Fluorescent algae analysis system and method, electronic equipment and storage medium

Abstract

The invention discloses a fluorescent algae analysis system and method, electronic equipment and a storage medium. The fluorescent algae analysis system comprises a light source driving circuit, a turbidity light source, a plurality of fluorescent light sources, a reference detector, a fluorescence detector, a turbidity detector and a data calculation module, the light source driving circuit is respectively connected with the turbidity light source and each fluorescent light source and is used for driving the turbidity light source and each fluorescent light source to work; the reference detector is used for detecting light intensity data of the turbidity light source or setting the fluorescence light source; the turbidity detector is used for detecting the turbidity of a water body to be detected; the fluorescence detector is used for detecting data of excited fluorescence with different intensities; the data calculation module is used for calculating the turbidity data and the fluorescence data to obtain algae analysis data. According to the fluorescent algae analysis system and method, the electronic equipment and the storage medium provided by the invention, the data stability and accuracy can be improved, and the influence of turbidity on a measurement result is eliminated.

Description

Technical Field

[0001] This invention belongs to the field of water substance analysis technology, and relates to an analysis system, and more particularly to a fluorescent algae analysis system, method, electronic device and storage medium. Background Technology

[0002] Chlorophyll a is one of the most commonly used indicators reflecting the biomass of phytoplankton in water bodies and is also an important parameter of eutrophication. Currently used chlorophyll analyzers have the following defects: (1) Using a single wavelength light source to excite fluorescence can only obtain the total chlorophyll a data of all algae, and cannot distinguish the biomass of different algal communities. Moreover, because the fluorescence effect produced by different algal communities to different wavelength light sources varies, the fluorescence is uniformly converted to chlorophyll a during calculation, which can only obtain approximate data of chlorophyll a content in the water body, and the error is large compared with the actual data of each algal community. (2) Natural water bodies have different degrees of turbidity, which interferes with the detection of chlorophyll a. A single light source cannot eliminate the influence of turbidity, further amplifying the error. (3) The fluorescence effect of chlorophyll a is greatly affected by temperature and requires temperature compensation.

[0003] In view of this, there is an urgent need to design a new method for analyzing fluorescent algae in order to overcome at least some of the aforementioned shortcomings of existing analytical methods. Summary of the Invention

[0004] This invention provides a fluorescent algae analysis system, method, electronic device, and storage medium, which can improve data stability and accuracy and eliminate the influence of turbidity on measurement results.

[0005] To solve the above-mentioned technical problems, according to one aspect of the present invention, the following technical solution is adopted:

[0006] A fluorescent algae analysis system, comprising: a light source driving circuit, a turbidity light source, several fluorescent light sources, a reference detector, a fluorescence detector, a turbidity detector, and a data calculation module;

[0007] The light source driving circuit is connected to the turbidity light source and each fluorescent light source respectively, and is used to drive the operation of the turbidity light source and each fluorescent light source.

[0008] The reference detector is used to detect the light intensity data of the turbidity light source or the set fluorescent light source, and send the light intensity data to the light source driving circuit. The light source driving circuit adjusts the current supplied to the turbidity light source or the set fluorescent light source so that the turbidity light source or the set fluorescent light source reaches a stable state.

[0009] When the turbidity light source reaches a stable state, the light emitted by the turbidity light source penetrates the water body to be tested and reaches the turbidity detector, and the turbidity detector detects the turbidity of the water body to be tested.

[0010] When the fluorescent light source reaches a stable state, the light emitted by the fluorescent light source penetrates the water body to be tested, exciting the fluorescence of different algae and / or chlorophyll; the fluorescence detector is used to detect the fluorescence data of different intensities.

[0011] The data calculation module is used to obtain algae analysis data by calculating turbidity data and various fluorescence data.

[0012] In one embodiment of the present invention, the data calculation module is used to perform turbidity calculation and algae calculation;

[0013] The turbidity calculation process includes: First, calibration is performed before leaving the factory, using turbidity samples of a set standard concentration for measurement (e.g., using several standard concentrations of turbidity samples such as 1 NTU and 100 NTU for measurement), to obtain the relationship between the light intensity measured by the turbidity detector and the corresponding concentration, turbidity = K * turbidity detector light intensity + B; In actual measurement, the turbidity data can be obtained by substituting the detector data;

[0014] The influence coefficient of turbidity on the excitation light source is K = X * turbidity + B; the influence coefficient of turbidity on the fluorescence effect is k = x * turbidity + b;

[0015] Among them, the four coefficients X, B, x, and b are measured in the laboratory and are constants. In actual measurement, K and k can be calculated by obtaining the turbidity value.

[0016] The algae calculation process includes: different algae are numbered 1, 2, 3, ..., N, corresponding to different excitation light source numbers a, b, c, ..., n. Taking the first light source a as an example, the relationship between fluorescence and each algae is measured as Ia*K=k*(C1*I 1+C2*I2+C3*I3+...+CN*IN);

[0017] Where K and k are compensation coefficients obtained from turbidity; C is the content of a certain type of algae; I is the fluorescence effect coefficient of a certain type of algae, which is a constant; Ia is the fluorescence produced by light source a for all algae excited by light source a, that is, the fluorescence intensity measured by the fluorescence detector;

[0018] By sequentially measuring the light sources b, c, d, ..., n, a polynomial combination is obtained. Solving the equation, the content of each algae, C1, C2, C3, ..., CN, is calculated.

[0019] As one embodiment of the present invention, the fluorescent algae analysis system further includes optical devices, the optical devices including a first lens, a second lens, a first semi-transparent mirror, and a second semi-transparent mirror;

[0020] The turbidity light source transmits part of the light to the reference detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror. The first lens is provided between the reference detector and the second semi-transparent and semi-reflective mirror. The turbidity light source transmits part of the light through the water sample to be tested to the turbidity detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror.

[0021] The fluorescent light source transmits part of the light to the reference detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror; the fluorescent light source transmits part of the light through the water body to be tested to the fluorescent detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror, and the second lens is provided between the fluorescent detector and the water body to be tested.

[0022] In one embodiment of the present invention, the data calculation module is used to calculate the data of cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a by calculating the turbidity data and various fluorescence data.

[0023] As one embodiment of the present invention, the light source driving circuit includes a first operational amplifier U1A, a first operational amplifier U1B, a first MOSFET Q1, a second MOSFET Q2, a third MOSFET Q3, a first diode D1, a second capacitor C2, a first resistor R1, a second resistor R2, a fourth resistor R4, a fifth resistor R5, and a seventh resistor R7.

[0024] The first terminal of the fifth resistor R5 receives the first control signal, and the second terminal of the fifth resistor R5 is connected to the first terminal of the second capacitor C2 and the non-inverting input terminal of the first operational amplifier U1A, respectively. The second terminal of the second capacitor C2 is grounded.

[0025] The inverting input terminal of the first operational amplifier U1A is connected to the drain of the second MOS transistor Q2 and the first terminal of the seventh resistor R7, respectively, and the second terminal of the seventh resistor R7 is grounded.

[0026] The output terminal of the first operational amplifier U1A is connected to the gate of the second MOS transistor Q2; the source terminal of the second MOS transistor Q2 is connected to the non-inverting input terminal of the first operational amplifier U1B and the second terminal of the fourth resistor R4, respectively.

[0027] The first end of the fourth resistor R4 is connected to the power supply voltage and the first end of the first resistor R1, and the second end of the first resistor R1 is connected to the first end of the second resistor R2 and the inverting input terminal of the first B operational amplifier U1B.

[0028] The output terminal of the first operational amplifier U1B is connected to the gate of the first MOS transistor Q1, the source of the first MOS transistor Q1 is connected to the second terminal of the second resistor R2, and the drain of the first MOS transistor Q1 is connected to the source of the third MOS transistor Q3 and the positive terminal of the first diode D1, respectively.

[0029] The gate of the third MOS transistor Q3 is connected to the second control signal, and the drain of the third MOS transistor Q3 and the cathode of the first diode D1 are grounded respectively.

[0030] In one embodiment of the present invention, the reference detection circuit includes a second operational amplifier U2A, a reference detector D2, a first capacitor C1, a third capacitor C3, a third resistor R3, and a sixth resistor R6; the reference detector D2 includes a second diode;

[0031] The non-inverting input of the second operational amplifier U2A is grounded, and the inverting input of the second operational amplifier U2A is connected to the negative terminal of the reference detector D2, the first terminal of the first capacitor C1, and the first terminal of the third resistor R3, respectively; the positive terminal of the reference detector D2 is grounded.

[0032] The output terminal of the second operational amplifier U2A is connected to the second terminal of the first capacitor C1, the second terminal of the third resistor R3, and the first terminal of the sixth resistor R6, respectively; the second terminal of the sixth resistor R6 is connected to the first terminal of the third capacitor C3, and the second terminal of the third capacitor C3 is grounded.

[0033] In one embodiment of the present invention, the turbidity detection circuit includes a third detector D3, a current-to-voltage conversion circuit, a high-pass filter circuit, and a first differential amplifier circuit; the third detector D3 includes a third diode;

[0034] The current-to-voltage conversion circuit includes a third operational amplifier U3A, a fourth capacitor C4, and an eighth resistor R8. The non-inverting input of the third operational amplifier U3A is grounded, and the inverting input of the third operational amplifier U3A is connected to the negative terminal of the third detector D3, the first terminal of the fourth capacitor C4, and the first terminal of the eighth resistor R8. The positive terminal of the third detector D3 is grounded. The output of the third operational amplifier U3A is connected to the second terminal of the fourth capacitor C4 and the second terminal of the eighth resistor R8.

[0035] The high-pass filter circuit includes a fourth operational amplifier U4A, a fifth capacitor C5, a seventh capacitor C7, a ninth resistor R9, a first resistor R11, a third zero resistor R30, and a third first resistor R31.

[0036] The first end of the fifth capacitor C5 is connected to the output terminal of the third operational amplifier U3A. The second end of the fifth capacitor C5 is connected to the first end of the ninth resistor R9 and the first end of the seventh capacitor C7. The second end of the seventh capacitor C7 is connected to the second end of the first resistor R11 and the non-inverting input terminal of the fourth operational amplifier U4A. The first end of the first resistor R11 is grounded.

[0037] The inverting input terminal of the fourth operational amplifier U4A is connected to the second terminal of the third zero resistor R30 and the first terminal of the third first resistor R31, respectively; the first terminal of the third zero resistor R30 is grounded; the output terminal of the fourth operational amplifier U4A is connected to the second terminal of the ninth resistor R9 and the second terminal of the third first resistor R31, respectively.

[0038] The first differential amplifier circuit includes a fifth operational amplifier U5A, a sixth capacitor C6, an eighth capacitor C8, a first zero resistor R10, a first second resistor R12, a first third resistor R13, a first fourth resistor R14, and a first fifth resistor R15;

[0039] The inverting input terminal of the fifth operational amplifier U5A is connected to the second terminal of the first two resistors R12, the first terminal of the first zero resistor R10, and the first terminal of the sixth capacitor C6, respectively; the first terminal of the first two resistors R12 is connected to the output terminal of the fourth operational amplifier U4A.

[0040] The non-inverting input terminal of the fifth operational amplifier U5A is connected to the second terminal of the first four resistors R14 and the first terminal of the first five resistors R15, respectively; the first terminal of the first four resistors R14 is connected to the power supply voltage, and the second terminal of the first five resistors R15 is grounded.

[0041] The output terminal of the fifth operational amplifier U5A is connected to the second terminal of the first zero resistor R10, the second terminal of the sixth capacitor C6, and the first terminal of the first three resistors R13, respectively; the second terminal of the first three resistors R13 is connected to the first terminal of the eighth capacitor C8, and the second terminal of the eighth capacitor C8 is grounded.

[0042] In one embodiment of the present invention, the fluorescence detection circuit includes a fourth detector D4, a current-to-voltage conversion circuit, an inverting amplifier circuit, an inverting adder circuit, an integrating circuit, a temperature acquisition circuit, and a second differential amplifier circuit; the fourth detector D4 includes a fourth diode;

[0043] The current-to-voltage conversion circuit includes a seventh operational amplifier U7A, a first capacitor C11, and a first six resistor R16; the inverting input terminal of the seventh operational amplifier U7A is connected to the negative terminal of the fourth detector D4, the first terminal of the first capacitor C11, and the first terminal of the first six resistor R16, respectively, and the positive terminal of the fourth detector D4 is grounded.

[0044] The non-inverting input terminal of the seventh operational amplifier U7A is grounded, and the output terminal of the seventh operational amplifier U7A is connected to the second terminal of the first capacitor C11 and the second terminal of the first resistor R16, respectively.

[0045] The inverting amplifier circuit includes an eighth operational amplifier U8A, a first second capacitor C12, a first seventh resistor R17, and a first ninth resistor R19;

[0046] The inverting input terminal of the eighth operational amplifier U8A is connected to the second terminal of the first nine resistor R19, the first terminal of the first two capacitors C12, and the first terminal of the first seven resistor R17, respectively; the first terminal of the first nine resistor R19 is connected to the output terminal of the seventh operational amplifier U7A.

[0047] The non-inverting input terminal of the eighth operational amplifier U8A is grounded; the output terminal of the eighth operational amplifier U8A is connected to the second terminal of the first capacitor C12 and the second terminal of the first resistor R17, respectively.

[0048] The inverting adder circuit includes a third operational amplifier U3B, a first three-capacitor C13, a first eight-resistor R18, and a second zero-resistor R20.

[0049] The inverting input terminal of the third operational amplifier U3B is connected to the second terminal of the second zero resistor R20, the first terminal of the first three capacitors C13, and the first terminal of the first eight resistors R18, respectively; the first terminal of the second zero resistor R20 is connected to the output terminal of the eighth operational amplifier U8A.

[0050] The non-inverting input terminal of the third operational amplifier U3B is grounded, and the output terminal of the third operational amplifier U3B is connected to the second terminal of the first three capacitors C13 and the second terminal of the first eight resistors R18, respectively.

[0051] The integrating circuit includes a fourth operational amplifier U4B, a sixth switch U6A, a sixth switch U6B, a first fourth capacitor C14, a first sixth capacitor C16, a second first resistor R21, and a second second resistor R22.

[0052] The first end of the second resistor R21 is connected to the output terminal of the third operational amplifier U3B, and the second end of the second resistor R21 is connected to the first end of the sixth switch U6A.

[0053] The second terminal of the sixth A switch U6A is connected to the first terminal of the sixth B switch U6B, the first terminal of the first four capacitors C14, and the inverting input terminal of the fourth B operational amplifier U4B, respectively.

[0054] The non-inverting input terminal of the fourth operational amplifier U4B is grounded, and the output terminal of the fourth operational amplifier U4B is connected to the second terminal of the sixth switch U6B, the second terminal of the first four capacitors C14, and the first terminal of the second two resistors R22, respectively; the second terminal of the second two resistors R22 is connected to the first terminal of the first six capacitors C16, and the second terminal of the first six capacitors C16 is grounded.

[0055] The temperature acquisition circuit includes a ninth operational amplifier U9A, a second sixth resistor R26, and a second ninth temperature sensor R29.

[0056] The non-inverting input terminal of the ninth operational amplifier U9A is connected to the second terminal of the second six resistor R26 and the first terminal of the second nine temperature sensor R29, respectively; the first terminal of the second six resistor R26 is connected to the power supply voltage, and the second terminal of the second nine temperature sensor R29 is grounded; the inverting input terminal of the ninth operational amplifier U9A is connected to the output terminal of the ninth operational amplifier U9A.

[0057] The second differential amplifier circuit includes a fifth operational amplifier U5B, a first seventh capacitor C17, a second third resistor R23, a second fourth resistor R24, a second fifth resistor R25, a second seventh resistor R27, and a second eighth resistor R28.

[0058] The non-inverting input terminal of the fifth operational amplifier U5B is connected to the second terminal of the second fourth resistor R24 ​​and the first terminal of the second fifth resistor R25, respectively; the first terminal of the second fourth resistor R24 ​​is connected to the power supply voltage, and the second terminal of the second fifth resistor R25 is grounded.

[0059] The inverting input terminal of the fifth operational amplifier U5B is connected to the second terminal of the second seven resistor R27, the first terminal of the second eight resistor R28, and the first terminal of the first seven capacitor C17, respectively; the first terminal of the second seven resistor R27 is connected to the output terminal of the ninth operational amplifier U9A.

[0060] The output terminal of the fifth operational amplifier U5B is connected to the second terminal of the second eight resistor R28, the second terminal of the first seven capacitor C17, and the second terminal of the second three resistor R23, respectively; the first terminal of the second three resistor R23 is connected to the inverting input terminal of the third operational amplifier U3B.

[0061] According to another aspect of the present invention, the following technical solution is adopted: an analytical method for the above-mentioned fluorescent algae analysis system, the analytical method comprising:

[0062] The light source driving circuit drives the turbidity light source to work; the reference detector detects the light intensity data of the turbidity light source and sends the light intensity data to the light source driving circuit; the light source driving circuit adjusts the current supplied to the turbidity light source so that the turbidity light source or the set fluorescent light source reaches a stable state.

[0063] When the turbidity light source reaches a stable state, the light emitted by the turbidity light source penetrates the water body to be tested and reaches the turbidity detector, and the turbidity detector detects the turbidity of the water body to be tested.

[0064] The light source driving circuit sequentially drives the operation of each fluorescent light source; the reference detector detects the light intensity data of the set fluorescent light source and sends the light intensity data to the light source driving circuit, and the light source driving circuit adjusts the current supplied to the set fluorescent light source so that the turbidity light source or the set fluorescent light source reaches a stable state.

[0065] When the fluorescent light source reaches a stable state, the light emitted by the fluorescent light source penetrates the water body to be tested, exciting the fluorescence of different algae and / or chlorophyll; the fluorescence detector detects the fluorescence data of different intensities.

[0066] The data calculation module calculates algae analysis data by analyzing turbidity data and various fluorescence data.

[0067] According to another aspect of the present invention, the following technical solution is adopted: an electronic device, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the steps of the above method.

[0068] According to another aspect of the present invention, the following technical solution is adopted: a storage medium storing computer program instructions thereon, which, when executed by a processor, implement the steps of the above-described method.

[0069] The beneficial effects of this invention are as follows: The fluorescent algae analysis system, method, electronic device, and storage medium proposed in this invention can improve data stability and accuracy, and eliminate the influence of turbidity on measurement results. In one application scenario of this invention, the driving circuit drives eight different light sources respectively, modulates the driving current and scintillation frequency, and a dedicated acquisition circuit compensates for the stability of the light sources; the fluorescence acquisition circuit is specially designed with temperature compensation, which is directly completed by the hardware circuit without additional calculation, greatly improving data stability and accuracy; the added turbidity compensation function eliminates the influence of turbidity on the measurement results. Attached Figure Description

[0070] Figure 1 This is a schematic diagram of the structure of a fluorescent algae analysis system in one embodiment of the present invention.

[0071] Figure 2 This is a schematic diagram of the composition of a fluorescent algae analysis system in one embodiment of the present invention.

[0072] Figure 3 This is a circuit diagram of a light source driving circuit in one embodiment of the present invention.

[0073] Figure 4 This is a circuit diagram of a reference detection circuit in one embodiment of the present invention.

[0074] Figure 5 This is a circuit diagram of a turbidity detection circuit in one embodiment of the present invention.

[0075] Figure 6 This is a circuit diagram of a fluorescence detection circuit in one embodiment of the present invention.

[0076] Figure 7This is a flowchart of a fluorescent algae analysis method in one embodiment of the present invention.

[0077] Figure 8 This is a schematic diagram of the composition of an electronic device according to an embodiment of the present invention. Detailed Implementation

[0078] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0079] To further understand the present invention, preferred embodiments of the present invention are described below in conjunction with examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, and are not intended to limit the scope of the claims of the present invention.

[0080] The description in this section pertains to only a few typical embodiments, and the present invention is not limited to the scope of the embodiments described. Substitution of identical or similar prior art methods with some technical features in the embodiments is also within the scope of the description and protection of this invention.

[0081] The steps described in the various embodiments in the specification are for illustrative purposes only, and the implementation of this application is not limited by the order of the steps.

[0082] The term "connection" in the specification includes both direct and indirect connections, such as connections made through active devices, passive devices, or electrical conduction media; it may also include connections made by other active or passive devices that are known to those skilled in the art and can achieve the same or similar functional purpose, such as connections made through circuits or components such as switches or follower circuits.

[0083] This invention discloses a fluorescent algae analysis system. Figure 1 This is a schematic diagram of the structure of a fluorescent algae analysis system according to an embodiment of the present invention. Figure 2 This is a schematic diagram of the composition of a fluorescent algae analysis system in one embodiment of the present invention; please refer to [link / reference]. Figure 1 , Figure 2 The fluorescent algae analysis system includes: a light source driving circuit 1, a turbidity light source 2, several fluorescent light sources 3, a reference detector 4, a fluorescence detector 5, a turbidity detector 6, and a data calculation module 7.

[0084] The light source driving circuit 1 is connected to the turbidity light source 2 and each fluorescent light source 3 respectively, and is used to drive the operation of the turbidity light source 2 and each fluorescent light source 3. The reference detector 4 is used to detect the light intensity data of the turbidity light source or the set fluorescent light source, and send the light intensity data to the light source driving circuit 1. The light source driving circuit 1 adjusts the current supplied to the turbidity light source or the set fluorescent light source so that the turbidity light source or the set fluorescent light source reaches a stable state.

[0085] When the turbidity light source reaches a stable state, the light emitted by the turbidity light source 2 penetrates the water body to be tested and reaches the turbidity detector 6, which detects the turbidity of the water body to be tested.

[0086] When the fluorescent light source reaches a stable state, the light emitted by the fluorescent light source 3 penetrates the water body to be tested and excites the fluorescence of different algae and / or chlorophyll; the fluorescence detector 5 is used to detect the fluorescence data of different intensities.

[0087] The data calculation module 7 is used to obtain algal analysis data by calculating the turbidity data and various fluorescence data. In one embodiment of the present invention, the data calculation module is used to obtain data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a by calculating the turbidity data and various fluorescence data.

[0088] In one embodiment of the present invention, the data calculation module 7 is used to perform turbidity calculation and algae calculation. In one embodiment, the turbidity calculation process includes: firstly, calibration is performed before leaving the factory, and turbidity samples of a set standard concentration are used for measurement (e.g., using several standard concentrations of turbidity samples such as 1 NTU and 100 NTU for measurement), to obtain the relationship between the light intensity measured by the turbidity detector and the corresponding concentration, turbidity = K * turbidity detector light intensity + B; in actual measurement, the turbidity data can be obtained by substituting the detector data.

[0089] The influence coefficient of turbidity on the excitation light source is K = X * turbidity + B; the influence coefficient of turbidity on the fluorescence effect is k = x * turbidity + b; where X, B, x, and b are constants obtained by laboratory measurements. In actual measurements, K and k can be calculated by obtaining the turbidity value.

[0090] The algae calculation process includes: different algae are numbered 1, 2, 3, ..., N, corresponding to different excitation light source numbers a, b, c, ..., n. Taking the first light source a as an example, the relationship between fluorescence and each algae is measured as Ia*K=k*(C1*I1+C2*I2+C3*I3+...+CN*IN). Where K and k are compensation coefficients obtained from turbidity; C is the content of a certain algae; I is the fluorescence effect coefficient of a certain algae, which is a constant; and Ia is the fluorescence produced by light source a for all algae, i.e., the fluorescence intensity measured by the fluorescence detector. By sequentially measuring light sources b, c, d, ..., n, a polynomial combination is obtained, and the content of each algae, C1, C2, C3, ..., CN, is calculated by solving the equation.

[0091] Please continue reading. Figure 1 In one embodiment of the present invention, the fluorescent algae analysis system further includes optical devices, including a first lens 11, a second lens 12, a first semi-transparent mirror 13, and a second semi-transparent mirror 14.

[0092] The turbidity light source 2 transmits part of the light to the reference detector 4 through the first semi-transparent and semi-reflective mirror 13 and the second semi-transparent and semi-reflective mirror 14. The first lens 11 is provided between the reference detector and the second semi-transparent and semi-reflective mirror 14. The turbidity light source 2 transmits part of the light through the water sample to be tested to the turbidity detector 4 through the first semi-transparent and semi-reflective mirror 13 and the second semi-transparent and semi-reflective mirror 14.

[0093] The fluorescent light source 3 transmits part of the light to the reference detector 4 through the first semi-transparent and semi-reflective mirror 13 and the second semi-transparent and semi-reflective mirror 14; the fluorescent light source 3 transmits part of the light through the water body to be tested to the fluorescent detector 5 through the first semi-transparent and semi-reflective mirror 13 and the second semi-transparent and semi-reflective mirror 14, and the second lens 12 is provided between the fluorescent detector 5 and the water body to be tested.

[0094] Specifically, the turbidity light source 2, the first semi-transparent mirror 13, and the second semi-transparent mirror 14 are arranged sequentially from top to bottom above the water body to be tested. The fluorescence light source 3 is arranged on one side of the first semi-transparent mirror 13 (as shown on the left), the turbidity detector 4 is arranged on one side of the second semi-transparent mirror 14 (as shown on the right), and the first lens 11 is arranged between the reference detector and the second semi-transparent mirror 14. The fluorescence detector 5 is arranged on one side of the water body to be tested (as shown on the left), and the second lens 12 is arranged between the fluorescence detector 5 and the water body to be tested. The turbidity detector 6 is arranged below the water body to be tested. Of course, the positions of each component can also be set in other ways, as needed, which will not be elaborated here.

[0095] Figure 3 This is a circuit diagram of a light source driving circuit in one embodiment of the present invention; please refer to [link / reference]. Figure 3 In one embodiment of the present invention, the light source driving circuit includes a first operational amplifier U1A, a first operational amplifier U1B, a first MOS transistor Q1, a second MOS transistor Q2, a third MOS transistor Q3, a first diode D1, a second capacitor C2, a first resistor R1, a second resistor R2, a fourth resistor R4, a fifth resistor R5, and a seventh resistor R7.

[0096] The first end of the fifth resistor R5 receives the first control signal, and the second end of the fifth resistor R5 is connected to the first end of the second capacitor C2 and the non-inverting input terminal of the first operational amplifier U1A, respectively. The second end of the second capacitor C2 is grounded.

[0097] The inverting input terminal of the first operational amplifier U1A is connected to the drain of the second MOSFET Q2 and the first terminal of the seventh resistor R7, with the second terminal of the seventh resistor R7 grounded. The output terminal of the first operational amplifier U1A is connected to the gate of the second MOSFET Q2; the gate of the second MOSFET Q2 is connected to the non-inverting input terminal of the first operational amplifier U1B and the second terminal of the fourth resistor R4.

[0098] The first terminal of the fourth resistor R4 is connected to the power supply voltage and the first terminal of the first resistor R1. The second terminal of the first resistor R1 is connected to the first terminal of the second resistor R2 and the inverting input terminal of the first operational amplifier U1B. The output terminal of the first operational amplifier U1B is connected to the gate of the first MOSFET Q1. The source of the first MOSFET Q1 is connected to the second terminal of the second resistor R2. The drain of the first MOSFET Q1 is connected to the source of the third MOSFET Q3 and the anode of the first diode D1. The gate of the third MOSFET Q3 is connected to the second control signal, and the drain of the third MOSFET Q3 and the cathode of the first diode D1 are grounded.

[0099] In one embodiment of the present invention, an 8-channel light source driving circuit is used; all 8 light source drivers are identical, and their current and flicker frequency are individually controllable. The circuit consists of a constant current source circuit composed of two operational amplifier stages. D1 is the LED light source, MCU_DAC1 is the voltage value output by the microcontroller used to control the LED current value, set to 0-2.5 volts corresponding to 0-25 mA, and MCU_LED1 is the microcontroller output I / O to control the LED's on / off state, used to modulate the flicker frequency of the light source.

[0100] The working principle is as follows: MCU_DAC1 is set to voltage U. According to the "virtual short, virtual open" principle, the voltage across R7 is also U. The voltage across R4 = R4 / R7 * U = the voltage across R1. Therefore, the current flowing through the LED light source D1 is I = (R4 / R7 * U) / R1. Thus, the desired current depends only on the MCU's set voltage U. When MCU_LED1 outputs a high level, MOSFET Q3 is turned on, and all current flows through Q3 to ground. No current flows through D1, and the LED light source is off. When MCU_LED1 outputs a low level, MOSFET Q3 is turned off, and all current flows through D1 to ground. No current flows through Q3, and the LED light source is on. Because the light source switch does not directly control the power supply switch, but rather controls the current path, the output current of the entire constant current source will not change abruptly with the light source switch, resulting in a more stable current and a more stable luminous intensity.

[0101] The circuit drives the turbidity light source, and the reference detector measures the light intensity of the turbidity light source through a semi-transparent and semi-reflective mirror. After the light source driving current is adjusted in real time to reach a stable state, the light penetrates the water body to be tested and reaches the turbidity detector to test the turbidity of the water body.

[0102] The circuit sequentially drives 1 to 7 fluorescent light sources. After reaching a stable state with the reference detector, the light penetrates the water body to be tested and excites the fluorescence of different algal chlorophyll. The fluorescence detector sequentially measures the fluorescence of different intensities excited by the 7 light sources.

[0103] like Figure 3 As shown, the MCU_DAC outputs an initial voltage, such as 1V, which corresponds to a current of 10mA on the LED light source. The expected light intensity measured on the reference detector is 30000. However, in actual operation, due to factors such as light source aging, temperature changes, and structural deformation, the light intensity may deviate from the target value of 30000. In this case, the voltage of the MCU_DAC can be adjusted to control the current to increase or decrease, thereby stabilizing the light intensity obtained on the reference detector at the expected value of 30000.

[0104] After the light source scanning is completed, data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a can be obtained by calculating the turbidity data and seven fluorescence data points. The directly obtained data are the light intensity data measured by the turbidity detector and the fluorescence detector, and the calculated data are the turbidity of the water and the content of each algae.

[0105] Figure 4 This is a circuit diagram of a reference detection circuit in one embodiment of the present invention; please refer to [link / reference]. Figure 4 In one embodiment of the present invention, the reference detection circuit includes a second operational amplifier U2A, a reference detector D2, a first capacitor C1, a third capacitor C3, a third resistor R3, and a sixth resistor R6; the reference detector D2 includes a second diode.

[0106] The non-inverting input of the second operational amplifier U2A is grounded, and the inverting input of the second operational amplifier U2A is connected to the negative terminal of the reference detector D2, the first terminal of the first capacitor C1, and the first terminal of the third resistor R3, respectively; the positive terminal of the reference detector D2 is grounded.

[0107] The output terminal of the second operational amplifier U2A is connected to the second terminal of the first capacitor C1, the second terminal of the third resistor R3, and the first terminal of the sixth resistor R6, respectively; the second terminal of the sixth resistor R6 is connected to the first terminal of the third capacitor C3, and the second terminal of the third capacitor C3 is grounded.

[0108] The reference detection circuit consists of a current-to-voltage conversion circuit composed of a reference detector D2 and an operational amplifier U2A. It converts the light signal emitted by the LED light source into a voltage signal, which is then acquired by the MCU_ADC and the voltage U output by the DAC is finely adjusted in real time to stabilize the light source.

[0109] Figure 5 This is a circuit diagram of a turbidity detection circuit in one embodiment of the present invention; please refer to [link / reference]. Figure 5In one embodiment of the present invention, the turbidity detection circuit includes a third detector D3, a current-to-voltage conversion circuit, a high-pass filter circuit, and a first differential amplifier circuit; the third detector D3 includes a third diode.

[0110] The current-to-voltage conversion circuit includes a third operational amplifier U3A, a fourth capacitor C4, and an eighth resistor R8. The non-inverting input of the third operational amplifier U3A is grounded, and the inverting input of the third operational amplifier U3A is connected to the negative terminal of the third detector D3, the first terminal of the fourth capacitor C4, and the first terminal of the eighth resistor R8. The positive terminal of the third detector D3 is grounded. The output of the third operational amplifier U3A is connected to the second terminal of the fourth capacitor C4 and the second terminal of the eighth resistor R8.

[0111] The high-pass filter circuit includes a fourth operational amplifier U4A, a fifth capacitor C5, a seventh capacitor C7, a ninth resistor R9, a first resistor R11, a third zero resistor R30, and a third first resistor R31.

[0112] The first end of the fifth capacitor C5 is connected to the output terminal of the third operational amplifier U3A. The second end of the fifth capacitor C5 is connected to the first end of the ninth resistor R9 and the first end of the seventh capacitor C7. The second end of the seventh capacitor C7 is connected to the second end of the first resistor R11 and the non-inverting input terminal of the fourth operational amplifier U4A. The first end of the first resistor R11 is grounded.

[0113] The inverting input terminal of the fourth operational amplifier U4A is connected to the second terminal of the third zero resistor R30 and the first terminal of the third first resistor R31, respectively; the first terminal of the third zero resistor R30 is grounded; the output terminal of the fourth operational amplifier U4A is connected to the second terminal of the ninth resistor R9 and the second terminal of the third first resistor R31, respectively.

[0114] The first differential amplifier circuit includes a fifth operational amplifier U5A, a sixth capacitor C6, an eighth capacitor C8, a first zero resistor R10, a first second resistor R12, a first third resistor R13, a first fourth resistor R14, and a first fifth resistor R15.

[0115] The inverting input of the fifth operational amplifier U5A is connected to the second terminal of the first two resistors R12, the first terminal of the first zero resistor R10, and the first terminal of the sixth capacitor C6. The first terminal of the first two resistors R12 is connected to the output of the fourth operational amplifier U4A. The non-inverting input of the fifth operational amplifier U5A is connected to the second terminal of the first four resistors R14 and the first terminal of the first five resistors R15. The first terminal of the first four resistors R14 is connected to the power supply voltage, and the second terminal of the first five resistors R15 is grounded. The output of the fifth operational amplifier U5A is connected to the second terminal of the first zero resistor R10, the second terminal of the sixth capacitor C6, and the first terminal of the first three resistors R13. The second terminal of the first three resistors R13 is connected to the first terminal of the eighth capacitor C8, and the second terminal of the eighth capacitor C8 is grounded.

[0116] The detector D3 and the flow-voltage conversion circuit convert the light from the turbidity light source that has passed through the water sample into a voltage signal. The high-pass filter circuit can filter out low-frequency interference signals and ambient light signals. The differential amplifier circuit amplifies and boosts the signal. The turbidity of the water sample can be calculated by measuring the voltage signal intensity by the MCU_ADC.

[0117] Figure 6 This is a circuit diagram of the fluorescence detection circuit in one embodiment of the present invention; please refer to [link / reference]. Figure 6 In one embodiment of the present invention, the fluorescence detection circuit includes a fourth detector D4, a current-to-voltage conversion circuit, an inverting amplifier circuit, an inverting adder circuit, an integrator circuit, a temperature acquisition circuit, and a second differential amplifier circuit; the fourth detector D4 includes a fourth diode.

[0118] The current-to-voltage conversion circuit includes a seventh operational amplifier U7A, a first capacitor C11, and a first six-resistor R16. The inverting input of the seventh operational amplifier U7A is connected to the negative terminal of the fourth detector D4, the first terminal of the first capacitor C11, and the first terminal of the first six-resistor R16, respectively. The positive terminal of the fourth detector D4 is grounded. The non-inverting input of the seventh operational amplifier U7A is grounded, and the output of the seventh operational amplifier U7A is connected to the second terminal of the first capacitor C11 and the second terminal of the first six-resistor R16, respectively.

[0119] The inverting amplifier circuit includes an eighth operational amplifier U8A, a first capacitor C12, a first resistor R17, and a first resistor R19. The inverting input of the eighth operational amplifier U8A is connected to the second terminal of the first resistor R19, the first terminal of the first capacitor C12, and the first terminal of the first resistor R17, respectively. The first terminal of the first resistor R19 is connected to the output of the seventh operational amplifier U7A. The non-inverting input of the eighth operational amplifier U8A is grounded. The output of the eighth operational amplifier U8A is connected to the second terminal of the first capacitor C12 and the second terminal of the first resistor R17, respectively.

[0120] The inverting adder circuit includes a third operational amplifier U3B, a first three-capacitor C13, a first eight-resistor R18, and a second zero-resistance R20. The inverting input of the third operational amplifier U3B is connected to the second terminal of the second zero-resistance R20, the first terminal of the first three-capacitor C13, and the first terminal of the first eight-resistance R18. The first terminal of the second zero-resistance R20 is connected to the output of an eighth operational amplifier U8A. The non-inverting input of the third operational amplifier U3B is grounded, and the output of the third operational amplifier U3B is connected to the second terminal of the first three-capacitor C13 and the second terminal of the first eight-resistance R18.

[0121] The integrating circuit includes a fourth operational amplifier U4B, a sixth switch U6A, a sixth switch U6B, a first fourth capacitor C14, a first sixth capacitor C16, a second first resistor R21, and a second second resistor R22.

[0122] The first end of the second resistor R21 is connected to the output terminal of the third operational amplifier U3B, and the second end of the second resistor R21 is connected to the first end of the sixth switch U6A. The second end of the sixth switch U6A is connected to the first end of the sixth switch U6B, the first end of the first four capacitors C14, and the inverting input terminal of the fourth operational amplifier U4B. The non-inverting input terminal of the fourth operational amplifier U4B is grounded, and the output terminal of the fourth operational amplifier U4B is connected to the second end of the sixth switch U6B, the second end of the first four capacitors C14, and the first end of the second resistor R22. The second end of the second resistor R22 is connected to the first end of the first six capacitors C16, and the second end of the first six capacitors C16 is grounded.

[0123] The temperature acquisition circuit includes a ninth operational amplifier U9A, a second sixth resistor R26, and a second ninth temperature sensor R29. The non-inverting input of the ninth operational amplifier U9A is connected to the second terminal of the second sixth resistor R26 and the first terminal of the second ninth temperature sensor R29, respectively; the first terminal of the second sixth resistor R26 is connected to the power supply voltage, and the second terminal of the second ninth temperature sensor R29 is grounded; the inverting input of the ninth operational amplifier U9A is connected to the output of the ninth operational amplifier U9A.

[0124] The second differential amplifier circuit includes a fifth operational amplifier U5B, a first capacitor C17, a second resistor R23, a second resistor R24, a second resistor R25, a second resistor R27, and a second resistor R28. The non-inverting input of the fifth operational amplifier U5B is connected to the second terminal of the second resistor R24 ​​and the first terminal of the second resistor R25; the first terminal of the second resistor R24 ​​is connected to the power supply voltage, and the second terminal of the second resistor R25 is grounded. The inverting input of the fifth operational amplifier U5B is connected to the second terminal of the second resistor R27, the first terminal of the second resistor R28, and the first terminal of the first capacitor C17; the first terminal of the second resistor R27 is connected to the output of the ninth operational amplifier U9A. The output of the fifth operational amplifier U5B is connected to the second terminal of the second resistor R28, the second terminal of the first capacitor C17, and the second terminal of the second resistor R23; the first terminal of the second resistor R23 is connected to the inverting input of the third operational amplifier U3B.

[0125] The detector D4 and the flow-voltage conversion circuit convert the fluorescence signal excited by algae in the water sample into a voltage signal. The secondary inverting amplifier circuit amplifies the signal once. The temperature sensor R29 is a PT1000, which divides the voltage with resistor R26. When the temperature changes, it will generate a voltage change. The differential amplifier circuit composed of U5B amplifies and boosts the signal according to the calibrated ratio.

[0126] The subsequent inverting adder circuit adds the fluorescence voltage signal and temperature voltage signal from the preceding stage to eliminate the influence of water temperature on the fluorescence response. The fluorescence effect of algae decreases with increasing temperature, exhibiting a negative temperature coefficient, while the resistance of the PT1000 increases with increasing temperature, exhibiting a positive temperature coefficient. Through amplification and adjustment by the U5B operational amplifier, the error caused by the weakening fluorescence effect with increasing temperature can be compensated.

[0127] The integrating circuit controls analog switch U6A via Ctrl_1 to conduct when the LED light source is on and fluorescence is excited, integrating the pre-processed voltage signal. When the LED light source is off, analog switch U6A is also off to avoid interference. The control logic of analog switch U6B is the same as that of U6A, but in opposite phase: when the LED light source is on, U6B is off, and the integrating circuit integrates normally; when the LED light source is off, U6B conducts, quickly dissipating the charge accumulated on the integrating capacitor C14, preparing for the next integration. The two analog switches work together to enable the integrating circuit to extract the fluorescence signal more accurately.

[0128] The MCU collects fluorescence signals from seven different light sources and turbidity signals from one source. Through calculation, data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a can be obtained.

[0129] This invention further discloses an analytical method for the above-mentioned fluorescent algae analysis system. Figure 7 This is a flowchart of a fluorescent algae analysis method in one embodiment of the present invention; please refer to [link / reference]. Figure 7 The analysis method includes:

[0130]

Step S1

[0131]

Step S2

[0132]

Step S3

[0133]

Step S4

[0134]

Step S5

[0135] In one embodiment, the data calculation module calculates turbidity data and various fluorescence data to obtain data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a.

[0136] In one embodiment of the present invention, the data calculation module performs turbidity calculation and algae calculation. In one embodiment, the turbidity calculation process includes: firstly, calibration is performed before leaving the factory, and turbidity samples of a set standard concentration are used for measurement (e.g., using several standard concentrations of turbidity samples such as 1 NTU and 100 NTU for measurement), to obtain the relationship between the light intensity measured by the turbidity detector and the corresponding concentration, turbidity = K * turbidity detector light intensity + B; during actual measurement, the turbidity data can be obtained by substituting the detector data.

[0137] The influence coefficient of turbidity on the excitation light source is K = X * turbidity + B; the influence coefficient of turbidity on the fluorescence effect is k = x * turbidity + b; where X, B, x, and b are constants obtained by laboratory measurements. In actual measurements, K and k can be calculated by obtaining the turbidity value.

[0138] The algae calculation process includes: different algae are numbered 1, 2, 3, ..., N, corresponding to different excitation light source numbers a, b, c, ..., n. Taking the first light source a as an example, the relationship between fluorescence and each algae is measured as Ia*K=k*(C1*I1+C2*I2+C3*I3+...+CN*IN). Where K and k are compensation coefficients obtained from turbidity; C is the content of a certain algae; I is the fluorescence effect coefficient of a certain algae, which is a constant; and Ia is the fluorescence produced by light source a for all algae, i.e., the fluorescence intensity measured by the fluorescence detector. By sequentially measuring light sources b, c, d, ..., n, a polynomial combination is obtained, and the content of each algae, C1, C2, C3, ..., CN, is calculated by solving the equation.

[0139] This invention also discloses an electronic device, Figure 8 This is a schematic diagram of the composition of an electronic device according to an embodiment of the present invention; please refer to [link / reference]. Figure 8 At the hardware level, the electronic device includes a memory, a processor, and at least one communication interface; the processor may be a microprocessor, and the memory may include main memory, such as random access memory (RAM) or non-volatile memory. Of course, the electronic device may also include other hardware as needed.

[0140] The processor, communication interface, and memory can be interconnected via an internal bus. The memory stores programs (including operating system programs and application programs); the programs may include program code, which may include computer operation instructions. The memory may include main memory and non-volatile memory, and provides instructions and data to the processor.

[0141] In one embodiment, the processor can read the corresponding program from non-volatile memory into memory and then run it; the processor can execute the program stored in memory and specifically perform the following operations (e.g. Figure 7 As shown):

[0142]

Step S1

[0143]

Step S2

[0144]

Step S3

[0145]

Step S4

[0146]

Step S5

[0147] In one embodiment, the data calculation module calculates turbidity data and various fluorescence data to obtain data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a.

[0148] This invention further discloses a storage medium storing computer program instructions, which, when executed by a processor, implement the following steps of the method of this invention (e.g. Figure 7 As shown):

[0149]

Step S1

[0150]

Step S2

[0151]

Step S3

[0152]

Step S4

[0153]

Step S5

[0154] In one embodiment, the data calculation module calculates turbidity data and various fluorescence data to obtain data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a.

[0155] In summary, the fluorescent algae analysis system and method proposed in this invention can improve data stability and accuracy, and eliminate the influence of turbidity on measurement results. In one application scenario of this invention, the driving circuit drives eight different light sources respectively, modulates the driving current and scintillation frequency, and a dedicated acquisition circuit compensates for the stability of the light sources; the fluorescence acquisition circuit is specially designed with temperature compensation, which is directly completed by the hardware circuit without additional calculation, greatly improving data stability and accuracy; the added turbidity compensation function eliminates the influence of turbidity on the measurement results.

[0156] It should be noted that this application can be implemented in software and / or a combination of software and hardware; for example, it can be implemented using an application-specific integrated circuit (ASIC), a general-purpose computer, or any other similar hardware device. In some embodiments, the software program of this application can be executed by a processor to implement the steps or functions described above. Similarly, the software program of this application (including related data structures) can be stored in a computer-readable recording medium; for example, RAM memory, magnetic or optical drives, floppy disks, and similar devices. In addition, some steps or functions of this application can be implemented in hardware; for example, as circuitry that cooperates with a processor to perform the various steps or functions.

[0157] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0158] The description and application of the present invention herein are illustrative and not intended to limit the scope of the invention to the embodiments described above. Effects or advantages involved in the embodiments may not be apparent due to various factors, and the description of effects or advantages is not intended to limit the embodiments. Variations and modifications of the embodiments disclosed herein are possible, and various substitutions and equivalents of the components in the embodiments are well known to those skilled in the art. It should be apparent to those skilled in the art that the invention can be implemented in other forms, structures, arrangements, proportions, and with other components, materials, and parts without departing from the spirit or essential characteristics of the invention. Other variations and modifications can be made to the embodiments disclosed herein without departing from the scope and spirit of the invention.

Claims

1. A fluorescent algae analysis system, characterized in that, The fluorescent algae analysis system includes: a light source driving circuit, a turbidity light source, several fluorescent light sources, a reference detector, a fluorescence detector, a turbidity detector, and a data calculation module; The light source driving circuit is connected to the turbidity light source and each fluorescent light source respectively, and is used to drive the operation of the turbidity light source and each fluorescent light source. The reference detector is used to detect the light intensity data of the turbidity light source or the set fluorescent light source, and send the light intensity data to the light source driving circuit. The light source driving circuit adjusts the current supplied to the turbidity light source or the set fluorescent light source so that the turbidity light source or the set fluorescent light source reaches a stable state. When the turbidity light source reaches a stable state, the light emitted by the turbidity light source penetrates the water body to be tested and reaches the turbidity detector, and the turbidity detector detects the turbidity of the water body to be tested. When the fluorescent light source reaches a stable state, the light emitted by the fluorescent light source penetrates the water body to be tested, exciting the fluorescence of different algae and / or chlorophyll; the fluorescence detector is used to detect the fluorescence data of different intensities. The data calculation module is used to obtain algae analysis data by calculating turbidity data and various fluorescence data.

2. The fluorescent algae analysis system according to claim 1, characterized in that: The data calculation module is used to perform turbidity calculation and algae calculation; The turbidity calculation process includes: initial calibration before leaving the factory, measurement using a turbidity sample of a set standard concentration to obtain the relationship between the light intensity measured by the turbidity detector and the corresponding concentration, turbidity = K * turbidity detector light intensity + B; during actual measurement, the detector data is substituted to obtain the turbidity data; The influence coefficient of turbidity on the excitation light source is K = X * turbidity + B; the influence coefficient of turbidity on the fluorescence effect is k = x * turbidity + b; Among them, the four coefficients X, B, x, and b are measured in the laboratory and are constants. In actual measurement, K and k can be calculated by obtaining the turbidity value. The algae calculation process includes: different algae are numbered 1, 2, 3, ..., N, corresponding to different excitation light source numbers a, b, c, ..., n. The first light source a is measured, and the relationship between fluorescence and each algae is measured as Ia*K=k*(C1*I 1+C2*I2+C3*I3+...+CN*IN). Where K and k are compensation coefficients obtained from turbidity; C is the content of a certain type of algae; I is the fluorescence effect coefficient of a certain type of algae, which is a constant; Ia is the fluorescence produced by light source a for all algae excited by light source a, that is, the fluorescence intensity measured by the fluorescence detector; A system of equations was obtained by measuring the light sources b, c, ..., n in sequence. The content of each algae, C1, C2, C3, ..., CN, was calculated by solving the system of equations.

3. The fluorescent algae analysis system according to claim 1, characterized in that: The fluorescent algae analysis system further includes optical components, which include a first lens, a second lens, a first semi-transparent mirror, and a second semi-transparent mirror. The turbidity light source transmits part of the light to the reference detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror. The first lens is provided between the reference detector and the second semi-transparent and semi-reflective mirror. The turbidity light source transmits part of the light through the water sample to be tested to the turbidity detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror. The fluorescent light source transmits part of the light to the reference detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror; the fluorescent light source transmits part of the light through the water body to be tested to the fluorescent detector through the first semi-transparent and semi-reflective mirror and the second semi-transparent and semi-reflective mirror, and the second lens is provided between the fluorescent detector and the water body to be tested. The data calculation module is used to calculate data on cyanobacteria, green algae, diatoms, dinoflagellates, cryptophytes, and chlorophyll a by calculating turbidity data and various fluorescence data.

4. The fluorescent algae analysis system according to claim 1, characterized in that: The light source driving circuit includes a first operational amplifier U1A, a first operational amplifier U1B, a first MOSFET Q1, a second MOSFET Q2, a third MOSFET Q3, a first diode D1, a second capacitor C2, a first resistor R1, a second resistor R2, a fourth resistor R4, a fifth resistor R5, and a seventh resistor R7. The first terminal of the fifth resistor R5 receives the first control signal, and the second terminal of the fifth resistor R5 is connected to the first terminal of the second capacitor C2 and the non-inverting input terminal of the first operational amplifier U1A, respectively. The second terminal of the second capacitor C2 is grounded. The inverting input terminal of the first operational amplifier U1A is connected to the drain of the second MOS transistor Q2 and the first terminal of the seventh resistor R7, respectively, and the second terminal of the seventh resistor R7 is grounded. The output terminal of the first operational amplifier U1A is connected to the gate of the second MOS transistor Q2; the source terminal of the second MOS transistor Q2 is connected to the non-inverting input terminal of the first operational amplifier U1B and the second terminal of the fourth resistor R4, respectively. The first end of the fourth resistor R4 is connected to the power supply voltage and the first end of the first resistor R1, and the second end of the first resistor R1 is connected to the first end of the second resistor R2 and the inverting input terminal of the first B operational amplifier U1B. The output terminal of the first operational amplifier U1B is connected to the gate of the first MOS transistor Q1, the source of the first MOS transistor Q1 is connected to the second terminal of the second resistor R2, and the drain of the first MOS transistor Q1 is connected to the source of the third MOS transistor Q3 and the positive terminal of the first diode D1, respectively. The gate of the third MOS transistor Q3 is connected to the second control signal, and the drain of the third MOS transistor Q3 and the cathode of the first diode D1 are grounded respectively.

5. The fluorescent algae analysis system according to claim 1, characterized in that: The reference detection circuit includes a second operational amplifier U2A, a reference detector D2, a first capacitor C1, a third capacitor C3, a third resistor R3, and a sixth resistor R6; the reference detector D2 includes a second diode; The non-inverting input of the second operational amplifier U2A is grounded, and the inverting input of the second operational amplifier U2A is connected to the negative terminal of the reference detector D2, the first terminal of the first capacitor C1, and the first terminal of the third resistor R3, respectively; the positive terminal of the reference detector D2 is grounded. The output terminal of the second operational amplifier U2A is connected to the second terminal of the first capacitor C1, the second terminal of the third resistor R3, and the first terminal of the sixth resistor R6, respectively; the second terminal of the sixth resistor R6 is connected to the first terminal of the third capacitor C3, and the second terminal of the third capacitor C3 is grounded.

6. The fluorescent algae analysis system according to claim 1, characterized in that: The turbidity detection circuit includes a third detector D3, a current-to-voltage conversion circuit, a high-pass filter circuit, and a first differential amplifier circuit; the third detector D3 includes a third diode; The current-to-voltage conversion circuit includes a third operational amplifier U3A, a fourth capacitor C4, and an eighth resistor R8. The non-inverting input of the third operational amplifier U3A is grounded, and the inverting input of the third operational amplifier U3A is connected to the negative terminal of the third detector D3, the first terminal of the fourth capacitor C4, and the first terminal of the eighth resistor R8. The positive terminal of the third detector D3 is grounded. The output of the third operational amplifier U3A is connected to the second terminal of the fourth capacitor C4 and the second terminal of the eighth resistor R8. The high-pass filter circuit includes a fourth operational amplifier U4A, a fifth capacitor C5, a seventh capacitor C7, a ninth resistor R9, a first resistor R11, a third zero resistor R30, and a third first resistor R31. The first end of the fifth capacitor C5 is connected to the output terminal of the third operational amplifier U3A. The second end of the fifth capacitor C5 is connected to the first end of the ninth resistor R9 and the first end of the seventh capacitor C7. The second end of the seventh capacitor C7 is connected to the second end of the first resistor R11 and the non-inverting input terminal of the fourth operational amplifier U4A. The first end of the first resistor R11 is grounded. The inverting input terminal of the fourth operational amplifier U4A is connected to the second terminal of the third zero resistor R30 and the first terminal of the third first resistor R31, respectively; the first terminal of the third zero resistor R30 is grounded; the output terminal of the fourth operational amplifier U4A is connected to the second terminal of the ninth resistor R9 and the second terminal of the third first resistor R31, respectively. The first differential amplifier circuit includes a fifth operational amplifier U5A, a sixth capacitor C6, an eighth capacitor C8, a first zero resistor R10, a first second resistor R12, a first third resistor R13, a first fourth resistor R14, and a first fifth resistor R15; The inverting input terminal of the fifth operational amplifier U5A is connected to the second terminal of the first two resistors R12, the first terminal of the first zero resistor R10, and the first terminal of the sixth capacitor C6, respectively; the first terminal of the first two resistors R12 is connected to the output terminal of the fourth operational amplifier U4A. The non-inverting input terminal of the fifth operational amplifier U5A is connected to the second terminal of the first four resistors R14 and the first terminal of the first five resistors R15, respectively; the first terminal of the first four resistors R14 is connected to the power supply voltage, and the second terminal of the first five resistors R15 is grounded. The output terminal of the fifth operational amplifier U5A is connected to the second terminal of the first zero resistor R10, the second terminal of the sixth capacitor C6, and the first terminal of the first three resistors R13, respectively; the second terminal of the first three resistors R13 is connected to the first terminal of the eighth capacitor C8, and the second terminal of the eighth capacitor C8 is grounded.

7. The fluorescent algae analysis system according to claim 1, characterized in that: The fluorescence detection circuit includes a fourth detector D4, a current-to-voltage conversion circuit, an inverting amplifier circuit, an inverting adder circuit, an integrating circuit, a temperature acquisition circuit, and a second differential amplifier circuit; the fourth detector D4 includes a fourth diode; The current-to-voltage conversion circuit includes a seventh operational amplifier U7A, a first capacitor C11, and a first six resistor R16; the inverting input terminal of the seventh operational amplifier U7A is connected to the negative terminal of the fourth detector D4, the first terminal of the first capacitor C11, and the first terminal of the first six resistor R16, respectively, and the positive terminal of the fourth detector D4 is grounded. The non-inverting input terminal of the seventh operational amplifier U7A is grounded, and the output terminal of the seventh operational amplifier U7A is connected to the second terminal of the first capacitor C11 and the second terminal of the first resistor R16, respectively. The inverting amplifier circuit includes an eighth operational amplifier U8A, a first second capacitor C12, a first seventh resistor R17, and a first ninth resistor R19; The inverting input terminal of the eighth operational amplifier U8A is connected to the second terminal of the first nine resistor R19, the first terminal of the first two capacitors C12, and the first terminal of the first seven resistor R17, respectively; the first terminal of the first nine resistor R19 is connected to the output terminal of the seventh operational amplifier U7A. The non-inverting input terminal of the eighth operational amplifier U8A is grounded; the output terminal of the eighth operational amplifier U8A is connected to the second terminal of the first capacitor C12 and the second terminal of the first resistor R17, respectively. The inverting adder circuit includes a third operational amplifier U3B, a first three-capacitor C13, a first eight-resistor R18, and a second zero-resistor R20. The inverting input terminal of the third operational amplifier U3B is connected to the second terminal of the second zero resistor R20, the first terminal of the first three capacitors C13, and the first terminal of the first eight resistors R18, respectively; the first terminal of the second zero resistor R20 is connected to the output terminal of the eighth operational amplifier U8A. The non-inverting input terminal of the third operational amplifier U3B is grounded, and the output terminal of the third operational amplifier U3B is connected to the second terminal of the first three capacitors C13 and the second terminal of the first eight resistors R18, respectively. The integrating circuit includes a fourth operational amplifier U4B, a sixth switch U6A, a sixth switch U6B, a first fourth capacitor C14, a first sixth capacitor C16, a second first resistor R21, and a second second resistor R22. The first end of the second resistor R21 is connected to the output terminal of the third operational amplifier U3B, and the second end of the second resistor R21 is connected to the first end of the sixth switch U6A. The second terminal of the sixth A switch U6A is connected to the first terminal of the sixth B switch U6B, the first terminal of the first four capacitors C14, and the inverting input terminal of the fourth B operational amplifier U4B, respectively. The non-inverting input terminal of the fourth operational amplifier U4B is grounded, and the output terminal of the fourth operational amplifier U4B is connected to the second terminal of the sixth switch U6B, the second terminal of the first four capacitors C14, and the first terminal of the second two resistors R22, respectively; the second terminal of the second two resistors R22 is connected to the first terminal of the first six capacitors C16, and the second terminal of the first six capacitors C16 is grounded. The temperature acquisition circuit includes a ninth operational amplifier U9A, a second sixth resistor R26, and a second ninth temperature sensor R29. The non-inverting input terminal of the ninth operational amplifier U9A is connected to the second terminal of the second six resistor R26 and the first terminal of the second nine temperature sensor R29, respectively; the first terminal of the second six resistor R26 is connected to the power supply voltage, and the second terminal of the second nine temperature sensor R29 is grounded; the inverting input terminal of the ninth operational amplifier U9A is connected to the output terminal of the ninth operational amplifier U9A. The second differential amplifier circuit includes a fifth operational amplifier U5B, a first seventh capacitor C17, a second third resistor R23, a second fourth resistor R24, a second fifth resistor R25, a second seventh resistor R27, and a second eighth resistor R28. The non-inverting input terminal of the fifth operational amplifier U5B is connected to the second terminal of the second fourth resistor R24 ​​and the first terminal of the second fifth resistor R25, respectively; the first terminal of the second fourth resistor R24 ​​is connected to the power supply voltage, and the second terminal of the second fifth resistor R25 is grounded. The inverting input terminal of the fifth operational amplifier U5B is connected to the second terminal of the second seven resistor R27, the first terminal of the second eight resistor R28, and the first terminal of the first seven capacitor C17, respectively; the first terminal of the second seven resistor R27 is connected to the output terminal of the ninth operational amplifier U9A. The output terminal of the fifth operational amplifier U5B is connected to the second terminal of the second eight resistor R28, the second terminal of the first seven capacitor C17, and the second terminal of the second three resistor R23, respectively; the first terminal of the second three resistor R23 is connected to the inverting input terminal of the third operational amplifier U3B.

8. An analytical method for the fluorescent algae analysis system according to any one of claims 1 to 7, characterized in that, The analytical method includes: The light source driving circuit drives the turbidity light source to work; the reference detector detects the light intensity data of the turbidity light source and sends the light intensity data to the light source driving circuit; the light source driving circuit adjusts the current supplied to the turbidity light source so that the turbidity light source or the set fluorescent light source reaches a stable state. When the turbidity light source reaches a stable state, the light emitted by the turbidity light source penetrates the water body to be tested and reaches the turbidity detector, and the turbidity detector detects the turbidity of the water body to be tested. The light source driving circuit sequentially drives the operation of each fluorescent light source; the reference detector detects the light intensity data of the set fluorescent light source and sends the light intensity data to the light source driving circuit, and the light source driving circuit adjusts the current supplied to the set fluorescent light source so that the turbidity light source or the set fluorescent light source reaches a stable state. When the fluorescent light source reaches a stable state, the light emitted by the fluorescent light source penetrates the water body to be tested, exciting the fluorescence of different algae and / or chlorophyll; the fluorescence detector detects the fluorescence data of different intensities. The data calculation module calculates algae analysis data by analyzing turbidity data and various fluorescence data.

9. An electronic device comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, characterized in that, When the processor executes the computer program, it implements the steps of the method of claim 8.

10. A storage medium storing computer program instructions thereon, characterized in that, When the computer program instructions are executed by the processor, they implement the steps of the method of claim 8.

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