Liquorice and smoked plum repairing composition and application

Abstract

The invention discloses a licorice and dark plum repairing composition and application, and belongs to the technical field of medicine. The liquorice and smoked plum repairing composition is prepared from a liquorice water decoction extract and a smoked plum water decoction extract in a mass ratio of (0.6-1.2): 1. The composition can synergistically protect GES-1 cells infected and damaged by helicobacter pylori, significantly improve the cell activity and improve the cell morphological structure integrity, and has good helicobacter pylori resistance activity and gastric mucosa protection effect. In MTT cell activity detection, the survival rate of GES-1 cells under HP infection can be remarkably and synergistically improved. The liquorice and smoked plum repairing composition shows obvious proportion dependence in different proportion tests, when the proportion of the liquorice water decoction extract and the smoked plum water decoction extract is controlled within the set range, the synergistic effect is most remarkable, and when the proportion deviates from the range, the synergistic effect is remarkably reduced.

Description

A licorice and plum repair composition and its application Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a licorice and plum repair composition and its application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Helicobacter pylori (HP) infection is a major causative factor of gastric diseases such as chronic gastritis and peptic ulcers. Its pathogenic process stems not only from the colonization and reproduction of the bacteria themselves, but more importantly, from the damage it causes to the gastric mucosal barrier. This damage mechanism includes direct invasion of gastric mucosal epithelial cells, induction of local inflammatory responses and oxidative stress, ultimately leading to cell apoptosis, barrier function disruption, and potentially progression to gastric cancer. Currently, the standard first-line treatment in clinical practice is triple or quadruple therapy, the core of which is the eradication of bacteria using antibiotics. However, such regimens have two major problems: first, the increasingly serious problem of antibiotic resistance leads to a higher eradication failure rate; second, while antibiotics kill bacteria, they cannot effectively and directly repair the damaged gastric mucosa, and the improvement of patient symptoms and histological healing are often asynchronous. Therefore, developing drugs or compositions that can target and protect against and repair gastric mucosal epithelial cell damage caused by Helicobacter pylori is of significant clinical importance for improving disease prognosis and compensating for the shortcomings of existing therapies.

[0004] In the search for gastric mucosal protectants, traditional Chinese medicine and its active ingredients have shown promising potential. Existing technologies have confirmed that various Chinese herbal extracts possess anti-inflammatory, antioxidant, and cell-repair-promoting effects. For example, glycyrrhizic acid and other components in the decoction extract of licorice have been shown to have significant anti-inflammatory and cell-protective activities, inhibiting the release of inflammatory factors and alleviating oxidative stress, thereby reducing damage to the gastric mucosa from various irritants to some extent. As a traditional Chinese medicine, the decoction extract of dried plum is rich in organic acids and other components, and studies have shown that it can exert a protective effect on the mucosa through antioxidant and immunomodulatory pathways. However, current technologies have not revealed whether the combination of licorice and dried plum interacts in gastric mucosal protection, particularly regarding damage to gastric mucosal epithelial cells (such as GES-1 cells) under a specific pathological model of Helicobacter pylori infection. Whether the combination of the two can produce a synergistic repair effect remains a blank. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a licorice and plum repair composition and its application. The inventors have discovered that the combination of licorice decoction extract and plum decoction extract exhibits a significant synergistic effect at the cellular level. This combination can effectively reduce HP damage to GES-1 cells, improve cell viability, and enhance cell morphological integrity, demonstrating good anti-HP potential.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: In the first aspect of the present invention, a licorice and plum repair composition is provided, comprising a decoction extract of licorice and a decoction extract of plum, wherein the mass ratio of the decoction extract of licorice and the decoction extract of plum is 0.6-1.2:1.

[0007] Licorice is the dried root and rhizome of *Glycyrrhiza uralensis* Fisch., *Glycyrrhiza inflata* Bat., or *Glycyrrhiza glabra* L., all belonging to the legume family. It is a commonly used traditional Chinese medicine with the effects of clearing heat and detoxifying, relieving spasms and pain, and harmonizing other herbs. Modern pharmacological studies have shown that the active ingredients in the decoction extract of licorice, such as glycyrrhizic acid and glycyrrhizin flavonoids, have anti-inflammatory, antioxidant, antibacterial, and gastric mucosal protective effects. It can alleviate mucosal damage caused by *Helicobacter pylori* infection by regulating gastric epithelial cell signaling pathways. Ume (*Prunus mume* Sieb. et Zucc.) is the immature fruit of *Prunus mume*, a plant in the Rosaceae family, processed through smoking and drying. It is rich in citric acid, malic acid, succinic acid, gallic acid, and polyphenolic compounds, and has antibacterial, anti-inflammatory, astringent, urease-inhibiting, and gastric mucosal-protective effects. The Compendium of Materia Medica records that dried plum can "soothe the intestines, relieve intestinal gas, and stop diarrhea," and modern research has also confirmed its inhibitory effect on *Helicobacter pylori* (HP) growth. Through research, the inventors discovered that the combination of licorice root decoction extract and dried plum decoction extract exhibits a significant synergistic effect at the cellular level. This combination can synergistically improve the survival rate of GES-1 cells, effectively reduce HP damage to GES-1 cells, enhance cell viability, and improve cell morphological integrity, demonstrating good anti-HP potential. The licorice root and dried plum repair composition provided by this invention not only significantly improves efficacy but also reduces drug resistance and the risk of adverse reactions, possessing significant clinical and application value.

[0008] In some embodiments of the present invention, the mass ratio of licorice decoction extract to plum decoction extract is 0.6:1, 0.67:1, 1:1, or 1.2:1. Within this range, the synergistic effect of the antibacterial activity of licorice decoction extract and plum decoction extract is more significant.

[0009] This invention does not specify the preparation method for licorice decoction extract and plum decoction extract; commonly used preparation methods in the field are acceptable.

[0010] In some embodiments of the present invention, the preparation method of the licorice decoction extract includes: mixing licorice rhizome with water at a material-to-liquid ratio of 1:(9-11), boiling in water for 0.5-1.5 hours, and filtering to obtain a first extract; mixing the residue with water at a material-to-liquid ratio of 1:(7-9), boiling in water for 0.5-1.5 hours, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure and freeze-drying to obtain a pure licorice decoction extract.

[0011] Preferably, the preparation method of the licorice decoction extract includes: mixing licorice root and water at a ratio of 1:10, boiling in water for 1 hour, and filtering to obtain a first extract; mixing the residue and water at a ratio of 1:8, boiling in water for 1 hour, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure and freeze-drying to obtain a pure licorice decoction extract.

[0012] In some embodiments of the present invention, the preparation method of the plum decoction extract includes: mixing plum with water at a material-to-liquid ratio of 1:(9-11), boiling in water for 0.5-1.5 hours, and filtering to obtain a first extract; mixing the residue with water at a material-to-liquid ratio of 1:(7-9), boiling in water for 0.5-1.5 hours, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure and freeze-drying to obtain a pure licorice decoction extract.

[0013] Preferably, the preparation method of the ume decoction extract includes: mixing ume and water at a material-to-liquid ratio of 1:10, boiling in water for 1 hour, and filtering to obtain a first extract; mixing the residue and water at a material-to-liquid ratio of 1:8, boiling in water for 1 hour, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure and freeze-drying to obtain a pure licorice decoction extract.

[0014] In some embodiments of the present invention, the licorice and plum repair composition can be prepared by the following method: take licorice decoction extract and plum decoction extract, dissolve them separately in pure water to form mother liquor, mix them in proportion, stir to dissolve evenly, concentrate under reduced pressure or freeze dry directly to obtain the licorice and plum repair composition.

[0015] The licorice and plum repair composition provided by this invention can synergistically improve the survival rate of GES-1 cells and effectively reduce cell damage caused by Helicobacter pylori infection, demonstrating excellent synergistic protective effects.

[0016] A second aspect of the present invention provides the use of the licorice and plum repair composition described in the first aspect in the preparation of a treatment for gastric epithelial cell damage.

[0017] In some embodiments of the present invention, the gastric epithelial cell damage includes any one or more of the damage caused by Helicobacter pylori infection and damage caused by gastrointestinal diseases.

[0018] In some embodiments of the present invention, the Helicobacter pylori infection includes any one or more of acute HP infection and chronic HP infection.

[0019] In some embodiments of the present invention, the gastrointestinal diseases include any one or more of chronic superficial gastritis, atrophic gastritis, gastric ulcer, gastric mucosa-associated lymphoid tissue lymphoma (MALT lymphoma), and precancerous lesions of the stomach.

[0020] In some embodiments of the present invention, the licorice and plum repair composition synergistically protects GES-1 cells damaged by Helicobacter pylori infection. Its mechanism of action includes: (1) reducing the cytotoxic damage of Helicobacter pylori to GES-1 cells; (2) improving the morphological and structural integrity of infected cells; and (3) increasing the survival rate of GES-1 cells, thereby achieving the effect of protecting gastric epithelial cells and improving the gastric mucosal barrier function.

[0021] A third aspect of the present invention provides a medicament for treating gastric epithelial cell damage, comprising a pharmaceutically acceptable carrier or excipient and the licorice-prunus repair composition described in the first aspect.

[0022] In some embodiments of the present invention, the pharmaceutically acceptable carrier includes, but is not limited to, any one or more of lactose, starch, microcrystalline cellulose, kaolin, sucrose, talc, gelatin, agar, pectin, gum arabic, magnesium stearate, stearic acid, syrup, peanut oil, olive oil, and water.

[0023] In some embodiments of the present invention, the excipients include, but are not limited to, any one or more of the following: preservatives, colorants, diluents, granulators, emulsifiers, lubricants, binders, disintegrants, buffer solutions, flavoring agents, surfactants, thickeners, fillers, absorption promoters, and surfactants.

[0024] In some embodiments of the present invention, the dosage form of the drug is selected from any one or more of powder, pill, capsule, granule, tablet or oral liquid.

[0025] In some embodiments of the invention, tablets containing the licorice and plum repair composition of the invention can be prepared by compression or molding, optionally using one or more excipients or adjuvants. The active ingredient can be compressed in a free-flowing form (e.g., powder or granules) in a suitable machine, optionally mixed with binders, lubricants, inert diluents, surfactants, or dispersants. Molded tablets can be molded in a suitable machine, i.e., a mixture of powdered compounds moistened with an inert liquid diluent. Each tablet preferably contains about 0.05 mg to about 5 g of the active ingredient, and each sachet or capsule preferably contains about 0.05 mg to about 5 g of the active ingredient. For example, a formulation intended for oral administration to humans may contain about 0.5 mg to about 5 g of the active drug, mixed with a suitable and convenient carrier material, which may comprise about 5% to 95% of the total composition. Unit dosage forms typically contain approximately 1 mg to approximately 2 g of active ingredient, usually in doses of 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg, or 1000 mg.

[0026] In some embodiments of the present invention, the drug for treating gastric epithelial cell damage is a solution, and the total concentration of licorice decoction extract and plum decoction extract in the solution is 0.55-1.25 g / L.

[0027] In some embodiments of the present invention, the concentration of licorice decoction extract in the solution is 0.3-0.5 g / L, and the concentration of plum decoction extract is 0.25-0.75 g / L.

[0028] In some embodiments of the present invention, the preparation method of the solution includes dissolving licorice decoction extract and plum decoction extract in a co-solvent (e.g., ultrapure water), mixing them evenly, and then diluting them with a pharmaceutically acceptable solvent to obtain the solution.

[0029] The preparation process can be carried out at room temperature. After mixing, the solution is filtered through a 0.22 μm filter membrane for sterilization to obtain a clear solution. The resulting solution can be used directly in in vitro experiments or further prepared into a pharmaceutical composition for oral administration.

[0030] The beneficial effects of this invention are as follows: The licorice and plum repair composition provided by this invention can synergistically protect GES-1 cells damaged by Helicobacter pylori infection, significantly improve cell viability, improve cell morphological structure integrity, and have good anti-Helicobacter pylori activity and gastric mucosal protection effect. It is expected to be developed into a drug or health care product with better efficacy and higher safety for the treatment or prevention of Helicobacter pylori infection and related gastric diseases.

[0031] This invention unexpectedly discovered and confirmed that the combination of licorice decoction extract and plum decoction extract has a significant synergistic protective effect in repairing gastric mucosal epithelial cell (GES-1) damage caused by Helicobacter pylori. As shown in Figure 2, after Helicobacter pylori infection, the model group (Figure 2b) showed extensive cell damage. Although the use of licorice decoction extract alone (Figure 2c) or plum decoction extract alone (Figure 2d) both showed some protective effect, the combined use of the two (Figure 2e) showed the most outstanding effect, with the best recovery of cell morphology and density, closest to the normal control group. This result clearly indicates that the combination of the two produces a synergistic effect of "1+1>2". Moreover, this licorice and plum repair composition realizes a shift from simple "antibacterial" to a multi-target treatment strategy that emphasizes both "antibacterial" and "mucosal repair", providing a better solution for the core pathogenic link of Helicobacter pylori (damage to the gastric mucosa). Existing therapies (such as antibiotics) mainly focus on eradicating bacteria, but are insufficient in repairing the already caused tissue damage. In this invention, the anti-inflammatory and antioxidant components of licorice and the mucosal protective components of dried plum work synergistically to directly act on damaged gastric mucosal epithelial cells, effectively inhibiting cell apoptosis and promoting repair, thereby fundamentally helping to restore the integrity of the gastric mucosal barrier. This is of great value for improving treatment efficacy and reducing recurrence of old diseases. This licorice and dried plum repair composition is derived from medicinal and edible herbs, with high potential safety and broad application prospects. Compared with chemical drugs, licorice and dried plum, as traditional Chinese medicines, have a good safety profile. Through synergistic effects, it is expected to achieve excellent cell protection effects at lower doses, laying a solid foundation for the development of novel gastric mucosal protectants or adjuvant therapies with fewer side effects and higher patient compliance. Attached Figure Description

[0032] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0033] Figure 1 shows the results of the licorice and plum repair composition provided in Example 1 of the present invention in repairing GES-1 cell damage; wherein, GC is licorice decoction extract and WM is plum decoction extract.

[0034] Figure 2 shows the experimental results of the licorice and plum repair composition provided in Example 1 of the present invention improving the survival rate of GES-1 cells; where a is the effect of the control group, b is the effect of the model group, c is the effect of 0.3 g / L licorice decoction extract, d is the effect of 0.5 g / L plum decoction extract, and e is the effect of 0.3 g / L licorice decoction extract + 0.5 g / L plum decoction extract. Detailed Implementation

[0035] To enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0036] The preparation method of the licorice decoction extract used in the following examples is as follows: Licorice rhizome and pure water are mixed at a ratio of 1:10, boiled at 100°C for 1 hour, and filtered to obtain the first extract; the residue is mixed with pure water at a ratio of 1:8, boiled at 100°C for 1 hour, and filtered to obtain the second extract; the two extracts are combined, concentrated under reduced pressure, freeze-dried to remove residual water to obtain the licorice decoction extract.

[0037] The preparation method of the plum decoction extract used in the following examples is as follows: Plum and pure water are mixed at a ratio of 1:10, boiled at 100°C for 1 hour, and filtered to obtain the first extract; the residue is mixed with pure water at a ratio of 1:8, boiled at 100°C for 1 hour, and filtered to obtain the second extract; the two extracts are combined, concentrated under reduced pressure, freeze-dried to remove residual water to obtain the plum decoction extract.

[0038] Example 1: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, wherein the mass ratio of licorice decoction extract to plum decoction extract is 0.6:1.

[0039] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of the licorice decoction extract is 0.3 g / L, the concentration of the plum decoction extract is 0.5 g / L, and the solvent is water.

[0040] Example 2: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, with a mass ratio of licorice decoction extract to plum decoction extract of 0.67:1.

[0041] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of the licorice decoction extract is 0.5 g / L, the concentration of the plum decoction extract is 0.75 g / L, and the solvent is water.

[0042] Example 3: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, with a mass ratio of licorice decoction extract to plum decoction extract of 1:1.

[0043] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of both the licorice decoction extract and the plum decoction extract is 0.5 g / L, and the solvent is water.

[0044] Example 4: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, with a mass ratio of licorice decoction extract to plum decoction extract of 1.2:1.

[0045] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of the licorice decoction extract is 0.3 g / L, the concentration of the plum decoction extract is 0.25 g / L, and the solvent is water.

[0046] Comparative Example 1: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, wherein the mass ratio of licorice decoction extract to plum decoction extract is 2:1.

[0047] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of the licorice decoction extract is 0.5 g / L, the concentration of the plum decoction extract is 0.25 g / L, and the solvent is water.

[0048] Comparative Example 2: A licorice and plum repair composition, consisting of licorice decoction extract and plum decoction extract, wherein the mass ratio of licorice decoction extract to plum decoction extract is 0.5:1.

[0049] This licorice and plum repair composition can be formulated into a solution for treating gastric epithelial cell damage (i.e., a mixed aqueous solution of licorice decoction extract and plum decoction extract). The concentration of the licorice decoction extract is 0.5 g / L, the concentration of the plum decoction extract is 1 g / L, and the solvent is water.

[0050] The solutions for treating gastric epithelial cell injury in Examples 1-4 and Comparative Examples 1 and 2 were prepared by the following method: Quantitative amounts of licorice decoction extract and plum decoction extract were dissolved separately in a small amount of water to obtain licorice decoction extract stock solution and plum decoction extract stock solution. The two stock solutions were mixed evenly according to the specified ratio and diluted to the target concentration to obtain the solution for treating gastric epithelial cell injury.

[0051] Experimental Example: Improvement Effect of Each Group: The improvement effect (E) was calculated using formulas (1) and (2).A =(C A -C M ) / (C0-C m ))(1)E A =(C AB -C M ) / (C0-C m (2) Where C0 represents the cell survival rate of the blank control group, C m C represents the cell survival rate of the model group. A C represents the cell survival rate of the single-drug group. AB E represents cell viability in the combined drug administration group. A E represents the protective effect of a single extract. AB This represents the protective effect of the combination of licorice decoction extract and plum decoction extract.

[0052] The following experiments validated the synergistic effect: the expected summation and drug synergistic coefficient (CI) were calculated using formulas (3) and (4).

[0053] Expected summation effect = E A +E B -E A ×E B (3) CI=(E A +E B -E A ×E B ) / E AB (4) Among them, E A E represents the protective effect of licorice decoction extract. B E represents the protective effect of the decoction extract of dried plum. AB This represents the protective effect of the combination of licorice decoction extract and plum decoction extract. If CI is less than 1, i.e., E... AB The effect was greater than the expected summation, demonstrating a synergistic effect between the decoction extract of licorice and the decoction extract of dried plum.

[0054] Cell viability (MTT) assay: GES-1 cells were seeded in 96-well plates at 5000 cells per well, and RPMI-1640 medium containing 10% fetal bovine serum was added. The cells were cultured at 37°C and 5% CO2 for 24 h. After cell attachment, different test solutions (100 μL per well) were added to each well, and 1×10⁻⁶ MTT assay was performed simultaneously. 6 One or more Helicobacter pylori bacteria were co-cultured for 24 h. After culturing, MTT solution (final concentration 0.05 mg / mL) was added to each well, and incubation continued for 4 h. The culture medium was then discarded, and DMSO was added to dissolve the formazan crystals. The absorbance (OD) was measured at 570 nm using a microplate reader. 570The relative cell viability of the uninfected group was normalized to 1, and the relative cell viability of the other experimental groups was normalized to 1.

[0055] Table 1. Concentration statistics of the examples and comparative examples

[0056] The test results are shown in Table 2 (n=3, mean ± standard deviation, relative to the blank control group as 1).

[0057] Table 2 Test results for each group

[0058] As shown in Figure 2, the CI values ​​of all examples (Examples 1-4) are less than 1, indicating a significant synergistic effect between the licorice decoction extract and the plum decoction extract within the mass ratio range defined in this invention. This significantly improves the survival rate of GES-1 cells infected with Helicobacter pylori and provides synergistic protection against Helicobacter pylori-infected GES-1 cells. The CI values ​​of Comparative Examples 1 and 2 are higher than those of Examples 1-4, indicating that when the mass ratio of the licorice decoction extract to the plum decoction extract deviates from the range set in this invention (0.6-1.2:1), the synergistic effect is significantly weakened or disappears.

[0059] Figure 2a shows the control group, where cells are evenly distributed, morphologically intact, and exhibit typical paving stone-like monolayer adherent growth with intact cell membranes and clear boundaries. This indicates that GES-1 cells are healthy under normal culture conditions, providing an ideal benchmark for this experiment. Figure 2b shows the model group, where cell condition is significantly worse than the control group. Specifically, cell number is reduced, intercellular spaces are increased, cells become rounded, shrunken, and their integrity is compromised. This clearly confirms that Helicobacter pylori attack causes significant damage to GES-1 cells, leading to decreased cell survival and successful model establishment. Figures 2c and 2d show the single-factor treatment groups. Compared to the model group, the cell condition of both single-drug treatment groups is significantly improved, but the number of GES-1 cells is still low and their morphology is poor, indicating a weak protective effect on GES-1 cells. Figure 2e shows the treatment group of the licorice and plum repair composition of the present invention. This group showed the most significant improvement in cell morphology, with well-developed and structurally intact cells. Its cell density, spreading state, and health were significantly better than the model group and superior to any single-drug group, and closest to the control group. This result strongly suggests that the combined use of licorice and plum has a protective effect on cells that surpasses the effect of using either drug alone.

[0060] In summary, the combination of licorice decoction extract and dried plum anti-Helicobacter pylori provided by the present invention can produce significant synergistic protective effects within a specific ratio range, and has good anti-Helicobacter pylori activity and gastric epithelial cell protection effect.

[0061] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A licorice and dried plum repair composition, characterized in that, It includes licorice decoction extract and plum decoction extract, with a mass ratio of 0.6-1.2:

1.

2. The licorice and plum repair composition as described in claim 1, characterized in that, The mass ratio of licorice decoction extract to plum decoction extract is 0.6:1, 0.67:1, 1:1 or 1.2:

1.

3. The licorice and plum repair composition as described in claim 1, characterized in that, The preparation method of the licorice decoction extract includes: mixing licorice root and water at a material-to-liquid ratio of 1:(9-11), boiling in water for 0.5-1.5 hours, and filtering to obtain a first extract; mixing the residue with water at a material-to-liquid ratio of 1:(7-9), boiling in water for 0.5-1.5 hours, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure, and freeze-drying to obtain a pure licorice decoction extract; the preparation method of the plum decoction extract includes: mixing plum with water at a material-to-liquid ratio of 1:(9-11), boiling in water for 0.5-1.5 hours, and filtering to obtain a first extract; mixing the residue with water at a material-to-liquid ratio of 1:(7-9), boiling in water for 0.5-1.5 hours, and filtering to obtain a second extract; combining the two extracts, concentrating under reduced pressure, and freeze-drying to obtain a pure licorice decoction extract.

4. The use of the licorice and plum repair composition according to any one of claims 1-3 in the preparation of a medicament for treating gastric epithelial cell damage.

5. The application as described in claim 4, characterized in that, The gastric epithelial cell damage includes any one or more of the damage caused by Helicobacter pylori infection and the damage caused by gastrointestinal diseases; preferably, the gastrointestinal diseases include any one or more of chronic superficial gastritis, atrophic gastritis, gastric ulcer, gastric mucosa-associated lymphoid tissue lymphoma, and gastric precancerous lesions.

6. A drug for treating gastric epithelial cell damage, characterized in that, Includes pharmaceutically acceptable carriers or excipients and the licorice and plum repair composition according to any one of claims 1-3.

7. The drug as described in claim 6, characterized in that, The pharmaceutically acceptable carrier includes any one or more of lactose, starch, microcrystalline cellulose, kaolin, sucrose, talc, gelatin, agar, pectin, gum arabic, magnesium stearate, stearic acid, syrup, peanut oil, olive oil, and water; preferably, the excipients include any one or more of preservatives, colorants, diluents, granulators, emulsifiers, lubricants, binders, disintegrants, buffer solutions, flavoring agents, surfactants, thickeners, fillers, absorption enhancers, and surfactants.

8. The drug as described in claim 6, characterized in that, The dosage form of the drug is selected from any one or more of the following: powder, pill, capsule, granule, tablet or oral liquid.

9. The drug as described in claim 6, characterized in that, The drug is a solution, and the total concentration of the licorice decoction extract and the plum decoction extract in the solution is 0.55-1.25 g / L.

10. The medicament as claimed in claim 9, characterized in that, In the solution, the concentration of licorice decoction extract is 0.3-0.5 g / L, and the concentration of plum decoction extract is 0.25-0.75 g / L.

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