Cultivation method of scirpus mariqueter

Abstract

According to the scirpus mariqueter cultivation method, in the cultivation process, adopted cultivation soil comprises sandy soil, yellow brown soil and nutrient soil. In the scirpus mariqueter cultivation process, the mixed soil composed of the sandy soil, the yellow brown soil and the nutrient soil is selected as the cultivation soil, the air permeability of the yellow brown soil can be improved through the sandy soil, and the water-retaining property of the sandy soil is improved through the yellow brown soil; sodium ions and ions in salt are adsorbed through the ion exchange effect of basic groups and acid groups contained in humus in the nutrient soil, damage of salt in sandy soil to scirpus mariqueter is reduced, the germination rate of the scirpus mariqueter is increased, and the growth state of plants is improved.

Description

Technical Field

[0001] This invention relates to the field of planting technology, and in particular to a method for cultivating sea sedge. Background Technology

[0002] *Scirpus triqueter*, a plant belonging to the genus *Scirpus* in the family Cyperaceae, is a perennial emergent or wetland herb resulting from a hybrid of *Scirpus scutellatus* and *Scirpus scutellatus*. The stems of *Scirpus triqueter* are 25-40 cm tall, with corms and creeping rhizomes coexisting, and a well-developed root system. *Scirpus triqueter* is endemic to China, distributed in Hebei, Jiangsu, and the Yangtze River estuary. It is characterized by its salt tolerance, flood tolerance, siltation tolerance, resistance to wind and wave impact, vigorous vitality, and strong reproductive capacity. *Scirpus triqueter* plays a role in promoting siltation and shoal formation, soil stabilization, soil improvement, wave reduction, beach protection, and sediment settling. It provides habitats for many marine organisms, contributing to the maintenance of biodiversity in the Yangtze River estuary. Its corms are rich in starch, serving as a wintering food source for migratory birds such as swans and wild ducks.

[0003] In recent years, due to the combined effects of intensive reclamation in the Yangtze River estuary, large-scale introduction of the invasive species Spartina alterniflora, a sharp decrease in sediment load from the middle and upper reaches of the Yangtze River, and sea-level rise caused by global warming, the area of ​​*Scirpus triqueter* is shrinking at an accelerated pace. If corresponding measures are not taken in time, *Scirpus triqueter* will face the threat of extinction.

[0004] In related technologies, the seedling cultivation of *Scirpus triqueter* involves soil cultivation, artificial irrigation, and growth under natural light. However, this method results in a low germination rate, poor plant growth, and a tendency for the plants to wither and die. Summary of the Invention

[0005] This invention provides a method for cultivating *Scirpus triquetrum* to solve the above-mentioned technical problems.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: This invention provides a method for cultivating *Scirpus triqueter*, wherein the cultivation soil used in the cultivation process includes sandy soil, yellow-brown soil, and nutrient soil.

[0007] In one embodiment of the present invention, the mass ratio of the sand, the yellow-brown soil and the nutrient soil is 63-68:28-32:4-6, preferably 63-67:29-32:5-6.

[0008] In one embodiment of the present invention, the cultivation is carried out in March or April when the outdoor temperature is 3-10°C, preferably in March or April when the outdoor temperature is 4-10°C.

[0009] In one embodiment of the present invention, during the cultivation process, the temperature of the environment in which the seeds or plants of *Scirpus triqueter* are located is 12-16°C, preferably 12-15°C.

[0010] In one embodiment of the present invention, during the cultivation process, the relative humidity of the environment in which the seeds or plants of *Rubus parvifolius* are located is 70%RH-80%RH, preferably 70%RH-78%RH.

[0011] In one embodiment of the present invention, during the seedling stage, the absolute value of the difference between the water level in the cultivation container and the height of the cultivation container is controlled to be less than or equal to 0.3 cm, preferably less than or equal to 0.1 cm.

[0012] In one embodiment of the present invention, during the cultivation process, when the seedlings that have grown out of the seedling soil reach a height of 0.5cm, fresh flowing water is introduced. Every few days, the old flowing water is drained and fresh flowing water is introduced (hereinafter referred to as water recycling).

[0013] In one embodiment of the present invention, the five-day biochemical oxygen demand of the fresh flowing water is 5-19 mg / L, preferably 6-19 mg / L.

[0014] In one embodiment of the present invention, the pH of the fresh flowing water is 7.5-8.5.

[0015] In one embodiment of the present invention, the salt content of the fresh flowing water is 0.3-0.6 g / L, preferably 0.4-0.6 g / L.

[0016] The beneficial effects of this invention are: In the cultivation of *Scirpus triquetrum*, this invention selects a mixed soil composed of sand, yellow-brown soil, and nutrient soil as the cultivation soil. The sand improves the aeration of the yellow-brown soil, the yellow-brown soil improves the water retention of the sand, and the ion exchange of alkaline and acidic groups contained in the humus in the nutrient soil adsorbs sodium ions and other ions from the salt, thereby reducing the damage of salt in the sand to *Scirpus triquetrum*, thus improving the germination rate of *Scirpus triquetrum* and improving the plant's growth status.

[0017] In this invention, by adjusting the mass ratio of sandy soil, yellow-brown soil and nutrient soil to 63-68:28-32:4-6, the permeability and water retention of the cultivation soil can be further guaranteed, thereby improving the germination rate of *Scirpus triqueter* and improving the plant growth status.

[0018] In this invention, during the cultivation process, by setting the temperature of the environment in which the seeds or plants of *Rubus triquetrum* are located at 12-16°C, the effects of high or low temperature stress can be avoided, photosynthesis can be enhanced, thereby increasing the germination rate of *Rubus triquetrum* and improving the plant's growth status.

[0019] In this invention, during the cultivation process, by setting the relative humidity of the environment in which the seeds or plants of *Rubus triquetrum* are located to 70%RH-80%RH, the effect of water stress can be alleviated, thereby improving the germination rate of *Rubus triquetrum* and improving the plant's growth status.

[0020] In this invention, during the cultivation process, once the seedlings that have emerged from the nursery soil reach a height of 0.5 cm, fresh flowing water is introduced. Every few days, the old flowing water is drained and fresh flowing water is introduced. This enhances the efficiency of oxygen exchange between the seedling roots and the seedlings, accelerates the diffusion of nutrients such as nitrogen and phosphorus in the water, promotes the growth of *Scirpus triqueter* plants into the deeper mud, and promotes the absorption of nutrients by *Scirpus triqueter* plants, thus improving the growth status of *Scirpus triqueter* plants. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] One embodiment of the present invention provides a method for cultivating *Scirpus triqueter*, wherein the cultivation soil used in the cultivation process includes sandy soil, yellow-brown soil and nutrient soil.

[0023] This invention uses a cultivation soil consisting of sandy soil, yellow-brown soil, and nutrient soil during the cultivation of *Scirpus triqueter*. The sandy soil improves the aeration of the yellow-brown soil, the yellow-brown soil improves the water retention of the sandy soil, and the nutrient soil absorbs sodium ions and other ions from salts through ion exchange between the alkaline and acidic groups contained in the humus. This reduces the damage of salts in the sandy soil to *Scirpus triqueter*, thereby increasing the germination rate of *Scirpus triqueter* and improving the plant's growth status.

[0024] In one embodiment of the present invention, the mass ratio of sandy soil, yellow-brown soil and nutrient soil is 63-68:28-32:4-6, preferably 63-67:29-32:5-6.

[0025] In this invention, by adjusting the mass ratio of sandy soil, yellow-brown soil and nutrient soil to 63-68:28-32:4-6, the permeability and water retention of the cultivation soil can be further guaranteed, thereby improving the germination rate of *Scirpus triqueter* and improving the plant growth status.

[0026] In one embodiment of the present invention, the cultivation time is March-April when the outdoor temperature is 3-10℃, preferably March-April when the outdoor temperature is 4-10℃.

[0027] In one embodiment of the present invention, during the cultivation process, the temperature of the environment in which the seeds or plants of *Scirpus triqueter* are located is 12-16°C, preferably 12-15°C.

[0028] In this invention, during the cultivation process, by setting the temperature of the environment in which the seeds or plants of *Rubus triquetrum* are located at 12-16°C, the effects of high or low temperature stress can be avoided, photosynthesis can be enhanced, thereby increasing the germination rate of *Rubus triquetrum* and improving the plant's growth status.

[0029] In one embodiment of the present invention, during the cultivation process, the relative humidity of the environment in which the seeds or plants of *Rubus parvifolius* are located is 70%RH-80%RH, preferably 70%RH-78%RH.

[0030] In this invention, during the cultivation process, by setting the relative humidity of the environment in which the seeds or plants of *Rubus triquetrum* are located to 70%RH-80%RH, the effect of water stress can be alleviated, thereby improving the germination rate of *Rubus triquetrum* and improving the plant's growth status.

[0031] In one embodiment of the present invention, during the seedling stage, the absolute value of the difference between the water level in the cultivation container and the height of the cultivation container is controlled to be less than or equal to 0.3 cm, preferably less than or equal to 0.1 cm.

[0032] In this invention, during the seedling stage, by controlling the absolute value of the difference between the water level in the cultivation container and the height of the cultivation container to be less than or equal to 0.3 cm, seed germination and seedling root development can be promoted, thereby improving the plant's growth status.

[0033] In one embodiment of the present invention, during the cultivation process, when the seedlings that have grown out of the seedling soil reach a height of 0.5cm, fresh flowing water is introduced. Every few days, the old flowing water is drained and fresh flowing water is introduced.

[0034] In this invention, during the cultivation process, once the seedlings that have emerged from the nursery soil reach a height of 0.5 cm, fresh flowing water is introduced. Every few days, the old flowing water is drained and fresh flowing water is introduced. This enhances the efficiency of oxygen exchange between the seedling roots and the seedlings, accelerates the diffusion of nutrients such as nitrogen and phosphorus in the water, promotes the growth of *Scirpus triqueter* plants into the deeper mud, and promotes the absorption of nutrients by *Scirpus triqueter* plants, thus improving the growth status of *Scirpus triqueter* plants.

[0035] In one embodiment of the present invention, the five-day biochemical oxygen demand (BOD) of the fresh flowing water is 5-19 mg / L, preferably 6-19 mg / L; the pH of the fresh flowing water is 7.5-8.5; and the salinity of the fresh flowing water is 0.3-0.6 g / L, preferably 0.4-0.6 g / L. It should be noted that if the salinity of the flowing water is below 0.3 g / L, substances such as sodium chloride can be added to bring the salinity of the flowing water to the above-mentioned range.

[0036] Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0037] (a) Selection of potting soil A method for cultivating *Scirpus triquetrum*, the specific steps of which are as follows: S1. Divide the seeds of *Scirpus triquetrum* into four equal parts; S2. Use the following four types of soil as culture media to cultivate *Scirpus triqueter* seeds: Sandy soil (from the coastal area of ​​Chongming Island, Shanghai) was used as the cultivation soil. Yellow-brown soil (from the inland area of ​​Nantong, Jiangsu) was used as the second type of cultivation soil; Sandy soil (from the coastal area of ​​Chongming Island, Shanghai), yellow-brown soil (from the inland area of ​​Nantong, Jiangsu) and nutrient soil (commercially available) were mixed evenly in a mass ratio of 70:25:5 to obtain the third type of cultivation soil; Sandy soil (from the coastal area of ​​Chongming Island, Shanghai), yellow-brown soil (from the inland area of ​​Nantong, Jiangsu) and nutrient soil (commercially available, garden nutrient soil) were mixed evenly in a mass ratio of 65:30:5 to obtain the fourth type of cultivation soil. The above four types of potting soil were filled into four seedling trays (each with 8*16 holes, a length of 54cm, a width of 28cm, a single hole diameter of 3.0cm, a height of 4.0cm, and a bottom diameter of 1.2cm). In late March, when the outdoor temperature is 3-10℃ (maximum temperature 10℃, minimum temperature 3℃), scatter 8 seeds of *Scirpus triqueter* into the middle of the four types of potting soil as described above, then cover with another layer of potting soil, cover with a thin film to cover the seedling tray, and place the seedling tray in the nursery; then cover with another thin film to cover the nursery. Open the second layer of film (the film covering the seedling bed) at noon each day and close it at night to control the temperature inside the second layer of film at 12-16℃ and the relative humidity at 70%RH-78%RH. When the seedlings that have emerged from the seedling soil reach a height of 0.5cm, remove the first layer of film (the film covering the seedling trays) and introduce fresh running water, ensuring the water level is 2cm below the seedling trays. Thereafter, drain the old running water and introduce fresh running water every four days. Specifically, the fresh running water is river water from a tributary of the Yangtze River flowing into the Chongming area (the river water has a pH of 7.5-8.5 and a five-day biochemical oxygen demand (BOD) of 5-19 mg / L; because the salinity of the river water is below 0.3g / L, sodium chloride is added to bring the salinity to 0.3g / L). Test the water each time it is used; pH is measured using a pH meter, and the five-day BOD is measured according to the HJ standard. The water quality standard is 505-2009 "Determination of Five-Day Biochemical Oxygen Demand (BOD5) in Water - Dilution and Inoculation Method". The salinity standard is 51-2024 "Determination of Total Salinity in Water - Gravimetric Method" (hereinafter the same). When the germination is just beginning, apply urea to the cultivation area at a dosage of 10 mg / m³. 2 ; After 23 days, the germination rate, growth rate, root and stem growth value (i.e., root and stem length), and seedling and leaf growth value (i.e., seedling and leaf length) of each group were counted. The results are shown in Table 1.

[0038] Table 1. Results of the screening test for the potting soil

[0039] Note: Germination rate and growth rate data are approximate values.

[0040] Table 1 shows that compared with culture soil 1 and culture soil 2, the germination rate, seedling growth value, and growth rate of *Scirpus triqueter* were significantly increased when cultured using culture soil 3 and culture soil 4. This result indicates that, in the cultivation of *Scirpus triqueter*, the present invention, by selecting a mixed soil composed of sandy soil, yellow-brown soil, and nutrient soil as the culture soil, can improve the aeration of the yellow-brown soil through the sandy soil, improve the water retention of the sandy soil through the yellow-brown soil, and reduce the damage of salt in the sandy soil to *Scirpus triqueter* through the ion exchange of alkaline and acidic groups contained in the humus in the nutrient soil, thereby increasing the germination rate and improving the plant's growth status.

[0041] Table 1 shows that, compared with culture soil 3, the germination rate, rhizome growth value, seedling and leaf growth value, and growth rate of *Scirpus triqueter* were significantly increased when cultured in culture soil 4. This result indicates that, in this invention, adjusting the mass ratio of sandy soil, yellow-brown soil, and nutrient soil to 63-68:28-32:4-6 further ensures the aeration and water retention of the culture soil, thereby improving the germination rate of *Scirpus triqueter* and enhancing the plant's growth status.

[0042] (ii) Screening based on temperature and relative humidity A method for cultivating *Scirpus triquetrum*, the specific steps of which are as follows: S1. Using the above-mentioned culture soil four as the culture soil, cultivate the seeds of *Scirpus triqueter*: Four seedling trays (8*16 holes, 54cm in length, 28cm in width, 3.0cm in diameter per hole, 4.0cm in height and 1.2cm in bottom diameter) were labeled A, B, C and D respectively, and the above-mentioned potting soil was filled into each of the four seedling trays. In late March, when the outdoor temperature is 3-10℃ (maximum 10℃, minimum 3℃), 8 seeds of *Scirpus triqueter* were sown into the center of the corresponding group's potting soil in seedling tray A. A layer of potting soil was then added to level with the tray, and a thin film was placed over it. The tray was then placed in a nursery bed. Another thin film was added to cover the nursery bed. The second film (covering the nursery bed) was opened at midday each day and closed in the evening to maintain a temperature of 8-10℃ and a relative humidity of 50%RH-60%RH within the second film. When the seedlings emerged from the potting soil and reached a height of 0.5cm, the first film (covering the seedling tray) was removed, and fresh running water was introduced (as above) to keep the water level 2cm below the seedling tray. Thereafter, every 4 days, the old running water was drained and fresh running water was introduced (as above). When the germination is just beginning, apply urea to the cultivation area at a dosage of 10 mg / m³. 2 This group is recorded as Group A; The difference between seedling tray B and seedling tray A is that the timing is in mid-March when the outdoor temperature is 5-10℃ (the highest temperature is 10℃ and the lowest temperature is 5℃), and the ambient temperature inside the second layer of film is controlled at 12-16℃ and the relative humidity is 70%RH-78%RH. This group is recorded as group C. The difference between seedling tray C and seedling tray A is that the timing is in mid-March when the outdoor temperature is 3-10℃ (the highest temperature is 3℃ and the lowest temperature is 10℃), without covering it with two layers of film, that is, using the outdoor environment as the cultivation environment. At this time, the relative humidity is 65%RH-75%RH, and this group is recorded as group C. The difference between seedling tray D and seedling tray A is that the timing is early April when the outdoor temperature is 10-15℃ (maximum temperature is 15℃, minimum temperature is 10℃), and the ambient temperature inside the second layer of film is controlled at 16-22℃ and the relative humidity is 60%RH-70%RH. This group is recorded as group C. After 23 days, the growth rate, root and stem growth value (i.e., root and stem length), and seedling leaf growth value (i.e., seedling leaf length) were statistically analyzed, and the results are shown in Table 2.

[0043] Table 2. Results of temperature and humidity screening tests

[0044] Note: Germination rate and growth rate data in the table are approximate values.

[0045] Table 2 shows that compared with groups A, C, and D, group B exhibited significantly improved root and stem growth values, seedling and leaf growth values, and growth rate. This result indicates that, in this invention, setting the temperature of the environment where *Scirpus triqueter* seeds or plants are located during cultivation at 12-16℃ can avoid high or low temperature stress, enhance photosynthesis, thereby increasing the germination rate of *Scirpus triqueter* and improving plant growth. Furthermore, setting the relative humidity of the environment where *Scirpus triqueter* seeds or plants are located during cultivation at 70%RH-80%RH can alleviate water stress, thereby increasing the germination rate of *Scirpus triqueter* and improving plant growth.

[0046] (III) Screening of water and water level A method for cultivating *Scirpus triquetrum*, the specific steps of which are as follows: S1. Using the above-mentioned culture soil four as the culture soil, cultivate the seeds of *Scirpus triqueter*: Six seedling trays (8*16 holes, 54cm in length, 28cm in width, 3.0cm in diameter at the opening of each hole, 4.0cm in height and 1.2cm in diameter at the bottom) were labeled E, F, G, H, I and J respectively. The above-mentioned potting soil was filled into each of the six seedling trays. In late March, when the outdoor temperature is 5-10℃ (maximum temperature 10℃, minimum temperature 5℃), scatter 8 seeds of *Scirpus triqueter* into the center of the corresponding group of seedling trays in the potting soil, cover with another layer of potting soil, cover with a thin film, place the seedling trays in the nursery, and then cover with another thin film to cover the nursery. Open the second layer of film (the film covering the seedling bed) at noon every day and close the second layer of film (the film covering the seedling bed) every evening to control the temperature inside the second layer of film at 12-16℃ and the relative humidity at 70%RH-78%RH. When the seedlings that have grown out of the seedling soil reach a height of 0.5cm, remove the first layer of film (the film covering the seedling tray). Fresh running water is introduced into seedling tray E (as above) to raise the water level 2cm above the seedling tray. Thereafter, every 4 days, the old running water is drained and fresh running water is introduced (as above). When the germination is just beginning, apply urea to the cultivation area at a dosage of 10 mg / m³. 2 This group is recorded as Group E; The difference between group F of seedling trays and group F of seedling trays is that the water level is 2cm lower than that of the seedling trays. This group is recorded as group F. The difference between seedling tray G and seedling tray E is that the water level in seedling tray G is level with the water level in seedling tray E. This group is recorded as group G. The difference between seedling tray H and seedling tray E is that the old running water was not drained and fresh running water was introduced every 4 days. That is, after the first introduction of fresh running water, the water was not circulated. This group is recorded as group H. The difference between seedling tray I and seedling tray F is that the old running water was not drained and fresh running water was introduced every 4 days. That is, after the first introduction of fresh running water, the water was not circulated. This group is recorded as group I. The difference between seedling tray J and seedling tray G is that the old running water was not drained and fresh running water was introduced every 4 days. That is, after the first introduction of fresh running water, the water was not circulated. This group is recorded as group J. After 23 days, the growth rate, root and stem growth value (i.e., root and stem length), and seedling leaf growth value (i.e., seedling leaf length) were statistically analyzed, and the results are shown in Table 3.

[0047] Table 3 Results of water and water level screening tests

[0048] Note: Germination rate and growth rate data in the table are approximate values.

[0049] Table 3 shows that compared with group H, group E exhibited significantly improved growth rate, root and stem growth value, and seedling and leaf growth value; compared with group I, group F exhibited significantly improved growth rate, root and stem growth value, and seedling and leaf growth value; and compared with group J, group G exhibited significantly improved growth rate, root and stem growth value, and seedling and leaf growth value. These results indicate that in this invention, during the cultivation process, once the seedlings reach a height of 0.5 cm above the nursery soil, fresh flowing water is introduced. Every few days, the old flowing water is drained and fresh flowing water is introduced, which enhances the efficiency of oxygen exchange between the seedling roots and the plant, accelerates the diffusion of nutrients such as nitrogen and phosphorus in the water, promotes the growth of *Scirpus triqueter* plants into deeper mud, and improves the absorption of nutrients by *Scirpus triqueter* plants, thus improving their growth status.

[0050] As shown in Table 3, compared with groups E and F, group G showed significantly improved growth rate, root and stem growth value, and seedling and leaf growth value. This result indicates that, in the seedling stage, controlling the absolute value of the difference between the water level and the height of the cultivation container to be less than or equal to 0.3 cm can promote seed germination, promote seedling root development, and thus improve plant growth.

[0051] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A method for cultivating *Scirpus triquetrum*, characterized in that, The cultivation soil used in the process includes sandy soil, yellow-brown soil and nutrient soil.

2. The method for cultivating *Scirpus triquetrum* as described in claim 1, characterized in that, The mass ratio of the sand, the yellow-brown soil and the nutrient soil is 63-68:28-32:4-6.

3. The method for cultivating *Scirpus triquetrum* as described in claim 1, characterized in that, The cultivation period is from March to April when the outdoor temperature is 3-10℃.

4. The method for cultivating *Scirpus triquetrum* as described in claim 3, characterized in that, During the cultivation process, the temperature of the environment in which the seeds or plants of *Rubus parvifolius* are located is 12-16℃.

5. The method for cultivating *Scirpus triquetrum* as described in claim 3, characterized in that, During the cultivation process, the relative humidity of the environment in which the seeds or plants of *Rubus parvifolius* are located is 70%-80%RH.

6. The method for cultivating *Scirpus triquetrum* as described in claim 1, characterized in that, During the seedling stage, the absolute value of the difference between the water level in the cultivation container and the height of the cultivation container is controlled to be less than or equal to 0.3 cm.

7. The method for cultivating *Scirpus triquetrum* as described in claim 1, characterized in that, During the cultivation process, once the seedlings that have grown out of the nursery soil reach a height of 0.5cm, fresh running water is introduced. Every few days, the old running water is drained and fresh running water is introduced.

8. The method for cultivating *Scirpus triquetrum* as described in claim 7, characterized in that, The five-day biochemical oxygen demand of the fresh flowing water is 5-19 mg / L.

9. The method for cultivating *Scirpus triquetrum* as described in claim 7, characterized in that, The pH of the fresh, flowing water is 7.5-8.

5.

10. The method for cultivating *Scirpus triquetrum* as described in claim 7, characterized in that, The salt content of the fresh flowing water is 0.3-0.6 g / L.

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