Cross contamination prevention centrifugal device for gene detection sample nucleic acid extraction

Abstract

The invention discloses an anti-cross contamination centrifugal device for gene detection sample nucleic acid extraction, and relates to the technical field of gene detection sample nucleic acid extraction, the anti-cross contamination centrifugal device comprises a device base and an isolation member, the top of the device base is inserted and butted with a shield member, and the isolation member is vertically connected to one end of the top of a driving member. According to the anti-cross-contamination centrifugal device for nucleic acid extraction of the gene detection sample, cross contamination is effectively prevented during nucleic acid extraction of the gene detection sample through the combined design of all the structures, the protective cover component is tightly inserted into the top of the base, a relatively closed space is formed, external impurities are blocked, and sample contamination is avoided. The driving component is embedded in the middle of the top of the base and provides stable and strong power for centrifugation, and it is ensured that operation is efficient and accurate. The isolation component is vertically connected to one end of the top of the driving component to isolate different sample areas, so that mutual interference and cross contamination are prevented, and nucleic acid extraction accuracy and reliability are improved.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid extraction technology for gene detection samples, specifically to a centrifuge device for preventing cross-contamination in the extraction of nucleic acid from gene detection samples. Background Technology

[0002] Nucleic acid extraction for gene testing is the process of isolating and purifying DNA or RNA from biological samples (such as blood, saliva, and tissues), and it is a crucial step in gene testing. Its core involves lysing cells to release nucleic acids and removing impurities such as proteins and salts to ensure the integrity and purity of the nucleic acids. Commonly used methods include phenol-chloroform extraction, magnetic bead extraction, and automated extraction, each with its own advantages and disadvantages in terms of cost, efficiency, and purity. The extracted nucleic acids are used for subsequent analyses such as PCR and sequencing, providing a foundation for disease diagnosis and genetic research.

[0003] While conventional centrifugation devices can achieve sample separation during nucleic acid extraction for gene testing, they have several shortcomings. Firstly, conventional centrifugation devices lack effective cross-contamination prevention designs. During centrifugation, cross-contamination can easily occur between different samples due to aerosol diffusion, centrifuge tube breakage, and other factors. This leads to impure extracted nucleic acid samples, affecting the accuracy of subsequent gene testing and causing deviations in test results, thus failing to provide reliable evidence for disease diagnosis and genetic research. Summary of the Invention

[0004] The purpose of this invention is to provide a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples, comprising a device base and an isolation component. A protective cover component is inserted and connected to the top of the device base, and a driving component is embedded in the middle of the top of the device base. The isolation component is vertically connected to one end of the top of the driving component. The isolation component includes a lower barrier sleeve, a support pile, an upper barrier sleeve, a fixing bolt, a sealing ring, and a docking pin. The bottom of the lower barrier sleeve is vertically provided with a support pile, and the top of the lower barrier sleeve is vertically connected and connected to the upper barrier sleeve. Fixing bolts are vertically threaded at both ends of the connection between the lower barrier sleeve and the upper barrier sleeve. A sealing ring is fixed in the middle of the surface of the lower barrier sleeve near the upper barrier sleeve, and docking pins are vertically and integrally connected at the four diagonal points of the upper barrier sleeve near the upper barrier sleeve.

[0006] Furthermore, the device base includes a base body, a control panel, mounting holes, a stabilizing groove, and fixed feet. The control panel is inlaid on the front surface of the base body, and mounting holes are evenly and vertically opened at the four diagonal corners of the top surface of the base body. A stabilizing groove is vertically opened on the middle top surface of the base body, and fixed feet are symmetrically fixed at the lower ends of the left and right sides of the base body.

[0007] Furthermore, both ends of the fixed support leg are vertically provided with holes for bolt installation, and the fixed support leg and the base body are integrally formed.

[0008] Furthermore, the protective cover component includes a main cover body, anti-collision plates, and mounting pins. Anti-collision plates are vertically fixed on all four inner surfaces of the main cover body, and mounting pins are vertically connected at the four opposite corners of the bottom of the main cover body.

[0009] Furthermore, the assembly pin and the assembly hole are connected by a snap-fit ​​insertion structure, and the assembly pin and the main cover are integrated into one structure. Moreover, the top of the main cover is transparent.

[0010] Furthermore, the driving component includes a brushless direct drive motor, a fixed base, a rotating frame, and a fixed stake. The bottom of the brushless direct drive motor is integrally provided with a fixed base, and the top power output end of the brushless direct drive motor is fixedly connected to the rotating frame. The rotating frame is integrally provided with fixed stakes around it.

[0011] Furthermore, the brushless direct drive motor is vertically installed in the stabilizing groove via a fixed base, and the fixed piles are distributed in a circular array structure with the rotating frame as the center, with six groups arranged.

[0012] Furthermore, the fixed pile and the support pile are connected by threads, and the support pile and the lower barrier sleeve are integrally set. The upper barrier sleeve has four diagonal holes on one side surface near the upper barrier sleeve for inserting the piles.

[0013] This invention provides a cross-contamination-preventing centrifugation device for nucleic acid extraction from gene detection samples, which has the following beneficial effects: 1. This invention, through the structural design of the isolation components, specifically the tight connection between the lower and upper isolation sleeves via fixing bolts and the sealing ring in between, effectively prevents leakage of the sample container during centrifugation, ensuring sample integrity and purity and avoiding the risk of cross-contamination. Furthermore, the docking pins cooperate with the insertion holes on the upper isolation sleeve, allowing for a stable and quick docking and assembly of the two sleeves, facilitating disassembly and cleaning, thus improving the ease of use and hygiene of the device. Additionally, the lower isolation sleeve utilizes a bottom support post for threaded connection with a fixing post on the drive component, achieving a stable connection between the isolation component and the drive component. This connection method not only enhances the overall stability of the device but also allows the isolation component to rotate with the drive component, enabling centrifugation of the sample. It also facilitates easy assembly and disassembly of the components, allowing for thorough cleaning and maintenance of the device's interior, further ensuring sample purity and preventing cross-contamination.

[0014] 2. This invention, through the structural arrangement of the device base and protective cover components, provides a solid foundation for the entire centrifugal device through its stable base body and fixed supports, ensuring the stability and safety of the device during high-speed rotation. Furthermore, the protective cover component, with its main body and anti-collision plates, effectively protects the internal drive and isolation components from external collisions. Its transparent top design allows operators to directly observe the internal operating status of the device, facilitating timely monitoring and adjustment. In addition, the protective cover component achieves a stable connection with the device base through a locking and engaging structure where mounting pins interlock with mounting holes on the device base. This design not only enhances the overall structural strength of the device but also simplifies and facilitates the installation and disassembly of the protective cover component, making daily cleaning and maintenance easier and further improving the device's usability and hygiene.

[0015] 3. This invention, through the structural arrangement of the driving component and the isolation component, utilizes a brushless direct-drive motor as the power source for the driving component. This motor offers advantages such as high efficiency, stability, and low noise, providing continuous and stable rotational power to the isolation component. This ensures uniform force on the sample during centrifugation, improving the quality and efficiency of nucleic acid extraction. Furthermore, the brushless direct-drive motor is vertically mounted in the stabilizing tank via a fixed base. This mounting method effectively buffers and absorbs vibrations generated during motor operation, further enhancing the stability of the device. Simultaneously, the fixed posts around the rotating frame are arranged in a ring array and threadedly connected to the support posts on the isolation component. This design not only allows the isolation component to be securely mounted on the driving component but also enables it to rotate together with the driving component, facilitating sample centrifugation. Moreover, this structural arrangement facilitates the disassembly and replacement of the isolation component to adapt to the processing needs of samples of different specifications and quantities, improving the flexibility and practicality of the device. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main body of a cross-contamination prevention centrifugation device for nucleic acid extraction from gene detection samples according to the present invention; Figure 2 This is a schematic diagram of the main body structure of a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to the present invention; Figure 3 This is a three-dimensional structural diagram of the base of a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to the present invention; Figure 4 This is a three-dimensional structural diagram of the protective cover component of a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to the present invention; Figure 5 This is a three-dimensional structural diagram of the driving component of a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to the present invention; Figure 6 This is a three-dimensional structural diagram of the isolation component of a centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to the present invention.

[0017] In the diagram: 1. Device base; 101. Base body; 102. Control panel; 103. Assembly socket; 104. Stabilizing groove; 105. Fixed support leg; 2. Protective cover component; 201. Main cover; 202. Anti-collision plate; 203. Assembly plug; 3. Drive component; 301. Brushless direct drive motor; 302. Fixed seat; 303. Rotating frame; 304. Fixed pile; 4. Isolation component; 401. Lower isolation sleeve; 402. Support pile; 403. Upper isolation sleeve; 404. Fixing bolt; 405. Sealing ring; 406. Connecting plug. Detailed Implementation

[0018] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0019] like Figures 1 to 6 As shown, a cross-contamination prevention centrifuge device for nucleic acid extraction from gene detection samples includes a device base 1 and an isolation component 4. A protective cover component 2 is inserted and connected to the top of the device base 1, and a driving component 3 is embedded in the middle of the top of the device base 1. The isolation component 4 is vertically connected to one end of the top of the driving component 3. The isolation component 4 includes a lower barrier sleeve 401, a support pile 402, an upper barrier sleeve 403, a fixing bolt 404, a sealing ring 405, and a docking pin 406. The support pile 402 is vertically arranged at the bottom of the lower barrier sleeve 401, and the upper barrier sleeve 403 is vertically connected and connected to the top of the lower barrier sleeve 401. The lower barrier sleeve 401 and the upper barrier sleeve 403 are connected and connected. Both ends of the 403 connection are vertically threaded with fixing bolts 404, and a sealing ring 405 is fixed in the middle of the surface of the lower barrier sleeve 401 near the upper barrier sleeve 403. Simultaneously, four interlocking pins 406 are vertically and integrally connected at the four diagonal points of the upper barrier sleeve 403 near the upper barrier sleeve 403. The drive component 3 includes a brushless direct drive motor 301, a fixed base 302, a rotating frame 303, and fixing pins 304. The bottom of the brushless direct drive motor 301 is integrally provided with the fixed base 302, and the top power output end of the brushless direct drive motor 301 is fixedly connected to the rotating frame 303. Fixing pins 304 are integrally provided around the rotating frame 303. The motor 301 is vertically mounted in the stabilizing groove 104 via the fixing base 302. The fixing piles 304 are arranged in a circular array around the rotating frame 303, with six groups arranged in this array. The fixing piles 304 and the supporting piles 402 are connected by threads, and the supporting piles 402 and the lower barrier sleeve 401 are integrally formed. The upper barrier sleeve 403 has four diagonal openings on its side surface near the upper barrier sleeve 403 for inserting the connecting piles 406. When the brushless direct-drive motor 301 starts, its power is transmitted to the fixing piles 304 through the rotating frame 303. Since the fixing piles 304 and the supporting piles 402 are connected by threads, and the supporting piles 402 and the lower barrier sleeve... Since tube 401 is integrally set, the entire isolation component 4 will rotate synchronously with the rotation of the driving component 3. During the rotation, the lower isolation sleeve 401 and the upper isolation sleeve 403 are tightly connected by the fixing bolt 404, and the sealing ring 405 set in the middle effectively prevents leakage of the sample container during centrifugation, ensuring the integrity and purity of the sample. At the same time, the docking post 406 cooperates with the insertion hole structure opened on the upper isolation sleeve 403, so that the lower isolation sleeve 401 and the upper isolation sleeve 403 can be firmly and quickly docked and installed. This design not only improves the structural stability of the device, but also facilitates disassembly and cleaning, improving the ease of use and hygiene of the device.

[0020] The device base 1 includes a base body 101, a control panel 102, mounting holes 103, a stabilizing groove 104, and fixed supports 105. The control panel 102 is embedded in the front surface of the base body 101. Mounting holes 103 are evenly and vertically formed at the four diagonal corners of the top surface of the base body 101. A stabilizing groove 104 is vertically formed on the middle top surface of the base body 101. Fixed supports 105 are symmetrically fixed to the lower ends of both sides of the base body 101. Both ends of the fixed supports 105 have vertical holes for bolt installation. The fixed supports 105 are connected to the base body. The protective cover component 2 comprises a main cover 201, anti-collision plates 202, and mounting pins 203. Anti-collision plates 202 are vertically fixed to the four inner surfaces of the main cover 201, and mounting pins 203 are vertically connected to the four opposite corners of the bottom of the main cover 201. The mounting pins 203 and mounting holes 103 are connected by a snap-fit ​​insertion structure, and the mounting pins 203 and the main cover 201 are integrally formed. Furthermore, the top of the main cover 201 is transparent, allowing operators to monitor the equipment via the control panel 102 on the front side of the base body 101 during operation. The parameters of the cardiac device are precisely set and controlled, such as rotation speed and running time, to meet the needs of nucleic acid extraction from different gene detection samples. The mounting holes 103 at the four opposite corners of the top of the base body 101 engage and connect with the mounting pins 203 at the four opposite corners of the bottom of the protective cover component 2. This stable connection method ensures that the protective cover component 2 will not loosen or fall off due to vibration during device operation. At the same time, the stabilizing groove 104 in the middle of the top of the base body 101 provides a stable mounting position for the brushless direct drive motor 301 of the drive component 3, which is combined with the bolt mounting holes at both ends of the fixed support 105. The entire device is firmly fixed to the workbench, further enhancing its stability during high-speed rotation. The anti-collision plates 202, which are vertically fixed on the four sides inside the main body 201 of the protective cover component 2, can effectively buffer and absorb the impact force that may be caused to the internal driving component 3 and isolation component 4 by the outside world, protecting the internal precision components from damage. The transparent structure design at the top of the main body 201 allows the operator to clearly observe the rotation status of the isolation component 4 and the placement of the sample container inside the device without opening the cover, making it easy to detect abnormalities in time and make corresponding adjustments, ensuring the smooth progress of the nucleic acid extraction process of gene detection samples.

[0021] In summary, as Figures 1 to 6 As shown, the cross-contamination prevention centrifuge device for nucleic acid extraction of gene detection samples is first used to securely fix the device to the workbench by means of bolt holes at both ends of the fixed support 105, ensuring that the device will not be displaced due to vibration during operation. Next, based on the quantity and specifications of the gene testing samples, select an appropriate number and specifications of lower barrier sleeves 401 and upper barrier sleeves 403, connect them tightly together with fixing bolts 404, and fix a sealing ring 405 in the middle of the joint to prevent leakage of the sample container placed inside during centrifugation. Then, the connected isolation component 4 is threadedly connected to the fixed pile 304 on the drive component 3 via the support pile 402, ensuring that the isolation component 4 can be stably installed on the drive component 3 and can rotate together with the drive component 3. Then, the protective cover component 2 is engaged and inserted into the four opposite corner mounting pins 203 at the bottom of its bottom and the four opposite corner mounting holes 103 at the top of the device base 1, so that the protective cover component 2 is firmly installed on the device base 1. At the same time, the anti-collision plates 202 vertically fixed on the four sides inside the protective cover component 2 can effectively protect the internal drive component 3 and the isolation component 4 from damage by external collisions. The transparent structure design at the top of the main cover 201 makes it convenient for operators to observe the internal operating status of the device. Finally, the operator precisely sets and controls various parameters of the centrifuge device, such as rotation speed and running time, through the control panel 102 on the front side of the base body 101. After setting, the brushless direct drive motor 301 is started, and its power is transmitted to the fixed pile 304 through the rotating frame 303, thereby driving the entire isolation component 4 to rotate and realize the centrifugation of the sample. During the centrifugation process, the sample condition can be observed at any time through the transparent top. After the centrifugation is completed, the device is disassembled in reverse order, and each component is cleaned and disinfected for the next use.

[0022] The embodiments of the present invention are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the invention to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical application of the invention, and to enable those skilled in the art to understand the invention and to design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples, comprising a device base (1) and an isolation component (4), characterized in that: A protective cover component (2) is inserted and connected to the top of the device base (1), and a driving component (3) is embedded in the middle of the top of the device base (1). The isolation component (4) is vertically connected to one end of the top of the driving component (3). The isolation component (4) includes a lower barrier sleeve (401), a support pile (402), an upper barrier sleeve (403), a fixing bolt (404), a sealing ring (405), and a docking pile (406). A support pile (406) is vertically arranged at the bottom of the lower barrier sleeve (401). 2), and the top of the lower barrier sleeve (401) is vertically connected to the upper barrier sleeve (403), and the left and right ends of the connection between the lower barrier sleeve (401) and the upper barrier sleeve (403) are vertically threaded with fixing bolts (404), and a sealing ring (405) is fixed in the middle of the side surface of the lower barrier sleeve (401) near the upper barrier sleeve (403), while the four diagonal corners of the side of the upper barrier sleeve (403) near the upper barrier sleeve (403) are vertically and integrally connected with docking pins (406).

2. The cross-contamination-preventing centrifuge device for nucleic acid extraction from gene detection samples according to claim 1, characterized in that, The device base (1) includes a base body (101), a control panel (102), an assembly socket (103), a stabilizing groove (104), and fixed feet (105). The control panel (102) is inlaid on the front surface of the base body (101), and the assembly socket (103) is evenly and vertically opened at the four diagonal corners of the top surface of the base body (101). The stabilizing groove (104) is vertically opened on the middle surface of the top of the base body (101), and fixed feet (105) are symmetrically fixed at the lower ends of the left and right sides of the base body (101).

3. The cross-contamination-proof centrifuge device for nucleic acid extraction from gene detection samples according to claim 2, characterized in that, Both ends of the fixed support (105) are vertically provided with holes for bolt installation, and the fixed support (105) and the base body (101) are integrally structured.

4. The cross-contamination-proof centrifuge device for nucleic acid extraction from gene detection samples according to claim 2, characterized in that, The protective cover component (2) includes a main cover (201), a crash plate (202) and a mounting pin (203). The crash plate (202) is vertically fixed on all four sides of the interior of the main cover (201), and the mounting pin (203) is vertically connected at the four opposite corners of the bottom of the main cover (201).

5. The centrifuge device for preventing cross-contamination of nucleic acid extraction from gene detection samples according to claim 4, characterized in that, The assembly pin (203) and the assembly hole (103) are connected by a snap-fit ​​insertion structure, and the assembly pin (203) and the main cover (201) are integrated into one structure. The top of the main cover (201) is transparent.

6. The cross-contamination-preventing centrifuge device for nucleic acid extraction from gene detection samples according to claim 2, characterized in that, The drive component (3) includes a brushless direct drive motor (301), a fixed base (302), a rotating frame (303), and a fixed post (304). The bottom of the brushless direct drive motor (301) is integrally provided with a fixed base (302), and the top power output end of the brushless direct drive motor (301) is fixedly connected to the rotating frame (303). The rotating frame (303) is integrally provided with a fixed post (304) around it.

7. The cross-contamination-proof centrifuge device for nucleic acid extraction from gene detection samples according to claim 6, characterized in that, The brushless direct drive motor (301) is vertically installed in the stabilizing groove (104) through the fixing base (302). The fixing piles (304) are distributed in a ring array structure with the rotating frame (303) as the center and there are six groups.

8. The cross-contamination-proof centrifuge device for nucleic acid extraction from gene detection samples according to claim 6, characterized in that, The fixed pile (304) and the support pile (402) are connected by threads, and the support pile (402) and the lower barrier sleeve (401) are integrally set.

9. The cross-contamination-proof centrifuge device for nucleic acid extraction from gene detection samples according to claim 8, characterized in that, The upper barrier sleeve (403) has four diagonal openings on one side surface near the upper barrier sleeve (403) for inserting the docking stake (406).

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