Acaricidal composition and application thereof

Abstract

The invention belongs to the field of pesticide acaricides, and relates to an acaricidal composition and application thereof. The active components of the acaricidal composition comprise an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is any one of flutenzine and acequinocyl, and the mass ratio of the active component A to the active component B is 1: 27-18: 1. The composition disclosed by the invention has a synergistic effect on various pest mites which are harmful to agricultural production, the dosage of pesticides is reduced, the residues of the pesticides on crops are reduced, the environmental pollution is alleviated, the composition is safe to human and livestock and good in environmental compatibility, and the pest mites are not easy to generate drug resistance.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide acaricides, and particularly relates to application of an acaricidal composition in agricultural and forestry pests and sanitary pests. BACKGROUND

[0002] Fluensulfone has a chemical name of (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide and a chemical structural formula as shown in the following formula:

[0003]

[0004] In the process of agricultural production, the control of various types of pests mainly relies on the application of a large number of pesticide acaricides. The large and frequent unreasonable use of pesticide acaricides leads to the continuous enhancement of the resistance of pests, the more serious harm to crops, and the more difficult prevention and control. The mixing of two or more agents with different mechanisms can achieve the effects of treating different pests, improving the prevention and control effect of the agents, and reducing the development of pest resistance. The compound of formula (I) and fluensulfone or milbectin have obvious synergistic effects on the prevention and control of phytophagous spider mites, and the acaricidal composition and application of the compound of formula (I) and fluensulfone or milbectin have not been reported so far. SUMMARY

[0005] Based on the above situation, the present application aims to provide an acaricidal composition, which is mainly used for preventing and controlling pests in planting industry, especially phytophagous pests. The acaricidal composition or its preparation can enhance the efficacy, reduce the amount of pesticide, prolong the efficacy, and delay the development of resistance.

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows: an acaricidal composition, the active ingredients of which include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I):

[0007] The active ingredient B is any one of fluensulfone or milbectin;

[0008] Further, the mass ratio of the active ingredient A and the active ingredient B is 1:27-18:1.

[0009] Further, the mass ratio of the compound of formula (I) and fluensulfone is 1:27-18:1.

[0010] Further, the mass ratio of the compound of formula (I) and fluensulfone is 1:27, 1:25, 1:13, 2:23, 2:11, 1:5, 3:8, 9:5, 5:1, 14:1, or 18:1.

[0011] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25-18:1;

[0012] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25, 1:13, 2:23, 2:11, 1:5, 3:8, 9:5, 5:1, 14:1, 18:1;

[0013] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25-18:1;

[0014] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25, 1:13, 2:23, 2:11, 1:5, 3:8, 9:5, 5:1, 14:1, 18:1;

[0015] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25-18:1;

[0016] Further, the mass ratio of the compound of formula (I) to fluencarbaz is 1:25, 1:13, 2:23, 2:11, 1:5, 3:8, 9:5, 5:1, 14:1, 18:1;

[0017] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-80wt% based on 100wt% of the total weight of the acaricidal composition;

[0018] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-80wt% based on 100wt% of the total weight of the acaricidal composition;

[0019] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-80wt% based on 100wt% of the total weight of the acaricidal composition;

[0020] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-80wt% based on 100wt% of the total weight of the acaricidal composition;

[0021] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-80wt% based on 100wt% of the total weight of the acaricidal composition;

[0022] Further, the acaricidal composition comprises, in addition to the active ingredient, an agriculturally acceptable auxiliary ingredient selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0023] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl phthalate sulfonate sodium, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, ethylene glycol polyoxyethylene polyoxypropylene ether, fatty alcohol ethoxylate, isomeric alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, soap nut powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0024] Further, the dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, block polyether phosphate ester salt, arylphenol polyoxyethylene ether sulfate ester salt, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and / or

[0025] Further, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, dodecylbenzenesulfonic acid amine, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, benzylphenol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkylphenol ether phosphate and styrylphenol polyoxyethylene ether phosphate; and / or

[0026] Further, the thickening agent is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0027] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0028] Further, the antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0029] Further, the antifoaming agent is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oil, silicone compounds, C8-C10 One or more of the fatty alcohols; and / or

[0030] Further, the solvent is selected from one or more of benzene, toluene, xylene, trimethylbenzene, mesitylene, methanol, ethanol, isopropanol, n-butanol, isooctanol, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N,N-dimethyldecylamide, N-methylpyrrolidone, cyclohexanone, propylene carbonate, hydrocarbon carbonate, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0031] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate, isothiazolinones, benzimidazoles, iodopropyl groups, pyridinethiones, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0032] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0033] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether, synergistic amine; and / or

[0034] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0035] Furthermore, the acaricide composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from solid and / or liquid formulations;

[0036] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0037] Furthermore, the liquid formulation includes soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions;

[0038] Furthermore, the solid formulation is selected from wettable powders and water-dispersible granules; the liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, suspensions, suspension emulsions, microemulsions, and dispersible oil suspensions.

[0039] Furthermore, the solid dosage form is selected from wettable powders and water-dispersible granules;

[0040] Furthermore, the liquid formulation is selected from emulsifiable concentrates, water-in-oil emulsions, suspensions, suspensions, and microemulsions;

[0041] Furthermore, when the acaricide composition is a wettable powder, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-10 parts of dispersant, 1-5 parts of wetting agent, and filler to make up the balance; the wettable powder is prepared by pre-crushing and mixing the active ingredients and other additives evenly, and then pulverizing them with an air jet mill until the fineness meets the requirement of at least 98 wt% passing through a 45 μm test sieve to obtain the wettable powder product.

[0042] Furthermore, when the acaricide composition is a water-dispersible granule, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 3-20 parts of dispersant, 1-10 parts of wetting agent, 1-5 parts of disintegrant, and filler to make up the balance; the water-dispersible granule is prepared by adding the active ingredient to the carrier according to the formula ratio, adding surfactants and other functional additives thereto, mixing, and then adding 10-25% water after air jet milling, followed by kneading, granulation, drying, and sieving to obtain the water-dispersible granule product.

[0043] Furthermore, when the acaricide composition is an emulsifiable concentrate, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 5-30 parts of emulsifier, 0-5 parts of synergist, and the remainder is made up with solvent; the emulsifiable concentrate preparation method is to add the active ingredients to the solvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto, and stir and mix evenly in a mixing tank to obtain the emulsifiable concentrate product.

[0044] Further, when the acaricidal composition is an aqueous emulsion, the mass fractions of the components are: active ingredient A1-40 parts, active ingredient B1-40 parts, emulsifier 3-30 parts, solvent 5-50 parts, stabilizer 2-15 parts, antifreeze 1-5 parts, defoamer 0.1-8 parts, thickener 0.2-2 parts, and deionized water to make up the balance; the preparation method of the aqueous emulsion is as follows: according to the formulation ratio of the example, the active ingredient is dissolved in the solvent and the emulsifier is added to form a homogeneous oil phase; the deionized water and antifreeze are mixed together to form a homogeneous aqueous phase; the aqueous phase is added to the oil phase, stirred evenly, and then the defoamer is added and subjected to high-speed shearing to form a well-dispersed aqueous emulsion product.

[0045] Furthermore, when the acaricide composition is a suspension, the mass fractions of the components are: 1-40 parts of active ingredient A, 1-40 parts of active ingredient B, 1-15 parts of wetting and dispersing agent, 1-5 parts of antifreeze, 0.1-2 parts of thickener, 0.1-0.8 parts of defoamer, 0-10 parts of solvent, 0-5 parts of stabilizer, and deionized water to make up the balance; the suspension is prepared by placing the active ingredients, surfactants and other functional additives in a reaction vessel according to the formula ratio, adding water and mixing evenly, then subjecting it to high-speed shearing, wet milling, and finally homogenizing and filtering to obtain the suspension product.

[0046] Further, when the acaricidal composition is a suspension emulsion, the mass fractions of the components are: active ingredient A 1-40 parts, active ingredient B 1-40 parts, emulsifier 1-10 parts, dispersant 1-10 parts, antifreeze 1-5 parts, thickener 0.1-2 parts, defoamer 0.1-0.8 parts, solvent 1-40 parts, stabilizer 0-5 parts, and deionized water to make up the balance; the preparation method of this suspension emulsion is as follows: according to the formula ratio, active ingredient A is completely dissolved in the solvent, and an emulsifier is added to form an oil phase; active ingredient B, wetting and dispersing agent, antifreeze, defoamer, deionized water, etc. are wet-milled to D. 90 A suspension mother liquor with a particle size of less than 5 μm is prepared; after adding the oil phase to the suspension mother liquor and stirring evenly, a thickener is added and stirred and sheared evenly to prepare the suspension emulsion formulation of the composition of the present invention.

[0047] Further, when the acaricidal composition is a microemulsion, the mass fractions of the components are: active ingredient A1-40 parts, active ingredient B1-40 parts, emulsifier 8-30 parts, solvent 5-15 parts, stabilizer 2-15 parts, antifreeze 1-5 parts, defoamer 0.1-8 parts, and deionized water to make up the balance; the microemulsion preparation method is as follows: according to the formulation ratio of the example, the active ingredient is dissolved in the solvent and the emulsifier is added to form a homogeneous oil phase; the deionized water and antifreeze are mixed together to form a homogeneous aqueous phase; the aqueous phase is added to the oil phase, or the oil phase is added to the aqueous phase, and the mixture is stirred evenly to form a microemulsion product.

[0048] The present invention also discloses the application of the acaricidal composition described above in the prevention and control of harmful mites.

[0049] Furthermore, the mites mentioned are phytophagous mites;

[0050] Furthermore, the phytophagous mites are Panonychus citri McGregor, Eotetranychus kankitus (Ehara), Phyllocoptes oleivora, Tetranychus cinnabarinus (Boisduval), and Tetranychus urticae Koch.

[0051] Furthermore, the acaricidal composition and / or its formulation are applied at an effective dose to the mites that need to be controlled or the medium in which they grow.

[0052] The acaricide composition of the present invention has the following advantages:

[0053] 1) The acaricidal composition of the present invention exhibits a synergistic effect within a certain ratio range and can effectively control a variety of plant-eating mites;

[0054] 2) The two active ingredients in the acaricide composition of the present invention have different mechanisms of action, which effectively delays the development of drug resistance in harmful mites and extends the service life of the product;

[0055] 3) The acaricide composition of the present invention is environmentally friendly, safe and efficient, and its promotion and application have great economic and social benefits. Detailed Implementation

[0056] The present invention will be further illustrated below with reference to the embodiments. All percentages in the embodiments are by weight, but the present invention is not limited thereto. The content of the active ingredient in the composition of the present invention depends on the dosage when used alone, as well as on the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0057] Formulation preparation examples:

[0058] Preparation Example 1

[0059] 6% Formula (I) Compound Fluoxetine Emulsifiable Concentrate (1+5)

[0060] Formula composition: 1% of compound (Ⅰ), 5% of flufenoxuron, 15% of propylene carbonate, 3% of calcium dodecylbenzenesulfonate, 8% of castor oil polyoxyethylene ether, 1% of N,N-dimethylformamide, 2% of soybean oil, 25% of cyclohexanone, and methyl oleate to make up the balance.

[0061] Preparation method: First, add the active ingredient to the solvent and dissolve it completely. Then add the emulsifier and stir evenly to form a uniform and transparent oily liquid. Fill the container to prepare the emulsifiable concentrate of the composition of the present invention.

[0062] Preparation Example 2

[0063] 22% Formula (I) Compound Fluoxetine Suspension (6+16)

[0064] Formula composition: 6% of compound (I), 16% of flufenoxuron, 2% of alkyl naphthalene sulfonate formaldehyde condensate, 2% of sodium lignosulfonate, 4% of EO-PO block copolymer, 2.5% of naphthalene sulfonate, 0.5% of organosilicon defoamer, 0.1% of xanthan gum, 1.1% of magnesium aluminum silicate, 5% of ethylene glycol, 0.5% of sodium benzoate, and deionized water to make up the balance;

[0065] Preparation method: Add wetting and dispersing agent and defoamer to metered deionized water and stir evenly. Then add the active ingredient and stir evenly. Use zirconium beads and wet grind with a sand mill until D. 90 (90% of the particles have a particle size) < 5 μm to obtain a pulverized slurry. Thickener, antifreeze, and preservative are added to the pulverized slurry and mixed evenly. Deionized water is added to make up to 100%, and the mixture is sheared at high speed to obtain a suspension.

[0066] Preparation Example 3

[0067] 36% Formula (I) Compound Fluoxetine Suspension Emulsion (6+30)

[0068] Formula composition: 6% of compound (Ⅰ), 30% of flufenoxam, 1.5% of lignin sulfonate, 1% of polycarboxylate, 3% of tristyrylphenol ethoxylate phosphate, 3% of EO-PO block copolymer, 5% of ethylene glycol, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 0.5% of organosilicon defoamer, 10% of cyclohexanone, and deionized water to make up the balance;

[0069] Preparation method: According to the formula ratio, the compound of formula (Ⅰ) is completely solventized to form an oil phase. Fluoxetine, wetting and dispersing agent, antifreeze agent, defoamer, deionized water, etc. are wet-milled to D. 90 A suspension mother liquor with a particle size of less than 5μm is prepared; after adding the oil phase to the suspension mother liquor and stirring evenly, a thickener is added and stirred and sheared evenly to prepare the suspension emulsion product.

[0070] Preparation Example 4

[0071] 45% Formula (I) Compound Fluoxetine Water Dispersible Granules (15+30)

[0072] Formula composition: 15% of compound (Ⅰ), 30% of flufenoxuron, 8% of lignin sulfonate, 2% of α-olefin sulfonate, 8% of alkyl naphthalene sulfonate formaldehyde condensate, 1% of ammonium chloride, 1% of sodium carbonate, 10% of attapulgite, and the balance is made up of bentonite;

[0073] Preparation method: First, mix evenly, then pulverize to below 15μm using an air jet mill, mix evenly with water, granulate using a rotary granulator, dry at 60℃ until the moisture content is ≤3%, and sieve using 20-mesh and 60-mesh test sieves, discarding the large particles at the top and the dust filtered at the bottom to obtain water-dispersible granules.

[0074] Preparation Example 5:

[0075] 18% Formula (I) Compound·Acaricide Suspension (6+12)

[0076] Formula composition: 6% compound of formula (Ⅰ), 12% acaricide quinone, 3.5% polycarboxylate, 3% EO-PO block copolymer, 1.5% sodium dodecyl sulfate, 0.8% naphthalene sulfonate, 0.5% silicone defoamer, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.5% sodium benzoate, and deionized water to make up the balance;

[0077] Preparation method: According to the formula ratio, the compound of formula (I) is completely dissolved in a solvent to prepare an oil phase. The acaricide quinone, along with wetting and dispersing agents, antifreeze agents, defoamers, deionized water, etc., is wet-milled until D... 90 A suspension mother liquor with a particle size of less than 5μm is prepared; after adding the oil phase to the suspension mother liquor and stirring evenly, a thickener is added and stirred and sheared evenly to prepare the suspension product.

[0078] Preparation Example 6

[0079] 50% Formula (I) Compound · Acaricide Water Dispersible Granules (30+20)

[0080] Formula composition: 30% of compound (Ⅰ), 20% of acaricide quinone, 7.5% of lignin sulfonate, 2% of α-olefin sulfonate, 8% of alkyl naphthalene sulfonate formaldehyde condensate, 1% of sodium chloride, 1% of sodium carbonate, 12% of kaolin, and bentonite to make up the balance;

[0081] Preparation method: First, mix evenly, then pulverize to below 15μm using an air jet mill, mix evenly with water, granulate using a rotary granulator, dry at 60℃ until the moisture content is ≤3%, and sieve using 20-mesh and 60-mesh test sieves, discarding the large particles at the top and the dust filtered at the bottom to obtain water-dispersible granules.

[0082] Preparation Example 7

[0083] 20% Formula (I) Compound · Acaricide quinone soluble solution (10+10)

[0084] Formula composition: 10% of compound (Ⅰ), 10% of acaricide quinone, 3.5% of fatty amine polyoxyethylene ether, 15% of cyclohexanone, 5% of dimethyl sulfoxide, and N,N-dimethylformamide to make up the balance;

[0085] Preparation method: According to the formulation in the example, a homogeneous transparent liquid preparation composed of active ingredient, solvent, surfactant and antifreeze is diluted with water to form a true solution of active ingredient, thus obtaining a soluble product.

[0086] Preparation Example 8

[0087] 40% Formula (I) Compound·Acaricide Wettable Powder (24+16)

[0088] Formula composition: 24% of compound (I), 16% of acaricide, 5% of naphthalene sulfonate, 15% of sodium lignosulfonate, 1.5% of alkylphenol polyoxyethylene ether methyl ether condensate sulfate, 2.5% of sodium dodecyl sulfate, 10% of bentonite, and the balance is made up of kaolin.

[0089] Preparation method: First, pre-crush and mix evenly, then pulverize with an air jet mill until the fineness meets the requirement of at least 98 wt% passing through a 45 μm test sieve to obtain a wettable powder.

[0090] Indoor bioactivity assay

[0091] Indoor activity test 1

[0092] Test insect source: Citrus pseudomitrus;

[0093] Test reagents: 95% fenpropathrin technical grade, 95% flufenoxam technical grade, 96% compound of formula (I);

[0094] Test material preparation: Select second-instar nymphs that are kept indoors and have the same physiological condition. Cut double-sided tape into 2cm lengths and stick them to one end of a glass slide. Then select healthy mites and stick their backs onto the double-sided tape. Place 20 mites per slide and put them in a container lined with a damp sponge. Cover the container and place it at (25±1)℃. After 2 hours, examine the slide under a microscope, remove dead and injured individuals, and replenish the slide with 15 mites.

[0095] Reagent preparation: The original drug was prepared into a stock solution with acetone, and the stock solution was prepared into test reagents in different proportions with a 0.1% Tween 80 aqueous solution.

[0096] Drug treatment: Immerse the glass slide in the drug solution and gently shake for 5 seconds, then remove it, absorb the excess drug solution with absorbent paper, place it in a white porcelain dish with a damp sponge, cover it with a transparent plastic film, and keep it at (25±1)℃ for observation.

[0097] Experimental replication: Each treatment was repeated 4 times, with a blank control group consisting of a treatment without the drug.

[0098] Experimental investigation: 48 hours after treatment, the mortality of the test mites was checked, and the total number of mites and the number of dead mites were recorded. The mortality rate was calculated.

[0099]

[0100] In the formula:

[0101] P – Mortality rate, expressed as a percentage (%);

[0102] K represents the number of dead mites, measured in heads;

[0103] N represents the total number of mites treated, in units of heads.

[0104]

[0105] In the formula:

[0106] P1 – Corrected mortality rate, in percentage (%);

[0107] P t —The mortality rate is expressed as a percentage (%).

[0108] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0109] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the above formula; if the control mortality rate is >20%, the experiment needs to be repeated.

[0110] The LC of each drug was determined by linear regression analysis between the probability value of mortality and the logarithm of a series of concentrations (using the DPS data processing system). 50 The co-toxicity coefficient (CTC) of the mixture was calculated using the Sun Yunpei method to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0111]

[0112] In the formula:

[0113] ATI – Actual Measured Toxicity Index of Mixtures;

[0114] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0115] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0116] TTI = TI A *P A +TI B *P B

[0117] In the formula:

[0118] TTI – Theoretical Toxicity Index of Mixtures;

[0119] TIA —A. Toxicity index of drug A;

[0120] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0121] TI B —Toxicity index of drug B;

[0122] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0123]

[0124] In the formula:

[0125] CTC – Cotoxicity Coefficient;

[0126] ATI – Actual Measured Toxicity Index of Mixtures;

[0127] TTI – Theoretical Toxicity Index of Mixtures.

[0128] According to the criteria for classifying combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates an synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect.

[0129] Experimental results:

[0130] The combined toxicity assay of compound (I) with flufenoxam in different ratios against *Paecilomyces citrus* (nymphs): Table 1 shows the results of compound (I) against *Paecilomyces citrus* (nymphs) at different LC50 ratios. 50 The LC50 value for flufenoxuron against *Pachytomyces citrus* (nymphs) was 2.426 mg / L. 50 The value was 6.605 mg / L.

[0131] The compound of formula (I) exhibited an additive or synergistic effect in the control of citrus paronychia (nymphs) when the mass ratio of the compound of formula (I) to flufenoxuron was in the range of 1:27 to 18:1; the compound of formula (I) exhibited a synergistic effect in the control of citrus paronychia (nymphs) when the mass ratio of the compound of formula (I) to flufenoxuron was 3:8; the co-toxicity coefficient was 209.404, which was relatively high and the synergistic effect was significant.

[0132] Table 1 shows the results of indoor activity assays of compound (I) in combination with flufenoxuron against *Paecilomyces citrus* (nymphs).

[0133]

[0134]

[0135] Determination of the combined toxicity of compound (I) with different ratios of miticide to *Paecilomyces citrus* (nymphs):

[0136] Table 2 shows the LC50 results for miticide quinone against *Paecilomyces citrus* (nymphs). 50 The concentration was 1.687 mg / L. The control efficacy of compound (I) to miticide quinone in the range of 1:24 to 14:1 against citrus pterostigma (nymphs) showed an additive or synergistic effect. The control efficacy of compound (I) to miticide quinone in the range of 1:7 to 14:1 against citrus pterostigma (nymphs) showed a synergistic effect. When the mass ratio of compound (I) to miticide quinone was 3:2, the co-toxicity coefficient was 198.108, which was relatively high and the synergistic effect was significant.

[0137] Table 2 shows the results of indoor activity assays of compound (I) in combination with miticide quinone against *Paecilomyces citrus* (nymphs).

[0138]

[0139] Indoor activity test 2

[0140] Test insect source: Tetranychus cinnabarinus;

[0141] Test reagents: 95% fenpropathrin technical grade, 95% flufenoxam technical grade, 96% compound of formula (I);

[0142] Test material preparation: Select second-instar nymphs that are kept indoors and are in the same physiological state. Cut double-sided tape into 2cm lengths and attach them to one end of a glass slide. Then select healthy mites and stick their backs onto the double-sided tape, 20 mites per slide. Place them in a container lined with a damp sponge, cover the container, and place it at 25±1℃. After 2 hours, examine the slide under a microscope, remove any dead or injured individuals, and replenish the slide with 15 mites.

[0143] Reagent preparation: The original drug was prepared into a stock solution with acetone, and the stock solution was prepared into test reagents in different proportions with a 0.1% Tween 80 aqueous solution.

[0144] Drug treatment: Immerse the glass slide in the drug solution and gently shake for 5 seconds, then remove it, absorb the excess drug solution with absorbent paper, place it in a white porcelain dish with a damp sponge, cover it with a transparent plastic film, and keep it at (25±1)℃ for observation.

[0145] Experimental replication: Each treatment was repeated 4 times, with a blank control group consisting of a treatment without the drug.

[0146] Experimental investigation: 48 hours after treatment, the mortality of the test mites was checked, and the total number of mites and the number of dead mites were recorded. The mortality rate was calculated.

[0147]

[0148] In the formula:

[0149] P – Mortality rate, expressed as a percentage (%);

[0150] K represents the number of dead mites, measured in heads;

[0151] N represents the total number of mites treated, in units of heads.

[0152]

[0153] In the formula:

[0154] P1 – Corrected mortality rate, in percentage (%);

[0155] P t —The mortality rate is expressed as a percentage (%).

[0156] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0157] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the above formula; if the control mortality rate is >20%, the experiment needs to be repeated.

[0158] The LC of each drug was determined by linear regression analysis between the probability value of mortality and the logarithm of a series of concentrations (using the DPS data processing system). 50 The co-toxicity coefficient (CTC) of the mixture was calculated using the Sun Yunpei method to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0159]

[0160] In the formula:

[0161] ATI – Actual Measured Toxicity Index of Mixtures;

[0162] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0163] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0164] TTI = TI A *P A +TI B *P B

[0165] In the formula:

[0166] TTI – Theoretical Toxicity Index of Mixtures;

[0167] TI A—A. Toxicity index of drug A;

[0168] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0169] TI B —Toxicity index of drug B;

[0170] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0171]

[0172] In the formula:

[0173] CTC – Cotoxicity Coefficient;

[0174] ATI – Actual Measured Toxicity Index of Mixtures;

[0175] TTI – Theoretical Toxicity Index of Mixtures.

[0176] According to the criteria for classifying combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates an synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect.

[0177] Experimental results:

[0178] The combined toxicity assay of compound (I) with flufenoxam in different ratios against Tetranychus carmine (nymphs): Table 3 shows the results of compound (I) against Tetranychus carmine (nymphs) LC50. 50 The LC50 value for fluoxetine against Tetranychus carmine (nymphs) was 1.035 mg / L. 50 The value was 5.695 mg / L.

[0179] The compound of formula (I) exhibits a synergistic effect on the control of Tetranychus carmineus (nymphs) when the mass ratio of compound of formula (I) to flufenoxuron is in the range of 1:25 to 14:1. When the mass ratio of compound of formula (I) to flufenoxuron is 1:5, its co-toxicity coefficient is 200.341, which is relatively high and the synergistic effect is significant.

[0180] Table 3 shows the results of indoor activity assays of compound (I) in combination with fluoxetine against Tetranychus cinnabarinus nymphs.

[0181]

[0182]

[0183] The combined toxicity assay of compound (I) with different ratios of acaricide to Tetranychus cinnabarinus (nymphs): Table 4 shows the results of the combined toxicity assay of acaricide to Tetranychus cinnabarinus (nymphs).50 The value was 2.273 mg / L.

[0184] The compound of formula (I) exhibits a synergistic effect on the control of Tetranychus carmineus (nymphs) when the mass ratio of compound of formula (I) to miticide quinone is in the range of 1:20 to 9:1. When the mass ratio of compound of formula (I) to miticide quinone is 1:2, the co-toxicity coefficient is 210.229, which is relatively high and the synergistic effect is significant.

[0185] Table 4 shows the results of indoor activity assays of compound (I) in combination with acaricide quinone against Tetranychus cinnabarinus nymphs.

[0186]

[0187] Field efficacy examples

[0188] Field efficacy trial for controlling citrus red spider mites

[0189] Test crop: Citrus (Wogan);

[0190] Test subject: Citrus red spider mite;

[0191] Experimental design: 6 treatments, 3 replicates, 18 blocks, 2 trees per block, randomized block design, with one row of fruit trees between each block.

[0192] Table 5. Test reagents and dosages

[0193]

[0194] Experimental site: Citrus orchard in Shantou Village, Liuzhou City, Guangxi Zhuang Autonomous Region. The citrus trees in the experimental area are growing at a relatively uniform rate, and citrus red spider mite infestations have been severe in recent years. The soil fertility of the experimental site is high, and the cultivation and management conditions are good, with trees planted there year-round.

[0195] The pesticide was applied on September 26, 2022. The day of application was sunny with a high of 36°C and a low of 24°C. The wind was northerly at level 1. The weather was mostly cloudy during the trial period.

[0196] Application method, timing, and frequency: Use a motorized sprayer (WL-ABSC type) and manual spraying to ensure even spraying across the entire tree. Based on actual field conditions and farmers' water usage habits, determine the water usage per citrus tree to be 1.8L. The amount of pesticide applied should be such that the leaves are evenly covered with pesticide and a few drops drip down. Apply once.

[0197] Survey Method: Randomly select one point from each citrus tree along the east, west, south, north, and center directions. Mark one branch at each point and record the total number of live citrus red spider mites on the leaves of the marked branches (starting from the top leaves, with a minimum of 5 leaves). The survey mainly focuses on adult mites and nymphs (which can be observed with a magnifying glass).

[0198] Survey time and frequency: Surveys were conducted once before medication and once each at 3 days and 10 days after medication.

[0199] The efficacy of the drug is calculated using the following formula:

[0200]

[0201]

[0202] During the experiment, the citrus trees in each experimental plot grew well, and no pesticide damage was observed in any of the treatments.

[0203] Field trial results showed that:

[0204] As shown in Table 6, the combination of compound (I) with flufenoxuron and tebufenozide has a good control effect on citrus red spider mites, showing good rapid effect. Three days after application, the control efficacy of 22% compound (I)·flufenoxuron suspension (6+16) and 20% compound (I)·tebufenozide soluble concentrate (10+10) against citrus red spider mites was 92.80% and 91.04%, respectively. At the 0.05 level, through difference analysis, the mixture of compound (I) with flufenoxuron and tebufenozide was significantly higher than the control single agent.

[0205] Table 6. Control efficacy of different treatments for citrus red spider mite 3 days after application.

[0206]

[0207] Note: The efficacy (%) in the table is the average of each replicate, with the difference at the 5% level. The same applies to the following tables.

[0208] As shown in Table 7, with the passage of time, the combination of compound (I) with flufenoxuron and tebufenone increases the control efficacy and prolongs the duration of action against red spider mites on citrus trees. Ten days after application, the control efficacies of 22% compound (I)·flufenoxuron suspension (6+16) and 20% compound (I)·tebufenone soluble concentrate (10+10) were 94.83% and 94.70%, respectively. Through difference analysis, at the 0.05 level, the combination of compound (I) with flufenoxuron and tebufenone was significantly higher than the control single agent.

[0209] Table 7. Control efficacy of different treatments against citrus red spider mites 10 days after application.

[0210]

[0211] Note: The efficacy (%) in the table is the average of each replicate, with the difference at the 5% level. The same applies to the following tables.

[0212] Through indoor toxicity testing and field trials on citrus fruits, the acaricidal composition of the compound of formula (I) described in this invention, combined with any one of flufenoxuron or tebufenozide, showed good control effect against phytophagous mites.

[0213] The acaricide composition or formulation obtained by this invention exhibits significant control efficacy, superior to single agents in delaying the development of resistance and prolonging the duration of action. Furthermore, no phytotoxicity was observed in the experiments with the compounded agents, indicating that the enhanced synergistic effect of the obtained acaricide composition or formulation can reduce production and usage costs while ensuring crop safety.

[0214] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The active ingredients of the acaricidal composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I): Active ingredient B is either fluoxetine or fenbendazim.

2. The acaricide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:27 to 18:

1.

3. The acaricide composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to flufenoxuron is 1:27 to 18:1, and the mass ratio of the compound of formula (I) to acaricide is 1:24 to 14:

1. Preferably, the mass ratio of the compound of formula (I) to flufenoxuron is 1:25 to 18:1, and the mass ratio of the compound of formula (I) to acaricide is 1:20 to 14:

1.

4. The acaricide composition according to claim 1, characterized in that, Based on a total weight of 100 wt% for the acaricidal composition, the sum of the contents of active ingredient A and active ingredient B in the acaricidal composition is 1 to 80 wt%. Preferably, the sum of the contents of the compound of formula (I) and flufenoxuron in the acaricidal composition is 2-50 wt%; the sum of the contents of the compound of formula (I) and mitoxin in the acaricidal composition is 2-60 wt%.

5. The acaricide composition according to claim 1, characterized in that, In addition to the active ingredient, the acaricide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricide composition according to claim 5, characterized in that, The acaricide composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from solid and / or liquid formulations.

7. The acaricide composition according to claim 6, characterized in that, The solid formulation is selected from wettable powders and water-dispersible granules; the liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, suspensions, suspension emulsions, microemulsions, and dispersible oil suspensions.

8. The use of the acaricidal composition according to any one of claims 1-7 in the control of harmful mites.

9. The application according to claim 8, characterized in that, The mites mentioned are phytophagous mites; Preferably, the phytophagous mites are *Tetranychus citrus*, ...cinnabarinus*, and *Tetranychus two-spotted*.

10. The application according to claim 8, characterized in that, The acaricide composition and / or its formulation are applied at an effective dose to the mites that need to be controlled or to the medium in which they grow.

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