Liquid separation device for automatic library construction

Abstract

The invention discloses a liquid separation device for automatic library construction, which comprises a patch board, the upper surface of which is provided with a plurality of fluid interfaces communicated with an external injection pump or an air source through conduits; the temperature control structure comprises a low-temperature control area and a normal-temperature control area which are arranged in a mutual heat insulation manner The interface board comprises a laminated micro-fluidic structure of an upper-layer plate, a middle film and a lower-layer plate, the upper-layer plate is provided with a reagent injection port and an air valve interface, the middle film forms a micro-valve which can be controlled to be opened and closed under the action of air pressure, the lower-layer plate forms at least one metering micro-channel and a transmission micro-channel, and a quantitative volume of reagent is injected into the digital micro-fluidic chip through a liquid injection connector at the downstream. The device is compact in structure, reliable in sealing, high in modularization degree and suitable for gene sequencing library construction and related microfluidic automation application.

Description

Technical Field

[0001] This disclosure relates to the fields of microfluidics and automated liquid dispensing technology, and more specifically, to a liquid dispensing device for automated library construction. Background Technology

[0002] In gene sequencing and other biochemical analyses, library construction is a core step in sample pretreatment. This process typically requires adding various reagents sequentially to the reaction system in different volume ratios.

[0003] Traditional library construction relies heavily on manual pipetting or large automated equipment. Manual operations are cumbersome, prone to errors, and carry a high risk of contamination; while large library construction equipment is bulky, complex to install, and costly to maintain, making it unsuitable for widespread use in research laboratories or small centers.

[0004] Currently, microfluidic chips (including continuous flow and digital microfluidic (DMF)) have significant advantages in precision liquid dispensing and reaction control, enabling precise manipulation of micro-level reagents. However, existing dispensing devices still suffer from the following problems in the liquid path control between the microfluidic chip and the reagent storage unit: 1. Lack of modular structural design, making maintenance and replacement inconvenient; 2. Reagent storage and temperature control functions are not integrated, making it impossible to stably store low-temperature and room-temperature reagents simultaneously; 3. Insufficient sealing and reliability of the puncture-type connection structure; 4. Dispersed mixing and quantitative functions, resulting in complex liquid flow paths, which is not conducive to high-throughput automation.

[0005] Therefore, one or more methods are needed to solve the above problems.

[0006] It should be noted that the information disclosed in the background section above is only used to enhance the understanding of the background of this disclosure, and therefore may include information that does not constitute prior art known to those skilled in the art. Summary of the Invention

[0007] The purpose of this disclosure is to provide a dispensing apparatus for automated library construction, thereby overcoming, at least to some extent, one or more problems caused by limitations and defects in related technologies.

[0008] To achieve the above objectives, the present invention provides a liquid dispensing device for automated library construction, comprising:

[0009] The power strip has several fluid interfaces on its upper surface for connecting to an external injection pump or gas source via conduits.

[0010] The temperature control structure includes a low-temperature control zone and a normal-temperature control zone that are thermally insulated from each other;

[0011] The interface board comprises a stacked microfluidic structure consisting of an upper plate, an intermediate membrane, and a lower plate. The upper plate is provided with a reagent injection port and a gas valve interface. The intermediate membrane forms a controllable microvalve under gas pressure. The lower plate forms at least one metering microchannel and one transmission microchannel. A quantitative volume of reagent is injected into the digital microfluidic chip downstream through a liquid injection connector.

[0012] Furthermore, the power strip includes an upper power strip and multiple mixing chamber power strips. Each power strip is provided with a Luer interface as a fluid interface, and every two upper surface interfaces correspond to a lower surface piercing connection structure, which is used to pierce the sealing film of the reagent storage bottle and seal and communicate with the channel inside the power strip.

[0013] Furthermore, the temperature control structure is located inside the outer shell, and the outer shell forms a supporting and sealing cavity for the device; the temperature control structure includes a low-temperature control area and a normal-temperature control area with an embedded temperature control module, and the two areas are arranged insulated from each other; each area is provided with a slot for placing a reagent storage bottle and a groove for installing a mixing chamber, and the bottle mouth of the reagent storage bottle is arranged opposite to the piercing structure on the lower surface of the power strip or the piercing mechanism on the upper plate of the mixing chamber to achieve liquid circuit communication.

[0014] Furthermore, the socket board and the temperature control structure are connected by a cover-and-close structure. The socket board can be opened and closed with the outer shell through a hinge. When the socket board is closed down to the temperature control structure, the piercing structure on its lower surface automatically pierces the sealing film of the reagent storage bottle located in the slot and maintains a sealed connection in the closed state.

[0015] Furthermore, the interface board is located below the temperature control structure, and its upper plate body is provided with a positioning structure for positioning in conjunction with corresponding parts of the temperature control structure and the outer shell.

[0016] The reagent injection port of the interface board is connected to the connector plate or mixing chamber via a conduit.

[0017] Furthermore, the temperature control structure includes a mixing chamber, which is connected to the liquid path of the reagent storage bottle.

[0018] The mouth of the reagent storage bottle is sealed to the puncture mechanism of the upper plate of the mixing chamber via a conduit or by direct puncture.

[0019] The mixing chamber includes a mixing chamber temperature control plate and a mixing chamber interface plate. The mixing chamber interface plate includes an upper plate, a middle membrane, and a lower plate.

[0020] The upper plate is equipped with a piercing mechanism for piercing the seal of the reagent bottle and a gas pipe interface for connecting to the gas source;

[0021] The middle membrane has through-holes to connect to the liquid passage;

[0022] The lower plate has channels for reagent mixing, quantification, or delivery.

[0023] The mixing chamber is connected to the interface plate liquid path via a conduit, enabling the continuous distribution of reagents from the storage bottle through the mixing chamber to the interface plate.

[0024] Furthermore, the upper plate of the interface board is also provided with a sampling port and a injection port. The outer edges of the sampling port and the injection port are provided with guide surfaces so that the pipetting chamber can enter the chip through the guide surfaces for sampling and injection.

[0025] Furthermore, the intermediate membrane is connected to the gas source conduit of the power strip via a gas valve interface, and valve control is achieved under external gas pressure control to control the opening and closing of the metering channel of the lower plate, thereby realizing metering, path switching and backflow prevention functions.

[0026] Furthermore, the lower plate is provided with a first groove, a second groove, and a third groove:

[0027] The first channel is located below the reagent injection port and is connected to the reagent storage bottle;

[0028] The second channel includes several metering branches of different volumes, which can be selected for use by different joint positions on the upper plate;

[0029] The third channel is connected to the liquid injection connector and is used to output quantitative reagents to the downstream chip.

[0030] The downstream of the second channel may be equipped with an overflow port connected to the waste liquid tank to discharge excess reagent in order to maintain measurement accuracy.

[0031] Furthermore, the power strip, temperature control structure, mixing chamber, and interface board are all covered by an outer shell and interconnected through conduits, tracheal connectors, and positioning structures to form a detachable modular assembly. The temperature control structure is located in the middle layer and is used to provide reagent storage and temperature control functions. The power strip is located above it and completes the puncture operation through a closing action. The mixing chamber is located inside the temperature control structure and is directly connected to the reagent storage bottle. The interface board is located below it to form a liquid output path, thus constituting an upper and lower stacked automatic liquid dispensing system.

[0032] This disclosure utilizes a modular design of power strips, temperature control structures, and interface boards to achieve independent storage and on-demand distribution of reagents in different temperature zones. It can automatically establish liquid circuit connections through the stacked configuration of power strips, temperature control structures, and interface boards, resulting in a compact structure and low risk of contamination.

[0033] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit this disclosure. Attached Figure Description

[0034] The above and other features and advantages of this disclosure will become more apparent from the detailed description of exemplary embodiments thereof with reference to the accompanying drawings.

[0035] Figure 1and 2 A schematic diagram of a dispensing apparatus for automated library construction according to an exemplary embodiment of the present disclosure is shown;

[0036] Figure 3 and 4 A schematic diagram of the structure of an interface board according to an exemplary embodiment of the present disclosure is shown;

[0037] Figure 5 A schematic diagram of the lower layer board structure of an interface board according to an exemplary embodiment of the present disclosure is shown.

[0038] Figure 6 A schematic diagram of a mixing chamber according to an exemplary embodiment of the present disclosure is shown. Detailed Implementation

[0039] Exemplary embodiments will now be described more fully with reference to the accompanying drawings. However, these exemplary embodiments can be implemented in many forms and should not be construed as limited to the embodiments set forth herein; rather, they are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the exemplary embodiments to those skilled in the art. The same reference numerals in the drawings denote the same or similar parts, and therefore repeated descriptions of them will be omitted.

[0040] Furthermore, the described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. Numerous specific details are provided in the following description to give a thorough understanding of embodiments of this disclosure. However, those skilled in the art will recognize that the technical solutions of this disclosure can be practiced without one or more of the specific details described, or other methods, components, materials, apparatuses, steps, etc., can be employed. In other instances, well-known structures, methods, apparatuses, implementations, materials, or operations are not shown or described in detail to avoid obscuring various aspects of this disclosure.

[0041] The block diagrams shown in the accompanying drawings are merely functional entities and do not necessarily correspond to physically independent entities. That is, these functional entities can be implemented in software, or in one or more software-hardened modules, or in different network and / or processor devices and / or microcontroller devices.

[0042] In this example embodiment, as Figure 1-4 As shown, the liquid dispensing device for automated library construction of the present invention includes:

[0043] The power strip 2 has several fluid interfaces on its upper surface for connecting to an external injection pump or gas source via conduits.

[0044] The temperature control structure 4 includes a low-temperature control zone 9 and a normal-temperature control zone 15 that are arranged in a heat-insulated manner.

[0045] The interface board 14 includes a stacked microfluidic structure comprising an upper plate 29, an intermediate membrane 30, and a lower plate 31. The upper plate 29 is provided with a reagent injection port and a gas valve interface. The intermediate membrane 30 forms a controllable microvalve under gas pressure. The lower plate 31 forms at least one metering microchannel and one transmission microchannel. A quantitative volume of reagent is injected into the digital microfluidic chip downstream through the liquid injection connector.

[0046] This example uses a modular design of power strips, temperature control structures, and interface boards to achieve independent storage and on-demand distribution of reagents in different temperature zones. It can automatically establish liquid circuit connections through the stacked configuration of power strips, temperature control structures, and interface boards, resulting in a compact structure and low risk of contamination.

[0047] In one embodiment of the present invention, the connector 2 includes an upper connector 1 and multiple mixing chamber connectors 5, 6, 7, and 8. Each connector is provided with a Luer interface as a fluid interface, and every two upper surface interfaces correspond to a lower surface piercing connection structure for piercing the sealing film of the reagent storage bottle 13 and sealingly communicating with the channel inside the connector. The upper connector 1 has a vertical channel inside and a piercing structure designed on its lower surface. The mixing chamber connectors 5, 6, 7, and 8 have the same features as the upper connector 1.

[0048] In one embodiment of the present invention, the temperature control structure 4 is disposed inside the outer shell, and the outer shell forms a supporting and sealing cavity of the device; the temperature control structure 4 includes a low temperature control zone 9 and a normal temperature control zone 15 embedded with a temperature control module, and the two zones are arranged insulated from each other; each zone is provided with a slot 39 for placing a reagent storage bottle 13 and a groove for installing mixing chambers 10, 11, and 12, and the bottle mouth of the reagent storage bottle 13 is arranged opposite to the piercing structure on the lower surface of the power strip 2 or the piercing mechanism on the upper plate of the mixing chambers 10, 11, and 12 to achieve liquid circuit communication.

[0049] In one embodiment of the present invention, the plug-in board 2 and the temperature control structure 4 are connected by a cover-and-close structure. The plug-in board 2 is hinged to the outer shell and can be opened and closed. When the plug-in board 2 is closed down to the temperature control structure 4, the piercing structure on its lower surface automatically pierces the sealing film of the reagent storage bottle 13 located in the slot and maintains a sealed connection in the closed state.

[0050] In one embodiment of the present invention, the interface plate 14 is disposed below the temperature control structure 4, and its upper plate 29 is provided with a positioning structure for cooperating with corresponding parts of the temperature control structure 4 and the outer shell for positioning; the positioning structure consists of positioning holes, positioning angles, and snap-fit ​​structures provided on the upper plate 29. The reagent injection port of the interface plate 14 is connected to the plug plate 2 or the mixing chambers 10, 11, and 12 via a conduit.

[0051] In one embodiment of the present invention, see Figure 6The temperature control structure 4 is provided with mixing chambers 10, 11, and 12, which are connected to the liquid path of the reagent storage bottle 13.

[0052] The mouth of the reagent storage bottle 13 is sealed to the puncture mechanism of the upper plate of the mixing chamber via a conduit or by direct puncture.

[0053] The mixing chambers 10, 11, and 12 include a mixing chamber temperature control plate 36 and a mixing chamber interface plate. The mixing chamber interface plate includes an upper plate 37, a middle membrane 38, and a lower plate 41.

[0054] The upper plate 37 is equipped with a piercing mechanism for piercing the sealing film of the reagent bottle and a gas pipe interface 35 for connecting to the gas source;

[0055] The middle membrane 38 is provided with through holes to connect to the liquid passage;

[0056] The lower plate 41 is provided with channels for reagent mixing, quantification, or delivery.

[0057] Mixing chambers 10, 11, and 12 are connected to the liquid path of interface plate 14 via conduits, enabling continuous distribution of reagents from storage bottles through the mixing chambers to interface plate 14. The temperature control plate 36 of the mixing chamber is designed with a reagent bottle recess 39 and a gas tube connector recess 40 for placing the reagent storage bottle 13 and the gas tube connector 35.

[0058] In one embodiment of the present invention, the upper plate of the interface board 14 is further provided with a sampling port 16 and a sample injection port 17. The outer edges of the sampling port 16 and the sample injection port 17 are provided with guide surfaces so that the pipetting chamber can enter the chip for sampling and injection through the guide surfaces. In this example, the sampling port 16 is designed with a square hole on its surface and has a beveled outer side so that it can be tilted at a large angle to enter the downstream chip for sampling when using a pipette; the sample injection port 17 is consistent with the sampling port 16, so that the sample can be injected into the downstream chip at a large angle when using a pipette. In addition, the upper plate 14 is also provided with a positioning hole 22, a plug port 24, a gas valve interface 25, a positioning angle 23, a connector port 18, a reagent injection port 19, a hot zone opening 20, an absorbent material opening 21, and a buckle 28. The positioning holes 22 have through holes at the four corners of the upper plate 29 to align the intermediate membrane 30 and the lower plate 31; the plug port 2 has a corresponding plug interface, allowing a specific type of plug to be inserted into the plug port to block the channel; the air valve interface 25 has a corresponding air valve interface, allowing a specific air valve to be inserted into the interface to connect the intermediate membrane 30 to the air duct in the upper wiring board 1; the positioning angle 23 has a corner cut off from the upper plate 29 to form a fixed direction. The reagent injection port 19 has a volcano-shaped puncture opening on its surface, which can puncture the film on the underside of the reagent storage bottle 13; the hot zone opening 20 has an opening in the center, allowing the external temperature control structure to be directly attached to the downstream chip; the absorbent material opening 21 has circular openings symmetrically located on both sides, which can hold cylindrical absorbent material; the buckle 28 has a groove on its side, allowing the outer shell to cover the entire upper plate 29, the intermediate membrane 30, the lower plate 31, and the chip.

[0059] In one embodiment of the present invention, the intermediate membrane 30 is connected to the gas source conduit of the power strip via a gas valve interface, and valve control is achieved under external gas pressure to control the opening and closing of the metering channel of the lower plate 31, realizing metering, path switching and backflow prevention functions. The intermediate membrane 30 corresponds to the opening on the upper plate 29, and no opening is provided below the gas valve interface 25.

[0060] In one embodiment of the present invention, see Figure 5 As shown, the lower plate 31 is provided with a first channel 32, a second channel 33 and a third channel 34:

[0061] The first channel 32 is located below the reagent injection port 19 and is connected to the reagent storage bottle 13;

[0062] The second channel 33 includes several metering branches of different volumes, which can be selected for use through different joint positions on the upper plate 29;

[0063] The third channel 34 is connected to the liquid injection connector 26 and is used to output quantitative reagents to the downstream chip.

[0064] The downstream of the second channel 33 may be provided with an overflow port connected to the waste liquid pool to discharge excess reagent in order to maintain measurement accuracy.

[0065] In one embodiment of the present invention, the power strip 2, temperature control structure 4, mixing chambers 10, 11, 12 and interface plate 14 are all covered by an outer shell and connected to each other through conduits, tracheal connectors and positioning structures to form a detachable modular combination; the temperature control structure 4 is located in the middle layer and is used to provide reagent storage and temperature control functions; the power strip 2 is located above it and completes the puncture operation through the closing action; the mixing chambers 10, 11, 12 are located inside the temperature control structure 4 and are directly connected to the reagent storage bottle 13; the interface plate 14 is located below it to form a liquid output path, thereby constituting an upper and lower stacked automatic liquid dispensing system.

[0066] To enable those skilled in the art to more clearly understand the solution of the present invention, the embodiments of the present invention are described below:

[0067] The operation process of the liquid separation device of the present invention is as follows: rotate the rotating shaft 3 to open the upper plate 2, open the ambient temperature control area 15 and the low temperature control area 9, place the interface board chip integrated structure 14 on the base 4, cover the ambient temperature control area 15 and the control board 9, and then close the upper plate 2 to carry out the automatic liquid separation process.

[0068] For the library construction reaction in sequencing, this dispensing and storage device is used. First, 33 reagent storage bottles are categorized by room temperature and low temperature and placed in 33 recesses. Reagents requiring room temperature storage are placed in the room temperature control zone, and those requiring low temperature storage are placed in the low temperature control zone. Each interface on the upper connector is connected to the syringe pump via tubing. After closing the upper connector, each hole corresponds to a reagent storage bottle. The membrane of the reagent storage bottle is then punctured. According to the reaction flow, tubing is used at the interfaces on the upper plate to connect the channels of the lower plate for different quantitative volumes. For example, connecting the outlet of the first channel to the fourth inlet of the second channel ensures that the reagent volume flowing into the downstream chip is 1 μL each time. Similarly, connecting the second channel at different locations allows different volumes (2 μL, 3 μL, 4 μL) to flow in. L); The quantitative process is as follows: After the reagent passes through the first and second channels, it fills both channels. There is an outlet at the outlet that connects to the waste liquid pool. The reagent will overflow and flow into the waste liquid pool, thus ensuring that the quantitative channel contains quantitative reagent. Then, the injection pump is controlled to push another reagent, causing the other reagent to overflow. Both reagents flow out from the same outlet, i.e., the injection connector, to the downstream chip. Thus, the injection pump and the dispensing chip achieve automated dispensing, which can both quantify and prevent contamination.

[0069] In summary, this invention can be applied to biochemical fields requiring precise dispensing of multiple reagents, such as gene sequencing library construction, nucleic acid detection, and immune reaction system setup. Its modular and automated design significantly reduces contamination risks and operational complexity, making it highly valuable for industrial application and scientific research.

[0070] It should be noted that although the steps of the method in this disclosure are described in a specific order in the accompanying drawings, this does not require or imply that the steps must be performed in that specific order, or that all the steps shown must be performed to achieve the desired result. Additional or alternative steps may be omitted, multiple steps may be combined into one step, and / or a step may be broken down into multiple steps.

[0071] Furthermore, the above figures are merely illustrative of the processes included in the method according to exemplary embodiments of the present invention, and are not intended to be limiting. It is readily understood that the processes shown in the above figures do not indicate or limit the temporal order of these processes. Additionally, it is readily understood that these processes may be executed synchronously or asynchronously, for example, in multiple modules.

[0072] Other embodiments of this disclosure will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not disclosed herein. The specification and embodiments are to be considered exemplary only, and the true scope and spirit of this disclosure are indicated by the claims.

[0073] It should be understood that this disclosure is not limited to the precise structures described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of this disclosure is limited only by the appended claims.

Claims

1. A dispensing device for automated library construction, characterized in that, include: The power strip has several fluid interfaces on its upper surface for connecting to an external injection pump or gas source via conduits. The temperature control structure includes a low-temperature control zone and a normal-temperature control zone that are thermally insulated from each other; The interface board comprises a stacked microfluidic structure consisting of an upper plate, an intermediate membrane, and a lower plate. The upper plate is provided with a reagent injection port and a gas valve interface. The intermediate membrane forms a controllable microvalve under gas pressure. The lower plate forms at least one metering microchannel and one transmission microchannel. A quantitative volume of reagent is injected into the digital microfluidic chip downstream through a liquid injection connector.

2. The liquid separation device according to claim 1, characterized in that, The connector includes an upper connector and multiple mixing chamber connectors. Each connector is provided with a Luer interface as a fluid interface, and every two upper surface interfaces correspond to a lower surface piercing connection structure, which is used to pierce the sealing film of the reagent storage bottle and seal and communicate with the channel inside the connector.

3. The liquid separation device according to claim 1 or 2, characterized in that, The temperature control structure is located inside the outer shell, and the outer shell forms a supporting and sealing cavity for the device. The temperature control structure includes a low-temperature control zone and a normal-temperature control zone with an embedded temperature control module, and the two zones are arranged insulated from each other. Each zone is provided with a slot for placing a reagent storage bottle and a groove for installing a mixing chamber. The bottle mouth of the reagent storage bottle is arranged opposite to the piercing structure on the lower surface of the power strip or the piercing mechanism on the upper plate of the mixing chamber to achieve liquid circuit communication.

4. The liquid separation device according to claim 3, characterized in that, The socket and the temperature control structure are connected by a cover-and-close structure. The socket can be opened and closed with the outer shell by a hinge. When the socket is closed down to the temperature control structure, the piercing structure on its lower surface automatically pierces the sealing film of the reagent storage bottle located in the slot and maintains a sealed connection in the closed state.

5. The liquid separation device according to claim 1, characterized in that, The interface board is located below the temperature control structure, and its upper plate body is provided with a positioning structure for positioning in conjunction with the corresponding parts of the temperature control structure and the outer shell. The reagent injection port of the interface board is connected to the connector plate or mixing chamber via a conduit.

6. The liquid separation device according to claim 1, characterized in that, The temperature control structure includes a mixing chamber, which is connected to the liquid path of the reagent storage bottle. The mouth of the reagent storage bottle is sealed to the puncture mechanism of the upper plate of the mixing chamber via a conduit or by direct puncture. The mixing chamber includes a mixing chamber temperature control plate and a mixing chamber interface plate. The mixing chamber interface plate includes an upper plate, a middle membrane, and a lower plate. The upper plate is equipped with a piercing mechanism for piercing the seal of the reagent bottle and a gas pipe interface for connecting to the gas source; The middle membrane has through-holes to connect to the liquid passage; The lower plate has channels for reagent mixing, quantification, or delivery. The mixing chamber is connected to the interface plate liquid path via a conduit, enabling the continuous distribution of reagents from the storage bottle through the mixing chamber to the interface plate.

7. The liquid separation device according to claim 1, characterized in that, The upper plate of the interface board is also provided with a sampling port and a sample injection port. The outer edges of the sampling port and the sample injection port are provided with guide surfaces so that the pipetting chamber can enter the chip through the guide surfaces for sampling and injection.

8. The liquid separation device according to claim 1, characterized in that, The intermediate membrane is connected to the gas source conduit of the power strip through a gas valve interface. Under the control of external air pressure, the valve is controlled to open and close the metering channel of the lower plate, realizing metering, path switching and backflow prevention functions.

9. The liquid separation device according to claim 1, characterized in that, The lower plate is provided with a first groove, a second groove and a third groove: The first channel is located below the reagent injection port and is connected to the reagent storage bottle; The second channel includes several metering branches of different volumes, which can be selected for use by different joint positions on the upper plate; The third channel is connected to the liquid injection connector and is used to output quantitative reagents to the downstream chip. The downstream of the second channel may be equipped with an overflow port connected to the waste liquid tank to discharge excess reagent in order to maintain measurement accuracy.

10. The liquid separation device according to claim 3, characterized in that, The power strip, temperature control structure, mixing chamber, and interface board are all encased in a shell and interconnected via conduits, endotracheal connectors, and positioning structures, forming a detachable modular assembly. The temperature control structure is located in the middle layer and provides reagent storage and temperature control functions. The power strip is located above it and completes the puncture operation through a closing action. The mixing chamber is located inside the temperature control structure and is directly connected to the reagent storage bottle. The interface board is located below it to form a liquid output path, thus constituting an upper and lower stacked automatic liquid dispensing system.

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