Multifunctional sample reaction plate preparation platform

Abstract

The invention relates to a multifunctional sample reaction plate preparation platform. The multifunctional sample reaction plate preparation platform comprises a sample transfer module, a reagent adding module, a drying module and a heat sealing module. The sample pipetting mechanism is detachably connected with the sample liquid sucking and spraying driving mechanism through the pipetting head quick-changing equipment; the reagent adding module comprises a reagent pipetting mechanism, a reagent pipetting needle and a reagent pipetting chamber which are communicated, the reagent pipetting chamber is provided with a reagent pipetting structure, and the reagent pipetting structure can provide pressure for the reagent pipetting chamber, so that a reaction reagent in the reagent pipetting chamber is sprayed out through the reagent pipetting needle; the drying module is used for drying a biological sample in a sample plate of the drying preparation station, and / or drying a biological sample in a reaction plate of the drying preparation station; and the heat sealing module is used for sealing the reaction plate at the heat sealing station. The flux is not limited, the plate making speed is high, sample evaporation loss and aerosol-mediated cross contamination are avoided, and meanwhile, the concentration of a reaction reagent is not easy to dilute.

Description

Technical Field

[0001] This application relates to the field of sample reaction plate preparation technology, and in particular to a multifunctional sample reaction plate preparation platform. Background Technology

[0002] In fields such as biological experiments and medical testing, the sample reaction plate preparation platform is the core equipment for reaction plate preparation. Currently, the throughput of existing sample reaction plate preparation equipment is limited. Most equipment has a fixed 96-channel design, which results in slow plate preparation speed and makes it difficult to meet the high-efficiency requirements of large-scale experiments.

[0003] Secondly, during the experiment, when polymerase chain reaction (PCR) amplification or enzyme-catalyzed oscillation are involved, the liquid in the reaction wells of the reaction plate is prone to vapor pressure changes due to temperature gradients, resulting in sample evaporation loss and aerosol-mediated cross-contamination.

[0004] Furthermore, the residual moisture in biological samples after transfer to the reaction plate directly dilutes the concentration of reagents within the reaction system. For small-scale plate preparation, this not only reduces the reaction rate and sufficiency, leading to a significant decrease in experimental efficiency, but may also cause reaction failure or deviations in experimental results due to insufficient concentration. Summary of the Invention

[0005] Based on this, a multifunctional sample reaction plate preparation platform is provided to address the problems of limited throughput, slow plate preparation speed, and difficulty in meeting the high-efficiency requirements of large-scale experiments. At the same time, it solves the problems of sample evaporation loss, aerosol-mediated cross-contamination, and easy dilution of reaction reagent concentration.

[0006] Embodiments of this application propose a multifunctional sample reaction plate preparation platform, comprising:

[0007] The sample transfer module includes a sample pipette tip module and a sample pipette arm. The sample pipette tip module includes a sample pipetting mechanism, a sample aspiration and dispensing drive mechanism, and a quick-change device for the pipette tip. The sample pipetting mechanism is detachably connected to the sample aspiration and dispensing drive mechanism via the quick-change device. The sample pipette arm is driven to the sample pipette tip module and is used to drive the sample pipette tip module to move to the sample transfer station to transfer the biological sample in the sample plate of the sample transfer station to the reaction plate of the sample transfer station.

[0008] A reagent adding module includes a reagent pipetting mechanism, which comprises a reagent pipetting holder, a reagent pipetting chamber, and a reagent pipetting needle. The reagent pipetting needle and the reagent pipetting chamber are connected. The reagent pipetting chamber is provided with a reagent pipetting structure that provides pressure to the reagent pipetting chamber, causing the reaction reagent inside the chamber to be ejected through the reagent pipetting needle. The reagent pipetting holder is driven to the reagent pipetting needle and is used to drive the reagent pipetting needle to move to the reagent adding station to add the reaction reagent to the reaction plate at the reagent adding station.

[0009] The drying module is provided with a drying preparation station located between the sample transfer station and the reagent addition station. The drying module is used to dry the biological samples in the sample plate of the drying preparation station and / or to dry the biological samples in the reaction plate of the drying preparation station.

[0010] The heat sealing module is equipped with a heat sealing station located downstream of the reagent addition station, which is used to seal the reaction plate of the heat sealing station.

[0011] In one embodiment, the heat-sealing module includes:

[0012] The traction mechanism includes a heat-sealing motion frame that slides horizontally and a heat-sealing clamping assembly disposed on the heat-sealing motion frame. The heat-sealing clamping assembly clamps the heat-sealing film, and the heat-sealing motion frame is used to drive the heat-sealing clamping assembly to pull the heat-sealing film to move a preset length in the horizontal direction.

[0013] The cutting mechanism includes a cutting linkage seat that slides horizontally and a cutting component disposed on the cutting linkage seat. The cutting component is used to cut a heat-sealing film of a preset length to obtain a heat-sealing film sheet.

[0014] The heat-sealing mechanism includes a stamping component and a heat-sealing adsorption component; the heat-sealing adsorption component is used to grip the heat-sealing film; the stamping component is used to heat-seal the cut heat-sealing film onto the reaction plate.

[0015] In one embodiment, the traction mechanism further includes a hook assembly slidably disposed between the heat-sealing motion frame and the cutting linkage seat; the hook assembly is used to pull the cutting assembly to the bottom end of the heat-sealing mechanism.

[0016] In one embodiment, the stamping assembly includes a stamping fixing plate, a stamping heating plate, and a stamping linkage plate arranged at intervals along a vertical direction; the stamping linkage plate is slidably disposed between the stamping fixing plate and the stamping heating plate; a plurality of first slide rods are mounted on the stamping linkage plate, and a first heat-sealing elastic element is fitted on the first slide rod; the first heat-sealing elastic element is sandwiched between the stamping fixing plate and the stamping linkage plate; a suction cup is provided at the bottom end of each of the plurality of first slide rods; the suction cup is used to grasp the heat-sealing film.

[0017] In one embodiment, the drying module includes:

[0018] A drying chamber is used for storing and drying consumables; a drying and loading mechanism includes a drying drive mechanism, wherein the drying drive mechanism includes a loading and loading sliding seat that is slidably arranged in the horizontal direction, and a loading and loading lifting part is provided on the side of the loading and loading sliding seat facing the drying chamber, the loading and loading lifting part is used to support the consumables, and the drying drive mechanism is used to drive the consumables to be loaded and loaded inside the drying chamber.

[0019] In one embodiment, the drying module includes:

[0020] A drying and conveying mechanism is provided, which moves the consumables through the drying preparation station; the drying and conveying mechanism is connected between the sample transfer station and the reagent addition station, and is used to transport the sprayed reaction plate to the drying preparation station and / or the reagent addition station.

[0021] In one embodiment, the drying module further includes a drying lifting mechanism, which includes a drying lifting drive, and the output end of the drying lifting drive is provided with a drying support seat.

[0022] The drying support is used to support the consumables, and the drying lifting drive is used to adjust the vertical height of the consumables so as to drive the consumables to detach from the drying transmission mechanism.

[0023] In one embodiment, the multifunctional sample reaction plate preparation platform further includes:

[0024] A stack device is used to store consumables and to interact with the consumables in the sample transfer module, the reagent addition module, and the heat sealing module; the consumables include the sample plate and the reaction plate.

[0025] In one embodiment, the stacking device is at least partially connected to the sample transfer module for interacting with the sample transfer station on the sample plate and the reaction plate, so as to provide the sample plate and the reaction plate to the sample transfer station.

[0026] And / or, the stacking device is at least partially connected to the reagent adding module for interacting with the reagent adding station to receive the reaction plate;

[0027] And / or, the stacking device is at least partially connected to the heat sealing station for interacting with the heat sealing station to receive the reaction plate.

[0028] In one embodiment, the stacking device includes:

[0029] A hierarchical stack module includes several independent storage spaces, configured to independently access the consumables within any of the independent storage spaces; the consumables include sample boards;

[0030] A stackable module, including stacked storage space, is configured to sequentially access consumables within the stacked storage space in a first-in-first-out (FIFO) order; the consumables include reaction plates.

[0031] According to an embodiment of the multifunctional sample reaction plate preparation platform of this application, the sample pipetting arm drives the sample pipetting head module to move automatically. The quick-change device for the pipetting head detachably connects the sample pipetting mechanism to the sample aspiration and dispensing drive mechanism, so that the sample pipetting mechanism can be separated from the sample aspiration and dispensing drive mechanism. The sample aspiration and dispensing drive mechanism can connect to sample pipetting mechanisms with different channels, such as 96 channels and 384 channels, which can adapt to the pipetting needs of different experimental scenarios such as high throughput and low throughput, without the need to configure a complete set of equipment separately for different throughputs; or sample pipetting mechanisms of different volumes, covering a variety of specifications such as 10ul, 30ul, 50ul, 200ul and 1000ul, which can meet the liquid transfer operations of micro-volume, regular volume and even large range, and adapt to the accuracy and range requirements of different experiments; or sample pipetting mechanisms of different specifications and types of consumables from different manufacturers. With the above setup, since the sample aspiration and dispensing drive mechanism is reusable, only different specifications of sample pipetting mechanisms need to be replaced to achieve the functions of different specifications of pipettes. There is no need to purchase a complete set of pipetting equipment separately for each pipetting need, which greatly reduces the overall procurement cost of the equipment. At the same time, in the maintenance process, the faulty sample pipetting mechanism can be disassembled, repaired or replaced separately without stopping the entire set of equipment for maintenance. Experimenters can quickly replace the corresponding specification of sample pipetting mechanism according to real-time experimental needs without re-adjusting the power system of the entire set of equipment. This allows for flexible switching of experimental scenarios, reduces maintenance costs and equipment downtime, and improves experimental efficiency.

[0032] Secondly, in the reagent addition module, by moving the reagent pipette above the reagent aspiration station, the reagent pipette needle and reagent pipetting chamber are moved to the reagent aspiration station. Since the reagent plate containing the reagent is placed at the reagent aspiration station, the reagent can be aspirated using the reagent pipette needle. Then, the reagent pipette holder moves the reagent pipette needle and reagent pipetting chamber to the reagent addition station, where pressure is applied to the reagent pipetting chamber to spray the reagent from the chamber through the reagent pipette needle into the reaction plate placed at the reagent addition station. Compared to existing pipetting modules, by applying pressure to the reagent pipetting chamber, the reagent can be ejected at high speed from the reagent pipette needle, improving the working efficiency of the reagent pipetting mechanism.

[0033] In addition, the drying module is adapted to the needs of small-scale plate preparation. After the sample is transferred to the reaction plate, the moisture is evaporated by the drying module, which can accurately control the volume of the reaction system and meet the stringent requirements of small-scale experiments on sample concentration and volume. At the same time, it can improve the accuracy of plate preparation and reagent utilization. There is no need to compensate for the effect of water dilution by increasing the initial concentration of reagents. While ensuring the accuracy of pipetting and reaction, it reduces the cost of using high-concentration reagents and avoids reagent waste.

[0034] Meanwhile, the heat-sealing module constructs a physical isolation layer on the surface of the reaction plate. Through thermo-adhesive bonding technology, a polymer sealing film, such as a polypropylene composite film, is formed with the edge of the plate to create an airtight seal, thereby achieving independent sealing of the reaction unit. This effectively prevents the migration of substances between the pores and avoids the liquid in the reaction pores of the reaction plate from easily causing vapor pressure changes due to temperature gradients, which could lead to sample evaporation loss and aerosol-mediated cross-contamination. It also prevents the concentration of the reaction reagents from being diluted. Attached Figure Description

[0035] Figure 1 This is one of the schematic diagrams of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0036] Figure 2 This is a schematic diagram of the sample transfer module according to an embodiment of this application.

[0037] Figure 3 This is a schematic diagram of the logistics transmission interaction module in the sample transfer module of an embodiment of this application.

[0038] Figure 4 This is a schematic diagram of the structure of the logistics transmission interaction component in the sample transfer module of an embodiment of this application.

[0039] Figure 5 This is another structural schematic diagram of the logistics transmission interaction component in the sample transfer module of an embodiment of this application.

[0040] Figure 6This is a schematic diagram of the transmission interaction limiting component in the sample transfer module of an embodiment of this application.

[0041] Figure 7 This is a schematic diagram of the docking of two logistics transmission interaction components in the sample transfer module of an embodiment of this application.

[0042] Figure 8 The overall three-dimensional structure of the drying module in the sample transfer module of one embodiment of this application. Figure 2 .

[0043] Figure 9 for Figure 8 Enlarged view of the structure at point A in the middle.

[0044] Figure 10 This is a three-dimensional structural diagram of the drying chamber of the drying module in the sample transfer module of an embodiment of this application.

[0045] Figure 11 This is a cross-sectional view of the drying chamber of the drying module in the sample transfer module of an embodiment of this application.

[0046] Figure 12 This is a three-dimensional structural diagram of the drying and loading mechanism of the drying module in the sample transfer module of an embodiment of this application.

[0047] Figure 13 for Figure 12 Enlarged view of the structure at point B in the middle.

[0048] Figure 14 This is a partial three-dimensional structural diagram of the drying and conveying mechanism of the drying module in the sample transfer module of an embodiment of this application.

[0049] Figure 15 This is a schematic diagram of the layered stacked module of the drying module in the sample transfer module of an embodiment of this application.

[0050] Figure 16 This is a schematic diagram of the stack transport unit of the layered stack module in the sample transfer module of an embodiment of this application.

[0051] Figure 17 This is a schematic diagram of the structure of the layered rotation component in the sample transfer module of an embodiment of this application.

[0052] Figure 18 This is a schematic diagram of the structure of the layered telescopic pick-and-place component in the sample transfer module of an embodiment of this application.

[0053] Figure 19 This is a schematic diagram of the stacked module in the sample transfer module of an embodiment of this application.

[0054] Figure 20This is a partial cross-sectional view of the stacked module in the sample transfer module of an embodiment of this application.

[0055] Figure 21 This is a schematic diagram of the stacking transfer unit of the stacked transfer module in the sample transfer module of an embodiment of this application.

[0056] Figure 22 This is a partial structural diagram illustrating the stack docking hole in the sample transfer module of an embodiment of this application.

[0057] Figure 23 This is a schematic diagram of the stacking and lifting unit of the stacked stack module in the sample transfer module of an embodiment of this application.

[0058] Figure 24 This is a schematic diagram of the overall structure of a sample transfer module according to an embodiment of this application. Figure 1 .

[0059] Figure 25 This is a schematic diagram of the overall structure of a sample transfer module according to an embodiment of this application. Figure 2 .

[0060] Figure 26 This is a top view of the sample transfer module according to an embodiment of this application.

[0061] Figure 27 This is a schematic diagram of the sample aspiration and dispensing mechanism in a sample pipette module according to an embodiment of this application.

[0062] Figure 28 This is a schematic diagram of the structure of a sample pipette module according to an embodiment of this application.

[0063] Figure 29 This is a cross-sectional view showing the piston shaft assembly structure in a sample pipette module according to an embodiment of this application.

[0064] Figure 30 This is a cross-sectional view of the sample pipetting mechanism in a sample pipetting head module according to an embodiment of this application.

[0065] Figure 31 for Figure 30 A magnified view of a portion of point C.

[0066] Figure 32 This is an exploded view of the piston shaft assembly in a sample pipette module according to an embodiment of this application.

[0067] Figure 33 This is a schematic diagram of the structure of the sample pipette head module after the head retraction mechanism has been reset according to an embodiment of this application.

[0068] Figure 34This is a schematic diagram of the retraction mechanism in the sample pipette module according to an embodiment of this application during the retraction process.

[0069] Figure 35 This is a schematic diagram of the sample pipette tip support assembly in the sample transfer module according to an embodiment of this application.

[0070] Figure 36 This is a schematic diagram of the structure of a quick-change pipette tip device according to an embodiment of this application.

[0071] Figure 37 This is a partial structural schematic diagram of a quick-change pipette tip device according to an embodiment of this application.

[0072] Figure 38 This is a front view of a quick-change pipette tip device according to an embodiment of this application.

[0073] Figure 39 for Figure 38 Sectional view at point PP.

[0074] Figure 40 This is a schematic diagram of the sample pipetting mechanism in a pipetting device according to an embodiment of this application.

[0075] Figure 41 This is a schematic diagram of the structure of a sample pipetting device cleaning apparatus according to an embodiment of this application.

[0076] Figure 42 This is a cross-sectional view of a sample pipetting apparatus cleaning device according to an embodiment of this application.

[0077] Figure 43 This is an exploded view of the sample pipetting apparatus cleaning device after it has been flipped over, according to an embodiment of this application.

[0078] Figure 44 This is a cross-sectional view of a sample transfer and cleaning channel in a sample pipetting apparatus cleaning device according to an embodiment of this application.

[0079] Figure 45 This is a schematic diagram of the automatic water inlet control mechanism in the sample pipetting apparatus cleaning device according to an embodiment of this application.

[0080] Figure 46 This is a schematic diagram illustrating the structure of the sample transfer module in an embodiment of this application, showing the sample pipetting frame module.

[0081] Figure 47 This is a schematic diagram illustrating the structure of the outer tube assembly in the sample transfer module of an embodiment of this application.

[0082] Figure 48 This is a schematic diagram of the front door structure in a sample transfer module according to an embodiment of this application.

[0083] Figure 49This is a schematic diagram of the sample pipette arm in a sample transfer module according to an embodiment of this application.

[0084] Figure 50 This is a schematic diagram of the Z-axis drive assembly of the sample pipette arm in a sample transfer module according to an embodiment of this application.

[0085] Figure 51 This is a cross-sectional view of the Z-axis drive assembly of the sample pipette arm in a sample transfer module according to an embodiment of this application.

[0086] Figure 52 This is a schematic diagram of the sample plate conveying mechanism of the logistics transmission interaction module in the sample transfer module of an embodiment of this application.

[0087] Figure 53 for Figure 52 A cross-sectional view at point QQ.

[0088] Figure 54 This is a schematic diagram of the structure of the local board grasping component of the logistics transmission interaction module in the sample transfer module of an embodiment of this application.

[0089] Figure 55 This is a schematic diagram of the reaction plate conveying mechanism of the logistics transmission interaction module in the sample transfer module of an embodiment of this application.

[0090] Figure 56 This is a schematic diagram of the scanning module in the sample transfer module of an embodiment of this application.

[0091] Figure 57 This is a schematic diagram of the reagent addition module provided in one embodiment of this application.

[0092] Figure 58 This is another schematic diagram of the reagent adding module provided in an embodiment of this application.

[0093] Figure 59 This is a schematic diagram of the reagent addition rack of a reagent addition module provided in an embodiment of this application.

[0094] Figure 60 This is a schematic diagram of the reagent pipetting mechanism of a reagent adding module provided in an embodiment of this application.

[0095] Figure 61 This is a schematic diagram of the reagent addition driving mechanism of a reagent addition module provided in an embodiment of this application.

[0096] Figure 62 This is a cross-sectional view of the cooling mechanism of a reagent adding module provided in an embodiment of this application.

[0097] Figure 63 for Figure 62A magnified view of a section at point D.

[0098] Figure 64 This is a schematic diagram of the insulation component of a reagent adding module provided in an embodiment of this application.

[0099] Figure 65 This is a schematic diagram of the structure of the heat-conducting component of the reagent adding module provided in an embodiment of this application.

[0100] Figure 66 This is a schematic diagram of the structure of the circulation component of the reagent addition module provided in one embodiment of this application.

[0101] Figure 67 This is a three-dimensional structural diagram of a heat-sealing module according to an embodiment of this application.

[0102] Figure 68 The internal three-dimensional structure of a heat-sealing module according to an embodiment of this application. Figure 1 .

[0103] Figure 69 The internal three-dimensional structure of a heat-sealing module according to an embodiment of this application. Figure 2 .

[0104] Figure 70 The three-dimensional structure of the traction mechanism and the cutting mechanism in the heat sealing module of one embodiment of this application. Figure 1 .

[0105] Figure 71 The three-dimensional structure of the traction mechanism and the cutting mechanism in the heat sealing module of one embodiment of this application. Figure 2 .

[0106] Figure 72 This is a partial cross-sectional view of the traction mechanism in a heat-sealing module according to an embodiment of this application.

[0107] Figure 73 This is a three-dimensional structural diagram of the hook component in the heat-sealing module according to an embodiment of this application.

[0108] Figure 74 This is a three-dimensional structural diagram of the cutting mechanism in the heat-sealing module according to an embodiment of this application.

[0109] Figure 75 This is a three-dimensional structural diagram of the heat sealing mechanism in a heat sealing module according to an embodiment of this application.

[0110] Figure 76 This application presents an embodiment of the overall three-dimensional structure of the material guiding mechanism in the heat sealing module. Figure 1 .

[0111] Figure 77 This application presents an embodiment of the overall three-dimensional structure of the material guiding mechanism in the heat sealing module. Figure 2 .

[0112] Figure 78 This is a three-dimensional structural diagram of the heat sealing conveying mechanism in a heat sealing module according to an embodiment of this application.

[0113] Figure 79 This is a second schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0114] Figure 80 This is the third schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0115] Figure 81 This is the fourth schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0116] Figure 82 This is the fifth schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0117] Figure 83 This is a schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0118] Figure 84 This is the seventh schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0119] Figure 85 This is the eighth schematic diagram of a multifunctional sample reaction plate preparation platform according to an embodiment of this application.

[0120] Figure label:

[0121] 0001, Sample plate; 0002, Reaction plate;

[0122] 10000, Sample Transfer Module;

[0123] 1000, Sample pipetting tip module;

[0124] 1100. Sample pipetting mechanism;

[0125] 1110 Piston chamber assembly; 1111 Piston chamber; 1111a First quick-change part; 11111 First fixing plate; 11111a Second receiving groove; 11112 Second fixing plate; 1112 Pipe tip connection part; 11120 Sample pipette tip; 11121 First receiving groove; 11122 Second sealing ring;

[0126] 1120 Piston shaft assembly; 1121 Piston shaft pressure plate; 1121a Second quick-change part; 1122 Piston shaft; 11221 Rod part; 11222 Fixing part; 1123 Flexible fixing assembly; 11231 Piston shaft fixing plate; 11231a First fixing groove; 11231b Second fixing groove; 11232 Flexible component;

[0127] 1130. Sealing assembly;

[0128] 1200. Sample aspiration and spraying mechanism;

[0129] 1300. Quick-change pipette tip device;

[0130] 1310. Positioning module; 1301. Cavity positioning submodule; 1302. Shaft positioning submodule; 1311. Positioning groove; 13111. Positioning groove; 13112. Positioning inlet; 13113. Clamping port; 13114. First spring seat; 1312. Positioning baffle; 13121. Second spring seat;

[0131] 1320. Quick-change module; 1321. Clamping mechanism; 13211. Clamping block; 13211a. Clamping surface; 1322. Quick-change drive mechanism; 13221. Moving cam block; 13221a. Cam inclined surface; 13222. Linear drive component; 132221. Drive plate; 132222. Locking shaft; 132222a. Drive end; 132222b. Operating part; 13223. Base plate; 132231. Drive slide groove;

[0132] 1330. Quick-change reset mechanism; 1331. First quick-change elastic reset component; 1332. Second quick-change elastic reset component;

[0133] 1400 Head retraction mechanism; 1410 Head retraction plate; 1420 Head retraction drive component; 1421 Head retraction rod; 14211 Protrusion; 1422 Head retraction reset component;

[0134] 1500, Specification identification module; 1510, Identification element; 1520, Sensing element;

[0135] 1600. Interface sample pipetting side plate; 1610. Interface connection plate; 1620. Interface mounting plate;

[0136] 1700, First guide mechanism; 1710, First guide bearing; 1720, First guide shaft;

[0137] 2000, Sample pipetting device cleaning equipment;

[0138] 2010, Tank structure; 2011, Water inlet chamber; 2011a, Second inclined surface structure; 20111, Single water inlet chamber; 20112, Cross partition; 2012, Drainage chamber; 2012a, First inclined surface structure; 2013, Cleaning tank; 2014, Mounting base plate; 20141, Sample cleaning sealing strip; 2015, Cleaning base;

[0139] 2020, Channel mechanism; 2021, Sample transfer and cleaning channel;

[0140] 2030. Water inlet mechanism; 2031. Water inlet connector;

[0141] 2040, First drainage mechanism;

[0142] 2050, Second drainage mechanism;

[0143] 2060. Overflow monitoring agencies;

[0144] 2070. Ultrasonic cleaning transducer;

[0145] 2080 Automatic water inlet control mechanism; 2081 Liquid storage container; 20811 Water inlet; 20812 Water outlet; 20813 Vent; 2082 Water replenishment component; 20821 Water inlet pipe; 20822 Pressure reducing valve; 20823 Automatic water inlet valve; 2083 Water delivery component; 2084 Liquid level monitoring component;

[0146] 3000, Sample pipette;

[0147] 3100, Y-axis drive assembly; 3110, Y-axis displacement unit; 3111, guide rail mounting plate; 3112, first guide rail; 3120, Y-axis drive unit; 3121, Y-axis motor; 3122, Y-axis synchronous pulley; 3123, Y-axis synchronous belt; 3124, synchronous belt connecting plate; 3125, cable connecting plate; 3130, cable chain assembly;

[0148] 3200, Z-axis drive assembly; 3210, Z-axis displacement unit; 3211, Z-axis mounting plate; 3212, second guide shaft; 3212a, guide shaft fixing plate; 3213, second guide bearing; 3214, vertical plate; 3220, Z-axis drive unit; 3221, nut and screw transmission element; 3222, Z-axis synchronous pulley; 3223, Z-axis synchronous belt; 3224, Z-axis motor;

[0149] 4000, Sample pipette tip support assembly; 4100, Sample pipette tip base plate; 4200, Sample pipette tip support plate; 4300, Sample pipette tip holder;

[0150] 5000, Sample pipetting frame module; 5100, Machining beam; 5110, Casters; 5120, Tabletop; 5200, Sample pipetting crossbeam; 5300, Sample pipetting upright beam; 5400, Appearance components; 5410, Sample pipetting side panel; 5420, Sample pipetting rear panel; 5430, Sample pipetting top panel; 5440, Sample pipetting front panel; 5441, Front door; 5441a, Constant load spring; 5441b, Lock; 5441c, Breathing light; 5441d, Sample pipetting sub-panel; 5500, Lighting assembly;

[0151] 6000, Logistics Transmission Interaction Module;

[0152] 6011, Transmission interaction frame; 60111, Transmission interaction drive wheel; 60112, Transmission interaction driven wheel; 60113, Transmission interaction tension wheel;

[0153] 6012. Transmission and interaction components;

[0154] 6015. Transmission and interaction support components;

[0155] 6016, Logistics docking structure; 60161, Logistics docking column; 60162, Logistics docking hole; 6017, Transmission interaction fixing seat; 6018, Transmission interaction guide; 6019, Transmission interaction drive shaft;

[0156] 6020. Transmission interaction limiting component; 6021. First limiting clamping component; 6022. Second limiting clamping component; 6023. Transmission interaction mounting plate; 6024. First transmission interaction driver; 6025. Second transmission interaction driver;

[0157] 6030, First transmission interaction detection component;

[0158] 6040, First transmission interaction sensor; 50, Second transmission interaction sensor; 60, Transmission interaction contact element;

[0159] 6100, Sample plate conveying mechanism;

[0160] 6110, Sample plate positioning assembly; 6111, Positioning side plate; 6111a, First coarse positioning ramp; 6112, Elastic side plate; 6112a, Second coarse positioning ramp; 6113, Positioning elastic element; 6113a, Positioning bead; 6113b, Positioning spring;

[0161] 6120. Sample board gripping assembly; 6121. Gripping drive component; 6121a. Gripping base plate; 6121b. Gripping motor; 6121c. Gripping synchronous pulley; 6121d. Gripping synchronous belt; 6121e. Gripping slide rail; 6122. Gripper; 6122a. First gripping plate; 6122b. Second gripping plate;

[0162] 6200, Reaction plate conveying mechanism;

[0163] 6210. Reaction plate positioning assembly; 6211. Reaction plate positioning component;

[0164] 6220. Reaction plate shaping assembly; 6221. Clamping plate; 6222. Clamping drive component;

[0165] 6230, Reaction Plate Conveyor Belt;

[0166] 6300. Drying and conveying mechanism; 6310. Sub-conveying unit;

[0167] 7000, barcode scanning module; 7100, fixed barcode reader; 7200, mounting bracket; 7300, scanner connector;

[0168] 8000, Stacking device;

[0169] 8100, layered stacked modules;

[0170] 8110, Tiered storage unit; 8111, Independent storage space;

[0171] 8120, Location Identification Unit;

[0172] 8130, Stacking and handling unit;

[0173] 8131, Layered conveying unit; 81312, Layered lifting motor; 81313, Lifting synchronous pulley; 81314, Lifting synchronous belt; 81315, Lifting mounting plate; 81316, Lifting guide rail; 81317, Lifting frame; 81318, Counterweight mechanism; 81319, Pulley structure; 81310, Steel wire rope; 81310, Counterweight block;

[0174] 8132, Layered rotating assembly; 81321, Rotary motor; 81322, Rotary drive gear; 81323, Rotary driven gear;

[0175] 8133, Layered telescopic pick-and-place assembly; 81331, Pick-and-place rack; 81332, Pick-and-place tray; 81333, Pick-and-place motor; 81334, Pick-and-place guide rail; 81335, In-situ detection unit;

[0176] 8140, Layered Framework;

[0177] 8200, stacked module;

[0178] 8210, Stacked storage unit; 8211, Stacked cage; 82111, Stacked storage space; 8212, Snap-on mechanism; 82121, Snap-on component; 82122, Snap-on drive assembly; 82122a, Electromagnetic drive component; 82122b, Snap-on reset component; 82122c, Snap-on connecting rod;

[0179] 8220, Stacking and Transfer Unit;

[0180] 8221. Transfer frame; 82211. Stacking base; 8222. Transfer component; 8223. Transfer drive wheel; 8224. Transfer driven wheel; 8225. Transfer motor;

[0181] 8226. Diffuse reflection sensor;

[0182] 8227. Stack docking post;

[0183] 8228, Stack docking hole;

[0184] 8230, Stacking and Lifting Unit;

[0185] 8231, Stacking and lifting pallets; 82311, First guard edge; 82312, Second guard edge;

[0186] 8232. Stacking and lifting drive component;

[0187] 8233, Stacking and Lifting Guide Component;

[0188] 8240, Stacked Frames;

[0189] 20000, Reagent Addition Module;

[0190] 20010. Reagent dispensing rack; 20011. Reagent dispensing station; 20012. Reagent aspiration station; 20013. Reagent cleaning container placement area; 20014. Reagent dispensing frame; 20015. Reagent dispensing base plate; 20016. Reagent dispensing interlayer plate; 20017. Reagent dispensing longitudinal beam; 20018. Reagent dispensing top plate; 20019. Reagent dispensing side plate;

[0191] 20020, Reagent pipetting mechanism; 20021, Reagent pipetting rack; 20022, Reagent pipetting chamber; 200221, Reagent pipetting air inlet; 20023, Reagent pipetting needle; 20024, First valve; 20025, Reagent pipetting shaft; 20026, Reagent pipetting drive component; 20027, Second valve;

[0192] 20030, Matcher;

[0193] 20040, Reagent adding drive mechanism; 20041, Reagent crossbeam; 20042, First reagent adding drive component; 20043, Second reagent adding drive component; 20044, Third reagent adding drive component;

[0194] 20051. Reagent addition cleaning components; 20052. Reagent addition cleaning pneumatic components;

[0195] 20060, Refrigeration mechanism; 20061, Insulation component; 200611, Insulation module; 200612, Adapter; 200613, Insulation board; 200614, Insulation shell; 200615, Refrigeration pressure plate; 200616, Condensate tank; 20062, Thermal conductive component; 200621, Reagent adding radiator; 200622, Reagent adding cooling fan; 20063, Circulation component; 200631, Circulation pump; 200632, Liquid storage tank;

[0196] 30000, heat-sealed module;

[0197] 30100, Material guiding mechanism;

[0198] 30110, Material guide frame; 30111, Adjustment assembly;

[0199] 30120, Heat-sealed drive wheel mechanism; 30121, Suspension; 30122, Lifting frame; 30123, Heat-sealed drive wheel assembly; 30124, Second slide bar; 30125, Fourth heat-sealed elastic element;

[0200] 30130, Heat-sealed driven wheel assembly;

[0201] 30200, Traction mechanism;

[0202] 30210, Heat-sealed motion frame; 30211, Heat-sealed cam; 30212, Heat-sealed linkage; 30213, Linkage slide groove;

[0203] 30220, Heat-sealing clamping assembly; 30221, Heat-sealing clamping part; 30222, First magnetic plate; 30223, Heat-sealing clamping plate; 30224, Second magnetic plate;

[0204] 30230, Hook handle assembly; 30231, Heat-sealed linkage rod;

[0205] 30232, Swing component; 302321, Swing hook; 302322, First heat-sealed inclined surface; 302323, Second heat-sealed inclined surface;

[0206] 30233, heat-sealed guide roller;

[0207] 30240, First heat-sealing drive component; 30250, First heat-sealing connector;

[0208] 30300, Cutting mechanism;

[0209] 30310, Cutting linkage seat; 30311, Second heat-sealing elastic element; 30312, Cutting protrusion;

[0210] 30320, Cutting assembly; 30321, Cutting frame; 30322, Cutting piece; 30323, Third heat-sealing elastic element;

[0211] 30400, heat sealing mechanism;

[0212] 30410, stamping assembly; 30411, stamping fixing plate; 30412, stamping heating plate; 30413, stamping linkage plate; 30414, second heat sealing drive component;

[0213] 30420, Heat-sealing adsorption assembly; 30421, First slide bar; 30422, First heat-sealing elastic element; 30423, Suction cup;

[0214] 30500, heat sealing conveyor mechanism;

[0215] 30510, Heat sealing conveyor section; 30520, Heat sealing conveyor mounting base; 30530, Third heat sealing drive component; 30540, Second heat sealing connector;

[0216] 40000, drying module;

[0217] 40100, Drying oven; 40110, Notch;

[0218] 40120. Drying adjustment mechanism; 40121. Rewinding component; 40122. Screening component; 40123. Loading / unloading port;

[0219] 40130, Insulation layer;

[0220] 40140. Drying installation parts; 40141. Slide groove;

[0221] 40150 Temperature sensing element; 40160 Heating element; 40170 Blowing element;

[0222] 40200 Drying and loading mechanism; 40210 Drying support component; 40220 Drying slide bar; 40230 Drying counterweight base;

[0223] 40240, Horizontal drying drive unit; 40241, Drying base; 40242, Drying track; 40243, Pick-up and drop sliding seat; 40244, Pick-up and drop lifting part; 40245, First rotating shaft; 40246, Second rotating shaft; 40247, Drying conveyor belt;

[0224] 40250, Drying synchronous belt;

[0225] 40400, Drying lifting mechanism; 40410, Drying lifting drive component; 40420, Drying support seat. Detailed Implementation

[0226] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0227] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0228] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0229] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0230] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0231] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0232] See Figure 1 , Figure 24 as well as Figure 57 , Figure 58At least one embodiment of this application proposes a multifunctional sample reaction plate preparation platform, which includes a sample transfer module 10000 and a reagent addition module 20000. The sample transfer module 10000 includes a sample pipette head module 1000 and a sample pipette arm 3000. The sample pipette head module 1000 includes a sample pipetting mechanism 1100, a sample aspiration and dispensing driving mechanism 1200, and a pipette head quick-change device 1300. The sample pipetting mechanism 1100 is detachably connected to the sample aspiration and dispensing driving mechanism 1200 via the pipette head quick-change device 1300. The sample pipette arm 3000 is drivenly connected to the sample pipette head module 1000 and is used to drive the sample pipette head module 1000 to move to the sample transfer station to transfer the biological sample in the sample plate 0001 of the sample transfer station to the reaction plate 0002 of the sample transfer station. The reagent adding module 20000 includes a reagent pipetting mechanism 20020, which includes a reagent pipetting holder 20021, a reagent pipetting chamber 20022, and a reagent pipetting needle 20023. The reagent pipetting needle 20023 and the reagent pipetting chamber 20022 are connected. The reagent pipetting chamber 20022 is provided with a reagent pipetting structure, which can provide pressure to the reagent pipetting chamber 20022 so that the reaction reagent in the reagent pipetting chamber 20022 is ejected through the reagent pipetting needle 20023. The reagent pipetting holder 20021 is driven to the reagent pipetting needle 20023 to move the reagent pipetting needle 20023 to the reagent adding station 20011 to add the reaction reagent to the reaction plate 0002 of the reagent adding station 20011.

[0233] According to the multifunctional sample reaction plate preparation platform of this application embodiment, the sample pipetting arm 3000 drives the sample pipetting head module 1000 to move automatically. The pipetting head quick-change device 1300 detachably connects the sample pipetting mechanism 1100 to the sample aspiration and dispensing liquid driving mechanism 1200, so that the sample pipetting mechanism 1100 can be separated from the sample aspiration and dispensing liquid driving mechanism 1200. The sample aspiration and dispensing liquid driving mechanism 1200 can connect to sample pipetting mechanisms 1100 with different channels, such as 96 channels and 384 channels, which can adapt to the pipetting needs of different experimental scenarios such as high throughput and low throughput, without the need to configure a complete set of equipment separately for different throughputs; or sample pipetting mechanisms 1100 with different volumes, covering multiple specifications such as 10ul, 30ul, 50ul, 200ul and 1000ul, which can meet the liquid transfer operations of micro-volume, regular volume and even large range, and adapt to the accuracy and range requirements of different experiments; or sample pipetting mechanisms 1100 with different specifications and different types of consumables from different manufacturers. With the above setup, since the sample aspiration and dispensing drive mechanism 1200 is reusable, only the sample pipetting mechanism 1100 of different specifications needs to be replaced to achieve the functions of different specifications of pipettes. There is no need to purchase a complete set of pipetting equipment separately for each pipetting need, which greatly reduces the overall procurement cost of the equipment. At the same time, in the maintenance process, the faulty sample pipetting mechanism 1100 can be disassembled, repaired or replaced separately without stopping the entire set of equipment for maintenance. Experimenters can quickly replace the sample pipetting mechanism 1100 of the corresponding specification according to the real-time experimental needs without re-adjusting the power system of the entire set of equipment. This allows for flexible switching of experimental scenarios, reduces maintenance costs and equipment downtime, and improves experimental efficiency.

[0234] Furthermore, in the reagent addition module 20000, by moving the reagent pipette 20021 above the reagent aspiration station 20012, the reagent pipette 20023 and the reagent pipetting chamber 20022 are moved to the reagent aspiration station 20012. Since the reagent plate containing the reagent is placed at the reagent aspiration station 20012, the reagent can be aspirated by the reagent pipette 20023. Then, the reagent pipette 20021 moves the reagent pipette 20023 and the reagent pipetting chamber 20022 to the reagent addition station 20011. The reagent pipetting structure then provides pressure to the reagent pipetting chamber 20022, allowing the reagent in the reagent pipetting chamber 20022 to be sprayed through the reagent pipette 20023 into the reaction plate 0002 placed at the reagent addition station 20011. Compared to existing pipetting modules, by providing pressure to the reagent pipetting chamber 20022, the reagent can be ejected at high speed from the reagent pipetting needle 20023, thus improving the working efficiency of the reagent pipetting mechanism 20020.

[0235] See Figure 1In some embodiments, the multifunctional sample reaction plate preparation platform further includes a drying module 40000, which is provided with a drying preparation station. The drying module 40000 is used to dry the biological samples in the sample plate 0001 in the drying preparation station, and / or to dry the biological samples in the reaction plate 0002 in the drying preparation station.

[0236] In some embodiments, the drying preparation station is located between the sample transfer station and the reagent addition station 20011.

[0237] According to the multifunctional sample reaction plate preparation platform of this application embodiment, the drying module 40000 is adapted to the needs of small system plate preparation. After the sample is transferred to the reaction plate 0002, the moisture is evaporated by the drying module 40000, which can accurately control the volume of the reaction system and meet the stringent requirements of small system experiments on sample concentration and volume. At the same time, it can improve the plate preparation accuracy and reagent utilization rate, without the need to increase the initial concentration of reagents to compensate for the effect of water dilution. Under the premise of ensuring the accuracy of pipetting and reaction, it reduces the cost of using high-concentration reagents and avoids reagent waste.

[0238] See Figure 1 In some embodiments, the multifunctional sample reaction plate preparation platform further includes a heat sealing module 30000, which is provided with a heat sealing station for sealing the reaction plate 0002 at the heat sealing station.

[0239] In some embodiments, the heat sealing station is located downstream of the reagent addition station 20011.

[0240] According to the multifunctional sample reaction plate preparation platform of this application embodiment, the heat sealing module 30000 constructs a physical isolation layer on the surface of the reaction plate 0002. Through thermosetting adhesive technology, a polymer sealing film, such as a polypropylene composite film, is formed with the edge of the plate to form an airtight seal, thereby achieving independent sealing of the reaction unit, effectively blocking the migration of substances between the holes, avoiding the vapor pressure change of the liquid in the reaction hole of the reaction plate 0002 due to the temperature gradient, which would lead to sample evaporation loss and aerosol-mediated cross-contamination, while also preventing the concentration of the reaction reagent from being diluted.

[0241] Understandably, reagent plates are used to store reaction reagents, which can be nucleic acid extraction reagents, PCR (Polymerase Chain Reaction) amplification reagents, immunoassay reagents, nucleic acid hybridization reagents, sample pretreatment reagents (such as lysis buffers and anticoagulants), chromogenic reagents, or enzyme-catalyzed reaction reagents, etc. In practice, reagent plates can be implemented using multi-well plates, with multiple independent reagent wells, each well used to store a specific amount of reaction reagent. Optionally, different wells on the same reagent plate can be pre-filled with the same or different reaction reagents. When different reaction reagents are pre-filled, they can be distinguished by the position (e.g., coordinates) of the well containing the corresponding reaction reagent on the reagent plate. Sample plate 0001 is used to store biological samples, which can be blood, serum, plasma, saliva, urine, tissue homogenate, cell suspension, cerebrospinal fluid, sputum, or fecal extracts, etc. Similar to the reagent plate, the sample plate 0001 can also be implemented using a multi-well plate. The sample plate 0001 has multiple independent sample wells, each used to store a specific amount of biological sample. Optionally, different biological samples can be stored in different sample wells on the same sample plate 0001, distinguished by their positions on the plate. The reaction plate 0002 provides a reaction site for the biological samples and reaction reagents. In practice, the reaction plate 0002 can also be implemented using a multi-well plate, with multiple independent reaction wells, each used to provide a reaction site for the biological samples and reaction reagents.

[0242] See Figure 1 The sample pipetting mechanism 1100 is provided with a pipette tip connection part 1112, and the sample aspiration and dispensing driving mechanism 1200 drives the sample pipetting mechanism 1100 to provide positive or negative pressure to the pipette tip connection part 1112.

[0243] See Figure 1 In some embodiments, the sample transfer module 10000 further includes a sample pipetting device cleaning device 2000, which includes a water inlet mechanism 2030, a first drainage mechanism 2040, and a plurality of independent sample transfer cleaning channels 2021. The water inlet mechanism 2030 is connected to the sample transfer cleaning channel 2021 to input cleaning solution, and the first drainage mechanism 2040 is connected to the sample transfer cleaning channel 2021 to discharge cleaning solution. Each sample transfer cleaning channel 2021 allows at least one sample pipetting tip 11120 to extend into it.

[0244] With the above setup, the sample pipetting device cleaning equipment 2000 can clean the sample pipetting tips 11120. During the cleaning process, the water inlet mechanism 2030 delivers cleaning solution to the sample transfer cleaning channel 2021. The cleaning solution cleans the items to be cleaned within the sample transfer cleaning channel 2021, and the cleaning solution within the sample transfer cleaning channel 2021 is discharged through the first drainage mechanism 2040. When the items to be cleaned are sample pipetting tips 11120 of the pipetting device, the sample aspiration and spraying liquid driving mechanism 1200 drives the sample pipetting tips 11120 to move, so that the sample pipetting tips 11120 are inserted one by one into the corresponding independent sample transfer cleaning channels 2021. By simulating the repeated aspiration and spraying of cleaning solution through the sample pipetting tips 11120, the cleaning solution fully contacts the inner and outer walls of the sample pipetting tips 11120, removing residual liquid, impurities, or contaminants from the sample pipetting tips 11120, thus achieving the cleaning effect. Since the sample transfer and cleaning channels 2021 corresponding to each sample pipette tip 11120 are independent, the problem of cross-contamination between sample pipette tips 11120 can be avoided, ensuring the cleanliness of the cleaning and improving the accuracy of experimental or test results.

[0245] See Figure 1 In some embodiments, the multifunctional sample reaction plate preparation platform further includes a material transport interaction module 6000, which is used to transport reagent plates, sample plates and reaction plates back and forth between the functional modules according to a preset path.

[0246] In practical implementation, the logistics transmission interaction module 600 can be implemented using a conveyor belt structure, a robotic arm, or other structures. For example, the logistics transmission interaction module 600 can transport reagent plates, sample plates, and reaction plates to various functional modules via a conveyor belt, or it can use a robotic arm to pick up the reagent plates, sample plates, and reaction plates and place them at the respective functional modules. Each functional module can process the reagent plates, sample plates, and reaction plates according to the corresponding operational steps in the reaction plate preparation process. The operational steps in the reaction plate preparation process may include, but are not limited to, sample transfer, sample drying, reagent addition, and heat sealing. When the functional modules are arranged along a preset path, to improve the efficiency of reaction plate preparation, each functional module can be arranged according to the reaction plate preparation process in a way that optimizes the transfer path of the reaction plates between the functional modules, so as to carry out reaction plate preparation in an orderly manner. Optionally, the functional modules can also be arranged according to the specific layout and size of each functional module to orderly realize the reaction plate preparation process.

[0247] In some embodiments, the logistics transmission interaction module 6000 includes a transmission interaction frame 6011 and a transmission interaction component 6012. The transmission interaction component 6012 is used to carry consumables and is movably disposed on the transmission interaction frame 6011.

[0248] With the above configuration, in the logistics transmission and interaction module 6000, the transmission and interaction frame 6011 provides positioning support for pipetting operations, and the transmission and interaction component 6012 can directly carry the sample plate 0001 and reaction plate 0002 to move between preset working positions, reducing intermediate links in the exchange and transfer of consumables and improving the interaction speed between each working position. Through the movement of the transmission and interaction component 6012, in coordination with the sample pipetting arm 3000 and sample pipetting head module 1000, the disconnect between consumable transfer and pipetting operations is avoided, ensuring plate preparation efficiency.

[0249] Combination Figures 3 to 5 As shown, in some embodiments, the transmission interaction component 6012 extends along a first direction and is movably disposed on the transmission interaction frame 6011 along the first direction; the logistics transmission interaction module 6000 includes a plurality of logistics transmission interaction components, which are arranged sequentially along the first direction, and a logistics docking structure 6016 is provided between two adjacent logistics transmission interaction modules 6000, and the two adjacent logistics transmission interaction modules 6000 are connected through the logistics docking structure 6016.

[0250] In some embodiments, the logistics transmission interaction module 6000 further includes a transmission interaction limiting component 6020, which includes a limiting member movably disposed on the transmission interaction frame 6011 and has an initial state and a limiting state. When the limiting member is in the initial state, the limiting member avoids the consumable, and the transmission interaction component 6012 drives the consumable to move past a preset working position. When the limiting member is in the limiting state, the limiting member and the consumable are limited and engaged at the preset working position, and the limiting member and the consumable are relatively stationary.

[0251] With the above setup, consumables are placed on the transmission interaction component 6012 of the logistics transmission interaction component. The transmission interaction component 6012 carries the consumables, and by moving the transmission interaction component 6012 along the first direction X, it can drive the consumables to move along the first direction X. The consumables enter the logistics transmission interaction component from one end, are carried by the transmission interaction component 6012, and move along the first direction X towards the other end of the logistics transmission interaction component. When the transmission interaction limiting component is in the initial state of avoiding the consumables, the transmission interaction component 6012 carries the consumables to the other end of the logistics transmission interaction component. When the transmission interaction limiting component switches from the initial state to the limiting state, the transmission interaction component 6012 carries the consumables to the interaction position with the transmission interaction limiting component. The transmission interaction limiting component can cooperate with the consumables to limit their movement, allowing the consumables to remain relatively stationary with the transmission interaction limiting component, thereby limiting the consumables to a specific position, facilitating precise interaction between the consumables and other components within the detection equipment.

[0252] The transmission interaction limiting element can move along the second direction Z and switch between an initial state and a limiting state. Alternatively, in other embodiments, the transmission interaction limiting element can oscillate about an axis and switch between an initial state and a limiting state. The key is to limit the consumable material, keeping it relatively stationary.

[0253] In this embodiment, the transmission interaction component 6012 can reciprocate along the first direction X to move the consumable. In other embodiments, the transmission interaction component 6012 can also move only along the first direction X, as long as it can move the consumable. Furthermore, the transmission interaction component 6012 can move in the forward or reverse direction X, depending on the actual usage scenario.

[0254] In the embodiments of this application, the consumables can be well plates, including PCR plates conforming to SBS standards such as 384-well plates and 96-well plates.

[0255] It should be noted that the number of transmission interaction limiting components is not limited. In some embodiments, there may be only one transmission interaction limiting component, which cooperates with the consumable to limit its position. In this case, the consumable may have a groove structure that cooperates with the transmission interaction limiting component. In some embodiments, the transmission interaction limiting component may also be designed as a clamping component that respectively limits and cooperates with both sides of the consumable.

[0256] In addition to the transmission interaction limit component 6020, other types of auxiliary structures, including mechanical structures for clamping, pressing, lifting, and other purposes, can also be installed on the transmission interaction frame 6011.

[0257] Combination Figures 3 to 5 As shown, the transmission interaction limiting device includes a first limiting clamp 6021 and a second limiting clamp 6022. The first limiting clamp 6021 and the second limiting clamp 6022 are spaced apart along a first direction X. Both the first limiting clamp 6021 and the second limiting clamp 6022 can switch between an initial state and a limiting state. When the transmission interaction limiting device switches from the initial state to the limiting state, the first limiting clamp 6021 and the second limiting clamp 6022 can respectively engage with the opposite sides of the consumable for limiting. Using the above design, by having the first limiting clamp 6021 and the second limiting clamp 6022 respectively engage with the sides of the consumable for limiting, the consumable is thus limited to a specific position, offering advantages such as simple structure and reliable limiting structure.

[0258] The first limiting clamping member 6021 and the second limiting clamping member 6022 can switch between the initial state and the limiting state using the same movement method. In some embodiments, both the first limiting clamping member 6021 and the second limiting clamping member 6022 can move by swinging about an axis. In some embodiments, one of the first limiting clamping member 6021 and the second limiting clamping member 6022 can move by swinging about an axis, while the other moves by moving along a second direction Z. The goal is simply to limit the movement of the consumable.

[0259] Combination Figure 3 As shown, the logistics transmission interaction module 6000 also includes a first transmission interaction detection component 6030. The first transmission interaction detection component 6030 is disposed on the transmission interaction frame 6011 and used to detect the position of consumables. The first transmission interaction detection component 6030 is located between a first limiting clamping component 6021 and a second limiting clamping component 6022. The first limiting clamping component 6021 and the second limiting clamping component 6022 can switch from an initial state to a limiting state based on the detection information from the first transmission interaction detection component 6030. By detecting the position of the consumables through the first transmission interaction detection component 6030, and then controlling the first limiting clamping component 6021 and the second limiting clamping component 6022 to switch from the initial state to the limiting state after the consumables have moved to the appropriate position, the consumables can be limited.

[0260] In another embodiment, the consumable is carried on the transmission interaction component 6012 and moves along the first direction X. A first limiting clamp 6021 and a second limiting clamp 6022 are sequentially spaced along the consumable's moving direction. The first limiting clamp 6021 is in an initial state, and the second limiting clamp 6022 is in a limiting state. When the transmission interaction component 6012 carrying the consumable moves to an interaction position with the second limiting clamp 6022, the second limiting clamp 6022 engages with the consumable for limiting. Simultaneously, the first transmission interaction detection component 6030 detects that the consumable has reached a preset working position, and the first limiting clamp 6021 switches from the initial state to the limiting state, working together with the second limiting clamp 6022 to limit the consumable.

[0261] To automate the testing equipment, a controller can be set up. When the first transmission and interaction detection component 6030 detects the consumable, it can transmit the position information of the consumable to the controller. Then, the controller can issue a control signal based on the position information of the consumable, causing the first limit clamping component 6021 and the second limit clamping component 6022 to move.

[0262] Combination Figure 3 and Figure 6As shown, the transmission interaction limiting component 6020 also includes a transmission interaction mounting plate 6023, which is disposed on the transmission interaction frame 6011. The first limiting clamp 6021 and the second limiting clamp 6022 are both swayably disposed on the transmission interaction mounting plate 6023. By adopting the above design and disposing of the first limiting clamp 6021 and the second limiting clamp 6022 on the transmission interaction mounting plate 6023, the integration of the transmission interaction limiting component 6020 is improved, thereby facilitating the integration of the transmission interaction limiting component 6020 with the logistics transmission interaction component.

[0263] In some embodiments, the first transmission interaction detection element 6030 includes a transmitter and a receiver. The transmitter is disposed on the transmission interaction mounting plate 6023, and the receiver is disposed on the side of the transmission interaction frame 6011 away from the transmission interaction mounting plate 6023.

[0264] Combination Figure 6 As shown, the transmission interaction limiting component 6020 also includes a first transmission interaction driver 6024, which is disposed on the transmission interaction mounting plate 6023. The first transmission interaction driver 6024 is drivenly connected to the first limiting clamping member 6021, so that the first limiting clamping member 6021 swings about an axis extending in the second direction Z, where the first direction X and the second direction Z are perpendicular. Using the first transmission interaction driver 6024 to drive the first limiting clamping member 6021 to swing results in a simple structure and easy control.

[0265] The transmission interaction limiting component 6020 also includes a second transmission interaction driver 6025, which is disposed on the transmission interaction mounting plate 6023. The second transmission interaction driver 6025 is drivenly connected to the second limiting clamping member 6022, causing the second limiting clamping member 6022 to swing about an axis extending in the second direction Z, where the first direction X and the second direction Z are perpendicular. Using the second transmission interaction driver 6025 to drive the second limiting clamping member 6022 to swing results in a simple structure and easy control.

[0266] In some embodiments, the first transmission interaction drive 6024 includes an encoder motor, and the second transmission interaction drive 6025 includes an encoder motor. The encoder motor enables semi-closed-loop control, and the microstepping control of the encoder motor enables precise clamping and positioning of the clamping component.

[0267] In other embodiments, other power mechanisms such as push-pull cylinders, rotary cylinders, hydraulic cylinders, electromagnets, or servo motors can be used as the first transmission and interaction drive unit 6024 to drive the first limiting clamping member 6021 and the second limiting clamping member 6022 to swing. However, using a cylinder requires a compressed air source, and using a hydraulic cylinder requires a hydraulic oil source. Using an encoder motor, on the other hand, does not require an additional power source; only electrical energy needs to be converted into the motor's mechanical energy.

[0268] Furthermore, the encoder motor can automatically control the motor's rotation angle, speed, and movement time, thereby automatically controlling the movement time and state of the first limit clamping member 6021 and the second limit clamping member 6022. Simultaneously, the torque feedback during motor shaft rotation can be used to adjust the clamping force during clamping to prevent deformation of consumables. The motor rotation can be considered uniform motion, preventing overshoot.

[0269] In some embodiments, both the first limiting clamp 6021 and the second limiting clamp 6022 are clamping arms, and the style of the clamping arms can be selected independently to adapt to different types and materials of consumables.

[0270] Combination Figure 3 and Figure 6 As shown, the logistics transmission interaction module 6000 also includes a first transmission interaction sensor 6040, which can detect the position information of the first limiting clamp 6021, so that the first limiting clamp 6021 returns to and remains in its initial state. The logistics transmission interaction module 6000 also includes a second transmission interaction sensor 50, which can detect the position information of the second limiting clamp 6022, so that the second limiting clamp 6022 returns to and remains in its initial state. With the above design, when the transmission interaction component 6012 moves the consumables, the first transmission interaction sensor 6040 can detect the position information of the first limiting clamp 6021, and the second transmission interaction sensor 50 can detect the position information of the second limiting clamp 6022, keeping both the first and second limiting clamps 6021 and 6022 in their initial states to avoid interfering with the movement of the consumables.

[0271] Combination Figure 3As shown, the logistics transmission interaction module 6000 also includes a transmission interaction abutment 60, which is disposed on the transmission interaction frame 6011. The transmission interaction abutment 60 and the first limiting clamp 6021 are respectively located on both sides of the transmission interaction frame 6011. The first limiting clamp 6021 and the second limiting clamp 6022 are both provided with abutment slopes. The abutment slopes of the first limiting clamp 6021 and the abutment slopes of the second limiting clamp 6022 can abut against the consumables so that the transmission interaction abutment 60 abuts against the consumables. With the above design, during the clamping process of the first limiting clamp 6021 and the second limiting clamp 6022, the inclined surfaces of the two clamps can generate a pushing force on the consumable, thereby causing the consumable to move towards the transmission interaction abutment 60. Then, the transmission interaction abutment 60, the first limiting clamp 6021 and the second limiting clamp 6022 work together to fix the consumable, making the fixation of the consumable more stable.

[0272] In some embodiments, two logistics transmission interaction components are connected through a logistics docking structure 6016, thereby enabling the logistics transportation of consumables over long distances and expanding the application scenarios of the logistics transmission interaction module 6000.

[0273] It should be noted that the transmission interaction component 6012 of each logistics transmission interaction component can autonomously control the direction of movement, and has strong compatibility when conveying PCR plates / consumables that meet the SBS standard, such as 384-well plates and 96-well plates, to meet the application of multi-station operation. Through program control, it can effectively support both sequential and reverse operation of multi-stations in the testing process.

[0274] When multiple logistics transmission interaction components are used in combination, a certain degree of height deviation between the components in the second direction Z is allowed, which does not affect the transfer of consumables between different logistics transmission interaction components.

[0275] Combination Figure 4 and Figure 5 As shown, the logistics docking structure 6016 includes a plug-in logistics docking post 60161 and a logistics docking hole 60162, which are respectively disposed on two adjacent logistics transmission interaction components. The logistics docking structure 6016 described above is simple in structure and easy to operate.

[0276] Each logistics transmission interaction component is equipped with a logistics docking post 60161 and a logistics docking hole 60162 at both ends, so that any logistics transmission interaction component can be successfully docked during the docking process.

[0277] In other embodiments, other forms of logistics docking structures 6016 may also be used. For example, a magnetically coupled logistics docking structure 6016.

[0278] Combination Figure 4 and Figure 5 As shown, the logistics transmission interaction component also includes a transmission interaction fixing seat 6017. The transmission interaction frame 6011 has transmission interaction fixing seats 6017 at both ends along the first direction X. Logistics docking posts 60161 and logistics docking holes 60162 are respectively located at the transmission interaction fixing seats 6017 at both ends of the transmission interaction frame 6011. By adjusting the relative positions of the transmission interaction fixing seats 6017 and the transmission interaction frame 6011, the relative positions of the logistics docking structure 6016 and the transmission interaction frame 6011 can be adjusted, thereby improving the success rate of alignment between the two logistics transmission interaction components and increasing alignment efficiency.

[0279] Combination Figure 4 As shown, the logistics transmission interaction component also includes a transmission interaction drive wheel 60111 and a transmission interaction driven wheel 60112, which are spaced apart along a first direction X on the transmission interaction frame 6011. The transmission interaction component 6012 includes a transmission interaction conveyor belt, which is rotatably fitted onto the transmission interaction drive wheel 60111 and the transmission interaction driven wheel 60112. With this design, the transmission interaction drive wheel 60111 and the transmission interaction driven wheel 60112 drive the transmission interaction conveyor belt to rotate, resulting in a simple structure and high reliability.

[0280] In some embodiments, the logistics transmission interaction component further includes a motor and a transmission interaction drive shaft 6019 connected to the output shaft of the motor. The transmission interaction drive wheel 60111 is sleeved on the transmission interaction drive shaft 6019, thereby achieving rotation. Furthermore, a transmission interaction tension wheel 60113 is provided on the rotation path of the transmission interaction conveyor belt. The transmission interaction tension wheel 60113 provides tension to the transmission interaction conveyor belt, preventing it from detaching from the transmission interaction drive wheel 60111 and the transmission interaction driven wheel 60112.

[0281] Among them, the conveyor belt for transmission and interaction can be a flat belt, a V-belt, or other types of belt.

[0282] Combination Figure 4As shown, the logistics transmission interaction component also includes a transmission interaction support 6015, which is disposed on the transmission interaction frame 6011. The transmission interaction support 6015 can cooperate with the transmission interaction conveyor belt to provide support for the consumables. By using the transmission interaction support 6015 to provide support for the transmission interaction conveyor belt, the support force can be provided to the consumables. This can be applied to scenarios where the consumables are heavy, making the consumables more stable when moving on the transmission interaction conveyor belt.

[0283] In some embodiments, the transmission interaction frame 6011 is provided with two transmission interaction conveyor belts spaced apart along the Y direction, and each transmission interaction conveyor belt is supported by a transmission interaction support member 6015.

[0284] Furthermore, both ends of the transmission drive shaft 6019 are fitted with transmission drive wheels 60111, each corresponding to a transmission conveyor belt. At the other end of the transmission frame 6011, two transmission driven wheels 60112 are respectively mounted on the transmission frame 6011, and there is no transmission structure between the two transmission driven wheels 60112. The space between the two transmission driven wheels 60112 can interact with pallet-type mechanical transfer devices.

[0285] Combination Figure 4 As shown, the logistics transmission interaction component also includes a transmission interaction guide 6018, which is disposed on the transmission interaction frame 6011. The transmission interaction component 6012 has transmission interaction guides 6018 on both sides along the third direction Y, which is perpendicular to the first direction X. Guide ramps are provided at both ends of the transmission interaction guide 6018 along the first direction X. With this design, the guide ramps can guide the consumables, reducing the offset of the consumables during transport on different logistics transmission interaction components, ensuring that the consumables are held in an appropriate position on the transmission interaction conveyor belt and move along the direction of movement of the transmission interaction conveyor belt.

[0286] See Figure 8 In some embodiments, the logistics transmission interaction module 6000 includes a drying transmission mechanism 6300, which moves consumables through a drying preparation station. The drying transmission mechanism 6300 is connected between the liquid spraying position of the reaction plate 0002 and other preset working positions, and is used to transport the sprayed reaction plate 0002 to the drying preparation station or other preset working positions. Specifically, the drying transmission mechanism 6300 is connected between the sample transfer station and the reagent addition station 20011, and is used to transport the sprayed reaction plate 0002 to the drying preparation station and / or the reagent addition station 20011.

[0287] Specifically, the drying and conveying mechanism 6300 is connected between the spray position of the reaction plate 0002 and other preset working positions. It transports the sprayed reaction plate 0002 to the drying preparation position or other preset working positions and can also move consumables through the drying preparation position. The system dynamically matches the start and stop of the sample pipetting arm 3000, its moving speed, the aspiration and spraying action of the sample pipetting head module 1000, and the start-up preparation of the drying module 9000 by real-time feedback transmission interaction component 6012 and the position signal of the drying and conveying mechanism 6300. This effectively avoids the problem of timing misalignment or connection gaps between consumable transfer, pipetting operation, and drying operation, ensuring the synchronization of each link and guaranteeing the efficient and continuous operation of the entire pipetting and plate making process.

[0288] Through the above settings, the precise transfer and collaborative control of the logistics transmission interaction module 6000 enables rapid connection from sample pipetting to the drying preparation station, ensuring the stability of plate-making quality and improving plate-making efficiency.

[0289] See Figure 8 In some embodiments, the drying module 40000 includes a drying chamber 40100 and a drying pick-and-place mechanism 40200. The drying chamber 40100 is used to store and dry consumables; the drying pick-and-place mechanism 40200 includes a drying drive mechanism, which includes a pick-and-place sliding seat 40243 that is slidably arranged in the horizontal direction. The pick-and-place sliding seat 40243 is provided with a pick-and-place lifting part 40244 on the side facing the drying chamber 40100. The pick-and-place lifting part 40244 is used to carry consumables. The drying drive mechanism is used to drive the consumables to be picked up and placed inside the drying chamber 40100; wherein, the drying transmission mechanism 6300 is used to transport the consumables to the side of the pick-and-place sliding seat 40243 for interaction with the pick-and-place sliding seat 40243.

[0290] The above configuration addresses the technical shortcomings of existing sample drying equipment that relies on manual handling of trays 81332. The drying chamber 40100, as the core cavity for sample drying, provides storage space for consumables and a drying function module, enabling uniform and controllable drying of the placed consumables. The drying handling mechanism 40200, as the core actuator for automated handling, has a handling sliding seat 40243 that slides along a preset composite trajectory. The handling lifting part 40244, located on the end of the handling sliding seat 40243 facing the drying chamber 40100, precisely aligns with the tray inlet and outlet of the drying chamber 40100. The displacement of the handling sliding seat 40243 drives the handling lifting part 40244 to deliver consumables into a designated position inside the drying chamber 40100, or to remove dried consumables from inside the drying chamber 40100, effectively replacing manual operation and improving the automation level and operational safety of the drying process.

[0291] See Figure 9 and Figure 10The drying box 40100 also includes multiple winding components 40121. A notch 40110 is provided on the outer wall of the drying box 40100 facing the pick-up and place support 40244. The drying box 40100 includes a drying adjustment mechanism 40120, which includes multiple winding components 40121. A blocking component 40122 is provided between the multiple winding components 40121. The blocking component 40122 is used to block the notch 40110. A pick-up and place opening 40123 is provided on the blocking component 40122. When the multiple winding components 40121 rotate, the pick-up and place opening 40123 rises and falls relative to the drying box 40100 to drive the blocking component 40122 to block the notch 40110, or the pick-up and place opening 40123 is opposite to the notch 40110.

[0292] In some embodiments, the drying oven 40100 is provided with a plurality of winding members 40121, and a drying adjustment mechanism 40120 is connected between adjacent winding members 40121. The drying adjustment mechanism 40120 adopts a curtain-type flexible structure and covers the notch 40110 opened on the outer wall of the drying oven 40100 facing the pick-up and place support part 40244, so as to block and close the notch 40110 and reduce the heat leakage inside the drying oven 40100.

[0293] In some embodiments, the shielding member 40122 is provided with a pick-up and put-out port 40123 for the pick-up and put-out lifting part 40244 to enter and exit. When multiple winding members 40121 are driven to rotate, the shielding member 40122 is displaced with the winding or releasing action of the winding members 40121, which drives the pick-up and put-out port 40123 to complete the lifting and lowering action relative to the drying installation member 40140 in the drying box 40100, so that the pick-up and put-out port 40123 can be accurately aligned with the drying installation member 40140 at different heights, and cooperate with the pick-up and put-out lifting part 40244 to complete the pick-up and put-out operation of consumables.

[0294] In some embodiments, compared to the entire door of a traditional drying oven 40100, the curtain-type shield 40122 only has a partial opening through the loading / unloading port 40123, significantly reducing heat loss from the drying oven 40100. The heat loss rate can be reduced by more than 60%, while preventing a large amount of external cold air from entering, ensuring temperature uniformity inside the oven. The loading / unloading port 40123 rises and falls with the winding component 40121, matching the height of the multi-layer drying mounting component 40140, eliminating the need for manual position adjustment, and adapting to the automatic loading / unloading action of the drying loading / unloading mechanism 40200.

[0295] In some embodiments, the flexible curtain-type shield 40122 replaces the rigid door, reducing the structural weight of the drying oven 40100, and the energy consumption of the winding drive is much lower than that of the opening and closing drive of the rigid door.

[0296] In some embodiments, a high-temperature resistant silicone magnetic sealing strip is added to the periphery of the pick-up / placement opening 40123. When the pick-up / placement opening 40123 is aligned with the pick-up / placement support part 40244, the strip adheres to the outer wall of the pick-up / placement support part 40244, forming a seal. After pick-up / placement is completed, the strip automatically closes, sealing the gap in the pick-up / placement opening 40123.

[0297] In some embodiments, brush-type sample cleaning sealing strips 20141 are added to both sides of the notch 40110 on the drying oven 40100. The side of the shielding member 40122 is embedded in the brush, which not only does not affect its lifting and lowering, but also seals the gap between the shielding member 40122 and the oven body, reducing heat loss. The single-layer door curtain is upgraded to a double-layer insulated door curtain, with a 5mm-8mm air layer reserved between the two layers of door curtain, further improving the heat insulation effect. At the same time, the outer layer can prevent scratches and extend the service life.

[0298] In some embodiments, a photoelectric encoder and an electromagnetic brake are installed on the shaft of the take-up component 40121. The encoder provides real-time feedback on the shaft rotation angle, precisely controlling the lifting stroke of the shielding component 40122 with a positioning accuracy of ≤±0.5mm. The electromagnetic brake locks the take-up component 40121 after the loading / unloading port 40123 aligns with the drying mounting component 40140, preventing displacement. Multiple take-up components 40121 are driven by the same servo motor, replacing independent motors, ensuring the synchronous rotation of the upper and lower take-up components 40121 and preventing the drying adjustment mechanism 40120 from tilting. Alternatively, guide wheels can be added next to the take-up component 40121 to limit the lifting trajectory of the shielding component 40122.

[0299] In some embodiments, a miniature vision sensor is installed above the notch 40110 of the drying chamber 40100 to identify the position coordinates of the drying mounting component 40140 and feed them back to the drive control system of the winding component 40121 to automatically calibrate the lifting height of the take-up and put-out port 40123 and adapt to the position deviation of the drying mounting component 40140.

[0300] In some embodiments, an infrared sensor is added inside the drying chamber 40100 to detect whether the pick-up and drop-off support 40244 has been completely retracted, and the pick-up and drop-off port 40123 is driven to rise and fall only after confirmation. A dustproof and heat-insulating cover is installed on the outside of the take-up component 40121 to prevent the high-temperature airflow inside the drying chamber 40100 from directly contacting the bearings or motor of the take-up component 40121, thereby extending the life of the drive components. Heat dissipation holes are provided on the cover, and a small cooling fan is used to ensure the normal operating temperature of the motor of the take-up component 40121.

[0301] In some embodiments, multiple loading / unloading ports 40123 are provided on the shielding member 40122. The winding member 40121 can drive the shielding member 40122 to move up and down in a step-by-step manner, simultaneously loading and unloading multiple layers of consumables, thus improving processing efficiency. A miniature air knife is installed on the top of the notch 40110 on the drying chamber 40100. When the loading / unloading port 40123 is raised to the top, the air knife sprays high-pressure hot air to blow away dust from the surface of the shielding member 40122, preventing cross-contamination. The hot air for the air knife can be taken from the circulating hot air inside the drying chamber 40100, eliminating the need for additional heating.

[0302] In some embodiments, the drive system of the take-up component 40121 is linked to the control system of the drying pick-and-place mechanism 40200. When the pick-and-place lifting part 40244 moves to the front of the notch 40110, a signal is automatically sent, and the take-up component 40121 drives the pick-and-place opening 40123 to rise and fall to the corresponding height. After pick-and-place is completed, the pick-and-place lifting part 40244 is removed. A tension sensor is installed on the shield 40122 to monitor the tension of the shield 40122 in real time. When the tension is abnormal, an alarm is automatically triggered and the equipment stops operating. At the same time, the number of times the pick-and-place opening 40123 rises and falls and the running time of the take-up component 40121 are recorded for easy equipment maintenance.

[0303] See Figures 8-10 The drying chamber 40100 is equipped with a drying mounting component 40140. The drying mounting component 40140 is provided with multiple sliding grooves 40141 spaced apart in the vertical direction, and consumables are placed in the sliding grooves 40141. The drying drive mechanism includes a vertical drying drive unit and a horizontal drying drive unit 40240. The vertical drying drive unit includes a drying support component 40210 and a drying counterweight seat 40230. The drying support component 40210 includes a drying slide rod 40220 and a drying timing belt 40250. The drying counterweight seat 40230 is slidably mounted on the drying slide rod 40220. The horizontal drying drive unit 40240 is connected to the drying timing belt 40250.

[0304] In some embodiments, the drying mounting component 40140 is located inside the drying chamber 40100 and serves as the main support for consumables. The slide 40141 is a mounting carrier that supports the consumables, and its position determines the lifting height of the pick-up and drop-off port 40123. The consumables are adapted to the size of the slide 40141 to support the biological sample plate 0001 to be dried, which slides into or out of the drying mounting component 40140 along the slide 40141 to complete the pick-up and drop-off.

[0305] In some embodiments, the chute 40141 limits the displacement of consumables through a slot-type structure. Compared to a simple flat partition, this prevents the tray from sliding or tipping due to airflow disturbances or slight vibrations within the drying chamber 40100, protecting the sample plates 0001, especially the samples in 96-well or 384-well precision plates, from spillage. The linear trajectory of the chute 40141 matches the pushing and pulling action of the pick-and-place lifting part 40244, facilitating the automatic pushing or pulling of the tray by the drying pick-and-place mechanism 40200, replacing manual placement and improving automation adaptability. Multiple chute 40141s spaced apart can be used to place consumables in layers, fully utilizing the internal longitudinal space of the drying chamber 40100 and increasing the single-batch drying capacity.

[0306] In some embodiments, the traditional fixed-interval chutes 40141 are upgraded to a modular adjustable structure. A long, narrow slide rail is provided on the drying mounting component 40140, and the chutes 40141 are connected to the slide rail via sliders. The spacing between adjacent chutes 40141 can be adjusted by tightening the positioning bolts. Simultaneously, replaceable adapter strips, such as silicone, are added to the inner wall of the chutes 40141. By replacing strips of different widths, it is compatible with sample board 0001 trays of different sizes, such as 6-hole, 24-hole, and 96-hole trays.

[0307] In some embodiments, an elastic buckle or magnetic positioning block is added to the end of the slide 40141. When the consumable is pushed to the preset position along the slide 40141, the buckle automatically engages with the slot on the edge of the tray, or the magnetic block attracts the tray and locks the tray position. When the pick-up and drop-off lifting part 40244 picks up or drops the tray, the buckle is opened by the ejector pin, or the magnetic attraction is released by power failure, thereby unlocking the tray and preventing it from shifting during the drying process.

[0308] In some embodiments, a high-temperature resistant and wear-resistant PTFE (polytetrafluoroethylene) friction-reducing layer is adhered to the inner wall of the chute 40141 to reduce friction during tray pushing and pulling. Simultaneously, an elastic buffer pad is added at the inlet of the chute 40141; when the tray is pushed in, it first contacts the buffer pad to cushion the impact and prevent sample vibration. Miniature photoelectric sensors are installed at the inlet and end of the chute 40141, respectively. The inlet sensor detects whether the tray pushed by the pick-and-place lifting part 40244 is in place, and the end sensor confirms whether the tray is fully pushed in. Sensor signals are fed back to the control system of the drying pick-and-place mechanism 40200. If the tray is not in place, the pick-and-place lifting part 40244 is automatically triggered for fine-tuning, avoiding manual intervention.

[0309] In some embodiments, a miniature electric push rod is added to the drying mounting component 40140 at the position corresponding to each slide 40141. When the pick-and-place lifting part 40244 pushes the tray into the slide 40141 to half position, the electric push rod pushes forward simultaneously to assist the tray in smoothly entering the slide. During pick-and-place, the electric push rod first pulls the tray backward to the entrance of the slide 40141, and then the pick-and-place lifting part 40244 pulls it out, reducing the load on the pick-and-place lifting part 40244 and improving the stability of pick-and-place.

[0310] See Figure 8 and Figures 11-12 The horizontal drying drive unit 40240 includes a drying base 40241, which is fixedly mounted on the drying synchronous belt 40250. A drying track 40242 is provided on the drying base 40241, and a pick-up and put-down sliding seat 40243 is slidably mounted on the drying track 40242.

[0311] In some embodiments, the drying slide bar 40220 is fixedly disposed on the drying support member 40210 and slidably connected to the drying counterweight seat 40230. When the drying timing belt 40250 moves, the drying timing belt 40250 drives the horizontal drying drive unit 40240 to move linearly in the direction of gravity.

[0312] In some embodiments, the drying support 40210 serves as the basic load-bearing structure for the drying pick-and-place mechanism 40200, and fixes the drying slide bar 40220, providing vertical support and a stable foundation for the rotation of the drying slide bar 40220 and the lifting and lowering of the drying counterweight 40230. The drying slide bar 40220 is rotatably mounted on the drying support 40210 and threadedly connected to the drying counterweight 40230, converting rotational motion into linear motion. That is, the rotation of the drying slide bar 40220 drives the drying counterweight 40230 to rise and fall along its axial direction, thereby synchronously driving the horizontal drying drive unit 40240 to rise and fall.

[0313] In some embodiments, the drying counterweight 40230 is threadedly engaged with the drying slide bar 40220, supports the horizontal drying drive unit 40240, and transmits lifting power, serving as the power transmission carrier between the drying slide bar 40220 and the horizontal drying drive unit 40240. The horizontal drying drive unit 40240 is fixed to the drying counterweight 40230, with the pick-and-place slide 40243 as its core component. The horizontal drying drive unit 40240 supports the pick-and-place slide 40243, provides horizontal sliding guidance, and rises and falls with the drying counterweight 40230, while also providing support for the horizontal movement of the pick-and-place slide 40243. The pick-and-place slide 40243 is slidably disposed on the horizontal drying drive unit 40240, and the pick-and-place lifting part 40244 drives the consumables horizontally in and out of the drying chamber 40100. During the pick-up and place operation, the pick-up and place lifting part 40244 first rises and falls with the drying counterweight 40230 to the height of the corresponding slide 40141, and then slides horizontally to complete the pick-up and place operation.

[0314] In some embodiments, the drying slide bar 40220 drives the drying counterweight 40230 to achieve vertical lifting and lowering, matching the slide grooves 40141 of different heights within the drying chamber 40100, and the loading and unloading slide seat 40243 to achieve horizontal loading and unloading, forming a two-dimensional motion of lifting and lowering and horizontal movement. This completely replaces the manual actions of adjusting the height and pushing the tray, fundamentally solving the problem of automated loading and unloading. Compared with cylinder lifting, the drying slide bar 40220 transmission eliminates displacement deviation caused by air pressure fluctuations, resulting in higher positioning accuracy and stepless speed regulation, adapting to the smooth lifting and lowering of consumables of different weights. The vertical transmission structure of the drying slide bar 40220 and the drying counterweight 40230 occupies little space and can be arranged close to the drying chamber 40100, saving laboratory equipment floor space.

[0315] In some embodiments, two optical axis guide rods are added parallel to the drying slide rod 40220 on the drying support 40210, and the drying counterweight 40230 is sleeved on the guide rods to restrict the circumferential rotation of the drying counterweight 40230, so that the lifting and lowering deviation is ≤±0.5mm. The surface of the guide rods is coated with a wear-resistant coating to reduce sliding friction resistance.

[0316] In some embodiments, for heavy load scenarios, the single drying slide bar 40220 is upgraded to a symmetrical arrangement of two drying slide bars 40220. The two drying slide bars 40220 are driven to rotate synchronously by the same servo motor through the drying synchronous belt 40250, which distributes the load, avoids jamming caused by overload of a single bar, and improves lifting stability.

[0317] In some embodiments, a miniature laser positioning sensor and a vision camera are installed on the horizontal drying drive unit 40240. The laser sensor calibrates the horizontal alignment accuracy between the pick-up and place slide 40243 and the slide rail 40141, and the vision camera calibrates the contact accuracy between the tray and the pick-up and place slide 40243. The data is fed back to the control system, which automatically fine-tunes the position of the pick-up and place slide 40243 to ensure an alignment accuracy of ≤±0.2mm. Photoelectric limit switches are added to both ends of the sliding guide rail of the horizontal drying drive unit 40240 to limit the maximum stroke of the pick-up and place slide 40243, preventing the tray from hitting the inner wall of the drying chamber 40100 due to over-pushing or over-pulling, which would cause the tray to detach from the pick-up and place slide 40243.

[0318] In some embodiments, a retractable dustproof cover is installed on the outside of the drying slide bar 40220. One end of the cover is fixed to the drying support 40210 and the other end is fixed to the drying counterweight 40230. The cover extends and retracts synchronously with the drying counterweight 40230, isolating dust and sample volatiles and preventing thread jamming. A grease filling port is added to the inside of the cover, allowing for periodic addition of high-temperature grease to extend the service life of the drying slide bar 40220. The horizontal drying drive unit 40240 is designed as a quick-release modular structure, connected to the drying counterweight 40230 via a flange. Disassembly does not require disassembling the drying slide bar 40220 or the drying counterweight 40230, facilitating the maintenance and replacement of the horizontal drying drive unit 40240.

[0319] See Figures 12-13 The horizontal drying drive unit 40240 includes a first rotating shaft 40245 and a second rotating shaft 40246. A drying conveyor belt 40247 is connected between the first rotating shaft 40245 and the second rotating shaft 40246. A pick-and-place sliding seat 40243 is fixedly connected to the drying conveyor belt 40247. When the first rotating shaft 40245 and the second rotating shaft 40246 rotate, the drying conveyor belt 40247 drives the pick-and-place sliding seat 40243 and consumables to slide back and forth along the drying track 40242.

[0320] In some embodiments, the drying base 40241 is fixed to the drying counterweight 40230, providing an installation reference for the drying track 40242. The drying track 40242 is disposed on the drying base 40241, providing a sliding guide for the pick-up and place sliding seat 40243 and defining the movement trajectory of the pick-up and place sliding seat 40243, ensuring that the pick-up and place lifting part 40244 is accurately aligned with the slide groove 40141 of the drying chamber 40100. The pick-up and place sliding seat 40243 slides in cooperation with the drying track 40242, and the pick-up and place lifting part 40244 drives the consumables horizontally in and out of the drying chamber 40100, completing the push-in or pull-out action of the consumables.

[0321] In some embodiments, the single drying track 40242 is upgraded to two parallel and symmetrically arranged drying tracks 40242. The pick-and-place sliding seat 40243 simultaneously slides with both drying tracks 40242, forming a double-guide structure. This restricts the lateral tilting or displacement of the pick-and-place sliding seat 40243, ensuring a horizontal sliding deviation of ≤±0.2mm. The drying tracks 40242 utilize precision linear guides, such as ball bearing slides, instead of ordinary guides, reducing sliding resistance by more than 70% and improving smoothness. The drying tracks 40242 are made of stainless steel with a nitrided surface treatment, enhancing wear resistance and corrosion resistance. High-temperature resistant PEEK sliders are embedded in the mating surfaces of the pick-and-place sliding seat 40243 and the drying tracks 40242, replacing direct metal contact, preventing jamming at high temperatures, and reducing noise.

[0322] In some embodiments, the drying track 40242 is designed as a modular splicing structure, such as splicing 2-3 short drying track segments 40242. The total length is adjusted by increasing or decreasing the number of drying track segments 40242 to accommodate the sliding groove 40141 of the drying box 40100 with different depths of 50-300mm. At the same time, a stroke adjustment stop is installed on the drying base 40241, which allows for manual or electric fine-tuning of the maximum stroke of the lifting and placing sliding seat 40243 to prevent overtravel.

[0323] In some embodiments, the drive system of the pick-and-place sliding seat 40243 is linked with the lifting and lowering system of the pick-and-place port 40123 of the drying oven 40100 and the alignment system of the sliding groove 40141 of the drying installation component 40140. Before the pick-and-place sliding seat 40243 slides, it is confirmed that the pick-and-place port 40123 is aligned and the sliding groove 40141 is free of foreign objects. After the sliding is completed, an automatic feedback signal is sent to indicate that the seat is in place.

[0324] See Figure 12 The logistics transmission interaction module 6000 also includes a drying transmission mechanism 6300, which is used to transport consumables to one side of the horizontal drying drive unit 40240.

[0325] In some embodiments, the first rotating shaft 40245 and the second rotating shaft 40246 are rotatably mounted on the horizontal drying drive unit 40240, arranged in pairs, and provide support and driving power for the drying conveyor belt 40247. The drying conveyor belt 40247 circulates, thereby further pulling the pick-and-place sliding seat 40243 to slide. The drying conveyor belt 40247 is wound between the two rotating shafts and fixedly connected to the pick-and-place sliding seat 40243, converting the rotational motion of the rotating shafts into the linear motion of the pick-and-place sliding seat 40243. It is the core carrier of power transmission and defines the sliding trajectory of the pick-and-place sliding seat 40243.

[0326] In some embodiments, the pick-and-place sliding seat 40243 is fixed to the drying conveyor belt 40247 and slidably engages with the drying track 40242. It drives the consumables to slide reciprocally along the drying track 40242, moving synchronously with the drying conveyor belt 40247 to complete the pushing and pulling actions of the tray. Compared to rigid transmissions such as gears and lead screws, the drying conveyor belt 40247 transmission has no mechanical meshing gaps, low operating noise, and can buffer the impact during start-up and stop, reducing the vibration of samples within the consumables. The drying conveyor belt 40247 can adapt to longer travel distances on the drying track 40242. Compared to short-stroke cylinder drives, it can meet the pick-and-place requirements of the chute 40141 in drying boxes of different depths, and the transmission efficiency is not affected by the travel length.

[0327] In some embodiments, the transmission combination of the drying conveyor belt 40247 and the rotating shaft is lightweight and does not significantly increase the load on the drying counterweight 40230, resulting in smoother lifting and lowering drive in conjunction with the drying slide bar 40220. The drying conveyor belt 40247 is fixedly connected to the pick-up and place slide 40243, ensuring that the pick-up and place slide 40243 moves at a constant speed throughout the entire process, avoiding jamming and improving the stability of pallet picking and placing.

[0328] In some embodiments, an adjustable slide rail and a tension bolt are added to the second rotating shaft 40246 on the horizontal drying drive unit 40240. The second rotating shaft 40246 can move along the slide rail. By tightening the tension bolt, the distance between the two rotating shafts can be adjusted to eliminate the elastic slack of the drying conveyor belt 40247 in real time, ensuring that the transmission is slip-free. At the same time, a tension sensor is added to monitor the tension of the drying conveyor belt 40247 in real time. When the tension is lower than the threshold, an alarm is automatically triggered, prompting the adjustment of the tension.

[0329] In some embodiments, the ordinary drying conveyor belt 40247 is upgraded to a polyurethane drying synchronous belt 40250, and the shaft is replaced with a toothed synchronous pulley. The drying synchronous belt 40250 meshes with the synchronous pulley for transmission, eliminating the elastic slippage of the drying conveyor belt 40247 and improving the positioning accuracy to ±0.1mm, which is fully compatible with the precise picking and placing of 384-hole trays. An electromagnetic brake and a photoelectric encoder are added to the drive motor end of the first shaft 40245. The encoder provides real-time feedback on the shaft rotation angle, thereby precisely controlling the stroke of the drying conveyor belt 40247. The brake assembly immediately locks the shaft after stopping or after picking and placing, preventing accidental displacement of the picking and placing sliding seat 40243.

[0330] In some embodiments, the pick-and-place lifting part 40244 can adopt two structural forms to realize the transfer of consumables, adapting to the transfer needs of consumables of different specifications and weights. The shovel-type structure is suitable for consumables with flat bottoms and heavy weights. The pick-and-place lifting part 40244 is a high-strength stainless steel shovel body. The upper surface of the shovel body is equipped with an anti-slip silicone pad. After being horizontally inserted under the consumables, it can disperse the pressure on the tray by increasing the contact area and avoid the tray deformation. In addition, the edges of the shovel body are rounded to prevent scratching the bottom of the tray.

[0331] In some embodiments, the motorized gripper structure is suitable for lightweight consumables with side gripping positions. The motorized gripper is servo-driven, and the gripping force is adjustable, which can both clamp the tray to prevent slippage and avoid excessive gripping force that could deform the tray. Wear-resistant and anti-slip pads are attached to the inside of the gripper to further improve gripping stability, and the opening and closing stroke of the gripper is adjustable to accommodate consumables of different widths.

[0332] In some embodiments, to simplify the equipment structure and reduce intermediate steps, this solution provides a simplified implementation. The drying transmission mechanism 6300, which consists of multiple sub-conveying units 6310, is removed, leaving only the drying chamber 40100 and the drying pick-and-place mechanism 40200. The pick-and-place sliding seat 40243 of the drying pick-and-place mechanism 40200 is upgraded to a mechanism with bidirectional linear running capability, whose running trajectory covers the entire stroke from the fixed station to the pick-and-place port 40123 of the drying chamber 40100.

[0333] In some embodiments, during the feeding stage, the user places the consumables to be dried at a fixed station preset in the laboratory, such as a designated area on the operating table. The pick-and-place sliding seat 40243 drives the pick-and-place lifting part 40244 to move to the fixed station. After receiving the consumables, they are directly fed into the drying installation part 40140 inside the drying chamber 40100 along the trajectory.

[0334] During the feeding stage, after the sample is dried, the pick-and-place sliding seat 40243 drives the pick-and-place lifting part 40244 to take out the consumables from the drying box 40100 and move in the opposite direction along the original trajectory to the fixed station, where the user can take away the dried consumables.

[0335] Compared to the version including the drying conveyor mechanism 6300, this form reduces the intermediate steps of consumables, sub-conveyor 6310, drying counterweight 40230, and pick-and-place lifting unit 40244, shortening the time for single-batch pallet transfer by more than 40%. At the same time, the overall footprint of the equipment is reduced by 30%, making it more suitable for the space layout of small laboratories. The fixed workstation design also facilitates centralized operation by users, reducing the complexity of manual intervention.

[0336] See Figure 8 and Figure 9The drying and conveying mechanism 6300 is configured as two spaced sub-conveyor sections 6310, with consumables located on the two sub-conveyor sections 6310; or the drying and conveying mechanism 6300 is configured as an AGV (Automated Guided Vehicle).

[0337] In some embodiments, the drying conveying mechanism 6300 is a consumable conveying unit independent of the drying chamber 40100 and the drying pick-and-place mechanism 40200. It connects the laboratory pre-process with the horizontal drying drive unit 40240, providing consumables to be dried to the horizontal drying drive unit 40240, and is the upstream connection point of the automated process. Sub-conveying units 6310 are arranged at intervals to precisely convey the tray to the grasping position of the pick-and-place lifting unit 40244 of the horizontal drying drive unit 40240.

[0338] In some embodiments, the sub-conveying unit 6310 can simultaneously carry multiple consumables, enabling continuous feeding without the need for manual handling of each tray to the horizontal drying drive unit 40240, thus improving the efficiency of high-throughput sample processing. The sub-conveying unit 6310 can also serve as a temporary buffer area for consumables. When the horizontal drying drive unit 40240 or the drying chamber 40100 is not yet ready, the tray can be temporarily stored in the sub-conveying unit 6310 to avoid interruptions in the preceding process.

[0339] In some embodiments, the sub-conveyor 6310 uses a roller or belt drive. The roller surface is covered with an anti-slip silicone sleeve, and the belt is a drying synchronous belt 40250 with positioning grooves to prevent slippage and deviation during pallet transport. For heavy pallets, the sub-conveyor 6310 is equipped with auxiliary lifting wheels to distribute the pallet weight and reduce transport resistance.

[0340] In some embodiments, the sub-conveying section 6310 is designed as a width-adjustable structure, with the spacing between the two conveying sides adjusted by lead screws to accommodate consumables with widths ranging from 100mm to 300mm. The adjustment is locked via scale lines, eliminating the need for repeated calibration. Replaceable limiting strips are added to the inner side of the conveying sides to accommodate pallets of different thicknesses, from 5mm to 20mm, preventing pallet tipping.

[0341] In some embodiments, a photoelectric sensor and a weight sensor are installed on each sub-conveyor 6310. The photoelectric sensor is used to detect whether there is a pallet or whether the pallets are stacked. The weight sensor is used to detect whether the pallets are empty or overloaded. If stacking, empty, or overloaded is detected, the conveying is stopped immediately and an alarm is triggered. The end sub-conveyor 6310 is equipped with a vision sensor to identify the placement angle of the pallets. If the tilt angle is >2°, the correction cylinder is automatically triggered to adjust the position of the pallets to ensure that the horizontal drying drive unit 40240 can grasp them smoothly.

[0342] In some embodiments, the drying conveying mechanism 6300 establishes a linkage control with the horizontal drying drive unit 40240, the drying counterweight 40230, and the drying chamber 40100. After the sub-conveyor 6310 conveys the tray to the end and completes the alignment, the sensor sends a tray ready signal. The drying counterweight 40230 drives the horizontal drying drive unit 40240 to descend to the height of the sub-conveyor 6310, the pick-and-place sliding seat 40243 extends along the drying track 40242, and the pick-and-place lifting part 40244 receives the tray.

[0343] See Figure 11 In some embodiments, the drying module 40000 further includes a drying lifting mechanism 40400, which includes a drying lifting drive 40410. The drying lifting drive 40410 is located on the side of the drying transmission mechanism 6300 near the horizontal drying drive unit 40240. The output end of the drying lifting drive 40410 is provided with a drying support 40420. The drying support 40420 is used to support consumables, and the drying lifting drive 40410 is used to adjust the vertical height of the consumables to drive the consumables to detach from the drying transmission mechanism 6300.

[0344] In some embodiments, the drying lifting drive 40410 is located at the output end of the sub-conveying section 6310, has a linear lifting stroke and provides lifting power for adjusting the height of consumables. The drying support 40420 is connected to the output end of the drying lifting drive 40410, carries consumables, and provides space for the placement and removal of the support 40244 under the tray.

[0345] In some embodiments, the drying lifting drive 40410 compensates for the height difference between the sub-conveying section 6310 and the horizontal drying drive unit 40240, eliminating the need for the pick-and-place lifting section 40244 to extend below the conveying surface of the sub-conveying section 6310, thus avoiding interference with the transmission components of the sub-conveying section 6310 and simplifying the pick-and-place path. Furthermore, the drying lifting drive 40410 has a faster lifting response than the lead screw lifting mechanism, enabling rapid tray height adjustment and adapting to continuous processing of high-throughput samples.

[0346] In some embodiments, a positioning groove is added to the support surface of the drying support 40420 to match the protrusion 14211 or edge of the bottom of the tray, or an adjustable limiting edge is added to limit the left and right displacement of the tray during lifting. An anti-slip silicone pad with a diamond pattern is pasted on the support surface to increase the friction between the tray and the drying support 40420 and prevent slippage during lifting.

[0347] In some embodiments, the drying lifting drive 40410 drives the drying support 40420 to rise to a preset height and sends a lifting-in signal. Upon receiving the signal, the horizontal drying drive unit 40240 drives the pick-and-place support 40244 to horizontally insert under the tray. The pressure sensor on the pick-and-place support 40244 detects the weight of the tray, confirms receipt, and sends a tray receipt signal. Finally, the drying lifting drive 40410 drives the drying support 40420 to descend and reset, awaiting the delivery of the next tray.

[0348] In some embodiments, the drying support 40420 is designed as a modular adjustable structure, and the spacing of the limiting edge of the support surface is adjusted by a screw to accommodate consumables of different specifications. The support height of the drying support 40420 can be adjusted by adding shims of different thicknesses to accommodate trays with different bottom thicknesses, thereby ensuring that the lifting part 40244 can be inserted smoothly.

[0349] See Figure 10 The drying oven 40100 also includes a heating element 40160 and a blowing element 40170. The heating element 40160 is located inside the drying oven 40100; the heating element 40160 is located on the air outlet side of the blowing element 40170.

[0350] In some embodiments, the blowing element 40170 drives the airflow through the heating element 40160 to form directional hot air. Compared with natural convection drying, the heat transfer efficiency is improved by more than 40%, significantly shortening the drying time. The heating element 40160 is located at the output end of the blowing element 40170. The airflow is heated by the heating element 40160 before entering the cavity, avoiding a sudden drop in local temperature caused by cold air blowing directly on the sample, and ensuring temperature stability in the initial stage of drying.

[0351] In some embodiments, a guide vane is added inside the drying chamber 40100. The guide vane has a multi-directional flow design, which divides the hot air output from the blowing element 40170 into multiple airflows, which are directed to the drying mounting elements 40140 at different heights. At the same time, a return air vent is opened at the bottom of the drying chamber 40100. After the hot air flows through the sample area, it flows back to the input end of the blowing element 40170 from the return air vent, forming a closed-loop thermal cycle of heating, air supply, sample area, return air, and reheating. The temperature difference inside the chamber can be controlled within ±1℃.

[0352] In some embodiments, the heating element 40160 is divided into multiple layers, the number of which matches the number of layers in the drying mounting component 40140. Each layer of heating element 40160 corresponds to a set of independent blowing air outlets, allowing for individual adjustment of the hot air volume and temperature of each layer to adapt to the drying needs of consumables with different layer heights. The blowing component 40170 is upgraded to a variable frequency centrifugal fan, which precisely controls the air volume by adjusting the fan speed to avoid excessive air velocity causing sample liquid to splash.

[0353] In some embodiments, a vent is provided at the bottom of the slide 40141 of the drying mounting component 40140, and an auxiliary blowing port is added to the side wall of the drying box 40100. Hot air is blown from the vent to the bottom of the consumables to eliminate the dead zone of airflow at the bottom and ensure that the drying rate of the upper and lower surfaces of the tray is consistent.

[0354] In some embodiments, the heating element 40160 is a sealed stainless steel heating tube with an anti-stick coating to prevent sample residue from adhering. A high-temperature resistant metal protective mesh is installed on the outside of the heating tube, which does not affect heat transfer and prevents foreign objects from contacting the heating tube. At the same time, an overheat fuse is installed next to the heating element 40160. The temperature threshold can be set. If the heating tube overheats locally, the fuse will automatically disconnect to eliminate the risk of fire.

[0355] In some embodiments, for drying biological samples containing organic reagents, an activated carbon filter module and an explosion-proof pressure relief valve are installed at the air outlet of the drying chamber 40100 to filter the volatilized organic waste gas. The pressure relief valve automatically opens to release pressure when the air pressure inside the chamber is abnormal. The heating element 40160 uses an explosion-proof heating tube, which is suitable for drying flammable and explosive samples.

[0356] In some embodiments, multiple PT100 high-precision temperature sensors are installed at different locations inside the drying oven 40100. The sensor signals are fed back to the PLC control system to monitor the temperature inside the oven in real time. The control system automatically adjusts the power of the heating element 40160 and the air speed of the blowing element 40170 according to the preset drying curve to achieve precise temperature control.

[0357] See Figure 10 The drying oven 40100 is also equipped with a temperature sensing element 40150, which is communicatively connected to the heating element 40160; the inner wall of the drying oven 40100 is provided with a heat insulation layer 40130.

[0358] In some embodiments, a temperature sensing element 40150 is disposed inside the drying oven 40100 and is signal-connected to the heating element 40160. It is used to collect environmental parameters inside the oven and feed them back to the control terminal of the heating element 40160. The heating power is adjusted as needed to provide the heat energy required for drying and to prevent temperature runaway. An insulation layer 40130 is attached to the inner wall of the drying oven 40100 to reduce heat loss from the oven, lower the heat compensation frequency of the heating element 40160, maintain a stable temperature inside the oven, and reduce energy consumption.

[0359] In some embodiments, the insulation layer 40130 adopts a multi-layer composite insulation structure, upgraded to a three-layer composite structure. The inner layer uses a high-temperature resistant antibacterial coating to prevent the adhesion of volatile substances from the sample, is easy to clean, and can withstand high temperatures of 150°C without deformation. The middle layer uses high-density aluminum silicate insulation cotton, which is the core insulation layer 40130, with a thickness of 20mm-30mm, adapted to the volume of the drying oven 40100. The outer layer uses an aluminum foil reflective layer to reflect infrared heat radiation inside the oven, further reducing heat loss. The three-layer structure is tightly bonded to the inner wall of the drying oven 40100 with a high-temperature resistant adhesive, with no air gaps, increasing the insulation efficiency to over 90%.

[0360] See Figure 1 In some embodiments, the multifunctional sample reaction plate preparation platform further includes a stacking device 8000, which is used to store consumables and to interact with the consumables in the sample transfer module 10000 and the reagent addition module 20000; the consumables include sample plate 0001 and reaction plate 0002.

[0361] The stacking device 8000 in the sample transfer module 10000 of this application embodiment has a large storage capacity for consumables, reducing the frequency of replenishment and avoiding process interruption; through automated interactive picking and placing of consumables, it eliminates reliance on manual labor, solves the positioning deviation problem caused by manual operation, improves the efficiency of consumable loading and plate making, and at the same time ensures the accuracy of pipetting.

[0362] In some embodiments, the stacking device 8000 is used to interact with the sample transfer station to exchange sample plate 0001 and reaction plate 0002, so as to provide sample plate 0001 and reaction plate 0002 to the sample transfer station; the stacking device 8000 is used to interact with the reagent addition station 20011 to exchange reaction plate 0002, so as to recover reaction plate 0002.

[0363] In some embodiments, the stacking device 8000 is at least partially connected to the drying module 40000 for interacting with the drying preparation station to receive the reaction plate 0002.

[0364] In some embodiments, the stacking device 8000 is at least partially connected to the heat sealing station for interacting with the reaction plate 0002 at the heat sealing station to receive the reaction plate 0002.

[0365] In some embodiments, the stacking device 8000 can be located at the inlet and / or outlet of the logistics transfer interaction module 6000 to provide reagent plates, sample plates 0001, and reaction plates 0002 to various functional modules via the logistics transfer interaction module 6000. Specifically, the stacking device 8000 can adopt an integrated structure, storing reagent plates, sample plates 0001, and reaction plates 0002 separately in mutually isolated areas. Alternatively, the stacking device 8000 can adopt a split structure, such as using multiple stacking cages to store reagent plates, sample plates 0001, and reaction plates 0002 separately. The reagent plates, sample plates 0001, and reaction plates 0002 can be grasped by a robotic arm using gripping or adsorption and placed into the logistics transfer interaction module 6000 for transmission to the respective functional modules. Optionally, to distinguish between the reagent plates, sample plates 0001, and reaction plates 0002, corresponding identification marks, such as QR codes or barcodes, can be set on them. In practice, the reagent plate, sample plate 0001, and reaction plate 0002 can be scanned for confirmation when the robotic arm picks up the reagent plate, sample plate 0001, and reaction plate 0002, or when the reagent plate, sample plate 0001, and reaction plate 0002 enter the next functional module. If the scan is successful, the subsequent process will proceed; otherwise, an error will be reported and the user will need to confirm and process it.

[0366] In some embodiments, the stacking device 8000 includes a hierarchical stacking module 8100 and a stacked stacking module 8200. The hierarchical stacking module 8100 includes a plurality of independent storage spaces and is configured to independently access consumables in any of the independent storage spaces. The consumables in the hierarchical stacking module 8100 include a sample plate 0001. The stacked stacking module 8200 includes a stacked storage space 82111 and is configured to sequentially access consumables in the stacked storage space 82111 in a first-in-first-out order. The consumables in the stacked stacking module 8200 include a reaction plate 0002.

[0367] Specifically, the hierarchical stack module 8100's several independent storage spaces are independent of each other and do not interfere with each other, enabling independent access. Consumables can be directly accessed and placed in any independent storage space without following a fixed order, without moving consumables in other spaces. This module is compatible with the stack of sample board 0001. Because the sample information carried within sample board 0001 varies, specific sample board 0001 needs to be flexibly retrieved according to requirements during experiments, without following a fixed order. The independent access capability allows direct location and placement of the target sample board 0001 without moving other consumables.

[0368] The stacked storage space 82111 of the stacked stacking module 8200 is accessed in a first-in, first-out (FIFO) order, meaning consumables are retrieved and placed in the order they are inserted. This is suitable for consumables like reaction plates 0002 that can be processed in batches. Because reaction plates 0002 are produced in high-throughput batches, the large batches of reaction plates are indistinguishable, with identical channel specifications and materials, and no unique sample information. Therefore, the order of retrieval and placement does not affect the experimental results; FIFO retrieval is sufficient without additional precise positioning operations.

[0369] Through the above configuration, the stacking device 8000 uses a combination of layered and stacked stacking to precisely match the characteristics of consumables. For the differentiated information requirements of sample plates 0001, independent access ensures retrieval flexibility and avoids the impact of sample plate 0001 confusion on experimental results. For the undifferentiated characteristics of reaction plates 0002, sequential access simplifies the operation process, eliminating the need for complex positioning mechanisms. Sample plates 0001 do not need to be searched in a fixed order; the target consumable can be retrieved directly, reducing unnecessary actions. Reaction plates 0002 are stacked in batches and retrieved sequentially, increasing storage density while accelerating retrieval speed and reducing process interruptions during high-throughput plate making. The flexible retrieval of sample plates 0001 can quickly respond to different liquid aspiration needs of the sample pipetting head module 1000, while the sequential retrieval of reaction plates 0002 can match the batch spraying rhythm. Both prevent the transfer and pipetting progress from becoming disconnected, improving the overall plate making efficiency of the system.

[0370] See Figure 15 and Figure 16 In some embodiments, the hierarchical stack module 8100 includes a hierarchical storage unit 8110, a position identification unit 8120, and a stack handling unit 8130. The hierarchical storage unit 8110 has several layers of independent storage spaces arranged vertically for storing consumables in layers. The position identification unit 8120 is used to acquire the identification information and first position information of the consumables, as well as the type information of the hierarchical storage unit 8110. The stack handling unit 8130 is communicatively connected to the position detection unit and is used to retrieve consumables from the corresponding independent storage space based on the first position information, or to place consumables back into the corresponding independent storage space.

[0371] Specifically, the hierarchical storage unit 8110 divides the vertical space into several independent storage spaces, giving each sample plate 0001 an independent storage location. These independent storage spaces do not interfere with each other, ensuring the independence of sample plate 0001 storage while efficiently utilizing vertical space to increase storage density. The position identification unit 8120 collects the identification information of consumables, such as barcodes to distinguish different samples and the first position information corresponding to the consumable, i.e., the coordinates of its independent storage space. It can also collect the type information of the hierarchical storage unit 8110, providing support for accurate retrieval and placement. The stacking and handling unit 8130 communicates with the position identification unit 8120, receiving the first position information transmitted by it, and then accurately locates the independent storage space where the target consumable is located to complete the plate retrieval or placement operation. The entire process does not rely on a fixed order, nor does it require moving consumables in other independent storage spaces. The barcode information includes, but is not limited to, the sample plate 0001 number, sample type, sample plate 0001 type, number of replicated samples, number of replicates, reaction plate 0002 type, reagent information, and reaction plate 0002 number.

[0372] Through the above settings, precise traceability and retrieval of consumables are achieved. The position identification unit 8120 binds the identification information of the consumables with the first position information. The stacking device 8000 can automatically find the storage coordinates of the consumables based on the barcode information identified by the position identification unit 8120 and automatically complete the output action of the consumables, enabling rapid positioning of any target consumable. The vertically arranged independent storage space maximizes the use of vertical space, increases the number of consumables stored within a limited area, reduces the frequency of replenishment during high-throughput experiments, avoids process interruptions, and improves storage efficiency and space utilization. The stacking and handling unit 8130 automatically performs pick-and-place actions according to the first position information without manual intervention, which reduces labor intensity and avoids deviations caused by manual positioning, ensuring the accuracy of docking with the sample pipetting head module 1000 and improving pipetting accuracy.

[0373] Furthermore, the in-situ detection unit acquires the type information of the hierarchical storage unit 8110, which can adapt to the storage needs of consumables of different specifications. It can meet diverse experimental scenarios without changing modules, thus improving system flexibility. Different types of hierarchical storage units 8110 may have different layer heights and number of layers, but the height of the first layer is the same. The layer height and number of layers parameters of the stacking cage type are stored in the database. The size parameters of the hierarchical storage unit 8110 can be retrieved by scanning the barcode of the hierarchical storage unit 8110.

[0374] In some embodiments, the hierarchical stack module 8100 further includes a hierarchical frame 8140, on which the hierarchical storage unit 8110 and the stack handling unit 8130 are mounted or connected. To support the stable operation of each unit, the overall hierarchical frame 8140 needs to have a certain rigidity, and the weight of the whole machine is required to be less than or equal to 40kg. Considering manufacturing costs, a solution of bending sheet metal and riveting it together to form a riveted frame is adopted, and an interactive panel is mounted on the riveted frame.

[0375] See Figure 16 In some embodiments, the stack handling unit 8130 includes a layered handling unit 8131, a layered rotating assembly 8132, and a layered telescopic pick-and-place assembly 8133. The layered handling unit 8131 is used to provide lifting driving force; the layered rotating assembly 8132 is mounted on the layered handling unit 8131 and is used to provide rotational movement about a preset rotation axis; multiple layered storage units 8110 are provided and arranged circumferentially along the preset rotation axis; the layered telescopic pick-and-place assembly 8133 is mounted on the layered rotating assembly 8132 and is used to provide telescopic movement in the horizontal direction to pick up and place consumables in independent storage spaces.

[0376] Specifically, the layered transport unit 8131 provides vertical driving force, adapting to the vertical layered structure of the layered storage unit 8110. Based on the layer height information of the target consumable, it drives the entire stack transport unit 8130 to rise and fall to the corresponding height. The layered rotation component 8132 is installed on the layered transport unit 8131, providing rotational movement around a preset rotation axis. The layered storage units 8110 are arranged circumferentially along this rotation axis. By rotating, the layered rotation component 8132 can drive the subsequent layered telescopic pick-and-place component 8133 to align with the independent storage spaces in different directions, effectively covering all storage locations. The layered telescopic pick-and-place component 8133 is installed on the layered rotation component 8132, providing telescopic movement in the horizontal direction. When the layered transport unit 8131 is positioned at the target layer height and the layered rotation component 8132 is aligned with the target storage location, the layered telescopic pick-and-place component 8133 extends to pick up and place the consumable in the corresponding independent storage space. After completing the action, it retracts and resets.

[0377] Through the above configuration, the coordinated operation of the layered handling unit 8131, the layered rotating component 8132, and the layered telescopic pick-and-place component 8133 can cover all independent storage spaces of the layered storage unit 8110, meeting the need for flexible retrieval of consumables and avoiding access failures or consumable damage caused by inaccurate positioning. The circumferential arrangement of the layered storage unit 8110, in conjunction with the layered rotating component 8132, maximizes the storage capacity within a limited footprint, while ensuring smooth pick-and-place operations, reducing unnecessary travel, significantly shortening single access time, and improving the continuity of high-throughput experiments. No manual intervention is required throughout the process; the standardized operation process reduces labor intensity and avoids deviations caused by manual operation. This structure can adapt to storage units with different numbers of layers and different circumferential distributions, and can accommodate the storage needs of various consumable specifications without significant modifications. The stack handling unit 8130 is communicatively connected to the sample pipetting head module 1000, which allows the action rhythm of the stack handling unit 8130 to match the pipetting progress of the sample pipetting head module 1000, avoiding the disconnect between the storage and retrieval actions and the pipetting process, and ensuring the overall plate-making efficiency of the system.

[0378] In some embodiments, the layered transport unit 8131 includes a layered lifting motor 81311, lifting synchronous pulleys 81312, and a lifting synchronous belt 81313. The layered lifting motor 81311 is configured as a stepper motor, driving the lifting pulleys to rotate, thereby driving the lifting synchronous belt 81313 to rotate. A lifting mounting plate 81313a is mounted on the lifting synchronous belt 81313, and the layered rotating assembly 8132 is mounted on the lifting mounting plate 81313a. The layered transport unit 8131 also includes a lifting guide rail 81314, on which the lifting mounting plate 81313a is slidably connected.

[0379] In some embodiments, the layered transport unit 8131 is provided with a counterweight mechanism 81316, which is connected to the load end of the layered transport unit 8131, namely the lifting mounting plate 81313a, to balance the load of the layered transport unit 8131. The layered transport unit 8131 needs to drive the layered rotating assembly 8132, the layered telescopic picking and placing assembly 8133, and the sample plate 0001 to be picked up and placed to move together. The combined load of these components increases the pressure of the lifting drive, while the counterweight mechanism 81316 offsets part of the load with its own weight, making the force on the layered transport unit 8131 more balanced.

[0380] Through the above configuration, the counterweight mechanism 81316 reduces drive energy consumption and losses, avoids lifting and lowering jams and shaking caused by uneven force, ensures the accuracy of the stacking and handling unit 8130 in longitudinal positioning, and indirectly improves the stability of sample board 0001 picking and placing. At the same time, with the load reduced, the start, stop and speed adjustment response of the layered handling unit 8131 is more sensitive, shortening the action time. Together with the layered rotating component 8132 and the layered telescopic picking and placing component 8133, it improves the overall storage and retrieval efficiency. In addition, the balanced force reduces the deformation risk of the layered handling unit 8131, avoids structural loosening caused by long-term heavy load, and ensures the stability of the layered stacking module 8100 during long-term high-frequency operation.

[0381] Specifically, the counterweight mechanism 81316 includes a pulley structure 81316a and a wire rope 81316b mounted on the lifting frame 81315. The wire rope 81316b is connected to the pulley structure 81316a. One end of the wire rope 81316b is connected to the counterweight block 81316c, and the other end is connected to the lifting mounting plate 81313a. By changing the direction of force through the pulley structure 81316a, the weight of the counterweight block 81316c is balanced with the load weight of the lifting mounting plate 81313a, thus achieving power-off hovering.

[0382] Through the above configuration, the cooperation between the pulley structure 81316a and the wire rope 81316b ensures that the tension of the counterweight 81316c is evenly transmitted to the lifting mounting plate 81313a, effectively reducing the tension on the synchronous belt and extending the service life of the lifting synchronous belt 81313. The compact structural design does not occupy additional lateral space and is suitable for the compact layout requirements of integrated equipment. The optimized force balance and power-off stability of the layered handling unit 8131 solves the problems of unreliable suspension and excessive tension of the synchronous belt in traditional counterweight structures, while also preventing mechanical damage caused by falling during power outages.

[0383] See Figure 17 In some embodiments, the layered rotating assembly 8132 includes a rotary motor 81321, a rotary drive gear 81322, and a rotary driven gear 81323. The rotary motor 81321 is configured as a stepper motor with an encoder and is directly connected to the rotary drive gear 81322. The rotary drive gear 81322 meshes with the rotary driven gear 81323, and the rotary driven gear 81323 is connected to the layered telescopic pick-and-place assembly 8133.

[0384] See Figure 18In some embodiments, the layered telescopic pick-and-place assembly 8133 includes a pick-and-place frame 81331 and a pick-and-place tray 81332, a pick-and-place motor 81333, a pick-and-place synchronous pulley, a pick-and-place synchronous belt, and a pick-and-place guide rail 81334 disposed on the pick-and-place frame 81331. The pick-and-place motor 81333 drives the pick-and-place synchronous pulley to rotate, and the pick-and-place synchronous pulley drives the pick-and-place synchronous belt to rotate. The pick-and-place tray 81332 is fixedly connected to the pick-and-place synchronous belt and slidably connected to the pick-and-place guide rail 81334. Through the above arrangement, the pick-and-place motor 81333 drives the pick-and-place tray 81332 to telescopically move in the horizontal direction.

[0385] In some embodiments, the layered telescopic take-up and drop assembly 8133 is provided with an in-situ detection unit 81335 for detecting whether consumables exist in the independent storage space on the layered telescopic take-up and drop assembly 8133 and at the corresponding position.

[0386] Specifically, the in-situ detection unit 81335 is integrated on the layered telescopic pick-and-place component 8133. On the one hand, it detects whether the layered telescopic pick-and-place component 8133 itself has carried consumables, such as confirming whether the consumables have been successfully picked up after picking up the board, and confirming whether the component is empty after placing the board. On the other hand, it detects whether there are consumables in the target independent storage space, such as confirming that there are consumables to be retrieved at this location before picking up the board.

[0387] The above settings prevent accidental operation and damage to consumables, avoid performing a board retrieval operation when there are no consumables in the target space, and avoid performing a board placement operation when there are already consumables in the target space. This also prevents sample board 0001 from being damaged by collision or compression, ensuring the structural safety of consumables and the layered storage unit 8110. The detection results provide a clear signal for starting and stopping the operation of the layered telescopic pick-and-place component 8133, preventing process interruptions due to failed grabbing or misplacement. No manual confirmation of consumable status is required; the entire process of detection, judgment, and action execution is automated, reducing human intervention points, lowering operational complexity, adapting to the continuous operation requirements of high-throughput experiments, and improving the overall board manufacturing process continuity.

[0388] It is understandable that the height of the layered handling unit 8131 is relatively high, and its perpendicularity to the table surface is greatly affected by assembly and processing errors. In order to reduce the processing difficulty, an adjustment device is designed to make the layered telescopic picking and placing component 8133 flush with and centered with the target sample plate 0001.

[0389] See Figure 19In some embodiments, the stackable module 8200 includes a stackable storage unit 8210, a stackable transfer unit 8220, and a stackable lifting unit 8230. The stackable storage unit 8210 is provided with a stackable storage space 82111 for stacking multiple consumables in a vertical direction. The stackable transfer unit 8220 is used to reciprocate horizontally below the stackable storage unit 8210 to feed or remove consumables into or from the stackable storage unit 8210. The stackable lifting unit 8230 is located below the stackable transfer unit 8220 and is used to move vertically to cooperate with the stackable storage unit 8210 to release the bottommost consumable in the stackable storage unit 8210 onto the stackable transfer unit 8220, or to lift and store the consumables on the stackable transfer unit 8220 into the stackable storage space 82111.

[0390] Specifically, the stacking storage unit 8210 constructs a stacking storage space 82111 along the vertical direction, stacking multiple reaction plates 0002 layer by layer to maximize the use of vertical space and increase the amount of consumables stored per unit area. The stacking transfer unit 8220 is located below the stacking storage unit 8210 and can move back and forth in the horizontal direction to remove the reaction plates 0002 from the stacking storage unit 8210 and transfer them to the system interaction position, or to transfer the reaction plates 0002 to be stored to a preset position below the stacking storage unit 8210, realizing the horizontal transfer of consumables. The stacking lifting unit 8230 is located below the stacking transfer unit 8220 and can move up and down vertically. It works in conjunction with the stacking storage unit 8210. When picking up a plate, the stacking lifting unit 8230 rises to receive the reaction plate 0002 at the bottom of the stacking storage unit 8210. After the stacking storage unit 8210 releases the consumables, the stacking lifting unit 8230 descends to place the reaction plate 0002 on the stacking transfer unit 8220. When placing a plate, the stacking lifting unit 8230 rises to lift the reaction plate 0002 on the stacking transfer unit 8220 and send it into the stacking storage space 82111 to complete the storage.

[0391] Through the above settings, vertical stacking increases the number of reaction plates 0002 stored in a single stacking storage unit 8210, reducing the frequency of material replenishment during high-throughput plate making, avoiding process interruptions, and adapting to the batch processing needs of large-scale experiments. Manual loading and unloading of each plate is eliminated; the automated picking, placing, and transferring of reaction plates 0002 is achieved through the coordinated action of the stacking module 8200, freeing users from manual labor, reducing labor intensity, and avoiding positioning errors caused by manual operation. The vertical stacking of reaction plates 0002 combined with horizontal transfer ensures standardized and continuous picking and placing operations with short single-time storage and retrieval times, matching the pace requirements of high-throughput plate making and improving overall process efficiency. The horizontal transfer direction of the stacking transfer unit 8220 can be interfaced with the interaction position of the logistics transmission interaction module 6000 or the sample pipetting head module 1000. The lifting rhythm of the stacking lifting unit 8230 can be matched with the actions of the stacking transfer unit 8220 and the stacking storage unit 8210, ensuring the overall plate making efficiency of the system.

[0392] In some embodiments, the stackable module 8200 further includes a stacking frame 8240, and the stacking storage unit 8210, the stacking transfer unit 8220, and the stacking lifting unit 8230 are all mounted or connected to the stacking frame 8240.

[0393] In some embodiments, the stacking storage unit 8210 includes a stacking cage 8211, which is provided with a stacking interface. The stacking interface can be configured as a pin and screw hole. The accurate recovery and removal of the reaction plate 0002 can be achieved by positioning and cooperating with the corresponding position of the stacking transfer unit 8220 through the pin.

[0394] Specifically, in some embodiments, the limiting structure of the stacking cage 8211 uses eight cylindrical rods, with two cylindrical rods at each of the four corners, corresponding to the four right-angled sides of the reaction plate 0002, forming a surrounding limiting boundary. The cylindrical rods extend vertically, collectively forming the limiting frame of the stacking storage space 82111, ensuring that the reaction plate 0002 is always in a preset position when stacked vertically.

[0395] See Figure 20 In some embodiments, the stacked storage unit 8210 includes a latching mechanism 8212 disposed at the bottom of the stacked storage space 82111. The latching mechanism 8212 includes a rotatable latching member 82121 and a latching drive assembly 82122 drivenly connected to the latching member 82121. The latching member 82121 is used to abut against consumables at the bottom of the stacked storage space 82111 and store them inside the stacked storage space 82111. The latching drive assembly 82122 drives the latching member 82121 to rotate, so as to drive the latching member 82121 to abut against or release consumables.

[0396] Specifically, the latching component 82121 is a rotatable structure used to abut against the bottommost reaction plate 0002 within the stacked storage space 82111. Through mechanical limiting, all stacked reaction plates 0002 are stably stored within the space, preventing consumables from falling out. The latching drive assembly 82122 provides the power for the rotation of the latching component 82121. When it is necessary to store the reaction plate 0002, the latching component 82121 is driven to rotate to the abutment position, cooperating with the stacking lifting unit 8230 to firmly fix the reaction plate 0002 within the stacking space. When it is necessary to release the reaction plate 0002, the latching component 82121 is driven to rotate in the opposite direction to disengage from the abutment, allowing the bottommost reaction plate 0002 to smoothly fall into the stacking transfer unit 8220 along with the stacking lifting unit 8230, achieving orderly release.

[0397] With the above configuration, the latching mechanism 8212 is synchronized with the stacking lifting unit 8230 and the stacking transfer unit 8220 through system control, enabling it to complete storage or release without manual intervention, thus meeting the continuous operation requirements of high-throughput plate manufacturing. Furthermore, the mechanical latching structure is simple and can accommodate SBS standard reaction plates 0002 of varying thicknesses, satisfying diverse batch storage needs without structural adjustments.

[0398] In some embodiments, the latching drive assembly 82122 includes an electromagnetic drive component 82122a and a latching reset component 82122b. The electromagnetic drive component 82122a is driven to the latching component 82121 and is used to drive the latching component 82121 to rotate when energized. The latching reset component 82122b is driven to the latching component 82121 and is used to provide a reset driving force for the latching component 82121 after the electromagnetic drive component 82122a is de-energized.

[0399] In some embodiments, rotatable latching members 82121 are symmetrically arranged in pairs at the bottom of the stacked storage unit 8210. The front end spacing of their respective parts is designed to be 82.8 mm, which matches the 82.4 mm spacing between the top two ends of the reaction plate 0002 and the 85 mm skirt width. This mechanical limitation enables the stacked reaction plates 0002 to be supported and isolated in layers. The electromagnetic drive 82122a is configured as a telescopic electromagnet, which serves as a power source and is connected to the latching members 82121 via a latching link 82122c. When the bottom reaction plate 0002 needs to be released, the electromagnetic drive 82122a is energized to generate magnetic attraction. The internal magnetic column pulls the latching link 82122c to rotate, thereby causing the rotatable latching members 82121 on both sides to rotate synchronously. This causes the front end of the latching member 82121 to disengage from the skirt of the upper reaction plate 0002, releasing the limitation on the lower reaction plate 0002 and releasing the reaction plate 0002. When the reaction plate 0002 descends to the preset distance, the electromagnetic drive component 82122a is de-energized, and the magnetic attraction disappears. The latch reset component 82122b is configured as a torsion spring. The torsion spring releases the pre-stored elastic potential energy, driving the latch linkage 82122c to rotate in the opposite direction, causing the latch component 82121 to spring back and reset. At this time, the front end of the latch component 82121 is re-embedded between the upper and lower reaction plates 0002, and the bottom reaction plate 0002 can fall smoothly into the stacking transfer unit 8220, while the upper reaction plate 0002 is supported by the latch component 82121, maintaining the stacked state.

[0400] With the above settings, the electromagnetic drive responds quickly and can drive the latching component 82121 to rotate rapidly; the latching reset component 82122b ensures that the latching component 82121 is stably reset after power failure, avoiding the latching component 82121 from jamming due to power interruption; the electromagnetic drive structure is simple and small in size, and is suitable for the bottom installation space of the stacked storage unit 8210.

[0401] See Figure 21 In some embodiments, the stacking and transfer unit 8220 includes a transfer frame 8221 and a transfer component 8222. The transfer component 8222 carries consumables and extends horizontally, and is movably mounted on the transfer frame 8221 in the horizontal direction. The stacking and transfer unit 8220 also includes a transfer motor 8225, a transfer drive wheel 8223, and a transfer driven wheel 8224. The transfer drive wheel 8223 and the transfer driven wheel 8224 are spaced apart horizontally on the transfer frame 8221. The transfer component 8222 includes a transfer conveyor belt, which is rotatably fitted onto the transfer drive wheel 8223 and the transfer driven wheel 8224. With this design, the transfer conveyor belt is driven to rotate by the transfer drive wheel 8223 and the transfer driven wheel 8224, resulting in a simple structure and high reliability. The transfer conveyor belt can be a flat belt, a V-belt, or other types of belt.

[0402] With the above configuration, consumables are placed on the transfer member 8222 of the stacking and transfer unit 8220. The transfer member 8222 carries the consumables, and by moving the transfer member 8222 horizontally, it can move the consumables horizontally as well. The transfer member 8222 can reciprocate horizontally to move the consumables. In other embodiments, the transfer member 8222 can move only horizontally, as long as it can move the consumables. Furthermore, the transfer member 8222 can move forward or backward in the horizontal direction, depending on the actual usage scenario.

[0403] In some embodiments, the stacking transfer unit 8220 further includes a diffuse reflection sensor 8226 disposed on the transfer frame 8221. When multiple stacking storage units 8210 are arranged horizontally, the transfer frame 8221 is also arranged horizontally with diffuse reflection sensors 8226 at corresponding positions and in corresponding numbers to the stacking storage units 8210. Each diffuse reflection sensor 8226 is used to detect the presence status of the reaction plate 0002 in the transfer unit to determine whether the plate has been successfully retrieved or delivered.

[0404] In some embodiments, the stacking lifting unit 8230 includes a stacking lifting tray 8231 and a stacking lifting drive assembly 8232. Along the direction of feeding consumables from the stacking transfer unit 8220 to the stacking storage unit 8210, the stacking lifting tray 8231 is provided with a first side 82311 and a second side 82312 in sequence, and the top height of the second side 82312 is higher than the top height of the first side 82311. The stacking lifting drive assembly 8232 is drivenly connected to the stacking lifting tray 8231 and is used to drive the stacking lifting tray 8231 to rise and fall to the board picking station and the positioning station. In the board picking station, the first stop 82311 and the second stop 82312 together abut against the bottommost consumable in the stacked storage unit 8210 to support the consumable; in the positioning station, the second stop 82312 is used to stop and limit the horizontal movement of the consumable on the stacked transfer unit 8220, while the first stop 82311 does not interfere with the horizontal movement of the consumable, so that the consumable is positioned at the preset interactive position for feeding the consumable into the stacked storage unit 8210.

[0405] Specifically, at the board retrieval station, the stacking lifting tray 8231 rises to a height suitable for the bottom of the stacked storage unit 8210. The first edge 82311 and the second edge 82312 together abut against the reaction plate 0002 at the bottom of the stacked storage unit 8210, forming a stable support. When the latching mechanism 8212 releases the consumables, the stacking lifting tray 8231 drives the reaction plate 0002 to descend smoothly, transferring it to the stacking transfer unit 8220 below. At the positioning station, the stacking lifting tray 8231 rises to a height suitable for the stacking transfer unit 8220. The second edge 82312 limits the reaction plate 0002 on the stacking transfer unit 8220 by its own height, preventing it from moving horizontally. At the same time, the first edge 82311, due to its lower height, does not interfere with the movement of the consumables, allowing the reaction plate 0002 to be accurately positioned at the preset interactive position in the stacked storage unit 8210, facilitating subsequent lifting and storage.

[0406] With the above settings, at the board retrieval station, the first and second guard edges 82311 and 82312 jointly support the reaction board 0002, preventing the consumable from tilting or falling due to uneven force on a single guard edge. Combined with the release action of the latching mechanism 8212, this ensures the smooth transfer of the bottom reaction board 0002 to the stacking transfer unit 8220. At the positioning station, the second guard edge 82312 precisely stops and limits the movement, while the first guard edge 82311 does not interfere with the transfer, ensuring that the reaction board 0002 is in a preset interactive position before being sent into the stacking storage unit 8210. This avoids storage failure or consumable collision due to positioning deviations, ensuring accurate docking with the stacking storage unit 8210.

[0407] See Figure 23 In some embodiments, the stacking and lifting unit 8230 further includes a stacking and lifting guide assembly 8233, which includes two vertically arranged lifting guide columns, and a lifting plate is slidably connected to the lifting guide columns.

[0408] In the stacking stack module 8200 of the stacking device 8000 provided in this application embodiment, multiple stacking stack modes can be set, including but not limited to the following modes:

[0409] Mode 1: It consists of two stacked storage units 8210 for loading. Both stacked storage units 8210 serve as batch supply units for reaction plates 0002, and output the reaction plates 0002 to be processed to the system in a first-in-first-out order.

[0410] Mode 2: It consists of two stacked storage units 8210 for recycling. Both stacked storage units 8210 serve as batch storage units for reaction plates 0002, storing the processed reaction plates 0002 in a first-in-first-out order.

[0411] Mode 3: It consists of a stacked storage unit 8210 for loading and a stacked storage unit 8210 for recycling. The stacked storage unit 8210 for loading outputs the reaction plate 0002 to be processed, and the stacked storage unit 8210 for recycling simultaneously collects the corresponding processed reaction plate 0002.

[0412] It is understandable that the stack device 8000 can be flexibly configured according to specific usage requirements and is not limited to the three modes mentioned above.

[0413] In some embodiments, the logistics transmission interaction module 6000 further includes a sample plate conveying mechanism 6100 and a reaction plate conveying mechanism 6200. The sample plate conveying mechanism 6100 is used to interact with the layered stacking module 8100 to convey the sample plate 0001 to the sample plate 0001 liquid absorption position. The reaction plate conveying mechanism 6200 is used to interact with the stacked stacking module 8200 to convey the reaction plate 0002 to the reaction plate 0002 liquid spraying position or the next station.

[0414] Specifically, the sample plate conveying mechanism 6100 interacts with the hierarchical stacking module 8100 to receive the independently stored sample plate 0001. When the hierarchical stacking module 8100 retrieves the target sample plate 0001 according to experimental requirements, the sample plate conveying mechanism 6100 interacts with the module to receive the sample plate 0001 and transfer it to the liquid aspiration position of the sample plate 0001, providing consumables for the liquid aspiration operation of the sample pipetting head module 1000. At the same time, the used sample plate 0001 can be recycled to the corresponding independent storage space of the hierarchical stacking module 8100, or recycled to the stacked storage space 82111 of the stacked stacking module 8200.

[0415] The reaction plate conveying mechanism 6200 interacts with the stacked module 8200 for consumables. After the stacked module 8200 releases the reaction plates 0002 in sequence, the reaction plate conveying mechanism 6200 receives the reaction plates 0002 and transfers them to the liquid spraying position of the reaction plate 0002 for the sample pipetting head module 1000 to complete the liquid spraying operation. After the liquid spraying is completed, the reaction plate 0002 can continue to be transferred to the next station, such as the heat sealing station or the drying station, or recycled to the stacking storage space 82111 of the stacked module 8200.

[0416] Through the above setup, the logistics transmission interaction module 6000 and the stacking device 8000 are used in conjunction to replace the manual transfer of consumables, realizing the automated connection of sample plate 0001 and reaction plate 0002 from the stacking device 8000 to the liquid transfer station. This avoids process interruptions caused by manual handling, solves the problem of low loading efficiency on existing equipment, and adapts to the continuous operation requirements of high-throughput plate production. The conveying mechanism moves along a preset trajectory with high positioning accuracy, accurately delivering sample plate 0001 and reaction plate 0002 to the corresponding liquid aspiration and spraying stations, avoiding the impact of consumable positioning deviations on liquid transfer accuracy, and solving the positioning error problem caused by manual operation.

[0417] See Figure 22 and Figure 23 In some embodiments, the stacking transfer unit 8220 includes a stack docking structure. Several stacking transfer units 8220 can be arranged horizontally and connected in sequence. Adjacent stacking transfer units 8220 can dock through the stack docking structure, or they can dock with the logistics transmission interaction module 6000 through the stack docking structure.

[0418] In some embodiments, the stack docking structure includes a stack docking post 8227 and a stack docking hole 8228 that fit together. The stack docking post 8227 and the stack docking hole 8228 are respectively disposed on two adjacent stacking transfer units 8220; or one is disposed on a stacking transfer unit 8220 and the other is disposed on an adjacent logistics transmission interaction module 6000. The above-described stack docking structure is simple and easy to operate. Each stacking transfer unit 8220 has a stack docking post 8227 and a stack docking hole 8228 at both ends, allowing any stacking transfer unit 8220 to successfully dock during the docking process.

[0419] In other embodiments, other forms of stack docking structures may also be used. For example, a magnetically engaged stack docking structure.

[0420] In some embodiments, the stacking transfer unit 8220 further includes a stacking fixing seat 82211. The transfer frame 8221 has stacking fixing seats 82211 at both ends along the horizontal direction. Stacking docking posts 8227 and stacking docking holes 8228 are respectively disposed at the stacking fixing seats 82211 at both ends of the transfer frame 8221. By adjusting the relative positions of the stacking fixing seats 82211 and the transfer frame 8221, the relative positions of the stacking docking structure and the transfer frame 8221 can be adjusted, thereby improving the success rate of alignment between the two stacking transfer units 8220 and increasing alignment efficiency.

[0421] See Figures 27 to 31In some embodiments, the sample pipetting mechanism 1100 includes a piston cavity assembly 1110 and a piston shaft assembly 1120. The piston cavity assembly includes a plurality of pipette tip connecting portions 1112, and the piston shaft assembly 1120 is slidably connected to the piston cavity assembly 1110. The sample aspiration and dispensing driving mechanism 1200 drives the piston shaft assembly 1120 to reciprocate and slide to provide positive or negative pressure to the pipette tip connecting portions 1112. The piston shaft assembly 1120 includes a piston shaft pressure plate 1121 and a piston shaft pressure plate 1122. The piston shaft 1122 and the flexible fixing assembly 1123, the piston shaft pressure plate 1121 are used to connect to the pipette tip quick change device 1300, and can be detachably connected to the sample aspiration and spraying drive mechanism 1200 through the pipette tip quick change device 1300; the piston shaft 1122 is slidably connected to the piston cavity assembly 1110; the flexible fixing assembly 1123 flexibly connects the piston shaft 1122 to the piston shaft pressure plate 1121, and the piston shaft 1122 slides back and forth with the piston shaft pressure plate 1121.

[0422] Specifically, in actual production and assembly processes, slight deviations in the machining accuracy and installation position of the piston shaft pressure plate 1121 and the piston shaft 1122 are inevitable. If a rigid connection is used, these errors will cause the piston shaft 1122's movement trajectory to deviate, or even lead to jamming or sealing failure. The piston shaft 1122 achieves a flexible connection with the piston shaft pressure plate 1121 through a flexible fixing component 1123. This flexible connection can compensate for these errors through its own deformation or gaps, ensuring that the piston shaft 1122 always slides along a preset trajectory. Simultaneously, eliminating assembly errors ensures the sealing and smooth movement between the piston shaft 1122 and the piston cavity assembly 1110, avoiding air pressure leakage or uneven movement resistance caused by shaft misalignment, thereby stabilizing the accuracy of the liquid volume for aspiration and dispensing. The sample aspiration and dispensing drive mechanism 1200 drives the piston shaft assembly 1120 to reciprocate, providing positive or negative pressure to the pipette tip connection 1112, thereby enabling the sample pipette tip 11120 to aspirate and dispense one or multiple times simultaneously.

[0423] In some embodiments, the piston shaft 1122 is made of a material with a low coefficient of friction and good wear resistance, such as ceramic or stainless steel. This design reduces friction during sliding of the piston shaft 1122, lowers the power loss of the sample aspiration and dispensing drive mechanism 1200, ensures drive efficiency, and prevents jamming or trajectory deviation of the piston shaft 1122 due to excessive frictional resistance, thus ensuring smooth aspiration and dispensing action and accurate dispensing. Since the piston shaft 1122 requires long-term, high-frequency reciprocating motion, the wear resistance of the material prevents rapid wear and scratches on its surface, ensuring sealing, preventing leakage, extending the service life of the piston shaft 1122, and reducing equipment maintenance and replacement costs.

[0424] See Figure 31 and Figure 32In some embodiments, the flexible fixing assembly 1123 includes a piston shaft fixing plate 11231 and a flexible member 11232. The piston shaft fixing plate 11231 is fixed below the piston shaft pressure plate 1121. The piston shaft fixing plate 11231 is provided with a first fixing groove 11231a and a second fixing groove 11231b that are connected to each other and pass through the piston shaft fixing plate 11231. The top end of the piston shaft 1122 passes through the first fixing groove 11231a and extends into the second fixing groove 11231b. The flexible member 11232 is disposed in the second fixing groove 11231b and fills the space between the piston shaft 1122 and the piston shaft fixing plate 11231, and / or fills the space between the piston shaft 1122 and the piston shaft pressure plate 1121.

[0425] Combination Figure 29 and Figure 31 In some embodiments, the piston shaft 1122 includes a shaft portion and a fixing portion 11222 disposed at the top end of the shaft portion. The rod portion 11221 is placed in a first fixing groove 11231a, and the fixing portion 11222 is placed in a second fixing groove 11231b. The top end of the fixing portion 11222 abuts against the bottom of the piston shaft pressure plate 1121, and a flexible member 11232 fills the gap between the bottom end of the fixing portion 11222 and the bottom of the groove of the second fixing groove 11231b. The flexible member 11232 fills the gap between the piston shaft pressure plate 1121 and the piston shaft fixing plate 11231, eliminating the backlash difference.

[0426] Specifically, the reciprocating sliding of the shaft portion enables the sample pipetting tip 11120 to aspirate and spray liquid; the fixing portion 11222 is configured as a protrusion 14211 with a diameter larger than that of the shaft portion, used for assembly and connection with the piston shaft fixing plate 11231 and the piston shaft pressure plate 1121. The flexible component 11232 itself has compressible deformation characteristics, which can fill the gap between the bottom end of the fixing portion 11222 and the bottom of the second fixing groove 11231b, so that the components form a tight contact.

[0427] In some embodiments, the flexible element 11232 includes a first sealing ring, a silicone pad, etc., and is made of a flexible material.

[0428] In some embodiments, the pipette tip connection portion 1112 is disposed at one end of the piston cavity assembly 1110 away from the piston shaft 1122 and communicates with the piston cavity assembly 1110. The pipette tip connection portion 1112 is used for a detachable and sealed connection with the sample pipette tip 11120. The sample pipette tip module 1000 also includes a tip retraction mechanism 1400, which includes a tip retraction plate 1410 and a tip retraction drive member 1420. The tip retraction plate 1410 is used to abut against the sample pipette tip 11120. The tip retraction drive member 1420 is drivenly connected to the tip retraction plate 1410 and is used to drive the tip retraction plate 1410 to abut against the sample pipette tip 11120 and push the sample pipette tip 11120 away from the pipette tip connection portion 1112.

[0429] See Figure 29 and Figure 30 The piston cavity assembly 1110 also includes a piston cavity 1111, which is configured as a plurality of cavity structures with independent channels or a multi-channel cavity structure.

[0430] Specifically, the piston chamber 1111 can be configured in two structures to suit different experimental scenarios based on pipetting throughput requirements. When the piston chamber 1111 is configured as a combination of several independent single-channel chambers, each corresponding to a pipetting unit, the channels are independent of each other. If one channel malfunctions or needs replacement, it will not affect the normal operation of other channels. The number of channels can be flexibly combined to meet small to medium throughput pipetting needs, while also facilitating later maintenance and component replacement. When the piston chamber 1111 is configured as a multi-channel chamber structure, its integrated chamber integrates multiple independent channels. This high integration and small space occupation enables high-throughput synchronous pipetting, significantly improving the efficiency of batch pipetting.

[0431] The pipette tip connection 1112 is disposed on the piston cavity 1111, and the pipette tip connection 1112 is provided with a suction and spray cavity for the piston shaft 1122 to be inserted. Specifically, when the piston shaft 1122 slides upward, a negative pressure is formed inside the suction and spray cavity, and liquid can be aspirated by the sample pipette tip 11120; when the piston shaft 1122 slides downward, the pressure inside the suction and spray cavity increases, and liquid is sprayed.

[0432] The bottom of the pipette tip connector 1112 is detachably and sealed to the sample pipette tip 11120. Specifically, the detachable connection allows for quick replacement of the sample pipette tip 11120, meeting the requirements for disposable consumables in experiments and avoiding cross-contamination. The sealing ensures the airtightness of the cavity during the aspiration process, preventing problems such as inaccurate pipetting volume and aspiration failure caused by air pressure leakage.

[0433] See Figure 29In some embodiments, a first receiving groove 11121 is formed on the bottom outer wall of the pipette tip connection 1112, and a second sealing ring 11122 is installed in the first receiving groove 11121. The top opening of the sample pipette tip 11120 is wrapped around the bottom outer wall of the pipette tip connection 1112, and the second sealing ring 11122 achieves a sealing connection between the pipette tip connection 1112 and the sample pipette tip 11120. When the sample pipette tip 11120 needs to be replaced, it can be disassembled by separating from the pipette tip connection 1112 through its top opening under external force. When the top opening of the sample pipette tip 11120 is wrapped around the bottom outer wall of the pipette tip connection 1112, the inner wall of the sample pipette tip 11120 will be tightly fitted with the second sealing ring 11122. The elasticity of the second sealing ring 11122 causes it to undergo a certain degree of compression deformation, filling the gap between the outer wall of the pipette tip connection 1112 and the inner wall of the sample pipette tip 11120, thus forming a reliable sealing layer between them. The first receiving groove 11121 provides installation and limiting space for the second sealing ring 11122, preventing the second sealing ring 11122 from shifting or falling off during the assembly or pipetting of the sample pipette tip 11120, ensuring the stability of the sealing structure. When replacing the sample pipette tip 11120, only a certain external force needs to be applied to overcome the friction between the second sealing ring 11122 and the inner wall of the sample pipette tip 11120, causing the top opening of the sample pipette tip 11120 to separate from the pipette tip connection 1112, completing the disassembly and replacement of the sample pipette tip 11120. Through the above settings, the requirements for the use of disposable sample pipette tips 11120 in experiments are met, cross-contamination of samples is avoided, and the reliability of the seal is maintained during pipetting.

[0434] In some embodiments, the bottom outer wall of the pipette tip connector 1112 and the inner wall of the opening of the sample pipette tip 11120 can be configured for an interference fit to form a seal. Specifically, the inner diameter of the inner wall of the opening of the sample pipette tip 11120 is slightly smaller than the outer diameter of the bottom outer wall of the pipette tip connector 1112. When the sample pipette tip 11120 is fitted onto the bottom of the pipette tip connector 1112, a certain amount of external force is required for assembly. After assembly, the inner wall of the sample pipette tip 11120 will generate a clamping force on the outer wall of the pipette tip connector 1112 due to elastic deformation, so that the two form a tight fit without gaps. With the above configuration, no additional flexible sealing elements such as sealing rings are needed to achieve a seal.

[0435] See Figure 30When the pipetting operation is completed and the sample pipetting tip 11120 needs to be replaced, the tip retraction drive 1420 outputs power to drive the tip retraction plate 1410 to move towards the sample pipetting tip 11120. After the tip retraction plate 1410 moves to the preset position, it abuts against the sample pipetting tip 11120. The tip retraction drive 1420 continues to output thrust, and the tip retraction plate 1410 transmits the force to the sample pipetting tip 11120. This force overcomes the connection force between the sample pipetting tip 11120 and the tip connection part 1112. When the force reaches the preset threshold, the sample pipetting tip 11120 will separate from the bottom outer wall of the tip connection part 1112, completing the disassembly. Afterward, the tip retraction drive 1420 drives the tip retraction plate 1410 to reset, waiting for the next tip retraction.

[0436] In some embodiments, the tip retraction drive 1420 includes a tip retraction rod 1421 and a tip retraction reset member 1422. The tip retraction rod 1421 is slidably disposed in the piston cavity 1111 and fixedly connected to the tip retraction plate 1410. The sample aspiration and dispensing drive mechanism 1200 drives the tip retraction rod 1421 to slide through the piston shaft pressure plate 1121, causing the tip retraction plate 1410 to abut against and push the sample pipette tip 11120. The tip retraction reset member 1422 is connected between the tip retraction rod 1421 and the piston cavity 1111, and / or connected between the tip retraction plate 1410 and the piston cavity 1111. The tip retraction reset member 1422 is used to drive the tip retraction rod 1421 and the tip retraction plate 1410 to reset in a direction away from the sample pipette tip 11120. This implements a sample aspiration and dispensing drive mechanism 1200 as a power source to control the aspiration and dispensing function and the tip retraction function.

[0437] Specifically, see Figure 30 , Figure 33 and Figure 34The sample aspiration and spraying mechanism 1200 first drives the piston shaft pressure plate 1121 to reciprocate and slide. Through the flexible fixing component 1123, it drives the piston shaft 1122 to move synchronously. The piston shaft 1122 changes the air pressure in the aspiration and spraying chamber of the suction head connection 1112, providing positive or negative pressure to the suction head connection 1112, thereby completing the spraying and aspiration actions. During this stage, the head retraction rod 1421 and the head retraction plate 1410 do not participate in the work and are in the standby position. When the aspiration and dispensing of liquid is completed and the sample pipette tip 11120 needs to be disassembled, the sample aspiration and dispensing drive mechanism 1200 continues to drive the piston shaft pressure plate 1121 to move towards the sample pipette tip 11120. At this time, the piston shaft pressure plate 1121 will touch the retractor rod 1421 and drive it to slide synchronously. The retractor rod 1421 then pushes the retractor plate 1410 to move towards the sample pipette tip 11120 until the retractor plate 1410 abuts against the sample pipette tip 11120 and applies a pushing force, finally overcoming the connection force between the sample pipette tip 11120 and the tip connection part 1112, and realizing the disassembly of the sample pipette tip 11120. After the tip retraction action is completed, the driving force of the sample aspiration and spraying drive mechanism 1200 is removed, and the tip retraction reset component 1422 releases its stored elastic potential energy, generating a pulling force in the direction away from the sample pipetting tip 11120, which drives the tip retraction rod 1421 and the tip retraction plate 1410 back to the initial position, preparing for the next pipetting and tip retraction cycle.

[0438] With the above settings, there is no need to configure a separate drive source for the head retraction function, which reduces the number of parts in the equipment, simplifies the overall mechanical structure, and reduces production and maintenance costs.

[0439] In some embodiments, the retraction reset member 1422 is configured as a spring. Specifically, the spring is sleeved on the outside of the retraction rod 1421, with one end of the spring abutting against the piston cavity 1111 and the other end connected to the protrusion 14211 of the retraction rod 1421, forming an axial elastic constraint. Alternatively, one end of the spring can be fixed to the bottom of the piston cavity 1111, and the other end connected to the retraction plate 1410.

[0440] Understandably, the head-down drive unit 1420 can also be configured as other power-independent drive units.

[0441] See Figure 29 In some embodiments, the sample pipetting mechanism 1100 further includes a sealing assembly 1130, which is disposed at the mating connection between the piston cavity assembly 1110 and the piston shaft assembly 1120. The sealing assembly 1130 is used to eliminate mating gaps and ensure the airtightness of the cavity during pipetting.

[0442] Combination Figure 30In some embodiments, the piston cavity assembly 1110 includes a first fixing plate 11111 and a second fixing plate 11112. The first fixing plate 11111 is installed above the piston cavity 1111, and the second fixing plate 11112 has a first through hole for sliding connection of the piston shaft 1122. The second fixing plate 11112 is installed below the piston cavity 1111, and the second fixing plate 11112 has a second through hole for the suction head connection portion 1112 to pass through.

[0443] See Figure 29 and Figure 30 In some embodiments, the sealing assembly 1130 includes a third sealing ring fitted onto the rod portion 11221 of the piston shaft 1122. Specifically, a second receiving groove 11111a is formed on the inner wall of the first through hole, and the third sealing ring is received in the second receiving groove 11111a.

[0444] It is understandable that the sealing assembly 1130 can also be configured as other sealing structures with sealing effect to achieve the sealing of the piston shaft 1122.

[0445] See Figure 26 and Figure 35 In some embodiments, the sample transfer module 10000 further includes a sample pipette tip support assembly 4000, which is used to support and position the sample pipette tip 11120. Specifically, the sample pipette tip support assembly 4000 includes a sample pipette tip base plate 4100, a sample pipette tip support plate 4200, and a sample pipette tip holder 4300. The sample pipette tip base plate 4100 is fixed on the sample pipette frame module 5000, and the sample pipette tip holder 4300 is fixed on the sample pipette tip base plate 4100 through the sample pipette tip support plate 4200. The sample pipette tip holder 4300 is used to support the sample pipette tip 11120.

[0446] The sample transfer station includes a liquid aspiration position for sample plate 0001 and a liquid spraying position for reaction plate 0002. The sample pipette tip support assembly 4000 is set in a preset working position and is located between the liquid aspiration position for sample plate 0001 and the liquid spraying position for reaction plate 0002.

[0447] In some embodiments, the quick-change pipette tip device 1300 can be configured with a bolted connection structure, etc. Other detachable connection structures can be configured according to actual needs, such as snap-fit ​​connections, magnetic connections, pin connections, etc. Different connection structures can adapt to different usage scenarios. For example, snap-fit ​​connections can achieve tool-free quick assembly and disassembly, while magnetic connections can reduce mechanical wear during assembly and disassembly. The overall configuration can be flexibly selected based on factors such as the weight of the sample pipetting mechanism 1100, usage frequency, and accuracy requirements.

[0448] See Figure 36In some embodiments, the quick-change device 1300 includes a positioning module 1310 and a quick-change module 1320. The positioning module 1310 is used to position the sample pipetting mechanism 1100. The quick-change module 1320 includes a clamping mechanism 1321 and a quick-change drive mechanism 1322. The clamping mechanism 1321 has a clamping state and a loosening state that clamps or loosens the sample pipetting mechanism 1100 positioned at the positioning module 1310. The quick-change drive mechanism 1322 is driven to the clamping mechanism 1321 and drives the clamping mechanism 1321 to switch between the clamping state and the loosening state.

[0449] Through the above settings, the positioning module 1310 achieves accurate and stable pre-positioning of the sample pipetting mechanism 1100, preventing the sample pipetting mechanism 1100 from shifting or shaking during disassembly, assembly, or operation; the quick-change drive mechanism 1322 of the quick-change module 1320 can drive the clamping mechanism 1321 to flexibly switch between the clamping state and the loosening state. In the clamping state, it can reliably lock the positioned sample pipetting mechanism 1100, ensuring the structural stability during pipetting; in the loosening state, it can quickly release the constraint, and the sample pipetting mechanism 1100 can be disassembled without complicated operations. The pre-positioning function of the positioning module 1310 eliminates the need for repeated calibration during the installation of the traditional sample pipetting mechanism 1100. Operators only need to place the sample pipetting mechanism 1100 into the positioning module 1310 for initial fixation. The quick-change module 1320 directly controls the state switching of the clamping mechanism 1321 via the quick-change drive mechanism 1322. Locking or loosening the sample pipetting mechanism 1100 can be achieved simply by operating the quick-change drive mechanism 1322, significantly simplifying the assembly and disassembly process and reducing the time required for a single sample pipetting mechanism 1100 replacement to a short time, thus solving the problem of cumbersome assembly and disassembly using traditional screw connections. In high-throughput experimental scenarios, it is often necessary to frequently change sample pipetting mechanisms 1100 of different specifications or those that have already been aspirated or displaced. The modular structure of this device enables rapid assembly and disassembly of the sample pipetting mechanism 1100, and the coordinated actions of positioning and quick-change have high repeatability, meeting the operational requirements for high-frequency replacement of the sample pipetting mechanism 1100 and avoiding interruptions to the experimental process due to excessively long replacement procedures. In summary, by working together with the positioning module 1310 and the quick-change module 1320, the sample pipetting mechanism 1100 can be quickly locked and disassembled, making the sample pipetting mechanism 1100 easy to install and disassemble with short time consumption, meeting the needs of rapid head changing in high-throughput experimental scenarios and improving experimental efficiency.

[0450] See Figure 36 and Figure 37In some embodiments, the positioning module 1310 includes a positioning groove 1311, which is provided with a positioning groove 13111, a positioning inlet 13112, and a clamping port 13113 communicating with the positioning groove 13111. The sample pipetting mechanism 1100 extends into the positioning groove 13111 through the positioning inlet 13112. In the clamping state, the clamping mechanism 1321 extends into the positioning groove 13111 through the clamping port 13113 to apply a clamping force to the sample pipetting mechanism 1100. The positioning groove 13111, which is used to accommodate the sample pipetting mechanism 1100, is adapted in shape and size to the part of the sample pipetting mechanism 1100 that needs to be positioned. The positioning inlet 13112 provides a clear guide path for the insertion of the sample pipetting mechanism 1100, which facilitates quick alignment by the operator.

[0451] With the above setup, the operator pushes the sample pipetting mechanism 1100 into the positioning groove 13111 through the positioning inlet 13112. The positioning groove 13111 limits the sample pipetting mechanism 1100, keeping it in a preset installation posture and completing the initial position fixation to prevent the sample pipetting mechanism 1100 from shifting. When it is necessary to completely fix the sample pipetting mechanism 1100, the quick-change drive mechanism 1322 of the quick-change module 1320 will drive the clamping mechanism 1321 to move. The clamping mechanism 1321 extends into the positioning groove 13111 through the clamping port 13113, directly acting on the designated part of the sample pipetting mechanism 1100 and applying clamping force to firmly lock the sample pipetting mechanism 1100 in the positioning groove 13111, ensuring that the sample pipetting mechanism 1100 will not loosen during operation.

[0452] In some embodiments, the positioning module 1310 further includes a positioning baffle 1312, which is disposed at one end of the positioning groove 1311 away from the positioning inlet 13112, for the sample pipetting mechanism 1100 to abut against. The positioning baffle 1312 is a limiting component installed at the end of the positioning groove 1311, away from the positioning inlet 13112, providing a precise stop for the axial travel of the sample pipetting mechanism 1100, defining the final installation position of the sample pipetting mechanism 1100 in the positioning groove 13111, and preventing the sample pipetting mechanism 1100 from deviating in position due to being pushed too deep or too shallow.

[0453] With the above setup, when the operator pushes the sample pipetting mechanism 1100 into the positioning groove 13111 through the positioning inlet 13112, the sample pipetting mechanism 1100 will move along the guide direction of the positioning groove 13111 until its end abuts against the positioning baffle 1312. At this point, the sample pipetting mechanism 1100 reaches the preset standard installation position. No additional calibration is required; the operator can directly determine whether the sample pipetting mechanism 1100 is properly positioned through the abutment action. The sample pipetting mechanism 1100 is ultimately constrained to the same position by the positioning baffle 1312, ensuring that sample pipetting mechanisms 1100 installed by different batches and by different personnel are in the same working posture, avoiding the impact of installation position differences on subsequent pipetting accuracy. This eliminates the need for manual measurement or observation of whether it is in place; precise positioning can be achieved solely through mechanical abutment, lowering the operational threshold and improving the ease of assembly and disassembly.

[0454] In some embodiments, the clamping mechanism 1321 includes a clamping block 13211, which is slidably connected to the clamping port 13113. The clamping block 13211 is provided with a clamping surface 13211a, which is used to extend into the positioning groove 13111 and abut against the sample pipetting mechanism 1100. The quick-change drive mechanism 1322 drives the clamping block 13211 to slide back and forth in the clamping port 13113, so as to drive the clamping surface 13211a to abut or separate from the sample pipetting mechanism 1100. Specifically, the sliding connection between the clamping block 13211 and the clamping port 13113 ensures that the clamping block 13211 can smoothly perform linear reciprocating motion within the clamping port 13113 without jamming. The clamping surface 13211a matches the contour of the part to be clamped in the sample pipetting mechanism 1100, which increases the contact area with the sample pipetting mechanism 1100, allowing the clamping force to be applied evenly and avoiding excessive local stress that could damage the sample pipetting mechanism 1100. At the same time, the clamping port 13113 provides precise motion guidance and stroke constraint for the clamping block 13211, limiting the range of motion of the clamping block 13211.

[0455] With the above settings, in the clamping state, the quick-change drive mechanism 1322 outputs power to push the clamping block 13211 to slide along the clamping port 13113 toward the positioning groove 13111. At this time, the clamping surface 13211a of the clamping block 13211 will extend into the positioning groove 13111 and abut against the surface of the sample pipetting mechanism 1100 that has been positioned, and continuously apply pressure to firmly lock the sample pipetting mechanism 1100 in the positioning groove 13111, ensuring that the sample pipetting mechanism 1100 will not loosen or shift when it is working. When the device is released, the quick-change drive mechanism 1322 reverses its direction, causing the clamping block 13211 to slide along the clamping port 13113 away from the positioning groove 13111. The clamping surface 13211a then separates from the sample pipetting mechanism 1100, releasing the pressure constraint on the sample pipetting mechanism 1100. At this point, the operator can easily remove the sample pipetting mechanism 1100 from the positioning groove 13111, completing the disassembly.

[0456] See Figure 38 In some embodiments, the quick-change drive mechanism 1322 includes a movable cam block 13221 and a linear drive member 13222. The movable cam block 13221 is provided with a cam inclined surface 13221a for abutting against the clamping block 13211. The linear drive member 13222 is drivenly connected to the movable cam block 13221 and can drive the movable cam block 13221 to reciprocate linearly, thereby driving the clamping block 13211 to slide within the clamping port 13113. The quick-change drive mechanism 1322 realizes the conversion of power direction through the transmission of the cam inclined surface 13221a. The overall motion process is divided into two states: clamping drive and releasing drive. Specifically:

[0457] In the clamping state, the linear drive 13222 outputs a positive driving force, pushing the moving cam block 13221 to move linearly. At this time, the cam inclined surface 13221a on the moving cam block 13221 will abut against the contact surface of the clamping block 13211. As the moving cam block 13221 continues to move forward, the cam inclined surface 13221a will generate an upward component force on the clamping block 13211, pushing the clamping block 13211 to slide upward along the clamping port 13113 until the clamping surface 13211a of the clamping block 13211 abuts against the sample pipetting mechanism 1100 and completes the locking.

[0458] In the released state, the linear drive 13222 outputs a reverse driving force, pulling the moving cam block 13221 to perform a linear reset motion. The contact pressure between the cam inclined surface 13221a and the clamping block 13211 gradually disappears. After the clamping block 13211 loses the support force of the inclined surface, it will slide down along the clamping port 13113, releasing the clamping constraint on the sample pipetting mechanism 1100 and completing the release action.

[0459] Through the above configuration, the cam inclined surface 13221a converts the horizontal linear motion of the linear drive component 13222 into the vertical lifting motion of the clamping block 13211. This eliminates the need for additional reversing transmission components, resulting in a shorter power transmission path, lower losses, and improved drive efficiency. The tilt angle of the cam inclined surface 13221a is positively correlated with the linear stroke of the moving cam block 13221 and the lifting stroke of the clamping block 13211. By controlling the moving distance of the moving cam block 13221, the clamping stroke and clamping force of the clamping block 13211 can be precisely adjusted. If the quick-change drive mechanism 1322 has multiple clamping blocks 13211, multiple cam inclined surfaces 13221a can be positioned at corresponding locations on one moving cam block 13221 to simultaneously drive all clamping blocks 13211 to lift and lower synchronously, ensuring consistent locking force at each clamping point and improving the stability of the sample pipetting mechanism 1100.

[0460] In some embodiments, the linear drive 13222 includes a drive plate 132221 and a locking shaft 132222. The drive plate 132221 is mounted on the positioning groove 1311, and the locking shaft 132222 is threadedly connected to the drive plate 132221. The locking shaft 132222 is provided with a drive end 132222a, which is rotatably connected to the movable cam block 13221. The drive plate 132221 provides stable support and motion guidance for the locking shaft 132222, and the drive plate 132221 is machined with an internal threaded hole adapted to the locking shaft 132222 for threaded connection.

[0461] With the above configuration, when the operator rotates the locking shaft 132222, since the locking shaft 132222 and the drive plate 132221 are threadedly connected, the rotational motion of the locking shaft 132222 is converted into a linear reciprocating motion along its own axis. This, in turn, pushes or pulls the movable cam block 13221, which is rotatably connected to it, causing it to reciprocate along a preset path. The movable cam block 13221 then transmits the linear power to the clamping block 13211 through its own cam inclined surface 13221a, completing the clamping or loosening action on the sample pipetting mechanism 1100. The threaded drive has a fixed lead; for every fixed angle rotated, the locking shaft 132222 produces a fixed distance of linear displacement. By controlling the number of rotations of the locking shaft 132222, the stroke of the movable cam block 13221 can be precisely adjusted, thereby controlling the clamping force and stroke of the clamping block 13211, ensuring consistent locking performance. The drive can be completed by manually rotating the locking shaft 132222, without the need for special tools, which improves the convenience of the overall operation.

[0462] In some embodiments, one end of the locking shaft 132222 is provided with an operating part 132222b exposed to the drive plate 132221, which is provided with a knob or anti-slip texture to facilitate operation.

[0463] See Figure 39 In some embodiments, the quick-change module 1320 further includes a quick-change reset mechanism 1330, which is drivenly connected to the clamping block 13211 and applies a driving force to the clamping block 13211 toward restoring it to the released state. The elastic force of the quick-change reset mechanism 1330 does not directly dominate the releasing action of the clamping block 13211, but plays a role in assisting the releasing and resetting with less effort. Specifically, when the quick-change drive mechanism 1322 applies a locking driving force, the elastic reset force generated by the quick-change reset mechanism 1330 is completely canceled out by the driving force of the quick-change drive mechanism 1322, and the threaded drive of the quick-change drive mechanism 1322 has a self-locking characteristic, which can firmly lock the clamping position of the clamping block 13211, preventing the quick-change reset mechanism 1330 from popping the clamping block 13211 open, thus ensuring the stability of the locked state of the sample pipetting mechanism 1100. When the operator reverses the quick-change drive mechanism 1322 to release the locking drive force, the quick-change reset mechanism 1330 will immediately release the stored elastic force, providing auxiliary power for the reset of the clamping block 13211. The operator only needs to apply a small reverse drive force to drive the clamping block 13211 to quickly return to the loosened state, which greatly reduces the force required for the loosening operation, simplifies the disassembly process of the sample pipetting mechanism 1100, and strengthens the core function of quick-change of the device.

[0464] In the clamped state, the locking force of the quick-change drive mechanism 1322 is greater than the elastic force of the positioning mechanism, resulting in a force balance. The elastic force of the quick-change reset mechanism 1330 exists only as a reserve force and cannot exceed the locking force of the quick-change drive mechanism 1322, thus not affecting the locking stability. In the released state, the locking force of the quick-change drive mechanism 1322 gradually decreases, and the elastic force of the quick-change reset mechanism 1330 changes from being canceled out to becoming an auxiliary force, forming a combined force with the operator's reverse operating force to achieve effortless reset. The elastic parameters of the quick-change reset mechanism 1330 are precisely calculated to ensure that its elastic force meets the effort-saving requirements for assisted release without exceeding the self-locking critical force of the quick-change drive mechanism 1322.

[0465] With the above settings, in the driving state, the cam inclined surface 13221a of the moving cam block 13221 drives the clamping block 13211 to slide along the clamping port 13113 toward the positioning groove 13111. The quick-change reset mechanism 1330 generates elastic deformation and accumulates potential energy as the clamping block 13211 moves. The reset force increases positively correlated with the clamping stroke. Since the locking driving force output by the quick-change drive mechanism 1322 is always greater than the reset force, the two form a force balance, and the clamping block 13211 can stably stay in the clamping position. In the unlocked state, the quick-change drive mechanism 1322 pulls the moving cam block 13221 backward, and the driving force it applies to the clamping block 13211 is gradually dissipated. The elastic potential energy of the quick-change reset mechanism 1330 is then released. The reset force and the operator's reverse operating force form a resultant force, which drives the clamping block 13211 to slide in the opposite direction along the clamping port 13113 to the initial release position, thereby releasing the clamping constraint on the sample pipetting mechanism 1100.

[0466] Specifically, in some embodiments, the quick-change reset mechanism 1330 includes a spring, a torsion spring, an elastic structure, etc.

[0467] In some embodiments, the quick-change reset mechanism 1330 includes a first quick-change elastic reset member 1331, which is connected between the clamping block 13211 and the positioning groove 1311, and applies a first driving force to the clamping block 13211 to separate it from the sample pipetting mechanism 1100.

[0468] Specifically, the first quick-change elastic reset component 1331 is configured as a first spring, and the positioning groove 1311 has a first spring seat 13114. One end of the first spring is fixedly disposed in the first spring seat 13114, and the other end is connected to the clamping block 13211. When the quick-change drive mechanism 1322 pushes the clamping block 13211 to slide along the clamping port 13113 toward the positioning groove 13111 by the inclined surface of the moving cam block 13221, the first spring will be compressed with the displacement of the clamping block 13211, and the elastic potential energy will be accumulated simultaneously. At this time, the first driving force generated by the first spring will increase with the increase of the clamping stroke, but this force will be completely offset by the locking driving force of the quick-change drive mechanism 1322. Moreover, the threaded self-locking structure of the quick-change drive mechanism 1322 can stably lock the position of the clamping block 13211, so it will not affect the locking stability of the sample pipetting mechanism 1100. When the operator reverses the locking shaft 132222, the locking driving force of the quick-change drive mechanism 1322 gradually disappears. The first spring will quickly release the accumulated elastic potential energy and apply a first driving force to the clamping block 13211 in the direction away from the sample pipetting mechanism 1100. This force, together with the operator's reverse operating force, will cause the clamping block 13211 to reset along the clamping port 13113 until the clamping block 13211 is completely separated from the sample pipetting mechanism 1100, thus releasing the clamping constraint.

[0469] In some embodiments, the quick-change reset mechanism 1330 includes a second quick-change elastic reset member 1332, which is drivenly connected to the movable cam block 13221. In the pressed state, the movable cam block 13221 slides to the pressed position, and in the released state, the movable cam block 13221 slides to the released position. The second quick-change elastic reset member 1332 applies a second driving force to the movable cam block 13221 to slide from the pressed position to the released position.

[0470] Specifically, the second quick-change elastic reset member 1332 is configured as a second spring, and a second spring seat 13121 is provided on the positioning baffle 1312. One end of the first spring is fixedly installed in the second spring seat 13121 of the positioning baffle 1312, and the other end is connected to the moving cam block 13221.

[0471] With the above settings, when the quick-change drive mechanism 1322 drives the moving cam block 13221 to slide towards the pressing position, the second spring will be compressed along with the displacement of the moving cam block 13221, and the elastic potential energy will be accumulated simultaneously. At this time, the second driving force generated by the spring will increase with the increase of the cam block stroke, but this force will be completely offset by the locking driving force of the quick-change drive mechanism 1322. At the same time, the thread self-locking characteristic of the quick-change drive mechanism 1322 can firmly lock the position of the moving cam block 13221. Therefore, the moving cam block 13221 can be stably kept in the pressing position, ensuring the locking effect on the sample pipetting mechanism 1100. When the operator reverses the locking shaft 132222, the locking driving force of the quick-change drive mechanism 1322 gradually disappears. The second spring will quickly release the accumulated elastic potential energy and apply a second driving force toward the loosened position to the moving cam block 13221. This force, together with the operator's reverse operating force, will drive the moving cam block 13221 to reset to the loosened position. Then, through the transmission of the cam inclined surface 13221a, it will assist the clamping block 13211 to return to its original position, releasing the clamping constraint on the sample pipetting mechanism 1100.

[0472] In the embodiments of this application, compared with the structure that only sets the first quick-change elastic reset member 1331, the second quick-change elastic reset member 1332 provides an auxiliary second driving force from the end of the moving cam block 13221, which forms a double assistance with the first driving force at the end of the clamping block 13211, further reducing the force required for the operator to release the operation, greatly shortening the disassembly time of the sample pipetting mechanism 1100, and enhancing the quick-change function of the device.

[0473] See Figure 39In some embodiments, the quick-change drive mechanism 1322 further includes a base plate 13223, which is disposed at the bottom end of the positioning groove 1311 and abuts against the bottom surface of the moving cam block 13221; the bottom surface of the positioning groove 1311 abuts against the top surface of the moving cam block 13221; wherein, the base plate 13223 and the positioning groove 1311 enclose and construct a drive slide 132231, and the moving cam block 13221 switches between the pressing position and the releasing position in the drive slide 132231 through reciprocating linear motion. Specifically, the base plate 13223 can be configured as a rigid flat plate structure, which is fixed to the bottom of the positioning groove 1311 at a preset installation position by bolts or slots. Its top surface is completely in contact with the bottom surface of the moving cam block 13221. The inner bottom surface of the positioning groove 1311 facing the moving cam block 13221, that is, the lower surface of the groove, is in close contact with the top surface of the moving cam block 13221. The two form a bidirectional limit on the moving cam block 13221, which restricts the displacement of the moving cam block 13221 in the vertical direction and prevents the cam block from moving up and down during the movement.

[0474] With the above settings, when the operator rotates the locking shaft 132222 and the quick-change drive mechanism 1322 outputs a positive driving force, the moving cam block 13221 will slide linearly along the drive slide groove 132231 towards the positioning groove 13111 under the action of the driving force until it reaches the preset pressing position. During this process, the directional constraint of the slide groove can ensure that the movement trajectory of the cam block does not deviate, and the cam inclined surface 13221a on its surface can accurately fit with the driven inclined surface of the pressing block 13211, realizing stable power transmission. When the quick-change drive mechanism 1322 outputs a reverse driving force and the second quick-change elastic reset member 1332 provides auxiliary reset power, the moving cam block 13221 will perform a linear reset movement along the drive slide groove 132231 away from the positioning groove 13111 until it returns to the preset release position.

[0475] See Figure 36 and Figure 40 In some embodiments, the positioning module 1310 includes a cavity positioning submodule 1301, which is used to position the piston cavity assembly 1110 of the sample pipetting mechanism 1100.

[0476] In some embodiments, the positioning module 1310 includes a shaft positioning submodule 1302, which is used to position the piston shaft assembly 1120 of the sample pipetting mechanism 1100.

[0477] In some embodiments, the sample pipetting mechanism 1100 includes a sample aspiration and dispensing driving mechanism 1200 and a sample pipetting mechanism 1100. The sample pipetting mechanism 1100 includes a piston cavity assembly 1110 and a piston shaft assembly 1120. The piston cavity assembly 1110 includes a sample pipetting tip 11120. The piston shaft assembly 1120 is slidably connected to the piston cavity assembly 1110. The sample aspiration and dispensing driving mechanism 1200 is driven to slide back and forth on the piston shaft assembly 1120 to aspirate and dispense liquid from the sample pipetting tip 11120.

[0478] In some embodiments, the piston shaft assembly 1120 includes a piston shaft pressure plate 1121 and a piston shaft 1122. The piston shaft pressure plate 1121 is used to connect to the pipette tip quick-change device 1300 and is detachably connected to the sample aspiration and dispensing drive mechanism 1200 via the pipette tip quick-change device 1300. The piston shaft 1122 is slidably connected to the piston cavity assembly 1110.

[0479] In some embodiments, the piston cavity assembly 1110 further includes a piston cavity 1111 and a pipette tip connection portion 1112. The piston cavity 1111 is configured as a plurality of cavity structures with independent channels or a multi-channel cavity structure. The pipette tip connection portion 1112 is disposed on the piston cavity 1111 and is provided with a suction and spraying cavity for the piston shaft 1122 to be inserted. The bottom of the pipette tip connection portion 1112 is detachably and sealingly connected to the sample pipette tip 11120.

[0480] Specifically, in some embodiments, the piston cavity 1111 of the sample pipetting mechanism 1100 is provided with first quick-change parts 1111a on both sides. When the cavity positioning submodule 1301 is used to position the piston cavity assembly 1110 of the sample pipetting mechanism 1100, the positioning groove 1311 of the cavity positioning submodule 1301 is used to cooperate and connect with the first quick-change parts 1111a.

[0481] Specifically, in some embodiments, the piston shaft pressure plate 1121 of the sample pipetting mechanism 1100 is provided with second quick-change parts 1121a on both sides. When the shaft positioning submodule 1302 is used to position the piston shaft assembly 1120 of the sample pipetting mechanism 1100, the positioning groove 1311 of the shaft positioning submodule 1302 is used to cooperate and connect with the second quick-change parts 1121a.

[0482] In some embodiments, each positioning groove 13111 in the cavity positioning submodule 1301 is provided with two clamping blocks 13211, and each positioning groove 13111 in the shaft positioning submodule 1302 is provided with one clamping block 13211. The piston cavity assembly 1110 is larger and heavier than the piston shaft assembly 1120. Single-point clamping can easily lead to uneven force on the piston cavity assembly 1110, and may even cause localized stress concentration that damages the piston cavity assembly 1110. Two symmetrically distributed clamping blocks 13211 can simultaneously apply clamping force from both sides of the first quick-change part 1111a, so that the locking force is evenly distributed on both sides of the cavity. This ensures both the firmness of the locking and maintains the horizontal orientation of the cavity, preventing the sample pipetting tip 11120 from tilting due to unilateral force, thus affecting pipetting accuracy. The piston shaft assembly 1120 also needs to reciprocate and move up and down with the first drive component. A single clamping block 13211 can eliminate the axial clearance of the piston shaft 1122. The piston shaft assembly 1120 is lightweight and small in size, and the locking force of single-point clamping can meet the fixing requirements.

[0483] In this embodiment of the application, compared to fixing the piston cavity assembly 1110 only through the quick-change pipette head device 1300, adding the fixing of the piston shaft assembly 1120 can eliminate the gap of the piston shaft 1122 and improve the pipetting accuracy.

[0484] In use, the quick-change pipette tip device 1300 in this embodiment is used by inserting the sample pipetting mechanism 1100 into the quick-change pipette tip device 1300, inserting the piston cavity 1111 of the sample pipetting mechanism 1100 into the positioning groove 1311 of the cavity positioning submodule 1301, inserting the piston shaft pressure plate 1121 into the positioning groove 1311 of the shaft positioning submodule 1302, and pushing the sample pipetting mechanism 1100 to the position where the piston cavity 1111 abuts against the positioning baffle 1312. The locking shaft 132222 is turned by hand, and the locking shaft 132222 rotates and moves inward at the same time, pushing the moving cam block 13221 to move inward, pushing the clamping block 13211 to move upward, locking the piston cavity 1111 and the piston shaft pressure plate 1121, and fixing the sample pipetting mechanism 1100. Tighten the locking shaft 132222 by hand, and the locking shaft 132222 moves outward, pushing the moving cam block 13221 to move outward. The clamping block 13211 moves downward under the action of the quick-change reset mechanism 1330, releasing the piston chamber 1111 and the piston shaft pressure plate 1121, and the sample pipetting mechanism 1100 is released.

[0485] In some embodiments, the sample pipetting head module 1000 includes a pair of interface sample pipetting side plates 1600, and a sample pipetting mechanism 1100 and a quick-change pipetting device are installed between the two interface sample pipetting side plates 1600. Specifically, an interface connection plate 1610 is provided on the top of the interface sample pipetting side plate 1600 for connecting to the sample pipetting arm 3000.

[0486] A quick-change pipette tip device 1300 is connected to the bottom of the two-interface sample pipetting side plate 1600, which is fixedly connected to the cavity positioning submodule 1301. The positioning groove 1311 of the cavity positioning submodule 1301 is used to connect with the first quick-change parts 1111a on both sides of the piston cavity 1111 of the sample pipetting mechanism 1100. The piston cavity assembly 1110 is detachably connected to the interface sample pipetting side plate 1600 through the quick-change pipette tip device 1300.

[0487] An interface mounting plate 1620 is fixedly connected between the two sample pipetting side plates 1600. A sample aspiration and dispensing drive mechanism 1200, configured as a lead screw motor, is mounted on the interface mounting plate 1620. The output shaft of the lead screw motor is fixedly connected to the positioning groove 1311 of a pipetting head quick-change device 1300, i.e., it is fixedly connected to the shaft positioning submodule 1302. The positioning groove 1311 of the shaft positioning submodule 1302 is used to engage with the second quick-change parts 1121a on both sides of the piston shaft pressure plate 1121 of the sample pipetting mechanism 1100. The piston shaft assembly 1120 is detachably connected to the sample aspiration and dispensing drive mechanism 1200 via the pipetting head quick-change device 1300.

[0488] With the above configuration, the sample pipetting head module 1000 uses the cavity positioning submodule 1301 and the shaft positioning submodule 1302 to respectively position and lock the piston cavity assembly 1110 and the piston shaft assembly 1120. Compared with the traditional structure that only fixes the cavity, this design eliminates the axial and radial clearances of the piston shaft 1122, ensuring the precise controllability of the piston shaft 1122's movement stroke and improving the quantitative and positioning accuracy of pipetting.

[0489] In some embodiments, to improve the stability of the shaft positioning submodule 1302 and the piston shaft assembly 1120 during movement, while ensuring the accuracy of liquid aspiration and dispensing by the sample pipette tip 11120, the sample pipette tip module 1000 is further provided with a first guide mechanism 1700. The first guide mechanism 1700 includes a first guide bearing 1710 mounted on the interface mounting plate 1620, and a first guide shaft 1720 connected inside the first guide bearing 1710. The bottom end of the first guide shaft 1720 extends downward and is fixedly connected to the positioning groove 1311 of the shaft positioning submodule 1302. The first guide bearing 1710 and the first guide shaft 1720 provide motion guidance for the shaft positioning submodule 1302 and the piston shaft assembly 1120, further improving the stability of the piston shaft assembly 1120 during movement and ensuring smooth and accurate liquid aspiration and dispensing.

[0490] See Figure 41 and Figure 42In some embodiments, the sample pipetting device cleaning equipment 2000 includes a tank mechanism 2010, a channel mechanism 2020 disposed within the tank mechanism 2010, a water inlet mechanism 2030 installed on the tank mechanism 2010, and a first drainage mechanism 2040 installed on the tank mechanism 2010.

[0491] See Figure 41 and Figure 42 At least one embodiment of this application provides a sample pipetting device cleaning device 2000, which includes a tank mechanism 2010, a channel mechanism 2020, a water inlet mechanism 2030, and a first drainage mechanism 2040. The channel mechanism 2020 is disposed within the tank mechanism 2010 and includes a plurality of independent sample transfer cleaning channels 2021, each sample transfer cleaning channel 2021 allowing at least one sample to be cleaned to extend into it; the water inlet mechanism 2030 is mounted on the tank mechanism 2010 and connected to the sample transfer cleaning channels 2021 for inputting cleaning fluid into the sample transfer cleaning channels 2021; the first drainage mechanism 2040 is mounted on the tank mechanism 2010 and connected to the sample transfer cleaning channels 2021 for discharging the cleaning fluid.

[0492] According to the sample pipetting device cleaning equipment 2000 of this application embodiment, during the cleaning process, the water inlet mechanism 2030 delivers cleaning solution to the sample transfer cleaning channel 2021. The cleaning solution cleans the sample to be cleaned within the sample transfer cleaning channel 2021, and the cleaning solution within the sample transfer cleaning channel 2021 is discharged through the first drainage mechanism 2040. When the sample to be cleaned is configured as a sample pipetting tip 11120 of the pipetting device, the pipetting device drives the sample pipetting tip 11120 to move, so that the sample pipetting tip 11120 is inserted one by one into the corresponding independent sample transfer cleaning channel 2021. By simulating the repeated aspiration and dispensing of cleaning solution through the sample pipetting tip 11120, the cleaning solution fully contacts the inner and outer walls of the sample pipetting tip 11120, removing residual liquid, impurities, or contaminants on the sample pipetting tip 11120, thereby achieving the cleaning effect. Since the sample transfer and cleaning channels 2021 corresponding to each sample pipette tip 11120 are independent, the problem of cross-contamination between sample pipette tips 11120 can be avoided, ensuring the cleanliness of the cleaning and improving the accuracy of experimental or test results.

[0493] In some embodiments, the cleaning solution is prepared as pure water.

[0494] In some embodiments, the cleaning fluid may also be configured as other cleaning fluids corresponding to cleaning needs.

[0495] In some embodiments, when the number of sample pipette tips 11120 in the pipetting device is configured to be 384, the number of sample transfer cleaning channels 2021 is correspondingly provided to be 384. The sample transfer cleaning channels 2021 are arranged vertically, and their inner diameter is adapted to the outer diameter of the sample pipette tips 11120, which facilitates the insertion of the sample pipette tips 11120 and improves the utilization efficiency of the cleaning solution.

[0496] Understandably, the number and size of the sample transfer and cleaning channels 2021 can be flexibly adjusted according to the specifications of the items to be cleaned. For example, for sample pipette tips 11120 with 8 channels, 24 channels, 96 channels, 384 channels, or even 1536 channels, the same number of sample transfer and cleaning channels 2021 can be set up accordingly. There is no need to change the main structure of the equipment; only the channel mechanism 2020 needs to be replaced to meet the cleaning requirements of different throughputs, thereby reducing the user's equipment procurement costs.

[0497] See Figure 42 In some embodiments, the tank mechanism 2010 includes a water inlet chamber 2011 and a drain chamber 2012. The water inlet chamber 2011 is connected to the water inlet mechanism 2030. The drain chamber 2012 is independent of the water inlet chamber 2011 and is connected to the first drain mechanism 2040. The sample transfer and cleaning channel 2021 connects the water inlet chamber 2011 and the drain chamber 2012. The cleaning fluid passes sequentially through the water inlet mechanism 2030, the water inlet chamber 2011, the sample transfer and cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040.

[0498] Specifically, the drain chamber 2012 and the inlet chamber 2011 are independent of each other, ensuring that the clean cleaning solution in the inlet chamber 2011 does not mix with the contaminated cleaning solution in the drain chamber 2012, thus preventing contamination of the cleaning solution in the inlet chamber 2011. The inlet mechanism 2030, connected to the inlet chamber 2011, delivers clean cleaning solution to the inlet chamber 2011; the first drain mechanism 2040, connected to the drain chamber 2012, discharges the contaminated cleaning solution from the drain chamber 2012. Together, they form a complete closed-loop path for the cleaning solution through the inlet mechanism 2030, the inlet chamber 2011, the sample transfer cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040, ensuring a fixed flow direction and no backflow.

[0499] See Figure 43 In some embodiments, the water inlet chamber 2011 includes a plurality of water inlet single chambers 20111, each water inlet single chamber 20111 being connected to the water inlet mechanism 2030; the water inlet mechanism 2030 includes a plurality of water inlet connectors 2031; each water inlet single chamber 20111 is connected to at least two water inlet connectors 2031.

[0500] The single-chamber inlet 20111 enables zoned liquid supply. Compared to the high-flow-rate requirement of a single liquid supply, the zoned liquid supply in this embodiment has a certain buffering and pressure-stabilizing effect. Specifically, the clean cleaning fluid delivered by the inlet mechanism 2030 first enters the single-chamber inlet 20111 of the inlet chamber 2011. The single-chamber inlet 20111 buffers and stabilizes the cleaning fluid, avoiding uneven delivery of the cleaning fluid caused by fluctuations in inlet pressure. The single-chamber inlet 20111 delivers the cleaning fluid more evenly to the sample transfer and cleaning channel 2021 corresponding to each single-chamber inlet 20111, meeting the requirements of high-throughput experiments for uniformity of cleaning effect.

[0501] In addition, if one inlet connector 2031 of a certain inlet single chamber 20111 fails due to pipeline blockage, the other inlet connector 2031 can still maintain most of the liquid supply to the single chamber, avoiding the shutdown of the sample transfer and cleaning channel 2021 corresponding to the entire inlet single chamber 20111 due to the failure of a single connector, thus improving the fault tolerance and reliability of the equipment operation.

[0502] See Figure 43 In some embodiments, four inlet chambers 20111 are provided, each inlet chamber 20111 connected to two inlet connectors 2031. Specifically, a cross-shaped partition 20112 is provided inside the inlet chamber 2011; the cross-shaped partition 20112 divides the inlet chamber 20111 into four independent inlet chambers 20111 with uniform volume. It can be understood that when the sample pipetting tips 11120 of the pipetting device are configured with 384, each inlet chamber 20111 is connected to 96 sample transfer and cleaning channels 2021, ensuring that the cleaning solution supply to all sample transfer and cleaning channels 2021 is as consistent as possible.

[0503] With the above settings, the water intake of the water inlet chamber 2011 is distributed in each independent water inlet chamber 20111, thereby improving the uniformity and stability of the water intake.

[0504] Understandably, depending on specific cleaning needs, the water inlet chamber 2011 can be divided into different numbers and / or different volumetric single water inlet chambers 20111 using partitions of different shapes and positions. Each single water inlet chamber 20111 can be connected to a different number of water inlet connectors 2031, such as two, four, or six, and the number and position of the water inlet connectors 2031 are variable.

[0505] In some embodiments, the drain chamber 2012 is located above the inlet chamber 2011; the cleaning fluid passes sequentially from bottom to top through the inlet mechanism 2030, the inlet chamber 2011, the sample transfer cleaning channel 2021, the drain chamber 2012, and the first drain mechanism 2040. It is not necessary to consider the flow rate of the cleaning fluid in the sample transfer cleaning channel 2021; only the drainage flow rate and drainage time of the drain mechanism need to be considered.

[0506] Specifically, when the drain chamber 2012 is located above the inlet chamber 2011, the cleaning fluid needs to overcome gravity and flow upwards under the combined action of the pressure from the inlet mechanism 2030 and the suction pull from the first drain mechanism 2040. The inlet mechanism 2030 delivers clean cleaning fluid to the lower inlet chamber 2011. After a stable pressure is formed in the inlet chamber 2011, the cleaning fluid flows upwards into the upper drain chamber 2012 through the sample transfer cleaning channel 2021 and is finally discharged by the first drain mechanism 2040. During this process, the cleaning fluid always maintains a unidirectional flow state in the sample transfer cleaning channel 2021. The contaminated cleaning fluid after use will quickly enter the drain chamber 2012 due to the suction of the first drain mechanism 2040, and will not stagnate in the sample transfer cleaning channel 2021 or flow back to the inlet chamber 2011 due to gravity, thus completely avoiding the problem of contaminated cleaning fluid mixing with clean cleaning fluid.

[0507] In addition, the upward flow of the cleaning fluid is guided by the combined action of the inlet water pressure and the drainage suction, and the fluid flow state in the sample transfer cleaning channel 2021 is controlled by the drainage end of the first drainage mechanism 2040. As long as the drainage flow rate of the first drainage mechanism 2040 is large enough and the drainage time is long enough, it can be ensured that there is always fresh cleaning fluid replenishment in the sample transfer cleaning channel 2021 without the need to frequently adjust the inlet water flow rate.

[0508] See Figure 43 and Figure 44 In some embodiments, the tank mechanism 2010 includes a cleaning tank 2013, a mounting base plate 2014, and a cleaning base 2015. A channel mechanism 2020 is disposed within the cleaning tank 2013, and the upper part of the channel mechanism 2020 and the cleaning tank 2013 enclose a drainage cavity 2012. The mounting base plate 2014 is sealed to the bottom of the cleaning tank 2013, and the lower part of the channel mechanism 2020, together with the cleaning tank 2013 and the mounting base plate 2014, encloses a water inlet cavity 2011. The cleaning tank 2013 and the mounting base plate 2014 are both mounted on the cleaning base 2015.

[0509] In some embodiments, the mounting base plate 2014 is sealed to the cleaning tank 2013 via a sample cleaning sealing strip 20141.

[0510] In some embodiments, the channel mechanism 2020 and the cleaning tank 2013 are connected by a tight fit or adhesive bonding, or the channel mechanism 2020 and the cleaning tank 2013 can be integrally formed or detachably connected.

[0511] See Figure 42 In some embodiments, the sample pipetting device cleaning equipment 2000 further includes a second drainage mechanism 2050, which is installed on the tank mechanism 2010 and connected to the water inlet chamber 2011, for discharging the cleaning liquid in the water inlet chamber 2011 to prevent cross-contamination.

[0512] Specifically, after the cleaning operation is completed, cleaning fluid may remain on the inner wall of the inlet chamber 2011 and at the lower end of the sample transfer cleaning channel 2021. The second drainage mechanism 2050, directly connected to the inlet chamber 2011, can completely drain the residual cleaning fluid through gravity drainage or low-pressure suction, preventing the residual fluid from remaining in the inlet chamber 2011 or mixing with the newly injected clean cleaning fluid during the next cleaning. This cuts off the path for residual fluid to contaminate the new cleaning fluid at the source, ensuring that the initial environment for each cleaning is clean and meeting the cleanliness requirements of biological experiments and medical testing.

[0513] See Figure 42 In some embodiments, the bottom of the drainage chamber 2012 is inclined towards the first drainage mechanism 2040, referred to as the first inclined structure 2012a. The first inclined structure 2012a guides the cleaning fluid in the drainage chamber 2012 to be discharged from the first drainage mechanism 2040, facilitating drainage. Through the above arrangement, residual cleaning fluid in the drainage chamber 2012 is discharged, preventing cleaning fluid contamination. After the contaminated cleaning fluid in the sample transfer cleaning channel 2021 flows into the drainage chamber 2012 under gravity, the inclined structure guides the cleaning fluid to converge towards the interface of the first drainage mechanism 2040, preventing the cleaning fluid from accumulating at the bottom of the drainage chamber 2012.

[0514] See Figure 42 In some embodiments, the bottom of the water inlet chamber 2011 is inclined toward the second drainage mechanism 2050, referred to as the second inclined structure 2011a. The second inclined structure 2011a guides the cleaning fluid in the water inlet chamber 2011 to be discharged by the second drainage mechanism 2050, facilitating drainage. Through the above arrangement, residual cleaning fluid in the water inlet chamber 2011 is discharged, preventing cleaning fluid contamination.

[0515] It is understandable that the first inclined structure 2012a and the second inclined structure 2011a can be configured to change their inclination direction according to the different positions and numbers of the first drainage mechanism 2040 and the second drainage mechanism 2050. For example, when the first drainage mechanism 2040 is arranged on both sides of the drainage cavity 2012, the first inclined structure 2012a can be inclined from the middle of the drainage cavity 2012 to both sides.

[0516] In some embodiments, the first drainage mechanism 2040 is configured as a first drainage connector; and / or, the second drainage mechanism 2050 is configured as a second drainage connector.

[0517] See Figure 41 and Figure 42 In some embodiments, the sample pipetting device cleaning device 2000 further includes an overflow monitoring mechanism 2060, which is communicatively connected to the water inlet mechanism 2030 and is used to monitor the liquid level signal of the cleaning fluid in the sample pipetting device cleaning device 2000, which is denoted as the first liquid level signal; when the first liquid level signal is abnormal, a stop water inlet signal is sent to the water inlet mechanism 2030.

[0518] When the overflow monitoring agency detects an abnormality in the drainage, it stops the water intake.

[0519] Specifically, the overflow monitoring mechanism 2060 is configured as an overflow detection valve, which has a preset detection line. When the drain chamber 2012 is above the inlet chamber 2011, the overflow detection valve is located on the side wall of the drain chamber 2012. When an abnormal drainage occurs, the cleaning fluid in the drain chamber 2012 will increase. When the first liquid level signal in the drain chamber 2012 reaches the detection line of the overflow monitoring valve, the water intake is stopped to prevent the cleaning fluid from overflowing the tank mechanism 2010.

[0520] Through the above settings, the overflow monitoring mechanism 2060 monitors the first liquid level signal of the cleaning fluid in the tank mechanism 2010 and can respond quickly in case of drainage abnormalities, such as when the first drainage mechanism 2040 is blocked or the drainage rate lags behind the inlet rate. When the liquid level of the cleaning fluid in the drainage chamber 2012 above the inlet chamber 2011 rises to the preset detection line of the overflow detection valve due to poor drainage, the overflow monitoring mechanism 2060 immediately sends a stop inlet signal to the inlet mechanism 2030, cutting off the cleaning fluid supply at the source and preventing the cleaning fluid from accumulating and overflowing into the tank mechanism 2010, thus avoiding pollution of the surrounding environment or damage to other components. The communication connection between the overflow monitoring mechanism 2060 and the inlet mechanism 2030 forms a closed loop of monitoring and control, automatically triggering protective actions without manual intervention. On the one hand, it avoids potential safety hazards such as short circuits and component corrosion caused by overflowing cleaning fluid. On the other hand, timely shutdown of the supply reduces excessive cleaning fluid entering the drain chamber 2012, lowering the pressure on the drain chamber 2012 due to prolonged high liquid levels, extending the structural lifespan of the tank mechanism 2010, and reducing the frequency and cost of equipment maintenance caused by overflow. Furthermore, the overflow monitoring mechanism 2060 replaces traditional manual liquid level checks, providing 24-hour real-time monitoring of the liquid level, especially in high-throughput cleaning scenarios, preventing overflow problems caused by human negligence.

[0521] In some other embodiments, the water inlet chamber 2011 may be located above the drain chamber 2012, and the overflow detection valve may be located on the side wall of the water inlet chamber 2011. This ensures that the flow direction of the cleaning fluid matches its gravity direction, making the cleaning fluid in the sample transfer cleaning channel 2021 as fluid as possible, thereby improving the cleaning cleanliness.

[0522] See Figure 41 and Figure 42 In some embodiments, the sample pipetting device cleaning equipment 2000 further includes an ultrasonic cleaning transducer 2070, disposed on the tank mechanism 2010, for generating ultrasonic vibrations on the cleaning fluid in the sample transfer cleaning channel 2021. High-quality cleaning is achieved through the ultrasonic cleaning transducer 2070.

[0523] See Figure 45 In some embodiments, the sample pipetting device cleaning equipment 2000 further includes an automatic water inlet control mechanism 2080, which includes a liquid storage container 2081, a water replenishment component 2082, a water delivery component 2083, a liquid level monitoring component 2084, and a first controller. The liquid storage container 2081 stores the cleaning solution; the water replenishment component 2082 is connected to the liquid storage container 2081 and replenishes the liquid storage container 2081 with the cleaning solution; the water delivery component 2083 is connected between the liquid storage container 2081 and the water inlet mechanism 2030 and delivers the cleaning solution from the liquid storage container 2081 to the water inlet mechanism 2030; the liquid level monitoring component 2084 is disposed on the liquid storage container 2081 and monitors the liquid level signal of the cleaning solution in the liquid storage container 2081, which is denoted as the second liquid level signal; the first controller is communicatively connected to the liquid level detection mechanism, receives and controls the water replenishment component 2082 and the water delivery component 2083 to start and stop according to the second liquid level signal.

[0524] With the above setup, the automatic water inlet control mechanism 2080 stores cleaning fluid in the storage container 2081, and the liquid level monitoring component 2084 monitors the second liquid level signal in the storage container 2081 in real time. When the liquid level is lower than a preset threshold, the first controller can automatically control the water replenishment component 2082 to start, replenishing the cleaning fluid to the storage container 2081. This eliminates the need for frequent manual checks of the liquid level and manual water addition, making it particularly suitable for high-throughput cleaning scenarios, significantly reducing the frequency of manual intervention and simplifying operations. The water delivery component 2083 connects the storage container 2081 and the water inlet mechanism 2030, continuously supplying cleaning fluid to the water inlet mechanism 2030. Simultaneously, the coordination between the liquid level monitoring component 2084 and the first controller prevents the storage container 2081 from experiencing liquid interruption or overflow.

[0525] In some embodiments, the liquid storage container 2081 is provided with an inlet 20811, an outlet 20812, and a vent 20813. The water replenishment assembly 2082 includes an inlet pipe 20821 and a pressure reducing valve 20822 and an automatic water inlet valve 20823 disposed on the inlet pipe 20821. The pressure reducing valve 20822 stabilizes the pressure of the cleaning fluid input into the liquid storage container 2081. The inlet 20811 is connected to a water source via the inlet pipe 20821. The water delivery assembly 2083 includes an outlet pipe, and the outlet 20812 is connected to the inlet connector 2031 of the water inlet mechanism 2030 via the outlet pipe. The vent 20813 is disposed on the top of the liquid storage container 2081 and is used to communicate with the interior of the liquid storage container 2081 to maintain air pressure balance.

[0526] In some embodiments, the liquid level monitoring component 2084 is configured as a four-float sensor, installed on the top inner wall of the liquid storage container 2081 and extending downward. Specifically, the four-float sensor includes four float contacts arranged in series at intervals along the vertical direction. The four float contacts are located at different heights, and all float contacts are normally open contacts. They are conductive when immersed in the cleaning fluid and disconnected when not immersed in the cleaning fluid. The four float contacts are labeled L1-L4 from top to bottom.

[0527] Specifically, based on the different signal states of the four float contacts, the automatic water inlet control mechanism 2080 operates in the following ways:

[0528] When float contacts L1, L2, L3, and L4 are all in the conducting state, the automatic water intake mode is at the limit level. At this time, it is determined that there is a risk of overflow in the liquid storage container 2081 and an alarm is triggered. The automatic water intake control mechanism 2080 stops working to prevent the continuous replenishment of cleaning fluid from causing overflow.

[0529] When float contact L1 is in the open state and L2, L3, and L4 are in the closed state, the automatic water intake mode is in normal operation. The automatic water intake control mechanism 2080 continues to operate and simultaneously controls the water replenishment component 2082 to stop replenishing cleaning fluid to the liquid storage container 2081, maintaining the current liquid level to meet the water supply requirements.

[0530] When float contacts L1 and L2 are in the open state and L3 and L4 are in the closed state, the automatic water intake mode is in normal operation, and the automatic water intake control mechanism 2080 remains in operation. At this time, the liquid level in the liquid storage container 2081 is in the normal range, and there is no need to adjust the state of the water replenishment component 2082. The cleaning fluid is stably delivered to the water intake mechanism 2030 only through the water delivery component 2083.

[0531] When float contacts L1, L2, and L3 are in the open state and L4 is in the closed state, the automatic water intake mode is in normal operation. The automatic water intake control mechanism 2080 keeps running and simultaneously controls the water replenishment component 2082 to start, replenishing cleaning fluid into the liquid storage container 2081 to avoid the liquid level being too low and affecting subsequent liquid supply.

[0532] When the float contacts L1, L2, L3, and L4 are all in the open state, the automatic water intake mode is in the limited state. At this time, it is determined that there is no cleaning fluid in the liquid storage container 2081 and an alarm is triggered. The automatic water intake control mechanism 2080 stops to prevent the water supply component 2083 from running dry and being damaged.

[0533] When the float contact appears in any combination other than the five states mentioned above, the automatic water intake mode is set to the shutdown level. At this time, it is determined that the second liquid level signal is incorrect, which may be due to sensor failure or abnormal signal transmission. The automatic water intake control mechanism 2080 stops to avoid misoperation caused by signal errors and to ensure the safe operation of the equipment.

[0534] It is understood that the level monitoring component 2084 is not limited to four float sensors; it can be other numbers, more float sensors, or other types of components capable of level monitoring. The number of float sensors can be flexibly adjusted according to actual needs, further enhancing the adaptability of the automatic water inlet control mechanism 2080.

[0535] In some embodiments, the sample pipetting device cleaning equipment 2000 further includes a second controller, which is communicatively connected to the water inlet mechanism 2030 and the first drainage mechanism 2040 respectively; wherein, the second controller controls the water inlet mechanism 2030 and the first drainage mechanism 2040 to work synchronously, driving the cleaning fluid to form a flow state in the sample transfer cleaning channel 2021.

[0536] With the above settings, the flowing cleaning fluid can avoid the stagnation of cleaning fluid caused by the traditional method of water inlet followed by drainage. On the one hand, the contaminated cleaning fluid after use can be discharged in time and will not mix with the newly injected clean cleaning fluid, ensuring that the clean water that comes into contact with the parts to be cleaned is of high purity each time. On the other hand, the flowing cleaning fluid can create a flushing effect on the parts to be cleaned in the sample transfer cleaning channel 2021. Combined with the repeated suction and discharge action of the sample pipette tip 11120, it can more efficiently remove residual contaminants from the surface.

[0537] In addition, the synchronous control logic of the second controller can accurately match the inlet flow rate and the outlet flow rate. On the one hand, it prevents the inlet flow rate from exceeding the outlet flow rate, which would cause the liquid level in the outlet chamber 2012 to rise abnormally and avoid triggering the overflow monitoring mechanism 2060 to shut down frequently, thus ensuring the continuity of the cleaning process. On the other hand, it prevents the outlet flow rate from being too large, which would cause the pressure in the inlet chamber 2011 to be too low and prevent insufficient liquid supply to the sample transfer cleaning channel 2021. Synchronous control can ensure that the sample transfer cleaning channel 2021 in each independent area can obtain a stable liquid supply, avoid incomplete cleaning of some channels due to pressure imbalance, and ensure the consistency of the cleaning effect of multiple channels.

[0538] See Figure 27 and Figure 28 In some embodiments, the sample pipetting head module 1000 includes a specification recognition module 1500, which includes a recognition element 1510 and a sensing element 1520. The recognition element 1510 is disposed on the sample pipetting mechanism 1100, and the sensing element 1520 is disposed on the sample aspiration and dispensing driving mechanism 1200. The sensing element 1520 identifies the specification of the sample pipetting mechanism 1100 through the recognition element 1510.

[0539] The specification recognition module 1500 identifies the specifications of the sample pipetting mechanism 1100. After the sample pipetting mechanism 1100 is assembled onto the main body of the device via the pipetting tip quick-change device 1300, the recognition element 1510 and the sensing element 1520 enter the sensing range and form an effective signal connection. The pipetting device can automatically adjust its operating parameters according to the identified specifications of the sample pipetting mechanism 1100, eliminating the need for manual settings and improving pipetting accuracy and efficiency.

[0540] It is understood that the identification method of the identification element 1510 and the sensing element 1520 can be configured as contact identification and non-contact identification.

[0541] In some embodiments, the identification element 1510 includes a magnet, and the sensing element 1520 includes a Hall sensor. When the magnet is installed on the outer wall of the piston cavity 1111, the Hall sensor is installed on the device body at the position corresponding to the magnet. After the sample pipetting mechanism 1100 is installed on the device body, the magnet on the outer wall of the piston cavity 1111 enters the sensing area of ​​the Hall sensor. The sensor senses the change in magnetic field strength and then outputs an electrical signal corresponding to the magnetic field. The electronic control system of the device body will pre-store the specification data of the sample pipetting mechanism 1100 corresponding to different magnet configurations. After receiving the electrical signal from the sensor, the system matches it with the data in the database to automatically determine the specific specifications of the sample pipetting mechanism 1100 and synchronously adjust the operating parameters of the device.

[0542] Among them, the magnet and the Hall sensor have no mechanical contact, which is a non-contact identification method. It will not cause wear and jamming, can adapt to the high-frequency sample pipetting mechanism replacement scenario, extend the service life of the components, and has high identification stability and reliability.

[0543] In some embodiments, multiple magnets may be provided, which may have different shapes and arrangements in various positions. Different numbers, shapes and arrangements of magnets can be used to distinguish and identify sample pipetting mechanisms 1100 of different specifications.

[0544] In some embodiments, the identification element 1510 includes a micro switch, and the sensing element 1520 includes a mechanical triggering structure. When the micro switch is installed on the outer wall of the piston cavity 1111, the mechanical triggering structure is installed on the device body at the position corresponding to the micro switch. After the sample pipetting mechanism 1100 is installed on the device body, the mechanical triggering structure on the device body will make precise contact with the micro switch on the outer wall of the piston cavity 1111 and apply a preset mechanical pressure to change the circuit state of the micro switch, such as from open to closed, or from closed to open. The electronic control system of the device body will pre-store the specification data of the sample pipetting mechanism 1100 corresponding to different micro switch triggering states or triggering combinations. After receiving the on / off signal of the micro switch, the system will match it with the data in the database to automatically determine the specific specifications of the sample pipetting mechanism 1100 and adjust the operating parameters of the device synchronously.

[0545] Understandably, multiple microswitches can be configured with different shapes or different arrangements to distinguish between sample pipetting mechanisms 1100 of different specifications.

[0546] Among them, microswitches and mechanical triggering structures are contact-based identification methods, which provide stable and reliable signals, more accurate identification results, and low cost.

[0547] See Figures 46 to 51 In some embodiments, the sample transfer module 10000 further includes a sample pipetting frame module 5000, with the sample plate 0001 aspiration position, the reaction plate 0002 spray position, and the washing position disposed along the Y-axis on the sample pipetting frame module 5000; the sample pipetting arm 3000 includes a Y-axis drive assembly 3100 and a Z-axis drive assembly 3200 disposed at the output end of the Y-axis drive assembly 3100, the Y-axis drive assembly 3100 being mounted on the sample pipetting frame module 5000 and being used to drive the Z-axis drive assembly 3200 to move along the Y-axis direction; the output end of the Z-axis drive assembly 3200 is drivenly connected to the sample pipetting head module 1000 and is used to drive the sample pipetting head module 1000 to move along the Z-axis and Y-axis directions.

[0548] The sample pipetting frame module 5000 provides structural support, ensuring that other modules maintain high precision and stability during operation, reducing vibration and deformation, and protecting key internal components from the influence of the external environment.

[0549] See Figure 46 and Figure 47 In some embodiments, the sample pipetting frame module 5000 includes a frame assembly and an exterior assembly 5400. The frame assembly adopts a sheet metal bending and riveting structure and is equipped with a machined beam 5100, a tabletop 5120, a sample pipetting crossbeam 5200, and a sample pipetting upright beam 5300. Casters 5110 are provided at the bottom of the machined beam 5100. The sample pipetting crossbeam 5200 and the sample pipetting upright beam 5300 adopt a U-shaped or G-shaped structure to enhance rigidity and strength.

[0550] In some embodiments, the appearance component 5400 is mounted on the frame component to ensure high precision, stability, and protective performance of the equipment operation. The appearance component 5400 includes a sample pipetting top plate 5430, a sample pipetting side plate 5410, a sample pipetting rear plate 5420, and a sample pipetting front plate 5440. The sample pipetting front plate 5440 is equipped with a breathing light 5441c and a front door 5441, providing dustproof and safety protection functions. The components of the appearance component 5400 are mainly made of aluminum sheet metal, while the connectors and reinforcing corner pieces are machined from stainless steel. The entire component is installed onto the sample pipetting frame module 5000. The main exterior parts of the appearance component 5400 are powder-coated white, while the sample pipetting front plate 5440 is made of dark acrylic sheet, allowing users to visually observe the equipment's workflow. The front door 5441 is mounted on the sample pipetting front plate 5440 in a sliding manner, meeting the requirements of reasonable height, smooth switching operation, and reliable structure. The breathing light 5441c is configured as an RGB color breathing light strip to indicate the equipment's operating status. Specifically, the front door 5441 is made of black semi-transparent acrylic material. Its double-sided transparent area needs to be processed and protected, and the color separation lines need to be uniform and regular. The internal transparent area needs to be larger than the external transparent area, and both sides and the edges around the door are polished according to the appearance requirements and sprayed with black fine matte umbrella paint.

[0551] See Figure 48In some embodiments, the front door 5441 has a constant load spring 5441a mounted on a roller and a sample pipetting plate 5441d at one end. In use, the constant load spring 5441a, passing through the guide shaft on the roller, is used as one end, and the sample pipetting plate 5441d is used as the other end. Two constant load springs 5441a are fixed to the machined beam 5100 on one side and to the front door 5441 on the other. The weight of the front door 5441 is slightly greater than the load of the constant load springs 5441a. When the operator pushes the front door 5441 upwards, the front door 5441 slides along a preset trajectory. At this time, the constant load spring 5441a is stretched, generating an elastic force opposite to the weight of the front door 5441, offsetting part of the weight of the front door 5441, reducing pushing resistance, and achieving effortless sliding. After the front door 5441 slides to the preset height, it is locked and fixed by the latch 5441b structure, maintaining the open position. The spring remains stretched to balance the weight of the front door 5441 and prevent excessive force on the latch 5441b. After unlocking the latch 5441b, the front door 5441 slowly slides downward to reset under the combined action of its own weight and the elastic restoring force of the constant load spring 5441a, and the spring gradually contracts to its initial state.

[0552] Through the above configuration, the frame assembly provides stable structural support and comprehensive protection, reducing vibration and deformation during equipment operation. It also achieves dust prevention, safety protection, and equipment organization, meeting 5S management goals and resolving the issues of insufficient protection and cluttered appearance of existing equipment. The exterior component 5400 is fixed to the frame assembly with screws, enclosing non-display areas inside the equipment, concealing pipes, wiring, and other messy structures, while preventing dust from entering the equipment, forming a safety barrier.

[0553] See Figure 25 In some embodiments, the sample pipetting frame module 5000 further includes an illumination component 5500 disposed on the frame assembly for providing illumination.

[0554] See Figure 49 , Figure 50 and Figure 51 In some embodiments, the sample pipetting arm 3000 includes a Y-axis drive assembly 3100 comprising a Y-axis displacement unit 3110 and a Y-axis drive unit 3120. The Y-axis drive unit 3120 drives the Z-axis drive assembly 3200 to move along the horizontal Y-axis on the Y-axis displacement unit 3110, thereby enabling the sample pipetting head module 1000 to switch between the sample plate 0001 aspiration position, the reaction plate 0002 spray position, and the cleaning position, ensuring high-throughput pipetting efficiency and positioning accuracy.

[0555] Specifically, the Y-axis displacement unit 3110 includes two guide rail mounting plates 3111. The guide rail mounting plates 3111 serve as guiding references for Y-axis movement and are mounted on the machined beam 5100 of the frame assembly. A first guide rail 3112 is provided on the guide rail mounting plate 3111 along the Y-axis direction, and a first guide rail 3112 connecting plate is slidably connected to the first guide rail 3112. The Y-axis drive unit 3120 includes a synchronous belt drive element, which includes a Y-axis motor 3121, a Y-axis synchronous pulley 3122, a Y-axis synchronous belt 3123, a synchronous belt connecting plate 3124, and a cable connecting plate 3125. The Y-axis motor 3121 is driven by the Y-axis synchronous pulley 3122, which drives the Y-axis synchronous belt 3123 to rotate. The Y-axis synchronous belt 3123 is connected to the first guide rail 3112 connecting plate through the synchronous belt connecting plate 3124. The Z-axis drive assembly 3200 is fixedly connected to the first guide rail 3112 connecting plate.

[0556] The Y-axis drive assembly 3100 also includes a cable chain assembly 3130. The cable chain assembly 3130 includes a cable chain frame mounted on the sample pipetting frame module 5000, and a cable chain connected between the cable chain frame and the guide rail mounting plate 3111. The cable chain assembly 3130 is used to organize and transport motor cables, preventing them from becoming tangled or worn during movement. A timing belt connecting plate 3124 is screwed to the guide rail mounting plate 3111, and a cable connecting plate 3125 is screwed to the timing belt connecting plate 3124. The cable chain connecting plate is mounted to the guide rail mounting plate 3111 with screws, and the cable chain frame is fixed to the sample pipetting beam 5200 of the sample pipetting frame module 5000 with screws. Both ends of the cable chain are fixed to the cable chain connecting plate and the cable chain frame with screws, respectively.

[0557] With the above settings, the Y-axis motor 3121 drives the Y-axis synchronous belt 3123 to move through the Y-axis synchronous pulley 3122. The Y-axis synchronous belt 3123 transmits power to the guide rail mounting plate 3111, realizing the smooth horizontal movement of the Z-axis drive assembly 3200 and the sample pipetting head module 1000.

[0558] In some embodiments, the Z-axis drive assembly 3200 includes a Z-axis displacement unit 3210 and a Z-axis drive unit 3220. The Z-axis drive unit 3220 can drive the sample pipetting head module 1000 to move vertically along the Z-axis, thereby achieving vertical displacement for actions such as liquid aspiration, liquid spraying, head retraction, and cleaning.

[0559] Specifically, the Z-axis displacement unit 3210 includes a Z-axis mounting plate 3211 and a second guide mechanism. The Z-axis mounting plate 3211 fixes the Z-axis drive assembly 3200 onto the Y-axis drive assembly 3100, and the second guide mechanism ensures the verticality of the lifting motion. The second guide mechanism includes a second guide shaft 3212 and a second guide bearing 3213, with the second guide bearing 3213 mounted on the Z-axis mounting plate 3211. The second guide shaft 3212 is slidably connected within the second guide bearing 3213 and is connected to the sample pipetting head module 1000 by a guide shaft fixing plate 3212a.

[0560] The Z-axis drive unit 3220 includes a nut-screw transmission element 3221, a Z-axis synchronous pulley 3222, a Z-axis synchronous belt 3223, a Z-axis motor 3224, and a motor brake. The Z-axis motor 3224 drives the nut to rotate via the Z-axis synchronous belt 3223, thereby driving the screw to rise and fall. The Z-axis motor 3224 is configured as a stepper motor or a servo motor with an encoder. The Z-axis displacement unit 3210 also includes two upright plates 3214, which serve as mounting bases for the Z-axis drive assembly 3200 and connect the Z-axis mounting plate 3211 and the first guide rail 3112 connecting plate. The sample pipette module 1000 is fixedly connected to the guide shaft fixing plate 3212a of the Z-axis drive assembly 3200 via the pipette quick-change device 1300.

[0561] With the above configuration, the Z-axis motor 3224 outputs rotational power, which is transmitted to the nut via the Z-axis synchronous pulley 3222 and the Z-axis synchronous belt 3223. The rotation of the nut drives the screw to perform linear lifting and lowering motion, realizing the vertical displacement of the sample pipetting head module 1000. The encoder of the motor provides real-time feedback of the position signal, forming a closed-loop control and improving the Z-axis positioning accuracy. The Z-axis motor 3224 integrates a brake, which clamps the motor shaft when power is off, realizing Z-axis power-off self-locking and preventing the sample pipetting head module 1000 from falling and damaging the equipment or contaminating the sample. The lifting stroke of the Z-axis drive assembly 3200 is adapted to the vertical height difference of the sample plate 0001, reaction plate 0002, and cleaning tank 2013, ensuring that the sample pipetting head module 1000 can be smoothly inserted into the sample pipetting tip 11120, sample liquid, and cleaning liquid, while reserving space for the tip retraction movement. The nut and screw transmission element 3221 has a compact structure, transmits large torque, adapts to the load requirements of the 384-channel pipetting head, and the lifting and lowering movement is smooth and without swaying.

[0562] With the above settings, the sample pipette arm 3000 features fast movement speed, real-time and accurate position feedback, and high operational precision, which can effectively improve experimental efficiency and accuracy.

[0563] See Figures 52 to 55In some embodiments, the sample transfer module 10000 further includes a logistics transmission interaction module 6000, which includes a sample plate conveying mechanism 6100 and a reaction plate conveying mechanism 6200. The sample plate conveying mechanism 6100 is used to convey the sample plate 0001 to the sample plate 0001 liquid absorption position; the reaction plate conveying mechanism 6200 is used to convey the reaction plate 0002 to the reaction plate 0002 liquid spraying position or the next station.

[0564] See Figures 52 to 54 In some embodiments, the sample plate conveying mechanism 6100 includes a sample plate gripping assembly 6120 and a sample plate positioning assembly 6110. The sample plate gripping assembly 6120 includes a gripping drive member 6121 and a gripper 6122 drivenly connected to the gripping drive member 6121. The gripping drive member 6121 drives the gripper 6122 to move the sample plate 0001 to the liquid absorption position of the sample plate 0001. The sample plate positioning assembly 6110 includes a positioning side plate 6111 and an elastic side plate 6112. The elastic side plate 6112 is provided with a positioning elastic member 6113 that presses the sample plate 0001 against the positioning side plate 6111 to position the sample plate 0001 at the liquid absorption position of the sample plate 0001.

[0565] Specifically, there are two grippers 6122. Each gripper 6122 is equipped with a first gripping plate 6122a and a second gripping plate 6122b. The first gripping plate 6122a is L-shaped and is used to position one corner of the sample plate 0001. The second gripping plate 6122b is used to abut and position the sample plate 0001 at the edge away from the first gripper 6122. The first gripping plate 6122a and the second gripping plate 6122b of the two grippers 6122 together enclose and construct a gripping space. The sample plate 0001 is placed in the gripping space and can move with the grippers 6122.

[0566] In some embodiments, the gripping drive 6121 includes a gripping base plate 6121a, a gripping motor 6121b, a gripping timing pulley 6121c, a gripping timing belt 6121d, and a gripping slide rail 6121e. The gripping base plate 6121a is mounted on the sample pipetting frame module 5000. Both the gripping motor 6121b and the gripping slide rail 6121e are mounted on the gripping base plate 6121a. The output shaft of the gripping motor 6121b is driven by the gripping timing pulley 6121c, which drives the gripping timing belt 6121d to rotate. The gripper 6122 is fixedly connected to the gripping timing belt 6121d and slidably connected to the gripping slide rail 6121e.

[0567] With the above configuration, the gripping motor 6121b drives the gripping synchronous wheel 6121c to rotate, and the gripping synchronous wheel 6121c drives the gripper 6122 to move the sample plate 0001 to the liquid absorption position of the sample plate 0001 through the gripping synchronous belt 6121d.

[0568] In some embodiments, the ends of the positioning side plate 6111 and the elastic side plate 6112 of the sample plate positioning assembly 6110 are respectively provided with a first coarse positioning slope 6111a and a second coarse positioning slope 6112a. The first coarse positioning slope 6111a and the second coarse positioning slope 6112a form an open flared structure for providing coarse positioning guidance for the sample plate 0001 to enter between the positioning side plate 6111 and the elastic side plate 6112. The inner wall of the elastic side plate 6112 is provided with a positioning elastic element 6113, which includes a positioning bead 6113a and a positioning spring 6113b connected between the positioning bead 6113a and the elastic side plate 6112. Multiple positioning elastic elements 6113 may be provided. Under the elastic force of the positioning spring 6113b, the positioning bead 6113a is pressed against the side wall of the sample plate 0001, thereby pressing the sample plate 0001 against the positioning side plate 6111.

[0569] With the above settings, the sample plate positioning component 6110 can be adapted to sample plates 0001 with different sizes to achieve accurate positioning.

[0570] It is understood that in other embodiments, the sample plate positioning component 6110 may also be configured as a central positioning structure. Specifically, the sample plate positioning component 6110 includes two positioning side plates 6111, and the inner walls of the two positioning side plates 6111 are provided with positioning elastic members 6113. The positioning elastic members 6113 on both sides can abut the sample plate 0001 placed in the two positioning side plates 6111 at the central position.

[0571] In this embodiment, the positioning elastic element 6113 can be configured as a combination of positioning bead 6113a and positioning spring 6113b, or it can be directly configured as a flexible spring or a spring sheet structure, etc., which will not be described in detail here.

[0572] See Figure 55 In some embodiments, the reaction plate conveying mechanism 6200 includes a reaction plate positioning assembly 6210 and a reaction plate shaping assembly 6220. The reaction plate positioning assembly 6210 includes a reaction plate positioning member 6211, which is used to position the reaction plate 0002 at the liquid spraying position of the reaction plate 0002. The reaction plate shaping assembly 6220 includes a pressing plate 6221 and a pressing drive member 6222 driven by the pressing plate 6221. The pressing drive member 6222 drives the pressing plate 6221 to press against the upper surface of the reaction plate 0002 to shape its flatness. The reaction plate conveying mechanism 6200 also includes two reaction plate conveyor belts 6230 for conveying the reaction plate 0002.

[0573] In some embodiments, the reaction plate positioning member 6211 is configured as a reaction plate clamping arm. When the reaction plate conveying mechanism 6200 drives the reaction plate 0002 to the liquid spraying position, the reaction plate clamping arm rotates to clamp the reaction plate 0002. Two sets of pressure plates 6221 are provided, respectively placed on both sides above the pressure plates 6221. The pressure drive motor drives the pressure plates 6221 to press against the upper surface of the reaction plate 0002, shaping the flatness of the reaction plate 0002. After the plate making is completed, the reaction plate clamping arm rotates to reset, the pressure plates 6221 are lifted to reset, and the reaction plate 0002 can be conveyed to the next station by the reaction plate conveying mechanism 6200.

[0574] It is understandable that the reaction plate positioning component 6211 can also be configured as a positioning structure for other linear motion positioning components, capable of limiting and positioning the reaction plate 0002 at the liquid spray position. The shaping position and number of the clamping plate 6221 are variable. Its shaping position can be at the four corners of the reaction plate 0002 or at the middle position of the four edges of the reaction plate 0002; the number of clamping plates 6221 can be four or six. Alternatively, the clamping plate 6221 can be installed directly on the sample pipetting head module 1000 without a separate clamping drive component 6222, sharing the drive of the sample pipetting arm 3000 with the sample pipetting head module 1000.

[0575] See Figure 56 In some embodiments, the sample transfer module 10000 further includes a barcode scanning module 7000, which is located upstream of the liquid spraying position of the reaction plate 0002 and is used to record the barcode information on the reaction plate 0002 before liquid spraying.

[0576] In some embodiments, the barcode scanning module 7000 is mounted on the sample pipetting frame module 5000. Specifically, the barcode scanning module 7000 includes a fixed barcode reader 7100, a mounting bracket 7200, and a scanner connector 7300. The mounting bracket 7200 is mounted on the sample pipetting frame module 5000, and the fixed barcode reader 7100 is connected to the mounting bracket 7200 via the scanner connector 7300. The scanner connector 7300 is rotatably connected to the mounting bracket 7200 to adjust the scanning angle of the fixed barcode reader 7100. This allows for flexible adjustment of the scanning angle to accommodate differences in barcode position of reaction plates 0002 of different specifications, as well as slight placement deviations of the reaction plates 0002 in the logistics transmission interaction module 6000. This eliminates the need for frequent adjustments to the device or the placement of the reaction plates 0002, reducing operational complexity and improving scanning accuracy.

[0577] With the above setup, the barcode scanning module 7000 records the barcode information of reaction plate 0002 before spraying, binding the sample source, plate preparation parameters, and other data of each reaction plate 0002 to the barcode. Subsequently, the corresponding experimental data can be quickly retrieved via the barcode, effectively avoiding confusion of reaction plates 0002 in multiple batches of experiments. This meets the traceability requirements of biological experiments and medical testing, improving the reliability of experimental results. The barcode scanning module 7000 is located upstream of the spraying position of reaction plate 0002, completing the scanning process during the transport of reaction plate 0002 to the spraying position. This eliminates the need for an additional independent scanning step, does not occupy plate preparation time, ensures high-throughput plate preparation efficiency, and enhances the automation level of the sample transfer module 10000.

[0578] See Figure 57 , Figure 58 as well as Figure 60 In some embodiments, the reagent pipetting structure of the reagent adding module 20000 can be implemented in the following manner. In some embodiments, the liquid aspiration and dispensing of the reagent pipetting mechanism 20020 can be achieved using the same structure. The reagent pipetting shaft 20025 moves within the reagent pipetting chamber 20022, causing a change in the pressure within the reagent pipetting chamber 20022. When the pressure within the reagent pipetting chamber 20022 increases, the liquid within the reagent pipetting chamber 20022 is dispensed; when the pressure within the reagent pipetting chamber 20022 decreases, the liquid is drawn into the reagent pipetting chamber 20022.

[0579] In some embodiments, the reagent adding module 20000 further includes a reagent adding rack 20010, which is provided with a reagent adding station 20011 and a reagent aspiration station 20012. The reagent pipetting chamber 20022 and the reagent pipetting needle 20023 are both disposed on the reagent adding rack 20010. The reagent pipetting rack 20021 is movably disposed on the reagent adding rack 20010 to drive the reagent pipetting needle 20023 to move above the reagent adding station 20011 and above the reagent aspiration station 20012, respectively.

[0580] Combination Figure 60As shown, in some embodiments, the reagent pipetting structure includes a reagent pipetting air inlet 200221 disposed in the reagent pipetting chamber 20022, which is connected to a compressed air source. The reagent pipetting mechanism 20020 also includes a first valve 20024, which opens when the reagent pipetting needle 20023 sprays liquid. The two ends of the first valve 20024 are connected to the reagent pipetting air inlet 200221 and the reagent pipetting chamber 20022, respectively, and the compressed air source provides pressure to the reagent pipetting chamber 20022. Using the above design, compressed air is used to provide pressure to the reagent pipetting chamber 20022, thereby spraying the liquid out of the reagent pipetting chamber 20022.

[0581] In the embodiments of this application, when the reagent pipette 20023 passes over the reaction plate 0002 at high speed, it can spray the liquid in the reagent pipette chamber 20022 into the reaction plate 0002 in a very short time, approximately 3.2ms to 12ms.

[0582] It should be noted that the reagent plate can hold samples or reagents, and the reagent plate can be a different type of sample or reagent container, such as a 96-well plate, 384-well plate, or 1536-well plate that conforms to the SBS standard, or other homemade containers.

[0583] Combination Figure 60 As shown, the reagent pipetting mechanism 20020 also includes a reagent pipetting shaft 20025 and a reagent pipetting drive 20026. The reagent pipetting drive 20026 is drivenly connected to the reagent pipetting shaft 20025, so that the reagent pipetting shaft 20025 is movably inserted into the reagent pipetting chamber 20022. When the reagent pipetting needle 20023 draws liquid, the first valve 20024 is closed, and the reagent pipetting shaft 20025 moves away from the reagent pipetting needle 20023. With the above design, by closing the first valve 20024 and moving the reagent pipetting shaft 20025 away from the reagent pipetting shaft 20025, a negative pressure can be formed in the reagent pipetting chamber 20022, thereby drawing liquid into the reagent pipetting needle 20023.

[0584] Combination Figure 60 As shown, the reagent pipetting mechanism 20020 also includes a second valve 20027. When the second valve 20027 is open, its two ends are connected to the reagent pipetting needle 20023 and the reagent pipetting chamber 20022, respectively. By opening the second valve 20027, liquid can be drawn into or discharged from the reagent pipetting chamber 20022. By closing the second valve 20027, liquid can be retained within the reagent pipetting chamber 20022.

[0585] In some embodiments, there are multiple reagent pipettes 20023, first valves 20024, and second valves 20027. The reagent pipetting chamber 20022 is provided with multiple reagent pipetting channels, and the multiple reagent pipettes 20023 and multiple reagent pipetting channels are arranged in a one-to-one correspondence. Each reagent pipetting channel has a first valve 20024 and a second valve 20027 respectively located at both ends. With the above design, different liquids can be drawn using multiple reagent pipettes 20023 and reagent pipetting channels and poured into different wells of the reaction plate 0002, improving pipetting efficiency.

[0586] During liquid aspiration, multiple reagent pipettes 20023 can be powered by the reagent pipetting shaft 20025. Based on actual needs, the second valve 20027 corresponding to the reagent pipette 20023 requiring liquid aspiration is opened to draw the liquid into that specific reagent pipette 20023. When each reagent pipette 20023 operates independently, each reagent pipette 20023 should have its own corresponding reagent pipetting chamber 20022, allowing different liquids to be stored within each chamber when aspirating different liquids. Alternatively, if the reagent pipettes 20023 aspirate the same liquid, multiple reagent pipettes 20023 can share the same reagent pipetting chamber 20022.

[0587] Combination Figure 60 As shown, the reagent adding module 20000 also includes a matcher 20030, which is located in the reagent pipetting mechanism 20020 and used to match the information of the reaction plate 0002 and the reagent plate. With this design, when the reagent pipetting mechanism 20020 moves to the reagent adding station 20011 and the reagent aspiration station 20012, the matcher 20030 matches the information of the reaction plate 0002 and the reagent plate, thus confirming the information of the liquid in the container and avoiding incorrect liquid aspiration.

[0588] In some embodiments, the matcher 20030 may be a barcode scanner. Barcode information is provided on the reaction plate 0002 and the reagent plate. The barcode information is scanned by the barcode scanner to confirm whether the liquid in the reaction plate 0002 and the reagent plate meets the requirements.

[0589] Combination Figure 61As shown, the reagent adding module 20000 also includes a reagent adding drive mechanism 20040. The reagent adding drive mechanism 20040 includes a reagent beam 20041, a first reagent adding drive component 20042, a second reagent adding drive component 20043, and a third reagent adding drive component 20044. The first reagent adding drive component 20042 is drivenly connected to the reagent beam 20041 so that the reagent beam 20041 is movably disposed on the reagent adding frame 20010 along the Y-axis direction. The second reagent addition drive 20043 is disposed on the reagent beam 20041 and is driven to move the reagent pipette 20021 along the X-axis. The third reagent addition drive 20044 is disposed on the reagent pipette 20021 and is driven to move the reagent pipette 20023 along the Z-axis. The Y-axis, X-axis, and Z-axis are mutually perpendicular. The first reagent addition drive 20042 drives the reagent beam 20041 to move along the Y-axis, which in turn drives the second reagent addition drive 20043 to move along the Y-axis. The second reagent addition drive 20043 then drives the reagent pipette 20021 to move along the X-axis, which in turn drives the third reagent addition drive 20044 to move along the X-axis. Then, the reagent pipette 20023 is moved along the Z-axis by the third reagent addition drive 20044, thereby enabling the reagent pipette 20023 to move within the reagent addition frame 20010.

[0590] In some embodiments, the reagent dispensing rack 20010 is provided with a reagent dispensing longitudinal beam 20017, which extends along the Y-axis. A second reagent dispensing drive member 20043 is guided and engaged with the reagent dispensing longitudinal beam 20017, and the second reagent dispensing drive member 20043 is movable along the extension direction of the reagent dispensing longitudinal beam 20017. A reagent crossbeam 20041 extends along the X-axis, and a third reagent dispensing drive member 20044 is guided and engaged with the reagent crossbeam 20041.

[0591] Among them, the first reagent addition drive 20042, the second reagent addition drive 20043 and the third reagent addition drive 20044 are all in the form of motors.

[0592] Combination Figure 57 As shown, the reagent dispensing rack 20010 is equipped with multiple reagent aspiration stations 20012, which are located on both sides of the reagent dispensing station 20011 in the Y-axis direction. By setting multiple reagent aspiration stations 20012, multiple reagent plates can be placed simultaneously, enabling the reagent pipette 20023 to continuously pick up liquid and improve efficiency.

[0593] By distributing multiple reagent aspiration stations 20012 evenly on both sides of the reagent addition station 20011, the movement path of the reagent pipetting mechanism 20020 can be reduced, further improving the liquid collection efficiency.

[0594] Combination Figure 57 As shown, the reagent dispensing rack 20010 includes a reagent cleaning container placement position 20013. This position is located to one side of the reagent dispensing station 20011 along the Y-axis and is arranged sequentially with the reagent aspiration station 20012 along the X-axis, with the Y-axis and X-axis perpendicular to each other. The reagent pipette 20023 can move to a position above the reagent cleaning container placement position 20013. The reagent dispensing module 20000 also includes a reagent dispensing and cleaning pipeline and a reagent dispensing and cleaning element 20051. The reagent dispensing and cleaning element 20051 is connected to the cleaning container located at the reagent cleaning container placement position 20013 via the reagent dispensing and cleaning pipeline. Using this design, the cleaning solution is delivered to the cleaning container using the reagent dispensing and cleaning element 20051 and the reagent dispensing and cleaning pipeline. Then, the reagent pipette 20023 is moved to a position above the cleaning container, allowing it to be cleaned with the cleaning solution.

[0595] Combination Figure 57 and Figure 59 As shown, the reagent dispensing rack 20010 includes a reagent dispensing frame 20014, a reagent dispensing base plate 20015, a reagent dispensing interlayer plate 20016, a reagent dispensing longitudinal beam 20017, and a reagent dispensing top plate 20018. The reagent dispensing base plate 20015, reagent dispensing interlayer plate 20016, and reagent dispensing top plate 20018 are arranged sequentially along the Z-axis direction. The reagent dispensing longitudinal beam 20017 is located between the reagent dispensing interlayer plate 20016 and the reagent dispensing top plate 20018. Between these components, the reagent addition and cleaning pipeline and reagent addition and cleaning element 20051 are disposed on the reagent addition base plate 20015, the reagent addition station 20011, the reagent aspiration station 20012, and the reagent cleaning container placement position 20013 are disposed on the reagent addition interlayer plate 20016, the reagent pipetting mechanism 20020 is movably disposed on the reagent addition longitudinal beam 20017, and the reagent addition module 20000 also includes a control element disposed on the reagent addition top plate 20018. With the above design, the space in the Z-axis direction can be fully utilized to arrange the various components of the reagent addition module 20000, making the arrangement of each component more compact within the reagent addition rack 20010, saving space, and making the reagent addition module 20000 more miniaturized.

[0596] The control element is used to issue control commands to each execution element of the reagent addition module 20000. The execution elements include the first reagent addition driver 20042, the second reagent addition driver 20043, and the third reagent addition driver 20044 of this application.

[0597] Combination Figure 57 and Figure 58 As shown, the reagent dispensing rack 20010 includes a reagent dispensing frame 20014 and a reagent dispensing side plate 20019. The reagent dispensing side plate 20019 is located on one side of the reagent dispensing frame 20014 along the Y-axis. The reagent dispensing module 20000 also includes a reagent dispensing and cleaning pneumatic component 20052 and pneumatic tubing. The pneumatic tubing is connected to the reagent pipetting inlet 200221. The reagent dispensing and cleaning pneumatic component 20052 is disposed on the pneumatic tubing, and the reagent dispensing and cleaning pneumatic component 20052 and the pneumatic tubing are disposed on the reagent dispensing side plate 20019. This design fully utilizes the space along the Y-axis to arrange the pneumatic structure of the reagent pipetting mechanism 20020, making the components of the reagent dispensing module 20000 more compact, saving space, and making the reagent dispensing module 20000 more miniaturized.

[0598] Combination Figures 62 to 64 As shown, the reagent adding module 20000 also includes a cooling mechanism 20060, which includes a heat insulation component 20061 and a heat conduction component 20062. The heat insulation component 20061 includes a heat insulation module 200611 and an adapter 200612, which are attached together. The reagent aspiration station 20012 is located on the adapter 200612. The heat conduction component 20062 is separately disposed from the heat insulation component 20061, and the heat conduction component 20062 is connected to the heat insulation module 200611 through a pipeline. The heat conduction medium flows through the heat insulation module 200611 and the heat conduction component 20062 through the pipeline.

[0599] After the reagent plate containing the sample and / or reagent is placed at the reagent dispensing station 20012 of the adapter 200612, the insulation module 200611 provides cooling, which is then transferred to the adapter 200612, which is in contact with the insulation module 200611, thus providing cooling to the adapter 200612 and maintaining it at the required temperature. Simultaneously, the heat-conducting medium flowing in the heat-conducting component 20062 removes the heat generated by the cooling of the insulation module 200611, thereby cooling the insulation module 200611 and enabling it to operate normally.

[0600] Because the insulation component 20061 and the heat conduction component 20062 are set separately, and both have a small volume, the insulation component 20061 and the heat conduction component 20062 can be arranged separately within the reagent adding module 20000, making full use of the internal space of the reagent adding module 20000, and are not limited by the internal space of the equipment.

[0601] The insulation module 200611 is a thermoelectric cooler that operates using the Peltier effect. The thermoelectric cooler comprises two different semiconductors. When current flows through the two semiconductors, a cold end and a hot end are formed respectively. When the direction of the current in the two semiconductors is switched, the cold end and the hot end can switch between each other. This allows the insulation module 200611 to provide both cooling and heating to the adapter 200612, thereby maintaining the adapter 200612 within a certain temperature range.

[0602] Depending on actual needs, the cooling capacity provided by the thermoelectric cooler can be adjusted to keep the reagent in the well plate at around 5°C, with the temperature difference controlled within 3°C.

[0603] In some embodiments, the adapter 200612 is removable and replaceable, thereby adapting to different types of sample or reagent containers.

[0604] ...

Claims

1. A multifunctional sample reaction plate preparation platform, characterized in that, include: The sample transfer module includes a sample pipette tip module and a sample pipette arm. The sample pipette tip module includes a sample pipetting mechanism, a sample aspiration and dispensing drive mechanism, and a quick-change device for the pipette tip. The sample pipetting mechanism is detachably connected to the sample aspiration and dispensing drive mechanism via the quick-change device. The sample pipette arm is driven to the sample pipette tip module and is used to drive the sample pipette tip module to move to the sample transfer station to transfer the biological sample in the sample plate of the sample transfer station to the reaction plate of the sample transfer station. A reagent adding module includes a reagent pipetting mechanism, which comprises a reagent pipetting holder, a reagent pipetting chamber, and a reagent pipetting needle. The reagent pipetting needle and the reagent pipetting chamber are connected. The reagent pipetting chamber is provided with a reagent pipetting structure that provides pressure to the reagent pipetting chamber, causing the reaction reagent inside the chamber to be ejected through the reagent pipetting needle. The reagent pipetting holder is driven to the reagent pipetting needle and is used to drive the reagent pipetting needle to move to the reagent adding station to add the reaction reagent to the reaction plate at the reagent adding station. The drying module is provided with a drying preparation station located between the sample transfer station and the reagent addition station. The drying module is used to dry the biological samples in the sample plate of the drying preparation station and / or to dry the biological samples in the reaction plate of the drying preparation station. The heat sealing module is equipped with a heat sealing station located downstream of the reagent addition station, which is used to seal the reaction plate of the heat sealing station.

2. The multifunctional sample reaction plate preparation platform according to claim 1, characterized in that, The heat sealing module includes: The traction mechanism includes a heat-sealing motion frame that slides horizontally and a heat-sealing clamping assembly disposed on the heat-sealing motion frame. The heat-sealing clamping assembly clamps the heat-sealing film, and the heat-sealing motion frame is used to drive the heat-sealing clamping assembly to pull the heat-sealing film to move a preset length in the horizontal direction. The cutting mechanism includes a cutting linkage seat that slides horizontally and a cutting component disposed on the cutting linkage seat. The cutting component is used to cut a heat-sealing film of a preset length to obtain a heat-sealing film sheet. The heat-sealing mechanism includes a stamping component and a heat-sealing adsorption component; the heat-sealing adsorption component is used to grip the heat-sealing film; the stamping component is used to heat-seal the cut heat-sealing film onto the reaction plate.

3. The multifunctional sample reaction plate preparation platform according to claim 2, characterized in that, The traction mechanism further includes a hook assembly, which is slidably disposed between the heat-sealing motion frame and the cutting linkage seat; the hook assembly is used to pull the cutting assembly to the bottom end of the heat-sealing mechanism.

4. The multifunctional sample reaction plate preparation platform according to claim 3, characterized in that, The stamping assembly includes a stamping fixing plate, a stamping heating plate, and a stamping linkage plate arranged at intervals along the vertical direction; the stamping linkage plate is slidably disposed between the stamping fixing plate and the stamping heating plate; a plurality of first slide rods are installed on the stamping linkage plate, and a first heat-sealing elastic element is fitted on the first slide rod; the first heat-sealing elastic element is sandwiched between the stamping fixing plate and the stamping linkage plate; a suction cup is provided at the bottom end of each of the plurality of first slide rods; the suction cup is used to grasp the heat-sealing film.

5. The multifunctional sample reaction plate preparation platform according to claim 1, characterized in that, The drying module includes: A drying chamber is used for storing and drying consumables; a drying and loading mechanism includes a drying drive mechanism, wherein the drying drive mechanism includes a loading and loading sliding seat that is slidably arranged in the horizontal direction, and a loading and loading lifting part is provided on the side of the loading and loading sliding seat facing the drying chamber, the loading and loading lifting part is used to support the consumables, and the drying drive mechanism is used to drive the consumables to be loaded and loaded inside the drying chamber.

6. The multifunctional sample reaction plate preparation platform according to claim 5, characterized in that, The drying module includes: A drying and conveying mechanism is provided, which moves the consumables through the drying preparation station; the drying and conveying mechanism is connected between the sample transfer station and the reagent addition station, and is used to transport the sprayed reaction plate to the drying preparation station and / or the reagent addition station.

7. The multifunctional sample reaction plate preparation platform according to claim 6, characterized in that, The drying module also includes a drying lifting mechanism, which includes a drying lifting drive component, and the output end of the drying lifting drive component is provided with a drying support seat. The drying support is used to support the consumables, and the drying lifting drive is used to adjust the vertical height of the consumables so as to drive the consumables to detach from the drying transmission mechanism.

8. The multifunctional sample reaction plate preparation platform according to claim 1, characterized in that, The multifunctional sample reaction plate preparation platform also includes: A stack device is used to store consumables and to interact with the consumables in the sample transfer module, the reagent addition module, and the heat sealing module; the consumables include the sample plate and the reaction plate.

9. The multifunctional sample reaction plate preparation platform according to claim 8, characterized in that, The stacking device is at least partially connected to the sample transfer module for interacting with the sample transfer station on the sample plate and the reaction plate, so as to provide the sample plate and the reaction plate to the sample transfer station. And / or, the stacking device is at least partially connected to the reagent adding module for interacting with the reagent adding station to receive the reaction plate; And / or, the stacking device is at least partially connected to the heat sealing station for interacting with the heat sealing station to receive the reaction plate.

10. The multifunctional sample reaction plate preparation platform according to claim 8, characterized in that, The stack device includes: A hierarchical stack module includes several independent storage spaces, configured to independently access the consumables within any of the independent storage spaces; the consumables include sample boards; A stackable module, including stacked storage space, is configured to sequentially access consumables within the stacked storage space in a first-in-first-out (FIFO) order; the consumables include reaction plates.

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