Resuscitator special for cell culture
By designing a cell culture resuscitation device that includes a water bath and a cryopreservation tube movement device, the inconvenience and safety hazards of traditional cell resuscitation have been solved, enabling a safe and efficient cell thawing process, reducing equipment costs and improving cell survival rate.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-16
- Publication Date
- 2026-03-13
AI Technical Summary
Traditional cell resuscitation methods are inconvenient to operate and pose safety risks. Furthermore, existing equipment is costly and can easily damage cells, making it difficult to achieve precise thawing and maintain the 'little ice heart' state.
A cell culture resuscitation device was designed, which includes a water bath, a cryotube movement device, and an electronic control device. Through the complex movement of the cryotube and constant temperature control, the safety and precision of the cell thawing process are achieved.
It improves the safety and operational efficiency of the cell resuscitation process, reduces equipment costs, avoids local overheating damage to cells, and can process multiple frozen cells simultaneously, meeting the thawing requirements of the 'small ice heart' state.
Smart Images
Figure CN121652909A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to a cell culture resuscitator. This resuscitator is suitable for the rapid thawing of cells from cryogenic storage. It belongs to the field of cell culture. Background Technology
[0002] In traditional cell resuscitation procedures, operators wear cryopreservation gloves and goggles, holding cryovials in a 37°C water bath and rapidly shaking them while using a timer to strictly control the thawing time. Goggles are worn to prevent injury from accidental cryovial explosions. Traditional cell resuscitation methods are inconvenient, especially given the safety hazard of operators forgetting to wear goggles. While a single-mode microwave rapid resuscitation device developed by another company addresses safety and ease of operation, its integrated functions are not essential in cell culture settings, significantly increasing costs and reducing practicality. Furthermore, microwave heating can easily cause localized overheating, potentially damaging cell structure and affecting resuscitation effectiveness and cell viability. Additionally, DMSO in the cryopreservation solution is toxic to cells at high temperatures, requiring the cells to be preserved in a "small ice core" state during resuscitation to mitigate toxicity, a goal that microwave heating cannot precisely achieve. Summary of the Invention
[0003] Purpose of the invention: To overcome the shortcomings of the prior art, a cell culture resuscitator was designed.
[0004] Technical Solution: The cell culture resuscitator consists of three core components: a water bath, a cryovial movement mechanism, and an electronic control unit. The cryovial movement mechanism comprises a component holding the cryovials, a moving rod, a motor, and a support frame. The support frame suspends the cryovial holding component, moving rod, and motor above the water bath. The motor drives the moving rod, which in turn moves the cryovial holding component in the water bath, performing complex movements such as circular, non-circular, elliptical, or reciprocating motions, which are then controlled. The water bath provides the constant temperature required for cell resuscitation. The electronic control unit controls the temperature of the water bath and the movement speed and duration of the cryovial movement mechanism.
[0005] Technical Advantages: Compared with the prior art, this invention solves the problem of injury caused by accidental explosion of cell cryopreservation tubes during traditional cell resuscitation and thawing; reduces the workload and intensity of operators in traditional cell resuscitation; allows for the simultaneous resuscitation of multiple cryopreserved cells; because water baths are specialized equipment in cell culture, and there are no commercially available water baths specifically designed for cell culture, and commercially available products are too large, this invention, due to its targeted purpose, features a smaller water bath device, reducing waste of water and electricity; avoids the impact of localized overheating on the resuscitation effect and cell viability of single-mode microwave rapid resuscitation instruments; avoids the technical requirement of microwave heating not achieving a "small ice core" state; and significantly reduces equipment procurement costs compared to single-mode microwave rapid resuscitation instruments. Attached Figure Description
[0006] Figure 1 Schematic diagram of a cell culture resuscitator.
[0007] Figure 2 Schematic diagram of the movement device for cryopreservation tubes.
[0008] Figure 3 A schematic diagram of the components that house cryopreservation tubes. Detailed Implementation
[0009] Example 1: The cell culture resuscitation device proposed in this invention, such as... Figure 1 As shown, the system includes a water bath device 1, a cryovial movement device 2, and an electronic control device 3. The water bath device 1 has the same structure as a water bath, including a water tank, heating tubes, and a shelf above the heating tubes. The shelf can be used to heat the culture medium. The electronic control device 3, in addition to controlling the water bath temperature, also controls the movement speed and duration of the cryovial movement device. The cryovial movement device, as shown... Figure 2 As shown, the device includes a component 1 for holding cryovials, a moving rod 2, a motor 3, and a support frame 4. The moving rod is connected to the motor at its center, with a fixed vertical suspension rod on each side. The component for holding the cryovials is fixed below the rod, designed to move in a circular motion within the water bath. The motor control cable passes through the support frame and enters the body of the water bath device from the side, below the water tank. The support frame consists of two sections: one section is vertically fixed to the side of the water bath device, and the other section suspends the motor. The two sections can rotate from 0 to 90 degrees around the connection point, allowing the suspended motor section to be horizontal or vertical. At 0 degrees, the two sections can be locked together to ensure stability. Cryovials are inserted in the vertical position, and the culture medium can also be heated in the water bath in this position. The component for holding the cryovials, such as… Figure 3As shown, it includes a tube 1 and a telescopic rotating baffle 2. The telescopic rotating baffle can slide along the direction of the vertical suspension rod. Pushing the baffle upward compresses the baffle spring. When it reaches a certain position, it can rotate laterally and lock, opening the direction of the tube opening. The cryopreservation tube can be placed into the tube. Lowering the telescopic rotating baffle can fix the cryopreservation tube. Both the tube and the baffle are hollow, preferably surrounded by wire to reduce their obstruction to the contact between water and the cryopreservation tube.
Claims
1. A cell culture resuscitation device. It consists of three core parts: a water bath device, a cryopreservation tube movement device, and an electronic control device.
2. The cryopreservation tube movement device of the cell culture resuscitator according to claim 1 includes a component for placing cryopreservation tubes, a movement rod, a motor, and a support frame, characterized in that: Complex movements and controls, such as circular, non-circular, elliptical, or reciprocating motions, are achieved by combining an electric motor and a moving rod to place the cryopreservation tubes.
3. The electronic control device for the cell culture resuscitator according to claim 1, characterized in that: It enables constant temperature control of the water bath device and speed and time control of the cryopreservation tube movement device.