Solvent effect separator

Abstract

The invention relates to the field of chromatographic columns, and particularly discloses a solvent effect separator which comprises a column body, and a plurality of crossed strip-shaped lines are arranged on the outer surface of the column body; the column body is divided into an upper connecting column body and a lower connecting column body, a liquid inlet is formed in the upper end of the upper connecting column body, and a liquid outlet is formed in the lower end of the lower connecting column body; according to the solvent effect separator, the solvent effect in a chromatographic column is effectively solved by additionally arranging the solvent effect separator at the front end of the chromatographic column, the structure is reasonable, repeated filtration is carried out through a filter plate and a separation plate, the disturbance caused by a sample solvent is solved, the peak shape is improved, the column efficiency is increased, and the separation degree is improved.

Description

Technical Field

[0001] This invention relates to the field of separation and filtration technology, and more specifically to a solvent effect separator. Background Technology

[0002] Chromatography is a widely used technique for separating, purifying, and analyzing compounds. The chromatographic column is one of the most important components of chromatography, enabling the separation of compounds into individual components. The separation efficiency of a chromatographic column depends on various factors, one of which is the solvent effect.

[0003] Traditional chromatographic columns typically require the use of multiple solvents for separation, but this method has several drawbacks. For example, the use of multiple solvents increases costs; complex solvent mixing is required, making operation difficult; and the high volatility of the solvents is environmentally unfriendly. Therefore, a more efficient separation technology is needed that can achieve separation by adding a solvent-effect separator at the front end of the chromatographic column, without the need for multiple solvents. Summary of the Invention

[0004] A method for preparing a solvent-effect separator is provided, which can achieve separation through the solvent effect without using multiple solvents. The solvent-effect separator solves the problem of efficient filtration. To achieve the above objective, the present invention adopts the following technical solution:

[0005] A solvent effect separator includes a column with a plurality of intersecting stripes on its outer surface; the column is divided into an upper connecting column and a lower connecting column, the upper connecting column having an inlet at its upper end and the lower connecting column having an outlet at its lower end.

[0006] The column is equipped with a solvent effect separation chamber; a spiral mixing chamber is provided on one side of the solvent effect separation chamber, and a separation chamber is provided on the other side, and the mixing chamber and the separation chamber are connected and interconnected.

[0007] The inlet of the mixing chamber is equipped with a filter plate, which is concave inward and has a protrusion at the bottom facing the interior of the mixing chamber. The filter plate is provided with several small mesh holes. The mixing chamber includes two mixing cavities and three spiral mixing grooves around the axis in each mixing cavity. The spiral mixing grooves are used to generate minute turbulence.

[0008] The liquid outlet of the separation chamber is equipped with a separation plate. The separation chamber includes two separation cavities and three spiral separation channels around the axis in each separation cavity. The spiral mixing channels are used to generate minute turbulence.

[0009] Both the inlet and outlet have slits on their outer walls.

[0010] The separating plate has several sieve holes running through it.

[0011] In summary, this application has the following beneficial effects:

[0012] This invention relates to a solvent effect separator. By adding a solvent effect separator to the front end of the chromatographic column, the solvent effect in the chromatographic column is effectively solved. Its structure is reasonable. Through multiple filtrations by the filter plate and the separation plate, the interference caused by "sample solvent" is eliminated, the peak shape is improved, the column efficiency is increased, and the resolution is improved. Attached Figure Description

[0013] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of the solvent effect separator of the present invention.

[0015] Figure 2 This is a cross-sectional view of the solvent effect separator of the present invention.

[0016] Figure 3 This is a cross-sectional view of the solvent effect separator of the present invention.

[0017] Figure 4 This is a schematic diagram of the filter structure of the solvent effect separator of the present invention.

[0018] Figure 5 This is a schematic diagram showing the mobile phase C filling the cavity during chromatographic equilibrium of the solvent effect separator of the present invention.

[0019] Figure 6 This is a schematic diagram illustrating how the sample solution H+X is efficiently mixed with the mobile phase C after injection in the solvent effect separator of this invention, resulting in a composition H+X+C that is closer to that of the mobile phase C.

[0020] Figure 7 This is a schematic diagram showing the highly efficient mixed sample solution after filtration by the solvent effect separator of the present invention reaching the chromatographic column.

[0021] Figure 8 This is a schematic diagram showing the efficient mixing of the sample solution and its partial entry into the chromatographic column using the solvent effect separator of the present invention.

[0022] Figure 9 This is a schematic diagram showing how the sample solution after efficient mixing is eluted by the mobile phase in the solvent effect separator of the present invention.

[0023] Figure 10 This is a schematic diagram illustrating the filtration and post-filtration detection of a sample according to the present invention.

[0024] The attached diagram lists the components represented by each number as follows:

[0025] 1-Column, 2-Striped pattern, 3-Upper connecting column, 4-Lower connecting column, 5-Inlet, 6-Outlet, 7-Solvent effect separation chamber, 8-Spiral mixing chamber, 9-Separation chamber, 10-Filter plate, 11-Protrusion, 12-Mesh, 13-Sieve hole, 14-Separation plate, 81-Mixing cavity, 82-Spiral mixing tank, 91-Separation plate, 92-Separation cavity, 93-Spiral separation tank. Detailed Implementation

[0026] The present application will be further described in detail below with reference to the embodiments.

[0027] Example:

[0028] A solvent effect separator includes a column 1, the outer surface of which is provided with a plurality of intersecting stripes 2; the column 1 is divided into an upper connecting column 3 and a lower connecting column 4, the upper end of the upper connecting column 3 is provided with a liquid inlet 5, and the lower end of the lower connecting column 4 is provided with a liquid outlet 6.

[0029] The column 1 is provided with a solvent effect separation chamber 7 inside; a mixing chamber 8 is provided on one side of the solvent effect separation chamber 7 and a separation chamber 9 is provided on the other side, and the spiral mixing chamber 8 and the separation chamber 9 are connected and communicate with each other;

[0030] The inlet of the mixing chamber 8 is provided with a filter plate 10, which is recessed inward and has a protrusion 11 at the bottom. The protrusion 11 faces inward towards the inside of the mixing chamber 8. The filter plate 10 is provided with a number of small mesh holes 12. The mixing chamber 8 includes two mixing cavities 81 and three spiral mixing grooves 82 arranged around the axis in each mixing cavity 81. The spiral mixing grooves 82 are used to generate minute turbulence.

[0031] The separation chamber 9 is equipped with a separation plate at its outlet. The separation chamber 9 includes two separation cavities 92 and three spiral separation channels 93 arranged around an axis in each separation cavity 92. The spiral mixing channels 82 are used to generate minute turbulence. The outer walls of the inlet 5 and the outlet 6 are provided with slits.

[0032] The separating plate has several sieve holes running through it.

[0033] The specific working principle of the solvent effect separator of this invention is as follows: Figure 1 As shown, firstly, external liquid enters the inlet 5 of this invention, and then sequentially enters the solvent effect separation chamber 7 until it flows out from the outlet 6.

[0034] The device of this invention is installed at the front end of the chromatographic column. Its structure accelerates the separation of sample molecules and solvent molecules, avoiding the solvent effect (if the solvent H used to dissolve the sample is not compatible with the mobile phase C, and the sample is prematurely eluted before fully entering a good pre-separation state, a solvent effect that causes strange peak shapes will occur). For the specific filtration process, see [link to filtration process]. Figure 4 As shown in the figure, the liquid enters the chromatographic column through the solvent effect separator.

[0035] Initially, the mobile phase C fills the cavity. Then, sample molecules X and solvent molecules H are propelled by the mobile phase C to reach the chromatographic column. The sample molecules are eluted by the mobile phase, and sample molecules X and solvent molecules H are separated. Sample molecules X enter the chromatographic column for retention and detection.

[0036] The injection process is illustrated as follows: In the first stage, the environment surrounding the sample molecules changes from "solvent molecules H" to "a mixture of solvent molecules and mobile phase molecules H+C", and then rapidly transforms into "mobile phase molecules C". However, with a solvent effect eliminator connected before the column, the sample solution H+X mixes efficiently with the mobile phase C in its solvent effect separation chamber 7. Before the sample molecule X reaches the column, its surrounding environment changes from "solvent molecules H" to "a mixture of solvent molecules and mobile phase molecules H+C". When the sample molecule X enters the column and begins its chromatographic retention behavior, the change in its surrounding environment is not as drastic as without the solvent effect eliminator; its surrounding environment merely changes from "a mixture of solvent molecules and mobile phase molecules H+C" to "mobile phase molecules C", allowing the sample molecule X to enter the pre-separation state earlier.

[0037] In the second process, sample molecule X interacts with the C18 long chains of the packing material inside the chromatographic column. Before contact with the C18 long chains, sample molecule X is surrounded by solvent molecules H, while the C18 long chains are surrounded by mobile phase molecules C. If the sample solvent H and mobile phase C are too different or incompatible, the contact between sample molecule X and the C18 long chains will not be as smooth as expected, and there will be a process of mutual fusion. However, after adding a solvent effect eliminator before the chromatographic column, the sample solution H+X and mobile phase C mix efficiently in its solvent effect separation chamber 7. Before sample molecule X reaches the chromatographic column, its surrounding environment is changed from "solvent molecules H" to "a mixture of solvent molecules and mobile phase molecules H+C". After sample molecule X enters the chromatographic column, the number of mobile phase molecules C and solvent molecules H in the surrounding environment increases, making it closer to the C18 long chains in the same environment. The resistance to contact decreases, the contact between sample molecule X and the C18 long chains is smoother, and the pre-separation state for formal chromatographic retention behavior is faster.

[0038] In the third process, the "sample solution H+X that did not enter the column (the concentration of the sample solution is usually very small, so the main component is the sample solvent H)" becomes the mobile phase of "sample molecules X1 that first enter the column" in a local area. At this time, the composition of H+X (mainly H) as the temporary mobile phase is quite different from the composition of the formal mobile phase C.

[0039] After adding a solvent effect eliminator before the column, the sample solution H+X mixes efficiently with the mobile phase C in its cavity. The portion of the sample solution H+X that does not enter the column (mainly H) becomes a mixture of sample molecules, solvent molecules, and mobile phase molecules, H+X+C (where X is very small and negligible, mainly H+C). Its composition is closer to that of the mobile phase C. Therefore, the function of adding a solvent effect eliminator before the column is to reduce the difference between the sample solution H+X as a transient mobile phase and the formal mobile phase C. The composition of the sample solution as a transient, localized mobile phase is optimized, making the sample molecules X1 that enter the column first more like the retention behavior of entering the formal chromatogram, being eluted by the mobile phase molecules C, rather than by the sample solution H+X, which has a much different property from the mobile phase. Based on the analysis of the above injection process, the solvent effect eliminator has a good improvement effect on all three processes in the injection process. It enables the sample to complete the compatibility problem between the sample and the mobile phase before reaching the chromatographic column, and enter the pre-separation state in advance. After the sample molecules reach the chromatographic column, they can be eluted by the mobile phase in the best state, thereby eliminating the solvent effect.

[0040] This invention Figure 10 Spectrum analysis for a solvent effect eliminator – for a specific sample.

[0041] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A solvent effect separator, comprising a column having a plurality of intersecting stripes on its outer surface; the column being divided into an upper connecting column and a lower connecting column, the upper connecting column having an inlet at its upper end and the lower connecting column having an outlet at its lower end; characterized in that The column is equipped with a solvent effect separation chamber; a spiral mixing chamber is provided on one side of the solvent effect separation chamber, and a separation chamber is provided on the other side, and the mixing chamber and the separation chamber are connected and interconnected. The inlet of the mixing chamber is equipped with a filter plate, which is concave inward and has a protrusion at the bottom facing the interior of the mixing chamber. The filter plate has several small mesh holes. The mixing chamber includes two mixing cavities and three spiral mixing grooves around the axis in each mixing cavity. The spiral mixing grooves are used to generate minute turbulence.

2. A solvent effect separator according to claim 1, wherein The liquid outlet of the separation chamber is equipped with a separation plate. The separation chamber includes two separation cavities and three spiral separation grooves arranged around the axis in each separation cavity. The spiral mixing grooves are used to generate minute turbulence.

3. A solvent effect separator according to claim 1, wherein Both the inlet and outlet have slits on their outer walls.

4. A solvent effect separator according to claim 1, wherein The separating plate has several sieve holes running through it.

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