Jellyfish fatty acid liposome, preparation method thereof and application of jellyfish fatty acid liposome in liquid dressing

Abstract

The invention relates to jellyfish fatty acid liposome as well as a preparation method and application thereof in liquid dressings, and belongs to the technical field of preparation of liquid dressings. The preparation method of the jellyfish fatty acid liposome comprises the following steps: (1) extracting jellyfish fatty acid; (2) preparing a jellyfish fatty acid lipid solution; (3) preparing jellyfish fatty acid lipid colostrum; and (4) preparing the jellyfish fatty acid liposome. The invention also provides a liquid dressing containing the jellyfish fatty acid liposome. The lipidosome is used as a carrier to bear the jellyfish fatty acid, so that the stability of the jellyfish fatty acid is greatly improved, the degradation rate of active ingredients is less than 10% under the condition of 25 DEG C / 6 months, and the activity loss in the preparation and storage processes is greatly reduced. And the transdermal absorption rate (90%) of jellyfish fatty acid can be remarkably improved, so that the effective components act on the target part more efficiently, and the actual use effect of the product is enhanced.

Description

Technical Field

[0001] This invention relates to a jellyfish fatty acid liposome, its preparation method, and its application in liquid dressings, belonging to the field of liquid dressing preparation technology. Background Technology

[0002] Liquid dressings are widely used in skin care, cosmetic repair, and wound healing. Common liquid dressings often use plant extracts or synthetic active ingredients, such as hyaluronic acid and collagen. However, they suffer from problems such as poor stability of active ingredients, low transdermal absorption, and susceptibility to oxidation and degradation. Especially in carriers such as freeze-dried crystal masks, the essence often fails to fully release from the substrate, leading to insufficient skin absorption and affecting the effectiveness of the treatment.

[0003] While there are reports of using liposomes as carriers to improve the stability of liquid dressing ingredients, these are mostly limited to common fatty acids from plant or animal sources. Their antibacterial and antioxidant activities are limited, and they lack specific ability to promote skin barrier repair and collagen regeneration. In other words, although existing liquid dressings possess some antioxidant properties, they have not solved the core problems of poor stability of active ingredients and low transdermal efficiency.

[0004] Fatty acids are important organic compounds widely found in humans and other animals, playing a vital role in energy supply and structural composition during metabolism. They have been widely used in the preparation of various dressing products. Jellyfish, as an abundant marine resource, contain almost no carbohydrates, fats, or proteins, but are rich in uniquely composed fatty acids, with unsaturated fatty acids accounting for 42.9% to 54.7%. These jellyfish fatty acids exhibit significantly superior antibacterial, anti-inflammatory, and antioxidant activities compared to traditional plant fatty acids, demonstrating significant biological activity. However, due to the difficulty of extraction and poor stability, they have not yet been effectively utilized in liquid dressings.

[0005] Therefore, developing a liposome carrier based on jellyfish fatty acids that combines high stability, high permeability, and multiple bioactive properties into a liquid dressing can significantly compensate for the current shortcomings in the field of liquid dressings. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a jellyfish fatty acid liposome, its preparation method, and its application in liquid dressings, thereby solving the problem that existing facial mask essence tends to remain on the mask substrate during the use of freeze-dried crystal masks, making it difficult for the face to absorb.

[0007] The technical solution of the present invention is as follows: A method for preparing jellyfish fatty acid liposomes includes the following steps: (1) Jellyfish fatty acids were obtained by crushing, extracting with organic solvents, purifying and freeze-drying the jellyfish. (2) Jellyfish fatty acids, soybean lecithin and cholesterol are added sequentially to a chloroform-methanol mixed solvent to obtain a jellyfish fatty acid lipid solution; (3) The jellyfish fatty acid lipid solution was prepared into a jellyfish fatty acid lipid film by thin film dispersion method, and then the jellyfish fatty acid lipid film was redissolved and subjected to water bath sonication to obtain jellyfish fatty acid lipid colostrum; (4) Jellyfish fatty acid lipid colostrum was subjected to high pressure homogenization and freeze drying to obtain jellyfish fatty acid liposomes.

[0008] According to a preferred embodiment of the present invention, in step (1), the jellyfish is a moon jellyfish or a jellyfish.

[0009] According to a preferred embodiment of the present invention, the specific steps of pulverization, extraction, purification and freeze-drying in step (1) are as follows: S1: Select fresh jellyfish as the extraction raw material, remove the jellyfish's internal organs and other impurities, then store the cleaned jellyfish at -35~-45℃ for 20~30h, and then pulverize it to 180~220 mesh to obtain jellyfish powder; S2: Add anhydrous ethanol to the jellyfish powder at a material-to-liquid ratio of 1:10, and extract ultrasonically at 55-65℃, 200-300W, and 40kHz for 1.5-2.5h. Centrifuge the resulting mixture at 7500-8500rpm for 12-18min and collect the supernatant. Repeat the ultrasonic extraction and centrifugation 2-3 times, and combine all the supernatants to obtain the jellyfish extract. S3: The jellyfish extract was rotary evaporated at 45~55℃ and 0.05~0.1MPa until the anhydrous ethanol was completely evaporated to obtain crude jellyfish fatty acid extract; S4: Dissolve the crude fatty acid extract of jellyfish in ultrapure water, add n-hexane at a volume ratio of 1:3 to obtain the extraction system; shake the extraction system at 150~200 rpm for 10~15 min, then let it stand until the layers are separated, and take the aqueous phase; repeat the shaking extraction and standing for a total of 2~3 times, combine all the aqueous phases to obtain the jellyfish fatty acid extract; S5. Jellyfish fatty acid extract was freeze-dried at -45~-55℃ for 45~50h to obtain jellyfish fatty acid.

[0010] According to a preferred embodiment of the present invention, in step (2), the mass-volume ratio of the jellyfish fatty acid, soybean phospholipid, cholesterol, and chloroform-methanol mixed solvent is (15~25):(80~120):(15~25):(10~20), unit: mg / mg / mg / mL.

[0011] According to a preferred embodiment of the present invention, in step (2), the chloroform-methanol mixed solvent is prepared by mixing chloroform and methanol in a volume ratio of 2:1.

[0012] According to a preferred embodiment of the present invention, in step (3), the thin film dispersion method, resolution, and water bath ultrasonication specifically include: The jellyfish fatty acid lipid solution was transferred to a round-bottom flask, and then the flask containing the jellyfish fatty acid lipid solution was placed in a rotary evaporator and evaporated at 55-65℃ and 0.02-0.06MPa to form a jellyfish fatty acid lipid film. Then, PBS buffer containing 1% EDTA was added to the jellyfish fatty acid lipid film to obtain a reconstituted solution. Finally, the round-bottom flask containing the reconstituted solution was sonicated at 180-220W and 35-45kHz for 8-12 minutes to obtain the jellyfish fatty acid lipid colostrum.

[0013] According to a preferred embodiment of the present invention, in step (4), the high-pressure homogenization and freeze-drying specifically comprises: The jellyfish fatty acid lipid colostrum was homogenized 2-4 times using a high-pressure homogenizer at 450-550 bar to obtain a jellyfish fatty acid liposome suspension. Then, 5% trehalose was added to the jellyfish fatty acid liposome suspension, and the mixture was freeze-dried at -40--50℃ and a vacuum degree ≤10Pa for 45-50 hours to obtain jellyfish fatty acid liposomes.

[0014] A jellyfish fatty acid liposome was prepared according to the above method.

[0015] The above-mentioned jellyfish fatty acid liposomes are used in the preparation of dressings and medical aesthetic drugs.

[0016] A liquid dressing containing jellyfish fatty acid liposomes comprises the following raw materials in parts by weight: Jellyfish fatty acid liposomes 0.5~1.5 parts, sodium hyaluronate 4.5~5.5 parts, glycerol 4.5~5.5 parts, jellyfish polypeptides 0.05~0.15 parts, vitamin E 0.15~0.25 parts, phenoxyethanol 0.05~0.15 parts, and ultrapure water to make up to 100 parts.

[0017] The preparation method of the above-mentioned liquid dressing containing jellyfish fatty acid liposomes includes: adding jellyfish fatty acid liposomes to ultrapure water according to the specified ratio, stirring until completely dissolved, continuing to add sodium hyaluronate, glycerin, jellyfish polypeptide, vitamin E and phenoxyethanol, adjusting the pH to 5.5~6.5, stirring and mixing evenly, filtering with a 0.22μm microporous membrane, sterilizing by cobalt-60 γ-ray irradiation with a dose of 20~30kGy, dispensing into sterile spray bottles or dropper bottles, and storing in the dark to obtain the liquid dressing containing jellyfish fatty acid liposomes.

[0018] The technical features of this invention are as follows: The jellyfish fatty acids extracted and used in this invention contain nearly 30 rare medium-chain fatty acids (C8-C12), such as 10-hydroxy-2-decenoic acid (HDA) and nonanoic acid. Their antibacterial, anti-inflammatory, and antioxidant activities are significantly better than those of traditional plant fatty acids. The inhibition rate against Staphylococcus aureus is over 90% (MIC=10μg / mL). The ability to scavenge DPPH free radicals is 2.5 times that of vitamin C. It reduces UV-induced oxidative stress and can also promote the secretion of type I collagen by fibroblasts by activating the TGF-β1 / Smad signaling pathway, thereby improving skin elasticity.

[0019] The jellyfish fatty acid liposomes prepared in this invention have a bilayer membrane structure highly similar to skin cell membranes. They can encapsulate both water-soluble (e.g., hyaluronic acid, polypeptides) and fat-soluble (e.g., vitamin E, squalane) components, protecting the active ingredients from the effects of light, heat, and pH, thus extending shelf life (reducing the degradation rate of active ingredients by 60% at room temperature) and achieving layered release. Furthermore, the particle size (100-300 nm) of these jellyfish fatty acid liposomes matches the intercellular space of the skin's stratum corneum, increasing the penetration rate by 3 times.

[0020] The beneficial effects of this invention are as follows: 1. This invention innovatively selects the most active fatty acids from jellyfish as functional substances to prepare jellyfish fatty acid liposomes and liquid dressings. These jellyfish fatty acids are rich in nearly thirty rare medium-chain fatty acids, possessing various biological activities such as antibacterial and anti-inflammatory properties, antioxidant activity, collagen production promotion, and free radical scavenging. They exhibit a 92% inhibition rate against Staphylococcus aureus and an 88% free radical scavenging rate. Compared to existing technologies, jellyfish fatty acids have extremely high application value and broad application prospects in skincare, healthcare, and other fields, effectively meeting market demand for products with multiple functions.

[0021] 2. This invention utilizes liposomes as a carrier to deliver jellyfish fatty acids, significantly improving their stability. Under conditions of 25℃ / 6 months, the degradation rate of active ingredients is less than 10%, greatly reducing activity loss during preparation and storage. Simultaneously, the liposome carrier significantly enhances the transdermal absorption rate of jellyfish fatty acids (90%), allowing the active ingredients to act more efficiently on the target site, enhancing the product's actual effectiveness and providing strong assurance for its efficacy. This demonstrates a clear technological advantage.

[0022] 3. This invention uses jellyfish fatty acid liposomes as its core and employs a strategy of incorporating natural additives, including vitamin E, glycerin, and hyaluronic acid, to form a liquid dressing. These natural additives not only help extend the product's shelf life and provide an additional protective barrier for the skin, but also significantly increase collagen synthesis, further enhancing the moisturizing and repairing functions of the liquid dressing.

[0023] 4. The method for preparing jellyfish fatty acid liposomes provided by this invention has a short process flow and simple operation, which is conducive to mass production and meeting the needs of a large-scale market. At the same time, the organic reagents used in the production process can be recycled and reused, and the remaining jellyfish components can be used as raw materials or nutritional supplements for other skin care products, effectively improving resource utilization and better controlling production costs, demonstrating excellent performance in both environmental protection and economic benefits. Detailed Implementation

[0024] The following embodiments of the present invention are merely illustrative of specific implementation schemes for implementing the present invention. These schemes should not be construed as limiting the present invention. Any changes made without departing from the principles and essence of the present invention shall fall within the protection scope of the present invention.

[0025] Unless otherwise specified, the experimental techniques and methods used in this embodiment are conventional. Unless otherwise specified, all materials and reagents used in this embodiment can be obtained through legitimate commercial channels. Room temperature refers to 20~30℃.

[0026] In the examples, the jellyfish polypeptide is available from Xi'an Ruixi Biotechnology Co., Ltd.

[0027] Chloroform and methanol were mixed evenly at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solvent.

[0028] Example 1 A method for extracting fatty acids from jellyfish, the specific steps of which are as follows: S1: Moon jellyfish is selected as the extraction raw material. The internal organs and other impurities of the moon jellyfish are removed. The internal organs may contain some components that are not conducive to extraction. Removing impurities can ensure the purity of the extract. Then, the cleaned jellyfish is stored at -40℃ for 24 hours. Low temperature freezing can effectively preserve the active substances in the jellyfish and prevent them from being destroyed during the pulverization process. Then, it is pulverized to 200 mesh to obtain jellyfish powder. The fine powder helps the effective components to dissolve fully in the subsequent extraction process. S2: Add anhydrous ethanol to the jellyfish powder at a material-to-liquid ratio of 1:10, and extract ultrasonically at 55℃, 300W, and 40kHz for 2 hours. Anhydrous ethanol can effectively dissolve fatty acids and other components in the jellyfish. The cavitation effect of ultrasound can accelerate cell rupture, allowing the active ingredients to be released into the solvent more quickly, thus improving the extraction efficiency. Centrifuge the resulting mixed solution at 8000rpm for 15 minutes, and collect the supernatant. Centrifugation can separate the solid residue in the solution from the supernatant containing jellyfish fatty acids. Repeat the ultrasonic extraction and centrifugation three times, and combine the supernatants obtained from the three extractions to obtain the jellyfish extract. S3: The jellyfish extract was rotary evaporated at 55℃ and 0.08MPa until the anhydrous ethanol was completely evaporated to obtain crude jellyfish fatty acid extract; S4: Dissolve the crude jellyfish fatty acid extract in ultrapure water, and add n-hexane at a volume ratio of 1:3 to obtain the extraction system. Hexane and water are immiscible and will form a layered phenomenon. The partition coefficient of jellyfish fatty acid is different in different solvents. In this way, impurities can be initially separated from jellyfish fatty acid. Shake the extraction system at 200 rpm for 10 min, and then let it stand until the layers separate. Take the aqueous phase. The aqueous phase is rich in jellyfish fatty acid, while most of the impurities remain in the n-hexane phase. Repeat the shaking extraction and standing process 3 times in total. Combine the aqueous phases obtained from the 3 times to obtain the jellyfish fatty acid extract. S5, the jellyfish fatty acid extract was freeze-dried at -50℃ for 48h to obtain jellyfish fatty acids. Freeze-drying can remove water from the aqueous phase, and its purity can reach ≥95%.

[0029] Example 2 A method for preparing jellyfish fatty acid liposomes includes the following steps: (1) 20 mg of jellyfish fatty acid prepared in Example 1, 100 mg of soybean lecithin and 20 mg of cholesterol were added to 16 mL of chloroform-methanol mixed solvent to obtain jellyfish fatty acid lipid solution; Among them, jellyfish fatty acids, as the core functional component, endow liposomes with unique biological activity; soybean lecithin is the main substance constituting the liposome bilayer, providing the basic structural framework for liposomes; cholesterol can regulate the fluidity and stability of the liposome membrane. The three work together to determine the performance of liposomes; the solvent formed by mixing chloroform and methanol in a specific ratio has good solubility, which can fully dissolve jellyfish fatty acids, soybean lecithin and cholesterol to form a homogeneous lipid solution, laying the foundation for the subsequent film formation. (2) Carefully pour the jellyfish fatty acid lipid solution into a round-bottom flask, place the round-bottom flask containing the jellyfish fatty acid lipid solution in a rotary evaporator, set the temperature to 60℃ and the pressure to 0.05MPa for rotary evaporation; during the rotation process, the solvent continuously evaporates, and the lipid molecules gradually form a uniform jellyfish fatty acid lipid film on the inner wall of the flask. 10 mL of phosphate buffer (PBS, pH=7.4, containing 0.1% EDTA) was added to the formed jellyfish fatty acid lipid film to obtain a reconstituted solution. Finally, the round-bottom flask containing the reconstituted solution was placed in a 60°C water bath and sonicated at 200W and 40kHz for 10 min to obtain the jellyfish fatty acid lipid colostrum.

[0030] (3) The jellyfish fatty acid lipid colostrum was homogenized three times at 500 bar using a high-pressure homogenizer to obtain a jellyfish fatty acid liposome suspension; then 5% trehalose was added to the jellyfish fatty acid liposome suspension and freeze-dried at -45℃ and vacuum degree ≤10Pa for 48h to obtain jellyfish fatty acid liposomes.

[0031] The strong shearing and impact forces break down and refine the liposomes in colostrum, breaking larger liposome particles into smaller ones. Through three cycles of processing, the liposome particle size becomes more uniform, ultimately yielding a liposome suspension with a particle size of 150±20 nm. This uniform particle size helps improve the stability and bioavailability of the liposomes, allowing them to function better in vivo.

[0032] Example 3 A method for preparing jellyfish fatty acid liposomes includes the following steps: (1) 15 mg of jellyfish fatty acid prepared in Example 1, 100 mg of soybean lecithin and 25 mg of cholesterol were added to 14 mL of chloroform-methanol mixed solvent to obtain jellyfish fatty acid lipid solution; (2) Carefully pour the jellyfish fatty acid lipid solution into a round-bottom flask, place the round-bottom flask containing the jellyfish fatty acid lipid solution in a rotary evaporator, set the temperature to 45℃ and the pressure to 0.06MPa for rotary evaporation; during the rotation process, the solvent continuously evaporates, and the lipid molecules gradually form a uniform jellyfish fatty acid lipid film on the inner wall of the flask. 10 mL of phosphate buffer (PBS, pH=7.4, containing 0.1% EDTA) was added to the formed jellyfish fatty acid lipid film to obtain a reconstituted solution. Finally, the round-bottom flask containing the reconstituted solution was placed in a 60°C water bath and sonicated at 200W and 40kHz for 10 min to obtain the jellyfish fatty acid lipid colostrum.

[0033] (3) The jellyfish fatty acid lipid colostrum was homogenized three times at 480 bar using a high-pressure homogenizer to obtain a jellyfish fatty acid liposome suspension; then 5% trehalose was added to the jellyfish fatty acid liposome suspension and freeze-dried at -45℃ and vacuum degree ≤10Pa for 48h to obtain jellyfish fatty acid liposomes.

[0034] Example 4 A liquid dressing containing jellyfish fatty acid liposomes comprises the following raw materials in parts by weight: Example 2 prepared 1g of jellyfish fatty acid liposomes, 5g of sodium hyaluronate, 5g of glycerol, 0.1g of jellyfish polypeptide, 0.2g of vitamin E, 0.1g of phenoxyethanol, and ultrapure water to make up to 100g.

[0035] The preparation method of the above-mentioned liquid dressing containing jellyfish fatty acid liposomes includes: adding jellyfish fatty acid liposomes to ultrapure water according to the ratio, stirring until completely dissolved, continuing to add sodium hyaluronate, glycerin, jellyfish polypeptide, vitamin E and phenoxyethanol, adjusting the pH to 6, stirring and mixing evenly, filtering with a 0.22μm microporous membrane, sterilizing by cobalt-60 γ-ray irradiation with a dose of 25kGy, dispensing into sterile spray bottles or dropper bottles, and storing in the dark to obtain the liquid dressing containing jellyfish fatty acid liposomes.

[0036] Example 5 A liquid dressing containing jellyfish fatty acid liposomes comprises the following raw materials in parts by weight: Example 2 prepared 0.8g of jellyfish fatty acid liposomes, 4.5g of sodium hyaluronate, 5.5g of glycerol, 0.08g of jellyfish polypeptide, 0.22g of vitamin E, 0.1g of phenoxyethanol, and ultrapure water to make up to 100g.

[0037] The specific preparation method is the same as in Example 3.

[0038] Example 6 A liquid dressing containing jellyfish fatty acid liposomes comprises the following raw materials in parts by weight: Example 2 prepared 1.2g of jellyfish fatty acid liposomes, 5.5g of sodium hyaluronate, 4.5g of glycerol, 0.1g of jellyfish polypeptide, 0.22g of vitamin E, 0.08g of phenoxyethanol, and ultrapure water to make up to 100g.

[0039] The specific preparation method is the same as in Example 3.

[0040] Comparative Example 1 A conventional liquid dressing comprises the following raw materials in parts by weight: 1g of plant fatty acids (olive oil extract), 5g of sodium hyaluronate, 5g of glycerin, 0.1g of regular collagen, 0.2g of vitamin E, 0.1g of phenoxyethanol, and ultrapure water to make up to 100g.

[0041] Test case 1. Antibacterial rate (Staphylococcus aureus) determination Staphylococcus aureus was inoculated onto LB solid agar medium using the agar diffusion method. Equal volumes of the liquid dressings prepared in Example 4 and Comparative Example 1 were added to each medium, serving as the experimental groups. LB solid agar medium inoculated with Staphylococcus aureus but without any added liquid dressing served as the blank control group. After incubation at 37°C for 24 hours, the diameter of the inhibition zone was measured, and the antibacterial rate was calculated.

[0042] Antibacterial rate = (inhibition zone area of ​​experimental group - inhibition zone area of ​​blank control group) / inhibition zone area of ​​blank control group × 100%.

[0043] 2. Determination of free radical scavenging rate (DPPH) Take 5 mL of the liquid dressing prepared in Example 4 and Comparative Example 1 respectively, add 5 mL of 0.1 mmol / L DPPH ethanol solution, react in the dark for 30 min, and measure the absorbance value at 517 nm (A1); at the same time, measure the absorbance of the liquid dressing and ethanol mixture prepared in Example 4 and Comparative Example 1 (A2), and the absorbance of the DPPH ethanol solution and ultrapure water mixture (A0), and calculate the free radical scavenging rate.

[0044] Free radical scavenging rate = [1 - (A1 - A2) / A0] × 100%.

[0045] 3. Determination of collagen synthesis Human skin fibroblasts were cultured in vitro and divided into a blank group, Example 4 group, and Comparative Example 1 group. Equal amounts of water, liquid dressings prepared in Example 4 and Comparative Example 1 were added to each group for 48 hours. The content of type I collagen in the cell supernatant was detected by ELISA kit. The relative synthesis amount of each group was calculated with the blank group as the baseline (100%).

[0046] 4. Transdermal absorption rate determination Using a Franz diffusion cell, rat skin was fixed between the supply chamber and the receiving chamber. The liquid dressing prepared in Example 4 and Comparative Example 1 was added to the supply chamber, and PBS buffer was added to the receiving chamber. The mixture was stirred at a constant temperature of 32°C, and samples were taken at regular intervals. The content of active ingredients in the receiving chamber was determined by high performance liquid chromatography, and the transdermal absorption rate was calculated.

[0047] 5. Stability (25℃ / 6 months) test The liquid dressings prepared in Example 4 and Comparative Example 1 were stored in a constant temperature chamber at 25°C for 6 months. Samples were taken at 0 months, 3 months and 6 months to determine the content of active ingredients and calculate the degradation rate.

[0048] Degradation rate = (initial content - content after 6 months) / initial content × 100%.

[0049] The results of the antibacterial rate, free radical scavenging rate, collagen synthesis, transdermal absorption rate and stability of the above experiments are shown in Table 1 below.

[0050] Table 1 As shown in Table 1, the liquid dressing prepared in Example 4 showed an inhibition rate of 92% against Staphylococcus aureus, a free radical scavenging rate of 88%, a collagen synthesis rate of 185%, and a transdermal absorption rate of 90%. Under the conditions of 25℃ / 6 months, the degradation rate of active ingredients was less than 10%, all of which were significantly better than the traditional liquid dressing in Comparative Document 1.

[0051] The embodiments described above are merely preferred implementations of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing jellyfish fatty acid liposomes, characterized in that, The steps include the following: (1) Jellyfish fatty acids were obtained by crushing, extracting with organic solvents, purifying and freeze-drying the jellyfish. (2) Jellyfish fatty acids, soybean lecithin and cholesterol are added sequentially to a chloroform-methanol mixed solvent to obtain a jellyfish fatty acid lipid solution; (3) The jellyfish fatty acid lipid solution was prepared into a jellyfish fatty acid lipid film by thin film dispersion method, and then the jellyfish fatty acid lipid film was redissolved and subjected to water bath sonication to obtain jellyfish fatty acid lipid colostrum; (4) Jellyfish fatty acid lipid colostrum was subjected to high pressure homogenization and freeze drying to obtain jellyfish fatty acid liposomes.

2. The preparation method according to claim 1, characterized in that, The jellyfish in question is either the moon jellyfish or the jellyfish moniliforme.

3. The preparation method according to claim 1, characterized in that, The specific steps of pulverization, extraction, purification, and freeze-drying are as follows: S1: Select fresh jellyfish as the extraction raw material, remove the jellyfish's internal organs and other impurities, then store the cleaned jellyfish at -35~-45℃ for 20~30h, and then pulverize it to 180~220 mesh to obtain jellyfish powder; S2: Add anhydrous ethanol to the jellyfish powder at a material-to-liquid ratio of 1:10, and extract ultrasonically at 55-65℃, 200-300W, and 40kHz for 1.5-2.5 hours. Centrifuge the resulting mixture at 7500-8500rpm for 12-18 minutes and collect the supernatant. Repeat the ultrasonic extraction and centrifugation 2-3 times, and combine all the supernatants to obtain the jellyfish extract. S3: The jellyfish extract was rotary evaporated at 45~55℃ and 0.05~0.1MPa until the anhydrous ethanol was completely evaporated to obtain crude jellyfish fatty acid extract; S4: Dissolve the crude fatty acid extract of jellyfish in ultrapure water, add n-hexane at a volume ratio of 1:3 to obtain the extraction system; shake the extraction system at 150~200 rpm for 10~15 min, then let it stand until the layers are separated, and take the aqueous phase; repeat the shaking extraction and standing for a total of 2~3 times, combine all the aqueous phases to obtain the jellyfish fatty acid extract; S5. Jellyfish fatty acid extract was freeze-dried at -45~-55℃ for 45~50h to obtain jellyfish fatty acid.

4. The preparation method according to claim 1, characterized in that, In step (2), the mass-volume ratio of the jellyfish fatty acid, soybean lecithin, cholesterol, and chloroform-methanol mixed solvent is (15~25):(80~120):(15~25):(10~20), unit: mg / mg / mg / mL; The chloroform-methanol mixed solvent is prepared by mixing chloroform and methanol in a volume ratio of 2:

1.

5. The preparation method according to claim 1, characterized in that, In step (3), the thin film dispersion method, resolution, and water bath ultrasonication specifically refer to: The jellyfish fatty acid lipid solution was transferred to a round-bottom flask, and then the flask containing the jellyfish fatty acid lipid solution was placed in a rotary evaporator and evaporated at 55-65℃ and 0.02-0.06MPa to form a jellyfish fatty acid lipid film. Then, PBS buffer containing 1% EDTA was added to the jellyfish fatty acid lipid film to obtain a reconstituted solution. Finally, the round-bottom flask containing the reconstituted solution was sonicated at 180-220W and 35-45kHz for 8-12 minutes to obtain the jellyfish fatty acid lipid colostrum.

6. The preparation method according to claim 1, characterized in that, In step (4), the high-pressure homogenization and freeze-drying specifically involve: The jellyfish fatty acid lipid colostrum was homogenized 2-4 times using a high-pressure homogenizer at 450-550 bar to obtain a jellyfish fatty acid liposome suspension. Then, 5% trehalose was added to the jellyfish fatty acid liposome suspension, and the mixture was freeze-dried at -40--50℃ and a vacuum degree ≤10Pa for 45-50 hours to obtain jellyfish fatty acid liposomes.

7. A jellyfish fatty acid liposome, characterized in that, Prepared according to the method described in any one of claims 1 to 6.

8. The use of the jellyfish fatty acid liposomes according to claim 7 in the preparation of dressings and medical aesthetic drugs.

9. A liquid dressing containing jellyfish fatty acid liposomes, characterized in that, The raw materials include the following parts by weight: Jellyfish fatty acid liposomes 0.5~1.5 parts, sodium hyaluronate 4.5~5.5 parts, glycerol 4.5~5.5 parts, jellyfish polypeptides 0.05~0.15 parts, vitamin E 0.15~0.25 parts, phenoxyethanol 0.05~0.15 parts, and ultrapure water to make up to 100 parts.

10. The method for preparing the liquid dressing containing jellyfish fatty acid liposomes according to claim 9, characterized in that, include: According to the formula, jellyfish fatty acid liposomes are added to ultrapure water and stirred until completely dissolved. Sodium hyaluronate, glycerin, jellyfish polypeptides, vitamin E and phenoxyethanol are then added to adjust the pH to 5.5-6.

5. After stirring and mixing evenly, the mixture is filtered through a 0.22μm microporous membrane and sterilized by cobalt-60 gamma irradiation at a dose of 20-30kGy. The mixture is then dispensed into sterile spray bottles or dropper bottles and stored in the dark to obtain a liquid dressing containing jellyfish fatty acid liposomes.

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