[0076] Example 8

[0077] This embodiment prepares a placental tissue collection bag, which differs from Example 1 only in that the surface roughness of the contact layer is 2 μm, and all other aspects are the same as in Example 1.

[0078] Comparative Example 1

[0079] Comparative Example 1 prepared a placental tissue collection bag, which differed from Example 1 only in that the placental tissue collection bag did not include a one-way exhaust valve, and was otherwise identical to Example 1.

[0080] Comparative Example 2

[0081] Comparative Example 1 prepared a placental tissue collection bag, which differed from Example 1 only in that the placental tissue collection bag did not include an antioxidant bag, and was otherwise identical to Example 1.

[0082] Test Example 1

[0083] This test case investigates the viability of cells preserved in placental tissue collection bags.

[0084] Placental specimens were collected using the placental tissue collection bags prepared in the above-described examples and comparative examples, and stored at 2°C for 72 h.

[0085] Mesenchymal stem cells were prepared using the tissue block adherence method. Placental specimens were collected, and the umbilical cord attached to the placenta was cut off, washed with physiological saline until bloodless, cut into small segments, and placed in an empty culture dish. The umbilical cord was torn open with tissue forceps, and the two arteries were dissected. The Walton's jelly distributed around the blood vessels was then removed. The separated Walton's jelly was placed in a 50 mL centrifuge tube, and 2 mL of serum-free mesenchymal stem cell culture medium (LONZA 12-725F) was added.

[0086] Cut the Walton glue into 1mm pieces with scissors. 3 Divide the tissue into small pieces, add 10 mL of a prepared 0.1%-0.2% collagenase II solution, seal the container, and place it in a 37°C water bath with a shaker. Digest at a low speed (150 rpm) for 5 hours. Digest until the tissue pieces become very sparse and the solution becomes viscous, indicating that the collagen has been fully degraded. Add an equal volume of complete culture medium (the serum in which can inhibit collagenase activity) to the digestion solution, and gently pipette the remaining tissue pieces repeatedly to fully release the cells. Filter the cell suspension through a 100-mesh sterile cell sieve to remove incompletely digested tissue pieces and fragments. Collect the filtrate into centrifuge tubes. Resuspend the cell suspension in PBS, centrifuge and wash twice to thoroughly remove residual enzymes. Take a small amount of cell suspension and perform cell counting and viability testing using trypan blue staining. Based on the counting results, cell cells are counted and viability tested at a certain density (1×10⁻⁶). 5 Cell suspension (cells / cm²) was seeded into T25 cell culture flasks. The flasks were then incubated statically at 37°C, 5% CO2, and saturated humidity. Cell images were taken after one week of culture. Details are shown in Table 1.

[0087] Table 1

[0088]

[0089] As can be seen from the above results, as shown in Table 1, the cells preserved using the method of Example 1 have strong activity and a large number of surviving cells.

[0090] Test Example 2

[0091] This test case explores the antibacterial effects of different functional additives.

[0092] This test case included four experimental groups: the functional additives prepared in Examples 1, 4, 5, and 6, and a control group. The aim was to investigate the antibacterial effects of these functional additives against Candida albicans, Escherichia coli, and Staphylococcus aureus.

[0093] (1) Candida albicans

[0094] Candida albicans (ATCC 10231, purchased from Microbiologics KWIK-STIK series) was inoculated onto Salmonella dextrose agar plates according to the instructions and cultured at 25°C for 5 days. Candida albicans were then selected and inoculated onto slant cultures for enrichment. The 24-hour slant culture of the test bacteria was washed with PBS to prepare a bacterial suspension (the required concentration is: 100 μL added to 5 mL of sample solution, recovering 6.5 × 10⁻⁶ bacteria). 4(cfu / mL); take four tubes each of the sample solution (5 mL) and the control solution (5 mL).

[0095] Add 100 μL of the above-mentioned functional additive to a sterile test tube, followed by 5 mL of bacterial suspension. Use PBS instead of the functional additive for parallel tests as a negative control. Take the above bacterial suspension and add 100 μL to each sample solution and control solution, mix thoroughly, and start timing. After 2 min, 5 min, 10 min, and 20 min, add 0.5 mL of each sample solution to a test tube containing 5 mL of PBS, mix thoroughly, and dilute appropriately. Then, take 0.5 mL of each of the three dilutions and place them in two petri dishes. Pour 15 mL of nutrient agar medium cooled to 40℃ into each dish, rotate the petri dish to ensure thorough mixing, and invert the plate after the agar has solidified.

[0096] All samples were incubated at 35℃ for 48 h, and the final results were observed. The experiment was repeated three times, and the inhibition rate was calculated using the following formula: Inhibition rate X = (A0 - A1) / A0 × 100%;

[0097] A0 represents the average colony count of the control group, in units of cfu / mL;

[0098] A1 represents the average colony count of the experimental group, in units of cfu / mL.

[0099] Each group was tested three times. The experimental results of the functional additive in Example 1 are shown in Table 2, and all experimental results are shown in Table 3.

[0100] Table 2

[0101]

[0102] Table 3

[0103]

[0104] (ii) Escherichia coli

[0105] Take Escherichia coli (ATCC 8099, purchased from Microbiologics KWIK-STIK series), and inoculate the strain onto tryptone soybean agar plates according to its instructions. Incubate at 35°C for 48 hours, then select the E. coli and inoculate them onto slant cultures for enrichment culture.

[0106] The 24-hour slant culture of the test bacteria was washed with PBS to prepare a bacterial suspension (the required concentration was: 100 μL added to 5 mL of sample solution, with a recovered bacterial count of 6.5 × 10⁻⁶). 4 (cfu / mL); take four tubes each of the sample solution (5 mL) and the control solution (5 mL).

[0107] Add 100 μL of the above-mentioned functional additive to a sterile test tube, followed by 5 mL of bacterial suspension. Use PBS instead of the antibacterial solution for parallel tests as a negative control. Take the above bacterial suspension and add 100 μL to each sample solution and control solution, mix thoroughly, and start timing. After 2 min, 5 min, 10 min, and 20 min, add 0.5 mL of each sample solution to a test tube containing 5 mL of PBS, mix thoroughly, and dilute appropriately. Then, take 0.5 mL of each of the three dilutions and place them in two petri dishes. Pour 15 mL of nutrient agar medium cooled to 40℃ into each dish, rotate the petri dish to ensure thorough mixing, and invert the plate after the agar has solidified.

[0108] All samples were incubated at 35℃ for 48 h, and the final results were observed. The experiment was repeated three times, and the inhibition rate was calculated using the following formula: Inhibition rate X = (A0 - A1) / A0 × 100%;

[0109] A0 represents the average colony count of the control group, in units of cfu / mL;

[0110] A1 represents the average colony count of the experimental group, in units of cfu / mL.

[0111] Each group was tested three times. The experimental results of the functional additive in Example 1 are shown in Table 4, and all experimental results are shown in Table 5.

[0112] Table 4

[0113]

[0114] Table 5

[0115]

[0116] (iii) Staphylococcus aureus

[0117] Staphylococcus aureus (ATCC 6538, purchased from Microbiologics KWIK-STIK series) was inoculated onto tryptone soybean agar plates according to the instructions and cultured at 25°C for 5 days. Candida albicans was then selected and inoculated onto slant cultures for enrichment. The 24-hour slant cultures of the test bacteria were washed with PBS to prepare a bacterial suspension (the required concentration was 6.5 × 10⁴ cfu / mL when 100 μL was added to 5 mL of the sample solution). Four tubes each of the test sample solution (5 mL) and the control sample solution (5 mL) were prepared.

[0118] Add 100 μL of the above-mentioned functional additive to a sterile test tube, followed by 5 mL of bacterial suspension. Use PBS instead of the functional additive for parallel tests as a positive control. Take the above-mentioned bacterial suspension and add 100 μL to each sample solution and control solution, mix thoroughly, and start timing. After 2 min, 5 min, 10 min, and 20 min, add 0.5 mL of each sample solution to a test tube containing 5 mL of PBS, mix thoroughly, and dilute appropriately. Then, take 0.5 mL of each of the three dilutions and place them in two petri dishes. Pour 15 mL of nutrient agar medium cooled to 40℃ into each dish, rotate the petri dish to ensure thorough mixing, and invert the plate after the agar has solidified.

[0119] All samples were incubated at 35℃ for 48 h, and the final results were observed. The experiment was repeated three times, and the inhibition rate was calculated using the following formula: Inhibition rate X = (A0 - A1) / A0 × 100%;

[0120] A0 represents the average colony count of the control group, in units of cfu / mL;

[0121] A1 represents the average colony count of the experimental group, in units of cfu / mL.

[0122] Each group was tested three times. The experimental results of the functional additive in Example 1 are shown in Table 6, and all experimental results are shown in Table 7.

[0123] Table 6

[0124]

[0125] Table 7

[0126]

[0127] Test Example 3

[0128] This embodiment tests the effect of different storage times.

[0129] (1) Organization and grouping

[0130] Under aseptic conditions, the placental tissue was thoroughly rinsed with pre-cooled PBS to remove blood. The placental tissue was then trimmed into regular 2 cm³ pieces of approximately uniform size and weight. The tissue pieces were randomly assigned to the following groups (at least three replicates per group to ensure statistical significance):

[0131] Experimental group: The tissue blocks were placed in the collection bag of Example 1 and sealed as designed.

[0132] Comparative Example 1 (dry preservation): Tissue blocks were placed in ordinary sterile bags.

[0133] Comparative Example 2 (wet preservation): Tissue blocks were placed in sterile bags containing 10 mL of ordinary physiological saline.

[0134] (2) Simulated transportation / storage

[0135] All sample bags from each group were placed in a 4°C refrigerator to simulate standard refrigerated transport and storage conditions.

[0136] (3) Setting time points and sampling

[0137] T0 (0 hours): Detected immediately at the start of storage as a baseline (activity is defined as 100%).

[0138] T1 (24 hours): Simulates short-distance transportation.

[0139] T2 (72 hours / 3 days): Simulates medium- to long-term storage or inter-provincial transportation.

[0140] T3 (120 hours / 5 days): Extreme challenge, verifying the maximum save window.

[0141] At each preset time point, one bag of samples was taken from each group (n=3).

[0142] (4) Tissue digestion and cell extraction

[0143] Remove the tissue block from the bag and rinse with PBS.

[0144] The tissue blocks were cut into small pieces and placed in a solution containing tissue digestive enzymes (collagenase II, 1 mg / mL), and digested in a constant temperature shaking water bath at 37°C for 40 minutes.

[0145] After digestion, the digestion was terminated with serum-containing culture medium, and the cells were passed through 100 μm and 70 μm cell sieves sequentially to obtain single-cell suspensions. The suspensions were then centrifuged, resuspended, and used for cell counting.

[0146] (5) Cell viability and function detection

[0147] Trypan blue rejection test: Total cell yield and cell viability (percentage of live cells) are calculated using a hemocytometer.

[0148] Table 8

[0149]

[0150] The results are shown in Table 8. With prolonged storage time, the cell viability of placental tissue decreased in all groups. However, the cell viability and metabolic activity extracted from the tissue preserved using the collection bag of Example 1 of this invention were significantly higher than those of the comparative groups at each time point.

[0151] Especially after 120 hours of extreme preservation, the cell viability of the Example 1 group remained above 85%, and the cell morphology was as follows: Figure 5 As shown, conventional preservation methods have become significantly ineffective. This fully demonstrates that the placental collection bag of Example 1 of this invention can effectively maintain the cell viability and function of placental tissue, significantly prolong the effective preservation window of the tissue, and provide high-quality raw materials for downstream cell extraction and application, demonstrating outstanding technological advancement and clinical application value.

[0152] In summary, the collection bag provided by this invention has a double-layer structure, which can significantly reduce the risk of leakage, improve biosafety protection, and reduce the air inside the collection bag by adding an antioxidant seal and a one-way exhaust valve, thereby reducing the oxidation rate of placental tissue and maintaining cell activity.

[0153] The applicant declares that the above description is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention fall within the protection and disclosure scope of the present invention.

Claims

1. A placental tissue collection bag, characterized by, The placental tissue collection bag comprises a protective layer, a one-way exhaust valve, a first seal, a second seal, an antioxidant bag and a contact layer.

2. The placental tissue collection bag of claim 1, wherein, The protective layer is connected with the contact layer, the contact layer is inside the placental tissue collection bag, the top of the protective layer comprises the first seal and the second seal, the surface of the contact layer is provided with the antioxidant bag, and the one-way exhaust valve is located on the surface of the protective layer and communicates with the contact layer.

3. The placental tissue collection bag of claim 1 or 2, wherein, 1-10% titanium dioxide is added to the protective layer. Preferably, the material of the protective layer comprises any one or a combination of at least two of linear low-density polyethylene, tin foil material, polyvinyl chloride or polypropylene. Preferably, the antioxidant bag contains an antioxidant.

4. The placental tissue collection bag of any one of claims 1-3, wherein, The antioxidant comprises any one or a combination of at least two of reduced iron powder, sodium chloride or activated carbon. Preferably, the antioxidant is added in an amount of 5-10 g.

5. The placental tissue collection bag of any one of claims 1-4, wherein, The one-way exhaust valve comprises a valve body, a valve cover and a valve hole.

6. The placental tissue collection bag of any one of claims 1-5, wherein, Functional additives are added to the contact layer. Preferably, the functional additives contain open ring new schisandrin acid, schisandrin and potassium sorbate.

7. The placental tissue collection bag of claim 6, wherein, The mass ratio of the open ring new schisandrin acid, schisandrin and potassium sorbate is 1:(0.6-1):(0.4-0.8). Preferably, the functional additives are added in an amount of 0.6%-0.8%.

8. The placental tissue collection bag of any one of claims 1-7, wherein, The surface of the contact layer is a micro concave-convex texture structure. Preferably, the roughness of the surface of the contact layer is 3.2-5 μm. Preferably, the contact layer comprises an inner layer, an absorbent core and an outer layer. Preferably, the material of the inner layer comprises non-woven fabric. Preferably, the absorbent core comprises fluff pulp and high molecular water-absorbing resin. Preferably, the material of the outer layer comprises polyethylene film.

9. Use of the placental tissue collection bag according to any one of claims 1-8 in collecting placental tissue.

10. A method of collecting placental tissue, comprising: The placental tissue collection bag according to any one of claims 1-8 is used to collect and preserve placental tissue.

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