Bottle adapter, system and method

Abstract

A sample vial adapter for use in liquid chromatography, the sample vial adapter comprising a body structure extending between a top end and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body such that the channel at least partially surrounds the received sample vial, and a body structure including a channel opening configured to receive the sample vial, the body structure including an extension that reduces a width of the channel opening, at least one of the extension and the body structure being configured to elastically deform during receiving the sample vial.

Description

Related Applications

[0001] This application claims priority to U.S. Provisional Patent Application No. 63 / 535,718, filed August 31, 2024, and entitled “Vial Adapter, System and Method,” the entirety of which is incorporated herein by reference. TECHNICAL FIELD

[0002] The present invention relates generally to liquid chromatography systems. More specifically, the present invention relates to liquid chromatography sample managers and associated systems and methods. BACKGROUND

[0003] Chromatography is a group of techniques for separating mixtures into their components. For example, in a liquid chromatography system, a pump draws in a mixture of liquid solvents and delivers it to a sample manager, where an injected sample awaits its arrival. In isocratic chromatography systems, the composition of the liquid solvents remains constant, while in gradient chromatography systems, the solvent composition varies over time. A mobile phase consisting of a sample dissolved in the solvent mixture passes through a column, known as a stationary phase. By passing the mixture through the column, the various components in the sample separate from one another at different rates and thus elute from the column at different times. A detector receives the elution from the column and produces an output from which the identity and quantity of the analytes can be determined.

[0004] Before a sample is provided into a liquid chromatography system, it can be provided to a sample manager. The sample manager can be configured to prevent the sample from degrading or becoming compromised while it is provided into the liquid chromatography system. The sample manager typically interacts with a technician, and therefore must be user-friendly, reliable, accurate, robust, durable, and cost-effective.

[0005] Some sample managers can receive a number of sample vials that can be disposed within a sample vial tray or rack. Other sample managers require a technician to input individual vials. Such single-input sample managers can deploy, for example, a carousel system, whereby the technician is provided access to a single location on the carousel to insert a new single vial. Due to the complexity of the carousel and needle system within the sample manager, such sample managers are typically able to accept vials of a single size and / or dimensional arrangement. SUMMARY

[0006] In one embodiment, a liquid chromatography system includes a solvent delivery system; a sample manager having a heat treatment chamber including a sample delivery system in fluid communication with the solvent delivery system, the sample delivery system including a sample needle, the sample delivery system configured to transfer a sample from a sample vial located in the heat treatment chamber into a chromatography flow stream; a sample vial adapter having a body structure extending between a top end and a bottom end and defining a channel for receiving the sample vial having a cylindrical sample vial body such that the channel at least partially encircles the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion that reduces a width of the channel opening, at least one of the protrusion and the body structure configured to elastically deform during receipt of the sample vial; a liquid chromatography column located downstream of the solvent delivery system and the sample delivery system; and a detector located downstream of the liquid chromatography column.

[0007] Additionally or alternatively, the body structure is composed of a plastic material.

[0008] Additionally or alternatively, the top end of the body structure includes a mock cap structure, wherein the mock cap structure is sized to resemble a cap, the mock cap structure is an integral feature of the body structure, and / or the mock cap structure is not removable from the body structure. Further, the mock cap structure can include a top surface having an opening that provides access for the needle into the received sample vial.

[0009] Additionally or alternatively, the body structure further includes a back opening located at the bottom of the channel, the back opening configured to provide access for a technician's finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel. Further, the body structure can include a second back opening configured to provide access for a second technician's finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel.

[0010] Additionally or alternatively, the body structure further includes a second protrusion located at the same axial position as the protrusion, the second protrusion further reducing the width of the channel opening, the protrusion and the second protrusion forming a pair of protrusions configured to retain the received sample vial within the channel. Further, the sample vial adapter can further include a second pair of protrusions located at a different axial position than the pair of protrusions.

[0011] Additionally or alternatively, the body structure forms a substantially cylindrical shape having a removed channel opening.

[0012] Additionally or alternatively, at least one of the body structure and the extension is configured to return to an original position after receiving a sample vial and to retain the received sample vial within the channel.

[0013] In another embodiment, a liquid chromatography sample manager includes a heat treatment chamber, a sample delivery system including a sample needle, the sample delivery system configured to transfer a first sample from a sample vial located in the heat treatment chamber into a chromatography flow stream, and a sample vial adapter having a body structure extending between a top end and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body such that the channel at least partially encircles the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including an extension that reduces a width of the channel opening, at least one of the extension and the body structure is configured to elastically deform during receipt of the sample vial, at least one of the body structure and the extension is configured to return to an original position after receiving a sample vial and to retain the received sample vial within the channel.

[0014] Additionally or alternatively, the body structure is composed of a plastic material.

[0015] Additionally or alternatively, the top end of the body structure includes a mock cap structure, wherein the mock cap structure is sized to resemble a cap, the mock cap structure is an integral feature of the body structure, and / or the mock cap structure is not removable from the body structure. Further, the mock cap structure can include a top surface having an opening that provides access for the needle into the received sample vial.

[0016] Additionally or alternatively, the body structure further includes a back opening at a bottom of the channel configured to provide access for a technician’s finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel. Further, the body structure can include a second back opening configured to provide access for a second technician’s finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel.

[0017] Additionally or alternatively, the body structure further includes a second extension at a same axial position as the extension, the second extension further reducing the width of the channel opening, the extension and the second extension forming a pair of extensions configured to retain the received sample vial within the channel. Further, the sample vial adapter can further include a second pair of extensions at a different axial position than the pair of extensions.

[0018] Additionally or alternatively, the body structure forms a substantially cylindrical shape having a removed channel opening.

[0019] Additionally or alternatively, at least one of the body structure and the protrusion is configured to return to an original position after receiving the sample bottle and retain the received sample bottle within the channel.

[0020] In another embodiment, a sample bottle adapter for use in liquid chromatography includes a body structure extending between a top end and a bottom end and defining a channel for receiving a sample bottle having a cylindrical sample bottle body such that the channel at least partially encircles the received sample bottle, the body structure including a channel opening configured to receive the sample bottle, the body structure including a protrusion reducing a width of the channel opening, at least one of the protrusion and the body structure being configured to elastically deform during receipt of the sample bottle.

[0021] Additionally or alternatively, the body structure is composed of a plastic material.

[0022] Additionally or alternatively, the top end of the body structure includes a mock cap structure, wherein the mock cap structure is sized to resemble a cap, the mock cap structure is an integral feature of the body structure, and / or the mock cap structure is not removable from the body structure. Further, the mock cap structure can include a top surface having an opening providing access for a needle into the received sample bottle.

[0023] Additionally or alternatively, the body structure further includes a back opening at a bottom of the channel configured to provide access for a technician’s finger to press the received sample bottle through the body structure to facilitate removal of the sample bottle from the channel. Further, the body structure can include a second back opening configured to provide access for a second technician’s finger to press the received sample bottle through the body structure to facilitate removal of the sample bottle from the channel.

[0024] Additionally or alternatively, the body structure further includes a second protrusion at a same axial position as the protrusion, the second protrusion further reducing the width of the channel opening, the protrusion and the second protrusion forming a pair of protrusions configured to retain the received sample bottle within the channel. Further, the sample bottle adapter can further include a second pair of protrusions at a different axial position than the pair of protrusions.

[0025] Additionally or alternatively, the body structure forms a substantially cylindrical shape having a removed channel opening.

[0026] Additionally or alternatively, at least one of the body structure and the protrusion is configured to return to an original position after receiving the sample bottle and retain the received sample bottle within the channel.

[0027] In another embodiment, a chromatographic method includes providing a liquid chromatography system including: a solvent delivery system; a sample manager having a heat treatment chamber including a sample delivery system in fluid communication with the solvent delivery system, the sample delivery system including a sample needle, the sample delivery system configured to transfer a sample from a sample vial located in the heat treatment chamber to a chromatographic flow stream; a sample vial adapter having a body structure extending between a top end and a bottom end and defining a channel for receiving the sample vial having a cylindrical sample vial body such that the channel at least partially encircles the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion that reduces a width of the channel opening, at least one of the protrusion and the body structure configured to elastically deform during receipt of the sample vial; a liquid chromatography column located downstream of the solvent delivery system and the sample delivery system; and a detector located downstream of the liquid chromatography column. The method further includes: providing the sample vial; inserting the sample vial into the sample vial adapter by elastically deforming the at least one of the protrusion and the body structure; inserting the sample vial adapter with the sample vial into the heat treatment chamber of the sample manager; interacting with the sample vial with the sample needle through the sample vial adapter; injecting the sample into the chromatographic flow stream with the sample needle; separating the sample with the liquid chromatography column; and detecting the sample with the detector.

[0028] Additionally or alternatively, the chromatographic method can further include: removing the sample vial adapter and the sample vial from the heat treatment chamber of the sample manager; removing the sample vial from the sample vial adapter; inserting a second sample vial into the sample vial adapter; inserting the sample vial adapter with the second sample vial into the heat treatment chamber of the sample manager; interacting with the second sample vial with the sample needle through the sample vial adapter; injecting the second sample into the chromatographic flow stream with the sample needle; separating the second sample with the liquid chromatography column and / or detecting the second sample with the detector.

[0029] In yet another embodiment, a method of adapting a sample vial includes determining a circumference of a sample vial, wherein the sample vial is substantially cylindrical; determining a height of the sample vial; determining a size of an acceptable sample vial, wherein the acceptable sample vial is substantially cylindrical and has a circumference and a height that are greater than the circumference and the height of the sample vial, wherein the acceptable sample vial is configured to be individually received and processed by an automated sample manager; and forming a sample vial adapter having a body structure extending between a top end and a bottom end and defining a channel for receiving the sample vial such that the channel at least partially encircles a received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion that reduces a width of the channel opening, at least one of the protrusion and the body structure being configured to elastically deform during receipt of the sample vial, at least one of the body structure and the protrusion being configured to return to an original position after receipt of the sample vial and retain the received sample vial within the channel. BRIEF DESCRIPTION OF DRAWINGS

[0030] The above advantages and other advantages of the present application are better understood when considered in connection with the following description and the accompanying drawings, in which like reference characters designate the same elements or features in the various figures, wherein:

[0031] Figure 1 A schematic diagram of a liquid chromatography system including a sample manager is depicted in accordance with one embodiment.

[0032] Figure 2 A perspective view of a liquid chromatography system including a sample manager in accordance with one embodiment is depicted. Figure 1

[0033] Figure 3 A sample vial is depicted in accordance with one embodiment.

[0034] Figure 4 A perspective view of a sample vial adapter in accordance with one embodiment is depicted.

[0035] Figure 5 A perspective view of a sample vial adapter in accordance with one embodiment is depicted. Figure 3 Figure 4

[0036] Figure 6 A perspective view of a sample vial adapter in accordance with one embodiment is depicted. Figure 4 Figure 3

[0037] ​​​​​Figure 7 A method of adapting a sample vial according to one embodiment is depicted.

[0038] Figure 8 A chromatography method according to one embodiment is depicted.

[0039] Figure 9 A further chromatography method according to one embodiment is depicted. DETAILED DESCRIPTION

[0040] Reference to“one embodiment” or“an embodiment” within this specification

[0041] The present teachings will now be described in greater detail in connection with the exemplary embodiments of the present teachings as illustrated in the following drawings. While the present teachings are described in conjunction with various embodiments and examples, it should be understood that the present teachings are not limited to such embodiments. Rather, the present teachings cover various alternatives, modifications and equivalents, as would be appreciated by one of ordinary skill in the art. Additional embodiments, modifications, and embodiments within the scope of the present teachings as described herein will be recognized by those of ordinary skill in the art. It will be recognized that additional embodiments, modifications, and embodiments within the scope of the present teachings will become apparent and / or recognized from consideration of the specification and / or drawings and / or practice of the teachings as set forth herein.

[0042] As described herein, prior to conducting a liquid chromatography run, a technician loads a vial containing a sample into a sample manager vessel or receptacle of individual sample vials. The sample manager system includes a sample delivery system configured to transfer the sample from the sample vial holder to the chromatography flow stream. The heat treatment chamber includes a sampling mechanism including a rotating sample vial turntable in order to process a sample that has been provided to the vessel or receptacle. At least one sampling needle that is part of the sampling mechanism is located within and / or accessible to the heat treatment chamber that can draw a sample from the vial and inject the sample into the chromatography flow stream. As contemplated herein, the sample manager and sample delivery system along with its turntable can be particularly configured for sample vials having acceptable, preferred, and / or specifically defined dimensions. Due to the engineering complexity of the turntable, sensor, and needle design, such a system can not be configured for sample vials having dimensions different than the acceptable, preferred, and / or specifically defined dimensions. However, it can be desirable to process sample vials having dimensions different than the acceptable, preferred, and / or specifically defined dimensions by such a configured sample manager. In such instances, the present embodiments contemplate changing the dimensions of different sample vials with a sample vial adapter in order to be able to be processed by the sample manager that would not otherwise be configured to process the different sample vial due to the different dimensions. While the embodiments described herein can be particularly applicable to single load sample vial systems having a turntable system, the application of a sample vial adapter as described herein can be applicable to any system where it is desirable to change the dimensions of a sample vial for the purpose of facilitating the automated system's interaction with the sample vial.

[0043] The features of the sample delivery system and sample manager heat treatment chamber described herein can be applicable to any liquid chromatography system configured to deliver a sample to a chromatography flow stream. As one example, Figure 1 An embodiment of a liquid chromatography system 10 for separating a mixture into its components is shown. The liquid chromatography system 10 includes a solvent delivery system 12 in fluid communication with a sample manager 14 (also referred to as a sampler or autosampler) through tubing 16. The sample manager 14 is in fluid communication with a chromatography column 18. A detector 21, such as a mass spectrometer, is in fluid communication with the column 18 to receive elutions.

[0044] The solvent delivery system 12 includes a pumping system 20 in fluid communication with a solvent reservoir 22 from which the pumping system 20 draws solvent (liquid) through tubing 24. In one embodiment, the pumping system 20 is embodied by a hybrid system having one or more pumps. The liquid chromatography system 10 can be, for example, an ultra performance liquid chromatography (UPLC) system, although the present application is not limited to this embodiment. For example, in a low pressure gradient pumping system, mixing of solvents occurs prior to the pump, and the solvent delivery system 12 has a mixer 26 in fluid communication with the solvent reservoir 22 to receive various solvents in metered proportions. This mixing of the solvent (mobile phase) composition is varied over time (i.e., varies in a gradient). However, in other embodiments, mixing can occur after the pumping system 20 but before providing the chromatographic stream to the sample manager 14.

[0045] The pumping system 20 is shown in fluid communication with the mixer 26 to draw a continuous gradient stream therefrom for delivery to the sample manager 14. Examples of solvent delivery systems that can be used to implement the solvent delivery system 12 include, but are not limited to, the ACQUITY Binary Solvent Manager and the ACQUITY Quaternary Solvent Manager manufactured by Waters Corp., Milford, Mass.

[0046] The sample manager 14 can include an injector valve 28 having a sample loop 30. The sample manager 14 can operate in at least two states: a load state and an injection state. In the load state, the position of the injector valve 28 is such that the sample manager loads a sample 32 into the sample loop 30. The sample 32 is drawn from a vial contained in a sample vial holder. A "sample vial holder" herein refers to any device configured to hold a sample vial, such as a well plate, a sample vial holder, etc. In the injection state, the position of the injector valve 28 is changed such that the sample manager 14 introduces the sample in the sample loop 30 from the solvent delivery system into the continuous flow mobile phase. Thus, the mobile phase carries the sample into the column 18. In other embodiments, a flow through needle (FTN) can be utilized instead of a fixed loop sample manager. Using the FTN method, a sample can be pulled into a needle, and then the needle can be moved into a septum. A valve can then be switched to put the needle in line with the solvent delivery system.

[0047] The liquid chromatography system 10 also includes a data system 34 in signal communication with the solvent delivery system 12 and the sample manager 14. The data system 34 has a processor 36 and a switch 38 (e.g., an Ethernet switch) for handling signal communication between the solvent delivery system 12 and the sample manager 14. Signal communication between the various systems and instruments can be electrical or optical, using wireless or wired transmission. A host computing system 40 is in communication with the data system 34 through which a technician can download various parameters and configuration files (e.g., an inlet gas velocity configuration file) to the data system 34.

[0048] Figure 2 A perspective view of the liquid chromatography system 10 is shown, including the sample manager 14, the detector 21, the chromatography column 18, the solvent delivery system 12, and the pump system 20, as well as the solvent 22. Each of the sample manager 14, the detector 21, the chromatography column 18, the solvent delivery system 12 can include a housing or body within which various features can be housed. Further, the entire liquid chromatography system 10 is connected to a data system 34 (e.g., a computer system) that includes a user interface and / or display device 41. The various components 12, 14, 18, 19, 21, 22 of the liquid chromatography system 10 can be interconnected with fluidic tubing and in signal communication with the data system 34 of the system and / or the host computing system 40. The liquid chromatography system 10 is shown with the solvent delivery system 12, the sample manager 14, the chromatography column 18, the detector 21, and a plurality of trays for holding solvent 22, as well as a waste container 42. The liquid chromatography system 10 can be, for example, a PATROL UPLC system manufactured by Waters Corp., Milford, Mass.

[0049] Figure 3 A sample vial 100 is depicted in accordance with one embodiment. The sample vial 100 includes a body 110 extending between a top end 112 and a bottom end 114. Near the top end 114, the sample vial 100 also includes a top cap 116. The top cap 116 can be threadedly engaged or crimped to the body 110 of the sample vial 100. In other embodiments, the top cap 116 can be attached to the body 110, for example, by a press fit or an interference fit. The top cap 116 can include a top structure 115 that can be configured to hold a sample within the sample vial 100, but can also be thin enough to be pierced by a sample needle (not shown) from the sample manager 14.

[0050] The body 110 of the sample bottle 100 can be generally cylindrical in shape. The top end 114 of the generally cylindrical body 110 can include a narrowed or tapered portion 117. A neck 118 extends from the narrowed or tapered portion 117. The neck 118 can include external threading for receiving internal threading of the top cap 116 in a threaded arrangement.

[0051] The dimensions of the sample bottle 100 shown are exemplary, and the principles of the embodiments described herein can be applicable to any sample bottle structure having a generally cylindrical or cylindrical body. In other embodiments, various dimensions of the sample bottle can be different, such as the height of the sample bottle, the circumference of the body, the shape of the top end 112 (i.e., the shape or taper of the narrowed portion 117 and the dimensions of the neck 118), the height or circumference of the top cap 116, etc. In some embodiments, the sample bottle can not include a tapered portion or neck, and can simply include a cylindrical body extending to a threaded end for receiving a top cap. Regardless of the embodiment, the present disclosure contemplates shaping the sample bottle adapter to match and / or correspond to the dimensions of the sample bottle 100, as described below.

[0052] Figure 4 A perspective view of a sample bottle adapter 200 according to one embodiment is depicted. The sample bottle adapter 200 includes a body structure 210 extending between a top end 212 and a bottom end 214. The body structure 210 defines a channel 216 for receiving a sample bottle 100. While a particular channel dimension of the channel 216 has been shown to fit the dimensions of the sample bottle 100, other channel dimensions can be contemplated in order to accommodate sample bottles of different sizes and dimensions.

[0053] In any case, the channel 216 can be sized to at least partially encircle the received sample bottle 100. The channel 216 can extend a majority of the length of the body structure 210 from the top end 212 to the bottom end 214. The channel 216 can wrap about 180 degrees around the sample bottle 100. In some embodiments, the channel 216 can wrap greater than or less than 180 degrees around the sample bottle 100.

[0054] The body structure 210 includes a channel opening 218 configured to receive the sample bottle 100 within the channel 216. The body structure 210 also includes a plurality of extensions 220a, 220b, 220c, 220d that reduce the width of the channel opening 218. The extensions 220a, 220b, 220c, 220d are shown to include curved extending edges 222 that extend the extensions 220a, 220b, 220c, 200d into the channel opening 218. The extensions 220a, 220b, 220c, 220d maintain the curvature of the circumference of the channel 216 and the body structure 210 and extend to a flat axially extending edge 224. In other embodiments, the extensions 220a, 220b, 220c, 220d can be slightly curved inwardly such that the received bottle will be held in the channel 216 by a constant biasing force provided by the extensions 220a, 220b, 220c, 220d.

[0055] Regardless of the embodiment, the first extension 220a and the second extension 220b can form a first pair of extensions located at a first axial position of the body structure 210, while the third extension 220c and the fourth extension 220d can form a second pair of extensions located at a second axial position of the body structure that is lower than the first axial position. Any number of pairs of extensions can be envisioned. For example, the body structure 210 can include a single pair of extensions, or can include more than two pairs of extensions. In some cases, the body structure 210 can not use pairs of extensions, but can include a single flexible extension that extends from only a single side of the channel opening 218.

[0056] The extensions 220a, 220b, 220c, 220d and / or the body structure 210 can each be configured to elastically deform and / or deflect during the process of receiving the sample bottle 100. This process is shown more clearly in Figures 5 to 6 The extensions 220a, 220b, 220c, 220d and / or the body structure 210 can be configured to return to the original position (i.e., as shown in Figure 4 FIG. 1) upon receiving the sample bottle 100 and to hold the received sample bottle 100 within the channel 216. To achieve this deflection or elastic deformation, the body structure of the sample bottle adapter 200 can be made of, for example, a plastic material, a polymer, a composite material, or even a flexible metal material. Regardless of the material, the flexibility should be such that the extensions 220a, 220b, 220c, 220d can flex when receiving the sample bottle 100 and allow the sample bottle 100 to snap into place within the channel 216 via, for example, a snap fit, after which the sample bottle 100 is securely held in the channel 216 until intentionally removed. Removal can require the extensions to flex or elastically deform again in order to allow the sample bottle 100 to be removed.

[0057] In some embodiments, it is contemplated that a sample vial received in the channel 216 via a snap fit can be held against a small degree of outward flexing force on the protrusions 220a, 220b, 220c, 220d. For example, when the sample vial 100 is received in the channel 216, the circumference or size of the sample vial 100 can be large enough to extend into the space occupied by the original position of the protrusions 220a, 220b, 220c, 220d.

[0058] The top end 212 of the body structure 210 includes a mock cap structure 230. The mock cap structure 230 can be designed in a size similar to an actual cap. However, the mock cap structure 230 can instead be an integral feature of the body structure 210 and can not be removable from the body structure 210. The mock cap structure 230 includes a top surface 232 having an opening 234. The opening 234 can be configured to provide access for a sample needle or syringe needle into a received sample vial 100.

[0059] The body structure 210 also includes a back opening 240 at the bottom of the channel 216. The back opening 240 can be configured to provide access for a technician’s finger to press a received sample vial 100 through the body structure 210 to facilitate removal of the sample vial 210 from the channel 216 and the body structure 210. While a single opening is contemplated as shown in the figures, in other embodiments, more than one back opening can be included. For example, the body structure 210 can include a second back opening configured to provide access for a second finger of a technician to press a received sample vial 100 through the body structure 210 to facilitate removal of the sample vial 100 from the channel 216 and the body structure 210.

[0060] The body structure 210 forms a substantially cylindrical shape with the removed channel opening 216. This shape can be configured to mimic, represent, or replicate an acceptable, preferred, and / or standard shape of a sample vial configured for a particular sample management system. The acceptable, preferred, and / or standard shape of a sample vial can be a shape that is specifically designed to be acceptable to a sample vial holder system, needle design, and sensor system of a sample manager that has been specifically configured to be optimized for that acceptable or preferred shape and size. While the contemplated embodiment shows one example of an acceptable or preferred shape, the body structure 210 can take other shapes depending on the design of the corresponding sample manager system.

[0061] Figure 5 A sample vial 100 partially received in a channel 216 of a body structure 210 is depicted in accordance with one embodiment Figure 3 Figure 4 ​A perspective view of a sample vial adapter 200. As shown, the bottom of a sample vial 100 is shown first inserted into the sample vial adapter 200. Once this occurs, the top of the sample vial 100 can be pressed into the sample vial adapter 200, deflecting the body structure 210 and / or the protrusions 220a, 220b, 220c, 220d of the sample vial adapter 200 in order to snap the sample vial 100 into place within the channel 216. Figure 6 A perspective view of a sample vial adapter 200 according to one embodiment is depicted. Figure 4 A perspective view of a sample vial adapter 200 according to one embodiment is depicted. Figure 3 A perspective view of a sample vial adapter 200 according to one embodiment is depicted.

[0062] Figure 7 A method 700 of adapting a sample vial, such as sample vial 100, according to one embodiment is depicted. The method 700 can include a first step 710 of determining a circumference of a sample vial, such as sample vial 100. The sample vial 100 can be substantially cylindrical. The method 700 can include a second step 720 of determining a height of the sample vial.

[0063] The method 700 can then include a step 730 of determining acceptable dimensions of a sample vial for a given sample manager. These dimensions can be well known and can represent dimensions of a standard sample vial that is configured for use with a given automated sample manager and can also be configured to be individually received and processed by the sample manager. The acceptable sample vial can also be substantially cylindrical in shape and can have a circumference and a height that are greater than the circumference and height of the sample vial of steps 710, 720.

[0064] The method 700 then includes a step of forming a sample vial adapter, such as sample vial adapter 200. The sample vial adapter can be formed to include a body structure, such as body structure 210, that extends between a top end and a bottom end and defines a channel, such as channel 216, for receiving a sample vial such that the channel at least partially encircles the received sample vial. The body structure 210 can also be formed to include a channel opening 218 configured to receive the sample vial. The body structure can also be formed to include a protrusion that reduces a width of the channel opening, wherein either or both of the protrusion and the body structure are configured to elastically deform during a process of receiving the sample vial and subsequently return to an original position and retain the received sample vial within the channel after the sample vial is received.

[0065] The contemplated method also includes utilizing the sample vial adapter in a chromatographic method, such as within the liquid chromatography system 10, in order to separate and detect a sample. Figure 8A chromatography method 800 according to one embodiment is depicted. The chromatography method 800 includes a step 810 of providing a liquid chromatography system, such as the liquid chromatography system 10. The method 800 includes a step 820 of providing a sample vial, such as the sample vial 100. The method 800 includes a step 830 of inserting the sample vial into a sample vial adapter, such as the sample vial adapter 200, by elastically deforming the body structure of the sample vial adapter. Then, the method 800 includes a step 840 of inserting the sample vial adapter with the sample vial into a heat treatment chamber of a sample manager. The method 800 includes a step 850 of interacting with the sample vial with a sample needle by the sample vial adapter, and a step 860 of injecting a sample into a chromatography flow stream with the sample needle. Then, the method 800 includes a step 870 of separating the sample with a liquid chromatography column, such as the column 18, and a step 880 of detecting the sample with a detector, such as the detector 21.

[0066] Figure 9 A further chromatography method 900 according to one embodiment is depicted. The method 900 can be a continuation of the method 800 described above. The method 900 includes a first step 910 of removing the sample vial adapter and the sample vial from the heat treatment chamber of the sample manager. Then, the method 900 includes a step 920 of removing the sample vial from the sample vial adapter. Then, the method 900 includes a step 930 of inserting a second sample vial into the sample vial adapter. The second sample vial can have the same dimensions as the first sample vial, but can include its own different sample therein. Then, the method 900 can include a step 940 of inserting the sample vial adapter with the second sample vial into the heat treatment chamber of the sample manager, and a step 950 of interacting with the second sample vial with a sample needle by the sample vial adapter. Then, the method 900 can include a step 960 of injecting a second sample into the chromatography flow stream with the sample needle. Finally, the method 900 can include a step 970 of separating the second sample with the liquid chromatography column, and a step 980 of detecting the second sample with the detector.

[0067] While the application has been shown and described with reference to particular embodiments, it will be apparent to those skilled in the art that various changes in form and detail can be made without departing from the spirit and scope of the application as set forth in the following claims.

Claims

1. A liquid chromatography system, the liquid chromatography system comprising: Solvent delivery system; A sample manager having a heat treatment chamber including a sample delivery system in fluid communication with a solvent delivery system, the sample delivery system including a sample needle, the sample delivery system being configured to transfer a sample from a sample vial located in the heat treatment chamber into a chromatographic flow stream; A sample vial adapter having a body structure extending between a top and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body, such that the channel at least partially surrounds the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion reducing the width of the channel opening, and at least one of the protrusion and the body structure being configured to elastically deform during the reception of the sample vial; A liquid chromatography column, wherein the liquid chromatography column is located downstream of the solvent delivery system and the sample delivery system; and A detector located downstream of the liquid chromatography column.

2. The liquid chromatography system according to claim 1, wherein the main structure is composed of a plastic material.

3. The liquid chromatography system according to claim 1, wherein the top end of the main body structure includes a replica top cover structure, wherein the dimensions of the replica top cover structure are designed to resemble a top cover, wherein the replica top cover structure is an integral feature of the main body structure, and wherein the replica top cover structure cannot be removed from the main body structure.

4. The liquid chromatography system of claim 3, wherein the replica top cap structure includes a top surface having an opening, wherein the opening provides a pathway for a needle to enter the received sample vial.

5. The liquid chromatography system of claim 1, wherein the body structure further includes a back opening located at the bottom of the channel, the back opening being configured to provide a passage for a technician's fingers to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel.

6. The liquid chromatography system of claim 5, wherein the body structure includes a second back opening configured to provide a passage for the technician's second finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the passage.

7. The liquid chromatography system of claim 1, wherein the main structure further includes a second extension located at the same axial position as the extension, the second extension further reducing the width of the channel opening, the extension and the second extension forming a pair of extensions configured to retain a received sample vial within the channel.

8. The liquid chromatography system according to claim 7, wherein the liquid chromatography system further comprises a second pair of protrusions located at an axial position different from the pair of protrusions.

9. The liquid chromatography system of claim 1, wherein the main structure is formed in a substantially cylindrical shape with removed channel openings.

10. The liquid chromatography system of claim 1, wherein at least one of the main structure and the extension is configured to return to its original position after receiving the sample vial and to retain the received sample vial within the channel.

11. A liquid chromatography sample manager, the liquid chromatography sample manager comprising: Heat treatment chamber; A sample delivery system, the sample delivery system including a sample needle, the sample delivery system being configured to transfer a first sample from a sample vial located in the heat treatment chamber to a chromatographic flow stream; and A sample vial adapter having a body structure extending between a top and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body, such that the channel at least partially surrounds the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion reducing the width of the channel opening, and at least one of the protrusion and the body structure being configured to elastically deform during the reception of the sample vial.

12. The liquid chromatography sample manager according to claim 11, wherein the main structure is composed of a plastic material.

13. The liquid chromatography sample manager of claim 11, wherein the top of the main body structure includes a replica top cover structure, wherein the dimensions of the replica top cover structure are designed to resemble a top cover, wherein the replica top cover structure is an integral feature of the main body structure, and wherein the replica top cover structure cannot be removed from the main body structure.

14. The liquid chromatography sample manager of claim 13, wherein the replica top cap structure includes a top surface having an opening, wherein the opening provides a pathway for a needle to enter the received sample vial.

15. The liquid chromatography sample manager of claim 11, wherein the body structure further includes a back opening at the bottom of the channel, the back opening being configured to provide passage for a technician's fingers to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel.

16. The liquid chromatography sample manager of claim 15, wherein the body structure includes a second back opening configured to provide a passage for the technician's second finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the passage.

17. The liquid chromatography sample manager of claim 11, wherein the main body structure further includes a second extension located at the same axial position as the extension, the second extension further reducing the width of the channel opening, the extension and the second extension forming a pair of extensions configured to retain the received sample vial within the channel.

18. The liquid chromatography sample manager according to claim 17, further comprising a second pair of protrusions located at an axial position different from the pair of protrusions.

19. The liquid chromatography sample manager of claim 11, wherein the main body structure is formed in a substantially cylindrical shape with removed channel openings.

20. The liquid chromatography sample manager of claim 11, wherein at least one of the main body structure and the extension is configured to return to its original position after receiving the sample vial and to retain the received sample vial within the channel.

21. A sample vial adapter for use in liquid chromatography, the sample vial adapter comprising: A main structure extending between a top and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body, such that the channel at least partially surrounds the received sample vial, the main structure including a channel opening configured to receive the sample vial, the main structure including a protrusion reducing the width of the channel opening, and at least one of the protrusion and the main structure being configured to elastically deform during the reception of the sample vial.

22. The sample vial adapter according to claim 21, wherein the main structure is made of a plastic material.

23. The sample bottle adapter of claim 21, wherein the top end of the main body structure includes a replica top cap structure, wherein the dimensions of the replica top cap structure are designed to resemble a top cap, wherein the replica top cap structure is an integral feature of the main body structure, and wherein the replica top cap structure cannot be removed from the main body structure.

24. The sample vial adapter of claim 23, wherein the replica top cap structure includes a top surface having an opening, wherein the opening provides passage for a needle to enter the received sample vial.

25. The sample vial adapter of claim 1, wherein the body structure further includes a back opening located at the bottom of the channel, the back opening being configured to provide passage for a technician's fingers to press the received sample vial through the body structure to facilitate removal of the sample vial from the channel.

26. The sample vial adapter of claim 25, wherein the body structure includes a second back opening configured to provide a passage for the technician's second finger to press the received sample vial through the body structure to facilitate removal of the sample vial from the passage.

27. The sample vial adapter of claim 26, wherein the body structure further includes a second extension located at the same axial position as the extension, the second extension further reducing the width of the channel opening, the extension and the second extension forming a pair of extensions configured to retain the received sample vial within the channel.

28. The sample vial adapter of claim 27, further comprising a second pair of protrusions located at an axial position different from the pair of protrusions.

29. The sample vial adapter of claim 27, wherein the body structure is formed in a substantially cylindrical shape with a removed channel opening.

30. The sample vial adapter of claim 21, wherein at least one of the main body structure and the extension is configured to return to its original position after receiving the sample vial and to retain the received sample vial within the channel.

31. A chromatographic method, the chromatographic method comprising: Provide a liquid chromatography system according to claim 1; Provide the sample bottle; The sample vial is inserted into the sample vial adapter by elastically deforming at least one of the protrusion and the main body structure; Insert the sample vial adapter and the sample vial together into the heat treatment chamber of the sample manager; The sample needle interacts with the sample vial via the sample vial adapter; The sample is injected into the chromatographic flow using the sample needle; The sample was separated using the liquid chromatography column; as well as The sample is detected using the detector.

32. The chromatographic method according to claim 31, further comprising: Remove the sample vial adapter and the sample vial from the heat treatment chamber of the sample manager; Remove the sample bottle from the sample bottle adapter; Insert the second sample vial into the sample vial adapter; Insert the sample vial adapter together with the second sample vial into the heat treatment chamber of the sample manager; The sample needle interacts with the second sample vial via the sample vial adapter; The second sample is injected into the chromatographic flow using the sample needle; The second sample was separated using the liquid chromatography column; as well as The second sample is detected using the detector.

33. A method for adapting a sample vial, the method comprising: Determine the circumference of the sample vial, wherein the sample vial is substantially cylindrical; Determine the height of the sample bottle; Determine the dimensions of an acceptable sample vial, wherein the acceptable sample vial is substantially cylindrical and has a circumference and height greater than the circumference and height of the sample vial, wherein the acceptable sample vial is configured to be individually received and processed by an automated sample manager; as well as A sample vial adapter is formed, the sample vial adapter including a body structure extending between a top and a bottom end and defining a channel for receiving the sample vial such that the channel at least partially surrounds the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including a protrusion reducing the width of the channel opening, at least one of the protrusion and the body structure being configured to elastically deform during the reception of the sample vial.

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