Titration analysis methods, systems, equipment, and media based on continuous flow and spectral detection

Abstract

This invention provides a titration analysis method, system, device, and medium based on continuous flow and spectral detection. The method includes: controlling a first precision pump to transport the sample to be tested through a first injection line and a second precision pump to transport the titrant through a second injection line into a mixing device to obtain a mixing result; controlling the mixing result to pass through a microflow cell and obtaining a spectral signal using a light source and a spectrometer; monitoring abrupt changes in the mixing result based on the spectral signal to obtain the mixing state; when the mixing state does not meet preset conditions, the mixing result in the microflow cell is refluxed back into the mixing device through a reflux device, and the second precision pump is controlled to continue transporting the titrant into the mixing device until the mixing state of the remixed result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint corresponding to the sample under the titrant is determined based on the spectral signal; and the target concentration corresponding to the sample is calculated based on the titration endpoint.

Description

Technical Field

[0001] This invention relates to the field of chemical analysis technology, and in particular to a titration analysis method, system, device and medium based on continuous flow and spectral detection. Background Technology

[0002] Chemical titration is a classic quantitative analysis method. Traditional manual titration is cumbersome, inefficient, and prone to human error. Even with the introduction of spectroscopic detection to improve endpoint accuracy, placing it in a batch reaction vessel still fails to break through the existing paradigm. Existing technologies generally suffer from low throughput, high reagent consumption, difficulty in online integration, and poor mixing reproducibility. These factors collectively limit its application in modern industrial analysis and trace detection. Therefore, there is an urgent need for a titration analysis method based on continuous flow and spectroscopic detection to solve the problems of slow analysis speed, high reagent consumption, difficulty in automated online monitoring, and inconsistent mixing efficiency in existing technologies. Summary of the Invention

[0003] The main objective of this invention is to provide a titration analysis method, system, device, and medium based on continuous flow and spectral detection, aiming to solve problems such as slow analysis speed, high reagent consumption, difficulty in automated online monitoring, and inconsistent mixing efficiency in the prior art.

[0004] In a first aspect, embodiments of the present invention provide a titration analysis method based on continuous flow and spectral detection, applied to a titration analysis system based on continuous flow and spectral detection. The system includes at least a first precision pump and a second precision pump. The first precision pump is used to deliver the sample to be tested, and the second precision pump is used to deliver the titrant. A mixing device is provided, with the first precision pump connected to the mixing device via a first injection line, and the second precision pump connected to the mixing device via a second injection line. A light source is provided, and a microflow cell, a spectrometer, and a reflux device are provided downstream of the mixing device. The method includes: The first precision pump and the second precision pump are controlled to respectively transport the sample to be tested and the titrant into the mixing device to obtain the mixing result; The mixing result is controlled to pass through the microflow cell and a spectral signal is obtained using the light source and the spectrometer; Based on the spectral signal, abrupt changes in the mixing result in the mixing device are monitored to obtain the mixing state corresponding to the mixing result; When the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue to deliver the titrant into the mixing device until the mixing state of the re-mixed mixing result meets the preset conditions. When the mixing state meets the preset conditions, the titration endpoint of the sample to be tested under the titrant is determined according to the spectral signal. Calculate the target concentration of the sample to be tested based on the titration endpoint.

[0005] Secondly, embodiments of the present invention provide a titration analysis system based on continuous flow and spectral detection. The system includes at least a first precision pump and a second precision pump. The first precision pump is used to deliver the sample to be tested, and the second precision pump is used to deliver the titrant. A mixing device is provided, with the first precision pump connected to the mixing device via a first injection line, and the second precision pump connected to the mixing device via a second injection line. A light source is provided, and a microflow cell, a spectrometer, and a reflux device are located downstream of the mixing device. The system also includes a controller, which executes: The first and second precision pumps are controlled to transport the sample to be tested and the titrant into the mixing device to obtain a mixing result; the mixing result is controlled to pass through the microflow cell and a spectral signal is obtained using the light source and the spectrometer; the mixing result in the mixing device is monitored for abrupt changes based on the spectral signal to obtain the mixing state corresponding to the mixing result; when the mixing state does not meet the preset conditions, the mixing result in the microflow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue transporting the titrant into the mixing device until the mixing state of the re-mixed result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint corresponding to the sample to be tested under the titrant is determined based on the spectral signal; the target concentration corresponding to the sample to be tested is calculated based on the titration endpoint.

[0006] Thirdly, embodiments of the present invention also provide a terminal device, the terminal device including a processor, a memory, a computer program stored in the memory and executable by the processor, and a data bus for realizing communication between the processor and the memory, wherein when the computer program is executed by the processor, it implements the steps of any of the titration analysis methods based on continuous flow and spectral detection provided in this specification.

[0007] Fourthly, embodiments of the present invention also provide a storage medium for computer-readable storage, characterized in that the storage medium stores one or more programs, which can be executed by one or more processors to implement the steps of any of the titration analysis methods based on continuous flow and spectral detection provided in this specification.

[0008] This invention provides a titration analysis method, system, device, and medium based on continuous flow and spectral detection. The system is applied to a titration analysis system based on continuous flow and spectral detection. The system includes at least a first precision pump and a second precision pump. The first precision pump delivers the sample to be tested, and the second precision pump delivers the titrant. A mixing device is included. The first precision pump is connected to the mixing device via a first injection line, and the second precision pump is connected to the mixing device via a second injection line. A light source is provided. Downstream of the mixing device, a microflow cell, a spectrometer, and a reflux device are provided. The method includes controlling the first and second precision pumps to respectively deliver the sample to be tested and the titrant into the mixing device. The method involves obtaining a mixing result; controlling the mixing result through a micro-flow cell and obtaining spectral signals using a light source and spectrometer; monitoring abrupt changes in the mixing result based on the spectral signals to obtain the corresponding mixing state; when the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-entered into the mixing device through a reflux device, and the second precision pump continues to deliver titrant into the mixing device until the mixing state of the re-mixed result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint of the test sample under the titrant is determined based on the spectral signal; and the target concentration of the test sample is calculated based on the titration endpoint. This method accurately delivers the test sample and titrant to the test sample using a first and second precision pump, respectively. Combined with a mixing device, micro-flow cell, spectrometer, and reflux device, it can monitor the mixing state in real time and automatically reflux and re-titrate mixtures that do not meet the conditions until the preset conditions are met, thus significantly improving the automation, reaction sufficiency, and result repeatability of the titration process. Simultaneously, it accurately identifies the titration endpoint and calculates the target concentration based on the spectral signal, avoiding subjective errors from manual endpoint judgment and improving detection sensitivity, data reliability, and analytical efficiency. Furthermore, by replacing traditional intermittent operation with a titration analysis system based on continuous flow and spectral detection, the bottlenecks in analysis speed and throughput are fundamentally solved; precise micro-flow control significantly reduces reagent and sample consumption. It also addresses problems in existing technologies such as slow analysis speed, high reagent consumption, difficulty in automated online monitoring, and inconsistent mixing efficiency. Attached Figure Description

[0009] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0010] Figure 1 A schematic diagram of the module structure of a titration analysis system based on continuous flow and spectral detection provided in an embodiment of the present invention; Figure 2A schematic flowchart of a titration analysis method based on continuous flow and spectral detection provided in an embodiment of the present invention; Figure 3 A schematic diagram of the module structure of another titration analysis system based on continuous flow and spectral detection provided in an embodiment of the present invention; Figure 4 This is a schematic block diagram of a terminal device provided in an embodiment of the present invention. Detailed Implementation

[0011] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0012] The flowchart shown in the attached diagram is for illustrative purposes only and does not necessarily include all content and operations / steps, nor does it necessarily have to be performed in the order described. For example, some operations / steps can be broken down, combined, or partially merged, so the actual execution order may change depending on the actual situation.

[0013] It should be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0014] This invention provides a titration analysis method, system, device, and medium based on continuous flow and spectral detection. The titration analysis method based on continuous flow and spectral detection can be applied to terminal devices, such as tablet computers, laptops, desktop computers, personal digital assistants, and wearable devices. The terminal device can be a server or a server cluster.

[0015] The following detailed description of some embodiments of the present invention is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0016] Please refer to Figure 1From the perspective of different structural classifications, the titration analysis system 100 based on continuous flow and spectral detection includes: at least a first precision pump 101 and a second precision pump 102, wherein the first precision pump 101 is used to deliver the sample to be tested, and the second precision pump 102 is used to deliver the titrant; a mixing device 103 is provided, wherein the first precision pump 101 is connected to the mixing device 103 through a first injection line 1011, and the second precision pump 102 is connected to the mixing device 103 through a second injection line 1021; a light source 104 is provided; a micro-flow cell 105, a spectrometer 106, and a reflux device 108 are provided downstream of the mixing device 103; the system 100 also includes a controller 107, which controls the first precision pump 101 and the second precision pump 102 to respectively deliver the sample to be tested and the titrant into the mixing device 101. 3. Obtain the mixing result; control the mixing result to pass through the micro-flow cell 105 and obtain the spectral signal using the light source 104 and the spectrometer 106; monitor the abrupt change of the mixing result in the mixing device 103 according to the spectral signal to obtain the mixing state corresponding to the mixing result; when the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell 105 is re-flowed into the mixing device 103 through the reflux device 108, and the second precision pump 102 is controlled to continue to deliver the titrant into the mixing device 103 until the mixing state of the re-mixed mixing result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint corresponding to the test sample under the titrant is determined according to the spectral signal; the target concentration corresponding to the test sample is calculated according to the titration endpoint.

[0017] For example, the system 100 is provided with at least two precision pumps, a first precision pump 101 and a second precision pump 102. The first precision pump 101 is used to deliver the sample to be tested at a constant flow rate, and the second precision pump is used to titrate the reagent at a constant flow rate.

[0018] For example, the system 100 includes an injection line connected to the first precision pump 101 and the second precision pump 102, and a mixing device 103. The mixing device 103, such as a coil mixer or a static mixer, is used to continuously mix the sample to be tested and the titrant to induce a chemical reaction. The first precision pump 101 is connected to the mixing device 103 via a first injection line 1011, and the second precision pump 102 is connected to the mixing device 103 via a second injection line 1021.

[0019] For example, the system 100 also includes a microflow cell 105, a spectrometer 106, a light source 103, and a reflux device 108. The microflow cell 105 is located downstream of the mixing device 103, and its optical window is located in the optical path of the spectrometer 106 for real-time detection of the spectral signal of the mixture flowing through it. The optical window ensures that the detection light from the light source can effectively pass through the mixed sample in the mixing device 103 and be received by the spectrometer 106, thereby providing good support for the subsequent controller 107 to realize real-time, online spectral detection of the fluid mixture.

[0020] Please refer to Figure 2 , Figure 2 This is a schematic flowchart of a titration analysis method based on continuous flow and spectral detection provided in an embodiment of the present invention.

[0021] like Figure 2 As shown, the titration analysis method based on continuous flow and spectral detection includes steps S201 to S206.

[0022] Step S201: Control the first precision pump and the second precision pump to transport the sample to be tested and the titrant into the mixing device to obtain the mixing result.

[0023] For example, the controller 107 controls the first precision pump 101 to transport the sample to be tested through the first injection line 1011 into the mixing device 103 according to the program design, and at the same time controls the second precision pump 102 to transport the titrant through the second injection line 1021 into the mixing device 103, so that the sample to be tested and the titrant are mixed in the mixing device 103 and a chemical reaction occurs to obtain a mixing result.

[0024] In some embodiments, controlling the first precision pump and the second precision pump to respectively transport the test sample and the titrant into the mixing device to obtain a mixing result includes: controlling a first flow rate of the test sample into the mixing device through the first precision pump and the first injection line; controlling a second flow rate of the titrant into the mixing device through the second precision pump and the second injection line; wherein the first flow rate and the second flow rate are both constant; when the test sample reaches a first flow rate at the first flow rate, sampling of the test sample is stopped, and pure water is continued to be injected into the mixing device at the first flow rate through the first precision pump and the first injection line; when the test sample reaches the first flow rate at the first flow rate and the titrant reaches the second flow rate at the second flow rate, air bubbles are added using the first precision pump or the second precision pump for isolation, and the mixing result of the test sample and the titrant in the mixing device is determined.

[0025] For example, a dual-channel peristaltic pump is used with a first precision pump and a second precision pump, and an ultraviolet-visible spectrometer is used to detect the wavelength at 550 nm.

[0026] For example, the sample to be tested is a diluted fruit juice sample, and the titrant is a NaOH standard solution containing phenolphthalein indicator. The first flow rate of the diluted fruit juice sample into the mixing device 103 is controlled by the first precision pump 101 and the first injection line 1011, and the second flow rate of the NaOH standard solution containing phenolphthalein indicator into the mixing device 103 is controlled by the second precision pump 102 and the second injection line 1021. The first flow rate and the second flow rate are both constant, that is, the first flow rate and the second flow rate are both unchanged.

[0027] For example, the test sample and titrant are transported at a first flow rate and a second flow rate, thereby mixing the test sample and titrant in a mixing device to obtain the corresponding mixing result. The mixing result is the result of a chemical reaction between the test sample and the titrant.

[0028] For example, after the diluted fruit juice sample and the NaOH standard solution containing phenolphthalein indicator are mixed through the T-joint, they flow into a 2-meter-long coil (0.5 mm inner diameter), i.e., the mixing device 103, for reaction.

[0029] It should be noted that the length of the coil can be appropriately shortened or extended according to the requirements of the chemical reaction. This application does not impose specific limitations on the length of the coil; it can be set according to actual needs.

[0030] For example, a first precision pump and a second precision pump are set to operate in a constant working mode on the first and second injection lines, respectively. The first flow rate of the sample and the second flow rate of the titrant are precisely controlled by adjusting the pump speed, ensuring that these two flow rates remain stable throughout the injection process. When the sample is continuously delivered at the first flow rate to reach the preset first flow rate, pure water is injected at the same speed after sampling. When the titrant is continuously delivered at the second flow rate to reach the preset second flow rate, a certain volume of mixture will be formed in the mixing device. To clearly distinguish this mixture at a specific mixing ratio from subsequent mixtures, the first or second precision pump needs to be briefly started to draw in a section of air bubbles, such as air. The air bubbles act as a separating medium to physically separate the current mixture into an independent reaction section in the pipeline. The liquids before and after the air bubbles will not mix, thus the mixture separated by the air bubbles can be accurately taken as the mixture of the sample and the titrant at the currently set flow rates and volumes.

[0031] Furthermore, the bubbles can be injected immediately after the sample and titrant are drawn in by the first and second precision pumps, allowing each segment of the liquid flow to mix and react independently. The bubbles are also injected at a constant rate, with intervals of several seconds, typically 2 seconds (this can be controlled by an air valve; longer intervals may result in less uniform mixing). A spectrometer monitors the spectral signal of each liquid flow segment, thus obtaining a good signal over a certain period of time.

[0032] Specifically, by precisely controlling the injection volume with a constant flow rate and using bubbles to physically isolate the mixing results, cross-contamination can be avoided, ensuring that each reaction stage is independent and identifiable. At the same time, constant flow and fixed flow rate ensure high repeatability of the mixing ratio, providing a stable environment for spectral monitoring, thereby improving titration accuracy and reliability.

[0033] In some embodiments, controlling the first precision pump and the second precision pump to respectively transport the test sample and the titrant into the mixing device to obtain a mixing result includes: controlling a first flow rate of the test sample into the mixing device through the first precision pump and the first injection line, and maintaining the first flow rate constant; controlling a second flow rate of the titrant into the mixing device through the second precision pump and the second injection line, and controlling the concentration change of the titrant according to the second flow rate; when the test sample reaches a first flow rate at the first flow rate, stopping the sampling of the test sample, and continuing to inject pure water into the mixing device at the first flow rate through the first precision pump and the first injection line; when the test sample reaches the first flow rate at the first flow rate and the titrant reaches a second flow rate at the second flow rate, adding air bubbles using the first precision pump or the second precision pump for isolation, and determining the mixing result of the test sample and the titrant in the mixing device under the first flow rate, the second flow rate, and the concentration change of the titrant.

[0034] For example, before the experiment begins, the first and second precision pumps need to be calibrated. By measuring the mass or volume of pure water delivered per unit time at a set speed, a precise correspondence between pump speed / frequency and actual flow rate is established, ensuring the accuracy of flow rate control.

[0035] For example, baseline correction and wavelength accuracy verification were performed on the ultraviolet-visible spectrometer. After selecting 550 nm as the detection wavelength, the instrument's response stability could be confirmed using standard filters or standard solutions.

[0036] For example, based on the estimated concentration range of the sample to be tested, the required detection sensitivity, and the system pressure, a constant flow rate, i.e., a first flow rate, is determined and set for the first precision pump. The first flow rate remains constant during a single titration.

[0037] For example, the initial flow rate of the titrant is determined. To achieve concentration changes, the flow rate of the second precision pump is not constant, but rather a core variable that changes according to a preset program; that is, the second flow rate is the rate of change.

[0038] For example, a pattern of titrant concentration variation is determined. In a continuous flow system, the effective concentration variation of the titrant is achieved by changing its flow rate ratio relative to the sample. This is done by controlling the flow rate of a second precision pump, starting from zero or a low value, and increasing linearly or stepwise over time. In this way, the instantaneous proportion of the titrant in the mixture stream continuously increases over time.

[0039] For example, a first precision pump and a second precision pump are started simultaneously to deliver fluid at a set first flow rate and an initial second flow rate, respectively. The rotational speed of the second precision pump is then adjusted automatically and in real time according to a preset program, thereby precisely controlling the second flow rate to increase linearly according to a predetermined pattern. The first flow rate remains constant throughout.

[0040] For example, the sample to be tested and the titrant are initially combined through a T-joint, and then enter a long coil mixer, i.e., a mixing device. Inside the mixing device, the laminar diffusion of the fluid and the secondary flow caused by the curvature of the coil achieve full and uniform mixing and reaction, forming a stable "mixing result" plug flow.

[0041] For example, a first precision pump and a second precision pump are configured to operate in the aforementioned working mode on the first and second injection lines, respectively. The first flow rate of the sample and the second flow rate of the titrant are precisely controlled by adjusting the pump speed, ensuring that the first flow rate remains stable throughout the injection process and that the second flow rate changes according to a preset program. Thus, when the sample is continuously delivered at the first flow rate to reach the preset first flow rate, after sampling, the first precision pump and the first injection line inject pure water at the same speed, while the titrant is continuously delivered at the second flow rate to reach the preset second flow rate. At this point, a certain volume of mixture will be formed in the mixing device. To clearly distinguish this mixture at a specific mixing ratio from subsequent mixtures, the first or second precision pump needs to be briefly activated to draw in a section of air bubbles, such as air. These air bubbles act as a separating medium, physically dividing the current mixture into an independent reaction section within the pipeline. The liquids before and after this air bubble will not mix, thus accurately representing the mixture separated by the air bubble as the mixture of the sample and titrant at the currently set flow rate and flow rate.

[0042] Furthermore, the bubbles can be injected immediately after the sample and titrant are drawn in by the first and second precision pumps, allowing each segment of the liquid flow to mix and react independently. The bubbles are also injected at a constant rate, with intervals of several seconds, typically 2 seconds (this can be controlled by an air valve; longer intervals may result in less uniform mixing). A spectrometer monitors the spectral signal of each liquid flow segment, thus obtaining a good signal over a certain period of time.

[0043] Specifically, by combining a constant sample flow rate with a programmed change in the titrant flow rate, a linear or stepwise concentration gradient can be automatically and in real time generated in a continuous flow system, significantly improving the efficiency and resolution of titration analysis. Furthermore, the introduction of air bubbles as a physical barrier precisely separates the reaction section at a specific mixing ratio from the subsequent system, avoiding diffusion and mixing interference, and ensuring that each mixing result can independently and accurately represent the sample-titer interaction at the corresponding moment.

[0044] Step S202: Control the mixing result to pass through the micro-flow cell and obtain a spectral signal using the light source and the spectrometer.

[0045] For example, the mixing result is controlled to flow into a micro-flow cell, and then the corresponding spectral signal is obtained by using a spectrometer to collect the corresponding signal after the micro-flow cell is irradiated by a light source.

[0046] In some embodiments, obtaining a spectral signal using the light source and the spectrometer includes: controlling the light source so that the light emitted by the light source passes through the optical window corresponding to the micro-flow cell along a designed optical path, and receiving the corresponding spectral signal using the spectrometer.

[0047] For example, the mixing result is controlled to flow into a micro-flow cell, so that the light emitted by the light source passes through the optical window corresponding to the micro-flow cell along the designed optical path and is emitted out. Then, the spectrometer collects the emitted light and receives the corresponding spectral signal.

[0048] Step S203: Based on the spectral signal, perform abrupt change monitoring on the mixing result in the mixing device to obtain the mixing state corresponding to the mixing result.

[0049] For example, the spectrometer receives the corresponding spectral signal, and then converts the spectral signal representing the mixing uniformity output by the spectrometer into an analog voltage signal and sends it to a voltage comparator for comparison with a preset reference voltage representing a threshold of fully mixed state; if the comparator outputs a low level (not fully mixed), the mixing state is determined to be not fully mixed; otherwise, it is fully mixed, wherein the mixing state is either fully mixed or not fully mixed.

[0050] Step S204: When the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue to deliver the titrant into the mixing device until the mixing state of the re-mixed mixing result meets the preset conditions.

[0051] For example, if the preset condition is that the mixing state is fully mixed, then when the mixing state is not fully mixed, it means that the mixing state does not meet the preset condition, and the liquid in the micro-flow cell is returned to the mixing device via the reflux device.

[0052] For example, the absorbance or spectral characteristics of the effluent are continuously monitored using a UV-Vis spectrometer at a microflow cell. A pre-set threshold is used, such as an absorbance fluctuation of less than 0.5% to indicate "sufficient mixing." When the spectrometer reading shows large fluctuations or unstable signals, indicating "insufficient mixing," the controller switches the multi-port valve at the outlet of the microflow cell, preventing the liquid from flowing to waste liquid. Instead, the liquid is redirected to the mixing device, where a reflux device powers the return of the insufficiently mixed liquid back into the mixing device.

[0053] Furthermore, when the mixture is returned to the mixing apparatus, the separating media that physically divide the current mixture into multiple independent reaction sections are retained. That is, the separating media, such as air bubbles, remain and are not disrupted when titrant is added to the mixture.

[0054] For example, during the process of pumping the mixture, which includes air bubbles, back into the mixing device, it is necessary to ensure that the air bubbles are not broken. The second precision pump is controlled to continue delivering titrant to the mixing device according to a preset program. After the titrant-added mixture (still segmented by air bubbles) flows out of the mixing device, it re-enters the microflow cell. At this point, the absorbance or spectrum is repeatedly acquired using the same light source and spectrometer. If the preset conditions are still not met, the reflux device is restarted, and the above steps (continue adding titrant → reflux mixing → re-detection) are repeated until the preset conditions are met.

[0055] Furthermore, if the reaction endpoint is not reached after the first round of titrant mixing, the solution is returned to the first precision pump. At this point, no pure water is injected, and the next round of titrant mixing begins until the reaction is complete. The first precision pump has a switching injection selection function.

[0056] Step S205: When the mixing state meets the preset conditions, the titration endpoint of the sample to be tested under the titrant is determined according to the spectral signal.

[0057] For example, when the mixing state is fully mixed, the maximum value of the derivative is found in the first derivative curve based on the abruptly rising curve in the spectral signal; for the abruptly falling titration curve, the minimum value of the derivative is found, and the extreme point is the titration endpoint.

[0058] For example, three regions are identified on the spectral signal, including the pre-jump plateau region (front linear region), the jump region (rapid change region), and the post-jump plateau region (back linear region). Then, linear fitting is performed on the front linear region and the back linear region respectively to solve for the intersection of the two fitted lines. The intersection of the two fitted lines is then determined as the titration endpoint.

[0059] For example, taking a fruit juice sample as an example, the absorbance (550 nm) at different time points and the corresponding cumulative volume ratio of NaOH are recorded to plot the titration curve between absorbance and time. Phenolphthalein is colorless under acidic conditions. As NaOH is added, the pH increases, and phenolphthalein turns pink, and the absorbance increases. Near the equivalence point, the absorbance rises rapidly, forming an S-shaped curve. The first derivative can be calculated to find the maximum value point, or the second derivative can be calculated to find the zero-crossing point, which is the titration endpoint.

[0060] Step S206: Calculate the target concentration of the sample to be tested based on the titration endpoint.

[0061] For example, if the titration endpoint represents the sampling time, then the cumulative flow rate and sample flow rate are calculated based on the titration endpoint to determine the target concentration of the sample to be tested. If the titration endpoint represents the data concentration, then the concentration information corresponding to the inflection point is the stoichiometric concentration, and the target concentration corresponding to the target data contained in the sample to be tested is calculated accordingly.

[0062] In some embodiments, calculating the target concentration of the test sample based on the titration endpoint includes: when the titration endpoint represents time information, determining the sample flow rate and the cumulative flow rate of the titrant based on the first velocity corresponding to the test sample and the second velocity corresponding to the titrant, combined with the titration endpoint; calculating the target concentration of the test sample based on the sample flow rate and the cumulative flow rate; when the titration endpoint is used to represent the stoichiometric concentration of the titrant, determining the target concentration of the test sample based on the stoichiometric concentration.

[0063] For example, using the acquisition time as the reference parameter, the end point of the titration is found, which represents the time information. Multiplying this constant speed by the time difference between the titration end and the start of the titration allows calculation of the total volume of sample that flowed from the start to the end of the titration, thus obtaining the sample flow rate. Similarly, the second precision pump for the titrant also operates at a constant speed, and the speed is multiplied by time. If the titrant pump gradually accelerates (e.g., starting slowly and gradually increasing), the total flow rate needs to be calculated using variable-speed flow calculations, thus obtaining the cumulative flow rate of the titrant. Therefore, based on the one-to-one pairing principle, the number of "base molecules" in these titrants equals the number of "acid molecules" in the sample. Finally, dividing the number of "acid molecules" by the total sample flow rate yields the target concentration of the sample.

[0064] For example, a dual-channel peristaltic pump with a flow cell optical path of 10 mm was used, connected to a UV-Vis spectrometer (detection wavelength: 550 nm). Channel one pumped in a diluted fruit juice sample, and channel two pumped in a NaOH standard solution containing phenolphthalein indicator. The two streams were mixed through a T-connector and then flowed into a 2-meter-long coil (0.5 mm inner diameter) for reaction, before flowing into the flow cell for detection. The flow rates of both streams were kept constant. After the system stabilized, absorbance-time data were collected. A transition in absorbance from a low baseline plateau to a high plateau was observed. The inflection point time was recorded, and the total acidity of the fruit juice, i.e., the target concentration of the sample, was calculated based on the cumulative flow rate of NaOH and the sample flow rate at this point.

[0065] For example, during titration, the concentration of the titrant in the mixture is monitored in real time. When the spectral signal changes abruptly, the titration endpoint is found; the titration endpoint represents the optimal matching point where the titrant and the sample react completely.

[0066] For example, it is necessary to consider the actual situation of molecular pairing in chemical reactions (such as one calcium ion requiring two EDTA molecules), make corresponding adjustments before calculation, and then determine the target concentration of the sample to be tested based on the stoichiometric point concentration.

[0067] For example, a syringe pump is used to improve flow rate accuracy, and the spectrometer is equipped with a photodiode array detector. Concentration scanning mode is employed. While maintaining a constant sample flow rate, a series of EDTA standard solutions (all containing SCN) with increasing concentrations are sequentially pumped in. - Indicator). Record the stable absorbance value (480 nm) at each concentration. Plot the "EDTA concentration-absorbance" curve. The curve is S-shaped, and the EDTA concentration corresponding to the inflection point is the stoichiometric concentration. Calculate the Fe³⁺ concentration in the water sample based on this. + The accurate concentration, that is, the target concentration corresponding to the sample to be tested.

[0068] In some embodiments, the mixing device is at least one of a coiled tubing or a static mixer; the spectrometer is at least one of an ultraviolet-visible spectrometer, a fluorescence spectrometer, or a photodiode array detector; the first precision pump is at least one of a syringe pump, a peristaltic pump, or a high-pressure constant current pump; and the second precision pump is at least one of the syringe pump, the peristaltic pump, or the high-pressure constant current pump.

[0069] For example, the first precision pump and the second precision pump are at least one of an injection pump, a peristaltic pump, or a high-pressure constant flow pump. This application does not impose specific limitations and the pump can be selected according to actual needs.

[0070] For example, the mixing device is a coiled pipe or a static mixer with an inner diameter of 0.1-1.0 mm. This application does not impose specific limitations and the device can be selected according to actual needs.

[0071] For example, the spectrometer is at least one of a UV-Vis spectrometer, a fluorescence spectrometer, or a photodiode array detector. This application does not impose specific limitations and the selection can be made according to actual needs.

[0072] This method can continuously change the chemical composition of the mixed fluid by altering the flow rate or concentration of the titrant, thereby inducing dynamic changes in the spectral signal.

[0073] This method can be used for acid-base titration, complexometric titration, redox titration, or precipitation titration. This application does not impose any restrictions, and users can choose according to their actual needs.

[0074] In some embodiments, the system further includes a waste liquid treatment device, and the method further includes: when the mixing state meets the preset conditions, controlling the waste liquid switch of the waste liquid treatment device to be in an open state, so that the mixing result flows into the waste liquid treatment device for waste liquid treatment.

[0075] For example, such as Figure 3 As shown, the titration analysis system 100 based on continuous flow and spectral detection also includes a waste liquid treatment device 109. When the mixing state meets preset conditions (e.g., the spectrometer shows that "sufficient mixing" has been achieved), the system automatically sends an opening command to the solenoid valve or pinch valve of the waste liquid treatment device through a controller (such as a programmable logic controller or a microcontroller), switching the waste liquid switch to the open state, thereby redirecting the liquid flow from the outlet of the micro-flow cell from the return pipeline to the waste liquid collection pipeline. At this time, the fully mixed result (i.e., the completely reacted liquid flow) flows into the waste liquid treatment device through the pipeline under the suction of gravity or the downstream peristaltic pump. In addition, the adsorbent, neutralizing agent, or separation membrane components in the waste liquid treatment device will perform harmless treatment on the chemical substances therein (such as neutralizing acids and alkalis, adsorbing heavy metals or organic solvents) to ensure that the discharged or recycled waste liquid meets safety and environmental protection requirements.

[0076] This system can be directly connected to pipelines in industrial production processes for real-time online monitoring and control of reaction processes.

[0077] Example 1: High-precision determination of total acidity in fruit juice was achieved using a dynamic flow rate scanning method. Ultra-high resolution titration was achieved in continuous flow by finely adjusting the titrant flow rate. Both the first and second precision pumps were high-precision peristaltic pumps (e.g., resolution up to 1 μL / min). The mixing device was a PFA tube with an inner diameter of 2 mm. The flow rate of the sample to be tested, F_S, was set to 1500 μL / min, and the initial flow rate of the titrant, F_T, was set to 500 μL / min. Therefore, the initial total flow rate, F_Total, was 2000 μL / min (2.0 mL / min). The target delay time, t, was set to 4 minutes. The required total dead volume, V_dead = F_Total * t = 2000 μL / min * 4 min = 8000 μL = 8.0 mL. The tube length l = V / (π * r²) = 8000 mm³ / (3.14 * (1mm)²) ≈ 2548 mm = 2.55 m. Therefore, the designed coil length is 2.55 m. This is a very reasonable length and easy to arrange in the equipment. The 2.55-meter tube length also ensures that the system has sufficient delay time for thorough mixing and reaction, while maintaining a compact overall size. This method is ideal for laboratory applications requiring extremely high analytical precision.

[0078] For example, a microflow cell with a 10 mm optical path is connected to a UV-Vis spectrometer (detection wavelength: 550 nm). The flow rate F_S of the diluted juice is set and maintained at 1000 μL / min. The initial flow rate F_T of the NaOH solution (containing phenolphthalein) is set to 500 μL / min. F_T is increased by 100 μL / min in 1-minute increments until it reaches 1100 μL / min. The entire scanning process consists of 6 steps and takes 6 minutes. The spectrometer continuously records the absorbance at 550 nm. The spectral signal is obtained. The NaOH flow rate corresponding to the inflection point of the spectral signal is denoted as F_ep. Assuming F_ep = 800 μL / min, according to the stoichiometric relationship: C_Acid * F_S = C_NaOH * F_ep. C_NaOH represents the concentration of NaOH, and C_Acid represents the target concentration of the sample to be tested. Therefore, the target concentration of the sample to be tested is C_Acid = (C_NaOH * 800μL / min) / 1000μL / min.

[0079] Example 2: Using concentration scanning method to analyze Fe³⁺ in wastewater +Concentration monitoring was performed using a four-channel peristaltic pump and an automatic switching valve. The total dead volume remained at 8.0 mL (2.55 m, 2 mm inner diameter tubing). The wastewater sample flow rate F_Sample and titrant (containing SCN) were then added. - The flow rate F_Carrier of the indicator aqueous solution was set to 1000 μL / min, and the total flow rate F_Total was kept constant at 2000 μL / min. Five different concentrations of EDTA standard solution (e.g., 0.05, 0.10, 0.15, 0.20, 0.25 mmol / L) were sequentially pumped into the system as titrants via an automatic switching valve. Each concentration was pumped for 5 minutes to ensure the signal reached complete stability after a 4-minute delay. The stable absorbance value at 480 nm was recorded for each EDTA concentration. The spectral signal was obtained. The EDTA concentration corresponding to the inflection point of the spectral signal was denoted as C_ep. The target concentration of the test sample was then calculated based on the EDTA concentration corresponding to the inflection point of the curve. Each concentration point was stabilized for 5 minutes, requiring a total of 25 minutes for all five points. This method, due to its constant flow rate, stable pressure, low baseline noise, and excellent reproducibility, is highly suitable for online quality control.

[0080] A titration analysis method based on continuous flow and spectral detection is applied to a titration analysis system based on continuous flow and spectral detection. This system includes at least two precision pumps for delivering the sample stream and titrant stream at constant flow rates, respectively; an inlet line connected to the precision pumps; a mixing device (such as a coil mixer or static mixer) for continuous mixing and chemical reaction of the sample and titrant streams; and a microflow cell and a spectrometer. The microflow cell is located downstream of the fluid transport and mixing device, with its optical window positioned in the optical path of the spectrometer for real-time detection of the spectral signal of the mixture flowing through it. A controller connected to the spectrometer receives and records the spectral signal changing over time, and then correlates the spectral signal with flow rate or time information from the precision pumps; based on the correlated data, the titration endpoint is determined, and the concentration of the sample is calculated.

[0081] For example, the sample and titrant are driven into the flow path at a preset constant flow rate for continuous mixing and reaction. A spectrometer is used to monitor the change in the spectral signal of the reaction stream over time. The spectral signal is correlated with the equivalent volume or concentration parameter of the titrant to identify inflection points or plateau transition points, which correspond to the stoichiometric points of the titration reaction, and the content of the analyte is calculated accordingly.

[0082] Compared with existing technologies, it has the following significant advantages: High throughput and high efficiency: It enables continuous sample injection and detection, and the analysis time for a single sample can be shortened to the second level, greatly improving analytical efficiency; Miniaturization and low consumption: The system flow path is miniaturized, and the consumption of reagents and samples can be reduced to the microliter or even nanoliter level, saving costs and being suitable for micro-analysis; High reproducibility and automation: The entire process is controlled by the instrument, eliminating human operation errors, with excellent reproducibility, and can achieve 24-hour unattended operation; Excellent mixing and mass transfer characteristics: In the continuous flow narrow tube, the mixing efficiency based on laminar diffusion is high and consistent; Powerful online monitoring capability: The system can be directly integrated into the reactor outlet of industrial production processes such as chemical and pharmaceutical industries to achieve real-time, online monitoring of the concentration of key components, providing a powerful tool for process analysis technology.

[0083] Please see Figure 1 , Figure 1 A titration analysis system 100 based on continuous flow and spectral detection is provided in this application embodiment. The system includes at least a first precision pump 101 and a second precision pump 102. The first precision pump 101 is used to deliver the sample to be tested, and the second precision pump 102 is used to deliver the titrant. A mixing device 103 is provided. The first precision pump 101 is connected to the mixing device 103 through a first injection line 1011, and the second precision pump 102 is connected to the mixing device 103 through a second injection line 1021. A light source 104 is provided. Downstream of the mixing device 103, a microflow cell 105, a spectrometer 106, and a reflux device 108 are provided. The system 100 also includes a controller 107, which controls the first precision pump 101 and the second precision pump 102 to respectively deliver the sample to be tested and the titrant. The reagent enters the mixing device 103 to obtain a mixing result; the mixing result is controlled to pass through the micro-flow cell 105 and a spectral signal is obtained using the light source 104 and the spectrometer 106; the mixing result in the mixing device 103 is monitored for sudden changes based on the spectral signal to obtain the mixing state corresponding to the mixing result; when the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell 105 is re-entered into the mixing device 103 through the reflux device 108, and the second precision pump 102 is controlled to continue to deliver the titrant into the mixing device 103 until the mixing state of the re-mixed mixing result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint corresponding to the test sample under the titrant is determined based on the spectral signal; the target concentration corresponding to the test sample is calculated based on the titration endpoint.

[0084] In some implementations, the titration analysis system 100 based on continuous flow and spectral detection can be applied to terminal equipment.

[0085] It should be noted that those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working process of the titration analysis system 100 based on continuous flow and spectral detection described above can be referred to the corresponding process in the aforementioned titration analysis method embodiment based on continuous flow and spectral detection, and will not be repeated here.

[0086] Please see Figure 4 , Figure 4 This is a schematic block diagram of the structure of a terminal device provided in an embodiment of the present invention.

[0087] like Figure 4 As shown, the terminal device 300 includes a processor 301 and a memory 302, which are connected via a bus 303, such as an I2C (Inter-integrated Circuit) bus.

[0088] Specifically, processor 301 provides computing and control capabilities to support the operation of the entire terminal device. Processor 301 can be a Central Processing Unit (CPU), but it can also be other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor.

[0089] Specifically, the memory 302 can be a Flash chip, a read-only memory (ROM) disk, an optical disk, a USB flash drive, or a portable hard drive, etc.

[0090] Those skilled in the art will understand that Figure 4 The structure shown is merely a block diagram of a portion of the structure related to the embodiments of the present invention, and does not constitute a limitation on the terminal device to which the embodiments of the present invention are applied. A specific server may include more or fewer components than those shown in the figure, or combine certain components, or have different component arrangements.

[0091] The processor is used to run a computer program stored in a memory, and when executing the computer program, implements any of the titration analysis methods based on continuous flow and spectral detection provided in the embodiments of the present invention.

[0092] In one embodiment, the processor is configured to run a computer program stored in memory, and when executing the computer program, perform the following steps: A titration analysis system based on continuous flow and spectral detection is provided. The system includes at least a first precision pump and a second precision pump. The first precision pump is used to deliver the sample to be tested, and the second precision pump is used to deliver the titrant. A mixing device is provided, with the first precision pump connected to the mixing device via a first injection line, and the second precision pump connected to the mixing device via a second injection line. A light source is provided, and a microflow cell, a spectrometer, and a reflux device are located downstream of the mixing device. The method includes: The first precision pump and the second precision pump are controlled to respectively transport the sample to be tested and the titrant into the mixing device to obtain the mixing result; The mixing result is controlled to pass through the microflow cell and a spectral signal is obtained using the light source and the spectrometer; Based on the spectral signal, abrupt changes in the mixing result in the mixing device are monitored to obtain the mixing state corresponding to the mixing result; When the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue to deliver the titrant into the mixing device until the mixing state of the re-mixed mixing result meets the preset conditions. When the mixing state meets the preset conditions, the titration endpoint of the sample to be tested under the titrant is determined according to the spectral signal. Calculate the target concentration of the sample to be tested based on the titration endpoint.

[0093] It should be noted that those skilled in the art will understand that, for the sake of convenience and brevity, the specific working process of the terminal equipment described above can be referred to the corresponding process in the aforementioned embodiment of the titration analysis method based on continuous flow and spectral detection, and will not be repeated here.

[0094] This invention also provides a storage medium for computer-readable storage, wherein the storage medium stores one or more programs that can be executed by one or more processors to implement the steps of any of the titration analysis methods based on continuous flow and spectral detection provided in the specification of this invention.

[0095] The storage medium can be an internal storage unit of the terminal device described in the foregoing embodiments, such as the hard drive or memory of the terminal device. Alternatively, the storage medium can be an external storage device of the terminal device, such as a plug-in hard drive, Smart Media Card (SMC), Secure Digital (SD) card, or Flash Card equipped on the terminal device.

[0096] Those skilled in the art will understand that all or some of the steps, systems, or apparatuses disclosed above, and their functional modules / units, can be implemented as software, firmware, hardware, or suitable combinations thereof. In hardware embodiments, the division between functional modules / units mentioned in the above description does not necessarily correspond to the division of physical components; for example, a physical component may have multiple functions, or a function or step may be performed collaboratively by several physical components. Some or all physical components may be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit. Such software may be distributed on a computer-readable medium, which may include computer storage media (or non-transitory media) and communication media (or transient media). As is known to those skilled in the art, the term computer storage media includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data). Computer storage media include, but are not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disc (DVD) or other optical disc storage, magnetic cartridges, magnetic tape, disk storage or other magnetic storage devices, or any other medium that can be used to store desired information and can be accessed by a computer. Furthermore, it is well known to those skilled in the art that communication media typically contain computer-readable instructions, data structures, program modules, or other data in modulated data signals such as carrier waves or other transmission mechanisms, and may include any information delivery medium.

[0097] It should be understood that the term "and / or" as used in this specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, herein, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or system that includes that element.

[0098] The sequence numbers of the above embodiments of the present invention are merely for descriptive purposes and do not represent the superiority or inferiority of the embodiments. The above descriptions are only specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and these modifications or substitutions should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A titration analysis method based on continuous flow and spectral detection, characterized in that, A titration analysis system based on continuous flow and spectral detection is provided. The system includes at least a first precision pump and a second precision pump. The first precision pump is used to deliver the sample to be tested, and the second precision pump is used to deliver the titrant. A mixing device is provided, with the first precision pump connected to the mixing device via a first injection line, and the second precision pump connected to the mixing device via a second injection line. A light source is provided, and a microflow cell, a spectrometer, and a reflux device are located downstream of the mixing device. The method includes: The first precision pump and the second precision pump are controlled to respectively transport the sample to be tested and the titrant into the mixing device to obtain the mixing result; The mixing result is controlled to pass through the microflow cell and a spectral signal is obtained using the light source and the spectrometer; Based on the spectral signal, abrupt changes in the mixing result in the mixing device are monitored to obtain the mixing state corresponding to the mixing result; When the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue to deliver the titrant into the mixing device until the mixing state of the re-mixed mixing result meets the preset conditions. When the mixing state meets the preset conditions, the titration endpoint of the sample to be tested under the titrant is determined according to the spectral signal. Calculate the target concentration of the sample to be tested based on the titration endpoint.

2. The method according to claim 1, characterized in that, The control of the first precision pump and the second precision pump to respectively deliver the sample to be tested and the titrant into the mixing device to obtain the mixing result includes: The first flow rate of the sample to be tested entering the mixing device is controlled by the first precision pump and the first sample inlet line; The second flow rate of the titrant entering the mixing device is controlled by the second precision pump and the second injection line; wherein, both the first flow rate and the second flow rate are constant; When the sample to be tested reaches the first flow rate at the first flow rate, the sampling of the sample to be tested is stopped, and pure water is continued to be injected into the mixing device at the first flow rate through the first precision pump and the first injection pipeline. When the sample to be tested reaches the first flow rate at the first flow rate and the titrant reaches the second flow rate at the second flow rate, air bubbles are added using the first precision pump or the second precision pump to isolate the mixture, and the mixing result of the sample to be tested and the titrant in the mixing device is determined.

3. The method according to claim 1, characterized in that, The control of the first precision pump and the second precision pump to respectively deliver the sample to be tested and the titrant into the mixing device to obtain the mixing result includes: The first flow rate of the sample to be tested entering the mixing device is controlled by the first precision pump and the first sample inlet line, and the first flow rate is kept constant. The second flow rate of the titrant entering the mixing device is controlled by the second precision pump and the second injection line, and the concentration change of the titrant is controlled according to the second flow rate; When the sample to be tested reaches the first flow rate at the first flow rate, the sampling of the sample to be tested is stopped, and pure water is continued to be injected into the mixing device at the first flow rate through the first precision pump and the first injection pipeline. When the sample to be tested reaches the first flow rate at the first flow rate and the titrant reaches the second flow rate at the second flow rate, after adding air bubbles using the first precision pump or the second precision pump for isolation, the mixing result of the sample to be tested and the titrant in the mixing device is determined under the condition of the first flow rate, the second flow rate, and the change in the concentration of the titrant.

4. The method according to claim 1, characterized in that, The method of obtaining spectral signals using the light source and the spectrometer includes: The light source is controlled so that the light emitted by the light source passes through the optical window corresponding to the micro-flow cell along the designed optical path, and the corresponding spectral signal is received by the spectrometer.

5. The method according to claim 1, characterized in that, The step of calculating the target concentration of the sample to be tested based on the titration endpoint includes: When the titration endpoint represents time information, the sample flow rate corresponding to the test sample and the cumulative flow rate corresponding to the titrant are determined based on the first velocity corresponding to the test sample and the second velocity corresponding to the titrant, combined with the titration endpoint. The target concentration of the sample to be tested is calculated based on the sample flow rate and the cumulative flow rate. When the titration endpoint is used to represent the stoichiometric concentration of the titrant, the target concentration of the sample to be tested is determined based on the stoichiometric concentration.

6. The method according to claim 1, characterized in that, The mixing device is at least one of a coiled pipeline and a static mixer; the spectrometer is at least one of an ultraviolet-visible spectrometer, a fluorescence spectrometer, or a photodiode array detector; the first precision pump is at least one of an injection pump, a peristaltic pump, or a high-pressure constant current pump; and the second precision pump is at least one of the injection pump, the peristaltic pump, or the high-pressure constant current pump.

7. The method according to any one of claims 1-6, characterized in that, The system also includes a waste liquid treatment device, and the method further includes: When the mixing state meets the preset conditions, the waste liquid switch of the waste liquid treatment device is turned on, so that the mixing result flows into the waste liquid treatment device for waste liquid treatment.

8. A titration analysis system based on continuous flow and spectral detection, characterized in that, The system includes at least a first precision pump and a second precision pump. The first precision pump is used to deliver the sample to be tested, and the second precision pump is used to deliver the titrant. A mixing device is included, with the first precision pump connected to the mixing device via a first injection line, and the second precision pump connected to the mixing device via a second injection line. A light source is provided, and a microflow cell, a spectrometer, and a reflux device are located downstream of the mixing device. The system also includes a controller, which performs the following actions: The first and second precision pumps are controlled to transport the sample to be tested and the titrant into the mixing device to obtain a mixing result; the mixing result is controlled to pass through the micro flow cell and a spectral signal is obtained using the light source and the spectrometer. The mixing state corresponding to the mixing result is obtained by abruptly monitoring the mixing result in the mixing device according to the spectral signal; when the mixing state does not meet the preset conditions, the mixing result in the micro-flow cell is re-flowed into the mixing device through the reflux device, and the second precision pump is controlled to continue to deliver the titrant into the mixing device until the mixing state of the re-mixed mixing result meets the preset conditions; when the mixing state meets the preset conditions, the titration endpoint corresponding to the test sample under the titrant is determined according to the spectral signal. Calculate the target concentration of the sample to be tested based on the titration endpoint.

9. A terminal device, characterized in that, The terminal device includes a processor and a memory; The memory is used to store computer programs; The processor is used to execute the computer program and, in executing the computer program, implement a titration analysis method based on continuous flow and spectral detection as described in any one of claims 1 to 7.

10. A computer storage medium for computer storage, characterized in that, The computer storage medium stores one or more programs, which can be executed by one or more processors to implement the steps of the titration analysis method based on continuous flow and spectral detection according to any one of claims 1 to 7.

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