Cell electrotransduction instrument with dustproof mechanism for gene editing

Abstract

The invention discloses a cell electroporation instrument with a dustproof mechanism for gene editing, the cell electroporation instrument comprises a cell electroporation instrument body and a cell test tube, the top of the cell electroporation instrument body is fixedly connected with a fixed seat, the top wall of the fixed seat is rotatably connected with a first protective sleeve, and the inner side wall of the first protective sleeve is symmetrically provided with first chutes; the side wall of each first sliding groove is fixedly connected with a sliding rod, the side wall of each sliding rod is slidably connected with a first sliding block, the side walls of the two first sliding blocks are jointly and fixedly connected with a second protection sleeve, the inner side wall of the second protection sleeve is fixedly connected with a stabilizing block, and a through hole is formed in the top wall of the stabilizing block. By arranging the first protective sleeve, the second protective sleeve, a stabilizing block, an abutting block and a second spring component, dust is prevented from entering the cell test tube, the cell test tube is stably placed, and by arranging a gear, a first rack plate, a second rack plate, a second sliding block, a mounting block, a T-shaped rod and a clamping block component, the angle of a placing hole can be adjusted and locked.

Description

Technical Field

[0001] This invention relates to the field of electroporation technology, and more particularly to a cell electroporator for gene editing with a dustproof mechanism. Background Technology

[0002] Electroporation, also known as cell electroporation, is an important method for introducing exogenous macromolecules such as DNA, RNA, siRNA, and proteins, as well as some small molecules, into the cell membrane. It has wide applications in gene editing, embryo modification, cell mechanism research, protein production, and cell therapy.

[0003] When users need to introduce DNA into cells, they typically use an electroporator for gene editing. However, existing electroporators for gene editing still have some shortcomings in use. For example, after injecting a liquid sample into the cell tube until the liquid surface bulges, the overflowing liquid comes into contact with the external ring groove. Existing devices usually do not have a dustproof mechanism, allowing external dust to enter the electroporation tube inside the ring groove, which can easily contaminate the cells during electroporation. Furthermore, when using cell tubes of different sizes, if the cell tube is smaller than the inner cavity of the ring groove, it will form a cavity with the inner cavity of the ring groove, causing the cell tube to be unstable. Although some devices have a dustproof structure, their placement port is usually set in a fixed position, requiring the user to constantly move around to adjust the position, which is inconvenient. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a cell electroporator for gene editing with a dustproof mechanism.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A cell electroporator for gene editing with a dustproof mechanism includes a cell electroporator body and cell tubes. A fixed base is fixedly connected to the top of the cell electroporator body. A first protective sleeve is rotatably connected to the top wall of the fixed base. The inner sidewall of the first protective sleeve has symmetrically formed first grooves. A sliding rod is fixedly connected to the sidewall of each first groove. A first slider is slidably connected to the sidewall of each sliding rod. A second protective sleeve is fixedly connected to the sidewalls of two first sliders. A cover plate is fixedly connected to one end of the second protective sleeve. A stabilizing block is fixedly connected to the inner sidewall of the second protective sleeve. A through hole is formed on the top wall of the stabilizing block. Multiple grooves are formed at equal intervals along the circumference on the sidewall of the through hole. A stop block is slidably connected inside each groove. A second spring is fixedly connected to the sidewall of each stop block. The other end of each second spring is fixedly connected to the sidewall of the groove it belongs to.

[0006] Preferably, each of the abutments is a wedge-shaped structure, each of the abutments is in contact with the side wall of the cell tube, each of the groove side walls has a strip-shaped hole, each of the strip-shaped holes has a connecting rod slidably connected inside, one end of each connecting rod is fixedly connected to the side wall of the corresponding abutment, and the other end of each connecting rod is fixedly connected to a toggle plate, each toggle plate slides against the top wall of the stabilizing block.

[0007] Preferably, a fixing frame is fixedly connected to the top of the first protective sleeve. The fixing frame has a U-shaped structure. The second protective sleeve is slidably connected to the bottom wall of the fixing frame. Threaded holes are symmetrically opened on the side wall of the fixing frame. Each threaded hole is threaded with a limit bolt. Limit grooves are symmetrically opened on the side wall of the cover plate.

[0008] Preferably, the second protective sleeve has a placement hole on its side wall, the first protective sleeve has a transparent plate fixedly connected through it, and the first protective sleeve has a gear fixedly connected to its side wall.

[0009] Preferably, a mounting block is fixedly connected to the side wall of the cell electroporation instrument body, a second sliding groove is opened on the side wall of the mounting block, a second slider is slidably connected inside the second sliding groove, a first rack plate is fixedly connected to the side wall of the second slider, the first rack plate is meshed with a gear, and a handle is fixedly connected to the side wall of the second slider.

[0010] Preferably, a second rack plate is fixedly connected to the side wall of the second slider, a T-shaped rod is slidably connected through the side wall of the second slide groove, a clamping plate is fixedly connected to one end of the T-shaped rod, and a third spring is sleeved on the side wall of the T-shaped rod.

[0011] Preferably, one end of the third spring is fixed to the top wall of the mounting block, and the other end of the third spring is fixedly connected to one T-shaped end of the T-shaped rod.

[0012] Preferably, the top wall of the fixed seat is provided with an annular groove, and each slide rod side wall is fitted with a first spring, one end of each first spring being fixedly connected to the side wall of the first slide groove.

[0013] The present invention has the following beneficial effects: 1. By setting up a placement hole, a first protective sleeve, a second protective sleeve, a stabilizing block, a stop block, a second spring, a groove, a strip hole, a connecting rod, and a toggle plate, the second protective sleeve is pulled upwards to cause the two first sliders to slide upwards along the sliding rod, so that the placement hole opened on the side wall of the second protective sleeve is fully exposed. At this time, the operator puts the cell tube into the placement hole, so that the cell tube passes through the through hole opened on the top wall of the stabilizing block. During this process, the cell tube slides against the side wall of the stop block and squeezes the stop block to slide into the groove to compress the second spring. Under the elastic force of the second spring, the stop block is tightly pressed against the side wall of the cell tube, so as to clamp and fix the cell tube, making its placement more stable. Furthermore, the first and second protective sleeves can isolate external dust and prevent it from entering the electroshock tube inside the annular groove, thus preventing contamination during cell electroshock. 2. By setting up a first spring, a fixing frame, a limiting groove, a limiting bolt, and a threaded hole component, the cover plate is pressed down to drive the second protective sleeve to slide down, allowing the cell tube to extend into the annular groove. During this process, the first slider slides down along the slide rod and compresses the first spring, buffering the pressure transmitted by the cover plate to prevent excessive pressure from damaging the cell tube. The cover plate descends along with the second protective sleeve. When the limiting grooves symmetrically opened on the side wall of the cover plate are at the same horizontal position as the limiting bolt, the limiting bolt is rotated to rotate into the limiting groove, thereby limiting the second protective sleeve and making the cell tube more stable during operation. 3. By setting up gears, a first rack plate, a second rack plate, a second slider, a second slide groove, a mounting block, a handle, a T-shaped rod, and a locking block, the user can pull the T-shaped rod to stretch the third spring, causing the locking plate to move upward and release the lock on the second rack plate. At this time, pulling the handle can drive the second slider to slide along the second slide groove, and drive the first rack plate to move synchronously. Through the meshing action of the first rack plate and the gear, the first protective sleeve can be rotated through a certain angle to adjust it to a direction that is easy to operate without having to walk to adjust the position, making it convenient to use. After adjustment, releasing the T-shaped rod will cause the T-shaped rod and the locking plate to reset under the elastic force of the third spring, limiting the second rack plate again. At this time, the second slider cannot be pulled by the handle, thus locking the first protective sleeve after adjusting the angle. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the external structure of a gene editing cell electroporator with a dustproof mechanism proposed in this invention; Figure 2 This is a schematic diagram of the internal structure of the first and second protective sleeves in a gene editing cell electroporator with a dustproof mechanism proposed in this invention; Figure 3 for Figure 2Enlarged schematic diagram of part A of the structure; Figure 4 This is a cross-sectional schematic diagram of the mounting block portion in a gene editing cell electroporator with a dustproof mechanism proposed in this invention; Figure 5 for Figure 2 An enlarged schematic diagram of the structure of part B in the middle section.

[0015] In the diagram: 1. Cell electroporation instrument body; 2. Fixing base; 3. Annular groove; 4. First protective sleeve; 5. Cover plate; 6. Cell tube; 7. First slide groove; 8. First slider; 9. Slide rod; 10. First spring; 11. Second protective sleeve; 12. Stabilizing block; 13. Through hole; 14. Abutment block; 15. Groove; 16. Second spring; 17. Connecting rod; 18. Actuating plate; 19. Strip hole; 20. Fixing frame; 21. Limiting groove; 22. Threaded hole; 23. Limiting bolt; 24. Placement hole; 25. Transparent plate; 26. Gear; 27. Mounting block; 28. Second slider; 29. ​​First rack plate; 30. Second rack plate; 31. Handle; 32. Second slide groove; 33. T-shaped rod; 34. Third spring; 35. Clamping plate. Detailed Implementation

[0016] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0017] Reference Figures 1-5 A cell electroporator for gene editing with a dustproof mechanism includes a cell electroporator body 1 and cell tubes 6. A fixed base 2 is fixedly connected to the top of the cell electroporator body 1. A first protective sleeve 4 is rotatably connected to the top wall of the fixed base 2. The inner side wall of the first protective sleeve 4 is symmetrically provided with first grooves 7. Each side wall of the first groove 7 is fixedly connected with a slide rod 9. Each side wall of the slide rod 9 is slidably connected with a first slider 8. The side walls of the two first sliders 8 are jointly fixedly connected with a second protective sleeve 11. One end of the second protective sleeve 11 is fixedly connected with a cover plate 5. The inner side wall of the second protective sleeve 11 is fixedly connected with a stabilizing block 12. The top wall of the stabilizing block 12 is provided with a through hole 13. The side wall of the through hole 13 is provided with multiple grooves 15 equidistantly arranged in the circumferential direction. Each groove 15 is slidably connected with a stop block 14. The side wall of each stop block 14 is fixedly connected with a second spring 16. The other end of each second spring 16 is fixedly connected to the side wall of the groove 15.

[0018] Each abutment 14 is a wedge-shaped structure, and each abutment 14 abuts against the side wall of the cell tube 6, thereby ensuring the cell tube 6 is placed stably. Each groove 15 has a strip-shaped hole 19 on its side wall, and a connecting rod 17 is slidably connected inside each strip-shaped hole 19. One end of each connecting rod 17 is fixedly connected to the side wall of the corresponding abutment 14, and the other end of each connecting rod 17 is fixedly connected to a toggle plate 18. Each toggle plate 18 slides against the top wall of the stabilizing block 12. By using the toggle plate 18, the abutment 14 can be driven to slide into the groove 15, thereby disengaging from the side wall of the cell tube 6 and making it easier to remove.

[0019] The top of the first protective sleeve 4 is fixedly connected to a fixing frame 20, which has a U-shaped structure. The second protective sleeve 11 is slidably connected to the bottom wall of the fixing frame 20. The side wall of the fixing frame 20 is symmetrically provided with threaded holes 22, and each threaded hole 22 is threaded with a limit bolt 23. The side wall of the cover plate 5 is symmetrically provided with limit grooves 21. The limit bolts 23 and the limit grooves 21 enable the second protective sleeve 11 to be fixed and isolated from external dust when the instrument is working, and further make the cell tube 6 stable.

[0020] The second protective sleeve 11 has a placement hole 24 on its side wall, and a transparent plate 25 is fixedly connected through the side wall of the first protective sleeve 4 to facilitate the user to observe the internal situation. A gear 26 is fixedly connected to the side wall of the first protective sleeve 4.

[0021] A mounting block 27 is fixedly connected to the side wall of the cell electroporation instrument body 1. A second sliding groove 32 is opened on the side wall of the mounting block 27. A second slider 28 is slidably connected inside the second sliding groove 32. A first rack plate 29 is fixedly connected to the side wall of the second slider 28. The first rack plate 29 is meshed with a gear 26. A handle 31 is fixedly connected to the side wall of the second slider 28. Through the meshing of the first rack plate 29 and the gear 26, the first protective sleeve 4 can be rotated, thereby adjusting the angle of the placement hole 24 so that it faces the user for easy operation.

[0022] The second slider 28 is fixedly connected to the side wall of the second rack plate 30, and the second slide groove 32 is slidably connected to the side wall of the second slide groove 32. One end of the T-rod 33 is fixedly connected to the locking plate 35, and the side wall of the T-rod 33 is fitted with a third spring 34. The locking plate 35 and the second rack plate 30 can lock the second slide groove 30 after adjusting the angle of the placement hole 24.

[0023] One end of the third spring 34 is fixed to the top wall of the mounting block 27, and the other end of the third spring 34 is fixedly connected to the T-shaped end of the T-shaped rod 33. The elastic force of the third spring 34 can drive the clamping plate 35 and the T-shaped rod 33 to reset.

[0024] The top wall of the fixed seat 2 is provided with an annular groove 3, and each slide rod 9 is fitted with a first spring 10 on its side wall. One end of each first spring 10 is fixedly connected to the side wall of the first slide groove 7. The pressure transmitted by the cover plate 5 is buffered by the first spring 10 to avoid excessive pressure causing the cell tube 6 to be squeezed and damaged.

[0025] In this invention, by pulling the cover plate 5 upward, the second protective sleeve 11 causes the two first sliders 8 to slide upward along the slide rod 9, eventually exposing the placement hole 24 on the side wall of the second protective sleeve 11. At this time, the operator puts the cell tube 6 into the placement hole 24. Since the cell tube 6 is smaller than the inner cavity of the annular groove 3, it will form a cavity with the inner cavity of the annular groove 3, causing the cell tube 6 to be unstable. Therefore, the cell tube 6 needs to pass through the through hole 13 on the top wall of the stabilizing block 12. During this process, the cell tube 6 slides against the side wall of the abutment block 14 and squeezes the abutment block 14 to slide into the groove 15 to compress the second spring 16. Under the elastic force of the second spring 16, the abutment block 14 is tightly against the side wall of the cell tube 6, thereby clamping and fixing the cell tube 6, making its placement more stable. Furthermore, the first protective sleeve 4 and the second protective sleeve 11 can isolate external dust and prevent it from entering the electroshock tube inside the annular groove 3, causing contamination during cell electroshock.

[0026] After fixing, press down on the cover plate 5 to make it slide down on the second protective sleeve 11, so that the cell tube 6 extends into the annular groove 3. During this process, the first slider 8 slides down along the slide rod 9 and compresses the first spring 10 to buffer the pressure transmitted by the cover plate 5, so as to avoid excessive pressure that could cause the cell tube 6 to be squeezed and damaged. The cover plate 5 descends as the second protective sleeve 11 descends. When the limiting groove 21 symmetrically opened on the side wall of the cover plate 5 and the limiting bolt 23 are at the same horizontal position, the limiting bolt 23 is rotated to rotate into the limiting groove 21, thereby limiting the second protective sleeve 11 and making the cell tube 6 more stable during operation.

[0027] The user can pull the T-shaped rod 33 to stretch the third spring 34, causing the locking plate 35 to move upward and release its lock on the second rack plate 30. At this time, by pulling the handle 31, the second slider 28 can slide along the second slide groove 32, and the first rack plate 29 can move synchronously. Through the meshing action of the first rack plate 29 and the gear 26, the first protective sleeve 4 can be rotated by a certain angle and adjusted to a direction that is easy to operate without having to move to adjust the position, making it more convenient to use. After the adjustment is completed, the T-shaped rod 33 is released, and under the elastic force of the third spring 34, the T-shaped rod 33 and the locking plate 35 are reset, and the second rack plate 30 is limited again. At this time, the second slider 28 cannot be pulled by the handle 31, so that the first protective sleeve 4 can be locked after the angle is adjusted.

[0028] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A cell electroporator for gene editing with a dustproof mechanism, comprising a cell electroporator body (1) and cell tubes (6), characterized in that, The cell electroporation instrument body (1) is fixedly connected to a fixed base (2) at the top. The top wall of the fixed base (2) is rotatably connected to a first protective sleeve (4). The inner side wall of the first protective sleeve (4) is symmetrically provided with first sliding grooves (7). Each side wall of the first sliding groove (7) is fixedly connected to a sliding rod (9). Each side wall of the sliding rod (9) is slidably connected to a first slider (8). The side walls of the two first sliders (8) are jointly fixedly connected to a second protective sleeve (11). One end of the second protective sleeve (11) is fixedly connected to a cover plate (5). The inner side wall of the second protective sleeve (11) is fixedly connected to a stabilizing block (12). The top wall of the stabilizing block (12) is provided with a through hole (13). The side wall of the through hole (13) is provided with multiple grooves (15) equidistantly arranged in the circumferential direction. Each groove (15) is slidably connected to a stop block (14). Each side wall of the stop block (14) is fixedly connected to a second spring (16). The other end of each second spring (16) is fixedly connected to the side wall of the groove (15).

2. The cell electroporator for gene editing with a dustproof mechanism according to claim 1, characterized in that, Each of the abutments (14) is a wedge-shaped structure, and each of the abutments (14) abuts against the side wall of the cell tube (6). Each of the grooves (15) has a strip hole (19) on its side wall. Each strip hole (19) is slidably connected to a connecting rod (17). One end of each connecting rod (17) is fixedly connected to the side wall of the corresponding abutment (14), and the other end of each connecting rod (17) is fixedly connected to a toggle plate (18). Each toggle plate (18) slides against the top wall of the stabilizing block (12).

3. The cell electroporator for gene editing with a dustproof mechanism according to claim 1, characterized in that, The first protective sleeve (4) is fixedly connected to a fixing frame (20) at its top end. The fixing frame (20) is a U-shaped structure. The second protective sleeve (11) is slidably connected to the bottom wall of the fixing frame (20). The fixing frame (20) has symmetrically opened threaded holes (22) on its side wall. Each threaded hole (22) is threaded with a limit bolt (23). The cover plate (5) has symmetrically opened limit grooves (21) on its side wall.

4. The cell electroporator for gene editing with a dustproof mechanism according to claim 1, characterized in that, The second protective sleeve (11) has a placement hole (24) on its side wall, and a transparent plate (25) is fixedly connected through the side wall of the first protective sleeve (4). A gear (26) is fixedly connected to the side wall of the first protective sleeve (4).

5. A cell electroporator for gene editing with a dustproof mechanism according to claim 4, characterized in that, The cell electroporation instrument body (1) has a mounting block (27) fixedly connected to its side wall. The mounting block (27) has a second sliding groove (32) on its side wall. A second slider (28) is slidably connected inside the second sliding groove (32). A first rack plate (29) is fixedly connected to the side wall of the second slider (28). The first rack plate (29) is meshed with a gear (26). A handle (31) is fixedly connected to the side wall of the second slider (28).

6. A cell electroporator for gene editing with a dustproof mechanism according to claim 5, characterized in that, The second slider (28) is fixedly connected to the side wall of the second rack plate (30), and the second slide groove (32) is slidably connected to the side wall of the T-shaped rod (33). One end of the T-shaped rod (33) is fixedly connected to the clamping plate (35), and the side wall of the T-shaped rod (33) is fitted with a third spring (34).

7. A cell electroporator for gene editing with a dustproof mechanism according to claim 6, characterized in that, One end of the third spring (34) is fixed to the top wall of the mounting block (27), and the other end of the third spring (34) is fixedly connected to the T-shaped end of the T-shaped rod (33).

8. A cell electroporator for gene editing with a dustproof mechanism according to claim 1, characterized in that, The top wall of the fixed seat (2) is provided with an annular groove (3), and each slide rod (9) is fitted with a first spring (10) on its side wall. One end of each first spring (10) is fixedly connected to the side wall of the first slide groove (7).

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