Janus hydrogel based on fish oil and AgNP
Janus hydrogels based on fish oil and AgNP were prepared in a one-step process, which solved the problems of material complexity and poor biocompatibility in the prior art. This process achieved asymmetric adhesion and improved conductivity, making it suitable for medical adhesives.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE SIXTH MEDICAL CENT OF THE CHINESE PEOPLES LIBERATION ARMY GENERAL HOSPITAL
- Filing Date
- 2025-11-25
- Publication Date
- 2026-04-17
AI Technical Summary
Existing Janus hydrogel materials are complex, have complicated preparation processes, and some components have poor biocompatibility or are toxic, affecting their performance.
Janus hydrogel based on fish oil and AgNP was prepared by a one-step method. Silver nanoparticles were uniformly suspended in deionized water, and a specific ratio of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, acrylic acid and DHA were added. After stirring, an initiator and a crosslinking agent were added. After crosslinking, the mixture was purified by immersion in deionized water to obtain purified MAH hydrogel.
A hydrogel with asymmetric adhesion was obtained without secondary modification, which improved biocompatibility and enhanced conductivity, making it suitable for myocardial repair.
Smart Images
Figure CN121873384A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of hydrogel technology, and more particularly to a Janus hydrogel based on fish oil and AgNP. Background Technology
[0002] Existing Janus hydrogel technologies mostly employ a two-step bonding method to create an adhesive layer on one side and a non-adhesive layer on the other. This involves first forming an adhesive hydrogel through chemical bonds such as hydrogen bonds between different substances, followed by the addition of an anti-adhesion hydrogel layer to the top surface, thus achieving anti-adhesion properties. The bonding of the two hydrogels results in asymmetric adhesion of the final Janus hydrogel. Existing technologies have the following drawbacks: ① Complex materials and complicated preparation processes; ② Some components have poor biocompatibility or are even toxic to organisms, and toxicity can only be removed through immersion, but the performance is significantly reduced during immersion.
[0003] Therefore, there is an urgent need to provide a technical solution that addresses the shortcomings of the existing technology. Summary of the Invention
[0004] The purpose of this application is to provide a Janus hydrogel based on fish oil and AgNP to solve or alleviate the problems existing in the prior art.
[0005] To achieve the above objectives, this application provides the following technical solution: A method for preparing Janus hydrogel based on fish oil and AgNP includes the following steps: (1) Silver nanoparticles were uniformly suspended in deionized water, and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, acrylic acid and DHA were added. After stirring, an initiator and a crosslinking agent were added and stirring was continued to obtain a hydrogel precursor. (2) The hydrogel precursor solution was added to a PTFE mold and crosslinked to obtain a hydrogel; (3) The hydrogel was purified by immersing it in deionized water and placed in a fume hood at room temperature to obtain purified MAH hydrogel.
[0006] Preferably, the molar ratio of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide to acrylic acid is 1:4 to 1:6.
[0007] Preferably, the mass ratio of the silver nanoparticles to the hydrogel precursor is 6%wt, and the particle size of the silver nanoparticles is 20nm.
[0008] Preferably, the mass ratio of DHA to the hydrogel precursor is 6%wt.
[0009] Preferably, the initiator is ammonium persulfate, and the molar ratio of the initiator to [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide is 1:200 to 1:250; the mass ratio of N,N'-methylenebisacrylamide to the hydrogel precursor is 0.04%wt.
[0010] Preferably, the crosslinking agent is N,N'-methylenebisacrylamide, and the molar ratio of the crosslinking agent to [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide is 1:200 to 1:300.
[0011] More preferably, the method for preparing Janus hydrogel based on fish oil and AgNP includes the following steps: (1) Silver nanoparticles were uniformly suspended in deionized water at 800 rpm, and then [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, acrylic acid and DHA were added. The mixture was stirred at 800 rpm for 4 h and the total mass of the mixture was controlled at 10 g. Initiator and crosslinking agent were added and stirred continuously to form hydrogel precursor. (2) The hydrogel precursor solution was added into a PTFE mold covered by a glass plate and crosslinked at 70°C for 6 h to obtain a hydrogel; (3) Immerse the hydrogel in deionized water and repeat several times to remove residual reagents and obtain purified hydrogel. Place it in a fume hood at room temperature to obtain purified MAH hydrogel until the original weight is reached.
[0012] A Janus hydrogel based on fish oil and AgNP was prepared by the above-mentioned method to obtain MAH hydrogel.
[0013] An application of the Janus hydrogel based on fish oil and AgNP as described above, wherein the MAH hydrogel is used in the preparation of medical adhesives.
[0014] Beneficial effects: (1) The present invention prepares Janus hydrogel (MAH hydrogel) in one step without secondary modification to obtain a hydrogel with asymmetric adhesion.
[0015] (2) The oil phase material of the present invention is fish oil component that is beneficial to the heart (especially docosahexaenoic acid in fish oil, abbreviated as DHA, commonly known as brain gold), which greatly improves biocompatibility.
[0016] (3) The present invention incorporates silver nanoparticles to improve the conductivity of Janus hydrogel material, providing better conditions for myocardial repair. Attached Figure Description
[0017] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. Wherein: Figure 1 The adhesive properties of the MAH hydrogel in Example 1 were characterized. Figure 2 Biocompatibility characterization of the MAH hydrogel in Example 1; Figure 3 The conductivity of the MAH hydrogel in Example 1 is characterized. Detailed Implementation
[0018] The present application will now be described in detail with reference to the accompanying drawings and embodiments. Various examples are provided by way of explanation and not by way of limitation. In fact, those skilled in the art will understand that modifications and variations can be made to the present application without departing from the scope or spirit of the present application. For example, a feature shown or described as part of one embodiment may be used in another embodiment to produce yet another embodiment. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention should fall within the scope of protection of the embodiments of the present invention.
[0019] Example 1 A method for preparing Janus hydrogel based on fish oil and AgNP includes the following steps: (1) First, silver nanoparticles (0.63 g) were uniformly suspended in 5 mL of deionized water at 800 rpm. DMAPS ([2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, 1.94 g, 6.9 mmol, CAS No. 3637-26-1), AA (acrylic acid, 2.5 g, 34.7 mmol) and DHA (docosahexaenoic acid, 0.6 g, 1.8 mmol) were added sequentially. The mixture was stirred at a certain stirring speed (800 rpm) for 4 h, and the total mass of the mixture was controlled to be 10 g. Initiator APS (ammonium persulfate, 0.0069 g, 0.03 mmol) and crosslinking agent MBA (N,N′-methylenebisacrylamide, 0.004 g, 0.026 mmol) were added and stirred continuously for 5 min to form a hydrogel precursor.
[0020] (2) The hydrogel precursor solution formed in step (1) is added into a PTFE mold (25 mm long, 20 mm wide, and 1.5 mm thick) covered by a glass sheet and crosslinked at 70°C for 6 h to obtain a hydrogel.
[0021] (3) Immerse the hydrogel from step (2) in deionized water (500 mL, 2 h, repeated 6 times) to thoroughly purify the hydrogel to remove residual reagents in the hydrogel; and obtain purified MAH hydrogel until the original weight is reached by placing it in a fume hood at room temperature.
[0022] Comparative Example 1 Unlike Example 1, DHA (docosahexaenoic acid, 0.6 g, 1.8 mmol) was replaced with SMA (octadecyl methacrylate, 0.62 g, 1.8 mmol).
[0023] The MAH hydrogel prepared in this embodiment was characterized, and the results are as follows: Figure 1-3 As shown.
[0024] Figure 1 This is a characterization image of the two-sided adhesion test performed on the Janus asymmetric hydrogel (the MAH hydrogel prepared in this example). Figure 1 It can be seen that, with different proportions of the oil phase (2%, 4%, 6%, 8%, 10%), the adhesion of both sides first increases and then decreases, with the strongest adhesion at a concentration of 6%. Furthermore, the adhesion of the top and bottom layers differs at all concentrations.
[0025] Figure 2 When the materials used are fish oil's basic component DHA and a similar structure SMA (octadecyl methacrylate, comparative example 1), biocompatibility live / dead assays are performed. Figure 2 It can be seen that SMA has a large number of dead cells at 7 days. Figure 2 As shown in red in the middle), while DHA ( Figure 2 As shown in green in the middle (left and right), there are almost no dead cells, indicating that using DHA, a basic component of fish oil, as the oil phase of the material greatly improves the biocompatibility of the hydrogel material.
[0026] Figure 3 To determine the intracellular calcium ion concentration when silver nanoparticles and the non-conductive surfactant CTAB are used as the material, this study aims to assess the strength of the cell's electrical conductivity. Figure 3 It can be seen that when silver nanoparticles are used as the material, the concentration of calcium ions in the cells increases, indicating that the material promotes electrical conduction between cardiomyocytes.
[0027] In this invention, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0028] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.
Claims
1. A method for preparing a Janus hydrogel based on fish oil and AgNPs, characterized by, Includes the following steps: (1) Silver nanoparticles were uniformly suspended in deionized water, and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, acrylic acid and DHA were added. After stirring, an initiator and a crosslinking agent were added and stirring was continued to obtain a hydrogel precursor. (2) The hydrogel precursor solution was added to a PTFE mold and crosslinked to obtain a hydrogel; (3) The hydrogel was purified by immersing it in deionized water and placed in a fume hood at room temperature to obtain purified MAH hydrogel.
2. The method for preparing Janus hydrogel based on fish oil and AgNP according to claim 1, characterized in that, The molar ratio of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide to acrylic acid is 1:4 to 1:
6.
3. The method for preparing Janus hydrogel based on fish oil and AgNP according to claim 1, characterized in that, The mass ratio of the silver nanoparticles to the hydrogel precursor is 6%wt, and the particle size of the silver nanoparticles is 20nm.
4. The method for preparing Janus hydrogel based on fish oil and AgNP according to claim 1, characterized in that, The mass ratio of DHA to the hydrogel precursor is 6%wt.
5. The method for preparing Janus hydrogel based on fish oil and AgNP according to claim 1, characterized in that, The initiator is ammonium persulfate, and the molar ratio of the initiator to [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide is 1:200 to 1:250; the mass ratio of N,N'-methylenebisacrylamide to the hydrogel precursor is 0.04%wt.
6. The method for preparing Janus hydrogel based on fish oil and AgNP according to claim 5, characterized in that, The crosslinking agent is N,N'-methylenebisacrylamide, and the molar ratio of the crosslinking agent to [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide is 1:200 to 1:
300.
7. The method for preparing Janus hydrogel based on fish oil and AgNP according to any one of claims 1 to 6, characterized in that, Includes the following steps: (1) Silver nanoparticles were uniformly suspended in deionized water at 800 rpm, and then [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, acrylic acid and DHA were added. The mixture was stirred at 800 rpm for 4 h and the total mass of the mixture was controlled at 10 g. Initiator and crosslinking agent were added and stirred continuously to form hydrogel precursor. (2) The hydrogel precursor solution was added into a PTFE mold covered by a glass plate and crosslinked at 70°C for 6 h to obtain a hydrogel; (3) Immerse the hydrogel in deionized water repeatedly to remove residual reagents and obtain purified hydrogel. Place it in a fume hood at room temperature to obtain purified MAH hydrogel until the original weight is reached.
8. A Janus hydrogel based on fish oil and AgNP, characterized in that, MAH hydrogels were prepared by the preparation method according to any one of claims 1 to 6.
9. An application of the Janus hydrogel based on fish oil and AgNP as described in claim 8, wherein the MAH hydrogel is used in the preparation of medical adhesives.