Compound biological enzyme preparation for dispelling effects of alcohol and preventing drunkenness and application thereof

Abstract

The invention discloses a compound biological enzyme preparation for dispelling effects of alcohol and preventing drunkenness and application thereof, and relates to the technical field of biology. The composite biological enzyme preparation comprises the following raw materials: 3-15 of papain, bromelain, alkaline protease, acid protease, pepsin, trypsin, alpha-amylase, lactase, lipase, cellulase, pectinase, glucoamylase, nuclease, nattokinase and serrapeptase. The composite biological enzyme preparation provided by the invention can obviously prolong the drunkenness latency period and greatly shorten the awakening time; the safety performance is excellent, the acute toxicity test shows that the compound meets the actual non-toxic level standard, and the oral administration safety is high. The alcohol effect dispelling and drunkenness preventing product developed by the invention has the core competitiveness of high efficiency, safety and strong adaptability, and has a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to a compound bioenzyme preparation for relieving hangovers and preventing intoxication, and its application. Background Technology

[0002] Currently, most hangover remedies and liver-protecting products on the market achieve their purpose through the following types of ingredients. One type is plant extracts, such as kudzu root, Japanese raisin tree fruit, turmeric, and vine tea extracts. These natural products are believed to have certain liver-protecting, bile-promoting, and alcohol metabolism-enhancing effects. However, their active ingredients are complex, their specific targets and mechanisms of action are often unclear, and their effects are relatively slow. Bioavailability also varies from person to person, resulting in significant individual differences in their effectiveness in preventing hangovers. The second type consists of various peptide ingredients, including corn oligopeptides, oyster peptides, and soybean peptides. These substances mainly exert their liver-protecting effect indirectly by providing the amino acids needed for liver repair, through antioxidant mechanisms, or by regulating immunity. Their ability to directly accelerate the clearance of ethanol and its toxic metabolites from the body is limited; therefore, they focus more on protecting and repairing the liver after drinking, rather than providing immediate hangover prevention and relief. The third category is probiotics, which utilize bacteria such as *Lactobacillus paracasei* and *Lactobacillus reuteri*. After colonizing the intestines, these probiotics express alcohol dehydrogenase and aldehyde dehydrogenase, thus achieving a hangover-relieving effect. However, this approach currently faces challenges due to the need for gene editing and repeated fermentation, resulting in a complex technical route. Overall, existing products based on plant extracts, peptides, and probiotics primarily function indirectly, often failing to provide rapid and effective hangover relief and prevention in cases of acute alcohol consumption.

[0003] With the development of biotechnology, bio-enzyme preparations, due to their high efficiency and specificity, have shown broad application prospects in the health field. In the specific area of ​​hangover relief and liver protection, some studies have begun to explore the application potential of bio-enzymes. For example, patent document CN109718255A mentions a method: first, *Acetobacter pasteurellii* VA2 is cultured in a solid culture medium containing yeast extract powder; then, the strain is inoculated into a liquid culture medium mixed with yeast extract powder and fermented alcohol and fermented; the fermented culture is then centrifuged and the supernatant is removed to obtain acetic acid bacteria; finally, excipients are mixed and dried to obtain a powder containing hangover-relieving enzymes. This technology reveals that it is feasible to obtain enzymes with hangover-relieving functions through microbial fermentation and suggests that bio-enzymes may have unique advantages in directly intervening in the alcohol metabolism process. This provides valuable insights for developing novel hangover relief and prevention products and confirms the development potential of bio-enzymes in this field.

[0004] While the value of bioenzymes in relieving and preventing hangovers has been initially recognized, current technologies largely focus on single enzymes or complex fermentation-based enzyme production, and their effectiveness and stability require further improvement. More importantly, there are currently no reports on the market of mature products utilizing carefully designed compound bioenzyme preparations as core active ingredients for relieving and preventing hangovers. This indicates that developing a compound bioenzyme preparation that can mimic or enhance the body's alcohol metabolism pathways, contains multiple highly efficient complementary enzymes, and possesses good oral stability will be an important direction for overcoming the limitations of existing hangover remedies. Such a preparation is expected to fundamentally utilize the highly efficient catalytic characteristics of bioenzymes to break down alcohol, alleviate hangover symptoms, and reduce the burden on the liver, meeting the market demand for efficient and rapid hangover prevention solutions. Summary of the Invention

[0005] The purpose of this invention is to provide a compound bio-enzyme preparation for relieving hangovers and preventing intoxication, and its application, to solve the problems existing in the prior art. This compound bio-enzyme preparation possesses excellent hangover-relieving and intoxication-preventing effects, providing a highly efficient, safe, and adaptable core bio-enzyme raw material for the development of hangover-relieving and intoxication-preventing products, and has broad application prospects.

[0006] To achieve the above objectives, the present invention provides the following solution: This invention provides a compound bio-enzyme preparation for relieving hangovers and preventing intoxication. The raw materials of the compound bio-enzyme preparation include 3-15 kinds selected from papain, bromelain, alkaline protease, acidic protease, pepsin, trypsin, α-amylase, lactase, lipase, cellulase, pectinase, glucosylamylase, nuclease, nattokinase, and sertrapeptidase.

[0007] Furthermore, the raw materials of the compound bio-enzyme preparation also include auxiliary components; The auxiliary ingredients are at least one of glucose, maltodextrin, taurine, soybean peptide powder, sodium alginate, lysine, vitamin C, and B vitamins.

[0008] Furthermore, the raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, lipase, cellulase and α-amylase.

[0009] Furthermore, the raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, α-amylase, glucosylamylase, and lactase.

[0010] Furthermore, the raw materials of the compound bio-enzyme preparation include acidic protease, α-amylase, lactase, lipase, cellulase, nattokinase, and sertrapeptidase.

[0011] Furthermore, the raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, and auxiliary components; The auxiliary ingredients include taurine, lysine, and B vitamins; The B vitamins mentioned are niacin, vitamin B6, and vitamin B12.

[0012] Furthermore, the raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, nuclease, taurine, and soybean peptide powder.

[0013] Furthermore, the compound bio-enzyme preparation comprises the following components in the following amounts: bromelain 25,000-35,000 IU / g, papain 15,000-25,000 IU / g, alkaline protease 45,000-55,000 IU / g, nuclease 100-300 IU / g, taurine 0.2-0.8 mg / g, and soybean peptide powder 10-50 mg / g.

[0014] The present invention also provides the application of the above-mentioned compound biological enzyme preparation in the preparation of products for relieving hangovers and / or preventing drunkenness.

[0015] The present invention also provides a product for relieving hangovers and / or preventing intoxication, comprising the above-mentioned compound biological enzyme preparation.

[0016] The present invention discloses the following technical effects: This invention develops a compound bio-enzyme preparation with excellent hangover relief and prevention effects. By precisely screening and scientifically compounding multiple enzymes, this invention constructs a synergistic compound bio-enzyme system, supplementing the body's endogenous metabolic pathways and efficiently decomposing ethanol and its toxic metabolites. It addresses the core pain points of traditional plant extract products, which have complex active ingredients, unclear mechanisms of action, slow onset of action, and significant individual variability; peptide products, which primarily focus on liver repair and struggle to directly remove alcohol toxicities; and probiotic products, which require colonization of the gut to function and suffer from poor shelf-life stability. This invention achieves the dual effects of rapid hangover relief and prevention in acute alcohol consumption scenarios. Furthermore, by introducing auxiliary ingredients such as taurine and B vitamins, this invention not only further enhances the hangover relief efficiency of the enzyme preparation but also supplements the nutrients needed for liver metabolism, reducing alcohol damage to the liver. Animal pharmacodynamic experiments have confirmed that the compound bio-enzyme preparation provided by this invention can significantly prolong the latency period of intoxication and greatly shorten the sobering-up time. The compound bio-enzyme preparation provided by this invention exhibits excellent safety performance; acute toxicity tests show that it meets the actual non-toxic level standard and has high oral safety.

[0017] The hangover remedy and anti-drunkenness product of this invention has the core competitiveness of high efficiency, safety, and strong adaptability, and has broad application prospects. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 The graph shows the changes in body weight of mice in different groups; Figure 2 The images show the morphological characteristics of organs and tissues in mice from different groups. Figure 3 This is a statistical graph showing the organ indices of mice in different groups under the maximum dose experiment; where AD represents the heart index, liver index, kidney index, and spleen index, respectively. Figure 4 A statistical graph showing the intoxication latency (A) and sobering time (B) in a mouse anti-drunkenness experiment. Detailed Implementation

[0020] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0022] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0023] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0024] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0025] Example 1 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 30,000 IU / g, papain 20,000 IU / g, alkaline protease 50,000 IU / g, lipase 5,000 IU / g, cellulase 140 IU / g, and glucoamylase 10,000 IU / g.

[0026] Example 2 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 25,000 IU / g, papain 25,000 IU / g, alkaline protease 45,000 IU / g, lipase 5,500 IU / g, cellulase 100 IU / g, and glucoamylase 15,000 IU / g.

[0027] Example 3 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 35,000 IU / g, papain 15,000 IU / g, alkaline protease 55,000 IU / g, lipase 4,500 IU / g, cellulase 200 IU / g, and glucoamylase 5,000 IU / g.

[0028] Example 4 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 30,000 IU / g, papain 20,000 IU / g, alkaline protease 50,000 IU / g, α-amylase 3,500 IU / g, and lactase 2,800 IU / g.

[0029] Example 5 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 25,000 IU / g, papain 25,000 IU / g, alkaline protease 45,000 IU / g, α-amylase 4,000 IU / g and lactase 2,400 IU / g.

[0030] Example 6 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 35,000 IU / g, papain 15,000 IU / g, alkaline protease 55,000 IU / g, α-amylase 3,000 IU / g and lactase 3,200 IU / g.

[0031] Example 7 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 30,000 IU / g, Papain 20,000 IU / g, Alkaline protease 50,000 IU / g, Taurine 0.5 mg / g, Lysine 0.2 mg / g, Niacin 0.5 mg / g, Vitamin B6 0.2 mg / g, and Vitamin B12 0.2 mg / 100 g.

[0032] Example 8 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 25,000 IU / g, Papain 25,000 IU / g, Alkaline protease 45,000 IU / g, Serapeptide 45,000 IU / g, Taurine 0.8 mg / g, Lysine 0.1 mg / g, Niacin 0.8 mg / g, Vitamin B6 0.1 mg / g, and Vitamin B12 0.3 mg / 100 g.

[0033] Example 9 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 35,000 IU / g, Papain 15,000 IU / g, Alkaline protease 55,000 IU / g, Taurine 0.2 mg / g, Lysine 0.3 mg / g, Niacin 0.2 mg / g, Vitamin B6 0.3 mg / g, and Vitamin B12 0.1 mg / 100 g.

[0034] Example 10 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Acidic protease 0.4 million IU / g, α-amylase 1.7 million IU / g, lactase 3,000 IU / g, lipase 800 IU / g and cellulase 140 IU / g.

[0035] Example 11 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Acidic protease 0.3 million IU / g, α-amylase 1.8 million IU / g, lactase 2500 IU / g, lipase 900 IU / g, cellulase 100 IU / g, and pepsin 100 IU / g.

[0036] Example 12 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: α-Amylase 16,000 IU / g, lactase 3,500 IU / g, lipase 700 IU / g, cellulase 180 IU / g and trypsin 5,000 IU / g.

[0037] Example 13 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 30,000 IU / g, papain 20,000 IU / g, alkaline protease 50,000 IU / g, nuclease 200 IU / g, taurine 0.5 mg / g, and soybean peptide powder 30 mg / g.

[0038] Example 14 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 25,000 IU / g, papain 25,000 IU / g, alkaline protease 45,000 IU / g, nuclease 300 IU / g, taurine 0.2 mg / g, and soybean peptide powder 50 mg / g.

[0039] Example 15 A compound enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 35,000 IU / g, papain 15,000 IU / g, alkaline protease 55,000 IU / g, nuclease 100 IU / g, taurine 0.8 mg / g, and soybean peptide powder 10 mg / g.

[0040] Example 16 A compound bio-enzyme preparation for relieving hangovers and preventing intoxication, with the following raw material formula: Bromelain 35,000 IU / g, papain 15,000 IU / g, alkaline protease 55,000 IU / g, nuclease 100 IU / g, nattokinase 2,500 IU / g, taurine 0.8 mg / g, and soybean peptide powder 10 mg / g.

[0041] Example 1 of effect verification Acute toxicity evaluation results (safety evaluation) of different formulations of compound biological enzyme preparations on normal mice: 1. Experimental Methods The maximum tolerated dose (MTD) test was used. Forty Kunming mice were randomly divided into four groups: a male control group, a female control group, a male administration group, and a female administration group. The control group was administered an equal volume of purified water by gavage. For the administration groups, the dosage and administration method were as follows: the designed dose was 15 g / kg b·wt. 15 g of the sample (the compound biological enzyme preparation of Example 13) was weighed and diluted with purified water to 40 mL to prepare the test substance, which was prepared immediately before use. After fasting for 12 hours with free access to water, the test sample was administered by gavage twice, 4 hours apart, with no food given during the interval. The gavage volume for each administration was 20 mg b·wt. Food was given 4 hours after the last gavage.

[0042] Observe and record the daily condition of the mice: After drug administration, observe the basic condition of the mice, including coat color, food intake, water intake, feces, daily activities, and mental state, for a period of 14 days. Determine whether toxic symptoms appear, and record the weight of the mice every other day from the date of the first administration. Dissect the mice to observe their tissues. 14 days after drug administration, mice in each group were euthanized by cervical dislocation and dissected to observe the pathological changes in their internal organs. If any abnormalities are visible to the naked eye, pathological examination is performed. After dissection, the heart, liver, kidney, and spleen tissues of the mice were weighed, and organ indices were calculated.

[0043] 2. Experimental Results 2.1 The mice were continuously observed after administration. No mice died after gavage administration of the compound bio-enzyme preparation at 15 g / kg. Mice in all groups showed reduced activity after gavage, exhibiting quietness and sluggishness. They responded to auditory stimuli, had normal appetite, smooth fur, and no abnormal secretions from the eyes, mouth, or nose. Their feces were normal. On the second day after administration, one female mouse experienced weight loss without other adverse reactions. Its weight recovered on the third day, which was considered to be due to the unpleasant odor of the compound bio-enzyme preparation, causing a short-term loss of appetite after gavage. The remaining mice were in normal condition, with no significant difference between the administered and control groups.

[0044] 2.2 Effects of compound biological enzyme preparations on changes in mouse body weight After gavage administration, mice in four groups were observed continuously for 14 days, and their body weight was recorded every other day. Body weight changes are shown in Table 1. Compared with the control group (female mice), there was no significant difference in body weight between the female mice in the treatment group and the control group (P>0.05); compared with the control group (male mice), there was no significant difference in body weight between the male mice in the treatment group and the control group (P>0.05). Throughout the experimental observation period, the body weight of both male and female mice continuously increased, and the increase was good, with a generally consistent trend. Figure 1 This indicates that administering the maximum dose of the compound bio-enzyme preparation to mice via gavage had no significant adverse effect on their body weight.

[0045] Table 1. Effects of gavage administration of compound biological enzyme preparations on body weight in mice. 2.3 Effects of compound biological enzyme preparations on organ indices in mice After dissecting the mice, the morphology of the organs and tissues of each group of mice was observed by macroscopic examination. It was found that there were no obvious abnormalities in the compound biological enzyme preparation group and the control group. Figure 2 The organ index is the ratio of the weight of a specific organ to the body weight of an experimental animal. Under normal circumstances, this ratio remains relatively constant. However, when an animal is exposed to toxins, the weight of the damaged organ can change, and the organ index will also change accordingly. In this experiment, the major organs of mice were weighed, and the organ coefficients were calculated. The results are shown in […]. Figure 3 There were no statistically significant differences in organ indices between the male administration group and the male control group of the compound bio-enzyme preparation (P>0.05), and no statistically significant differences in organ indices between the female administration group and the female control group of the compound bio-enzyme preparation (P>0.05). This suggests that the compound bio-enzyme preparation has low or essentially no toxicity.

[0046] 2.4 Evaluation results of acute toxicity of compound biological enzyme preparation Acute toxicity tests in mice showed no mortality after oral administration of the compound bio-enzyme preparation at concentrations of 15 g / kg and 20 mL / kg via gavage; therefore, the LD50 could not be calculated. 50 Value, median lethal dose (LD50) 50 LD50 is an important indicator for evaluating acute drug testing and can effectively assess the acute toxicity of drugs. 50 The higher the LD50, the lower the toxicity of the drug. The LD50 of the compound biological enzyme preparation was not detected in the experiment. 50 The values ​​indicate that the compound bio-enzyme preparation has very low toxicity. The results of this experiment show that its maximum dose (MTD) is greater than 15 g / kg. According to the acute toxicity grading standard, the above compound bio-enzyme preparation belongs to the practically non-toxic category.

[0047] Example 2 of effect verification Effects of compound biological enzyme preparations on the latency period and sobering time of alcohol intoxication in mice (pharmacological investigation): 1. Experimental Methods 1.1 Establishment of an alcohol-induced mouse model Based on the alcohol content of commonly available commercial baijiu, male Kunming mice (weighing 10-20 g) were acclimatized for one week and then administered 0.16 mL / 10 g of 56% ethanol by gavage to establish an alcohol intoxication model. The disappearance time and recovery time of the righting reflex were recorded for each group of mice. The standard for the disappearance time of the righting reflex was that the mouse remained supine for 30 seconds; otherwise, the righting reflex was considered to have recovered.

[0048] 1.2 Mouse grouping One hundred and twenty male Kunming mice were randomly divided into six groups: blank group, model group, positive control (Haiwang Jinzun tablets) group, enzyme formulation 1 group, enzyme formulation 2 group, and enzyme formulation 3 group, with 20 mice in each group. Ten mice were used in each group for anti-drunkenness and alcohol detoxification tests. Enzyme formulation 1 used the compound biological enzyme preparation prepared in Example 1; enzyme formulation 2 used the compound biological enzyme preparation prepared in Example 4; and enzyme formulation 3 used the compound biological enzyme preparation prepared in Example 13.

[0049] 1.3 Mouse grouping and sampling After fasting for 12 hours, the mice in the control group were given purified water by gavage.

[0050] Anti-drunkenness experiment: The positive control (Haiwang Jinzun tablets), enzyme formula 1, enzyme formula 2 and enzyme formula 3 groups were administered the positive control or the corresponding compound biological enzyme preparation by gavage, and ethanol was administered by gavage 30 minutes later.

[0051] Alcohol detoxification experiment: After 5 minutes of ethanol administration, the positive control (Haiwang Jinzun tablets) group, enzyme formula 1 group, enzyme formula 2 group and enzyme formula 3 group were administered the positive control or the corresponding compound biological enzyme preparation by gavage.

[0052] 1.4 Dosage The positive control (Haiwang Jinzun tablets) was prepared by grinding the tablets and dissolving them in water, and the oral dose for mice was 2.0 g / kg. The oral dose of the compound biological enzyme preparation was 0.25 g / kg, and the oral volume was 20 mL / kg.

[0053] 1.5 Detection Method Record the disappearance time and recovery time of the righting reflex for each group of mice. The standard for the disappearance time of the righting reflex is that the mouse remains supine for 30 seconds; otherwise, the righting reflex is considered to have recovered. Calculate the intoxication latency period (time from administration of alcohol to the disappearance of the righting reflex) and the sobering time (time from the disappearance of the righting reflex to the recovery of the righting reflex).

[0054] 2. Test Results: 2.1 Results of mouse anti-drunkenness experiment As shown in Table 2 and Figure 4As shown, compared with the model group, the latency period of intoxication was prolonged and the sobering time was shortened in all groups of mice. The experimental results showed that all groups of mice exhibited varying degrees of intoxication symptoms. However, one mouse in enzyme formulation 1 remained sober for more than 480 minutes, which may be because enzyme formulation 1 has the effect of prolonging the latency period of intoxication. However, the possibility that the dosage of 0.16 mL / 10 g of alcohol did not reach the level of intoxication due to large individual differences cannot be ruled out. In addition, statistical analysis showed that compared with the model group, the latency period of intoxication in enzyme formulation 3 was increased (P<0.05), and the sobering time was significantly shortened (P<0.01). Compared with the model group, the sobering time in enzyme formulation 2 was shortened (P<0.05). Compared with the positive control drug, the sobering time in enzyme formulation 5 was shortened (P<0.05).

[0055] Table 2 Results of mouse anti-drunkenness experiment Note: Preliminary experiments show that a dosage of 0.16 mL / 10 g of alcohol can achieve a 100% intoxication rate and a 20% mortality rate. In the table, the number of mice that died in the model group and the Haiwang Jinzun group were 2 and 8 respectively, while the number of mice that died in the enzyme formulation 3 group was 1 and 9 respectively. Compared with the model group, P < 0.05 indicates that the difference is statistically significant. P < 0.01 indicates a statistically significant difference; compared with the positive control drug, △ A p-value of <0.05 indicates that the difference is statistically significant.

[0056] 2.2 Results of the alcohol detoxification experiment in mice As shown in Table 3, compared with the model group, the latency period of intoxication was prolonged and the sobering-up time was shortened in all groups of mice. Compared with the model group, the latency period of intoxication in enzyme formulation 1 group was increased (P<0.05), and the sobering-up time was significantly shortened (P<0.01). Compared with the model group, the sobering-up time in enzyme formulation 2 group was shortened (P<0.05).

[0057] Table 3 Results of the alcohol detoxification experiment in mice Note: Preliminary experiments show that a dosage of 0.16 mL / 10 g of alcohol can achieve a 100% intoxication rate and a 20% mortality rate. In the table, the number of mice that died in the model group and enzyme formulation 1 group were 2 each, with 8 mice remaining. In the Haiwang Jinzun group and enzyme formulation 2 group, the number of mice that died was 1 each, with 9 mice remaining. Compared with the model group, P < 0.05 indicates that the difference is statistically significant. P<0.01 indicates that the difference is statistically significant.

[0058] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A compound biological enzyme preparation for relieving hangovers and preventing intoxication, characterized in that, The raw materials of the compound bio-enzyme preparation include 3-15 kinds of papain, bromelain, alkaline protease, acidic protease, pepsin, trypsin, α-amylase, lactase, lipase, cellulase, pectinase, glucosylamylase, nuclease, nattokinase and sertrapeptidase.

2. The compound biological enzyme preparation according to claim 1, characterized in that, The raw materials for the compound bio-enzyme preparation also include auxiliary components; The auxiliary ingredients are at least one of glucose, maltodextrin, taurine, soybean peptide powder, sodium alginate, lysine, vitamin C, and B vitamins.

3. The compound biological enzyme preparation according to claim 1, characterized in that, The raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, lipase, cellulase and α-amylase.

4. The compound biological enzyme preparation according to claim 1, characterized in that, The raw materials for the compound bio-enzyme preparation include papain, bromelain, alkaline protease, α-amylase, glucosylamylase, and lactase.

5. The compound biological enzyme preparation according to claim 1, characterized in that, The raw materials of the compound bio-enzyme preparation include acidic protease, α-amylase, lactase, lipase, cellulase, nattokinase, and sertraline peptide enzyme.

6. The compound biological enzyme preparation according to claim 2, characterized in that, The raw materials of the compound bio-enzyme preparation include papain, bromelain, acidic protease, and auxiliary components. The auxiliary ingredients include taurine, lysine, and B vitamins; The B vitamins mentioned are niacin, vitamin B6, and vitamin B12.

7. The compound biological enzyme preparation according to claim 2, characterized in that, The raw materials of the compound bio-enzyme preparation include papain, bromelain, alkaline protease, nuclease, taurine, and soybean peptide powder.

8. The compound biological enzyme preparation according to claim 7, characterized in that, The compound bio-enzyme preparation comprises the following components in the following amounts: bromelain 25,000-35,000 IU / g, papain 15,000-25,000 IU / g, alkaline protease 45,000-55,000 IU / g, nuclease 100-300 IU / g, taurine 0.2-0.8 mg / g, and soybean peptide powder 10-50 mg / g.

9. The use of a compound bio-enzyme preparation as described in any one of claims 1-8 in the preparation of products for relieving hangovers and / or preventing intoxication.

10. A product for relieving hangovers and / or preventing intoxication, characterized in that, Includes the compound biological enzyme preparation according to any one of claims 1-8.

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