CHI3L1 protein detection method based on micro-flow optical fiber sensing
By immobilizing CHI3L1 antibodies on the inner wall of a microfluidic channel using microfluidic fiber optic sensing technology, and combining this with a broadband light source and single-mode optical fiber, the problems of insufficient sensitivity and operational complexity of existing CHI3L1 detection technologies have been solved. This enables highly sensitive and rapid detection of CHI3L1 protein, supporting the early diagnosis and dynamic monitoring of liver fibrosis.
CN122084550APending Publication Date: 2026-05-26南昌大学第一附属医院
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Patent Information
- Application Number
- CN202610322287.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-17
- Publication Date
- 2026-05-26
AI Technical Summary
Technical Problem
Existing CHI3L1 detection technologies suffer from insufficient sensitivity, cumbersome and time-consuming detection processes, and low system integration, making it difficult to achieve early diagnosis and dynamic monitoring of liver fibrosis.
Method used
A microfluidic fiber optic sensing method was adopted to achieve high-sensitivity and rapid detection of CHI3L1 protein by immobilizing a CHI3L1 protein-specific antibody on the inner wall of a microfluidic channel, combined with a broadband light source and a single-mode optical fiber.
Benefits of technology
It achieves highly sensitive detection of CHI3L1 protein, simplifies the operation process, is suitable for portable devices, and supports early diagnosis and dynamic monitoring of liver fibrosis.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a CHI3L1 protein detection method based on micro-flow optical fiber sensing, and belongs to the technical field of biomedical detection. The method comprises the following steps: providing a microstructure optical fiber sensing unit with a through microfluidic channel; fixing a CHI3L1 protein specific antibody on the inner surface of the microfluidic channel; a sample to be detected is injected into the channel, so that CHI3L1 protein is specifically captured; quantitative detection of CHI3L1 protein is realized by detecting the characteristic parameter drift amount of an optical fiber transmission spectrum caused by antigen-antibody combination. According to the invention, the microfluidic technology is combined with optical fiber sensing, the interaction efficiency of a light field and biomolecules is increased by fixing the antibody on the inner wall of the microfluidic channel, the detection sensitivity is obviously improved, and the optical fiber sensor has the advantages of rapid detection, less sample consumption and no label, and is suitable for early non-invasive screening and dynamic monitoring of clinical hepatic fibrosis.
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