Nutrient solution for promoting growth of rice Yuzhenxiang and culture method

Abstract

The invention provides a nutrient solution for promoting growth of rice Yuzhenxiang and a culture method, and belongs to the technical field of rice planting. According to the nutrient solution provided for the rice variety 'Yuzhenxiang', a specific amino acid combination is used in a common rice nutrient solution to replace part of inorganic nitrogen, so that the plant height, biomass, nitrogen content, chlorophyll content and single-plant dry weight of 'Yuzhenxiang' can be effectively improved, and the adaptability of plants to growth environments in different regions can be improved; and large-scale and intensive planting management and large-area popularization and planting of high-quality rice varieties can be better promoted.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of rice planting, in particular to a nutrient solution for promoting the growth of rice "Yuzhenxiang" and a culture method. BACKGROUND

[0002] The indica rice "Yuzhenxiang" has "high quality and disease resistance" as the core target, and has food taste quality and rice blast resistance. It has become a benchmark variety for promoting the development of high-quality rice industry in southern China, and has significantly improved market competitiveness. However, the plant type of "Yuzhenxiang" is upright or semi-upright compact plant type, which makes the plant structure relatively single, the group competition pressure large, the nutrient distribution uneven, and the growth and development of the plant easily blocked. In addition, "Yuzhenxiang" may have poor adaptability to the growth environment of different provinces or regions, and its yield and resistance potential need to be further tapped.

[0003] In addition, the traditional transplanting method has been gradually replaced by factory seedling raising, and an appropriate planting and culture method for the high-quality rice variety "Yuzhenxiang" is explored, which is beneficial to the further large-scale, intensive and factory planting of the variety. SUMMARY

[0004] In order to solve the above problems, the present application aims to provide a nutrient solution for promoting the growth of rice "Yuzhenxiang", wherein the nitrogen element concentration in the nutrient solution is 1-3 mM, the nitrogen element is derived from inorganic nitrogen and organic nitrogen, and the organic nitrogen is selected from any one of the following amino acid combinations: Combination A: arginine, alanine, phenylalanine, threonine, proline; Combination B: glutamine, aspartic acid, tyrosine, cysteine, glycine; Combination C: asparagine, alanine, methionine, tryptophan, serine.

[0005] In an embodiment, the amino acid concentration in combination A is: 0.1-0.5 mM arginine, 0.09-0.4 mM alanine, 0.08-0.3 mM phenylalanine, 0.08-0.5 mM threonine, and 0.09-0.6 mM proline.

[0006] For example, it can be: 0.15 mM arginine, 0.1 mM alanine, 0.1 mM phenylalanine, 0.1 mM threonine, and 0.1 mM proline.

[0007] In an embodiment, the amino acid concentration in combination B is: 0.08-0.5 mM glutamine, 0.1-0.6 mM aspartic acid, 0.1-0.6 mM tyrosine, 0.05-0.5 mM cysteine, and 0.08-0.5 mM glycine.

[0008] For example, 0.2 mM glutamine, 0.2 mM aspartic acid, 0.2 mM tyrosine, 0.1 mM cysteine, 0.1 mM glycine.

[0009] In one embodiment, the amino acid concentration in the combination C is: 0.1-0.6 mM asparagine, 0.05-0.5 mM alanine, 0.05-0.5 mM methionine, 0.05-0.5 mM tryptophan, 0.1-0.6 mM serine.

[0010] For example, 0.15 mM asparagine, 0.1 mM alanine, 0.1 mM methionine, 0.15 mM tryptophan, 0.2 mM serine.

[0011] In one embodiment, the organic nitrogen is preferably the amino acid combination in combination A or combination C, and more preferably combination A.

[0012] In one embodiment, the molar ratio of inorganic nitrogen to organic nitrogen is (1-2):(2-1), preferably 1:1.

[0013] In one embodiment, the inorganic nitrogen is ammonium salt and / or nitrate. For example, the ammonium salt can be ammonium chloride, ammonium bicarbonate, ammonium sulfate, ammonium nitrate, etc.; the nitrate can be sodium nitrate, potassium nitrate, calcium nitrate, ammonium nitrate, etc.

[0014] Preferably, the inorganic nitrogen source is NH4Cl and NaNO3.

[0015] In one embodiment, the nutrient solution further comprises small molecule nutrients selected from any one or several of the following: γ-aminobutyric acid, salicylic acid, melatonin, vitamin C, Br.

[0016] In one embodiment, the addition concentration of small molecule nutrients can use the commonly used concentration in the field of plant nutrient solution or fertilizer, for example: 0.5 mM γ-aminobutyric acid, 15 μM salicylic acid, 1 μM melatonin, 1 mM vitamin C, and 0.1 μM Br. In a preferred embodiment, the small molecule nutrients are compounded with the amino acid combination of combination A or combination B, and more preferably with combination B.

[0017] In one embodiment, the nutrient solution further comprises at least one inorganic salt containing any one of the following elements: phosphorus, potassium, sodium, calcium, magnesium, manganese, molybdenum, boron, zinc, copper, iron.

[0018] In one embodiment, the inorganic salt comprises hydrochloride, sulfite, sulfate, nitrate, phosphate, phosphite, pyrophosphate, acetate, borate, methanesulfonate, succinate, fumarate, citrate, gluconate, malate, or amine.

[0019] In one embodiment, the nutrient solution further comprises at least one of NaH2PO4•2H2O, K2SO4, CaCl2, MgSO4•7H2O, MnCl2•4H2O, Na2MoO4•2H2O, H3BO4, ZnSO4•7H2O, CuSO4•5H2O, EDTA-Na2, FeSO4•7H2O.

[0020] Preferably, the nutrient solution further comprises NaH2PO4•2H2O 60~65 mg / L, K2SO4 110~120 mg / L, CaCl2 35~45 mg / L, MgSO4•7H2O 500~510 mg / L, MnCl2•4H2O 1.5~2 mg / L, Na2MoO4•2H2O 0.1~0.5 mg / L, H3BO4 1~2 mg / L, ZnSO4•7H2O 0.01~0.06 mg / L, CuSO4•5H2O 0.01~0.08 mg / L, EDTA-Na2 12~20 mg / L, FeSO4•7H2O 10~15 mg / L. For example, NaH2PO4•2H2O 63.000 mg / L, K2SO4 111.625 mg / L, CaCl2 39.55 mg / L, MgSO4•7H2O 506.250 mg / L, MnCl2•4H2O 1.761 mg / L, Na2MoO4•2H2O 0.127 mg / L, H3BO4 1.168 mg / L, ZnSO4•7H2O 0.044 mg / L, CuSO4•5H2O 0.039 mg / L, EDTA-Na2 15.000 mg / L, FeSO4•7H2O 11.140 mg / L.

[0021] In one embodiment, the nutrient solution for promoting the growth of rice "Yu Zhen Xiang" provided by the present application contains the aforementioned NaH2PO4•2H2O and other substances, and further contains nitrogen elements, which are derived from inorganic nitrogen and organic nitrogen, and the organic nitrogen is a combination of amino acids of arginine, alanine, phenylalanine, threonine, and proline.

[0022] In an embodiment, the nutrient solution for promoting growth and development of rice "Yu Zhen Xiang" or for improving adaptability of rice "Yu Zhen Xiang" provided by the present application contains nitrogen elements and small molecule nutrients in addition to the aforementioned NaH2PO4·2H2O and the like, wherein the nitrogen elements are derived from inorganic nitrogen and organic nitrogen, and the organic nitrogen is a combination of glutamine, aspartic acid, tyrosine, cysteine and glycine.

[0023] The "improving adaptability of rice "Yu Zhen Xiang" can be understood as improving the growth and development of rice "Yu Zhen Xiang" under acid rain, saline-alkali, drought, high temperature, low temperature, disease, strong light or a combination of the above conditions.

[0024] It can be understood that the solvent used for preparing each component in the nutrient solution provided by the present application is water.

[0025] On the other hand, the present application also provides the use of the nutrient solution in the preparation of a product having the effect of promoting the growth and development of rice.

[0026] In an embodiment, the product includes culture solution, fertilizer and pesticide.

[0027] In an embodiment, the product is used to improve the growth and development of rice under acid rain, saline-alkali, drought, high temperature, low temperature, disease, strong light or a combination of the above conditions. The "combination" can be understood as the superposition of at least any two or more of the above conditions.

[0028] In an embodiment, the rice is indica rice "Yu Zhen Xiang".

[0029] It can be understood that the indicators of the growth and development of the rice provided by the present application include but are not limited to plant height, root length, fresh weight, dry weight, tiller number, nitrogen content, chlorophyll content, chlorophyll a content and chlorophyll b content.

[0030] On the other hand, the present application also provides a method for promoting the growth and development of rice "Yu Zhen Xiang", which comprises: culturing the seeds of rice "Yu Zhen Xiang" to the three-leaf stage, applying the nutrient solution, and continuing to culture to the whole growth period.

[0031] In an embodiment, the three-leaf stage rice seedlings are obtained by the following method: water culture of rice seeds to the appearance of the first incomplete leaf; replacing the water with ordinary rice nutrient solution to obtain three-leaf stage rice seedlings. The ordinary rice nutrient solution can be a nutrient solution having substantially the same composition as the nutrient solution provided by the present application, but the nitrogen element is only inorganic nitrogen.

[0032] The present application has at least the following beneficial effects: (1) The present application is aimed at the high-quality but growth and development prone to be hindered rice variety "Yu Zhen Xiang", and provides a nutrient solution capable of significantly improving the growth and development effect thereof, wherein the total nitrogen concentration is kept unchanged in the commonly used ordinary nutrient solution, part of inorganic nitrogen is replaced by amino acid organic nitrogen, and it is found that the nutrient solution obtained by using specific amino acid and concentration combination can effectively promote the planting effect of "Yu Zhen Xiang", and can significantly improve the biomass, plant height, tiller number, nitrogen content and chlorophyll content of "Yu Zhen Xiang" rice plants.

[0033] (2) In the process of developing the nutrient solution, different nutrient solutions and small molecule nutrient substances are set to simulate the growth of "Yu Zhen Xiang" in different regions, and it is found that specific amino acid and concentration combination can improve the adaptability of "Yu Zhen Xiang", thereby it is expected to solve the problem of planting effect of "Yu Zhen Xiang" in different ecological regions, and accelerate the process of factory seedling raising, realize the large-scale, intensive planting management and large-area planting of "Yu Zhen Xiang". BRIEF DESCRIPTION OF DRAWINGS

[0034] The accompanying drawings, which are incorporated into and form part of the specification, illustrate embodiments consistent with the present application and, together with the specification, serve to explain the principles of the application.

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced below. Those skilled in the art can also obtain other drawings according to these drawings without creative labor. In the drawings: Figures 1-3 The phenotypic pictures (A) of "Yu Zhen Xiang" plants under different treatment conditions in Example 1, and the statistical results of plant height (B), root length (C), fresh weight (D), tiller number (E) and dry weight (F), wherein the statistical results are expressed as mean ± SD, n = 10, and Duncan test is used for difference significance analysis, and the lower case letters are marked on the data, and in the same statistical graph, different lower case letters represent significant difference between them, p <0.05, the same lower case letter represents the same significance level; Figure 4 The single plant nitrogen percentage content test results of "Yu Zhen Xiang" in Example 2 (the statistical results are expressed as mean ± SD, n = 3; **, p <0.01; p <0.05); Figure 5The results of the chlorophyll content (A), chlorophyll a content (B), and chlorophyll b content (C) of "Yuzhenxiang" in Example 3 are shown in Table 1 (statistical results are expressed as mean ± SD, n = 3; **, <0.01; *, <0.05). p <0.01; *, p <0.05). DETAILED DESCRIPTION

[0036] The present application will be described in detail through specific embodiments. Unless otherwise specified, the technical means used in the present application are methods known to those skilled in the art. In addition, the embodiments should be understood as illustrative rather than limiting the scope of the present application, and the essence and protection scope of the present application are mainly defined by the claims. For those skilled in the art, various changes or modifications to the ingredients and amounts of the materials in these embodiments without departing from the essence and scope of the present application also fall within the protection scope of the present application.

[0037] In the following examples, unless otherwise specified, the reagents, instruments, consumables, etc. involved are commercially available. If no specific conditions are specified in the examples, the conventional conditions or the conditions recommended by the manufacturer are used.

[0038] In the present application, the rice variety is "Yuzhenxiang", a high-quality indica rice variety commonly planted in Meitan County, Zunyi City, Guizhou Province. The growth period is 155-156 days, it is a medium-late variety, the seeds are slender, and some grains have a top awn. The seed coat is golden yellow. The "Yuzhenxiang" seeds were collected in Meitan County, Zunyi City, Guizhou Province, and are now preserved in the Rice Industry Technology Research Institute of Guizhou University, and are disclosed in the following literature: Liu M. Study on the correlation of sensory quality of high-quality rice in Guizhou [D]. Guizhou University, 2018. The applicant promises to release the above-mentioned rice genetic resources to the public for 20 years from the date of application.

[0039] Example 1: Effect of different nutrient solution treatments on the growth and development of "Yuzhenxiang" The specific experimental process is as follows: Rice seed soaking: "Yuzhenxiang" rice seeds were soaked with 0.006% dilute nitric acid for 6-8 hours.

[0040] Rice seed culture: After soaking, the seeds were washed with deionized water for 3-5 times, divided into culture dishes, added with appropriate purified water, placed in an incubator at 37°C, and the water was changed 1-2 times daily.

[0041] Hydroponics: after the seed germination, the bud is grown to 1-2 cm, and then the seed is sown in a 96-hole plate and placed in a culture box with a capacity of 4 L of clear water culture, and the culture is carried out until the first incomplete leaf appears, and then the ordinary rice nutrient solution is replaced for culture until the 3-leaf stage, and then transplanted into a culture pot with a capacity of 15 L, and 12 L of rice nutrient solution is added into the culture pot, and 15 culture pots are set up, of which: 3 culture pots are set up as a single small molecule nutrient combination treatment group, 3 culture pots are set up as a single amino acid combination treatment group, 9 culture pots are set up as a complex treatment group of small molecule nutrient and amino acid combination, and 1 culture pot is set up as a control group.

[0042] The formula of the ordinary rice nutrient solution is the existing conventional culture solution, and the embodiment of the present application adopts the nutrient solution formula shown in Table 1 (reference literature: Yoshida S, Fomo DA, Cock JH, et al. Routine proceduce for growing rice plants in culture solution. In: Laboratory manual for physiological studies of rice [J]. International Rice Research Institute, 61-66.). At present, it is known that the conventional ordinary rice nutrient solution can meet the needs of rice growth, and the formula of the ordinary rice nutrient solution shown in Table 1 is only a specific presentation form of the ordinary rice nutrient solution.

[0043] Table 1 Formula of ordinary rice nutrient solution

[0044] The concentration of the prepared amino acid, small molecule nutrient and organic acid mother liquor is 1 mol / L, which is used for the preparation of the nutrient solution.

[0045] Among them, the nitrogen in the normal nutrient solution is inorganic nitrogen of ammonium salt and nitrate, and the final concentration is 2 mM, and the nutrient solution of the remaining experimental group is inorganic nitrogen with a final concentration of 1 mM, and the remaining 1 mM is achieved by adjusting the concentration of amino acid to achieve nitrogen balance. The chemical formula of arginine is C6H 14 N4O2, containing 4 nitrogen atoms; the chemical formula of alanine is C3H7NO2, containing 1 nitrogen atom; the chemical formula of phenylalanine is C9H 11 NO2, containing 1 nitrogen atom; the chemical formula of threonine is C4H9NO3, containing 1 nitrogen atom; the chemical formula of proline is C5H9NO2, containing 1 nitrogen atom; the chemical formula of glutamine is C5H 10 N2O3, containing 2 nitrogen atoms; the chemical formula of aspartic acid is C4H7NO4, containing 1 nitrogen atom; the chemical formula of tyrosine is C9H11 NO3 contains one nitrogen atom; cysteine ​​has the chemical formula C3H7NO2S and contains one nitrogen atom; glycine has the chemical formula C2H5NO2 and contains one nitrogen atom; asparagine has the chemical formula C4H8N2O3 and contains two nitrogen atoms; methionine has the chemical formula C5H... 11 NO2S contains one nitrogen atom; the chemical formula of tryptophan is C. 11 H 12 N2O2 contains two nitrogen atoms; serine has the chemical formula C3H7NO3 and contains one nitrogen atom.

[0046] The small molecule nutrients involved in this experiment include: Br, melatonin, γ-aminobutyric acid (GABA), salicylic acid, and vitamin C. By adding different combinations of small molecule nutrients at certain concentrations to the normal nutrient solution, in addition to supplementing the effect of nutrient elements, it can also be used to simulate the growth environment of different growth areas. For example: (1) The combination of GABA, salicylic acid, melatonin, and Br can be used to simulate the natural environment of acid rain, low temperature, and disease; (2) The combination of GABA, salicylic acid, melatonin, and vitamin C can be used to simulate the growth environment of high temperature, drought, and strong photo-oxidation; (3) The combination of GABA, salicylic acid, melatonin, vitamin C, and Br can be used to simulate the natural environment of stress such as low temperature, drought, disease, salinity, and strong light.

[0047] The specific details of the four groups in hydroponics (normal nutrient solution control group, single small molecule nutrient treatment group, small molecule nutrient treatment group, and amino acid combination and small molecule nutrient complex treatment group) are shown in Table 2: Table 2 Hydroponic Treatment Formulas and Concentrations

[0048] After cultivating the rice seedlings according to the above treatments, and once all the seedlings under each treatment of "Yuzhenxiang" had reached the tillering stage, the plant height, fresh weight, root length, dry weight, and number of tillers for each treatment were recorded. One seedling from each treatment with a plant height and number of tillers close to the average values ​​was selected and placed evenly on a black cloth in a row. The statistical results of plant phenotype, fresh weight, dry weight, plant height, root length, and number of tillers for "Yuzhenxiang" under each treatment condition are shown in Table 3. Figures 1-3 .

[0049] Table 3

[0050] From Table 3 and Figures 1-3From the results in Table 1, it can be seen that, except for Y3, Y5, Y7, Y9, Y12 and Y15, the growth and development of rice treated by the other groups were significantly better than CK or had no significant difference with CK, which indicated that there were great differences in the growth of "Yu Zhen Xiang" rice under different treatment conditions.

[0051] From the results of Y1-Y3 groups treated with small molecule nutrients alone, it can be seen that the small molecule nutrient combination added in Y1 in the ordinary nutrient solution had almost no difference with CK, Y2 was improved compared with CK, and the growth effect of Y3 group was lower than that of CK, which indicated that different small molecule nutrient combinations had significant effects on the growth of "Yu Zhen Xiang", and "Yu Zhen Xiang" also had great differences in adaptability to different growth environments. From the results of Y4-Y6 groups treated with amino acid combinations alone, it can be seen that Y4 had a significant promoting effect on the growth of "Yu Zhen Xiang", Y6 had a certain promoting effect, and the plant height of Y5 was lower than that of CK. It was speculated that the reason for the above results might be that there was a synergistic or antagonistic effect between different amino acid combinations. The synergistic effect could improve protein deposition rate, nutrient conversion and promote rice growth, while the antagonistic effect could lead to functional deficiency, even if the total protein was sufficient, it would also cause growth stagnation, metabolic disorder, and thus inhibit the growth of rice.

[0052] Further, from the results of Y7-Y15, it can be seen that adding small molecule nutrients to the nutrient solution containing amino acids does not necessarily have a more promoting effect on "Yu Zhen Xiang". For example, Y6, which has a certain promoting effect when used alone, obtained Y12 by adding the second group of small molecule nutrients, and all growth indicators were lower than CK. It was speculated that the reason for the above results might be that when only amino acids were added, the root system could mobilize all resources, efficiently activate and run the amino acid transport protein system. If small molecule nutrients are added on this basis, it will make the situation more complex, and thus may cause energy competition and inhibition of transport protein synthesis, thereby causing no obvious promoting effect or inhibiting the growth and development of "Yu Zhen Xiang".

[0053] Meanwhile, the above results also reflect that different amino acid combinations have a greater impact on the adaptability of "Yu Zhen Xiang" to different environments. Among them, although the amino acid combination of Y5 group has lower effect when used alone than the control group, but the promoting effect is obviously improved after adding different groups of small molecule nutrients, which shows that it is more suitable for improving the adaptability of "Yu Zhen Xiang". The amino acid combination of Y6 group has certain promoting effect when used alone, but the effect decreases after adding different groups of small molecule nutrients, which shows that it is more suitable for single addition. While the amino acid combination of Y4 group has most growth index results improved after adding each group of small molecule nutrients compared with using small molecule nutrients alone, which shows that it can improve the adaptability of "Yu Zhen Xiang" to drought, disease, salinity and other growth environments, and is conducive to improving its growth in different regions and expanding the planting area.

[0054] In summary, among the three groups of nutrient solutions containing different amino acid combinations designed in the present application, the amino acid combination of Y6 group is suitable for single application, the amino acid combination of Y5 group is suitable for complex application by adding small molecule nutrients, and the amino acid combination of Y4 group shows the best promoting effect on "Yu Zhen Xiang" when used alone and can also improve the adaptability of "Yu Zhen Xiang", which shows that the combination of arginine, alanine, phenylalanine, threonine and proline produces synergistic effect at a certain concentration, which is conducive to protein transport, root absorption and lignin synthesis, thereby significantly promoting the growth of "Yu Zhen Xiang" plants, and can be used as the best implementation mode.

[0055] Example 2: Effect of different treatments on the total nitrogen content percentage of "Yu Zhen Xiang" The rice materials of the present example are all from the above cultivation materials. The nitrogen content in the plant can be used as a physiological index to judge the growth and development of the plant. The measurement method of nitrogen content in rice plants is referred to the literature (Nanjing Agricultural College. Soil and agricultural chemical analysis. Beijing: Agricultural Press, 1980. 193-197).

[0056] Rice plant sampling: From the above organic nitrogen treatment group and inorganic nitrogen treatment group, select the treatments that promote and inhibit the growth and development of "Yu Zhen Xiang" more obviously (for example, Y3, Y4, Y7, Y8, Y11, Y12), plus the control (CK), a total of 7 treatments, take three rice plants for each treatment, place them in an 80°C oven to dry overnight, then take out the dried plants and divide them into leaves and stems, use a pulverizer to pulverize the samples into powder for plant nitrogen content determination. The plant nitrogen content data measured for each treatment sample is statistically plotted, and the results are shown in Figure 4

[0057] From Figure 4 ​The results can be known that the nitrogen content of rice plants treated by Y4 group is the highest, which shows that Y4 treatment is beneficial to improve the problem of poor growth and development in ordinary nutrient solution.

[0058] Example 3: Effect of different treatments on chlorophyll content in leaves of "Yuzhenxiang" The materials of rice in this example are all from the above culture materials. The chlorophyll content of rice leaves has certain correlation with photosynthetic rate and crop growth rate. In scientific research, chlorophyll content is often determined to represent the growth state of rice, and in production, leaf color change is often used as an important index for looking at seedlings and taking water and fertilizer measures. The measurement method of chlorophyll content in rice leaves is referred to the literature (Nurkaimaili Mulahti, Yang Yajie, Parhat Abudukerjimu, et al. Comparison of wheat chlorophyll content determination methods [J]. Jiangsu Agricultural Science, 2021, 49(09): 156-159.), in which the rice leaves are taken from the flag leaf part of the rice seedlings, and the leaves except the leaf veins are cut into 0.2-0.3 g fragments for detection. The measured chlorophyll content is statistically plotted, and the results are shown in Figure 5 .

[0059] From the results in Figure 5 , it can be known that the total chlorophyll content (A), chlorophyll a content (B), and chlorophyll b content (C) of "Yuzhenxiang" treated by Y4 group are all improved compared with the control, among which the total chlorophyll content is significantly improved. This shows that the amino acid combination of Y4 group used in ordinary nutrient solution can effectively promote the growth and development quality of "Yuzhenxiang". Figure 5 Figure 5 Figure 5

[0060] The above only describes the embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the scope of claims of the present application.​​​

Claims

1. A nutrient solution for promoting growth of rice "Yuzhenxiang", characterized in that, The concentration of nitrogen element in the nutrient solution is 1-3 mM, the nitrogen element is derived from inorganic nitrogen and organic nitrogen, and the organic nitrogen is selected from any one of the following amino acid combinations: Combination A: arginine, alanine, phenylalanine, threonine, proline; Combination B: glutamine, aspartic acid, tyrosine, cysteine, glycine; Combination C: asparagine, alanine, methionine, tryptophan, serine.

2. The nutritional liquid of claim 1, wherein, The concentration of amino acids in the combination A is: 0.1-0.5 mM arginine, 0.09-0.4 mM alanine, 0.08-0.3 mM phenylalanine, 0.08-0.5 mM threonine, 0.09-0.6 mM proline; and / or, The concentration of amino acids in the combination B is: 0.08-0.5 mM glutamine, 0.1-0.6 mM aspartic acid, 0.1-0.6 mM tyrosine, 0.05-0.5 mM cysteine, 0.08-0.5 mM glycine The concentration of amino acids in the combination C is: 0.1-0.6 mM asparagine, 0.05-0.5 mM alanine, 0.05-0.5 mM methionine, 0.05-0.5 mM tryptophan, 0.1-0.6 mM serine.

3. The nutritional liquid of claim 1, wherein, The molar ratio of inorganic nitrogen to organic nitrogen is (1-2):(2-1); and / or, The inorganic nitrogen is ammonium salt and / or nitrate.

4. The nutritional liquid of claim 1, wherein, The nutrient solution further comprises small molecule nutrients selected from any one or several of the following substances: GABA, salicylic acid, melatonin, vitamin C, Br; Preferably, the small molecule nutrients are combined with the amino acid combination of combination A or combination B.

5. The nutritional liquid of claim 1, wherein, The nutrient solution further comprises at least one inorganic salt containing any one of the following elements: phosphorus, potassium, sodium, calcium, magnesium, manganese, molybdenum, boron, zinc, copper, iron.

6. The nutritional liquid of claim 5, wherein, The nutrient solution further comprises at least one of the following substances: NaH2PO4•2H2O, K2SO4, CaCl2, MgSO4•7H2O, MnCl2•4H2O, Na2MoO4•2H2O, H3BO4, ZnSO4•7H2O, CuSO4•5H2O, EDTA-Na2, FeSO4•7H2O.

7. Use of the nutrient solution according to any one of claims 1-6 in the preparation of a product for promoting the growth and development of rice.

8. Use according to claim 7, characterized in that, The product includes culture solution, fertilizer, pesticide.

9. Use according to claim 7, characterized in that, The product is used to improve the growth and development of rice under acid rain, saline-alkali, drought, high temperature, low temperature, disease, strong light or complex environment.

10. A method for promoting the growth and development of rice "Yuzhenxiang", characterized in that, The method comprises: cultivating the seeds of rice "Yu Zhen Xiang" to the three-leaf stage, applying the nutrient solution according to any one of claims 1-6, and continuing to cultivate to the whole growth period.

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