Constant-pressure heat cover device for gene amplification instrument

Abstract

The invention relates to a constant-pressure heat cover device for a gene amplification instrument, and the device comprises a heat cover assembly which comprises at least one installation cavity used for placing a reaction tube; the heat cover bracket comprises a first connecting part and a second connecting part, and the first connecting part is movably connected with the heat cover assembly; the rotating piece is rotatably arranged above the mounting cavity and is connected between the two first connecting parts; the moving part is arranged on one side of the rotating part and is movably connected with the second connecting part; the moving part is provided with a first inclined surface, and the first inclined surface is used for abutting against the rotating part; the first elastic piece is arranged between the hot cover bracket and the rotating piece; when the heat cover assembly is pressed on the reaction tube, the reaction tube reversely pushes the rotating part to rotate and move in the first direction, and pushes the moving part to move in the second direction. The floating rotating part is in contact with the reaction tube to drive the moving part with the inclined surface to move; the first elastic piece is compressed to generate constant pressure, dimensional changes caused by tolerance, thermal expansion and the like are absorbed, and the pressure is kept constant.

Description

Technical Field

[0001] This application relates to the field of biotechnology instruments, and in particular to a constant pressure heated cap device for a gene amplification instrument. Background Technology

[0002] With the application of polymerase chain reaction (PCR) technology, the requirements for precise control of the reaction process are becoming increasingly stringent. During PCR, the reaction tubes undergo a high-temperature denaturation step, during which the liquid inside the tube easily evaporates and condenses on the cap, affecting reaction efficiency and result consistency. To address this, heated cap technology has emerged. By maintaining the cap at a temperature higher than the boiling point of the dry sample, it effectively prevents condensation and has become a standard feature of PCR instruments.

[0003] In related technologies, static pressure is provided using rigid springs or screws through fixed pressure or manual pressure adjustment modes. This method is simple in structure and low in cost. However, in practical use, due to the high manufacturing tolerances of PCR reaction tubes from different brands and batches, it is difficult to uniformly adapt the fixed pressure to the differences between tubes. This can easily lead to insufficient pressure causing evaporation, or excessive pressure causing tube cap deformation and leakage. In addition, after long-term use, springs are prone to fatigue, and thermal expansion effects can also affect pressure stability, making it difficult to ensure constant pressure throughout the experiment. Alternatively, manual rotation can be used to achieve overpressure slippage and constant pressure locking. Although it can achieve a certain degree of pressure adjustment, the operation requires multiple forward and reverse rotations, the positions of the constant pressure point and the release point are uncertain, the operation is cumbersome and inconsistent, the user experience is poor, and mechanical bounce can easily occur when releasing, which may damage the instrument structure.

[0004] The aforementioned hot cap device has several problems, including the inability of the fixed pressure hot cap to adapt to the tolerances of the reaction tube, resulting in poor pressure consistency; the hand-rotating mechanism is complex to operate, provides a poor user experience, and still relies on manual intervention. Summary of the Invention

[0005] Therefore, it is necessary to provide a constant pressure hot cap device for gene amplification instruments to address the problems of existing hot cap devices, such as inability to adapt to reaction tube height tolerances, maintain constant pressure, poor ease of operation, and high structural complexity.

[0006] A constant pressure heating cap device for a gene amplification instrument, comprising:

[0007] A heated cap assembly includes at least one mounting cavity for placing a reaction tube;

[0008] The heat cover bracket includes a first connecting part and a second connecting part, wherein the first connecting part is movably connected to the heat cover assembly;

[0009] A rotating component is rotatably disposed above the mounting cavity and connected between the two first connecting portions;

[0010] A moving part is arranged on one side of the rotating part and movably connected with the second connecting part; the moving part is provided with a first inclined surface for abutting against the rotating part;

[0011] A first elastic part is arranged between the hot cover support and the rotating part;

[0012] When the hot cover assembly is pressed against the reaction tube, the reaction tube pushes the rotating part to rotate and move in the first direction, and pushes the moving part to move in the second direction.

[0013] In one of the embodiments, the first connecting part is provided with a sliding groove near one end of the hot cover assembly; the rotating part is a roller, and the roller is connected with the two sliding grooves through a rotating shaft.

[0014] In one of the embodiments, the sliding groove is arranged in the first direction, and the two ends of the rotating shaft are arranged in the sliding groove and move in the first direction.

[0015] In one of the embodiments, the second connecting part is provided with a guide groove near one end of the hot cover assembly, and the guide groove has an included angle with the first direction; the moving part is a guide rod, one end of the guide rod is provided with a wedge, and the first inclined surface is arranged on the side of the wedge close to the roller; the other end of the guide rod is movably connected with the guide groove.

[0016] In one of the embodiments, the second connecting part is provided with the guide groove on both sides, and the guide groove is a strip-shaped groove; the moving part further comprises a guide block, the guide block is installed on one end of the guide rod close to the second connecting part and movably connected with the guide groove through a bolt.

[0017] In one of the embodiments, it further comprises a first mounting plate and a second mounting plate movably connected with each other, the first mounting plate is arranged on the moving part, the second mounting plate is fixedly arranged between the hot cover support and the first mounting plate, and the first mounting plate moves in the first direction.

[0018] In one of the embodiments, the first mounting plate and the second mounting plate are fixed through a plurality of screws; it further comprises a first elastic part, one end of the first elastic part is connected with the second connecting part, and the other end of the first elastic part is connected with the screw.

[0019] In one of the embodiments, it further comprises a second elastic part, one end of the second elastic part is connected with the moving part, and the other end of the second elastic part is connected with the second connecting part; the elastic force of the first elastic part is greater than the elastic force of the second elastic part.

[0020] In one of the embodiments, a plurality of limit posts are further included, one end of the limit post is installed on the first mounting plate, and a gap exists between the other end of the limit post and the second mounting plate.

[0021] In one of the embodiments, a slider and a guide rail are further included, the guide rail is arranged on the side of the heat cover support close to the second mounting plate, and the guide rail is arranged in the second direction.

[0022] The slider is installed on the side of the second mounting plate close to the heat cover support, and the second mounting plate is in sliding connection with the guide rail through the slider.

[0023] The rotating member is movably connected with the first connecting part of the heat cover support through the rotating shaft, so that it can float in the first direction; the first inclined surface of the moving member is in contact with the rotating member, and part of the displacement in the first direction is converted into displacement in the second direction; the first elastic member provides elastic force in the first direction; the first elastic member is compressed to generate constant pressure, which is applied to each reaction tube through the roller, so as to absorb the size change caused by height tolerance, thermal expansion and the like, thereby maintaining constant pressure and solving the problem of uneven pressure caused by the tolerance of the reaction tube. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of the constant pressure heat cover device for the gene amplification instrument.

[0025] Figure 2 It is a front view schematic diagram of the constant pressure heat cover device for the gene amplification instrument.

[0026] Figure 3 It is a sectional view schematic diagram of the constant pressure heat cover device for the gene amplification instrument.

[0027] In the figure: 10, heat cover assembly; 11, mounting cavity;

[0028] 20, heat cover support; 21, first connecting part; 211, sliding groove; 22, second connecting part; 221, guide groove;

[0029] 30, rotating member; 31, roller; 32, rotating shaft;

[0030] 40, moving member; 41, first inclined surface; 42, guide rod; 43, wedge block; 44, guide block;

[0031] 51, first elastic member; 52, second elastic member;

[0032] 61, first mounting plate; 62, second mounting plate; 63, screw; 64, limit post; 65, slider; 66, guide rail; 67, POGOPIN contact pin. DETAILED DESCRIPTION

[0033] In order to make the above objectives, features and advantages of the present application more clear and comprehensible, the specific embodiments of the present application will be described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be practiced in a number of different ways beyond the specific embodiments described herein and by one of ordinary skill in the art without departing from the spirit and scope of the present application, and it is therefore intended that all such variations be considered as falling within the scope of the present application. Accordingly, the present application should not be limited by the following description and examples.

[0034] In the description of the present application, it should be understood that, if there are terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0035] In addition, if the terms "first", "second" appear, these terms are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implying the number of technical features indicated. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, if the term "multiple" appears, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise explicitly specified.

[0036] In the present application, unless otherwise explicitly specified and limited, if the terms "mounting", "connecting", "connecting", "fixing" and the like appear, these terms should be interpreted broadly. For example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0037] In the present application, unless otherwise explicitly specified and limited, if there is a description such as "on" or "under" or the like between a first feature and a second feature, it can mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature can be "above", "over" and "on" the second feature, which can mean that the first feature is directly above or obliquely above the second feature, or only means that the first feature is higher in horizontal height than the second feature. The first feature can be "below", "under" and "under" the second feature, which can mean that the first feature is directly below or obliquely below the second feature, or only means that the first feature is lower in horizontal height than the second feature.

[0038] It should be noted that if an element is referred to as "fixed to" or "provided on" another element, it can be directly on the other element or there can be a middle element. If an element is considered to be "connected" to another element, it can be directly connected to the other element or there can be a middle element. If present, the terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used in the present application are only for illustrative purposes and do not represent the only implementation.

[0039] Referring to Figure 1 , Figure 2 , Figure 1 A structural schematic diagram of a constant pressure thermal cover device for a gene amplification instrument in an embodiment of the present application is shown. Figure 2 A front view schematic diagram of a constant pressure thermal cover device for a gene amplification instrument in an embodiment of the present application is shown.

[0040] An embodiment of the present application provides a constant pressure thermal cover device for a gene amplification instrument, which comprises a thermal cover assembly 10, a thermal cover support 20, a rotating member 30 and a moving member 40, and realizes automatic and self-adaptive constant pressure compression of a reaction tube to be detected.

[0041] In the present embodiment, the thermal cover assembly 10 comprises at least one mounting cavity 11 for placing a reaction tube. The thermal cover support 20 comprises a first connecting part 21 and a second connecting part 22, and the first connecting part 21 is movably connected with the thermal cover assembly 10. The rotating member 30 is rotatably arranged above the mounting cavity 11 and connected between the two first connecting parts 21. The moving member 40 is arranged on one side of the rotating member 30 and movably connected with the second connecting part 22. The moving member 40 is provided with a first inclined surface 41 for abutting on the rotating member 30.

[0042] The first elastic member 51 is arranged between the thermal cover support 20 and the rotating member 30. When the thermal cover assembly 10 is pressed on the reaction tube, the reaction tube pushes the rotating member 30 to rotate and move in a first direction, and pushes the moving member 40 to move in a second direction.

[0043] The thermal cover assembly 10 is provided with a heating element inside for maintaining a temperature higher than the boiling point of the liquid in the reaction tube during operation to prevent condensation. The thermal cover assembly 10 is provided with at least one mounting cavity 11 for accommodating the cap portion of the PCR reaction tube. The thermal cover assembly 10 is connected to the thermal cover support 20 through a mounting plate, and can be opened or closed relative to the instrument.

[0044] The thermal cover support 20 is arranged on the thermal cover assembly 10. The thermal cover support 20 includes first and second connecting portions 21 and 22 on both sides. The first connecting portion 21 extends downward, and the thermal cover support 20 is movably connected to the thermal cover assembly 10 through the first connecting portion 21, so that the thermal cover assembly 10 has a certain upward floating stroke in the first direction, i.e. the vertical direction.

[0045] The rotating member 30 is a roller 31. The roller 31 is rotatably arranged above the mounting cavity 11 of the thermal cover assembly 10 through a rotating shaft 32. The two ends of the rotating shaft 32 are respectively connected between the two first connecting portions 21, so that the rotating member 30 can freely rotate around its own axis. The outer circumferential surface of the roller 31 serves as a rolling working surface in contact with the reaction tube below or the moving member 40 above.

[0046] The moving member 40 is a wedge block 43 and a guide rod 42. The moving member 40 is arranged on one side of the rotating member 30 and movably connected to the second connecting portion 22 of the thermal cover support 20. The moving member 40 is provided with a first inclined surface 41 on the side facing the rotating member 30. The first inclined surface 41 is used to abut on the outer circumferential surface of the roller 31 of the rotating member 30.

[0047] The first elastic member 51 is arranged between the thermal cover support 20 and the rotating member 30, and is used to provide a pre-tightening force in the vertical direction upward, so that the rotating member 30 has a tendency to float upward. The limiting column 64 is arranged between the rotating member 30 and the thermal cover support 20, and limits the rotating member 30 when it is floated upward in the vertical direction by the reaction tube. The pre-set height difference between the top of the limiting column 64 and the contact surface of the thermal cover support 20 determines the compression amount of the first elastic member 51 in the compressed state, thereby corresponding to the pre-set constant pressure value.

[0048] In the implementation process, when the hot cover is opened and the reaction tube is placed, the hot cover assembly 10 starts to be pressed down. When the first inclined surface 41 of the moving piece 40 contacts the rotating piece 30, the rotating piece 30 contacts the top of the reaction tube cover. Due to the height tolerance of different reaction tubes, the higher tube will lift the corresponding roller 31 higher. When the roller 31 is lifted, it will roll along the first inclined surface 41. The hot cover support 20 is blocked in the vertical direction by the limiting column 64, and the compression spring is compressed to a fixed compression amount. According to Hooke's law (F=kx), this fixed compression amount makes the compression spring generate a constant, vertically upward force F. This force is transmitted to the roller 31 through the hot cover support 20 and the rotating shaft 32, and is applied to the reaction tube cover.

[0049] Since the vertical position of the hot cover support 20 is fixed by the limiting column 64, the vertical position of the roller 31 is also fixed. The upward support force of the reaction tube cover on the roller 31 is balanced with the constant force F of the compression spring downward. If a certain reaction tube is slightly higher, the additional pressure will not increase by pushing the moving piece 40 to move horizontally to make room; if a certain reaction tube is slightly lower, the roller 31 will have a falling space on it, but the compression amount of the compression spring and the generated force remain unchanged due to the existence of the limiting column 64, ensuring that the pressure is constant.

[0050] In this state, the first inclined surface 41 of the moving piece 40 is in contact with the roller 31 and is locked in a constant pressure state. Small position changes caused by thermal expansion or mechanical relaxation will be absorbed by the horizontal movement of the moving piece 40, thereby maintaining the constant compression amount and output pressure of the compression spring in the vertical direction.

[0051] Through the above arrangement, automatic adaptation to reaction tubes of different heights is achieved in one cover closing operation, and the pressing force on each reaction tube is automatically adjusted and locked at a preset constant value, solving the problem of uneven pressure of the fixed pressure hot cover, improving reliability; and the overall structure is simple and easy to operate, thereby reducing the use cost and improving the user experience, ensuring the consistency of the sealing pressure of the reaction system in the PCR experiment process, and providing protection for the accuracy of the experimental results.

[0052] In combination with Figure 2 As shown in the figure, Figure 2 is a front view of a constant pressure hot cover device for a gene amplification instrument provided in an embodiment of the present application. In one embodiment, the first connecting part 21 is provided with a sliding groove 211 near one end of the hot cover assembly 10; the rotating piece 30 is a roller 31, and the roller 31 is connected to the two sliding grooves 211 through a rotating shaft 32. The sliding groove 211 is arranged in extension along the first direction, and the two ends of the rotating shaft 32 are arranged in the sliding grooves 211 and move along the first direction.

[0053] Specifically, the first connecting part 21 is provided with a sliding groove 211 near one end of the hot cover assembly 10. The sliding groove 211 is arranged on the downwardly extending part of the first connecting part 21, and is an elongated opening or groove extending in the first direction. The sliding groove 211 is arranged to extend in the first direction, and the first direction is the vertical direction, i.e. the direction of the movement of the hot cover assembly 10 when it is pressed down and lifted up.

[0054] The rotating member 30 is a roller 31, which is installed through a rotating shaft 32. The rotating shaft 32 penetrates the center hole of the roller 31, and its two ends are arranged in the corresponding sliding grooves 211 of the two first connecting parts 21, so that the roller 31 can move in the first direction in the sliding grooves 211.

[0055] The sliding grooves 211 provide a track for the upward and downward movement of the roller 31 and the rotating shaft 32, and ensure the stability of the movement of the roller 31 in the vertical direction, avoiding possible jamming or deviation. The length of the sliding grooves 211 limits the maximum floating stroke of the roller 31 in the vertical direction. When the reaction tube lifts the roller 31 upward, the rotating shaft 32 slides upward along the sliding grooves 211; when the first elastic member 51 pushes the roller 31 to reset downward, the rotating shaft 32 can slide downward along the sliding grooves 211, ensuring that it can adapt to the height tolerance of various reaction tubes.

[0056] In one embodiment, the second connecting part 22 is provided with a guide groove 221 near one end of the hot cover assembly 10, and the axis direction of the guide groove 221 has an included angle with the first direction; the moving member 40 is a guide rod 42, one end of the guide rod 42 is provided with a wedge block 43, and the side of the wedge block 43 close to the roller 31 is provided with a first inclined surface 41; the other end of the guide rod 42 is movably connected to the guide groove 221.

[0057] Specifically, the second connecting part 22 is provided with a guide groove 221 near one end of the hot cover assembly 10. The axis direction of the guide groove 221 has a non-zero included angle with the first direction, i.e. the vertical direction. The included angle makes the guide groove 221 used for guiding and restricting the movement track of the moving member 40 when the moving member 40 moves horizontally.

[0058] The moving member 40 includes the guide rod 42 and the wedge block 43, the guide rod 42 is arranged in the second direction, and the wedge block 43 is arranged at one end of the guide rod 42 close to the rotating member 30. The side of the wedge block 43 close to the roller 31 is provided with the first inclined surface 41, which is used to contact and move relative to the outer circumferential surface of the roller 31.

[0059] The other end of the guide rod 42 is movably connected to the guide groove 221, so that when the guide rod 42 and the wedge block 43 move in the second direction away from the side of the rotating member 30, the guide rod 42 can move along the axis direction of the guide groove 221.

[0060] In one embodiment, the second connecting part 22 is provided with a guide groove 221 on each side, and the guide groove 221 is a strip-shaped groove; the moving part 40 further comprises a guide block 44, which is installed on the guide rod 42 close to the second connecting part 22 and is movably connected to the guide groove 221 by a bolt.

[0061] Specifically, the second connecting part 22 is provided with a guide groove 221 on each side. The symmetrical bilateral guide can effectively prevent the moving part 40 from being twisted or stuck during movement, and ensure the linearity and stability of the movement track.

[0062] The guide groove 221 can be a strip-shaped groove. The moving part 40 further comprises a guide block 44. The guide block 44 is fixedly installed on the guide rod 42 close to the second connecting part 22. The guide block 44 is provided with a through hole, which is movably connected to the guide groove 221 by a bolt, a pin shaft or the like. For example, the shank of the bolt passes through the guide block 44, and the head or the nut has a size greater than the width of the guide groove 221, so that the moving part 40 is hung in the guide groove 221, and can freely slide along the length direction of the groove, while it cannot be taken out.

[0063] The guide groove 221 provides a movement path for the moving part 40, and ensures that when the roller 31 rolls along the first inclined surface 41 and pushes the wedge block 43, the wedge block 43 translates in the second direction, i.e. the horizontal direction, to ensure stable output of the constant pressure and improve the stability of the movement process. By restricting the movement degree of freedom of the moving part 40 through the guide groove 221, the relative position and angle of the contact area between the first inclined surface 41 and the roller 31 are kept stable, avoiding the problem of unstable pressure transmission caused by loose connection or deflection, so that the constant pressure control is more reliable. The detachable connection of the guide block 44 and the bolt facilitates the assembly, disassembly and maintenance of the device.

[0064] In combination with Figure 3 the drawings, Figure 3 is a sectional view of the constant pressure hot cover device for the gene amplification instrument provided in an embodiment of the present application. In one embodiment, it further comprises a first mounting plate 61 and a second mounting plate 62 movably connected, the first mounting plate 61 is arranged on the moving part 40, the second mounting plate 62 is fixedly arranged between the hot cover support 20 and the first mounting plate 61, and the first mounting plate 61 moves in the first direction.

[0065] Specifically, the first mounting plate 61 is a movable plate arranged on the moving part 40. The first mounting plate 61 is fixed on the wedge block 43 of the moving part 40, or the first mounting plate 61 is integrally formed with the wedge block 43 of the moving part 40. The first mounting plate 61 can move upward in the first direction, i.e. the vertical direction.

[0066] The second mounting plate 62 is a fixed plate or a relatively fixed plate, which is fixedly arranged between the thermal cover support 20 and the first mounting plate 61. The second mounting plate 62 is fixedly connected to one side of the thermal cover support 20 close to the first mounting plate 61. The first mounting plate 61 and the second mounting plate 62 are movably connected, so that the first mounting plate 61 is displaced in the vertical direction relative to the second mounting plate 62.

[0067] The first mounting plate 61 is provided with a POGOPIN contact pin 67, i.e., a spring probe, which forms a contact type electrical connection with the wedge block 43 of the moving part 40, and is used to trigger an indicator lamp when turned on, to indicate to the user that the reaction tube is in a state of applying a constant pressure.

[0068] In one embodiment, the first mounting plate 61 and the second mounting plate 62 are fixed by a plurality of screws 63; and further comprising a first elastic member 51, one end of the first elastic member 51 being connected to the second connecting portion, and the other end of the first elastic member 51 being connected to the screw 63.

[0069] Specifically, the first mounting plate 61 and the second mounting plate 62 are movably connected and relatively fixed by a plurality of screws 63. The screw rod part of the screw 63 passes through the through hole on the first mounting plate 61, and is screwed into the threaded hole on the second mounting plate 62, or passes through both and is locked by a nut.

[0070] The first mounting plate 61 and the second mounting plate 62 are connected by the screw 63. Tightening the screw 63 compresses the first elastic member 51, thereby increasing the preset pressure applied to the reaction tube; conversely, it reduces the pressure, so that the constant pressure value can be calibrated during assembly or maintenance, without the need to replace the spring to adapt to different types of reaction tubes, enhancing adaptability and maintainability.

[0071] The first elastic member 51 is a pressure spring or a wave spring, one end of which is connected to or abuts against the lower side of the second mounting plate 62, and the other end of which is connected to or abuts against the lower side of the nut of the screw 63, or a bearing surface related to the first mounting plate 61. The first elastic member 51 is pre-compressed between the second mounting plate 62 and the screw 63, providing an adjustable vertical pre-tightening force. By tightening or loosening the screw 63, the size of this pre-tightening force can be fine-tuned, thereby calibrating the initial state of the entire constant pressure system.

[0072] In one embodiment, further comprising a second elastic member 52, one end of the second elastic member 52 being connected to the moving part 40, and the other end of the second elastic member 52 being connected to the second connecting portion 22; the elastic force of the first elastic member 51 is greater than the elastic force of the second elastic member 52.

[0073] Specifically, one end of the second elastic member 52 is connected to the wedge block 43 of the moving member 40, and the other end is connected to the second connecting portion 22, and the axis direction is consistent with the second direction, for providing horizontal restoring force for the moving member 40. The elastic force or stiffness of the first elastic member 51 is greater than that of the second elastic member 52, which ensures that in the closing stage, the force of the roller 31 contacting and pushing the reaction tube will preferentially push the moving member 40 to compress the second elastic member 52 with smaller elasticity, rather than compressing the first elastic member 51 with larger elasticity, thereby providing space for reaction tubes of different heights, and realizing self-adaptation.

[0074] In one embodiment, a plurality of limiting columns 64 are further included, one end of the limiting column 64 is installed on the first mounting plate 61, and a gap exists between the other end of the limiting column 64 and the second mounting plate 62.

[0075] Specifically, the plurality of limiting columns 64 are vertically arranged, one end of which is fixedly installed on the first mounting plate 61, and the other end extends upward, and a predetermined gap exists between the bottom surface of the second mounting plate 62.

[0076] The gap of the limiting column 64 determines the maximum stroke of the first mounting plate 61, the moving member 40 and the roller 31 in the vertical direction. When the hot cover is pressed to the final position, the top end of the limiting column 64 on the first mounting plate 61 contacts the bottom of the second mounting plate 62, forming a limit. At this time, the first elastic member 51 is compressed to a fixed compression amount determined by the gap. According to Hooke's law, this compression amount generates a constant vertical force F, which is finally applied to the reaction tube cover through the moving member 40 and the roller 31.

[0077] In the constant pressure locking state, the size changes caused by thermal expansion, material creep or slight vibration are first converted into a slight relative displacement trend between the first mounting plate 61 and the second mounting plate 62, and are offset by the restoring force of the first elastic member 51, or are absorbed by the second elastic member 52 through the slight horizontal displacement of the moving member 40, which ensures that the pressure applied to the reaction tube can be continuously and stably maintained at a preset value in a dynamic working environment, thereby ensuring the constant pressure.

[0078] In one embodiment, a sliding block 65 and a guide rail 66 are further included, the guide rail 66 is arranged on the side of the hot cover support 20 close to the second mounting plate 62, and the guide rail 66 is arranged along the second direction; the sliding block 65 is installed on the side of the second mounting plate 62 close to the hot cover support 20, and the second mounting plate 62 is slidably connected with the guide rail 66 through the sliding block 65.

[0079] Specifically, the guide rail 66 is arranged on the side of the hot cover support 20 close to the second mounting plate 62. The length direction of the guide rail 66 is arranged along the second direction, that is, parallel to the movement direction of the moving member 40.

[0080] The slider 65 is fixedly installed on the second mounting plate 62 near one side of the thermal cover support 20. By installing the slider 65 on the second mounting plate 62, the second mounting plate 62 is slidably connected with the guide rail 66 through the slider 65, and a track is provided for the horizontal movement of the second mounting plate 62. When the roller 31 pushes the wedge block 43, the moving piece 40 and the second mounting plate 62 can move horizontally, and the vertical displacement is converted into horizontal displacement.

[0081] The slider 65 and the guide rail 66 pair can effectively eliminate the freedom and clearance of the moving part in the non-movement direction, and ensure that the second mounting plate 62 does not float up and down or swing left and right when sliding horizontally. The gap height between the limiting column 64 fixed to the second mounting plate 62 and the second mounting plate 62 is kept constant throughout the process, and the dynamic stability is higher, which guarantees the continuous constancy of the pressure environment in the PCR reaction process.

[0082] The technical features of the above embodiments can be combined arbitrarily. In order to make the description simple, all possible combinations of the technical features in the above embodiments are not described, but as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the present application.

[0083] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the patent application scope. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A constant pressure heating cap device for a gene amplification instrument, characterized in that, include: A heated cap assembly includes at least one mounting cavity for placing a reaction tube; The heat cover bracket includes a first connecting part and a second connecting part, wherein the first connecting part is movably connected to the heat cover assembly; A rotating component is rotatably disposed above the mounting cavity and connected between the two first connecting portions; A movable component is disposed on one side of the rotating component and is movably connected to the second connecting part; the movable component is provided with a first inclined surface, which is used to abut against the rotating component; A first elastic element is disposed between the heat cover bracket and the rotating element; When the hot cap assembly is pressed onto the reaction tube, the reaction tube pushes the rotating member to rotate and move along the first direction, and pushes the moving member to move along the second direction.

2. The constant pressure heating cap device for a gene amplification instrument according to claim 1, characterized in that, The first connecting part has a groove at one end near the hot cover assembly; the rotating part is a roller, and the roller is connected between the two grooves through a rotating shaft.

3. The constant pressure heating cap device for a gene amplification instrument according to claim 2, characterized in that, The slide groove extends along a first direction, and the two ends of the rotating shaft are disposed within the slide groove and move along the first direction.

4. The constant pressure heating cap device for a gene amplification instrument according to claim 2, characterized in that, The second connecting part has a guide groove at one end near the hot cover assembly, and the axial direction of the guide groove has an angle with the first direction; the moving part is a guide rod, one end of the guide rod has a wedge, and the side of the wedge near the roller has a first inclined surface; the other end of the guide rod is movably connected to the guide groove.

5. The constant pressure heating cap device for a gene amplification instrument according to claim 4, characterized in that, The second connecting part has guide grooves on both sides, and the guide grooves are strip-shaped grooves; the moving part also includes a guide block, which is installed on the end of the guide rod near the second connecting part and is movably connected to the guide groove by bolts.

6. The constant pressure heating cap device for a gene amplification instrument according to claim 2, characterized in that, It also includes a first mounting plate and a second mounting plate that are movably connected vertically. The first mounting plate is disposed on the movable part, and the second mounting plate is fixedly disposed between the heat cover bracket and the first mounting plate. The first mounting plate moves along a first direction.

7. The constant pressure heating cap device for a gene amplification instrument according to claim 6, characterized in that, The first mounting plate and the second mounting plate are fixed together by a plurality of screws; it also includes a first elastic member, one end of which is connected to the second connecting part, and the other end of which is connected to the screws.

8. The constant pressure heating cap device for a gene amplification instrument according to claim 7, characterized in that, It also includes a second elastic element, one end of which is connected to the moving element, and the other end of which is connected to the second connecting portion; the elastic force of the first elastic element is greater than the elastic force of the second elastic element.

9. The constant pressure heating cap device for a gene amplification instrument according to claim 8, characterized in that, It also includes multiple limiting posts, one end of which is mounted on the first mounting plate, and the other end of which has a gap with the second mounting plate.

10. The constant pressure heating cap device for a gene amplification instrument according to claim 2, characterized in that, It also includes a slider and a guide rail, the guide rail being disposed on the side of the heat cover bracket near the second mounting plate, the guide rail being disposed along the second direction; The slider is mounted on the side of the second mounting plate near the heat cover bracket, and the second mounting plate is slidably connected to the guide rail via the slider.

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