Preparation process of fibroblasts

Abstract

The fibroblast preparation technology comprises a supporting plate, four supporting columns are fixedly connected to the top end of the supporting plate, an adjusting box is fixedly connected between the four supporting columns, an operation box is fixedly connected to the top end of the adjusting box, a telescopic opening is formed between the operation box and the adjusting box, and the telescopic opening is opened under power-on starting of a third motor. Under the power-on starting of second motors, the output end of one of the second motors drives the stirring auger to rotate, and the stirring auger or the cleaning frame is driven to rotate to drive the height adjusting mechanism to operate, so that the height of the preparation cylinder is increased until the stirring auger or the cleaning frame is completely immersed into the preparation cylinder; according to the preparation device, the stirring auger is arranged in the preparation cylinder, fibroblast preparation raw materials in the preparation cylinder are fully mixed, and the stirring auger can uniformly mix and stir at different height positions in the preparation cylinder through repeated operation of the height adjusting mechanism, so that the mixing efficiency during preparation of the fibroblast raw materials is improved.

Description

Technical Field

[0001] This invention relates to the field of preparation technology, and in particular to a process for preparing fibroblasts. Background Technology

[0002] Fibroblasts have spindle-shaped or irregularly triangular cell bodies with an oval nucleus in the center and cytoplasmic protrusions, growing radially. Besides true fibroblasts, tissues originating from mesodermal mesenchyme, such as cardiac muscle, smooth muscle, osteoblasts, and vascular endothelium, often exhibit this type of morphology. Furthermore, any cell in culture whose morphology resembles that of a fibroblast can be called a fibroblast. In the existing preparation of fibroblast cell bodies, the raw materials first need to be stirred and mixed, which requires the use of appropriate stirring equipment. However, the existing stirring equipment often results in insufficient mixing of the raw materials, which affects the quality of the preparation. Therefore, a new fibroblast preparation process is proposed. Summary of the Invention

[0003] The purpose of this invention is to provide a process for preparing fibroblasts, which solves the problem of poor mixing efficiency in the preparation of fibroblast raw materials in the prior art.

[0004] To achieve this objective, the present invention adopts the following technical solution: a fibroblast preparation process, comprising a support plate, four pillars fixedly connected to the top of the support plate, an adjustment box fixedly connected between the four pillars, an operation box fixedly connected to the top of the adjustment box, a telescopic opening provided between the operation box and the adjustment box, and a preparation cylinder installed inside the telescopic opening through a height adjustment mechanism; One end of the operating box is fixedly connected to a first motor, and the output end of the first motor is fixedly connected to a rotating screw. The other end of the rotating screw extends into the interior of the operating box and is rotatably connected to one end inside the operating box. A rotating sleeve is screwed onto the outer wall of the rotating screw, and a moving block is fixedly connected to the outer wall of the rotating sleeve. Two second motors are fixedly connected to the top of the moving block. The output ends of the two second motors extend to the bottom of the moving block and are respectively fixedly installed with a stirring auger and a cleaning frame. Cleaning brushes are fixedly connected to both ends of the cleaning frame. The height adjustment mechanism includes a third motor, one end of which is fixedly connected to the bottom end of the support plate. An adjusting screw is fixedly connected to the output end of the third motor. The top end of the adjusting screw extends to the top end of the support plate and is screwed with a push sleeve. Four push rods are fixedly connected to the top end of the push sleeve. A push block is fixedly connected between the top ends of the four push rods. An installation component is fixedly connected to the top end of the push block. An installation plate is fixedly installed on the top end of the installation component by screws. The top end of the installation plate is fixedly connected to the bottom end of the precast cylinder.

[0005] Preferably, one end of the movable block is fixedly connected to a limiting cylinder, and the inside of the limiting cylinder is slidably connected to a limiting rod, the two ends of which are respectively fixedly connected to the two ends inside the operating box.

[0006] Preferably, both ends of the push screw sleeve are fixedly connected to a limiting sleeve, and the inside of each of the two limiting sleeves is slidably connected to a limiting rod, the two ends of the limiting rod being fixedly connected to the top end of the support plate and the bottom end of the adjusting box, respectively.

[0007] Preferably, stable feet are fixedly connected to the four corners of the bottom end of the support plate and the four corners of the bottom end of the control box.

[0008] Preferably, an observation window is provided at one end of the operation box and at one end of the adjustment box, and a tempered glass observation window is fixedly installed inside the observation window.

[0009] Preferably, both ends of the pushing block are fixedly connected to limiting blocks, and both ends of the adjustment box are provided with limiting slots, with the two limiting blocks respectively slidingly connected to the interior of the two limiting slots.

[0010] Compared with the prior art, the present invention has the following beneficial effects: This fibroblast preparation process involves pouring the fibroblast preparation raw materials into the preparation cylinder. Upon energizing a first motor, the output of the first motor drives a rotating screw to rotate, which in turn moves a rotating sleeve via the screw thread. During this movement, the rotating sleeve moves a moving block until the center point of the stirring auger or cleaning frame is aligned with the center point of the preparation cylinder. Then, upon energizing a third motor, a height adjustment mechanism is activated, raising the height of the preparation cylinder until the stirring auger or cleaning frame is completely submerged. Upon energizing a second motor, one of the second motors drives the stirring auger to rotate, thoroughly mixing the fibroblast preparation raw materials inside the preparation cylinder. Through repeated operation of the height adjustment mechanism, the stirring auger can perform uniform mixing at different heights within the preparation cylinder, improving the mixing efficiency during fibroblast preparation. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] The structures, proportions, sizes, etc., shown in the accompanying drawings of this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed in the specification, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0013] Figure 1 A frontal view of a fibroblast preparation process; Figure 2 A frontal cross-sectional view of a fibroblast preparation process; Figure 3 This is a frontal cross-sectional view of the internal structure of a fibroblast preparation process control box. Figure 4 This is a frontal cross-sectional schematic diagram of a height adjustment mechanism for a fibroblast preparation process.

[0014] Illustration: 1. Support plate; 2. Support column; 3. Adjustment box; 4. Control box; 5. Preparation cylinder; 6. First motor; 7. Rotating screw; 8. Rotating sleeve; 9. Moving block; 10. Second motor; 11. Stirring auger; 12. Cleaning frame; 13. Cleaning brush; 14. Third motor; 15. Adjustment screw; 16. Push sleeve; 17. Push rod; 18. Push block; 19. Mounting component; 20. Mounting plate; 21. Limiting cylinder; 22. Limiting rod; 23. Limiting sleeve; 24. Limiting rod; 25. Stabilizing foot; 26. Observation window; 27. Limiting block. Detailed Implementation

[0015] To make the objectives, features, and advantages of this invention more apparent and understandable, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0016] In the description of this invention, it should be understood that the terms "upper," "lower," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. It should be noted that when a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be a component positioned centrally in the connection.

[0017] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0018] This invention provides a process for preparing fibroblasts, including a support plate 1. Four pillars 2 are fixedly connected to the top of the support plate 1. An adjustment box 3 is fixedly connected between the four pillars 2. An operation box 4 is fixedly connected to the top of the adjustment box 3. A telescopic opening is provided between the operation box 4 and the adjustment box 3. A preparation cylinder 5 is installed inside the telescopic opening via a height adjustment mechanism. The height adjustment mechanism includes a third motor 14. One end of the third motor 14 is fixedly connected to the bottom end of the support plate 1. An adjustment screw 15 is fixedly connected to the output end of the third motor 14. The top end of the adjustment screw 15 extends to the top of the support plate 1 and is screwed with a pusher sleeve 16. Four pushers are fixedly connected to the top end of the pusher sleeve 16. A push block 18 is fixedly connected to the top of each of the four push rods 17. A mounting piece 19 is fixedly connected to the top of each push block 18. A mounting plate 20 is fixedly mounted to the top of each mounting piece 19 by screws. The top of the mounting plate 20 is fixedly connected to the bottom of the precast cylinder. When the third motor 14 is powered on, it drives the adjusting screw 15 to rotate. As the adjusting screw 15 rotates, it drives the push screw sleeve 16 to move. When the push screw sleeve 16 moves, it pushes the preparation cylinder 5 through the cooperation of the push rods 17 and the push blocks 18, raising the height of the preparation cylinder 5 until the stirring auger 11 or cleaning frame 12 is completely submerged inside the preparation cylinder 5. When the second motor 10 is powered on, its output drives the stirring auger 11 to rotate, thoroughly mixing the fibroblast preparation materials inside the preparation cylinder 5. Through repeated operation of the height adjustment mechanism, the stirring auger 11 can uniformly mix the materials at different heights inside the preparation cylinder 5. One end of the operation box 4 is fixedly connected to the first motor 6, and the output end of the first motor 6 is fixedly connected to a rotating screw 7. The other end of the rotating screw 7 extends into the operation box 4 and is rotatably connected to one end inside the operation box 4. A rotating screw sleeve 8 is screwed onto the outer wall of the rotating screw 7, and a moving block 9 is fixedly connected to the outer wall of the rotating screw sleeve 8. Two second motors 10 are fixedly connected to the top of block 9. The output ends of the two second motors 10 extend to the bottom of the moving block 9 and are respectively fixedly installed with a stirring auger 11 and a cleaning frame 12. Cleaning brushes 13 are fixedly connected to both ends of the cleaning frame 12. After the fibroblast preparation raw material is poured into the preparation cylinder 5, the output end of the first motor 6 will drive the rotating screw 7 to rotate and drive the rotating sleeve 8 to move through the thread. During the movement, the rotating sleeve 8 will drive the moving block 9 to move until the center point of the stirring auger 11 or the cleaning frame 12 is aligned with the center point of the preparation cylinder 5. It should also be noted that one end of the moving block 9 is fixedly connected to a limiting cylinder 21, and a limiting rod 22 is slidably connected inside the limiting cylinder 21. Both ends of the limiting rod 22 are fixedly connected to both ends inside the operating box 4. When the rotating screw sleeve 8 moves the moving block 9, the limiting cylinder 21 and the limiting rod 22 improve the stability of the moving block 9. Both ends of the pushing screw sleeve 16 are fixedly connected to limiting sleeves 23, and both limiting sleeves 23 are slidably connected to limiting rods 24. Both ends of the limiting rods 24 are fixedly connected to the top of the support plate 1 and the bottom of the adjusting box 3, respectively. When the pushing screw sleeve 16 moves, the limiting sleeves 23 and the limiting rods 24 improve the stability of the pushing screw sleeve 16. To improve efficiency, stable feet 25 are fixedly connected to the four corners of the bottom of the support plate 1 and the four corners of the bottom of the operation box 4 to support the position of the overall device and increase its height. Observation windows 26 are opened at one end of the operation box 4 and one end of the adjustment box 3. Observation tempered glass is fixedly installed inside the observation windows 26 to allow staff to clean and observe the preparation process. Limiting blocks 27 are fixedly connected to both ends of the push block 18. Limiting slots are opened at both ends inside the adjustment box 3. The two limiting blocks 27 are slidably connected to the inside of the two limiting slots. When the push screw sleeve 16 drives the push block 18 to move, the movement of the limiting blocks 27 inside the limiting slots improves the stability of the push block 18.

[0019] In summary, in practical use, the fibroblast preparation process involves first pouring the fibroblast preparation raw materials into the preparation cylinder 5. Then, after the first motor 6 is energized, its output drives the rotating screw 7 to rotate, which in turn moves the rotating sleeve 8 via its thread. During this movement, the rotating sleeve 8 moves the moving block 9 until the center point of the stirring auger 11 or cleaning frame 12 is aligned with the center point of the preparation cylinder 5. Finally, the third motor 14 is energized, driving the adjusting screw 15 to rotate. As the adjusting screw 15 rotates via its thread... The pusher sleeve 16 is moved, and when it moves, it pushes the preparation cylinder 5 with the cooperation of the push rod 17 and the push block 18, raising the height of the preparation cylinder 5 until the stirring auger 11 or the cleaning frame 12 is completely submerged inside the preparation cylinder 5. When the second motor 10 is powered on, the output end of one of the second motors 10 drives the stirring auger 11 to rotate, which fully mixes the fibroblast preparation raw materials inside the preparation cylinder 5. Through the repeated operation of the height adjustment mechanism, the stirring auger 11 can evenly mix and stir at different heights inside the preparation cylinder 5.

[0020] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A process for preparing fibroblasts, comprising branch plates (1), characterized in that, The top of the support plate (1) is fixedly connected to four pillars (2), and an adjustment box (3) is fixedly connected between the four pillars (2). The top of the adjustment box (3) is fixedly connected to an operation box (4). A telescopic opening is provided between the operation box (4) and the adjustment box (3). A preparation cylinder (5) is installed inside the telescopic opening through a height adjustment mechanism. One end of the operation box (4) is fixedly connected to a first motor (6), and the output end of the first motor (6) is fixedly connected to a rotating screw (7). The other end of the rotating screw (7) extends into the interior of the operation box (4) and is rotatably connected to one end inside the operation box (4). A rotating sleeve (8) is screwed onto the outer wall of the rotating screw (7). A moving block (9) is fixedly connected to the outer wall of the rotating sleeve (8). Two second motors (10) are fixedly connected to the top of the moving block (9). The output ends of the two second motors (10) extend to the bottom end of the moving block (9) and are respectively fixedly installed with a stirring auger (11) and a cleaning frame (12). Cleaning brushes (13) are fixedly connected to both ends of the cleaning frame (12). The height adjustment mechanism includes a third motor (14), one end of which is fixedly connected to the bottom end of the support plate (1). An adjusting screw (15) is fixedly connected to the output end of the third motor (14). The top end of the adjusting screw (15) extends to the top end of the support plate (1) and is screwed with a push sleeve (16). Four push rods (17) are fixedly connected to the top end of the push sleeve (16). Push blocks (18) are fixedly connected between the top ends of the four push rods (17). An installation part (19) is fixedly connected to the top end of the push block (18). An installation plate (20) is fixedly installed on the top end of the installation part (19) by screws. The top end of the installation plate (20) is fixedly connected to the bottom end of the precast cylinder.

2. The fibroblast preparation process according to claim 1, characterized in that, One end of the moving block (9) is fixedly connected to a limiting cylinder (21), and the inside of the limiting cylinder (21) is slidably connected to a limiting rod (22). The two ends of the limiting rod (22) are respectively fixedly connected to the two ends inside the operating box (4).

3. The fibroblast preparation process according to claim 1, characterized in that, Both ends of the push screw sleeve (16) are fixedly connected to the limiting sleeve (23), and the two limiting sleeves (23) are slidably connected to the limiting rod (24). The two ends of the limiting rod (24) are fixedly connected to the top of the support plate (1) and the bottom of the adjustment box (3), respectively.

4. The fibroblast preparation process according to claim 1, characterized in that, Stable feet (25) are fixedly connected to the four corners of the bottom end of the support plate (1) and the four corners of the bottom end of the operation box (4).

5. The fibroblast preparation process according to claim 1, characterized in that, An observation window (26) is provided at one end of the operation box (4) and at one end of the adjustment box (3), and a tempered glass observation glass is fixedly installed inside the observation window (26).

6. The fibroblast preparation process according to claim 1, characterized in that, Both ends of the push block (18) are fixedly connected to the limiting block (27), and both ends of the adjustment box (3) are provided with limiting slots. The two limiting blocks (27) are slidably connected to the interior of the two limiting slots respectively.

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