Transmission electron microscope intelligent section staining device

Abstract

The application discloses a transmission electron microscope intelligent section dyeing device and relates to the technical field of section dyeing. The device comprises a machine cabin, a guide rail horizontally fixed in the machine cabin, a dyeing tank assembly slidingly arranged on the guide rail, a lever assembly arranged in the machine cabin and used for driving the dyeing tank assembly to horizontally move along the guide rail, a cover assembly matched with the dyeing tank assembly and connected with a negative pressure controller, and a liquid supply assembly used for supplying different kinds of liquid to the dyeing tank assembly, so that automatic dyeing is realized.

Description

Technical Field

[0001] This invention relates to the field of section staining technology, and more specifically to an intelligent section staining device for transmission electron microscopy. Background Technology

[0002] Transmission electron microscopy is an important tool for observing ultrastructures, and the staining process is crucial in the preparation of biological samples. Since biological samples are mainly composed of light elements such as carbon, hydrogen, oxygen, and nitrogen, they have weak scattering ability for electron beams and low image contrast. Therefore, it is usually necessary to use heavy metal (such as uranium, lead, etc.) staining solutions to combine with specific components of the sample to increase the sample's scattering ability for electrons, thereby improving image contrast.

[0003] However, during the staining process, air bubbles are very likely to get stuck on the sample surface or the back of the screen. These stuck air bubbles will block the contact between the dye and the sample, forming blank staining areas that cannot contact the dye, resulting in uneven staining and seriously affecting the final observation effect and the accuracy of experimental data.

[0004] Secondly, in existing technologies, the staining chamber space is often large and fixed. To ensure complete immersion of the sample, a large amount of staining solution is usually required, resulting in a waste of valuable reagents. On the other hand, during the injection process, the liquid is often injected directly into the chamber, and the flow rate is difficult to control precisely. The injected liquid may generate impact force that directly impacts the sample on the grid, which can easily cause the support film on the grid to rupture or the sample to fall off, resulting in sample rejection.

[0005] Furthermore, in existing dyeing equipment, waste liquid is often directly exposed to air during the discharge process. Due to the lack of an effective sealing structure, the waste liquid is highly susceptible to oxidation or chemical reactions upon contact with air. This is especially true for expensive and chemically unstable dye solutions such as uranium acetate and lead citrate; exposure to air can cause them to deteriorate and lose their original chemical purity. This makes it difficult to effectively recycle and reuse these expensive dye solutions.

[0006] Therefore, it is necessary to provide a smart section staining device for transmission electron microscopy to solve the above problems. Summary of the Invention

[0007] To address the above problems, the present invention provides the following technical solution: a smart section staining device for transmission electron microscopy, comprising:

[0008] Cabin;

[0009] Guide rails, which are horizontally fixed inside the machine compartment;

[0010] A dyeing tank assembly, wherein the dyeing tank assembly is slidably disposed on the guide rail;

[0011] A lever assembly, disposed within the machine compartment, is used to drive the dyeing tank assembly to move horizontally along the guide rail;

[0012] A capping assembly, which is adapted to the dyeing tank assembly and is connected to a negative pressure controller;

[0013] A liquid supply assembly for supplying different types of liquids to the dyeing tank assembly.

[0014] Furthermore, preferably, the dyeing tank assembly includes:

[0015] A slide block, which is slidably mounted on the guide rail;

[0016] The movable seat is a ring structure and is mounted on the slide block in a way that allows it to move up and down via a telescopic cylinder;

[0017] A solution cylinder is integrally formed inside the movable seat, and a retaining ring for placing a sample is provided at the upper opening of the solution cylinder;

[0018] A piston is sealed and slidably disposed in the solution cylinder, and a connecting pipe is connected through the middle of the piston, the connecting pipe being connected to the liquid supply assembly.

[0019] Furthermore, as a preferred embodiment, the movable seat is also integrally formed with a ring seat, and a drainage space is formed between the ring seat and the solution cylinder. The movable seat is also provided with a drain pipe that communicates with the drainage space.

[0020] The side of the solution cylinder is provided with multiple drainage channels;

[0021] A one-way valve is provided in the first connecting pipe, which only allows liquid to enter the solution cylinder from the first connecting pipe.

[0022] Furthermore, as a preferred embodiment, the retaining ring has multiple through holes.

[0023] Furthermore, as a preferred embodiment, a temperature control ring is embedded in the ring seat, and a heat-conducting ring is connected between the temperature control ring and the solution cylinder, the heat-conducting ring extending into the inner wall of the solution cylinder.

[0024] Furthermore, preferably, the liquid supply assembly includes:

[0025] The outer cylinder has multiple liquid supply spaces inside.

[0026] A liquid distributor has multiple input terminals that are respectively connected to each of the liquid supply spaces, and its output terminal is connected to a liquid supply pipe. The liquid supply pipe has a flexible section and a rigid section, wherein the rigid section is located in the slide.

[0027] Connecting pipe two is connected to the rigid section and extends out of the slide, and is connected to connecting pipe one through a connecting cylinder.

[0028] Furthermore, as a preferred embodiment, the liquid supply pipe is connected to a recovery pipe near the second connecting pipe below, and the liquid supply pipe is inclined to guide the liquid flow to the recovery pipe.

[0029] Furthermore, preferably, the capping assembly includes:

[0030] A slide rail, which is horizontally fixed in the machine compartment;

[0031] A follower seat is slidably disposed on the slide rail, and a threaded cylinder is fixed on the follower seat;

[0032] A sleeve is vertically positioned and has threads on its outer surface and a cylindrical groove inside. The sleeve is threadedly connected to the threaded cylinder.

[0033] A follower rod is provided, which can slide into the sleeve, and a return spring is provided between the follower rod and the sleeve;

[0034] A suction seat is fixed below the follower rod and is used for threaded connection with the solution cylinder.

[0035] Furthermore, as a preferred embodiment, one side of the suction seat is connected to an air tube, and the other end of the air tube is connected to a connector, which is connected to the negative pressure controller.

[0036] Furthermore, preferably, the lever assembly includes:

[0037] A lever, the top of which is hinged to the machine compartment and the bottom of which is connected to a plate;

[0038] An adjusting rod, which passes through the plate and is positioned on the plate by a nut;

[0039] A hinge rod, which is hinged between the dyeing tank assembly and the adjusting rod;

[0040] A base, which is fixed in the machine compartment and equipped with a vibrator, the output end of which is hinged to the lever.

[0041] Compared with the prior art, the present invention provides an intelligent section staining device for transmission electron microscopy, which has the following beneficial effects:

[0042] In this invention, the driving seat moves the solution cylinder vertically downwards, causing the sample placed on the retaining ring to actively approach the static liquid surface above the piston. This makes the process of liquid enveloping the sample smoother, avoiding rupture or wrinkling of the support membrane due to liquid impact, and significantly improving the sample preparation yield. Furthermore, this method only requires a small amount of liquid to be maintained above the piston to complete immersion, greatly reducing the amount of dye solution used per batch.

[0043] In this invention, physical shaking is used to disrupt the surface tension of the liquid, causing microbubbles to detach. Rapid suction under negative pressure completely eliminates the retention of bubbles on the sample surface or the back of the support mesh. Furthermore, the negative pressure environment forms an effective gas barrier during the draining and replenishment of the dye solution, preventing carbon dioxide from the outside air from entering and reacting with the dye solution. This ensures the dye solution remains in a good reaction state and improves the consistency of dyeing quality.

[0044] In this invention, by embedding a temperature-controlling ring in the ring seat and utilizing a heat-conducting ring extending into the inner wall of the solution cylinder, not only is the temperature of the dye solution regulated, but the heat-conducting ring also seals off the connection between the drainage space and the outside environment. This structural design creates a relatively closed cavity during dye solution discharge, effectively preventing external air from contaminating the waste liquid, preventing oxidation and deterioration of the dye solution during recycling, creating conditions for the recycling and reuse of the dye solution, and further reducing experimental costs. Attached Figure Description

[0045] Figure 1 This is a schematic diagram of the overall structure of an intelligent section staining device for transmission electron microscopy.

[0046] Figure 2 A three-dimensional structural schematic diagram of a smart section staining device for transmission electron microscopy;

[0047] Figure 3 This is a cross-sectional view of the dyeing tank assembly.

[0048] Figure 4 This is a three-dimensional structural diagram of the capping assembly;

[0049] In the diagram: 1. Machine compartment; 2. Guide rail; 3. Dyeing tank assembly; 4. Lever assembly; 5. Liquid supply assembly; 6. Cap assembly; 7. Negative pressure controller; 31. Slide seat; 32. Connecting cylinder; 33. Connecting pipe one; 34. Piston; 35. One-way valve; 36. Movable seat; 37. Telescopic cylinder; 38. Ring seat; 39. Drain pipe; 310. Solution cylinder; 311. Snap ring; 312. Temperature control ring; 313. Heat conduction ring; 314. Through hole; 41. Lever; 42. Adjusting rod; 43. Hinge rod; 44. Base; 51. Outer cylinder; 52. Liquid supply pipe; 53. Recovery pipe; 54. Connecting pipe two; 61. Slide rail; 62. Follower seat; 63. Connector; 64. Air pipe; 65. Suction seat; 66. Follower rod; 67. Sleeve; 68. Threaded cylinder; 69. Thread. Detailed Implementation

[0050] The terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such terms are interchangeable where appropriate; this is merely a way of distinguishing objects with the same attributes in the embodiments of this application. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion, so that a process, method, system, product, or apparatus that comprises a series of elements is not necessarily limited to those elements, but may include other elements not explicitly listed or inherent to those processes, methods, products, or apparatuses.

[0051] Example: In this embodiment of the invention, please refer to... Figures 1-4 A smart section staining device for transmission electron microscopy is provided, comprising:

[0052] Cabin 1;

[0053] Guide rail 2, which is horizontally fixed inside the machine compartment 1;

[0054] Dyeing tank assembly 3, which is slidably disposed on the guide rail 2;

[0055] A lever assembly 4 is disposed inside the machine compartment 1 and is used to drive the dyeing tank assembly 3 to move horizontally along the guide rail 2;

[0056] A capping assembly 6 is adapted to the dyeing tank assembly 3 and is connected to a negative pressure controller 7;

[0057] Liquid supply assembly 5, which is used to supply different types of liquids to the dyeing tank assembly 3.

[0058] During operation, the lever assembly 4 located in the machine compartment 1 drives the dyeing tank assembly 3 to move horizontally back and forth along the guide rail 2. This horizontal movement is not a station switching, but rather a physical shaking action that disrupts the surface tension balance of the liquid, causing tiny bubbles in the liquid to detach and float to the surface.

[0059] Furthermore, the capping assembly 6 is tightly fitted to the staining tank assembly 3. When the negative pressure controller 7 connected to the capping assembly 6 is activated, a negative pressure environment is formed. This negative pressure environment has a dual function: firstly, it quickly extracts the tiny air bubbles that have detached after horizontal shaking from the staining tank assembly 3, eliminating the retention of air bubbles on the sample surface or the back of the carrier mesh, and preventing the formation of blank staining areas that cannot contact the dye solution; secondly, during the process of draining and adding new solution, the negative pressure environment can effectively block the entry of external gases.

[0060] It should be explained that, since the outside air often contains carbon dioxide, and lead dye solution is very easy to react with carbon dioxide to form precipitate, causing the dye solution to become cloudy and fail, this negative pressure isolation mechanism ensures the purity of the reaction environment inside the dyeing tank component 3 and avoids the deterioration of the dye solution.

[0061] In this embodiment, the dyeing tank assembly 3 includes:

[0062] Slide 31, which is slidably disposed on the guide rail 2;

[0063] The movable seat 36 is a ring structure and is movable up and down on the slide 31 via a telescopic cylinder 37;

[0064] Solution cylinder 310 is integrally formed on the inner side of the movable seat 36, and a retaining ring 311 for placing a sample is provided at the upper opening of the solution cylinder 310.

[0065] Piston 34 is sealed and relatively slidably disposed in the solution cylinder 310. A connecting pipe 33 is connected through the middle of the piston 34 and is connected to the liquid supply assembly 5.

[0066] The telescopic cylinder 37 can be any one of a hydraulic telescopic cylinder, a pneumatic cylinder, or an electric push rod.

[0067] Before the staining operation, the sample is placed on the retaining ring 311 at the opening of the solution cylinder 310.

[0068] During the liquid injection phase, the liquid supply assembly 5 supplies liquid into the solution cylinder 310 through the connecting pipe 33 that passes through the middle of the piston 34. At this time, the piston 34 remains relatively stationary, and the liquid accumulates above the piston 34 to form a calm liquid surface.

[0069] After the venting is completed and the liquid surface returns to stillness, the telescopic cylinder 37 drives the movable seat 36 and the solution cylinder 310 integrally formed with it to move vertically downward. Since the piston 34 remains fixed relative to the slide 31 or the connecting pipe 33, as the solution cylinder 310 moves downward, the sample placed on the retaining ring 311 descends and gradually approaches the still liquid surface above the piston until the sample is immersed in the liquid.

[0070] During this process, the liquid itself is not mechanically pushed or disturbed by the piston. It only relies on the sinking of the solution cylinder 310 to wrap the sample. After the staining is completed, the solution cylinder 310 is moved upward by reversing the operation, and the sample can be removed from the liquid surface.

[0071] In other words, by lowering the solution cylinder 310 and immersing the sample in water, the sample actively approaches the still liquid, and the liquid surface remains almost undisturbed. This avoids physical damage to the carrier mesh and support membrane caused by liquid impact, significantly reducing the sample scrap rate. In addition, only a small amount of liquid needs to be maintained above the piston (the liquid level only needs to be high enough to submerge the bottom of the sample after it has been lowered), and contact can be achieved by lowering the solution cylinder 310, which greatly reduces experimental costs and waste liquid disposal pressure.

[0072] Furthermore, the movable seat 36 is also integrally formed with a ring seat 38, and a drainage space is formed between the ring seat 38 and the solution cylinder 310. The movable seat 36 is also provided with a drain pipe 39 that communicates with the drainage space.

[0073] The side of the solution cylinder 310 is provided with multiple drainage channels;

[0074] A one-way valve 35 is provided in the connecting pipe 33, which only allows liquid to enter the solution cylinder 310 from the connecting pipe 33.

[0075] During the staining and liquid injection stage, the telescopic cylinder 37 drives the movable seat 36 and the solution cylinder 310 to a lower position. At this time, the position of the drain channel on the side of the solution cylinder 310 is lower than the piston 34 (or blocked by the side wall of the piston 34), so that the interior of the solution cylinder 310 forms a closed cavity relative to the outside. The liquid supply component 5 injects liquid into the solution cylinder 310 through the connecting pipe 33 and the one-way valve 35. The one-way valve 35 ensures that the liquid can only enter and will not flow back. The liquid accumulates above the piston 34 and submerges the sample.

[0076] When the dyeing process is complete and drainage is required, the telescopic cylinder 37 drives the movable seat 36 to move the solution cylinder 310 vertically upward relative to the fixed piston 34. As the solution cylinder 310 rises, the drainage channel on the side wall of the cylinder also rises. When the drainage channel gradually rises to a position higher than the piston 34, the piston 34 removes its obstruction of the drainage channel. Under the influence of gravity and the support of the piston 34, the liquid originally accumulated inside the solution cylinder 310 quickly overflows through the open drainage channel into the drainage space between the ring seat 38 and the solution cylinder 310. The liquid entering the drainage space eventually collects and is discharged from the device through the drain pipe 39, thereby achieving rapid cleaning of waste liquid.

[0077] Furthermore, the retaining ring 311 has multiple through holes 314.

[0078] Specifically, when the telescopic cylinder 37 drives the solution cylinder 310 downward, gradually immersing the sample located on the retaining ring 311 into the static liquid above the piston 34, the displaced liquid volume forces the liquid level to gradually rise relative to the retaining ring 311 as the sample descends. During this process, the multiple through holes 314 on the retaining ring 311 act as fluid bypasses. They guide the liquid located below or around the sample, allowing it to flow through the through holes 314 to the upper part of the sample.

[0079] Furthermore, a temperature control ring 312 is embedded in the ring seat 38, and a heat-conducting ring 313 is connected between the temperature control ring 312 and the solution cylinder 310, with the heat-conducting ring 313 extending into the inner wall of the solution cylinder 310.

[0080] First, the temperature control ring 312, embedded in the ring seat 38, acts as a heat source or cold source to regulate the temperature according to the dyeing process requirements. Since one end of the heat conduction ring 313 is connected to the temperature control ring 312 and the other end extends directly into the inner wall of the solution cylinder 310, a highly efficient heat conduction path is formed. This allows the heat or cold generated by the temperature control ring 312 to be directly transferred to the liquid inside the solution cylinder 310, thereby maintaining the reaction temperature required for dyeing.

[0081] More importantly, while connecting the solution cylinder 310 and the ring seat 38, the heat-conducting ring 313 also forms a tight fit with the drainage space, creating a sealed space. When liquid overflows from the drainage channel on the side of the solution cylinder 310 into the drainage space between the ring seat 38 and the solution cylinder 310, the sealing effect of the heat-conducting ring 313 prevents outside air from entering the space and contacting the liquid. This is equivalent to temporarily sealing the overflowing liquid (i.e., waste liquid) in a cavity isolated from the outside until it is discharged through the drain pipe 39.

[0082] It should be explained that in transmission electron microscopy staining, some staining solutions have certain recycling and reuse value. In existing technologies, the discharged waste liquid is often directly exposed to the air, making it highly susceptible to reaction and deterioration. This solution effectively blocks external gas contamination of the waste liquid through the physical sealing of the heat-conducting ring 313, ensuring that the discharged liquid maintains a high chemical purity. This provides a foundation for subsequent waste liquid recycling and treatment, and significantly reduces experimental costs.

[0083] In this embodiment, the liquid supply component 5 includes:

[0084] The outer cylinder 51 has multiple liquid supply spaces inside;

[0085] The liquid distributor has multiple input ends that are respectively connected to each of the liquid supply spaces, and its output end is connected to a liquid supply pipe 52. The liquid supply pipe 52 has a flexible section and a rigid section, wherein the rigid section is located in the slide 31.

[0086] Connecting pipe 2 54 is connected to the rigid section and extends out of the slide block 31, and is connected to connecting pipe 1 33 through connecting cylinder 32.

[0087] The liquid supply pipe 52 is connected to a recovery pipe 53 near the second connecting pipe 54. The liquid supply pipe 52 is inclined to guide the liquid flow to the recovery pipe 53.

[0088] When the liquid distributor switches from one liquid to another, there will inevitably be a residual section of the previous liquid in the pipeline. Utilizing the principles of gravity and fluid dynamics, the inclined setting of the liquid supply pipe 52 will guide this part of the liquid to flow to the lower-positioned recovery pipe 53 and discharge it.

[0089] In this embodiment, the capping assembly 6 includes:

[0090] The slide rail 61 is horizontally fixed in the machine compartment 1;

[0091] Follower seat 62, which is slidably disposed on slide rail 61, and threaded cylinder 68 is fixed on follower seat 62;

[0092] Sleeve 67 is vertically arranged and has threads 69 on its outer surface and cylindrical grooves inside. Sleeve 67 is threadedly connected to threaded sleeve 68.

[0093] Follower rod 66, which can slide into the sleeve 67, and a return spring is provided between the follower rod 66 and the sleeve 67;

[0094] A suction seat 65 is fixed below the follower rod 66 and is used for threaded connection with the solution cylinder 310.

[0095] One side of the suction seat 65 is connected to an air tube 64, and the other end of the air tube 64 is connected to a connector 63, which is connected to the negative pressure controller 7.

[0096] In the unconnected state (i.e., when the suction seat 65 is not threadedly connected to the solution cylinder 310), the suction seat 65 and the follower rod 66 tend to fall naturally due to gravity. At this time, the return spring located between the follower rod 66 and the sleeve 67 is in an extended or pre-tightened state, applying an upward elastic support force to the follower rod 66. This elastic force balances the weight of the suction seat 65 and the follower rod 66, limiting the follower rod 66 to a predetermined height within the sleeve 67, effectively preventing the suction seat 65 from moving down arbitrarily or slipping out of the sleeve 67 due to gravity in the unconnected state.

[0097] When a capping operation is required, the sleeve 67 rotates and moves downward under the drive, and the follower rod 66 and the suction seat 65 rotate and move downward accordingly until the suction seat 65 is screwed into the upper opening of the solution cylinder 310 and a threaded fastening connection is achieved.

[0098] Once the connection is established, the follower rod 66 moves synchronously with the solution cylinder 310. When the solution cylinder 310 moves downward, it drives the suction seat 65 to move downward as well, and the follower rod 66 slides downward relative to it within the sleeve 67.

[0099] In this embodiment, the lever assembly 4 includes:

[0100] A lever 41, the top of which is hinged to the machine compartment 1, and the bottom of which is connected to a plate;

[0101] An adjusting rod 42 passes through the plate and is positioned on the plate by a nut.

[0102] Hinged rod 43, which is hinged between the dyeing tank assembly 3 and the adjusting rod 42;

[0103] The base 44 is fixed in the machine compartment 1 and is equipped with a vibrator. The output end of the vibrator is hinged to the lever 41.

[0104] During implementation, the vibrator fixed on the base 44 in the machine compartment 1 is started, and its output end generates a reciprocating driving force. Since the top of the lever 41 is hinged in the machine compartment 1, forming a lever fulcrum, and the output end of the vibrator is hinged to the lever 41, the reciprocating motion of the vibrator drives the lever 41 to swing left and right around its top hinge point.

[0105] The swing of lever 41 causes adjusting rod 42 to move accordingly. The movement of adjusting rod 42 is transmitted to dyeing tank assembly 3 through hinge rod 43, which in turn pushes dyeing tank assembly 3 to move horizontally and linearly on guide rail 2, thereby expelling air bubbles attached to the sample or cylinder wall and completing the degassing process.

[0106] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A smart section staining device for transmission electron microscopy, characterized in that, include: Cabin (1); Guide rail (2), the guide rail (2) is horizontally fixed inside the machine compartment (1); A dyeing tank assembly (3) is slidably disposed on the guide rail (2); A lever assembly (4) is disposed inside the machine compartment (1) and is used to drive the dyeing tank assembly (3) to move horizontally along the guide rail (2); A capping assembly (6) is adapted to the dyeing tank assembly (3) and is connected to a negative pressure controller (7). Liquid supply assembly (5), which is used to supply different types of liquids to the dyeing tank assembly (3); The dyeing tank assembly (3) includes: A slide (31) is slidably mounted on the guide rail (2); The movable seat (36) is a ring structure and is mounted on the slide (31) in a way that allows it to move up and down via a telescopic cylinder (37); Solution cylinder (310), the solution cylinder (310) is integrally formed on the inner side of the movable seat (36), and a retaining ring (311) for placing the sample is provided at the upper opening of the solution cylinder (310). A piston (34) is sealed and relatively slidably disposed in the solution cylinder (310). A connecting pipe (33) is connected through the middle of the piston (34), and the connecting pipe (33) is connected to the liquid supply assembly (5). The movable seat (36) is also integrally formed with a ring seat (38), and a drain space is formed between the ring seat (38) and the solution cylinder (310). The movable seat (36) is also provided with a drain pipe (39) that connects to the drain space. The side of the solution cylinder (310) is provided with multiple drainage channels; A one-way valve (35) is provided in the first connecting pipe (33), which only allows liquid to enter the solution cylinder (310) from the first connecting pipe (33).

2. The intelligent section staining device for transmission electron microscopy according to claim 1, characterized in that, The retaining ring (311) has multiple through holes (314).

3. The intelligent section staining device for transmission electron microscopy according to claim 1, characterized in that, A temperature control ring (312) is embedded in the ring seat (38), and a heat-conducting ring (313) is connected between the temperature control ring (312) and the solution cylinder (310). The heat-conducting ring (313) extends into the inner wall of the solution cylinder (310).

4. The intelligent section staining device for transmission electron microscopy according to claim 1, characterized in that, The liquid supply assembly (5) includes: The outer cylinder (51) has multiple liquid supply spaces inside; A liquid distributor has multiple input terminals that are connected to each of the liquid supply spaces, and its output terminal is connected to a liquid supply pipe (52). The liquid supply pipe (52) has a flexible section and a rigid section, wherein the rigid section is located in the slide (31). Connecting pipe two (54) is connected to the rigid section and extends out of the slide (31), and is connected to connecting pipe one (33) through connecting cylinder (32).

5. The intelligent section staining device for transmission electron microscopy according to claim 4, characterized in that, The liquid supply pipe (52) is connected to a recovery pipe (53) near the second connecting pipe (54) below. The liquid supply pipe (52) is inclined to guide the liquid to flow to the recovery pipe (53).

6. The intelligent section staining device for transmission electron microscopy according to claim 1, characterized in that, The capping assembly (6) includes: A slide rail (61) is horizontally fixed in the machine compartment (1); Follower seat (62), the follower seat (62) is slidably disposed on the slide rail (61), and a threaded cylinder (68) is fixed on the follower seat (62). A sleeve (67) is vertically arranged and has threads (69) on its outer surface and a cylindrical groove inside. The sleeve (67) is threadedly connected to the threaded cylinder (68). Follower rod (66), which can slide into the sleeve (67) and a return spring is provided between the follower rod (66) and the sleeve (67); A suction seat (65) is fixed below the follower rod (66) and is used for threaded connection with the solution cylinder (310).

7. The intelligent section staining device for transmission electron microscopy according to claim 6, characterized in that, One side of the suction seat (65) is connected to an air tube (64), and the other end of the air tube (64) is connected to a connector (63), which is connected to the negative pressure controller (7).

8. The intelligent section staining device for transmission electron microscopy according to claim 1, characterized in that, The lever assembly (4) includes: A lever (41) is hinged at the top to the machine compartment (1) and connected to a plate at the bottom; Adjusting rod (42), the adjusting rod (42) passes through the plate and is positioned on the plate by a nut; A hinge rod (43) is hinged between the dyeing tank assembly (3) and the adjusting rod (42); The base (44) is fixed in the machine compartment (1) and is equipped with a vibrator. The output end of the vibrator is hinged to the lever (41).

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