Sorting device and sorting method thereof
By leveraging the synergistic effect of the sheath flow channel and the electromagnetic drive assembly, flow cytometry cell sorting is achieved by generating shock waves through the vibration of the sheath fluid and magnetic field. This solves the noise problem of the gas valve structure, improves the accuracy and efficiency of sorting, and reduces noise interference.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-24
- Publication Date
- 2026-04-10
AI Technical Summary
In existing flow cytometry cell sorting technology, the frequent opening and closing of the gas valve structure and the release of high-pressure gas cause noise interference to the experimental environment and impose an auditory burden on the operators.
By combining a sheath flow channel and an electromagnetic drive assembly, non-target particles are controlled to flow into the first flow channel through the sheath fluid. The electromagnetic drive assembly applies a magnetic field to the magnet in the injection chamber, causing the magnet to vibrate and generate a shock wave that is transmitted to the target particles, thus achieving sorting. This eliminates the need for frequent opening and closing of the gas valve structure and the release of high-pressure gas.
This reduces noise interference during the operation of the sorting device, alleviates the auditory burden on operators, and improves the accuracy and efficiency of the sorting operation.
Smart Images
Figure CN121825701A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of microfluidics, and particularly relates to a sorting device and a sorting method thereof. BACKGROUND
[0002] Cell sorting technology such as flow cytometry sorting is an indispensable core technology in medical research, which can identify, separate and obtain target cell subpopulations from a complex cell population according to specific physical or biochemical characteristics.
[0003] In related technologies, the flow cytometry sorting technology realizes the screening of target cells through liquid flow disturbance generated by a gas valve structure. However, the gas valve structure will generate a large noise during the working process due to frequent opening and closing and high-pressure gas release, which will interfere with the experimental environment and also cause a certain degree of auditory burden to the operator. SUMMARY
[0004] The present application aims to at least solve one of the above technical problems in the prior art. To this end, the purpose of the present application is to provide a sorting device, which does not need the frequent opening and closing of the gas valve structure and the release of high-pressure gas in the sorting process of target particles, can reduce the noise generated by the sorting device during work, reduce the interference of noise on the experimental environment, and thus reduce the auditory burden of the operator.
[0005] The present application also proposes a sorting method based on the above sorting device.
[0006] The sorting device according to the first aspect of the present application comprises: The sorting assembly comprises a sheath flow channel, a sorting flow channel and a conductive blind end, the sheath flow channel is in communication with the sorting flow channel, the sheath flow channel is used for flowing sheath liquid, the sheath liquid contains non-target particles and target particles, the sorting flow channel comprises a first flow channel and a second flow channel, the sheath liquid can control the non-target particles to flow into the first flow channel, the conductive blind end is located at the inlet end of the sorting flow channel, a perfusion cavity is arranged below the conductive blind end, and a magnetically susceptible body is arranged in the perfusion cavity; The electromagnetic driving assembly is located directly above the sorting assembly, the electromagnetic driving assembly can apply a magnetic field to the magnetically susceptible body, the magnetically susceptible body can vibrate to generate a shock wave according to the magnetic field, and the conductive blind end can transmit the shock wave to the target particles to control the target particles to flow into the second flow channel.
[0007] The sorting device according to the embodiments of this application has at least the following beneficial effects: Non-target particles are controlled to flow into the first flow channel by the sheath fluid in the sheath flow channel, and a magnetic field is applied to the magnet in the injection chamber using an electromagnetic drive component. This causes the magnet to vibrate with the magnetic field, generating a shock wave, which is then transmitted to the target particles through a conduction blind end, thereby directing the target particles into the second flow channel and completing the sorting operation. The sorting process for target particles in this application eliminates the need for frequent opening and closing of the gas valve structure and the release of high-pressure gas, reducing noise generated during the operation of the sorting device and minimizing noise interference with the experimental environment, thus reducing the auditory burden on operators. Simultaneously, the sheath fluid pressure regulation in the sheath flow channel, in coordination with the electromagnetic drive component, enables the sorting of non-target particles and target particles, ensuring the accuracy and reliability of the sorting operation and improving sorting efficiency.
[0008] According to some embodiments of this application, a thin film is disposed between the infusion cavity and the conduction blind end, and the thin film can undergo elastic deformation to generate the shock wave.
[0009] According to some embodiments of this application, the sorting component further includes a main channel, which is connected to the sorting channel, and the main channel is used to allow the non-target particles and the target particles to flow and be sorted.
[0010] According to some embodiments of this application, the sheath flow channel includes a first sheath flow channel and a second sheath flow channel, and the first sheath flow channel and the second sheath flow channel are disposed on both sides of the main flow channel along the axial direction of the main flow channel; Along the axial direction of the main flow channel, the first sheath flow channel and the second sheath flow channel are located on the same side of the main flow channel, and the sheath fluid pressure in the first sheath flow channel is less than the sheath fluid pressure in the second sheath flow channel.
[0011] According to some embodiments of this application, the sorting device further includes a laser detection component, the detection end of which is located in the main channel, and the laser detection component is used to detect the target particles to activate the electromagnetic drive component.
[0012] According to some embodiments of this application, the magnetized body is configured as a magnetic fluid, a magnetized metal powder, or a magnet block.
[0013] The sorting method according to the second aspect of this application, using the sorting apparatus according to the first aspect of this application, includes the following steps: Processing particles to be sorted; wherein, the particles to be sorted include the non-target particles and the target particles; The sample solution is injected into the main channel of the sorting device; Adjust the sheath fluid pressure and the sample fluid injection pressure within the sheath flow channel to allow the non-target particles to flow into the first flow channel; When the laser detection component of the sorting device detects the target particle, the electromagnetic drive component is activated. The electromagnetic drive component applies a magnetic field to the magnetized body, and the magnetized body vibrates with the magnetic field to generate a shock wave. The conduction blind end transmits the shock wave to the target particle to control the target particle to flow into the second flow channel.
[0014] The sorting method according to the embodiments of this application has at least the following beneficial effects: By adjusting the sheath fluid pressure in the sheath flow channel, non-target particles are directed into the first flow channel. Combined with the detection of target particles by the laser detection component triggering the electromagnetic drive component to start, the shock wave generated by the vibration of the magnet is transmitted to the target particle through the conduction blind end to control the target particle to flow into the second flow channel. The entire target particle sorting process does not require frequent opening and closing of the gas valve structure and release of high-pressure gas, which can reduce noise and reduce the interference of noise on the experimental environment, thereby reducing the auditory burden on the operator and improving the environmental comfort of the sorting operation. At the same time, the sheath fluid pressure adjustment in the sheath flow channel and the electromagnetic drive component work together to achieve the sorting of non-target particles and target particles, ensuring the accuracy and reliability of the sorting operation and improving the sorting efficiency.
[0015] According to some embodiments of this application, the electromagnetic drive assembly applies a magnetic field to the magnetized body, including: The magnetic force exerted by the electromagnetic drive component on the magnetized body is continuously increased until the target particle flows into the second flow channel.
[0016] According to some embodiments of this application, the electromagnetic drive assembly applies a magnetic field to the magnetized body, including: The startup time of the electromagnetic drive component is continuously increased so that the shock wave continues to act on the target particle until it flows into the second flow channel.
[0017] According to some embodiments of this application, the laser detection component of the sorting device detects the target particles, including: The position of the laser detection component is adjusted according to the delay time of the sorting device and the flow rate of the particles to be sorted, so that when the electromagnetic drive component is started, the particles to be sorted are located at the inlet end of the sorting channel.
[0018] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description
[0019] The present application will be further described below with reference to the accompanying drawings and embodiments, wherein: Figure 1 This is a schematic diagram of the sorting device according to an embodiment of this application; Figure 2 This is a flowchart illustrating the sorting method of an embodiment of this application.
[0020] Reference numerals: 100, sorting component; 110, sheath flow channel; 111, first sheath flow channel; 112, second sheath flow channel; 120, sorting channel; 121, first flow channel; 122, second flow channel; 130, conduction blind end; 140, infusion chamber; 150, main flow channel; 200, laser detection line. Detailed Implementation
[0021] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.
[0022] In the description of this application, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0023] In the description of this application, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.
[0024] In the description of this application, unless otherwise expressly defined, terms such as "setup," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this application in conjunction with the specific content of the technical solution.
[0025] In the description of this application, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0026] Reference Figure 1 The first aspect of this application provides a sorting device, including a sorting component 100 and an electromagnetic drive component. The sorting component 100 includes a sheath flow channel 110, a sorting channel 120, and a conduction blind end 130. The sheath flow channel 110 is connected to the sorting channel 120 and is used to supply sheath fluid flow. The sheath fluid is used to drive the sample fluid flow. The sample fluid contains non-target particles and target particles. The sorting channel 120 includes a first channel 121 and a second channel 122. The sheath fluid can control the flow of non-target particles into the first channel 121. The conduction blind end 130 is located at the inlet end of the sorting channel 120. A perfusion chamber 140 is provided below the conduction blind end 130, and a magnet is provided in the perfusion chamber 140. The electromagnetic drive component is located directly above the sorting component 100. The electromagnetic drive component can apply a magnetic field to the magnet. The magnet can vibrate with the magnetic field to generate a shock wave. The conduction blind end 130 can transmit the shock wave to the target particles to control the flow of the target particles into the second channel 122.
[0027] Specifically, non-target particles are controlled to flow into the first flow channel 121 by the sheath fluid in the sheath flow channel 110, and a magnetic field is applied to the magnet in the injection chamber 140 using an electromagnetic drive component. The magnet vibrates with the magnetic field, generating a shock wave, which is then transmitted to the target particles through the conduction blind end 130, thereby causing the target particles to flow into the second flow channel 122, thus completing the sorting operation. This application eliminates the need for frequent opening and closing of gas valves and high-pressure gas release during the target particle sorting process, reducing noise generated during operation and minimizing noise interference with the experimental environment, thereby reducing the auditory burden on operators. Simultaneously, the sheath fluid pressure regulation in the sheath flow channel 110, in coordination with the electromagnetic drive component, enables the sorting of non-target and target particles, ensuring the accuracy and reliability of the sorting operation and improving sorting efficiency.
[0028] It should be noted that using an electromagnetic drive assembly to apply a magnetic field to the magnet inside the infusion cavity 140 is existing technology, and this application has not made any improvements to this part, so its structure and principle will not be described in detail.
[0029] In some embodiments, a thin film (not shown) is disposed between the infusion chamber 140 and the conduction blind end 130. The thin film can undergo elastic deformation to generate a shock wave. Specifically, after the electromagnetic drive assembly is activated to generate a magnetic field, the magnetized body in the infusion chamber 140 is moved upward under the action of the magnetic field, thereby causing the thin film to undergo elastic deformation. Based on its own elastic restoring force, the thin film can generate a shock wave and act on the conduction blind end 130. The shock wave acts on the target particles through the conduction blind end 130. By cooperating with the electromagnetic drive assembly, the magnetized body, and the thin film, the sorting efficiency of the particles to be sorted can be improved compared with the existing method of sorting target particles by using liquid flow disturbance generated by a gas valve structure.
[0030] It should be noted that the particles to be sorted include non-target particles and target particles. The target particles are marked with fluorescent signals, which facilitates the sorting device to identify and sort them. This part is existing technology, and this application has not made any improvements to this part, so its principle and process will not be described in detail.
[0031] In some embodiments, the film is made of one of the following materials: silicone, PDMS (polydimethylsiloxane), COC (cyclic olefin copolymer), COP (cyclic olefin polymer), PS (polystyrene), and PC (polycarbonate). This ensures that the film can undergo elastic deformation in response to changes in the magnetic field when driven by a magnet, generating a shock wave and transmitting it to the conductive blind end 130. Of course, in actual design, the material of the film can be designed according to actual needs.
[0032] In some embodiments, the thickness of the film is 1 μm to 100 μm. In actual design, the thickness of the film can be designed according to actual needs.
[0033] In some embodiments, the magnetized material within the infusion chamber 140 is configured as a magnetic fluid, magnetized metal powder, or a magnet block, which can meet the differentiated requirements for the response speed and vibration intensity of the magnetized material in different application scenarios. Of course, in actual design, the structure of the magnetized material can be designed according to actual needs. In addition, compared with the magnetized material within the infusion chamber 140 being configured as a magnetic fluid or magnetized metal powder, when the magnetized material is configured as a magnet block, the magnet block can generate vibration in response to changes in the magnetic field through its own magnetic properties. It can trigger the shock wave again without waiting for the elastic deformation of the thin film to recover, shortening the response interval of a single sorting and improving the continuous operation efficiency of particle sorting.
[0034] In some embodiments, the length of the conductive blind end 130 is set to 0.2mm to 19mm. In actual design, the length of the conductive blind end 130 can be designed according to actual needs.
[0035] Reference Figure 1In some embodiments, the sorting component 100 further includes a main channel 150, which is connected to the sorting channel 120. The main channel 150 is used to allow non-target particles and target particles to flow and be sorted. Specifically, a particle inlet is provided at the end of the main channel 150 away from the sorting channel 120. Sample liquid is injected into the main channel 150 from the particle inlet using a pneumatic pump or a syringe pump, providing a transport path for non-target particles and target particles to be sorted, ensuring that the particles to be sorted enter the sorting channel 120 in an orderly manner to participate in the sorting operation. At the same time, the main channel 150 and the sorting channel 120 can realize the connection between the transport of particles to be sorted and the sorting operation. In conjunction with the sheath fluid input in the sheath flow channel 110 and the shock wave triggered by the electromagnetic drive component, non-target particles are directed to flow into the first channel 121 and target particles are directed to flow into the second channel 122.
[0036] In some embodiments, the width of the injection cavity 140 is greater than the width of the main channel 150, providing sufficient space for the waves generated by the vibration of the thin film driven by the magnet. This prevents excessive attenuation or diffusion of the waves within the injection cavity 140, ensuring that the waves can cover the cross-sectional area of the main channel 150 during transmission. This guarantees that the shock wave can act on the target particles flowing through the main channel 150, thereby controlling the target particles to flow towards the second channel 122 and improving the response sensitivity and sorting accuracy of the sorting operation. Furthermore, the area of the injection cavity 140 is set to 0.1 mm². 2 ~400mm 2 The width of the main channel 150 is set to 0.02mm to 1mm. Of course, in actual design, the area of the infusion cavity 140 and the width of the main channel 150 can be designed according to actual needs.
[0037] In some embodiments, the width of the main channel 150 is designed so that the main channel 150 can only accommodate one target particle in the vertical direction, thereby avoiding the target particles collected in the main channel 150 from stacking up and down in the vertical direction, so as to facilitate observation.
[0038] Reference Figure 1 In some embodiments, the sheath flow channel 110 includes a first sheath flow channel 111 and a second sheath flow channel 112. Along the axial direction of the main flow channel 150, the first sheath flow channel 111 and the second sheath flow channel 112 are disposed on both sides of the main flow channel 150. By adjusting the sheath flow pressure of the first sheath flow channel 111 and the second sheath flow channel 112, a sheath fluid flow field is formed, which facilitates the control of the flow direction of the particles to be sorted, thereby allowing non-target particles to flow into the first flow channel 121.
[0039] Reference Figure 1In some embodiments, along the axial direction of the main channel 150, the first sheath flow channel 111 and the first flow channel 121 are located on the same side of the main channel 150. The sheath fluid pressure in the first sheath flow channel 111 is less than the sheath fluid pressure in the second sheath flow channel. Combined with the injection pressure of the particles to be sorted, a directional pressure difference flow field can be formed in the main channel 150, thereby causing non-target particles to flow into the first flow channel 121, realizing the directional diversion of non-target particles and ensuring the accuracy of the sorting operation.
[0040] It should be noted that, under the action of the first sheath flow channel 111 and the second sheath flow channel 112, the particles to be sorted flow along the main flow channel 150 in a single ordered state. This part is prior art, and this application has not made any improvements to this part, so its principle and process will not be described in detail.
[0041] Reference Figure 1 In some embodiments, the sorting device further includes a laser detection component (not shown in the figure). The detection end of the laser detection component is located in the main flow channel 150. The laser detection component is used to detect target particles to activate the electromagnetic drive component. Specifically, the detection end of the laser detection component is set as a laser detection line 200. When a target particle passes through the laser detection line 200, the electromagnetic drive component applies a magnetic field of a set frequency. Under the action of the magnetic field, the magnetized body in the injection cavity 140 vibrates with the magnetic field and transmits the shock wave to the target particle through the conduction blind end 130, causing the target particle to flow to the second flow channel 122, thereby achieving the sorting of target particles and non-target particles.
[0042] In some embodiments, after the laser detection line 200 detects the target particle, there is a delay in the sorting device. The particle to be sorted needs to reach the inlet of the sorting channel 120 precisely after this delay. Therefore, the distance between the laser detection line 200 and the inlet of the sorting channel 120 needs to be set according to the delay time of the sorting device and the flow rate of the particle to be sorted. This compensates for the response delay after the sorting device is triggered, ensuring that the target particle reaches the inlet of the sorting channel 120 precisely after the laser detection line 200 detects it and the delay has elapsed. At this point, the shock wave generated by the electromagnetic drive component can act on the target particle, avoiding sorting errors due to the shock wave's position being off-target caused by the delay. This ensures that the target particle can flow into the second channel 122, improving the accuracy and reliability of particle sorting. Furthermore, in actual design, optimizations can be made based on changes in the flow rate of the particle to be sorted under different experimental scenarios and adjustments to the sorting device parameters to meet the sorting requirements of different particles.
[0043] In some embodiments, the sorting device further includes an observation component (not shown in the figure). The inlet end of the sorting channel 120 is located within the working area of the observation component. The flow direction of the particles to be sorted can be observed through the observation component, which facilitates the adjustment of the operating parameters of the sorting device, so that non-target particles flow into the first channel 121 and target particles flow into the second channel 122. Specifically, when non-target particles enter the first channel 121, the injection pressure of the non-target particles entering the main channel 150 and the sheath fluid pressure in the sheath flow channel 110 can be adjusted through real-time feedback from the observation component until it is observed that the non-target particles just flow into the first channel 121 under the combined action of the injection pressure and the sheath fluid pressure, thus completing the adjustment of the injection pressure of the sample solution and the adjustment of the sheath fluid pressure in the sheath flow channel 110. Simultaneously, due to the combined effects of the injection pressure and sheath pressure, non-target particles flow into the first flow channel 121. Even without a shock wave, target particles would also flow into the first flow channel 121. At this point, by adjusting the magnetic force exerted by the electromagnetic drive component on the magnetized body, and observing through the observation component that the target particles, under the influence of the shock wave, flow into the second flow channel 122, the magnetic force adjustment of the electromagnetic drive component on the magnetized body is completed. By using the observation component to assist in adjusting various working parameters, the accuracy of separating non-target and target particles is improved, the probability of mis-sorting during the particle sorting process is reduced, and the reliability and stability of the sorting results are ensured.
[0044] In some embodiments, the observation component is set as a microscope. Of course, in actual design, the structure of the observation component can be designed according to actual needs. It should be noted that observing the flow of particles to be sorted into the first flow channel 121 or the second flow channel 122 through the observation component is prior art, and this application has not made any improvements to this part, so its structure and principle will not be described in detail.
[0045] Reference Figure 2 A second aspect of this application provides a sorting method using the sorting apparatus of the first aspect of this application, comprising the following steps: S100, Processing particles to be sorted; wherein, particles to be sorted include non-target particles and target particles.
[0046] In step S100, the target particles are marked with fluorescent signals, which facilitates the sorting device to identify and sort them.
[0047] S200, inject the sample solution into the main channel 150 of the sorting device.
[0048] In step S200, the sample liquid is injected into the main channel 150 from the particle inlet by a pneumatic pump or a syringe pump, which can provide a transport path for the particles to be sorted and ensure that the particles to be sorted enter the sorting channel 120 in an orderly manner to participate in the sorting operation.
[0049] S300, Adjust the sheath fluid pressure and the injection pressure of the sample fluid in the sheath flow channel 110 so that non-target particles flow into the first flow channel 121.
[0050] In step S300, the sheath fluid pressure in the first sheath flow channel 111 is adjusted to be less than the sheath fluid pressure in the second sheath flow channel 112, and the injection pressure of the sample solution is adjusted. At the same time, the flow direction of non-target particles is observed by the observation component. The injection pressure of the sample solution entering the main flow channel 150 and the sheath fluid pressure in the sheath flow channel 110 are repeatedly adjusted until it is observed that the non-target particles just flow into the first flow channel 121 under the combined action of the injection pressure and the sheath fluid pressure. Then the injection pressure adjustment of the sample solution and the sheath fluid pressure adjustment in the sheath flow channel 110 are completed.
[0051] S400 When the laser detection component of the sorting device detects the target particle, the electromagnetic drive component is activated. The electromagnetic drive component applies a magnetic field to the magnetized body. The magnetized body vibrates with the magnetic field to generate a shock wave. The conduction blind end 130 transmits the shock wave to the target particle to control the target particle to flow into the second flow channel 122.
[0052] In step S400, the position of the laser detection component is adjusted according to the delay time of the sorting device and the flow rate of the particles to be sorted, so that when the electromagnetic drive component is started, the particles to be sorted are located at the inlet end of the sorting channel 120.
[0053] In step S400, the magnetic force exerted by the electromagnetic drive component on the magnetized body is continuously increased until the target particle flows into the second flow channel 122. Specifically, by repeatedly adjusting the magnitude of the magnetic force exerted by the electromagnetic drive component on the magnetized body, and observing that the target particle is just flowing into the second flow channel 122 under the force of the shock wave, the adjustment of the magnetic force exerted by the electromagnetic drive component on the magnetized body is completed.
[0054] In step S400, the start-up time of the electromagnetic drive component is continuously increased so that the shock wave continues to act on the target particles until they flow into the second flow channel 122. Specifically, the start-up time of the electromagnetic drive component is increased from 0 until the target particles are sorted.
[0055] By adjusting the sheath fluid pressure within the sheath flow channel 110, non-target particles are directed into the first flow channel 121. Combined with the detection of target particles by the laser detection component, the electromagnetic drive component is activated. The shock wave generated by the vibration of the magnet is transmitted to the target particle via the conduction blind end 130, controlling the target particle's flow into the second flow channel 122. The entire target particle sorting process eliminates the need for frequent opening and closing of gas valves and the release of high-pressure gas, reducing noise and its interference with the experimental environment, thereby alleviating the auditory burden on operators and improving the comfort of the sorting operation. Simultaneously, the coordinated operation of the sheath fluid pressure regulation within the sheath flow channel 110 and the electromagnetic drive component enables the sorting of non-target and target particles, ensuring the accuracy and reliability of the sorting operation and improving sorting efficiency.
[0056] The embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this application. Furthermore, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other.
Claims
1. A sorting device, characterized in that, include: The sorting assembly includes a sheath flow channel, a sorting channel, and a conductive blind end. The sheath flow channel is connected to the sorting channel and is used to supply sheath fluid flow. The sheath fluid is used to drive the sample fluid flow. The sample fluid contains non-target particles and target particles. The sorting channel includes a first channel and a second channel. The sheath fluid can control the non-target particles to flow into the first channel. The conductive blind end is located at the inlet end of the sorting channel. A perfusion chamber is provided below the conductive blind end, and a magnet is provided in the perfusion chamber. An electromagnetic drive assembly is located directly above the sorting assembly. The electromagnetic drive assembly can apply a magnetic field to the magnetized body, and the magnetized body can vibrate with the magnetic field to generate a shock wave. The conduction blind end can transmit the shock wave to the target particle to control the target particle to flow into the second flow channel.
2. The sorting device according to claim 1, characterized in that, A thin film is disposed between the infusion cavity and the conduction blind end, and the thin film can undergo elastic deformation to generate the shock wave.
3. The sorting device according to claim 1, characterized in that, The sorting component further includes a main channel, which is connected to the sorting channel and is used to allow the sample liquid to flow for sorting.
4. The sorting device according to claim 3, characterized in that, The sheath flow channel includes a first sheath flow channel and a second sheath flow channel, and the first sheath flow channel and the second sheath flow channel are disposed on both sides of the main flow channel along the axial direction of the main flow channel; Along the axial direction of the main flow channel, the first sheath flow channel and the second sheath flow channel are located on the same side of the main flow channel, and the sheath fluid pressure in the first sheath flow channel is less than the sheath fluid pressure in the second sheath flow channel.
5. The sorting device according to claim 3, characterized in that, The sorting device further includes a laser detection component, the detection end of which is located in the main channel. The laser detection component is used to detect the target particles in order to activate the electromagnetic drive component.
6. The sorting device according to claim 1, characterized in that, The magnetized body is configured as a magnetic fluid, a magnetized metal powder, or a magnet block.
7. A sorting method based on the sorting device according to any one of claims 1 to 6, characterized in that, Includes the following steps: Processing particles to be sorted; wherein, the particles to be sorted include the non-target particles and the target particles; The sample solution is injected into the main channel of the sorting device; Adjust the sheath fluid pressure and the sample fluid injection pressure within the sheath flow channel to allow the non-target particles to flow into the first flow channel; When the laser detection component of the sorting device detects the target particle, the electromagnetic drive component is activated. The electromagnetic drive component applies a magnetic field to the magnetized body, and the magnetized body vibrates with the magnetic field to generate a shock wave. The conduction blind end transmits the shock wave to the target particle to control the target particle to flow into the second flow channel.
8. The sorting method according to claim 7, characterized in that, The electromagnetic drive assembly applies a magnetic field to the magnetized body, including: The magnetic force exerted by the electromagnetic drive component on the magnetized body is continuously increased until the target particle flows into the second flow channel.
9. The sorting method according to claim 7, characterized in that, The electromagnetic drive assembly applies a magnetic field to the magnetized body, including: The startup time of the electromagnetic drive component is continuously increased so that the shock wave continues to act on the target particle until it flows into the second flow channel.
10. The sorting method according to claim 7, characterized in that, The laser detection component of the sorting device detects the target particles, including: The position of the laser detection component is adjusted according to the delay time of the sorting device and the flow rate of the particles to be sorted, so that when the electromagnetic drive component is started, the particles to be sorted are located at the inlet end of the sorting channel.