A revitalizing and strengthening composition suitable for sensitive skin, its preparation method and application

Abstract

This application discloses a revitalizing and strengthening composition suitable for sensitive skin, its preparation method, and its application, relating to the field of cosmetic technology. This application provides a revitalizing and strengthening composition comprising the following components in parts by weight: 1-2 parts of mulberry root extract, 0.1-2 parts of ceramide, 0.3-0.8 parts of polyglycerol-6 distearate, 0.3-0.8 parts of sorbitan olive oil ester, and 2.5-4 parts of hydrogenated lecithin; wherein the ceramide is a mixture of ceramide EOP and ceramide NP; the revitalizing and strengthening composition is a nanoemulsion with a layered liquid crystal structure, the nanoemulsion comprising an oil phase and an aqueous phase, the oil phase comprising ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin, and the aqueous phase comprising mulberry root extract. This application provides a revitalizing and strengthening composition capable of mimicking beneficial light signals, activating and regulating skin opsin pathways, thereby specifically improving skin problems caused by prolonged exposure to indoor environments.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and in particular to a revitalizing and strengthening composition suitable for sensitive skin, its preparation method, and its application. Background Technology

[0002] Urban indoor workers are often exposed to specific environments characterized by a lack of natural light, constant artificial lighting, and dry, circulating air. Their skin faces a series of unique "indoor environment-related" problems, primarily including: decreased self-repair capabilities due to disrupted skin circadian rhythms, "office-sensitive skin" caused by weakened barrier function, dull and lackluster skin tone, and insufficient defense against sudden outdoor sunlight. These problems are more pronounced in people with sensitive skin, whose already fragile barrier and low tolerance threshold exacerbate these issues, making them more prone to a vicious cycle of repeated damage. The common physiological root of these problems lies in the fact that the skin, as the largest peripheral photoreceptor organ in the human body, suffers from a chronic "signal starvation" or "rhythm disorder" in the opsin photoreceptor system expressed on the surface of its cells (keratinocytes, melanocytes, etc.). Opsins, especially opsin 4 which senses blue light and opsin 5 which is sensitive to UVB, are key molecular switches for the skin to perceive ambient light, calibrate its internal circadian rhythm, and activate daytime defense modes (such as antioxidant preparation and DNA repair preparation). A prolonged lack of effective natural light signal input leads to dysfunction of this sophisticated system.

[0003] Currently, skincare products targeting urban dwellers primarily focus on "moisturizing," "antioxidant," or "blue light protection," but their technological solutions have inherent flaws. Moisturizing and barrier repair products can only passively replenish lipids and moisture, failing to address the fundamental problem of reduced active repair capabilities due to disrupted skin circadian rhythms. Traditional antioxidant products (such as Vitamin C and Vitamin E) provide exogenous antioxidant protection but cannot "train" or activate the skin's own endogenous antioxidant defense system, thus only treating the symptoms, not the root cause. Blue light protection products mainly rely on physical or chemical sunscreens to block or absorb blue light from electronic screens, a purely "defense" strategy. However, it completely ignores the fact that specific wavelengths and intensities of blue light (especially blue light in natural morning light) are "beneficial signals" necessary for activating skin opsins and calibrating the circadian rhythm. Indiscriminately blocking all blue light may further exacerbate the skin's "light signal hunger."

[0004] Existing technologies have failed to offer solutions from the perspective of "actively regulating the function of the skin's photoreceptor system and restoring its autonomous rhythm and intelligent defense capabilities," and cannot fundamentally improve the systemic skin function degeneration caused by prolonged indoor living, especially the core problems faced by sensitive skin such as irritation, recurring redness, and weakened barrier function. Therefore, a revitalizing and strengthening composition that can simulate beneficial light signals, activate and regulate the skin's opsin pathways, and thus specifically improve skin problems caused by prolonged indoor living has broad application prospects. Summary of the Invention

[0005] Based on this, the purpose of this application is to overcome the shortcomings of the prior art and provide a revitalizing and strengthening composition, preparation method and application thereof, which can simulate beneficial light signals, activate and regulate the skin opsin pathway, thereby specifically improving skin problems caused by long-term indoor environment, especially suitable for use on sensitive skin with weak barrier function and low tolerance threshold.

[0006] To achieve the above objectives, the technical solution adopted in this application is: a revitalizing and strengthening composition, comprising the following components in parts by weight: Mulberry root extract 1-2 parts, ceramide 0.1-2 parts, polyglycerol-6 distearate 0.3-0.8 parts, sorbitan olive oil ester 0.3-0.8 parts, hydrogenated lecithin 2.5-4 parts; The ceramide is a mixture of ceramide EOP and ceramide NP; The revitalizing and strengthening composition is a nanoemulsion with a layered liquid crystal structure. The nanoemulsion includes an oil phase and an aqueous phase. The oil phase includes ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin. The aqueous phase includes mulberry root extract.

[0007] In the revitalizing and strengthening composition of this application, mulberry root extract, on the one hand, through its active ingredients such as morin, morin bark extract, and other flavonoid derivatives, mimics beneficial light signals, gently activating the stress adaptation and defense pathways of skin cells, inducing cells to produce responses similar to those after receiving light stimulation, and initiating the preparative expression of endogenous antioxidant systems (such as SOD). On the other hand, it regulates rhythm-related genes, awakening the skin's basic defense and vitality at the cellular level, providing chemical signals to correct circadian rhythm inaccuracies and insufficient defense preparation. In the revitalizing and strengthening composition of this application, the combination of ceramide EOP and NP can rapidly repair the skin barrier, reduce signal interference from external stimuli, and provide a stable skin microenvironment for the activation of opsin pathways. Physiologically, ceramide EOP mainly covalently binds to the keratinized capsule on the surface of keratinocytes, acting as an "anchor" between the lipid bilayer and cells, and is a key "anchor point" for the integrity of the barrier structure; while ceramide NP mainly fills the interlaminar spaces, providing hydration and flexibility. Both ceramides EOP and NP are skin homologous structures with high compatibility. They can synergistically promote keratinocyte differentiation and lipid synthesis, indirectly supporting the normal expression and function of opsin-related pathways. The synergistic effect of EOP and NP not only immediately fills gaps in the physical barrier but also transmits the correct differentiation and assembly signals to keratinocytes, promoting the autonomous and systematic reconstruction of barrier function and directly combating barrier fragility. In actual experiments, the inventors of this application discovered that not all ceramides or combinations thereof can effectively support the photorepair effect simulated by mulberry root extract. The combination of ceramides EOP and NP enables immediate repair and long-term strengthening of the skin barrier, providing the material basis for the successful execution of repair behaviors activated by cells after receiving light signals.

[0008] In the revitalizing and strengthening composition of this application, polyglycerol-6 distearate and sorbitan olive oil ester, together with hydrogenated lecithin and ceramide, construct a highly ordered layered liquid crystal nanoemulsion. Due to their specific hydrophilic-lipophilic balance (HLB) value and molecular chain structure, these two components can effectively regulate the viscoelasticity and packing density of the interfacial film, promoting the formation of a multilayered arrangement of the oil and aqueous phases at the microscopic level. This layered liquid crystal structure not only significantly enhances the physical stability of the nanoemulsion system, preventing delamination or aggregation during storage, but more importantly, its multilayered film structure can tightly encapsulate mulberry root extract and ceramide, forming a physical barrier to prevent water and oxygen contact, effectively preventing the oxidation or hydrolytic inactivation of active ingredients. Furthermore, its unique interlayer reservoir effect enables the gradient slow release of active ingredients, reducing the risk of instantaneous irritation while guiding the effective ingredients to penetrate orderly into the deep layers of the epidermis, thereby maximizing the revitalizing and repairing effects while ensuring system stability.

[0009] This application utilizes a nanoemulsion with a layered liquid crystal structure, synergistically prepared from mulberry root extract, ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin, to form a dynamic micro-repair system on the skin. The mulberry root extract internally awakens cellular function, enhancing its synthetic and repair capabilities. Meanwhile, the ceramide and other components in the nanoemulsion provide optimal repair raw materials for the awakened cells. The two are closely linked in time and space, forming a functional closed loop of signal activation, raw material supply, and structural reconstruction, thereby systematically addressing the comprehensive decline in skin function caused by insufficient light signal input. This application also provides a revitalizing and strengthening composition that can mimic beneficial light signals, activate and regulate the skin's opsin pathway, thereby specifically improving skin problems caused by prolonged exposure to indoor environments. This composition further enhances skin tolerance, repairs the skin barrier, improves skin tone, and enhances self-repair capabilities.

[0010] In some embodiments, the revitalizing and strengthening composition comprises the following components in parts by weight: 1.2-1.8 parts of mulberry root extract, 0.5-1.5 parts of ceramide, 0.4-0.6 parts of polyglycerol-6 distearate, 0.4-0.6 parts of sorbitan olive oil ester, and 2.8-3.5 parts of hydrogenated lecithin.

[0011] In actual experiments, the inventors of this application discovered that when the components in the revitalizing and strengthening composition are within the above-mentioned range, the effect of simulating beneficial light signals, activating and regulating the skin's opsin pathway is better, further improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability.

[0012] In some embodiments, the mulberry root extract is present in a weight range of 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, or 2 parts, or any two of these values; the ceramide is present in a weight range of 0.1 parts, 0.5 parts, 0.8 parts, 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, or 2 parts, or any two of these values. The polyglycerol-6 distearate is present in a weight range of 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, and 0.8 parts, or any two of these values; the sorbitan olive oil ester is present in a weight range of 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, and 0.8 parts, or any two of these values; and the hydrogenated lecithin is present in a weight range of 2.5 parts, 2.8 parts, 3.0 parts, 3.5 parts, 3.8 parts, and 4.0 parts, or any two of these values.

[0013] In some embodiments, the weight ratio of ceramide NP to ceramide EOP is (0.5-5):1.

[0014] In some embodiments, the weight ratio of ceramide NP to ceramide EOP can be one or any two of the following: 0.5:1, 0.8:1, 1.0:1, 1.5:1, 2.0:1, 2.5:1, 3.0:1, 3.5:1, 4.0:1, 4.5:1, 5.0:1.

[0015] In some embodiments, the weight ratio of ceramide NP to ceramide EOP is (1-2):1.

[0016] In actual experiments, the inventors of this application discovered that when ceramide NP and ceramide EOP are combined in a specific ratio, they can better achieve immediate repair and long-term strengthening, improve overall density, and provide the most suitable repair raw materials while mulberry root extract awakens the cell repair process. This better simulates beneficial light signals, activates and regulates the skin opsin pathway, further improves skin tolerance, repairs the skin barrier, improves skin tone, and enhances self-repair ability.

[0017] In some embodiments, the preparation method of the mulberry root extract includes the following steps: The mulberry root is crushed and sieved to obtain mulberry root powder; The mulberry root powder was mixed with an organic solvent and subjected to ultra-high pressure and low temperature extraction. The filtrate was collected after extraction. The extraction pressure was 300-500 MPa and the extraction temperature was 25-35℃. The filtrate was concentrated to a paste and freeze-dried to constant weight to obtain the mulberry root extract.

[0018] In some embodiments, the extraction pressure is a range of one or any two of 300MPa, 320MPa, 350MPa, 380MPa, 400MPa, 420MPa, 450MPa, 480MPa, and 500MPa; and the extraction temperature is a range of one or any two of 25°C, 28°C, 30°C, 32°C, and 35°C.

[0019] This application does not specify a particular method for preparing mulberry root extract, such as ultrasonic extraction, reflux extraction, or microwave extraction. All methods that can yield mulberry root extract are suitable for this application. The applicant's research has found that mulberry root extracts prepared by different extraction methods exhibit differences in performance, and the composition and ratio of active substances obtained by different extraction methods differ. This application further provides a method for preparing mulberry root extract through ultra-high pressure and low temperature extraction. This method avoids thermal damage and fully preserves active substances in mulberry root that have the ability to simulate light signals and activate the opsin 4 (OPN4) and opsin 5 (OPN5) pathways in skin cells, such as morin, morin bark extract, and other flavonoid derivatives. The final extract obtained is significantly superior to products obtained by traditional methods in terms of light signal simulation performance.

[0020] In some embodiments, the sieve mesh size is 20-50 mesh.

[0021] In some implementations, the extraction time is 5-15 minutes.

[0022] In some embodiments, the organic solvent is an ethanol solution with a volume fraction of 40-60%.

[0023] In some embodiments, the mass-to-volume ratio of the mulberry root powder to the organic solvent is 1 g: (10-15) mL.

[0024] In some embodiments, the ultra-high pressure low temperature extraction is performed 1-3 times.

[0025] In a second aspect of this application, a method for preparing the revitalizing and strengthening composition is provided, comprising the following steps: S1. The ceramide, polyglycerol-6 distearate, sorbitan olive oil ester and hydrogenated lecithin are heated and mixed evenly to obtain the oil phase; S2. Mix the mulberry root extract with heated water until homogeneous to obtain an aqueous phase; S3. The oil phase is added dropwise to the aqueous phase, and shearing is performed to form a nanoemulsion; S4. After homogenizing the nanoemulsion, cool it, adjust the pH value, and filter to obtain the revitalizing and toughening composition.

[0026] In some embodiments, in step S1, the temperature is heated to 60-80°C, the mixing speed is 300-1000 rpm, and the mixing time is 10-30 min.

[0027] In some embodiments, in step S2, the water is heated to 60-80°C, the mixing speed is 300-1000 rpm, and the mixing time is 2-5 min.

[0028] In some embodiments, in step S3, the shearing speed is 8000-20000 rpm, the shearing time is 5-15 min, and the shearing temperature is 60-80℃.

[0029] In some embodiments, in step S4, the homogenization pressure is 1000-1500 bar, the homogenization is performed 2-4 times, the mixture is cooled to room temperature, and the pH value is adjusted to 5.0-7.0.

[0030] This application prepares a nanoemulsion with a layered liquid crystal structure from mulberry root extract, ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin. This layered liquid crystal structure possesses dense multilayer film properties, effectively encapsulating morin, morin bark extract, other flavonoid derivatives, and ceramide in the mulberry root extract. By physically blocking water and oxygen contact, it prevents oxidative degradation or hydrolytic inactivation of active ingredients during storage and use, ensuring the bioavailability of key functional molecules. This carrier not only reduces transdermal transport resistance, guiding the mulberry root extract to precisely penetrate target cells to activate the opsin pathway, but also assists in the orderly embedding of ceramide into damaged barrier gaps. Its unique multilayer reservoir effect enables gradient sustained release of active ingredients, avoiding the potential irritation to sensitive skin from instantaneous high-concentration release. It ensures the synergistic and continuous effect of "photosignal simulation" and "barrier physical repair" in time and space, thereby maximizing the revitalizing and strengthening efficacy.

[0031] In a third aspect of this application, the application is provided for the use of the described revitalizing and strengthening composition in the preparation of cosmetics.

[0032] The cosmetics provided by this invention have significant effects on improving skin problems such as dullness, roughness, fragile skin barrier, and increased sensitivity in people who work indoors for long periods of time and are not exposed to sunlight, especially those with sensitive skin.

[0033] In a fourth aspect, this application provides a cosmetic product comprising the revitalizing and strengthening composition described above; wherein the revitalizing and strengthening composition is present in the cosmetic product at a weight percentage of 2-5%.

[0034] In some embodiments, the cosmetic includes one of the following: toner, lotion, cream, mask, and serum.

[0035] In some embodiments, the cosmetic is a face cream comprising the following components by weight percentage: 2%-5% revitalizing and strengthening composition, 0.05%-1% thickener, 1%-5% emulsifier, 1%-5% oil, 0.5%-10% moisturizer, 0.1%-1% antioxidant, and the balance being water.

[0036] Preferably, the face cream comprises at least one of the following: (a)-(f) (a) The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum; (b) The emulsifier comprises at least one of C14-22 alcohol, C12-20 alkyl glucoside, and sucrose stearate; (c) The oil comprises at least one of caprylic / capric triglyceride, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethyl silsesquioxane, and pentaerythritol distearate; (d) The humectant comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, 1,3-propanediol, 1,2-hexanediol, 1,2-pentanediol, glycerin, and D-panthenol; (e) The antioxidant comprises p-hydroxyacetophenone.

[0037] Compared to existing technologies, the beneficial effects of this application are as follows: This application utilizes a nanoemulsion with a layered liquid crystal structure, synergistically prepared from mulberry root extract, ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin. This nanoemulsion forms a dynamic micro-repair system on the skin. The mulberry root extract awakens cell function from within, enhancing its synthesis and repair capabilities; while the ceramide and other components contained in the nanoemulsion provide optimal repair raw materials for the awakened cells. The two are closely integrated in time and space, forming a functional closed loop of signal activation-raw material supply-structural reconstruction, thereby systematically solving the comprehensive decline in skin function caused by insufficient light signal input. This application provides a revitalizing and strengthening composition that can simulate beneficial light signals, activate and regulate the skin's opsin pathway, thereby specifically improving skin problems caused by prolonged exposure to indoor environments. This composition further enhances skin tolerance, repairs the skin barrier, improves skin tone, and enhances self-repair capabilities. Attached Figure Description

[0038] Figure 1 This is a microscopic morphology image of the nanoemulsion with a layered liquid crystal structure prepared in Example 1 of the present invention.

[0039] Figure 2 These are cell proliferation test images for Example 1 and Comparative Example 1; Figure 3 Example 1 is a diagram illustrating the human body's functions. Detailed Implementation

[0040] To better illustrate the purpose, technical solution, and advantages of this application, the following description, in conjunction with the accompanying drawings and specific embodiments, will further explain this application. The purpose is to provide a detailed understanding of the content of this application, not to limit it. All other embodiments obtained by those skilled in the art without inventive effort are within the protection scope of this application. Unless otherwise specified, the experimental reagents and instruments designed in the embodiments and comparative examples of this application are commonly used reagents and instruments, all of which are commercially available. Unless otherwise specified, the experimental methods used in the embodiments and comparative examples are conventional methods; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0041] The raw materials used in this application are described below, but are not limited to the following: Ceramide NP was purchased from Shenzhen Dickman Biotechnology Co., Ltd., and its trade name is ceramide 3. Ceramide EOP was purchased from Shenzhen Dickman Biotechnology Co., Ltd., under the trade name EOP Cer. Polyglycerol-6 distearate and sorbitan oleate were purchased from Seppic, France; Hydrogenated lecithin was purchased from Nikko Chemical, under the trade name NIKKOL LECINOL S-10; Mulberry root extract-1: self-made, prepared as follows: Mulberry root was pulverized and passed through a 40-mesh sieve to obtain mulberry root powder; the mulberry root powder was mixed with a 60% (v / v) ethanol solution, the mass-to-volume ratio of the mulberry root powder to the organic solvent was 1 g: 15 mL, and ultra-high pressure low temperature extraction was performed at a pressure of 450 MPa and a temperature of 32 °C for 15 min. After extraction, the filtrate was collected, and the extraction was performed twice; the filtrate was concentrated under reduced pressure at 40 °C to a paste, and freeze-dried to constant weight to obtain the mulberry root extract.

[0042] Mulberry root extract-2: prepared in-house. The preparation method is the same as that of mulberry root extract-1, except that the volume fraction of the ethanol solution is different. A 45% volume fraction ethanol solution is selected. The other components, weight parts and preparation methods are exactly the same.

[0043] Mulberry root extract-3: prepared in-house. The preparation method is the same as that of mulberry root extract-1, except that the mass-volume ratio of mulberry root powder to organic solvent is different. The mass-volume ratio of mulberry root powder to organic solvent is 1g:10mL. All other components, weight parts and preparation methods are exactly the same.

[0044] Mulberry root extract-4: self-made. The preparation method is the same as that of mulberry root extract-1, except that the pressure and temperature of ultra-high pressure low temperature extraction are different. The pressure of ultra-high pressure low temperature extraction is 350 MPa and the temperature is 28℃. The other components, weight parts and preparation methods are exactly the same.

[0045] Mulberry root extract-5: self-made. The preparation method is the same as that of mulberry root extract-1, except that the extraction temperature is different. The extraction temperature is 45℃. All other components, weight parts and preparation methods are exactly the same.

[0046] Mulberry root extract-6: self-made. The preparation method is the same as that of mulberry root extract-1, except that the extraction pressure is different. The extraction pressure is 200 MPa. The other components, weight parts and preparation methods are exactly the same.

[0047] Mulberry root extract-7: Prepared in-house, the preparation method is heating and reflux extraction. Mulberry roots are pulverized and passed through a 40-mesh sieve to obtain mulberry root powder. The mulberry root powder is mixed with a 60% (v / v) ethanol solution. The mass-to-volume ratio of the mulberry root powder to the organic solvent is 1 g: 15 mL. After soaking for 30 min, the mixture is heated and refluxed at 50 °C for 2 h. After extraction, the filtrate is collected by filtration. The extraction is repeated twice, and the filtrates are combined. The filtrate is concentrated under reduced pressure to a paste, and then freeze-dried to constant weight to obtain the mulberry root extract.

[0048] Mulberry root extract-8: self-made, prepared by ultrasonic extraction. Mulberry root was pulverized and passed through a 40-mesh sieve to obtain mulberry root powder. The mulberry root powder was extracted with 40 times the volume of 60% ethanol solution. The ultrasonic extraction was repeated twice and the filtrate was collected. The ultrasonic temperature was 50℃, the power was 200W, and the extraction time was 15min. The extract was concentrated under reduced pressure to a paste and freeze-dried to constant weight to obtain the mulberry root extract.

[0049] Mulberry leaf extract: purchased from Fufeng Sinote Biotechnology Co., Ltd., trade name: Mulberry Leaf Extract; Ceramide AP: Purchased from Shenzhen Dickman Biotechnology Co., Ltd., trade name: Ceramide 6; Ceramide EOS: Purchased from Shenzhen Dickman Biotechnology Co., Ltd. Examples and Comparative Examples The present invention provides a revitalizing and strengthening composition in the embodiments and comparative examples. The components and weight parts of the revitalizing and strengthening composition provided in the embodiments and comparative examples are shown in Tables 1-3, and the total weight parts are 100 parts. The preparation method of the revitalizing and strengthening composition is as follows: S1. The ceramide, polyglycerol-6 distearate, sorbitan olive oil ester and hydrogenated lecithin are heated to 65°C and mixed at 500 rpm for 30 min until homogeneous to obtain the oil phase. S2. Mix the mulberry root extract with deionized water heated to 65°C until homogeneous. The mixing speed is 500 rpm and the mixing time is 5 min to obtain the aqueous phase. S3. The oil phase is added dropwise to the aqueous phase, and sheared for 10 minutes at a speed of 20,000 rpm and a temperature of 65°C to form a nanoemulsion. S4. After homogenizing the nanoemulsion, cool it to room temperature, adjust the pH value to 5.0-7.0, and filter to obtain the revitalizing and toughening composition; the homogenization pressure is 1500 bar, and the homogenization is performed 3 times.

[0050] When preparing the comparative revitalizing and strengthening composition, if the relevant component is unavailable, it can be omitted; if other components are available, they can be substituted according to the preparation method described above.

[0051] Figure 1 The image shows the microscopic morphology of the nanoemulsion with a layered liquid crystal structure prepared in Example 1 of this invention. It can be seen that the nanoemulsion with the layered liquid crystal structure exhibits typical Maltese cross extinction under the microscope. This structure significantly enhances the stability of the system and achieves long-term sustained release of active ingredients. It also simulates the skin barrier to promote transdermal absorption and greatly improves the effect of active ingredients in activating the opsin pathway and the barrier repair effect.

[0052] Table 1 Table 2 Table 3 Application examples The present invention provides a face cream in application examples, comparative application examples, and blank examples, the components (mass percentage) of which are shown in Table 4; wherein, the revitalizing and strengthening compositions used in application examples 1-15 are the revitalizing and strengthening compositions prepared in examples 1-15, and the revitalizing and strengthening compositions used in comparative application examples 1-7 are the revitalizing and strengthening compositions prepared in comparative examples 1-7.

[0053] For example, the revitalizing and toughening composition used in Application Example 1 is the revitalizing and toughening composition prepared in Example 1; the revitalizing and toughening composition used in Application Example 2 is the revitalizing and toughening composition prepared in Example 2; the revitalizing and toughening composition used in Application Example 15 is the revitalizing and toughening composition prepared in Example 15; the revitalizing and toughening composition used in Comparative Application Example 1 is the revitalizing and toughening composition prepared in Comparative Example 1; and so on.

[0054] The revitalizing and strengthening composition used in Application Example 16 is the revitalizing and strengthening composition prepared in Example 1.

[0055] The method for preparing the face cream includes the following steps: (1) Mix the A phase component with water and stir, heat to 85±2℃, then homogenize at 12000rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared A component. (2) After heating the B phase component to 85±2℃, homogenize it at 12000rpm for 4 min. After homogenization, keep it warm for later use to obtain the pre-prepared B component. (3) Mix the C phase components and heat to 60±2℃ to melt them to obtain the pre-prepared C component; (4) Heat the pre-prepared component A to 80±2℃, add the pre-prepared component B at 15000 rpm and homogenize to emulsify into a homogeneous liquid. Then cool down to 60±2℃, add the pre-prepared component C at 250 rpm and stir to mix. Then cool down to 40℃, add the revitalizing and strengthening composition and continue stirring for 8 minutes. Stop stirring, discharge the product, and obtain the face cream.

[0056] The preparation methods of the face creams provided in the other application examples, comparative application examples, and blank application examples are consistent with those in application example 1. If the relevant components are not available, they can be omitted.

[0057] Table 4 Performance Test-1: Effect of the composition on the expression of opsin genes in skin keratinocytes.

[0058] Optosins expressed by keratinocytes in the skin, such as opsin 4 (OPN4) and opsin 5 (OPN5), are key receptors for sensing ambient light signals and regulating cellular circadian rhythms. The more significant the upregulation of OPN4 and OPN5 mRNA expression levels after sample treatment, the stronger the ability of the composition to mimic light signals and activate the skin's photoreceptor system and circadian rhythm pathways, thus providing a molecular biological basis for improving skin dysfunction caused by insufficient light signal input.

[0059] Test substance preparation: Dilute the composition (Examples 1-15 and Comparative Examples 1-7) with DMEM complete culture medium containing 10% fetal bovine serum to 0.5% as the final experimental concentration, and record it as the sample solution. Prepare and use immediately.

[0060] The cell line used in this invention is human immortalized keratinocyte HaCaT (Beina Biotechnology). The test conditions are: incubator temperature of 37±1℃, saturated humidity, and carbon dioxide of 5±1%.

[0061] Test method: Real-time quantitative PCR (qRT-PCR) detection, as detailed below: (1) Cell seeding and treatment: The cell suspension was seeded at 2 × 10⁶ cells per dish. 6Cells were seeded at a density of [number] cells per 60 mm cell culture dish and cultured in DMEM complete medium containing 10% fetal bovine serum for 24 hours until cell confluence reached approximately 80%. The original culture medium was discarded, and 5 mL of sample solution was added to each of the sample groups. The blank control group was replaced with an equal volume of 5 mL of complete culture medium without the sample. The culture dishes were then returned to the incubator for further culture.

[0062] (2) Cell collection and RNA extraction: 24 hours after drug treatment, discard the culture medium, wash the cells once with PBS, add 1 mL of TRIzol lysis buffer to each dish, lyse the cells thoroughly, collect the lysis buffer in RNase-free EP tubes, and store at -80℃ or immediately extract total RNA using the standard chloroform method. Use NanoDrop to determine RNA concentration and purity (A260 / A280).

[0063] (3) cDNA synthesis: Genomic DNA removal and reverse transcription were performed using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, catalog number RR047A). 1 μg of total RNA was reverse transcribed into cDNA and stored at -20℃ for later use.

[0064] (4) qRT-PCR detection: Amplification was performed using TB Green Premix Ex Taq II (TaKaRa, catalog number RR820A) on a real-time quantitative PCR instrument (e.g., Bio-Rad CFX96). The reaction volume was 20 μL, including 10 μL of TB Green Premix, 0.8 μL each of forward and reverse primers, 2 μL of cDNA template, and 6.4 μL of sterile water. The reaction program was: 95℃ pre-denaturation for 30 seconds; 95℃ denaturation for 5 seconds, 60℃ annealing / extension for 30 seconds, for a total of 40 cycles. Melting curve analysis was used to verify the amplification specificity.

[0065] The opsin 4 (OPN4) sequence is as follows: upstream: 5'-AGC TGG TCA TCG GCT TCT TC-3', downstream: 5'-AGG AGC AGG TAG AGG AGG AG-3'.

[0066] Opsin 5 (OPN5): Upstream 5'-TGC TAC ATC GTC TGC TAC CT-3', Downstream 5'-AGA GGTGAA GGT GAG GAT GA-3'.

[0067] Internal reference gene (GAPDH): Upstream: 5'-GGA GCG AGA TCC CTC CAA AAT-3', Downstream: 5'-GGCTGT TGT CAT ACT TCT CAT GG-3'.

[0068] The ability of the composition to activate opsins is represented by the upregulation of the opsin 4 (OPN4) and opsin 5 (OPN5) genes.

[0069] OPN4 promotion rate (%) = (OPN4 sample group - OPN4 blank control group) / OPN4 blank control group × 100%; OPN5 promotion rate (%) = (OPN5 sample group - OPN5 blank control group) / OPN5 blank control group × 100%.

[0070] Table 5 As shown in the table above, the composition provided in this application has a promotion rate of ≥40% for opsin 4 (OPN4) gene and ≥34% for opsin 5 (OPN5) gene. The composition provided in this application demonstrates strong opsin activation ability and can systematically address the overall decline in skin function caused by insufficient light signal input. This application provides a revitalizing and strengthening composition that can simulate beneficial light signals, activate and regulate the skin's opsin pathway, and specifically improve skin problems caused by prolonged exposure to indoor environments.

[0071] As can be seen from the comparison of Examples 1-8, ultrasonic extraction and heating reflux extraction can both be used to prepare mulberry root extract. However, the method for preparing mulberry root extract by ultra-high pressure and low temperature extraction provided in this application avoids thermal damage and fully preserves the active substances in mulberry root that have the ability to simulate light signals and activate the opsin 4 (OPN4) and opsin 5 (OPN5) pathways in skin cells, such as morin, morin bark extract and other flavonoid derivatives. The final extract obtained is significantly better than the product obtained by traditional methods in terms of light signal simulation efficiency and has a higher promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes.

[0072] As can be seen from the comparison of Examples 1 and 9-12, when the composition contains 1.2-1.8 parts of mulberry root extract, 0.5-1.5 parts of ceramide, 0.4-0.6 parts of polyglycerol-6 distearate, 0.4-0.6 parts of sorbitan olive oil ester, and 2.8-3.5 parts of hydrogenated lecithin, the promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes is higher, and the activation ability of opsins is stronger.

[0073] A comparison of Examples 1 and 13-15 shows that a specific ratio of ceramide NP and ceramide EOP can better repair the skin barrier, achieving immediate repair and long-lasting strengthening, and improving overall density. While mulberry root extract awakens the cell repair process, it provides the most suitable, biomimetic repair raw materials, resulting in a higher promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes. Examples 14-15 are outside the preferred range of ceramide NP and ceramide EOP (1-2):1, weakening the synergistic effect of the lipid barrier and slightly reducing the stability and density of the barrier structure, thus affecting the microenvironment for mulberry root extract to activate opsins, and therefore slightly impacting the opsin gene promotion rate.

[0074] As can be seen from the comparison between Example 1 and Comparative Example 1, when mulberry leaf extract was used instead of mulberry root extract in Comparative Example 1, although the main components in mulberry leaves (such as DNJ and rutin) have their biological activity, their effect on targeting and activating these types of opsin receptors is poor, so the promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes is not good.

[0075] As can be seen from the comparison of Example 1 and Comparative Examples 2-5, when other types of ceramides are selected or only one type of ceramide is selected, the anchoring of the barrier and the replenishment of moisture cannot be coordinated, and immediate repair and long-term toughness cannot be achieved. This affects the microenvironment for activating opsins by mulberry root extract, and also affects the input of active substances in mulberry root extract. The promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes is low, and the activation ability of opsins is poor.

[0076] As can be seen from the comparison of Example 1 and Comparative Examples 6-7, the presence of only polyglycerol-6 distearate or sorbitan oleate leads to a significant reduction in stability, transdermal permeability, activity protection, and mildness. The prepared nanoemulsion with a layered liquid crystal structure is unstable and cannot simultaneously possess structural stability and transdermal flexibility, affecting the input of active substances in mulberry root extract. The promotion rate of opsin 4 (OPN4) and opsin 5 (OPN5) genes is low, and the activation ability of opsins is poor.

[0077] Performance Test-2: Experiment on the promoting effect of the composition on the proliferation of skin keratinocytes.

[0078] The proliferative activity of keratinocytes (HaCaT) is a key indicator for assessing the skin's self-renewal and repair capabilities. More active cell proliferation indicates faster regenerative processes such as skin barrier reconstruction and wound healing. A higher cell proliferation rate after sample treatment indicates a more significant effect of the composition in promoting skin regeneration and enhancing the skin's self-repair capabilities.

[0079] Test substance preparation: Dilute the composition (Examples 1-15 and Comparative Examples 1-7) to 1.0% using DMEM low serum culture medium containing 1% fetal bovine serum as the final experimental concentration, and record it as the sample solution. Prepare and use immediately.

[0080] The cell line used in this invention is human immortalized keratinocyte HaCaT (Beina Biotechnology). The test conditions are: incubator temperature of 37±1℃, saturated humidity, and carbon dioxide of 5±1%.

[0081] Test method: Cell proliferation was detected using the CCK-8 assay, as detailed below: (1) Cell seeding: HaCaT cells in the logarithmic growth phase were digested and counted, and the cell density was adjusted to 5×10⁶ cells / year using DMEM high-glucose culture medium containing 10% fetal bovine serum. 4 Cells / mL. Seed the cell suspension at 100 μL per well in a 96-well cell culture plate, resulting in approximately 5000 cells per well. Pre-culture the plates in an incubator for 24 hours to allow complete cell adhesion.

[0082] (2) Cell treatment: Discard the original culture medium in each well. Add 100 μL of DMEM low serum culture medium containing 1% fetal bovine serum to the blank control group; add 100 μL of sample solution to each sample group. Set up 6 replicates for each group. Put the culture plate back into the incubator for further culture.

[0083] (3) CCK-8 assay: The assay was performed 24 hours after the drug treatment. During the assay, 10 μL of CCK-8 solution (Tongren Chemical, catalog number CK04) was added directly to each well, gently shaken to mix, and then placed back into the incubator for another 2 hours of incubation.

[0084] (4) Absorbance measurement and calculation: After incubation, the absorbance (OD value) of each well was measured at a wavelength of 450 nm using a microplate reader. The data was recorded.

[0085] The cell proliferation-promoting effect of the composition is expressed and compared by the cell proliferation rate, and the calculation formula is as follows: Cell proliferation rate (%) = (OD sample group – OD blank control group) / OD blank control group × 100%; The higher the cell proliferation rate, the stronger the effect of the composition in promoting cell proliferation.

[0086] The results are shown in Table 6.

[0087] Table 6 As shown in the table above, the composition provided in this application has a significant effect on the proliferation of keratinocytes, with a 24-hour cell proliferation rate ≥26.1%. Cell proliferation is active, and regenerative processes such as skin barrier reconstruction and wound healing are rapid, demonstrating a significant effect on the skin's self-repair ability. Among these, Figure 2 These are cell proliferation test images for Example 1 and Comparative Example 1, created by... Figure 2 As can be seen from the data, after 24 hours of culture, Example 1 significantly promoted the proliferation of keratinocytes.

[0088] As can be seen from the comparison of Examples 1-8, mulberry root extract can be prepared by ultrasonic extraction, heating and reflux extraction, etc. However, the preparation method of mulberry root extract by ultra-high pressure and low temperature extraction provided in this application avoids thermal damage and more effectively preserves the active substances in mulberry root that have the ability to simulate light signals and activate the opsin 4 (OPN4) and opsin 5 (OPN5) pathways in skin cells, such as morin, morin bark extract and other flavonoid derivatives. It also initiates intracellular calcium signaling and the MAPK pathway, upregulates cyclin expression, thereby accelerating keratinocyte proliferation and skin barrier reconstruction.

[0089] As can be seen from the comparison of Examples 1 and 9-12, when the composition contains 1.2-1.8 parts of mulberry root extract, 0.5-1.5 parts of ceramide, 0.4-0.6 parts of polyglycerol-6 distearate, 0.4-0.6 parts of sorbitan olive oil ester, and 2.8-3.5 parts of hydrogenated lecithin, the cell proliferation rate is higher, the cell proliferation is more active, the skin barrier reconstruction, wound healing and other regeneration processes are faster, and the skin's self-repair ability is more significant.

[0090] A comparison of Examples 1 and 13-15 shows that when ceramide NP and ceramide EOP are combined in a specific ratio, the regenerative processes such as skin barrier reconstruction and wound healing are accelerated, resulting in better repair of the skin barrier, achieving immediate repair and long-term strengthening, improving overall density, and increasing cell proliferation rate. Examples 14-15 are outside the preferred range of ceramide NP and ceramide EOP (1-2);1, leading to a weakened synergistic effect of the lipid barrier, a slight decrease in barrier structure stability and density, and thus affecting cell proliferation.

[0091] As can be seen from the comparison between Example 1 and Comparative Example 1, when mulberry leaf extract was replaced with mulberry root extract in Comparative Example 1, although the main components in mulberry leaves (such as DNJ and rutin) have their biological activity, their effect of targeting and activating opsin receptors is poor. They cannot initiate intracellular calcium signaling and the MAPK pathway, and cannot upregulate cyclin expression, which leads to accelerated keratinocyte proliferation and hinders skin barrier reconstruction, resulting in low cell proliferation rate.

[0092] As can be seen from the comparison of Example 1 and Comparative Examples 2-5, when other types of ceramides are selected or only one type of ceramide is selected, the components cannot form a synergy, the regeneration process such as skin barrier reconstruction and wound healing is hindered, and immediate repair and long-term toughness cannot be achieved, resulting in low cell proliferation rate.

[0093] As can be seen from the comparison of Example 1 and Comparative Examples 6-7, the presence of only polyglycerol-6 distearate or sorbitan oleate leads to a significant reduction in stability, transdermal permeability, activity protection, and mildness. The prepared nanoemulsion with a layered liquid crystal structure is unstable, unable to combine structural stability and transdermal flexibility, and cannot achieve both immediate repair and long-term toughness, resulting in low cell proliferation rate.

[0094] Performance testing - 3-spot patch test.

[0095] The efficacy examples of this invention verify the safety of its application on human skin. In accordance with the "Cosmetic Safety Technical Specifications" (2015) for human skin patch testing, 33 volunteers aged 18-60 years were recruited. The test environment was a temperature of (21±1)℃ and a humidity of (50±10)%.

[0096] Volunteers washed their forearms with clean water. After 5-10 minutes, once the moisture had evaporated, the testers applied 0.020-0.025 mL of each sample to each chamber of a 10-well spot tester. The inner sides of both forearms of the volunteers were selected, avoiding the skin around the joints of the hands. A spot tester was applied to each arm and marked accordingly. The spots were left on for 24 hours. After removing the spot testers and wiping away any remaining sample, skin reactions were observed at 0.5h, 24h, and 48h. The results were recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Specifications" (2015). As shown in Table 7, skin reactions were divided into four rating levels; the higher the score, the more severe the skin irritation.

[0097] Table 7 The test results are as follows: 0 cases of withdrawal and 0 cases of adhesive tape allergy were observed in the technical solution provided by this invention. No adverse reactions occurred in the application case, control application case, and blank application case.

[0098] Performance testing - 4 Human efficacy experiments.

[0099] We recruited 72 volunteers who worked indoors for about 8 hours a day and felt they had sensitive skin (lactate sting test score ≥3). There were 36 male and 36 female volunteers, aged between 25 and 40, and they were randomly divided into 24 groups of 3 people each. The recruited Asian adult volunteers, under normal circumstances, continuously used the product on their entire face for 14 days. Every morning and evening after cleansing and moisturizing, the volunteers took about 1g of face cream (application examples 1-16, control examples 1-7 and blank examples) and applied it to their face, massaging it in until absorbed. The product was used on the entire face once in the morning and once in the evening, and a follow-up visit was conducted after 14 days.

[0100] On the day of their visit, volunteers washed their faces with water in the test area without applying any products and sat quietly for 20 minutes in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10%.

[0101] The improvement of facial skin is assessed using instruments, including the Tewameter. TM Hex assay for transdermal water loss (TEWL), Colorimeter CL 400 measurement of forehead color (ITA° value) and cheekbone brightness (L* value), and changes in lactic acid sting test scores. VISIA-CR was used to photograph and analyze the volunteers' skin texture.

[0102] The skin tolerance test, also known as the lactic acid stinging test, was conducted as follows: 1g of the test group sample was applied to one side of the volunteer's face and allowed to be absorbed. A 10% lactic acid solution was applied to the nasolabial folds of each subject's face (using a 0.8cm diameter double-layered filter paper) for 5 minutes. After 5 minutes, the solution was removed to assess the sensitivity of the face to lactic acid stimulation. A 4-point questionnaire was used to assess the sensitivity score. The questionnaire asked: How do you perceive the stinging sensation on your face at this moment? 0 - No sensation, 1 - Slight stinging, 2 - Significant stinging, 3 - Severe stinging. The lactic acid stinging score was calculated based on the scores of each group. TEWL and skin texture score improvement rate = (mean before use - mean after use) / mean before use × 100%; ITA and brightness L* improvement rate = (mean after use - mean before use) / mean before use × 100%; Lactic acid stinging score = average score of each group / number of people in each group, specifically, average score of 3 subjects / 3; The results are shown in Table 8. The improvement rate presented in Table 8 is the improvement rate at the follow-up visit on day 14.

[0103] Table 8 As shown in the table above, the composition provided in this application, when prepared into an application-specific face cream, further enhances skin tolerance, repairs the skin barrier, improves skin tone, and enhances self-repair ability. Volunteers using the face cream provided in this application showed a TEWL improvement rate ≥21.8%, a skin color improvement rate ≥15.4%, a skin tone brightness improvement rate ≥12.1%, a skin texture improvement rate ≥13.2%, and a skin lactic acid stinging score ≤1.3. Among these, Figure 3 To illustrate the human body function diagram in Example 1, from... Figure 3The results show that after 14 days of use, the volunteers' skin became whiter and their skin texture became finer.

[0104] As can be seen from the comparison of application examples 1-8, ultrasonic extraction and heating reflux extraction can both be used to prepare mulberry root extract. However, the method for preparing mulberry root extract by ultra-high pressure low temperature extraction provided in this application avoids thermal damage and fully preserves the active substances in mulberry root that have the ability to simulate light signals and activate the opsin 4 (OPN4) and opsin 5 (OPN5) pathways in skin cells. The resulting extract has better effects in improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability.

[0105] As can be seen from the comparison of Application Examples 1 and 9-12, when the composition contains 1.2-1.8 parts of mulberry root extract, 0.5-1.5 parts of ceramide, 0.4-0.6 parts of polyglycerol-6 distearate, 0.4-0.6 parts of sorbitan olive oil ester, and 2.8-3.5 parts of hydrogenated lecithin, the effects of improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability are better.

[0106] As can be seen from the comparison of application examples 1 and 13-15, the combination of ceramide NP and ceramide EOP in a specific ratio can better repair the skin barrier, achieve immediate repair and long-term toughness, improve overall density, thereby improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability.

[0107] As can be seen from the comparison between Application Example 1 and Comparative Application Example 1, when mulberry leaf extract was replaced with mulberry root extract in Comparative Example 1, the improvement rates of various aspects decreased significantly, while the skin lactic acid stinging score increased. This indicates that the unique active ingredients in mulberry root are the key material basis for activating the skin's self-repair pathway and achieving barrier repair and skin tone improvement.

[0108] As can be seen from the comparison of Application Example 1 and Comparative Application Examples 2-5, when other types of ceramides are selected or only one type of ceramide is selected, the components cannot form a synergy, and cannot achieve immediate repair and long-term toughness. It also affects the input of active substances in mulberry root extract, and the effects of improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability are extremely poor.

[0109] As can be seen from the comparison of Application Example 1 and Comparative Application Examples 6-7, when only polyglycerol-6 distearate or sorbitan olive oil ester is contained, the prepared nanoemulsion cannot simultaneously possess structural stability and transdermal flexibility, which affects the input of active substances in mulberry root extract and results in extremely poor effects in improving skin tolerance, repairing the skin barrier, improving skin tone, and enhancing self-repair ability.

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. A revitalizing and strengthening composition, characterized in that, It includes the following components in parts by weight: 1-2 parts of mulberry root extract, 0.1-2 parts of ceramide, 0.3-0.8 parts of polyglycerol-6 distearate, 0.3-0.8 parts of sorbitan olive oil ester, and 2.5-4 parts of hydrogenated lecithin; The ceramide is a mixture of ceramide EOP and ceramide NP; The revitalizing and strengthening composition is a nanoemulsion with a layered liquid crystal structure. The nanoemulsion includes an oil phase and an aqueous phase. The oil phase includes ceramide, polyglycerol-6 distearate, sorbitan olive oil ester, and hydrogenated lecithin. The aqueous phase includes mulberry root extract.

2. The revitalizing and strengthening composition according to claim 1, characterized in that, It includes the following components in parts by weight: 1.2-1.8 parts of mulberry root extract, 0.5-1.5 parts of ceramide, 0.4-0.6 parts of polyglycerol-6 distearate, 0.4-0.6 parts of sorbitan olive oil ester, and 2.8-3.5 parts of hydrogenated lecithin.

3. The revitalizing and strengthening composition according to claim 1, characterized in that, The weight ratio of ceramide NP to ceramide EOP is (0.5-5):

1.

4. The revitalizing and strengthening composition according to claim 1, characterized in that, The preparation method of the mulberry root extract includes the following steps: The mulberry root is crushed and sieved to obtain mulberry root powder; The mulberry root powder is mixed with an organic solvent and subjected to ultra-high pressure and low temperature extraction. After extraction, the filtrate is collected. The pressure of the ultra-high pressure and low temperature extraction is 300-500 MPa, and the temperature of the ultra-high pressure and low temperature extraction is 25-35℃. The filtrate was concentrated to a paste and freeze-dried to constant weight to obtain the mulberry root extract.

5. The revitalizing and strengthening composition according to claim 1, characterized in that, The sieve mesh size is 20-50 mesh; And / or, the extraction time is 5-15 min; And / or, the organic solvent is an ethanol solution with a volume fraction of 40-60%; And / or, the mass-to-volume ratio of the mulberry root powder to the organic solvent is 1 g: (10-15) mL; And / or, the ultra-high pressure low temperature extraction is performed 1-3 times.

6. A method for preparing the revitalizing and strengthening composition according to any one of claims 1-5, characterized in that, Includes the following steps: S1. The ceramide, polyglycerol-6 distearate, sorbitan olive oil ester and hydrogenated lecithin are heated and mixed evenly to obtain the oil phase; S2. Mix the mulberry root extract with heated water until homogeneous to obtain an aqueous phase; S3. The oil phase is added dropwise to the aqueous phase, and shearing is performed to form a nanoemulsion; S4. After homogenizing the nanoemulsion, cool it, adjust the pH value, and filter to obtain the revitalizing and toughening composition.

7. The method for preparing the revitalizing and strengthening composition according to claim 6, characterized in that, In step S1, the temperature is heated to 60-80°C, the mixing speed is 300-1000 rpm, and the mixing time is 10-30 min. And / or, in S2, the water is heated to 60-80°C, the mixing speed is 300-1000 rpm, and the mixing time is 2-5 min; And / or, in S3, the shearing rotation speed is 8000-20000 rpm, the shearing time is 5-15 min, and the shearing temperature is 60-80℃; And / or, in S4, the homogenization pressure is 1000-1500 bar, the homogenization is performed 2-4 times, the mixture is cooled to room temperature, and the pH value is adjusted to 5.0-7.

0.

8. The use of the revitalizing and strengthening composition according to any one of claims 1-5 in the preparation of cosmetics.

9. A cosmetic product, characterized in that, The cosmetic product includes the revitalizing and strengthening composition as described in any one of claims 1-5.

10. The cosmetic product as described in claim 9, characterized in that, The cosmetics include one of the following: toner, lotion, cream, mask, and serum.

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