Culture medium convolution oscillator

Abstract

The invention provides a culture medium convolution oscillator which comprises an oscillator main machine, a reagent frame assembly, a self-locking unit and a wedge-shaped frame, the reagent frame assembly comprises a bottom frame, a lower-layer grating, a top frame and an upper-layer grating, the inner side of the square bottom frame is sleeved with the flexible lower-layer grating, the inner side of the square top frame is sleeved with the flexible upper-layer grating, and the wedge-shaped frame is arranged on the bottom frame. A series of mounting grooves are formed in the square top frame and used for assembling corresponding reagent bottles respectively, self-locking units are arranged in upper and lower grids respectively, through holes are formed in vertex angles of the upper and lower grids respectively, limiting columns are assembled on the vertex angles, blocking tables are arranged at the ends of the limiting columns, blind holes are formed in the middles of the upper and lower grids respectively, and guide columns are assembled on the blind holes; the upper portion and / or the lower portion of each guide column are / is connected with a spring, and when the upper-layer grating moves towards the lower-layer grating, the self-locking unit is triggered to lock the reagent bottles. According to the scheme, locking and unlocking operation of the reagent frame assembly is more convenient, and the working efficiency of operators can be improved.

Description

Technical Field

[0001] This invention belongs to the field of cyclotron oscillator technology, specifically relating to a culture medium cyclotron oscillator with a self-locking function. Background Technology

[0002] A cyclotron shaker is a common piece of equipment in biological laboratories, used to uniformly culture biological samples through oscillation. It typically consists of a main shaker unit and a reagent container assembly, where reagent bottles are placed. The cyclotron motion ensures uniform sample culture. Cyclotron shakers are widely used in biology, medicine, and other fields, such as cell culture and microbial culture experiments. However, existing cyclotron shakers generally have the following technical problems: reagent bottles are not securely fixed and are prone to accidental detachment during oscillation, leading to sample contamination or loss. The locking and unlocking operation of the reagent container assembly is inconvenient, requiring considerable manual operation and reducing work efficiency. The existing cyclotron shaker structure is relatively simple, lacking other functional modules such as compression mechanisms, and cannot meet the needs of more complex biological sample culture.

[0003] Therefore, existing cyclotron oscillators require further solutions. Summary of the Invention

[0004] To address some technical issues in existing rotary shakers regarding reagent bottle fixation, reagent frame assembly operation, and functional modules, this invention provides a culture medium rotary shaker that improves the ability of the reagent frame assembly to simultaneously lock and unlock multiple reagent bottles, making operation more convenient and increasing operator efficiency.

[0005] The solution to the technical problem of this invention is as follows: a culture medium rotary shaker is used, including a shaker main unit, a reagent frame assembly, a self-locking unit, and a wedge frame. The reagent frame assembly includes a bottom frame, a lower grid, a top frame, and an upper grid. A flexible lower grid is fitted inside the square bottom frame, and a flexible upper grid is fitted inside the square top frame. The square top frame is provided with a series of mounting slots for assembling corresponding reagent bottles. A self-locking unit is provided in each of the upper and lower grids. Through holes are provided at the top corners of the upper and lower grids, and limit posts are installed. A stop is provided at the end of the limit post. Blind holes are provided in the middle of the upper and lower grids, and guide posts are installed. A spring is connected to the upper and / or lower part of each guide post. When the upper grid moves to the lower grid, the self-locking unit is triggered to lock the reagent bottle.

[0006] Furthermore, the oscillator main unit includes a main housing, a control panel, and a rotary oscillation platform. An oscillator drive mechanism is installed inside the main housing, and the output shaft of the oscillator drive mechanism extends from the upper part of the main housing. A central shaft hole is provided at the bottom of the rotary oscillation platform, and the central shaft hole is fixedly installed together with the output shaft extension. A control panel is installed on one side of the main housing to facilitate the operation, setting, and display of the drive mechanism. A central shaft hole is provided at the bottom of the rotary oscillation platform for fixing the output shaft of the oscillator drive mechanism.

[0007] Furthermore, the self-locking unit includes a rectangular frame area, a pressure block, a strip frame area, and a wedge-shaped protrusion. The strip frame area is between adjacent rectangular frame areas, and the area between adjacent strip frame areas is solid or has a guide hole. An independent pressure block is provided on the side wall between the rectangular frame area and the strip frame area. Each pressure block has a wedge-shaped protrusion located in the strip frame area. During assembly, the wedge-shaped protrusions of the self-locking units located in the upper and lower grids are in opposite directions, that is, the inclined surfaces of each wedge-shaped protrusion are opposite.

[0008] Furthermore, a wedge frame is fitted between the upper and lower grids of the self-locking unit. The frame includes a wedge body, a conical part, a connecting plate, a limiting sleeve, and a guide sleeve. A series of transverse wedge bodies are fixed together by the connecting plate to form a transverse wedge body group, and a series of longitudinal wedge bodies are fixed together by the connecting plate to form a longitudinal wedge body group. The transverse wedge body groups and longitudinal wedge body groups are alternately distributed and fixed together by the connecting plate to form a square wedge frame. Each wedge body has a conical part on its upper and lower sides. A limiting sleeve is provided on the connecting plate at the four corners of the square wedge frame for fitting the limiting post, and a guide sleeve is provided on the connecting plate in the middle of the square wedge frame for fitting the guide post. During assembly, each wedge body is fitted into the strip frame area of ​​the upper and lower grids respectively.

[0009] Furthermore, it also includes a gyratory oscillation assembly, which includes a motor base plate, an eccentric disk, and a motor. The motor base plate is fixed to the inner wall of the main housing or an inner support. An eccentric disk is mounted on the motor shaft. An eccentric shaft is provided on the upper surface of the eccentric disk. The eccentric shaft is fixedly installed as an output shaft with the central shaft hole at the bottom of the gyratory oscillation platform.

[0010] Furthermore, it also includes a transverse and a longitudinal plate, both parallel to each other and attached to the back of the rotary oscillation platform. Longitudinal limiting posts are symmetrically fixed to the back of the rotary oscillation platform. The longitudinal plate has a hollowed-out area in the middle and longitudinal limiting grooves on both sides, with the longitudinal limiting posts located within the longitudinal limiting grooves. Similarly, transverse limiting posts are symmetrically fixed to the back of the longitudinal plate. The transverse plate has a hollowed-out area in the middle and transverse limiting grooves on both sides, with the transverse limiting posts located within the transverse limiting grooves. Through the constraint effect of the transverse and longitudinal plates, the rotary oscillation platform achieves rotary oscillation even when it cannot rotate.

[0011] Furthermore, a compression mechanism is provided to bring the upper and lower grids closer together. This mechanism includes a Z-shaped slide, a slider, and a connecting rod. Symmetrical Z-shaped slides are provided on the side walls of the bottom frame and the top frame, respectively. A slider is fitted into each Z-shaped slide. The upper and lower pairs of sliders are fixed together by the connecting rod. By moving the connecting rod to one side or the other side, the position of the pair of sliders in the Z-shaped slide can be changed, thereby changing the distance between the bottom frame and the top frame.

[0012] The beneficial effects of this invention are as follows: The technical solution of this culture medium rotary shaker has several advantages. The locking and unlocking operation of the reagent frame assembly is more convenient, improving operator efficiency. The reagent bottles are more securely fixed, preventing accidental detachment during shaking. The rotary shaking and compression mechanisms meet the needs of biological sample culture, improving the culture quality. This technical solution has a simple structure, is easy to manufacture, reduces production costs, and increases production efficiency. This technical solution can be widely applied in biology, medicine, and other fields, providing support for research and applications in these areas.

[0013] This approach also enhances the flexibility and functionality of cyclotron oscillators, allowing users to implement different amplitude modes to meet diverse sample culture needs. Furthermore, it enables precise control of the cyclotron oscillator amplitude, improving experimental repeatability and accuracy. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural schematic diagram of the cyclotron oscillator of the present invention; Figure 2 yes Figure 1 A schematic diagram of the structure of the reagent box assembly; Figure 3 This is a three-dimensional structural diagram of the wedge-shaped frame; Figure 4 yes Figure 1 Side view of the reagent box assembly; Figure 5 yes Figure 4 Schematic diagram of the cross-sectional structure of the middle AA section; Figure 6 This is a comparison diagram of the working states of the self-locking unit and the wedge frame; Figure 7 This is a partially enlarged schematic diagram of the self-locking unit; Figure 8 This is an assembly diagram of a type of cyclotron oscillator component.

[0015] Numbering in the diagram: 1. Oscillator main unit; 11. Main unit housing; 12. Control panel; 13. Rotary oscillation platform; 14. Central shaft hole; 2. Reagent frame assembly; 21. Bottom frame; 22. Lower grid; 23. Top frame; 24. Upper grid; 25. Mounting slot; 26. Limiting post; 27. Guide post; 3. Self-locking unit; 31. Rectangular frame area; 32. Pressure block; 33. Strip frame area; 34. Wedge-shaped protrusion; 35. Guide hole; 4. Wedge frame; 41. Wedge body; 4. Conical part. 2. Connecting plate 43, limiting sleeve 44, guide sleeve 45, rotary oscillation assembly 5, motor base plate 51, transverse plate 52, longitudinal plate 53, eccentric disk 54, motor 55, longitudinal limiting post 56, longitudinal limiting groove 57, transverse limiting post 58, transverse limiting groove 59, amplitude adjustment mechanism 6, radial slide 61, slider 62, adaptive eccentric shaft 63, limit set screw 64, compression mechanism 7, Z-shaped slide 71, slider 72, connecting rod 73. Implementation

[0016] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0017] Example 1: A kind of Figure 1 The culture medium rotary shaker shown is mainly an improvement on the existing reagent frame, which cannot lock the reagent bottle or has the problem of difficult reagent bottle locking operation. The rotary shaker mainly includes a shaker main unit 1, a reagent frame assembly 2, a self-locking unit 3, a wedge frame 4, and a rotary shaking assembly 5.

[0018] like Figure 1 As shown, the oscillator main unit 1 includes a main housing 11, a control panel 12, and a rotary oscillation platform 13. An oscillator drive mechanism is installed inside the main housing 11, and the output shaft of the oscillator drive mechanism extends from the upper part of the main housing 11. A central shaft hole 14 is provided at the bottom of the rotary oscillation platform 13, and this central shaft hole 14 is fixedly installed to the output shaft extension. The control panel 12 is installed on one side of the main housing 11 to facilitate the operation, setting, and display of the drive mechanism. The central shaft hole 14 at the bottom of the rotary oscillation platform 13 is used to fix the output shaft of the oscillator drive mechanism.

[0019] like Figure 2As shown, the reagent box assembly 2 includes a bottom frame 21, a lower grid 22, a top frame 23, an upper grid 24, mounting slots 25, limiting posts 26, and guide posts 27. A flexible lower grid 22 is fitted inside the square bottom frame 21, and a flexible upper grid 24 is fitted inside the square top frame 23. The square top frame 23 is provided with a series of mounting slots 25 for assembling corresponding reagent bottles. Each of the upper and lower grids has a self-locking unit 3. Through holes are provided at the top corners of the upper and lower grids, and limiting posts 26 are fitted thereon. A stop is provided at the end of each limiting post 26. Blind holes are provided in the middle sections of the upper and lower grids, and guide posts 27 are fitted thereon. A spring is connected to the upper and / or lower part of each guide post 27. When the upper grid 24 moves towards the lower grid 22, the self-locking unit 3 is triggered to lock the reagent bottle.

[0020] like Figure 7 As shown, the self-locking unit 3 includes a rectangular frame area 31, a pressure block 32, a strip frame area 33, a wedge-shaped protrusion 34, and a guide hole 35. The strip frame area 33 is located between adjacent rectangular frame areas 31, and the space between adjacent strip frame areas 33 is either solid or has a guide hole 35. An independent pressure block 32 is provided on the sidewall between the rectangular frame area 31 and the strip frame area 33, and each pressure block 32 has a wedge-shaped protrusion 34 located within the strip frame area 33.

[0021] During assembly, the wedge-shaped protrusions 34 of the self-locking units 3 located in the upper grille 24 and the lower grille 22 are in opposite directions, that is, the inclined surfaces of each wedge-shaped protrusion 34 are opposite.

[0022] like Figure 3 As shown, the wedge frame 4 includes a wedge body 41, a tapered portion 42, a connecting plate 43, a limiting sleeve 44, and a guide sleeve 45. A series of transverse wedge bodies 41 are fixed together by the connecting plate 43 to form a transverse wedge body group, and a series of longitudinal wedge bodies are fixed together by the connecting plate to form a longitudinal wedge body group. The transverse wedge body groups and longitudinal wedge body groups are alternately distributed and fixed together by the connecting plate, ultimately forming a square wedge frame 4. Each wedge body has a tapered portion 42 on its upper and lower sides. Limiting sleeves 44 are provided on the connecting plates at the four apex corners of the square wedge frame 4 for fitting the limiting posts 26, and guide sleeves 45 are provided on the connecting plate in the middle of the square wedge frame 4 for fitting the guide posts 27. During assembly, each wedge body 41 is fitted into the strip frame area 33 of the upper and lower layers of the grid.

[0023] The rotary oscillation assembly 5 includes a motor base plate 51, a transverse plate 52, a longitudinal plate 53, an eccentric disk 54, a motor 55, a longitudinal limiting post 56, a longitudinal limiting groove 57, a transverse limiting post 58, and a transverse limiting groove 59. The motor base plate 51 is fixed to the inner wall of the main housing 11 or the inner bracket. The eccentric disk 54 is mounted on the rotating shaft of the motor 55. An eccentric shaft is provided on the upper surface of the eccentric disk 54. The eccentric shaft serves as the output shaft and is fixedly installed in the central shaft hole 14 at the bottom of the rotary oscillation platform 13.

[0024] It also includes a transverse plate 52 and a longitudinal plate 53, which are parallel to and attached to the back of the rotary oscillation platform 13. A longitudinal limiting post 56 is symmetrically fixed to the back of the rotary oscillation platform 13. A hollow area is provided in the middle of the longitudinal plate 53, and longitudinal limiting grooves 57 are provided on both sides. The longitudinal limiting post 56 is located within the longitudinal limiting groove 57. A transverse limiting post 58 is symmetrically fixed to the back of the longitudinal plate 53. A hollow area is provided in the middle of the transverse plate 52, and transverse limiting grooves 59 are provided on both sides. The transverse limiting post 58 is located within the transverse limiting groove 59. Through the constraint effect of the transverse plate 52 and the longitudinal plate 53, the rotary oscillation platform 13 can achieve rotary oscillation without being able to rotate.

[0025] A compression mechanism is provided to bring the upper and lower grids closer together. For example, a counterweight can be added to the upper grid, or a screw can be passed through the bottom frame 21 and the top frame 23 to compress them inward. Figure 1 The image shows a compression mechanism 7, which includes a Z-shaped slide rail 71, a slider 72, and a connecting rod 73. Symmetrical Z-shaped slide rails 71 are respectively provided on the side walls of the bottom frame 21 and the top frame 23. A slider 72 is fitted into each Z-shaped slide rail 71. The upper and lower pairs of sliders 72 are fixed together by the connecting rod 73. By moving the connecting rod 73 to one side or the other side, the position of the pair of sliders 72 in the Z-shaped slide rail 71 can be changed, thereby changing the distance between the bottom frame 21 and the top frame 23.

[0026] The aforementioned culture medium rotary shaker is used for culturing biological samples such as cells or colonies. The main improvement of this device lies in solving the problem of existing reagent frames not being able to lock reagent bottles or the difficulty in locking reagent bottles. The operation of this culture medium rotary shaker is relatively simple; just place the reagent bottle in the reagent frame assembly and start the shaker. Simultaneously, the locking and unlocking operation of the reagent frame assembly is also very convenient, meeting the needs of biological sample culture. The usage process is as follows: First, place the reagent frame assembly on the rotary shaking platform of the shaker, ensuring an appropriate distance between the reagent frame assembly and the rotary shaking platform. Then, place the biological sample to be cultured in the reagent bottle and insert the reagent bottle into the mounting slot of the reagent frame assembly. After the reagent bottle is placed, the self-locking units in the upper and lower grids will automatically lock the reagent bottle, ensuring its fixation. Set the control panel of the shaker as needed, including parameters such as time and speed. Start the shaker to begin rotary oscillation. During oscillation, the rotary oscillation assembly causes the reagent frame assembly to perform rotary oscillation motion, thereby ensuring uniform sample culture. If it is necessary to stop the shaker, it can be done through the control panel. Meanwhile, the reagent box assembly can be unlocked to remove the reagent bottle.

[0027] Furthermore, an amplitude adjustment mechanism 6 can be added. This mechanism has a radial groove 61 on the upper surface of the eccentric disk 54, and a slider 62 is fitted inside the radial groove 61. The slider 62 is fixed with an adaptive eccentric shaft 63. Simultaneously, screw holes are installed on the transverse and longitudinal sidewalls of the main housing, and limit screws 64 are installed on them respectively. The inner end of the transverse limit screw corresponds to the sidewall of the transverse plate 52, and the inner end of the longitudinal limit screw corresponds to the sidewall of the longitudinal plate 53. When the transverse limit screw and / or the limit screw are adjusted, the extreme offset positions of the transverse plate 52 and / or the longitudinal plate 53 can be limited. When the extreme positions of the transverse plate 52 and the longitudinal plate 53 are constrained, the range of movement of each limiting post and the corresponding limiting groove is first limited, and then the range of movement of the slider 62 within the radial groove 61 is limited, that is, the degree of eccentricity of the eccentric shaft 63 is limited. This allows for the constraint of the lateral and longitudinal amplitudes of the cyclotron oscillation platform 13, enabling different modes such as large-diameter cyclotron oscillation, small-diameter cyclotron oscillation, and elliptical cyclotron oscillation. The addition of the amplitude adjustment mechanism 6 allows users to achieve different amplitude modes as needed, thereby meeting diverse sample culture requirements and improving the application flexibility and functionality of the cyclotron oscillator.

[0028] In practical use, users can adjust the amplitude of the gyroscopic oscillator by adjusting the lateral and longitudinal limit screws. Specifically, when a large-diameter gyroscopic oscillation is required, the user can adjust the lateral and longitudinal limit screws to widen the range of movement of the limiting post and its corresponding limiting groove, allowing the transverse plate 52 and longitudinal plate 53 to have a larger range of movement, thereby widening the range of movement of the slider 62 within the radial groove 61, thus achieving a larger amplitude. When a small-diameter gyroscopic oscillation is required, the lateral and longitudinal limit screws need to be adjusted to narrow the range of movement of the limiting post and its corresponding limiting groove, reducing the range of movement of the slider 62 within the radial groove 61, thus achieving a smaller amplitude.

[0029] In addition, depending on the specific circumstances, users can also achieve other different amplitude modes, such as elliptical cyclotron oscillation, by adjusting the limit set screw and / or amplitude adjustment mechanism.

[0030] The specific embodiments described above are merely illustrative or explanatory of the principles of the present invention and do not constitute a limitation thereof. Therefore, any modifications, equivalent substitutions, improvements, etc., made without departing from the spirit and scope of the present invention should be included within the protection scope of the present invention.

Claims

1. A culture medium rotary shaker, comprising a shaker main unit (1) and a reagent box assembly (2), characterized in that, The reagent frame assembly (2) includes a bottom frame (21), a lower grid (22), a top frame (23), and an upper grid (24). The square bottom frame (21) is fitted with a flexible lower grid (22), and the square top frame (23) is fitted with a flexible upper grid (24). The square top frame (23) is provided with a series of mounting slots (25) for assembling corresponding reagent bottles. The upper and lower grids are respectively provided with self-locking units (3). The top corners of the upper and lower grids are respectively provided with through holes and fitted with limit posts (26). The ends of the limit posts (26) are provided with baffles. The middle parts of the upper and lower grids are respectively provided with blind holes and fitted with guide posts (27). The upper and / or lower parts of each guide post (27) are connected with springs. When the upper grid (24) moves to the lower grid (22), the self-locking unit (3) is triggered to lock the reagent bottle.

2. The culture medium vortex shaker according to claim 1, characterized in that, The oscillator main unit (1) includes a main unit housing (11), a control panel (12), and a rotary oscillation platform (13). An oscillator drive mechanism is installed inside the main unit housing (11). The output shaft of the oscillator drive mechanism is led out from the upper part of the main unit housing (11). A central shaft hole (14) is provided at the bottom of the rotary oscillation platform (13). The central shaft hole (14) is fixedly installed together with the output shaft lead-out part. A control panel (12) is installed on one side of the main unit housing (11) to facilitate the operation and display of the drive mechanism. A central shaft hole (14) is provided at the bottom of the rotary oscillation platform (13) for fixing the output shaft of the oscillator drive mechanism.

3. The culture medium vortex shaker according to claim 1, characterized in that, The self-locking unit (3) includes a rectangular frame area (31), a pressure block (32), a strip frame area (33), and a wedge protrusion (34). The adjacent rectangular frame areas (31) are separated by a strip frame area (33), and the adjacent strip frame areas (33) are solid or have guide holes (35). An independent pressure block (32) is provided on the side wall between the rectangular frame area (31) and the strip frame area (33). Each pressure block (32) has a wedge protrusion (34) located in the strip frame area (33). During assembly, the wedge protrusions (34) of the self-locking unit (3) located in the upper grille (24) and the lower grille (22) are in opposite directions, that is, the inclined surfaces of each wedge protrusion (34) are opposite.

4. The culture medium vortex shaker according to claim 3, characterized in that, A wedge frame (4) is fitted between the upper and lower grids of the self-locking unit (3). The wedge frame (4) includes a wedge body (41), a cone part (42), a connecting plate (43), a limiting sleeve (44), and a guide sleeve (45). A series of transverse wedge bodies (41) are fixed by the connecting plate (43) to form a transverse wedge body group. A series of longitudinal wedge bodies are fixed by the connecting plate to form a longitudinal wedge body group. The transverse wedge body group and the longitudinal wedge body group are alternately distributed and fixed together by the connecting plate to finally form a square wedge frame (4). Each wedge body has a cone part (42) on its upper and lower sides. A limiting sleeve (44) is set on the connecting plate at the four corners of the square wedge frame (4) to fit the limiting post (26). A guide sleeve (45) is set on the connecting plate in the middle of the square wedge frame (4) to fit the guide post (27). During assembly, each wedge body (41) is matched and fitted into the strip frame area (33) of the upper and lower grids.

5. The culture medium vortex shaker according to claim 2, characterized in that, It also includes a gyratory oscillation assembly (5), which includes a motor base plate (51), an eccentric disk (54) and a motor (55). The motor base plate (51) is fixed to the inner wall or inner support of the main housing (11). An eccentric disk (54) is installed on the rotating shaft of the motor (55). An eccentric shaft is provided on the upper surface of the eccentric disk (54). The eccentric shaft is fixedly installed as an output shaft with the central shaft hole (14) at the bottom of the gyratory oscillation platform (13).

6. The culture medium vortex shaker according to claim 5, characterized in that, It also includes a transverse plate (52) and a longitudinal plate (53), which are parallel to each other and attached to the back of the rotary oscillation platform (13). A longitudinal limiting post (56) is symmetrically fixed on the back of the rotary oscillation platform (13). A hollow area is set in the middle of the longitudinal plate (53), and longitudinal limiting grooves (57) are set on both sides. The longitudinal limiting post (56) is located in the longitudinal limiting groove (57). A transverse limiting post (58) is symmetrically fixed on the back of the longitudinal plate (53). A hollow area is set in the middle of the transverse plate (52), and transverse limiting grooves (59) are set on both sides. The transverse limiting post (58) is located in the transverse limiting groove (59). Through the constraint of the transverse plate (52) and the longitudinal plate (53), the rotary oscillation platform (13) can achieve rotary oscillation without being able to rotate.

7. The culture medium vortex shaker according to claim 3 or 4, characterized in that, A compression mechanism (7) is provided to bring the upper and lower grids closer together. It includes a Z-shaped slide (71), a slider (72) and a connecting rod (73). Symmetrical Z-shaped slides (71) are provided on the side walls of the bottom frame (21) and the top frame (23). A slider (72) is fitted in each Z-shaped slide (71). The upper and lower pairs of sliders (72) are fixed together by the connecting rod (73). By moving the connecting rod (73) to one side or the other side, the position of the pair of sliders (72) in the Z-shaped slide (71) can be changed, thereby changing the distance between the bottom frame (21) and the top frame (23).

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