Animal cell activity rapid detection device

Abstract

The invention relates to the field of cell activity detection, and discloses an animal cell activity rapid detection device which comprises a main body, a detection table is arranged at the upper end of the main body, a dustproof glass cover is arranged at the upper end of the detection table, and a rapid mixing assembly is arranged on one side of the upper end of the detection table. The rapid mixing assembly is used for mixing animal cells and trypan blue solution or other auxiliary detection solutions, a detection assembly is arranged on the side, corresponding to the rapid mixing assembly, of the upper end of the detection table, the detection assembly is used for detecting the activity of the animal cells, and an auxiliary detection structure is arranged on the other side, corresponding to the detection assembly, of the upper end of the detection table. According to the invention, the grid pattern design divides the microscopic view into standardized grids, and the AI image analysis is combined to automatically and randomly select a plurality of grids to count the number of live and dead cells, so that the traditional manual counting is replaced, the detection speed is increased, meanwhile, contrast experiment data can be formed, and the activity of animal cells can be rapidly and accurately detected; and human errors are reduced, and animal cell activity data are rapidly obtained.

Description

Technical Field

[0001] This invention relates to the field of cell viability detection, and more particularly to a rapid detection device for animal cell viability. Background Technology

[0002] The rapid detection device for animal cell viability is a specialized instrument that integrates biosensing, optical, and electrochemical detection technologies with automated analysis modules. By specifically identifying cell viability-related indicators (such as cell membrane integrity, metabolic enzyme activity, ATP content, and fluorescent marker binding), it rapidly captures cell physiological state signals and converts them into quantifiable data. It can accurately determine the survival rate, proliferation status, or activity level of animal cells (including cultured cells, tissue cells, and cells in biological samples) within a short time (usually minutes to several hours), without the need for complex manual operation. It is widely used in cell culture quality monitoring, drug screening, toxicology testing, biomedical research and development, and cell therapy safety assessment. Its core function is to provide efficient, objective, and reproducible cell viability detection results for scientific research and clinical practice, replacing traditional time-consuming manual counting or staining observation methods, and improving detection efficiency and accuracy.

[0003] In existing technologies, when detecting the activity of animal cells, live and dead cells are labeled and distinguished using trypan blue solution or other auxiliary detection solutions. Finally, the activity of animal cells is determined by observing the cell samples on the slide under a microscope. However, the cell samples on the slide are formed in sheets, requiring manual counting to distinguish and determine the activity value of animal cells, which is inefficient. Furthermore, in the absence of comparative experimental data, detection errors are prone to occur due to manual detection. When the detection device is placed in daily use, it is directly exposed to the air, which shortens the equipment maintenance cycle. Moreover, the detection modules are directly placed on the detection stage, which is prone to displacement due to operational vibrations, affecting the stability of sample placement. Summary of the Invention

[0004] The technical problem this invention aims to solve is to provide a rapid detection device for animal cell viability. In existing technologies, when detecting animal cell viability, live and dead cells are labeled and distinguished using trypan blue solution or other auxiliary detection solutions. Finally, the viability of the animal cells is determined by observing the cell samples on a microscope slide. However, the cell samples on the slide are often in large slabs, requiring manual counting and differentiation to determine the viability value, which is inefficient. Furthermore, manual detection without comparative experimental data is prone to errors. Additionally, the detection device is directly exposed to air during routine use, shortening the equipment maintenance cycle. Moreover, the detection modules are directly mounted on the detection stage, making them susceptible to displacement due to operational vibrations, affecting the stability of sample placement.

[0005] To address the aforementioned problems, the present invention is implemented through the following technical solution.

[0006] A rapid detection device for animal cell viability includes: a main body, a detection stage at the upper end of the main body, and a dustproof glass cover at the upper end of the detection stage; a rapid mixing component on one side of the upper end of the detection stage, the rapid mixing component being used to mix animal cells with trypan blue solution or other auxiliary detection solutions; a detection component on the upper end of the detection stage next to the rapid mixing component, the detection component being used to detect animal cell viability; an auxiliary detection structure on the upper end of the detection stage on the other side corresponding to the detection component; and a main magnetic plate embedded in the middle of the upper end of the detection stage.

[0007] In one embodiment, the dustproof glass cover is a hollow rectangular structure, and the portion of the testing platform that contacts the bottom of the dustproof glass cover has a reserved slot.

[0008] In one embodiment, the dustproof glass cover has symmetrical hand-held slots on both sides of its exterior, and the bottom of the dustproof glass cover that contacts the testing platform is glued and fixed with a rubber pad.

[0009] In one embodiment, the rapid mixing assembly includes a first base, a motor, a rotating shaft, connecting blades, an outer rotating cylinder, an inner groove, an anti-slip pad, a test tube, and a sealing plug. The motor is located at the center of the upper end of the first base, and the driving end of the motor is provided with a rotating shaft.

[0010] In one embodiment, the outer ring of the rotating shaft is provided with connecting blades at equal intervals in a circular shape, and the side of the connecting blades away from the rotating shaft is bonded together with an outer rotating cylinder. The inner side of the outer rotating cylinder is provided with embedded grooves at equal intervals in a circular shape, and anti-slip pads are provided in the embedded grooves.

[0011] In one embodiment, the anti-slip pad is bonded and fixed to the outer rotating cylinder, and a test tube is placed inside the anti-slip pad, with a sealing plug at the open end of the test tube, and there are no fewer than three test tubes.

[0012] In one embodiment, the detection assembly includes a second base, a mounting slot, a glass slide, a grid pattern, an electron microscope, a data processor, and a display screen. The mounting slot is provided on one side of the upper end of the second base, and a glass slide is placed in the mounting slot. An electron microscope is provided on the side of the upper end of the second base corresponding to the mounting slot. A data processor is provided in the longitudinal center of the electron microscope, and a display screen is tilted at the upper end of the electron microscope.

[0013] In one embodiment, the glass slide is made of high-transparency glass, and a grid pattern is provided horizontally in the middle of the glass slide, the grid pattern being composed of multiple squares of the same area.

[0014] In one embodiment, the auxiliary detection structure includes a third base, a groove, a connecting magnetic plate, a mounting frame, a socket, and an operating tube. The upper end of the third base is provided with a mounting frame, and the mounting frame has horizontally equidistant sockets, and an operating tube is placed in each socket. There are at least two operating tubes.

[0015] In one embodiment, grooves are provided on both sides of the third base, the first base and the second base, and a connecting magnetic plate is embedded in the bottom of the third base, the first base and the second base.

[0016] This invention provides a rapid detection device for animal cell viability. Compared with the prior art, it has the following advantages:

[0017] The grid pattern design divides the microscopic field of view into standardized squares. Combined with AI image analysis, it automatically and randomly selects several squares to count the number of live and dead animal cells, replacing traditional manual counting. This improves the detection speed and allows for the generation of comparative experimental data, enabling rapid and accurate detection of animal cell activity while reducing human error and quickly obtaining animal cell activity data. The magnetic connection structure (main magnetic plate and connecting magnetic plate) allows for quick assembly and disassembly, ensuring that each component is stably positioned and avoiding displacement caused by operational vibrations. It also supports flexible movement of different detection modules, and each module can be stably positioned during experiments. The hollow dustproof glass cover, combined with rubber gasket sealing and reserved slots, forms an efficient physical barrier to block environmental pollutants, ensure sample purity, and extend the equipment maintenance cycle. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the rapid detection device for animal cell activity.

[0019] Figure 2 A schematic diagram of the structure of the rapid detection station for animal cell viability after removing the dustproof glass cover.

[0020] Figure 3 This is a schematic diagram of the structure of the rapid animal cell activity detection device after the detection stage and dustproof glass cover are separated.

[0021] Figure 4 A schematic diagram of the auxiliary detection structure for a rapid detection device for animal cell viability.

[0022] Figure 5 This is a schematic diagram of the detection component structure of a rapid detection device for animal cell viability.

[0023] Figure 6 This is a schematic diagram of the rapid mixing component structure of a rapid animal cell activity detection device.

[0024] Figure 7 This is a schematic diagram of the slide structure of a rapid detection device for animal cell viability.

[0025] The attached figures are labeled as follows:

[0026] 1. Main body; 2. Testing stage; 3. Dustproof glass cover; 4. Reserved slot; 5. Handheld slot; 6. Rubber pad; 7. Rapid mixing component; 701. First base; 702. Motor; 703. Rotating shaft; 704. Connecting blade; 705. Outer rotating cylinder; 706. Embedded groove; 707. Anti-slip pad; 708. Test tube; 709. Sealing plug; 8. Testing component; 801. Second base; 802. Placement slot; 803. Glass slide; 804. Grid pattern; 805. Electron microscope; 806. Data processor; 807. Display screen; 9. Auxiliary testing structure; 901. Third base; 902. Groove; 903. Connecting magnetic plate; 904. Placement frame; 905. Insertion hole; 906. Operating tube; 10. Main magnetic plate. Detailed Implementation

[0027] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of protection of the present invention.

[0028] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0029] Reference Figures 1-7 A rapid detection device for animal cell viability includes: a main body 1, a detection platform 2 at the upper end of the main body 1, and a dustproof glass cover 3 at the upper end of the detection platform 2; a rapid mixing component 7 on one side of the upper end of the detection platform 2, which is used to mix animal cells with trypan blue solution or other auxiliary detection solutions; a detection component 8 on the side of the upper end of the detection platform 2 corresponding to the rapid mixing component 7, which is used to detect animal cell viability; an auxiliary detection structure 9 on the other side of the upper end of the detection platform 2 corresponding to the detection component 8; and a main magnetic plate 10 embedded in the middle of the upper end of the detection platform 2.

[0030] The dustproof glass cover 3 has a hollow rectangular structure, and the part of the test table 2 that contacts the bottom of the dustproof glass cover 3 has a reserved slot 4.

[0031] The dustproof glass cover 3 has symmetrical hand-held slots 5 on both sides of its exterior, and the bottom part of the dustproof glass cover 3 that contacts the testing table 2 is glued and fixed with a rubber pad 6.

[0032] The rapid mixing assembly 7 includes a first base 701, a motor 702, a rotating shaft 703, a connecting blade 704, an outer rotating cylinder 705, an inner groove 706, an anti-slip pad 707, a test tube 708, and a sealing plug 709. The motor 702 is located at the center of the upper end of the first base 701, and the rotating shaft 703 is located at the drive end of the motor 702.

[0033] The outer ring of the rotating shaft 703 is circular and equidistantly provided with connecting blades 704. The side of the connecting blades 704 away from the rotating shaft 703 is bonded to an outer rotating cylinder 705. The inner side of the outer rotating cylinder 705 is circular and equidistantly provided with an inner groove 706, and an anti-slip pad 707 is provided in the inner groove 706.

[0034] The anti-slip mat 707 is bonded and fixed to the outer rotating cylinder 705. A test tube 708 is placed inside the anti-slip mat 707, and the open end of the test tube 708 is provided with a sealing plug 709. There are no fewer than three test tubes 708.

[0035] The detection component 8 includes a second base 801, a mounting groove 802, a glass slide 803, a grid pattern 804, an electron microscope 805, a data processor 806, and a display screen 807. The mounting groove 802 is provided on one side of the upper end of the second base 801, and the glass slide 803 is placed in the mounting groove 802. The electron microscope 805 is provided on the side of the upper end of the second base 801 corresponding to the mounting groove 802. The data processor 806 is provided in the longitudinal middle of the electron microscope 805, and the display screen 807 is tilted on the upper end of the electron microscope 805.

[0036] The slide 803 is made of high-transparency glass. A grid pattern 804 is horizontally arranged in the middle of the slide 803. The grid pattern 804 is composed of multiple squares of the same area.

[0037] The auxiliary detection structure 9 includes a third base 901, a groove 902, a connecting magnetic plate 903, a mounting frame 904, a socket 905, and an operating tube 906. The upper end of the third base 901 is provided with a mounting frame 904, and the mounting frame 904 is provided with sockets 905 at equal intervals in the transverse direction. An operating tube 906 is installed in the socket 905, and there are no fewer than two operating tubes 906.

[0038] The third base 901, the first base 701 and the second base 801 are all provided with grooves 902 on both sides, and the bottom of the third base 901, the first base 701 and the second base 801 are all inlaid with connecting magnetic plates 903.

[0039] During use, motor 702 drives rotating shaft 703 to rotate connecting blades 704 and outer rotating cylinder 705 at high speed, creating eddy current disturbance. This ensures that the cell suspension in test tube 708 placed in inner groove 706 is in full contact with trypan blue solution (or other auxiliary detection solutions, using trypan blue solution as an example here), ensuring accurate subsequent experimental data. Live cell membranes remain intact, rejecting trypan blue dye; dead cell membranes are damaged, allowing dye to enter and turn the cells blue. A sealing plug 709 is plugged into the open end of test tube 708. The inner groove 706 of outer rotating cylinder 705 and anti-slip pad 707 fix test tube 708, preventing sample splashing or damage to test tube 708 due to vibration during mixing. The system allows for simultaneous mixing of multiple test tubes 708, improving high-throughput detection efficiency. After the cell suspension and trypan blue solution in test tube 708 are mixed, the sealing plug 709 is opened, and the operating tube 906 placed in the insertion hole 905 of the placement rack 904 is removed. The mixed cell sample to be tested is aspirated from test tube 708 and dripped onto the glass slide 803 placed in the placement tank 802. The glass slide 803 holds the stained cell sample, and the electron microscope 805 automatically scans the glass slide 803 to capture high-definition images. The data processor 806 processes the images and projects them onto the display screen 807. The glass slide 803 contains multiple square mesh structures of the same area. The grid pattern 804 divides the field of view into equal-area squares. The display screen 807 shows the grid pattern 804 and the animal cells separated by the grid pattern 804. Combined with AI image analysis, it automatically and randomly selects several squares to count the number of live and dead cells, replacing traditional manual counting. This improves the detection speed and allows for the generation of comparative experimental data, enabling rapid and accurate detection of animal cell activity and reducing human error. Connecting magnetic plates 903 are embedded in the bottom of the third base 901, the first base 701, and the second base 801, and a main magnetic plate 10 is embedded in the upper center of the detection stage 2. Both the 701 and the second base 801 can be stably placed with the main magnetic plate 10 by magnetic attraction through the connecting magnetic plate 903 at the bottom, making them difficult to move. The third base 901, the first base 701, and the second base 801 all have grooves 902 on both sides, which facilitates lifting the auxiliary detection structure 9, the rapid mixing component 7, and the detection component 8 respectively. When placing the main body 1 and the detection table 2, place both hands in the hand-held slots 5 and align the rubber pad 6 at the bottom of the dustproof glass cover 3 with the reserved slot 4 and insert it. At this time, the dustproof glass cover 3 can prevent dust from falling onto the auxiliary detection structure 9, the rapid mixing component 7, and the detection component 8, thus achieving a dustproof effect and ensuring that the experiment can be carried out at any time.

[0040] Therefore, although the invention has been described herein with reference to specific embodiments thereof, freedom of modification, various changes and substitutions are also within the scope of the foregoing disclosure, and it should be understood that in some cases, certain features of the invention may be adopted without departing from the scope and spirit of the invention and without corresponding use of other features. Thus, many modifications can be made to adapt a particular environment or material to the essential scope and spirit of the invention. The invention is not intended to be limited to the specific terminology used in the following claims and / or the specific embodiments disclosed as the best mode for carrying out the invention, but the invention will include any and all embodiments and equivalents falling within the scope of the appended claims. Therefore, the scope of the invention will be defined only by the appended claims.

Claims

1. A device for rapid detection of animal cell activity, characterized by Include: The main body (1), the upper end of the main body (1) is provided with a detection table (2), and the upper end of the detection table (2) is provided with a dustproof glass cover (3), one side of the upper end of the detection table (2) is provided with a rapid mixing assembly (7), the rapid mixing assembly (7) is used for mixing animal cells and trypan blue solution or other auxiliary detection solution, the upper end of the detection table (2) is provided with a detection assembly (8) corresponding to the side of the rapid mixing assembly (7), the detection assembly (8) is used for detecting the activity of animal cells, the upper end of the detection table (2) is provided with an auxiliary detection structure (9) corresponding to the other side of the detection assembly (8), and the middle part of the upper end of the detection table (2) is inlaid with a main magnetic plate (10).

2. The rapid detection device for animal cell activity according to claim 1, wherein The dustproof glass cover (3) is a hollow rectangular structure, and the part of the detection table (2) contacting the bottom of the dustproof glass cover (3) is provided with a reserved clamping groove (4).

3. The rapid animal cell activity detection device according to claim 1, wherein The dustproof glass cover (3) is symmetrically provided with a hand-held groove (5) on the outside, and the part of the dustproof glass cover (3) contacting the detection table (2) is adhesively fixed with a rubber pad (6).

4. The rapid animal cell activity detection device according to claim 1, wherein The rapid mixing assembly (7) comprises a first base (701), a motor (702), a rotating shaft (703), a connecting blade (704), an outer rotating cylinder (705), an embedded groove (706), an anti-skid pad (707), a test tube (708) and a sealing plug (709), and the first base (701) is provided with the motor (702) at the center of the upper end, and the driving end of the motor (702) is provided with the rotating shaft (703).

5. The rapid detection device for animal cell activity according to claim 4, wherein The connecting blades (704) are circularly and equidistantly arranged on the outer ring of the rotating shaft (703), and the connecting blades (704) are adhesively connected with the outer rotating cylinder (705) away from the rotating shaft (703). The inner part of the outer rotating cylinder (705) is circularly and equidistantly provided with the embedded groove (706), and the embedded groove (706) is provided with the anti-skid pad (707).

6. The rapid animal cell activity detection device according to claim 5, wherein The anti-skid pad (707) is adhesively fixed with the outer rotating cylinder (705), the test tube (708) is arranged in the inner side of the anti-skid pad (707), and the opening end of the test tube (708) is provided with the sealing plug (709). The test tube (708) is not less than three.

7. The rapid animal cell activity detection device according to claim 1, wherein The detection assembly (8) comprises a second base (801), a mounting groove (802), a glass slide (803), a grid pattern (804), an electron microscope (805), a data processor (806) and a display screen (807), the upper end of the second base (801) is provided with the mounting groove (802) on one side, and the mounting groove (802) is provided with the glass slide (803), the upper end of the second base (801) is provided with the electron microscope (805) corresponding to the side of the mounting groove (802), the longitudinal middle part of the electron microscope (805) is provided with the data processor (806), and the upper end of the electron microscope (805) is provided with the display screen (807).

8. The rapid animal cell activity detection device according to claim 7, wherein The glass slide (803) is made of high-transparency glass material, and the middle part of the glass slide (803) is transversely provided with the grid pattern (804), and the grid pattern (804) is composed of a plurality of square shapes with the same area.

9. The rapid animal cell activity detection device according to claim 1, wherein The auxiliary detection structure (9) comprises a third base (901), a groove (902), a connecting magnetic plate (903), a mounting rack (904), a jack (905) and an operating tube (906), the upper end of the third base (901) is provided with the mounting rack (904), jack (905) is horizontally and equidistantly arranged in the mounting rack (904), and the operating tube (906) is arranged in the jack (905), and the operating tube (906) is not less than two.

10. The rapid animal cell activity detection device according to claim 9, wherein The two sides of the third base (901), the first base (701) and the second base (801) are provided with the groove (902), and the bottom of the third base (901), the first base (701) and the second base (801) is inlaid with the connecting magnetic plate (903).

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