Method and system for analyzing diversity of microorganisms in soil of traditional Chinese medicinal material planting land

Abstract

The invention discloses a traditional Chinese medicinal material planting land soil microbial diversity analysis method and system, and belongs to the field of soil detection and analysis, and the method comprises the following steps: when the content of effective components in target traditional Chinese medicinal materials in a target planting land is lower than a preset reference value, S1, carrying out microbial diversity analysis on the target planting land, microbial populations of the target planting land are obtained, and the soil pH value of the target planting land is detected; s2, comparing the microbial population of the target planting land with the microbial population of the genuine production area of the target traditional Chinese medicinal materials to obtain the microbial population difference between the target planting land and the genuine production area, and judging whether the soil pH value of the target planting land is within the pH value range of the genuine production area or not; s3, the soil environment of the target planting land is improved according to the microbial population difference and the pH value judgment result. Priori knowledge of a genuine production area can be fully utilized, a basis is provided for soil improvement of a traditional Chinese medicinal material planting field, and the growth quality of traditional Chinese medicinal materials is effectively improved.

Description

Technical Field

[0001] This invention belongs to the field of soil testing and analysis, and more specifically, relates to a method and system for analyzing soil microbial diversity in medicinal herb cultivation areas. Background Technology

[0002] The essence of Chinese medicinal herbs is natural plants, animals, and minerals. The accumulation of their effective components is greatly influenced by the local environment. Among them, the soil environment of the planting site directly affects the yield and quality of Chinese medicinal herbs. However, the excessive use of chemical fertilizers in the cultivation of Chinese medicinal herbs, leading to soil acidification and compaction, depletion of organic matter and trace elements, and changes in the microbial biodiversity ecology are important reasons. The problem of continuous cropping obstacles in the authentic producing areas and the substandard quality in non-authentic producing areas not only leads to unstable yields of Chinese medicinal herbs, but also results in the instability of the planting sites, making it difficult to guarantee the quality from the source.

[0003] Currently, relevant studies have proposed methods for improving the soil in medicinal herb planting areas. For example, patent application publication number CN115176550A proposes a method for improving the soil for medicinal herb planting using microbial fertilizers. This method involves sampling the soil in sections and randomly sampling the sampling points to measure various fertilizer indicators in the soil of the medicinal herb planting field. Based on the nutritional requirements of medicinal herb planting, the required content of microbial fertilizers in the soil is determined, allowing for targeted preparation and application of microbial fertilizers. This method improves the soil before medicinal herb cultivation by increasing the supply of nutrients through microorganisms, promoting the absorption and utilization of nutrients by medicinal herbs, reducing pests and diseases and increasing the yield of medicinal herbs, reducing the content of heavy metals in the soil, improving the soil acidity environment, improving soil compaction, and enhancing soil utilization.

[0004] However, this method improves the planting site based on the growth requirements of the medicinal herb varieties. The growth of Chinese medicinal herbs is a complex process involving the interaction of multiple factors. Directly mapping the growth requirements of medicinal herb varieties to relevant fertility indicators cannot effectively improve the growth quality of Chinese medicinal herbs. Summary of the Invention

[0005] To address the shortcomings and improvement needs of existing technologies, this invention provides a method and system for analyzing soil microbial diversity in medicinal herb planting areas. The purpose is to perceive the differences in the micro-ecological environment between medicinal herb planting areas and traditional medicinal herb producing areas through soil microbial diversity analysis, thereby making full use of prior knowledge of traditional medicinal herb producing areas, providing a basis for soil improvement in medicinal herb planting areas, and effectively improving the growth quality of medicinal herbs.

[0006] To achieve the above objectives, according to one aspect of the present invention, a method for analyzing soil microbial diversity in medicinal herb cultivation sites is provided, comprising: when the content of effective components in target medicinal herbs in the target cultivation site is lower than a preset reference value, performing the following steps: S1: Conduct microbial diversity analysis on the target planting site to obtain the microbial population of the target planting site and detect the soil pH value of the target planting site; S2: Compare the microbial population of the target planting site with the microbial population of the target Chinese medicinal material's authentic producing area to obtain the differences between the microbial populations of the target planting site and the authentic producing area, and determine whether the soil pH value of the target planting site is within the pH value range of the authentic producing area.

[0007] Furthermore, the method for analyzing soil microbial diversity in medicinal herb cultivation sites provided by this invention also includes: S3: Improve the soil environment of the target planting site based on the results of microbial population differences and pH value judgment.

[0008] Furthermore, in S3, if the differences in microbial populations include: the target planting area has missing or inferior microbial species compared to the authentic producing area, then the soil environment of the target planting area is improved, including: introducing microbial species that are missing or inferior compared to the authentic producing area into the target planting area.

[0009] Furthermore, in S3, if the differences in microbial populations include the presence of additional acidic soil characteristic bacteria in the target planting area compared to the authentic producing area, then the soil environment of the target planting area is improved, including: adjusting the soil pH value of the target planting area so that the soil pH value of the target planting area is within the pH value range of the authentic producing area, and after biodiversity analysis, the abundance of additional acidic soil characteristic bacteria in the target planting area compared to the authentic producing area is reduced to below a preset threshold.

[0010] Furthermore, in S3, if the differences in microbial populations also include the presence of additional cyanobacterial species in the target planting area compared to the authentic producing area, then the soil environment of the target planting area shall be improved, including optimizing the planting procedures to improve soil permeability.

[0011] According to another aspect of the present invention, a soil microbial diversity analysis system for medicinal herb planting sites is provided, comprising: an effective component detection device, a microbial diversity analysis device, a soil pH detection device, and an analysis and judgment device; Active ingredient detection equipment is used to detect the content of active ingredients in target Chinese medicinal materials from target planting areas; Microbial diversity analysis equipment is used to analyze the microbial diversity of a target planting site and obtain the microbial population of the target planting site; Soil pH testing equipment is used to test the soil pH value of the target planting site; The analysis and judgment equipment is used to compare the microbial population of the target planting area with the microbial population of the target Chinese medicinal material's authentic producing area when the content of the effective components in the target planting area is lower than the preset reference value. It obtains the difference between the microbial population of the target planting area and the authentic producing area, and determines whether the soil pH value of the target planting area is within the pH value range of the authentic producing area.

[0012] Furthermore, the soil microbial diversity analysis system for medicinal herb planting sites provided by the present invention also includes: soil improvement equipment; Soil improvement equipment is used to improve the soil environment of a target planting site based on the results of microbial population differences and pH value judgments.

[0013] Furthermore, the soil microbial diversity analysis system for medicinal herb planting sites provided by the present invention also includes: a GIS visualization system; The GIS visualization system is used to locate the target planting area and display a satellite map view of the target planting area.

[0014] Furthermore, the soil microbial diversity analysis system for medicinal herb planting sites provided by this invention also includes: a MySQL database and the SQLyog graphical management tool; The MySQL database is used to store the microbial population of the target planting area, the differences between the microbial population of the target planting area and the authentic producing area, and the corresponding soil improvement prompts. SQLyog is a graphical management tool that provides a visual interface for operating MySQL databases.

[0015] In summary, the above-described technical solutions conceived in this invention can achieve the following beneficial effects: (1) The soil microecological environment has a great influence on the quality of Chinese medicinal materials. The traditional producing areas of Chinese medicinal materials are the specific producing areas of the best quality medicinal materials. When the effective components of Chinese medicinal materials in the target planting area are lower than the preset reference value, this invention conducts microbial diversity analysis and pH detection on the soil of the target planting area. Based on this, the differences in microbial populations between the target planting area and the traditional producing area are analyzed, and whether the pH value of the soil in the target planting area is within the pH value range of the traditional producing area is analyzed. The comparative analysis results clearly show the differences in the microecological environment between the target planting area and the traditional producing area, providing a reliable basis for making full use of the prior knowledge of the traditional producing area and improving the soil to improve the quality of Chinese medicinal materials.

[0016] (2) After analyzing the differences in microbial populations between the target planting area and the authentic producing area, and whether the soil pH value of the target planting area is within the pH value range of the authentic producing area, the present invention further improves the soil environment of the target planting area based on the comparative analysis results, which can make full use of the prior knowledge of the authentic producing area and improve the quality of Chinese medicinal materials.

[0017] (3) The differences in microbial species between the target planting area and the authentic producing area analyzed in this invention include microbial species that are missing or inferior to those in the authentic producing area. When such differences are detected, microbial species that are missing or inferior to those in the authentic producing area will be introduced into the target planting area, thereby making the microbial population of the target planting area closer to that of the authentic producing area and improving the quality of Chinese medicinal materials cultivation in the target planting area.

[0018] (4) During the cultivation of Chinese medicinal herbs, the soil often becomes acidified due to excessive use of chemical fertilizers, which affects the micro-ecological environment of the soil and thus affects the quality of Chinese medicinal herb cultivation. The differences in microbial species between the target planting area and the authentic producing area analyzed in this invention also include the presence of additional acidic soil characteristic bacteria in the target planting area compared to the authentic producing area. When such differences occur, the pH value of the soil in the target planting area will be adjusted so that the pH value of the soil in the target planting area is within the pH value range of the authentic producing area, and the abundance of additional acidic soil characteristic bacteria in the target planting area will be reduced to below the threshold. This allows for timely detection of soil acidification and effective elimination of the impact of soil acidification on the micro-ecological environment, thereby further improving the quality of Chinese medicinal herb cultivation in the target planting area.

[0019] (5) In the process of planting Chinese medicinal materials, improper operation can also affect the soil micro-ecological environment. The differences in microbial species between the target planting area and the authentic producing area analyzed in this invention also include the presence of additional cyanobacteria in the target planting area compared to the authentic producing area. When such differences occur, the planting procedure will be optimized to improve soil permeability. This can avoid the serious decline of the effective components of medicinal materials due to poor soil drainage caused by improper planting, thereby further optimizing the soil micro-ecological environment of the target planting area and improving the quality of Chinese medicinal materials in the target planting area.

[0020] (6) The soil microbial diversity analysis system for Chinese medicinal herb planting sites provided by the present invention includes a GIS visualization system that enables bidirectional navigation between the planting site and the MySQL database, thereby intuitively displaying relevant information of a single plot within the reach of the GIS visualization system, providing a visual basis for solving planting problems. Attached Figure Description

[0021] Figure 1 A flowchart illustrating the method for analyzing soil microbial diversity in medicinal herb planting areas provided in this embodiment of the invention.

[0022] Figure 2 This is a schematic diagram illustrating the location of planting sites using a GIS visualization system, as described in an embodiment of the present invention.

[0023] Figure 3 This is a schematic diagram illustrating the delineation of planting areas using a GIS visualization system, as described in an embodiment of the present invention.

[0024] Figure 4 The image shows the results of microbial diversity analysis in Baokang area provided in this embodiment of the invention.

[0025] Figure 5 The image shows the results of microbial diversity analysis in Yingshan area provided in this embodiment of the invention.

[0026] Figure 6 A digital Alpha diversity index table provided for embodiments of the present invention.

[0027] Figure 7 The results of the risk analysis of soil microbial diversity in planting areas provided in the embodiments of the present invention. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Furthermore, the technical features involved in the various embodiments of this invention described below can be combined with each other as long as they do not conflict with each other.

[0029] In this invention, the terms "first," "second," etc. (if present) in the invention and the accompanying drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence.

[0030] To address the technical problem that existing technologies, which simply determine the microbial fertilizers applied to medicinal herb planting sites based on the growth requirements of medicinal herb varieties, neglect the importance of the authentic characteristics of medicinal herbs and thus fail to effectively improve the growth quality of medicinal herbs, this invention provides a method and system for analyzing soil microbial diversity in medicinal herb planting sites. The overall concept is to fully utilize prior knowledge of the authentic producing areas of medicinal herbs to study the differences in the micro-ecological environment of soil between authentic and non-authentic producing areas of the same medicinal herb, providing an effective basis for improving the micro-ecological environment of planting sites and enhancing the planting quality of medicinal herbs.

[0031] The ten traditional Chinese medicinal herbs of Hubei Province include: Artemisia argyi, Pinellia ternata, Gastrodia elata, Coptis chinensis, Poria cocos, Chrysanthemum morifolium, Atractylodes lancea, tortoise shell and turtle shell, Ginkgo biloba, Magnolia officinalis, and Polygonatum sibiricum (tied for tenth place). Among them, Atractylodes lancea is a rhizome-type medicinal herb, which faces challenges due to continuous cropping. Its authentic producing area is Yingshan County; Baokang County and Chibi area are not its authentic producing areas. Yingshan County is located in Huanggang City, in eastern Hubei; Baokang County is located in Xiangyang City, in northwestern Hubei; and Chibi City is located in Xianning City, in southern Hubei. These three areas are distributed in three different parts of Hubei and have excellent research value.

[0032] Without loss of generality, in the following embodiments, Atractylodes lancea is used as the target medicinal material for explanation and description, and the active ingredient in Atractylodes lancea is atractylodesin. It is readily understood that this invention is applicable not only to Atractylodes lancea, but also to all medicinal materials included in the Chinese Pharmacopoeia. Examples include: ginseng, Astragalus membranaceus, Panax notoginseng, Coptis chinensis, Codonopsis pilosula, Artemisia argyi, Glycyrrhiza uralensis, Lonicera japonica, Pinellia ternata, Polygonatum sibiricum, and Pseudostellaria heterophylla. When the target medicinal material is another medicinal material, the information such as the active ingredient and the authentic producing area of ​​the medicinal material can be determined accordingly.

[0033] The following is an example.

[0034] Example 1: A method for analyzing soil microbial diversity in medicinal herb cultivation sites, such as Figure 1 As shown, when the content of active ingredients in the target medicinal herbs at the target planting site is lower than a preset reference value, the following steps are performed: S1: Conduct microbial diversity analysis on the target planting site to obtain the microbial population of the target planting site and detect the soil pH value of the target planting site; S2: Compare the microbial population of the target planting site with the microbial population of the target Chinese medicinal material's authentic producing area to obtain the differences between the microbial populations of the target planting site and the authentic producing area, and determine whether the soil pH value of the target planting site is within the pH value range of the authentic producing area.

[0035] The soil microecological environment has a significant impact on the quality of Chinese medicinal herbs, and this environment is extremely complex. Blindly intervening in it may not effectively improve the quality of cultivated Chinese medicinal herbs. This embodiment is based on the content of active ingredients in Chinese medicinal herbs. Only when the content of active ingredients in the herbs is lower than a preset reference value is the soil microecological environment of the planting site analyzed and improved based on data from the authentic producing areas. The authentic producing areas of Chinese medicinal herbs are specific production areas of the highest quality herbs; using these as a reference can effectively improve the quality of cultivated herbs.

[0036] To effectively improve the cultivation quality of Chinese medicinal herbs, this embodiment, after analyzing the differences in microbial populations between the target planting area and the authentic producing area, and determining whether the soil pH value of the target planting area falls within the pH range of the authentic producing area, further includes: S3: Improve the soil environment of the target planting site based on the results of microbial population differences and pH value judgment.

[0037] Generally, the preset reference values ​​used to evaluate whether the content of effective components in Chinese medicinal materials meets the standards can be determined according to the Pharmacopoeia of the People's Republic of China (hereinafter referred to as the Pharmacopoeia). For example, according to the determination in Part I of the 2020 Pharmacopoeia (General Rule 0512 Method II), the content of atractylodes (C13H10O) on a dried basis shall not be less than 0.3%.

[0038] In practical applications, multiple authentic producing areas can be selected. Based on the analysis results of soil microbial diversity and soil pH measurement results of the selected authentic producing areas, the microbial population and pH range of the authentic producing areas can be determined.

[0039] In this embodiment, the target planting site is located in Baokang, and the selected authentic producing area is Yingshan. Five sampling points are set up in Baokang and Yingshan respectively. YS01~YS05 represent the five sampling points in Yingshan County, Hubei Province, and BK01~BK05 represent the sampling points in the five planting areas in Baokang County, Hubei Province.

[0040] During the sampling process, key information including sampling time, latitude and longitude, and coordinates was recorded. The coordinates of sampling point BK05 ​​(110.905780 E, 31.695751 N) were input into the GIS visualization system, and the system automatically redirected to a satellite map view, enabling precise location of the planting plot and displaying its full view. Figure 2 As shown, the land parcel is further delineated and coded in the software. The system attribute interface will display key information such as the parcel's coordinates, length, and area. Figure 3 As shown.

[0041] The results of the analysis of effective components of Chinese medicinal materials and soil at the ten sampling points YS01~YS05 and BK01~BK05 are shown in Table 1.

[0042]

[0043] As shown in Table 1, the atractylodes content at the five sampling points in Baokang, the target planting area, ranged from 0.06% to 0.23%, failing to meet the standards for traditional Chinese medicine decoction pieces. In contrast, the content at the five sampling points in Yingshan ranged from 0.32% to 0.91%, both exceeding the requirements of the pharmacopoeia. If the content of active ingredients in medicinal materials still fluctuates significantly after weight quantification, it will be difficult to meet the basic requirements for drug safety, efficacy, and quality stability.

[0044] As shown in Table 1, the pH range of the Yingshan sampling point in the authentic producing area is 6.5~6.9, which is generally suitable and conducive to the growth of medicinal materials. This pH range will be determined as the pH range of the authentic producing area. The pH range of the Baokang sampling point is 5.5~6.9. Among them, soil acidification is present in BK03 and BK05, and the acidification rate is as high as 40% in the five sampling points.

[0045] The results of microbial diversity analysis at each sampling point are shown in Table 2. Optionally, in this embodiment, the Alpha diversity index is selected to represent the results of soil microbial diversity analysis.

[0046]

[0047] As shown in Table 2, the abundance and Shannon index of BK03 were significantly lower than those of other sampling sites. The predicted depth of the sampling sites in both regions was greater than 0.9985, indicating that the sequencing depth had basically covered all species in the samples, and the detection results had extremely high reliability.

[0048] The visualization results of microbial diversity analysis at five sampling sites in Baokang area are as follows: Figure 4 As shown in the figure, the visualization results of microbial diversity analysis at five sampling sites in Yingshan area are as follows: Figure 5 As shown. Dilution curves randomly sample a certain number of sequences from a sample to predict the total number of species and their relative abundance at a given sequencing depth. Based on... Figure 4 and Figure 5 It can be seen that the BK03 curve at the Baokang sampling point deviates significantly from other sampling points, while the curves at the five sampling points in Yingshan are relatively close.

[0049] This embodiment compares the microbial populations of the target planting site with those of the authentic producing area of ​​the target Chinese medicinal herb, and determines whether the soil pH value of the target planting site is within the pH range of the authentic producing area. This accurately captures the differences in the micro-ecological environment between the target planting site and the authentic producing area. In this embodiment, the average abundance of species at the species level of Atractylodes lancea in Baokang area is shown in Table 3, and the average abundance of species at the species level of Atractylodes lancea in Yingshan area is shown in Table 4.

[0050]

[0051]

[0052] Optionally, in this embodiment, the relevant analysis results will be recorded in a MySQL database and displayed visually, such as... Figure 6 As shown.

[0053] In this embodiment, the differences in microbial populations between the target planting area and the authentic producing area include three categories, and in step S3 of this embodiment, corresponding soil improvement methods are proposed for each category of differences. Specifically: If the differences in microbial populations include the absence or inferiority of certain microbial species in the target planting area compared to the authentic producing area, then the soil environment of the target planting area is improved, including the introduction of microbial species that are absent or inferior to those in the authentic producing area into the target planting area. It is easy to understand that inferior species refer to species whose abundance in the target planting area is significantly lower than (the difference between the two is less than a certain threshold) in the authentic producing area. It should be noted that ideally, after introducing microbial species that are absent or inferior to those in the authentic producing area into the target planting area, there should be no missing or inferior microbial species in the target planting area compared to the authentic producing area. However, in reality, due to differences in the micro-ecological environment, some introduced species may not survive normally, resulting in the continued absence or inferiority of some species after introduction. Overall, this embodiment, by introducing microbial populations that are absent or inferior to those in the authentic producing area into the target planting area, can bring the microbial population of the target planting area closer to that of the authentic producing area, thereby improving the quality of medicinal herb cultivation in the target planting area. If the differences in microbial populations include the presence of additional acidic soil characteristic bacteria in the target planting area compared to the authentic producing area, then the soil environment of the target planting area will be improved. This includes adjusting the soil pH value of the target planting area to be within the pH range of the authentic producing area. Furthermore, after biodiversity analysis, the abundance of additional acidic soil characteristic bacteria in the target planting area compared to the authentic producing area will be reduced to below a preset threshold. This will enable timely detection of soil acidification and effectively eliminate the impact of soil acidification on the micro-ecological environment, thereby further improving the quality of medicinal herb cultivation in the target planting area. If the differences in microbial populations also include the presence of additional cyanobacterial species in the target planting area compared to the authentic producing area, then the soil environment of the target planting area should be improved, including optimizing the planting procedures to improve soil permeability. This can prevent poor soil drainage caused by improper planting, which could lead to a serious decline in the effective components of the medicinal materials, thereby further optimizing the soil micro-ecological environment of the target planting area and improving the quality of the Chinese medicinal materials in the target planting area.

[0054] In this embodiment, the microbial diversity analysis results of the target planting area and the authentic producing area show that there are seven types of bacteria that are missing in Baokang, the target planting area, compared with Yingshan, the authentic producing area. These include: (1) *Pseudomonas spp.*, a microorganism with environmental remediation function that can degrade organic pollutants and has been verified as an effective degrading bacteria; (2) *Massezia spp.*, a Gram-negative aerobic bacterium with short rod-shaped cells. This genus of microorganisms is widely distributed in various environments such as water, soil, plant rhizosphere and foliage, and has ecological functions such as participating in carbon and nitrogen cycling, wastewater denitrification, phosphorus dissolution and pollutant degradation; (3) *IMCC26256*; (4) *Ellin6067*; (5) *α-Proteobacteria*; (6) *Nocardia spp.*, a Gram-positive aerobic actinomycete. This type of bacteria plays a role in the carbon, nitrogen and sulfur cycle and participates in plant growth promotion activities; (7) *Dongxiuzhuyi*. These seven types of microorganisms are missing in the target planting area. These seven types of microorganisms need to be introduced into the target planting area to make the micro-ecological environment of the target planting area similar to that of the authentic producing area.

[0055] Based on the comparative analysis of microbial diversity between the target planting area and the authentic producing area, the data in Tables 3 and 4 show that among the coexisting fungal species in the two areas, some species (such as RB41) have significantly lower abundance in the target planting area and are at a relative disadvantage. It is also necessary to supplement these disadvantaged fungal species in the target planting area so that the micro-ecological environment of the target planting area is close to that of the authentic producing area.

[0056] The highest abundance of *Microsheathella* species was found at BK02 (3746), while it was almost undetectable at other sampling points. *Microsheathella* is a type of cyanobacteria widely found in shallow, still water such as ponds, ditches, and paddy fields, often epiphytic on aquatic plants or other objects. This indicates poor drainage at the BK02 planting site, which is detrimental to the growth of *Atractylodes lancea* rhizomes, resulting in the lowest concentration of active ingredients among all sampling points. As a rhizome-based medicinal herb, *Atractylodes lancea* should be planted using a raised bed method to maintain good soil aeration. The high abundance of *Microsheathella* in the soil suggests potential drainage problems, requiring corresponding improvements to planting procedures and soil aeration.

[0057] Comparative analysis of microbial diversity in the target planting area and the authentic producing area revealed that the abundance of Acidobacteriales was 7173 for BK03 and 2364 for BK05, with almost no detection at other sampling points. Acidobacteriales belong to the phylum Acidobacteria and are primarily chemoheterotrophic, obtaining energy by decomposing complex organic matter (such as plant polysaccharides and chitin), playing a crucial role in soil carbon cycling. This group typically adapts to acidic environments, with an optimal pH range of approximately 3.0–6.0, and is a key microbial group in acidic soil and sediment ecosystems. This result is consistent with conventional physicochemical testing: the pH at sampling point BK03 was 5.5, and that of BK05 ​​was 5.6. The high abundance of Acidobacteriales can serve as a bioindicator of soil acidification, reflecting that the site is no longer suitable for the healthy growth of medicinal herbs, requiring adjustment of soil pH and ensuring that the abundance of Acidobacteriales is reduced below a preset threshold. This preset threshold should ensure that the remaining Acidobacteriales do not affect the growth of medicinal herbs in the planting area.

[0058] The differences in the micro-ecological environment and corresponding soil improvement methods obtained from actual analysis will also be recorded in a MySQL database using the SQLyog graphical management tool and displayed visually, such as... Figure 7 As shown.

[0059] In summary, this embodiment constructs a visualization management system based on a GIS visualization system to detect the content of effective components of medicinal materials and the pH value of the physicochemical index of the soil at different sampling points in the authentic and non-authentic producing areas. Alpha diversity analysis is performed on the soil microorganisms at different sampling points in the authentic and non-authentic producing areas, and species composition analysis is performed on the soil microorganisms at different sampling points in the authentic and non-authentic producing areas. Based on these analysis and detection results, the differences in the micro-ecological environment between the target planting area and the authentic producing area are obtained. These differences reflect the risk of soil microbial diversity in the target planting area, providing an important basis for improving the soil micro-ecological environment.

[0060] Example 2: A soil microbial diversity analysis system for medicinal herb cultivation sites includes: active ingredient detection equipment, microbial diversity analysis equipment, soil pH detection equipment, analysis and judgment equipment, and soil improvement equipment; Active ingredient detection equipment is used to detect the content of active ingredients in target Chinese medicinal materials from target planting areas; Microbial diversity analysis equipment is used to analyze the microbial diversity of a target planting site and obtain the microbial population of the target planting site; Soil pH testing equipment is used to test the soil pH value of the target planting site; The analysis and judgment equipment is used to compare the microbial population of the target planting area with the microbial population of the target Chinese medicinal material's authentic producing area when the content of the effective components in the target planting area is lower than the preset reference value, to obtain the difference between the microbial population of the target planting area and the authentic producing area, and to determine whether the soil pH value of the target planting area is within the pH value range of the authentic producing area. Soil improvement equipment is used to improve the soil environment of a target planting site based on the results of microbial population differences and pH value judgments.

[0061] Furthermore, the soil microbial diversity analysis system for medicinal herb planting sites provided in this embodiment also includes: a GIS visualization system; a MySQL database and a supporting visualization tool, SQLyog.

[0062] The GIS visualization system is used to locate the target planting area and display a satellite map view of the target planting area.

[0063] In this embodiment, the MySQL database and the accompanying visual SQLyog graphical management tool are used to store the microbial population of the target planting area, the differences in microbial population between the target planting area and the authentic producing area, and the corresponding soil improvement prompts.

[0064] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for analyzing soil microbial diversity in medicinal herb cultivation sites, characterized in that, Comprise: When the content of effective components in the target Chinese medicinal material in the target planting site is lower than the preset reference value, the following steps are performed: S1: Perform microbial diversity analysis on the target planting site to obtain the microbial population of the target planting site, and detect the soil pH value of the target planting site; S2: Compare the microbial population of the target planting site with the microbial population of the genuine producing area of the target Chinese medicinal material to obtain the difference in microbial population between the target planting site and the genuine producing area, and determine whether the soil pH value of the target planting site is within the pH value range of the genuine producing area.

2. The method according to claim 1, wherein the method is characterized by, Further comprise: S3: Improve the soil environment of the target planting site according to the difference in microbial population and the pH value determination result.

3. The method according to claim 2, wherein the method is characterized by, In S3, if the difference in microbial population includes that the target planting site is missing or at a disadvantage compared to the genuine producing area, the soil environment of the target planting site is improved, including introducing the microbial species that are missing or at a disadvantage compared to the genuine producing area to the target planting site.

4. The method according to claim 2, wherein the method is characterized by, In S3, if the difference in microbial population includes that the target planting site has additional acid soil characteristic bacteria compared to the genuine producing area, the soil environment of the target planting site is improved, including adjusting the soil pH value of the target planting site to make the soil pH value of the target planting site within the pH value range of the genuine producing area, and after biological diversity analysis, the abundance of additional acid soil characteristic bacteria in the target planting site compared to the genuine producing area is below the preset threshold.

5. The method for analyzing soil microbial diversity of a Chinese medicinal material planting field according to claim 2, characterized in that, In S3, if the difference in microbial population also includes that the target planting site has additional cyanobacteria species compared to the genuine producing area, the soil environment of the target planting site is improved, including optimizing planting procedures to improve soil permeability.

6. A traditional Chinese medicinal material planting site soil microbial diversity analysis system, characterized in that, Comprise: Effective component detection equipment, microbial diversity analysis equipment, soil pH value detection equipment and analysis and judgment equipment; The effective component detection equipment is used to detect the content of effective components in the target Chinese medicinal material in the target planting site; The microbial diversity analysis equipment is used to perform microbial diversity analysis on the target planting site to obtain the microbial population of the target planting site; The soil pH value detection equipment is used to detect the soil pH value of the target planting site; The analysis and judgment equipment is used to compare the microbial population of the target planting site with the microbial population of the genuine producing area of the target Chinese medicinal material to obtain the difference in microbial population between the target planting site and the genuine producing area, and determine whether the soil pH value of the target planting site is within the pH value range of the genuine producing area when the content of effective components in the target Chinese medicinal material in the target planting site is lower than the preset reference value. 7.The system according to claim 6, characterized in that, Further comprise: Soil improvement equipment; The soil improvement equipment is used to improve the soil environment of the target planting site according to the difference in microbial population and the pH value determination result. 8.The system according to claim 6 or 7, characterized in that, Further comprise: GIS visualization system; The GIS visualization system is used to locate the target planting site and display the satellite map view of the target planting site. 9.The system according to claim 8, characterized in that, Also include: MySQL database and SQLyog graphical management tool; The MySQL database is used to store the microbial population of the target planting site, the difference between the microbial population of the target planting site and the genuine producing area, and the corresponding soil improvement prompt information; The SQLyog graphical management tool is used to provide a visual operation interface for operating the MySQL database.

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