Suspending agent containing fenaminstrobin and validamycin A and application thereof

Abstract

The invention relates to the field of agricultural bactericides, in particular to a suspending agent containing fenaminstrobin and validamycin A and application of the suspending agent. The bactericidal composition comprises a suspending agent containing fenaminstrobin and validamycin A, an active component, a stabilizer, an auxiliary agent and water, the active component accounts for 1-70% of the mass of the preparation, and the stabilizer accounts for 0.1-10% of the mass of the preparation; wherein the active ingredients are fenaminstrobin and validamycin A, and the stabilizer is meglumine (N-methyl-D-glucosamine). The suspending agent prepared by the technical scheme of the invention effectively inhibits the increase of the particle size of a sample in the thermal storage process of a preparation, effectively improves the suspension rate of fenaminstrobin after the thermal storage of the preparation, improves the utilization rate of a pesticide, and ensures the normal exertion of the efficacy of the pesticide at the same time. Meanwhile, the quality of the active components of the stabilizer-containing bactericide is stable within the shelf life, the addition of the stabilizer does not cause the decomposition of the active components, and the preparation does not have obvious physical or chemical change.

Description

Technical Field

[0001] This invention relates to the field of agricultural fungicides, and particularly to a suspension containing tebufenozide and jinggangmycin A and its application. Background Technology

[0002] Enoximil has a broad-spectrum fungicidal activity, possessing both protective and curative effects. It exhibits good control efficacy against various plant diseases, such as powdery mildew and downy mildew. In terms of its mechanism of action, enoximil inhibits pathogen growth and reproduction by interfering with the normal physiological functions of pathogen cells. Enoximil is characterized by high efficacy, low toxicity, and environmental friendliness. This compound has been disclosed in CN1309897A.

[0003] Jinggangmycin A is an antibiotic fungicide produced by *Streptococcus hygroscopicus*, belonging to the category of biological pesticides. It has strong systemic properties, meaning it can be absorbed by plants and translocated within them. Jinggangmycin A is primarily used to control rice sheath blight. Its mechanism of action is to inhibit the growth of the pathogen's mycelium, thereby achieving the purpose of disease control.

[0004] The long-term and excessive use of chemical fungicides has led to increased resistance in pathogens and increasingly serious pesticide residue problems. To promote green agricultural development in my country, biopesticides, due to their low toxicity, good environmental compatibility, and low likelihood of resistance development, play a positive role in reducing chemical pesticide use and ensuring the safety of agricultural products. However, the number of registered biopesticides in my country is currently limited, and the control effect of using biopesticides alone is not very good. Since biopesticides and chemical pesticides have different mechanisms of action against pathogens, their synergistic use has greater potential for increasing the effectiveness of chemical pesticides while reducing their application.

[0005] Chinese invention patent CN101836631B reports that the combined use of tebuconazole and jinggangmycin has good control effects on rice sheath blight, rice false smut, and wheat sheath blight, with a control rate of over 97% in field trials. It also exhibits strong persistence, with a control rate of over 92% maintained 14 days after application for rice and wheat sheath blight. However, the focus is on the bioactivity of the combined active ingredients, lacking research on screening high-performance pesticide formulations. The specific examples mention a suspension concentrate formulation, but only provide efficacy data for some targets. Suspension concentrates need good physical properties to ensure good physicochemical stability during shelf life. Suspension rate is a key performance indicator for suspension concentrates; according to current standards, a suspension rate of at least 90% is required to indicate successful formulation development. Patent CN103749493A reports problems encountered during the preparation of a suspension concentrate using a combination of jinggangmycin A and thifluzamide, including high viscosity, inability to mill, adhesion to walls during storage, and the production of black flocculent matter. These problems are addressed by adding a special polyether polyol with a low HLB value as an anti-flocculation agent. Patent CN103734139A discloses a pesticide suspension concentrate combining jinggangmycin and hexaconazole. This invention increases the suspension rate by selecting the rust-inhibiting additive α-[tris(phenylmethyl)phenyl]-ω-hydroxy poly(oxy-1,2-ethylene) as a nonionic surfactant and synergistically working with sodium diisooctyl succinate. Patent CN107535488A discloses a pesticide suspension concentrate combining jinggangmycin and flutriafol and its preparation method. This also suffers from low suspension rate and poor stability. This invention improves the suspension rate by using sucrose fatty acid esters and sodium ditridecyl sulfosuccinate to prevent the aggregation of active ingredient particles. Patent CN108651464A discloses a suspension concentrate of a compound fungicide consisting of jinggangmycin A and tebuconazole, and its preparation method. This invention improves the stability of the formulation by adding a high-salt system stabilizer (phenolic resin sulfonate) to inhibit the growth of the active ingredient particle size. During formulation research on the preparation of a suspension concentrate of tebuconazole and jinggangmycin A, it was found that the prepared suspension concentrate dilution was prone to low tebuconazole suspension rate due to particle aggregation, especially in accelerated heat storage tests (54±2℃, 14d). The increased particle size led to unacceptable tebuconazole suspension rate, ultimately affecting the use of the formulation. Currently, no publicly available technology has been reported to address this problem. Summary of the Invention

[0006] To overcome the shortcomings of existing technologies and to address the problem of significant particle size increase and unqualified suspension rate of acetamiprid in accelerated thermal storage tests of compound suspensions containing acetamiprid and jinggangmycin A, this invention provides a suspension containing acetamiprid and jinggangmycin A and its application.

[0007] To achieve the above objectives, the technical solution of the present invention is as follows:

[0008] A suspension containing tebufenozide and jinggangmycin A, comprising an active ingredient, a stabilizer, an adjuvant, and water, wherein the active ingredient accounts for 1-70% of the formulation weight, and the stabilizer accounts for 0.1-10% of the formulation weight; wherein the active ingredient is tebufenozide and jinggangmycin A, and the stabilizer is meglumine (N-methyl-D-glucosamine).

[0009] The amount of acetamiprid is 1-40% by weight of the formulation, and the amount of jinggangmycin A is 1-40% by weight of the formulation.

[0010] Preferably, the oxadiazonamide accounts for 1-10% of the weight of the formulation, and the jinggangmycin A accounts for 1-20% of the weight of the formulation.

[0011] The adjuvant is one or more of the following: wetting and dispersing agent, thickener, defoamer, and antifreeze agent. The components account for 1-20% of the weight of the formulation, 0-2% of the wetting and dispersing agent, 0-2% of the thickener, 0-2% of the defoamer, 0-2% of the preservative, and 0-10% of the antifreeze agent.

[0012] The wetting and dispersing agent is selected from one or more of the following: lignin sulfonates, sodium salts of alkyl naphthalene sulfonate condensates, alkyl naphthalene sulfonate formaldehyde condensates, polycarboxylates, EO / PO block copolymers, phosphate anions, sulfate anions, polyacrylate copolymers, fatty alcohol polyoxyethylene ethers, sorbitan fatty acid ester polyoxyethylene ethers, and methacrylic acid grafted comb copolymers.

[0013] The wetting and dispersing agent is a sodium salt of alkyl naphthalene sulfonic acid condensate and a mixture of one or more of the following substances: lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, polycarboxylate salts, EO / PO block copolymers, phosphate ester anions, sulfate anions, polyacrylate copolymers, fatty alcohol polyoxyethylene ethers, sorbitan fatty acid ester polyoxyethylene ethers, and methacrylic acid grafted comb copolymers.

[0014] The mass ratio of the stabilizer meglumine (N-methyl-D-glucosamine) to the sodium salt of the alkyl naphthalene sulfonic acid condensate in the wetting and dispersing agent is 1:1 to 1:10.

[0015] The thickener is selected from one or more of xanthan gum, bentonite, gelatin, gum arabic, polyvinyl acetate, polyvinylpyrrolidone, magnesium aluminum silicate, polyvinyl alcohol, polyethylene glycol, phenolic resin, shellac, and hydroxymethyl cellulose.

[0016] The defoamer is selected from one or more of SAG-1522, SAG-1572, silicones, C8-10 fatty alcohols, phosphate esters, C10-20 saturated fatty acids (such as decanoic acid) and amides;

[0017] The preservative is selected from sodium benzoate, Kathon (2-methyl-4-isothiazolin-3-one and (B)5-chloro-2-methyl-4-isothiazolin-3-one), potassium sorbate, sodium dehydroacetate or one or more of the following;

[0018] The antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea.

[0019] The application of the suspension containing tebufenozide and jinggangmycin A, wherein the suspension is used as an agricultural fungicide.

[0020] The formulation is applied at a concentration of 10-500 grams of active ingredient per hectare to the pathogens to be controlled or their growth medium. It can be used to control one or more plant diseases, including rice sheath blight, rice blast, wheat powdery mildew, wheat rust, soybean rust, soybean gray spot, cotton damping-off, cotton anthracnose, cucumber powdery mildew, cucumber downy mildew, grape downy mildew, apple leaf spot, apple powdery mildew, banana leaf spot, tomato early blight, strawberry powdery mildew, and rapeseed sclerotinia stem rot.

[0021] Advantages of this invention:

[0022] This invention addresses the problem that, when preparing suspensions from highly water-soluble jinggangmycin A and acetamiprid, the particle size of the formulation samples significantly increases and the suspension rate of acetamiprid decreases after accelerated heat storage (54±2℃, 14d), resulting in substandard performance. By selecting a suitable adjuvant system and incorporating a special stabilizer with a suitable wetting and dispersing agent system into the formulation, this invention solves the problem of significant particle size increase and substandard acetamiprid suspension rate in the accelerated heat storage test of the acetamiprid and jinggangmycin A compound suspension, and effectively improves the suspension rate of the formulation after heat storage.

[0023] The stabilizer meglumine used in this invention has a characteristic spatial configuration of multiple hydroxyl and amino groups, which can interact with acetamiprid drug particles. Through electrostatic repulsion and steric hindrance, it prevents the aggregation and flocculation of acetamiprid particles. This solves the problem of the acetamiprid suspension rate failing the accelerated thermal storage test after acetamiprid and jinggangmycin A suspension.

[0024] Meanwhile, the bactericide containing stabilizer of the present invention has stable quality of active components during the shelf life, and the addition of stabilizer will not cause decomposition of effective ingredients, and the formulation will not undergo significant physical or chemical changes. Detailed Implementation

[0025] The following specific embodiments are used to further illustrate the present invention, but the present invention is by no means limited to these examples. Methods well known to those in the art are all included within the scope of the present invention.

[0026] The suspension prepared using the technical solution of this invention effectively inhibits the increase in sample particle size during the heat storage of the formulation, effectively improves the suspension rate of tebufenozide after heat storage, and enhances pesticide utilization while ensuring the normal efficacy of the pesticide. Furthermore, the fungicide containing stabilizers of this invention maintains stable quality of its active components throughout its shelf life; the addition of stabilizers does not cause decomposition of the active ingredients, and the formulation does not undergo significant physical or chemical changes.

[0027] According to the formulation requirements, the active ingredients, wetting and dispersing agents, stabilizers, thickeners, defoamers, preservatives, and deionized water are mixed and pre-dispersed under high shear. Then, the mixture is added to a sand mill and ground until D90 is less than 5 μm to obtain the suspension. The following examples all follow the above preparation process.

[0028] Example 1

[0029] 18% tebufenozide·Jinggangmycin A suspension

[0030] The formulation, by weight percentage, consists of: 6% tebufenozide, 12% jinggangmycin A, 4% Dispersogen ACP, 2% YUS-D3020, 0.5% meglumine, 4% propylene glycol, 0.12% xanthan gum, 1.5% magnesium aluminum silicate, 0.2% EQ preservative, and 0.2% SAG-1522, with water to bring the total to 100%. The above materials are added to a mixing vessel, stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0031] Comparative Example 1

[0032] 18% tebufenozide·Jinggangmycin A suspension

[0033] The formulation requirements, by weight percentage, are: 6% acetamiprid, 12% jinggangmycin A, 4% Dispersogen ACP 120%, 2% YUS-D3020, 4% propylene glycol, 0.12% xanthan gum, 1.5% magnesium aluminum silicate, 0.2% preservative EQ, 0.2% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0034] Example 2

[0035] 18% tebufenozide·Jinggangmycin A suspension

[0036] The formulation requirements, by weight percentage, are: 6% tebuconazole, 12% jinggangmycin A, and Atlox... TMThe following ingredients are added to a mixing tank: 49134%, Ethylan NS-500LQ 2%, SK-20TX 1%, meglumine 0.5%, propylene glycol 4%, xanthan gum 0.15%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0037] Comparative Example 2

[0038] 18% tebufenozide·Jinggangmycin A suspension

[0039] The formulation requirements, by weight percentage, are: 6% tebufenozide, 12% jinggangmycin A, and Atlox... TM The following ingredients are added to a mixing tank: 49134%, Ethylan NS-500LQ 2%, SK-20TX 1%, propylene glycol 4%, xanthan gum 0.15%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0040] Example 3

[0041] 18% tebufenozide·Jinggangmycin A suspension

[0042] The formulation, by weight percentage, consists of: 6% oxadiazon, 12% jinggangmycin A, 4% Dispersogen ACP, 2% YUS-D3020, 1.0% Morwet D425, 0.5% meglumine, 4% propylene glycol, 0.12% xanthan gum, 1.5% magnesium aluminum silicate, 0.2% preservative EQ, and 0.2% SAG-1522, with water to bring the total to 100%. The above materials are added to a mixing vessel, stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0043] Comparative Example 3

[0044] 18% tebufenozide·Jinggangmycin A suspension

[0045] The formulation requirements, by weight percentage, are as follows: 6% tebufenozide, 12% jinggangmycin A, 4% Dispersogen ACP, 2% YUS-D3020, 0.5% Morwet D425, 4% propylene glycol, 0.12% xanthan gum, 1.5% magnesium aluminum silicate, 0.2% preservative EQ, 0.2% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, mix using a high-shear disperser, and then mill using a sand mill to obtain the suspension.

[0046] Example 4

[0047] 18% tebufenozide·Jinggangmycin A suspension

[0048] The formulation requirements, by weight percentage, are: 6% tebuconazole, 12% jinggangmycin A, and Atlox... TM The following ingredients are added to a mixing tank: 49134%, Ethylan NS-500LQ 2%, SK-20TX 1%, Morwet D425 3%, meglumine 1.0%, ethylene glycol 4%, xanthan gum 0.15%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0049] Comparative Example 4

[0050] 18% tebufenozide·Jinggangmycin A suspension

[0051] The formulation requirements, by weight percentage, are: 6% tebuconazole, 12% jinggangmycin A, and Atlox... TM The following ingredients are added to a mixing tank: 49134%, Ethylan NS-500LQ 2%, SK-20TX 1%, Morwet D425 1%, ethylene glycol 4%, xanthan gum 0.15%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0052] Example 5

[0053] 15% tebufenozide·Jinggangmycin A suspension

[0054] The formulation requirements, by weight percentage, are: 5% tebuconazole, 10% jinggangmycin A, The following ingredients are added to a mixing tank: 27004%, Genapol PF40 2%, meglumine 0.5%, ethylene glycol 4%, xanthan gum 0.18%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0055] Comparative Example 5

[0056] 15% tebufenozide·Jinggangmycin A suspension

[0057] The formulation requirements, by weight percentage, are: 5% tebuconazole, 10% jinggangmycin A, The following ingredients are added to a mixing tank: 27004%, Genapol PF40 2%, ethylene glycol 4%, xanthan gum 0.18%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0058] Example 6

[0059] 15% tebufenozide·Jinggangmycin A suspension

[0060] The formulation requirements, by weight percentage, are as follows: 5% tebuconazole, 10% jinggangmycin A, 4% D305, 2% D800, 1.0% meglumine, 4% ethylene glycol, 0.18% xanthan gum, 2.0% magnesium aluminum silicate, 0.2% preservative EQ, and 0.2% SAG-1522, with water to bring the total to 100%. Add the above materials to a mixing vessel, mix using a high-shear disperser, and then mill using a sand mill to obtain the suspension.

[0061] Comparative Example 6

[0062] 15% tebufenozide·Jinggangmycin A suspension

[0063] The formulation requirements, by weight percentage, are: 5% oxamycin, 10% jinggangmycin A, 4% D305, 2% D800, 4% ethylene glycol, 0.18% xanthan gum, 2.0% magnesium aluminum silicate, 0.2% preservative EQ, 0.2% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, mix using a high-shear disperser, and then mill using a sand mill to obtain the suspension.

[0064] Example 7

[0065] 15% tebufenozide·Jinggangmycin A suspension

[0066] The formulation requirements, by weight percentage, are: 5% tebuconazole, 10% jinggangmycin A, The following ingredients are added to a mixing tank: 27004%, Genapol PF40 2%, Morwet D425 2.0%, meglumine 2.0%, ethylene glycol 4%, xanthan gum 0.18%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0067] Comparative Example 7

[0068] 15% tebufenozide·Jinggangmycin A suspension

[0069] The formulation requirements, by weight percentage, are: 5% tebuconazole, 10% jinggangmycin A, The following ingredients are added to a mixing tank: 27004%, Genapol PF40 2%, Morwet D425 2.0%, ethylene glycol 4%, xanthan gum 0.18%, magnesium aluminum silicate 2.0%, preservative EQ 0.2%, SAG-1522 0.2%, and water to 100%. The mixture is stirred and mixed using a high-shear disperser, and then milled using a sand mill to obtain the suspension.

[0070] Example 8

[0071] 15% tebufenozide·Jinggangmycin A suspension

[0072] The formulation requirements, by weight percentage, are as follows: 5% tebuconazole, 10% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 2.0% meglumine, 4% ethylene glycol, 0.18% xanthan gum, 2.0% magnesium aluminum silicate, 0.2% preservative EQ, 0.2% SAG-1522, and water to 100%. Add the above materials to a mixing tank, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0073] Comparative Example 8

[0074] 15% tebufenozide·Jinggangmycin A suspension

[0075] The formulation requirements, by weight percentage, are as follows: 5% tebuconazole, 10% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 4% ethylene glycol, 0.18% xanthan gum, 2.0% magnesium aluminum silicate, 0.2% preservative EQ, 0.2% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0076] Example 9

[0077] 21% tebufenozide·Jinggangmycin A suspension

[0078] The formulation requirements, by weight percentage, are as follows: 7% oxadiazon, 14% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 1.0% meglumine, 4% ethylene glycol, 0.10% xanthan gum, 1.2% magnesium aluminum silicate, 0.2% preservative EQ, 0.4% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0079] Comparative Example 9

[0080] 21% tebufenozide·Jinggangmycin A suspension

[0081] The formulation requirements, by weight percentage, are as follows: 7% oxadiazon, 14% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 4% ethylene glycol, 0.10% xanthan gum, 1.2% magnesium aluminum silicate, 0.2% preservative EQ, and 0.4% SAG-1522, with water to bring the total to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0082] Example 10

[0083] 30% tebufenozide·Jinggangmycin A suspension

[0084] The formulation requirements, by weight percentage, are as follows: 10% tebuconazole, 20% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 1.0% meglumine, 4% ethylene glycol, 0.08% xanthan gum, 1.0% magnesium aluminum silicate, 0.2% preservative EQ, 0.4% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0085] Comparative Example 10

[0086] 30% tebufenozide·Jinggangmycin A suspension

[0087] The formulation requirements, by weight percentage, are as follows: 10% tebuconazole, 20% jinggangmycin A, 4% D305, 2% D800, 2.0% Morwet D425, 4% ethylene glycol, 0.08% xanthan gum, 1.0% magnesium aluminum silicate, 0.2% preservative EQ, 0.4% SAG-1522, and water to 100%. Add the above materials to a mixing vessel, stir and mix using a high-shear disperser, and then grind using a sand mill to obtain the suspension.

[0088] thermal storage stability test

[0089] Thermal storage performance tests were conducted on the oxadiazon·jinggangmycin A suspensions prepared in Examples 1-10 and Comparative Examples 1-10.

[0090] Test method for thermal storage performance: The acetamiprid·jinggangmycin A suspensions prepared in Examples 1-10 and Comparative Examples 1-10 were tested according to method 2.1 "Liquid Preparations" in GB / T 19136-2003. After storage at (54±2)℃ for 14 days, the particle size and acetamiprid suspension rate of the samples were measured. The test results are shown in Table 1.

[0091] Methods for determining suspension rate:

[0092] Weigh 1 g of the sample (accurate to 0.0002 g), and proceed according to CIPAC MT184. Transfer the remaining 25 mL of suspension and precipitate to a 100 mL volumetric flask, dilute to volume with methanol, and dissolve the sample by ultrasonic vibration. Filter the solution and determine the mass of the active ingredient, then calculate the suspension rate. A suspension rate ≥90% for each component is generally considered acceptable.

[0093] Table 1 Results of thermal storage stability testing

[0094]

[0095] As shown in Table 1, in Comparative Examples 1-10, when preparing formulations by combining tebuconazole and jinggangmycin, the difference in solubility between the two substances caused them to interfere with each other during dilution in water, resulting in incompatibility and uneven particle size. This led to a low suspension rate of tebuconazole, especially after heat storage, where the particle size of the samples increased significantly, and the suspension rate of tebuconazole was found to be below 90%. In Examples 1, 2, 5, and 6, the stabilizer meglumine was added, which showed that it could inhibit the increase in particle size of the heat-stored samples to a certain extent, and the suspension rate of tebuconazole was significantly improved after heat storage. In the examples (Examples 3, 4, 7, 8, 9 and 10), the sodium salt adjuvant Morwet D425 of alkyl naphthalene sulfonic acid condensate was combined with meglumine, which significantly improved the suspension rate of acetamiprid in the heat storage samples. This problem can be effectively solved, which can effectively solve the compatibility problem of pesticide formulation mixing and dilution application, and bring practical application significance.

[0096] Example 11 Indoor efficacy test of bactericidal activity

[0097] To verify the effect of adding stabilizer meglumine and other adjuvants to tebufenozide·jinggangmycin A suspension on efficacy, the following bioactivity test was conducted: The control effects of Example 7, Comparative Example 7, Example 8, and Comparative Example 8 on rice sheath blight were compared in the test.

[0098] The test concentrations of the tested agents were 100, 50, 25, 12.5, and 6.25 mg / mL. A control group was prepared by mixing two original pesticides. Each treatment was replicated twice. Uniformly growing rice seedlings with the same leaf age were selected and treated with the above-mentioned pesticide concentrations using a crop sprayer. After spraying, the seedlings were dried in a fume hood. 24 hours later, they were inoculated with rice sheath blight pathogens using the inoculation block method, with three 0.25 cm blocks inoculated per pot. 2 Rice sheath blight pathogen blocks were inoculated at the base of rice plants. After inoculation (temperature: 28℃ during the day, 25℃ at night), the plants were cultured for 7 days, and the results were investigated. Investigation method: Six rice seedlings with inoculated blocks were examined in each pot. The control effect was calculated using the disease index, and the EC90 value (high inhibitory concentration) was calculated. The test results are shown in Table 2.

[0099] Grading standards for rice sheath blight: Grade 0: No disease in the whole plant; Grade 1: Disease occurs on the fourth leaf and all leaf sheaths and leaves below it (with the flag leaf as the first leaf); Grade 3: Disease occurs on the third leaf and all leaf sheaths and leaves below it; Grade 5: Disease occurs on the second leaf and all leaf sheaths and leaves below it; Grade 7: Disease occurs on the flag leaf and all leaf sheaths and leaves below it; Grade 9: Disease occurs in the whole plant, leading to premature death.

[0100] Methods for calculating drug efficacy:

[0101] Disease index (%) = ∑(Number of diseased plants at each level × Relative disease level value) ÷ (Total number of plants surveyed × Highest representative value) × 100

[0102] Control efficacy (%) = 1 - (Disease index in the treated area after application × Disease index in the control area before application) ÷ (Disease index in the treated area before application × Disease index in the control area after application) × 100

[0103] Table 2 Results of pot experiment on the inhibition of rice sheath blight

[0104]

[0105] Pot experiments on the control of rice sheath blight showed that the stabilizer provided by this invention had no negative impact on the efficacy of the product. In practical applications, the prepared suspension exhibited excellent suspension rates, ensuring the normal exertion of the active ingredients.

Claims

1. A suspension containing tebufenozide and jinggangmycin A, comprising an active ingredient, a stabilizer, an adjuvant, and water, characterized in that: The active ingredient accounts for 1-70% of the formulation weight, and the stabilizer accounts for 0.1-10% of the formulation weight; wherein, the active ingredient is tebufenozide and jinggangmycin A, and the stabilizer is meglumine (N-methyl-D-glucosamine).

2. The suspension containing tebufenozide and jinggangmycin A according to claim 1, characterized in that: The amount of acetamiprid is 1-40% by weight of the formulation, and the amount of jinggangmycin A is 1-40% by weight of the formulation.

3. The suspension containing tebufenozide and jinggangmycin A according to claim 2, characterized in that: The amount of acetamiprid is 1-10% by weight of the formulation, and the amount of jinggangmycin A is 1-20% by weight of the formulation.

4. The suspension containing tebufenozide and jinggangmycin A according to claim 1, characterized in that: The adjuvant is one or more of the following: wetting and dispersing agent, thickener, defoamer, and antifreeze agent. The components account for 1-20% of the weight of the formulation, 0-2% of the wetting and dispersing agent, 0-2% of the thickener, 0-2% of the defoamer, 0-2% of the preservative, and 0-10% of the antifreeze agent.

5. The suspension containing tebufenozide and jinggangmycin A according to claim 4, characterized in that: The wetting and dispersing agent is selected from one or more of the following: lignin sulfonates, sodium salts of alkyl naphthalene sulfonate condensates, alkyl naphthalene sulfonate formaldehyde condensates, polycarboxylates, EO / PO block copolymers, phosphate anions, sulfate anions, polyacrylate copolymers, fatty alcohol polyoxyethylene ethers, sorbitan fatty acid ester polyoxyethylene ethers, and methacrylic acid grafted comb copolymers.

6. The suspension containing tebufenozide and jinggangmycin A according to claim 5, characterized in that: The wetting and dispersing agent is a sodium salt of alkyl naphthalene sulfonic acid condensate and a mixture of one or more of the following substances: lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, polycarboxylate salts, EO / PO block copolymers, phosphate ester anions, sulfate anions, polyacrylate copolymers, fatty alcohol polyoxyethylene ethers, sorbitan fatty acid ester polyoxyethylene ethers, and methacrylic acid grafted comb copolymers.

7. The suspension containing tebufenozide and jinggangmycin A according to any one of claims 1-6, characterized in that: The mass ratio of the stabilizer meglumine (N-methyl-D-glucosamine) to the sodium salt of the alkyl naphthalene sulfonic acid condensate in the wetting and dispersing agent is 1:1 to 1:

10.

8. The suspension containing tebufenozide and jinggangmycin A according to claim 4, characterized in that: The thickener is selected from one or more of xanthan gum, bentonite, gelatin, gum arabic, polyvinyl acetate, polyvinylpyrrolidone, magnesium aluminum silicate, polyvinyl alcohol, polyethylene glycol, phenolic resin, shellac, and hydroxymethyl cellulose. The defoamer is selected from one or more of SAG-1522, SAG-1572, silicones, C8-10 fatty alcohols, phosphate esters, C10-20 saturated fatty acids (such as decanoic acid) and amides; The preservative is selected from sodium benzoate, Kathon (2-methyl-4-isothiazolin-3-one and (B)5-chloro-2-methyl-4-isothiazolin-3-one), potassium sorbate, sodium dehydroacetate or one or more of the following; The antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea.

9. The application of the suspension containing tebufenozide and jinggangmycin A as described in claim 1, characterized in that: The application of the suspension in agricultural fungicides.

10. The application of the suspension containing tebufenozide and jinggangmycin A according to claim 9, characterized in that: The formulation is applied at a concentration of 10-500 grams of active ingredient per hectare to the pathogens to be controlled or their growth medium. It can be used to control one or more plant diseases, including rice sheath blight, rice blast, wheat powdery mildew, wheat rust, soybean rust, soybean gray spot, cotton damping-off, cotton anthracnose, cucumber powdery mildew, cucumber downy mildew, grape downy mildew, apple leaf spot, apple powdery mildew, banana leaf spot, tomato early blight, strawberry powdery mildew, and rapeseed sclerotinia stem rot.

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