Sample testing device and sample testing method

Abstract

The application discloses a sample detection device and a sample detection method. The first detection module of the sample detection device is used for detecting a sample in a glycation detection item; the second detection module is used for detecting the sample in other detection items; and the processor is used for controlling the liquid path support module to clean the first detection module when the second detection module detects the sample, so that the first detection module is switched to a dormant state or is woken up from the dormant state. When the second detection module detects the sample, the sample detection device switches the first detection module to the dormant state or wakes up the first detection module, so that the sample detection device can control the first detection module to be in the dormant state when the glycation detection item is not detected, and control the first detection module to be woken up from the dormant state when the glycation detection item is detected, thereby reducing the consumption of reagents and power caused by the first detection module maintaining detection pressure, reducing detection cost and improving the use rate of glycation reagents.

Description

Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to sample testing devices and sample testing methods. Background Technology

[0002] When performing saccharification testing, existing sample testing devices require a pressure build-up process after each batch of tests is started. This process ensures that the high-pressure liquid circuit in the first testing module of the sample testing device reaches and maintains the required pressure for testing. Only under this pressure will the first testing module perform saccharification testing on the sample.

[0003] When the sample detection device includes a second detection module that can perform other detection items, the detection items of the injected samples differ. Some samples need to be tested for saccharification, while others do not. The first detection module requires a lot of time for the cleaning and pressure building processes. In the case of joint detection or mixed detection, the existing instruments will keep the first detection module in the aforementioned pressure maintenance state to maintain the detection pressure of the first detection module, which leads to the continuous consumption of eluent and waste of saccharification reagent. Summary of the Invention

[0004] To address the aforementioned technical problems, this application provides a sample detection device and a sample detection method.

[0005] To address the aforementioned problems, this application provides a first technical solution: a sample detection device comprising a sample injection module, a first detection module, a second detection module, a liquid path support module, and a processor; the sample injection module is used to receive a sample to be tested; the first detection module is connected to the sample injection module and is used to perform saccharification detection on the sample to be tested; the second detection module is connected to the sample injection module and is used to perform other detection items on the sample to be tested besides saccharification detection; the liquid path support module is connected to the first detection module; the processor is connected to the sample injection module, the first detection module, and the second detection module respectively, and the processor is used to control the liquid path support module to clean the first detection module when controlling the second detection module to detect the sample, so that the first detection module switches to a dormant state or wakes up from the dormant state.

[0006] To address the aforementioned issues, this application provides a second technical solution: a sample detection method applied to the sample detection device described above, the sample detection method comprising: receiving a sample to be detected; and cleaning the first detection module of the sample detection device while the second detection module of the sample detection device is performing detection on the sample for items other than saccharification detection, so as to switch the first detection module to the dormant state or wake it up from the dormant state.

[0007] Optionally, when the second detection module of the sample detection device performs tests on the sample other than the saccharification test, the first detection module of the sample detection device is cleaned. This includes: when the sample injection module of the sample detection device is in automatic injection mode, the second detection module is detecting the sample, and the first detection module is in the dormant state, if the first sample including the saccharification test is received, the first detection module is cleaned and pressure-built, and the first detection module is controlled to perform saccharification test on the sample including the saccharification test.

[0008] Optionally, the cleaning of the first detection module of the sample detection device to switch the first detection module to a dormant state or wake it up from the dormant state includes: acquiring the detection items of multiple samples to be tested; when the detection items of the multiple samples include the saccharification detection item, acquiring the interval time between the first sample including the saccharification detection item and the current detection position; when the interval time is less than or equal to a first preset time, performing a cleaning operation or a cleaning pressure build-up operation on the first detection module to wake it up from the dormant state.

[0009] Optionally, the cleaning of the first detection module of the sample detection device to switch the first detection module to a dormant state or wake it up from the dormant state includes: in response to the triggering of the dormant wake-up button of the first detection module, performing a cleaning operation on the first detection module to wake it up from the dormant state; when the sample injection module receives a sample including the saccharification detection item, performing a pressure build-up operation on the first detection module and controlling the first detection module to perform saccharification detection on the sample including the saccharification detection item.

[0010] Optionally, the above sample detection method includes: when the first detection module is in a pressure maintenance state, upon receiving a sleep command from the first detection module, controlling the first detection module to continue maintaining the pressure maintenance state for a second preset time; if a sample including the saccharification detection item is received within the second preset time, controlling the first detection module to detect the sample including the saccharification detection item; if no sample including the saccharification detection item is received within the second preset time, cleaning the first detection module to switch the first detection module to the sleep state.

[0011] Optionally, controlling the first detection module to continue maintaining the pressure maintenance state for a second preset time includes: controlling the liquid path support module to continuously supply eluent to the detection pipeline of the first detection module so that the first detection module is maintained in the pressure maintenance state; wherein, the range of the second preset time is the time range of the first volume of eluent consumed by the detection pipeline, and the first volume of eluent is less than or equal to the second volume of eluent used by the first detection module to complete one saccharification detection item.

[0012] Optionally, the above sample detection method includes: generating the above-mentioned sleep command when the automatic sample injection mode of the sample injection module of the above-mentioned sample detection device ends; or, generating the above-mentioned sleep command when the current sample detection obtained by the above-mentioned sample detection device through closed sample injection mode or open sample injection mode is completed; or, generating the above-mentioned sleep command when the sample injection end button of the sample injection module of the above-mentioned sample detection device is triggered; or, based on the detection items of multiple samples to be detected, if the detection items of the multiple samples include the above-mentioned saccharification detection item, generating the above-mentioned sleep command when the detection of the last sample including the above-mentioned saccharification detection item is completed.

[0013] Optionally, the above sample detection method further includes: in response to a first operation command for the sample detection device, cleaning the second detection module to switch the second detection module to a preset operation state, and controlling the first detection module to remain in the sleep state; wherein the first operation command is used to instruct the second detection module to switch to the preset operation state, and the preset operation state includes at least one of a soft-power-on state, a soft-power-off state, the sleep state, and waking up from the sleep state.

[0014] Optionally, the above sample detection method further includes: cleaning the first detection module and the second detection module in response to a second operation command for the sample detection device, wherein the second operation command is used to instruct the sample detection device to perform a hard boot.

[0015] This application provides a sample detection device and a sample detection method. The sample detection device has an injection module for receiving the sample to be tested; a first detection module connected to the injection module for performing saccharification detection on the sample; a second detection module connected to the injection module for performing other detections on the sample besides saccharification detection; a liquid path support module connected to the first detection module; and a processor connected to the injection module, the first detection module, and the second detection module. The processor controls the liquid path support module to clean the first detection module while controlling the second detection module to detect the sample, thereby switching the first detection module to a dormant state or waking it up from a dormant state. Through this method, the sample detection device of this application can switch the first detection module to a dormant state or wake it up from a dormant state while the second detection module is detecting the sample. This allows the sample detection device to control the first detection module to be in a dormant state when saccharification detection is not required, and to control the first detection module to wake it up from a dormant state when saccharification detection is required. This reduces the consumption of reagents and electricity by the first detection module in maintaining detection pressure, lowers detection costs, and increases the utilization rate of saccharification reagents. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0017] Figure 1 This is a schematic diagram of the structure of the first embodiment of the sample detection device provided in this application;

[0018] Figure 2 This is a schematic diagram of the structure of the second embodiment of the sample detection device provided in this application;

[0019] Figure 3 This is a flowchart illustrating the first embodiment of the sample detection method provided in this application;

[0020] Figure 4 This is a flowchart illustrating the second embodiment of the sample detection method provided in this application;

[0021] Figure 5 This is a flowchart illustrating the third embodiment of the sample detection method provided in this application;

[0022] Figure 6 This is a schematic diagram of the structure of an embodiment of the computer-readable storage medium provided in this application;

[0023] Among them, 11 is the sample injection module; 12 is the first detection module; 13 is the second detection module; 14 is the liquid path support module; and 15 is the processor. Detailed Implementation

[0024] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0025] It should be noted that if the embodiments of this application involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0026] Furthermore, if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0027] Please see Figure 1 and Figure 2 , Figure 1 This is a schematic diagram of the structure of the first embodiment of the sample detection device provided in this application. Figure 2 This is a schematic diagram of the second embodiment of the sample detection device provided in this application. Figure 1 and Figure 2 As shown, the sample detection device provided in this embodiment includes a sample injection module 11, a first detection module 12, a second detection module 13, a liquid path support module 14, and a processor 15.

[0028] The sample injection module 11 is used to receive the sample to be tested; the first detection module 12 is connected to the sample injection module 11 and is used to perform saccharification detection on the sample to be tested; the second detection module 13 is connected to the sample injection module 11 and is used to perform other detection items on the sample to be tested besides saccharification detection; the liquid path support module 14 is connected to the first detection module 12; the processor 15 is connected to the sample injection module 11, the first detection module 12 and the second detection module 13 respectively, and the processor 15 is used to control the liquid path support module 14 to clean the first detection module 12 when controlling the second detection module 13 to detect the sample, so that the first detection module 12 switches to a dormant state or wakes up from the dormant state.

[0029] Specifically, the sample introduction module 11 may include a sampler and a sampling holder. The sample to be tested is stored in a sample tube, the sampling holder is used to hold the sample tube, and the sampler is positioned above the sampling holder to aspirate the sample from the sample tube. The sample introduction module 11 can be connected to the first detection module 12 via tubing, so that the first detection module 12 receives the sample collected by the sample introduction module 11 and performs glycation assay on the sample; and / or, the sample introduction module 11 can be connected to the second detection module 13 via tubing, so that the second detection module 13 receives the sample collected by the sample introduction module 11 and performs assays on the sample other than glycation assays. In possible embodiments, other assays performed by the second detection module 13 include, but are not limited to, routine blood count assays, specific protein assays, reticulocyte assays, and differential white blood cell counts.

[0030] The first detection module 12 uses high-performance liquid chromatography (HPLC) to detect glycated hemoglobin in the sample. Specifically, the first detection module 12 uses eluent and a high-pressure pump to maintain high-pressure infusion. The eluent may include salt solutions of different concentrations. Under a preset detection pressure, the eluent in the detection pipeline elutes different components of hemoglobin through an ion exchange column. The stationary phase in the ion exchange column carries an electric charge and interacts with the charge on the hemoglobin, achieving separation of glycated hemoglobin from the sample solution based on the charge difference. The first detection module 12 may also include a detector, which is located in the detection pipeline after the ion exchange column. The detector is used to measure the absorbance of the separated glycated hemoglobin to calculate the concentration of glycated hemoglobin, i.e., the detection result of the glycated hemoglobin test, based on the absorbance of each component. When the sample detection device does not receive a new sample containing glycated hemoglobin, the first detection module 12 needs to maintain its current detection capacity. Therefore, the reagent assembly continuously supplies eluent to maintain the detection pressure in the detection pipeline, allowing the first detection module 12 to maintain a pressure maintenance state for a first preset time.

[0031] In other embodiments, the first detection module 12 may also detect glycated hemoglobin molecules by immunochromatography, liquid-solid chromatography, etc., without specific limitations.

[0032] The processor 15 controls the detection nodes and processes of the sample introduction module 11, the first detection module 12, and the second detection module 13. Furthermore, the first detection module 12 and the second detection module 13 can include a detection state and a sleep state. In the detection state, the processor 15 controls the first detection module 12 and the second detection module 13 to execute the conventional detection process; in the sleep state, the processor 15 controls the liquid path structure and detection devices of the first detection module 12 and the second detection module 13 to stop operating or be in an idle state. When the processor 15 controls the second detection module 13 to detect the sample, the second detection module 13 is in a non-sleep state, at which time the second detection module 13 can perform one or more of the following operation nodes: cleaning, sample aspiration, reaction, and detection. The processes for the first detection module 12 and the second detection module 13 to enter a dormant state can be executed independently. That is, the processor 15 is also used to control one of the first detection module 12 and the second detection module 13 to perform detection, and to control the other of the first detection module 12 and the second detection module 13 to enter a dormant state. This allows the sample detection device to control the detection module to enter a dormant state when a certain detection item is not required, thereby reducing the consumption of reagents, electricity, etc., by the first detection module due to detection pressure. Specifically, when controlling the second detection module 13 to detect the sample, the processor 15 is used to control the liquid path support module 14 to clean the first detection module 12, so that the first detection module 12 switches to a dormant state or wakes up from a dormant state. In some embodiments, the processor 15 can also be used to control the liquid path support module 14 to clean and pressurize the first detection module 12 when controlling the second detection module 13 to detect the sample, so that the first detection module 12 switches to a dormant state or wakes up from a dormant state.

[0033] In this embodiment, the sample detection device has an injection module 11 for receiving the sample to be tested; a first detection module 12 for performing saccharification detection on the sample; a second detection module 13 for performing other detections on the sample besides saccharification detection; a liquid path support module 14 connected to the first detection module 12; and a processor 15 for controlling the liquid path support module 14 to clean the first detection module 12 when the second detection module 13 is detecting the sample, so that the first detection module 12 switches to a dormant state or wakes up from a dormant state. Through this method, the sample detection device of this embodiment can switch the first detection module 12 to a dormant state or wake it up from a dormant state when the second detection module 13 is detecting the sample. This allows the sample detection device to control the first detection module 12 to be dormant when saccharification detection is not required, and to control the first detection module 12 to wake up from a dormant state when saccharification detection is required. This reduces the consumption of reagents, electricity, etc., by the first detection module in maintaining detection pressure, lowers detection costs, and increases the utilization rate of saccharification reagents.

[0034] Please see Figure 3 , Figure 3 This is a flowchart illustrating the first embodiment of the sample detection method provided in this application. Figure 3 As shown, the sample detection method of this embodiment is applied to the sample detection device of any of the above embodiments. The sample detection method of this embodiment includes the following steps:

[0035] Step S11: Receive the sample to be tested.

[0036] Upon receiving a sample to be tested, the sample information can be obtained by scanning the sample. The sample information indicates the test items that need to be tested for the sample, so that the second test module 13 and / or the first test module 12 can perform the corresponding test items on the sample.

[0037] Step S 12: When the second detection module 13 of the sample detection device performs tests on the sample other than the saccharification test, the first detection module 12 of the sample detection device is cleaned so that the first detection module 12 is switched to a dormant state or woken up from the dormant state.

[0038] Specifically, when the sample information corresponding to the sample to be tested indicates that the sample needs to undergo testing for items other than saccharification, the second detection module 13 of the sample detection device controls the sample to perform testing for items other than saccharification. At this time, it indicates that the other detection modules of the sample detection device besides the first detection module 12 are in working state, that is, in non-dormant state, and the first detection module 12 of the sample detection device can be cleaned to switch the first detection module 12 to dormant state or wake it up from dormant state.

[0039] In this embodiment, the sample detection method receives the sample to be tested. While the second detection module 13 of the sample detection device performs tests on the sample other than saccharification detection, the first detection module 12 of the sample detection device is cleaned. This allows the first detection module 12 to switch to a dormant state or wake up from a dormant state. This enables the first detection module 12 to be controlled to be dormant when saccharification detection is not required, and to be woken up from a dormant state when saccharification detection is required. This reduces the consumption of reagents, electricity, etc., by the first detection module 12 in maintaining detection pressure, thereby reducing detection costs and increasing the utilization rate of saccharification reagents.

[0040] In one embodiment, step S12 includes: when the sample injection module 11 of the sample detection device is in automatic sample injection mode, the second detection module 13 detects the sample, and the first detection module 12 is kept in a dormant state, if the first sample including the saccharification detection item is received, the first detection module 12 is cleaned and pressure built up, and the first detection module 12 is controlled to perform saccharification detection on the sample including the saccharification detection item.

[0041] Specifically, the sample introduction module 11 may include an automatic sample introduction component, an open sample introduction component, and a closed sample introduction component. The automatic sample introduction component is equipped with an automatic sample loading area, a scanning mechanism, a mixing mechanism, and a sampler. When the sample introduction module 11 is in automatic sample introduction mode, the user places the sample carried by the test tube rack into the loading area of ​​the automatic sample introduction device. The scanning mechanism scans the sample in the loading area of ​​the automatic sample introduction device, the mixing mechanism mixes the scanned sample, and the sampler aspirates the mixed sample. In automatic sample introduction mode, the second detection module 13 is in a non-dormant state, and the first detection module 12 remains in a dormant state. When the sample introduction module 11 receives sample information indicating that the sample needs to undergo saccharification detection, i.e., when it receives the first sample including saccharification detection, a cleaning and pressurization operation can be performed on the first detection module 12 to wake it up from the dormant state and control the first detection module 12 to perform saccharification detection on the sample including saccharification detection.

[0042] The purpose of cleaning the first detection module 12 is to maintain its cleanliness, reduce the risk of cross-contamination during sample testing, and mitigate the impact of static liquid lines on testing during dormancy. The purpose of pressurizing the first detection module 12 after cleaning is to maintain the high-pressure liquid line section within the first detection module 12 at the required pressure for testing, so that the first detection module 12 can perform saccharification testing on samples including those requiring saccharification testing.

[0043] In this embodiment of the application, the sample detection method involves the sample injection module 11 of the sample detection device being in automatic injection mode, the second detection module 13 detecting the sample, and the first detection module 12 remaining in a dormant state. If the first sample including the saccharification detection item is received, the first detection module 12 is cleaned and pressure-built, and the first detection module 12 is controlled to perform saccharification detection on the sample including the saccharification detection item. This allows the dormant first detection module 12 to be automatically awakened from its dormant state when saccharification detection is required, reducing the consumption of reagents, electricity, etc., caused by the first detection module 12 waiting for detection and maintaining detection pressure for a long time, thereby reducing detection costs and increasing the utilization rate of saccharification reagents.

[0044] Furthermore, the processor 15 of the sample detection device can perform the above-mentioned steps when the sample injection module 11 of the sample detection device is in automatic sample injection mode, the second detection module 13 detects the sample, and the first detection module 12 is kept in a dormant state. If the first sample including the saccharification detection item is received, the processor 15 performs a cleaning and pressure building operation on the first detection module 12 and controls the first detection module 12 to perform saccharification detection on the sample including the saccharification detection item. This allows the dormant wake-up of the first detection module 12 to be separately controlled from the second detection module 13.

[0045] In one embodiment, please refer to Figure 4 , Figure 4 This is a flowchart illustrating the second embodiment of the sample detection method provided in this application. Figure 4 As shown, step S12 includes:

[0046] Step S21: Obtain the detection items for multiple samples to be tested.

[0047] Specifically, the test items for multiple samples to be tested can be obtained through batch sample information scanning. For example, the user places a batch of samples on the automatic sample loading area of ​​the automatic sample loading component, so that the scanning mechanism scans the multiple samples sequentially and obtains the test items for multiple samples from the sample information. The test items for multiple samples to be tested can also be obtained from sample information imported by the user, or the test items for multiple samples can be directly input or set by the user; no specific limitation is made here. Optionally, when the sample testing device is in a production line or connected to a laboratory management system, the test items for multiple samples to be tested can also be obtained directly from the production line management terminal or the laboratory management system.

[0048] Step S22: When the detection items for multiple samples include the saccharification detection item, obtain the time interval between the first sample that includes the saccharification detection item and the current detection position.

[0049] When multiple samples are tested, including those for glycosylation detection, the testing order of the samples can be determined based on their arrangement and information. Furthermore, the time interval between the first sample containing glycosylation detection and the current testing position can be obtained based on the testing time of each sample. Here, the current testing position is the testing position of the first testing module 12; the time interval between the first sample containing glycosylation detection and the current testing position can be understood as the waiting time for the first sample containing glycosylation detection in the determined testing order to reach the testing position of the first testing module 12 from the waiting sequence.

[0050] Step S23: When the interval time is less than or equal to the first preset time, perform a cleaning operation or a cleaning pressure build-up operation on the first detection module 12 to wake up the first detection module 12 from the hibernation state.

[0051] After determining the time interval between the first sample including the glycation detection item and the current detection position, if the interval is less than or equal to a first preset time, a cleaning operation or a cleaning and pressure building operation is performed on the first detection module 12 to wake it up from its dormant state. The first preset time can be the time required to wake up the first detection module 12. If the interval is less than or equal to the first preset time, meaning the time it takes for the first sample including the glycation detection item to reach the detection position of the first detection module 12 is less than or equal to the time required to wake up the first detection module 12, the first detection module 12 can be woken up earlier. This reduces the time the first sample including the glycation detection item waits for the first detection module 12 to wake up from its dormant state, thus accelerating the time for the first sample including the glycation detection item to complete the detection and obtain the detection result.

[0052] The cleaning operation refers to cleaning the first detection module 12 at least once. The cleaning operation includes, but is not limited to, at least one operation such as cleaning the seals on the first detection module 12, cleaning the connecting pipes of the first detection module 12, and removing residual liquid and air bubbles from the connecting pipes.

[0053] The cleaning and pressure building operation involves cleaning the first detection module 12 and then directly pressurizing it to maintain the high-pressure liquid circuit of the first detection module 12 at the required pressure for saccharification detection. Alternatively, the cleaning and pressure building operation can involve cleaning the first detection module 12 and then waiting for a period of time or waiting until the first detection module 12 needs to perform saccharification detection before pressurizing it. This reduces the consumption of reagents, electricity, etc., when maintaining the high-pressure liquid circuit of the first detection module 12 at the required pressure for detection, and improves the utilization rate of saccharification reagents.

[0054] In the above manner, the sample detection method obtains the detection items of multiple samples to be detected; when the detection items of multiple samples include the saccharification detection item, it obtains the interval time between the first sample including the saccharification detection item and the current detection position; when the interval time is less than or equal to a first preset time, it performs a cleaning operation or a cleaning pressure build-up operation on the first detection module 12 to wake up the first detection module 12 from the dormant state.

[0055] Furthermore, the liquid path support module 14 may include a cleaning solution storage component, a reagent component, and a liquid path control component. The cleaning solution storage component can be connected to the first detection module 12 via a pipeline. The liquid path control component is used to control the connection between the cleaning solution storage component and the first detection module 12 in a certain state, so that the cleaning solution in the cleaning solution storage component flows to the first detection module 12 and performs at least one cleaning operation on the first detection module 12. The reagent component is used to store the eluent, and the liquid path support module 14 is used to continuously supply the eluent to the first detection module 12 so that the first detection module 12 can perform a pressure build-up operation. The processor 15 of the sample detection device can control the corresponding actions of the liquid path support module 14 to execute the above steps S21-S23, thereby controlling the first detection module 12 to wake up from its dormant state based on the interval between the first sample including the saccharification detection item and the current detection position.

[0056] In one embodiment, step S12 includes: in response to the triggering of the sleep wake-up button of the first detection module 12, performing a cleaning operation on the first detection module 12 to wake up the first detection module 12 from the sleep state; when the sample injection module 11 receives a sample including the saccharification detection item, performing a pressure build-up operation on the first detection module 12 and controlling the first detection module 12 to perform saccharification detection on the sample including the saccharification detection item.

[0057] Specifically, the sample detection method of this embodiment can also wake up the first detection module 12 from its sleep state by being triggered by the user's sleep wake-up button. The sleep wake-up button can be a mechanical button located on the front cover of the sample detection device, which the user activates to wake it up; or, the sleep wake-up button can be a software button on the operating program connected to the sample detection device, which the user activates on the operating program or interface to wake it up.

[0058] Understandably, in this embodiment, when the sleep / wake-up button is triggered, the first detection module 12 is first cleaned so that it can be prepared for wake-up; and when the sample injection module 11 receives a sample including the saccharification detection item, the first detection module 12 is pressure-built to reduce the consumption of reagents, electricity, etc. caused by maintaining pressure while the first detection module 12 is waiting for the sample including the saccharification detection item, thereby further improving the utilization rate of saccharification reagent.

[0059] For example, the sample detection method of this embodiment can be triggered by the sleep wake-up button when the second detection module 13 is in the detection / working mode, that is, in the non-sleep state; the sleep wake-up button of this embodiment can also be triggered when the entire sample detection device is powered on, that is, when the second detection module 13 is in the sleep state or the power-off mode, the sleep wake-up of the first detection module 12 is started at the same time. Here, the sleep wake-up scenario of this embodiment is not specifically limited.

[0060] The processor 15 of the sample detection device can be triggered by executing the sleep wake-up button of the first detection module 12 to perform a cleaning operation on the first detection module 12, so as to wake up the first detection module 12 from the sleep state. When the sample injection module 11 receives a sample including the saccharification detection item, it performs a pressure build-up operation on the first detection module 12 and controls the first detection module 12 to perform saccharification detection on the sample including the saccharification detection item, so as to reduce the consumption of reagents, electricity, etc. by the first detection module 12 in maintaining pressure while waiting for the sample including the saccharification detection item.

[0061] In one embodiment, please refer to Figure 5 , Figure 5 This is a flowchart illustrating the third embodiment of the sample detection method provided in this application. Figure 5 As shown, the sample detection method also includes:

[0062] Step S31: When the first detection module 12 is in the pressure maintenance state, upon receiving a sleep command from the first detection module 12, control the first detection module 12 to continue maintaining the pressure maintenance state for a second preset time.

[0063] When the first detection module 12 is in a pressure maintenance state, it can be understood that the first detection module 12 is ready to perform saccharification detection at any time, i.e., the first detection module 12 is in normal detection mode. At this time, upon receiving a sleep command from the first detection module 12, the system controls the first detection module 12 to continue maintaining the pressure maintenance state for a second preset time. During the process of the first detection module 12 maintaining the pressure maintenance state for the second preset time, if a sample including saccharification detection items is received, the process proceeds to step S32; if no sample including saccharification detection items is received, the process proceeds to step S33.

[0064] Step S32: If a sample including the saccharification detection item is received within the second preset time, the first detection module 12 is controlled to detect the sample including the saccharification detection item.

[0065] When a sample including a saccharification test item is received within a second preset time, the first detection module 12 can be controlled to detect the sample including the saccharification test item. Therefore, the sample detection method of this embodiment can maintain a pressure maintenance state for a period of time after receiving a sleep command, so that when a sample including a saccharification test item is put on the machine within the second preset time, it can be detected quickly, avoiding or reducing the frequent switching between sleep state and wake-up state of the first detection module 12 due to short sample loading time, thereby improving the reliability of the first detection module 12.

[0066] Step S33: If no sample including the saccharification detection item is received within the second preset time, the first detection module 12 is cleaned so that the first detection module 12 is switched to a dormant state.

[0067] If no sample including the saccharification test is received within the second preset time, the first detection module 12 can be cleaned directly to switch the first detection module 12 to a dormant state, thereby reducing the consumption of reagents, electricity, etc. caused by maintaining pressure in the dormant state.

[0068] The processor 15 of the sample detection device can execute the above steps S21-S23 when it receives the sleep command of the first detection module 12, so that the first detection module 12 maintains the pressure maintenance state for a period of time after receiving the sleep command.

[0069] Optionally, step S31 includes: controlling the liquid path support module 14 to continuously provide eluent to the detection pipeline of the first detection module 12 so that the first detection module 12 is maintained in a pressure maintenance state; wherein, the range of the second preset time is the time range of the first volume of eluent consumed by the detection pipeline, and the first volume of eluent is less than or equal to the second volume of the first detection module 12 to complete one saccharification detection item.

[0070] Specifically, the first detection module 12 includes a detection line, and a liquid support module 14 is connected to the detection line. The reagent component of the liquid support module 14 stores eluent. This sample detection method controls the liquid support module 14 to continuously supply eluent to the detection line, so that the first detection module 12 maintains a pressure maintenance state for a second preset time. The second preset time is the time range of the first volume of eluent consumed by the detection line, and the first volume of eluent is less than or equal to the second volume of eluent used by the first detection module 12 to complete one saccharification detection item.

[0071] Specifically, the volume of eluent required by the first detection module 12 to complete one glycation test is defined as the second volume, and the volume of eluent required by the first detection module 12 to maintain the pressure for a second preset time is defined as the first volume. The first volume is less than or equal to the second volume. The eluent may include salt solutions of different concentrations. Under a preset detection pressure, the eluent in the detection pipeline performs gradient elution of different components of hemoglobin through an ion exchange column. The stationary phase in the ion exchange column carries a charge and can interact with the charge on the hemoglobin, achieving separation of hemoglobin from the sample solution based on the charge difference. The first detection module 12 may also include a detector, which is installed in the detection pipeline. The detector is used to measure the absorbance of the separated hemoglobin to calculate the concentration of hemoglobin based on the absorbance of each component. When the sample detection device does not receive a new sample including the glycation test, since the first detection module 12 still needs to maintain its current detection capacity, it needs to continuously supply eluent through the reagent assembly to maintain the detection pressure of the detection pipeline, so that the first detection module 12 can maintain the pressure for a first preset time.

[0072] In a possible implementation, the first liquid volume can be 0.15 to 0.9 times the second liquid volume. For example, the first liquid volume can be 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, or 0.9 times the second liquid volume. Understandably, before switching the first detection module 12 to a dormant state, the pressure of the first detection module 12 is maintained for a second preset time period. If no new samples including saccharification detection items are processed within the second preset time period, the reagent cost is within an acceptable range. If samples including saccharification detection items are processed within the second preset time period, unlike the scheme that directly enters dormancy after receiving a sample injection end command, the scheme of this embodiment can directly start sample detection under the pressure maintenance state within the second preset time period. The saved reagent cost includes the difference between the reagent cost of waking up from the dormant state and the reagent cost of the first liquid volume. For example, when the first liquid volume is 0.45 times the second liquid volume, if a sample including the saccharification detection item is fed into the machine within the second preset time, the saved reagent cost can correspond to 2 to 3 times the second liquid volume, that is, the reagent cost saved by the first detection module 1212 in completing the saccharification detection item of 2 to 3 samples.

[0073] In this embodiment of the application, the sample detection method sets the second preset time for maintaining the first detection module 12 in the pressure maintenance state as the time range for detecting the first volume of eluent consumed by the detection pipeline. The first volume of eluent is less than or equal to the second volume for the first detection module 12 to complete one saccharification detection item. This allows the sample detection method to directly start saccharification detection when a sample including saccharification detection item is loaded within the second preset time, reducing or avoiding reagent loss during frequent dormancy and wake-up processes caused by short-term sample loading, reducing reagent costs from waking up from dormancy, and further improving the utilization rate of saccharification reagents.

[0074] The first detection module 12 may further include a sample preparation line, which is connected to both the sample injection module 11 and the detection line. The sample preparation line is used to acquire a sample and dilute and hemolyze it to obtain the sample solution to be tested. The detection line is used to elute the sample solution multiple times with an elution buffer under pressure maintenance to achieve the elution and separation of glycated hemoglobin. The processor 15 of the sample detection device can control the amount of elution buffer used by the liquid path support module 14 when delaying the first detection module 12 for a second preset time, so that the first detection module 12 maintains a pressure maintenance state for a delayed period after receiving a sleep command.

[0075] In other embodiments, the second preset time ranges from 0.5 min to 3 min.

[0076] Specifically, the second preset time can be 0.5, 0.75, 1, 1.25, 1.5, 1.75, 2, 2.25, 2.5, 2.75, or 3 minutes. Setting the second preset time within the range of 0.5 minutes to 3 minutes allows for direct sample detection when samples including those for saccharification testing are available within this timeframe. This reduces the impact of the first detection module 12's dormancy on the sample detection efficiency for saccharification testing, thereby further reducing reagent costs associated with maintaining the pressure during the second preset time while ensuring sample detection efficiency.

[0077] Optionally, the sample detection method includes: generating a sleep command when the automatic sample injection mode of the sample injection module 11 of the sample detection device ends; or, generating a sleep command when the current sample detection acquired by the sample detection device through closed sample injection mode or open sample injection mode is completed; or, generating a sleep command when the sample injection end button of the sample injection module 11 of the sample detection device is triggered; or, based on the detection items of multiple samples to be detected, if the detection items of multiple samples include saccharification detection items, generating a sleep command when the detection of the last sample including saccharification detection items is completed.

[0078] Specifically, in one embodiment, when the sample injection module 11 of the sample detection device is in automatic injection mode, the automatic injection mode can end when the sample injection process of the automatic injection component ends or the current batch of samples of the automatic injection component is detected. At this time, a sleep command can be generated so that the first detection module 12 switches to sleep state based on the sleep command.

[0079] In another embodiment, the sample introduction module 11 may further include an open sample introduction component and a closed sample introduction component. The open sample introduction component is provided with an open sampling position, which is used to move the sampling needle to the open sampling position. After the user performs manual mixing of the sample and removes the test tube cap, the user moves the test tube to the open sampling position and aligns it with the sampling needle, which is used to penetrate below the liquid surface of the sample to trigger the sampling operation. The closed sample introduction component is provided with a closed sample introduction chamber, which is used to open the closed sample introduction chamber so that the user can place the manually mixed sample on the test tube position in the closed sample introduction chamber. After the closed sample introduction chamber is closed, the closed sample introduction component is used to puncture and aspirate the sample in the closed sample introduction chamber through the sampling needle. In either the closed sample introduction mode or the open sample introduction mode, the sample detection device only needs to control the sampling needle to aspirate the sample for detection. Therefore, when the current sample detection obtained by the sample detection device through the closed sample introduction mode or the open sample introduction mode is completed, a sleep command can be generated so that the first detection module 12 switches to a sleep state based on the sleep command.

[0080] Understandably, the above method puts the sample into hibernation mode when the automatic injection mode ends, or when the sample detection in the closed or open injection mode is completed. Hibernation can be performed according to different injection modes, making the method of this embodiment applicable to multiple injection modes and able to automatically put into hibernation mode under different injection modes. This reduces user operations and also reduces the consumption of reagents, electricity, etc., by the first detection module 12 due to long waiting time for detection and maintaining detection pressure, thereby reducing detection costs and increasing the utilization rate of saccharification reagents.

[0081] In another embodiment, after obtaining the detection items of multiple samples in a batch through the automatic sample introduction component, a dormant command is generated when the detection of the last sample among the multiple samples, which includes the saccharification detection item, is completed, so that the first detection module 12 switches to a dormant state based on the dormant command.

[0082] Specifically, the sample detection device can determine whether multiple samples include the saccharification detection item and the position of the last sample including the saccharification detection item by using the sample information obtained when the sample is scanned by the automatic sample introduction component. Therefore, the method of this embodiment can automatically switch the first detection module 12 to a dormant state when the detection of the last sample including the saccharification detection item is completed. This reduces user operation and also reduces the consumption of reagents, electricity, etc., by the first detection module 12 due to long waiting time for detection and maintaining detection pressure, thereby reducing detection costs and increasing the utilization rate of saccharification reagents.

[0083] Understandably, the three implementation methods described above can be applied simultaneously to the sample detection device of this application embodiment, and a sleep command can be generated when the conditions of one of the methods are met. For example, the automatic sample introduction mode ends in the first implementation method, which can be applied when automatic sample introduction is performed on a single sample; the automatic sample introduction mode ends in the third implementation method, which can be applied when the automatic sample introduction component is suitable for the sample introduction and detection of multiple samples, so that multiple implementation methods of this embodiment can be applied simultaneously.

[0084] Furthermore, the processor 15 of the sample detection device can generate a sleep command in different sample injection modes of the sample injection module 11, and / or generate a sleep command when the detection of the last sample among multiple samples including the saccharification detection item is completed, to perform the above steps, so that the first detection module 12 switches to a sleep state.

[0085] In one embodiment, the sample detection method further includes: in response to a first operation command for the sample detection device, cleaning the second detection module 13 to switch the second detection module 13 to a preset operation state, and controlling the first detection module 12 to remain in a dormant state; wherein the first operation command is used to instruct the second detection module 13 to switch to the preset operation state, the preset operation state including at least one of a soft-power-on state, a soft-power-off state, a dormant state, and waking up from a dormant state.

[0086] Specifically, the soft-start state of the second detection module 13 can be understood as starting the second detection module 13 by controlling the starting current of the motor, that is, starting the second detection module 13 without disconnecting its power supply. When the first operation command instructs the second detection module 13 to switch to the soft-start state, the second detection module 13 needs to be cleaned before starting to complete the startup process. Similarly, the soft-shutdown state of the second detection module 13 can be understood as shutting down the second detection module 13 by controlling the starting current of the motor, that is, shutting down the second detection module 13 without disconnecting its power supply. When the first operation command instructs the second detection module 13 to switch to the soft-shutdown state, the second detection module 13 needs to be cleaned before shutdown. The sleep state and wake-up from the sleep state of the second detection module 13 are the same as those of the first detection module 12, and will not be described again here.

[0087] In this embodiment, when the second detection module 13 switches from normal detection mode to at least one preset operation state such as soft power-on, soft power-off, sleep mode, or wake-up from sleep mode, the first detection module 12 can remain in sleep mode. That is, the state control between the first detection module 12 and other detection modules of the sample detection device is relatively independent. The first detection module 12 can be controlled to sleep when saccharification detection is not required, and the first detection module 12 can be controlled to wake up from sleep mode when saccharification detection is required, without affecting the operation of other detection modules of the sample detection device, thus improving the practicality of the sample detection device.

[0088] Furthermore, upon receiving a first operation command, the processor 15 of the sample detection device can control the liquid circuit support module 14 to clean the second detection module 13, so that the second detection module 13 switches to a preset operation state, and control the first detection module 12 to remain in a dormant state.

[0089] In one embodiment, the sample detection method further includes cleaning the first detection module 12 and the second detection module 13 in response to a second operation command for the sample detection device, wherein the second operation command is used to instruct the sample detection device to perform a hard boot.

[0090] Specifically, the hard boot of the sample detection device can be understood as the boot mode in which the sample detection device is powered on again after being disconnected from the power supply and the motor is started with the rated current. When the sample detection device performs a hard boot, this embodiment can clean the first detection module 12 and the second detection module 13 so that the second detection module 13 can quickly enter the normal detection mode, while the first detection module 12 can perform the pressure build-up operation after the sample injection module 11 receives the sample including the saccharification detection item. This reduces the consumption of reagents, electricity, etc. by the first detection module 12 in maintaining the detection pressure, thereby reducing the detection cost and improving the utilization rate of saccharification reagents.

[0091] Furthermore, upon receiving a second operation command, the processor 15 of the sample detection device can control the liquid circuit support module 14 to clean the first detection module 12 and the second detection module 13, so that the first detection module 12 and the second detection module 13 can perform subsequent operations.

[0092] Please see Figure 6 , Figure 6 This is a schematic diagram of the structure of an embodiment of the computer-readable storage medium provided in this application. Figure 6 As shown, the computer-readable storage medium of this application stores program instructions capable of implementing all the above methods. These program instructions can be stored in the storage medium in the form of a software product, including several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) or processor to execute all or part of the steps of the methods of each embodiment of this application. The aforementioned storage device includes various media capable of storing program code, such as a USB flash drive, portable hard drive, read-only memory (ROM), random access memory (RAM), magnetic disk, or optical disk, or electronic devices such as computers, servers, mobile phones, and tablets.

[0093] The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A sample detection device, characterized in that, include: The sample introduction module is used to receive the sample to be tested; The first detection module is used to perform saccharification detection on the sample to be tested; The second detection module is used to perform detection on the sample to be tested, excluding the saccharification detection item; A liquid circuit support module is connected to the first detection module; The processor is connected to the sample injection module, the first detection module, and the second detection module respectively. The processor is used to control the liquid path support module to clean the first detection module when controlling the second detection module to detect the sample, so that the first detection module switches to a sleep state or wakes up from the sleep state.

2. A sample detection method, characterized in that, Applied to the sample detection apparatus as described in claim 1, the sample detection method includes: Receive the sample to be tested; When the second detection module of the sample detection device performs tests on the sample other than saccharification detection, the first detection module of the sample detection device is cleaned so that the first detection module switches to the dormant state or wakes up from the dormant state.

3. The sample detection method according to claim 2, characterized in that, When the second detection module of the sample detection device performs tests on the sample other than saccharification detection, the first detection module of the sample detection device is cleaned, including: When the sample injection module of the sample detection device is in automatic injection mode, the second detection module detects the sample, and the first detection module is in the dormant state, if the first sample including the saccharification detection item is received, the first detection module is cleaned and pressure built up, and the first detection module is controlled to perform saccharification detection on the sample including the saccharification detection item.

4. The sample detection method according to claim 2, characterized in that, The step of cleaning the first detection module of the sample detection device to switch the first detection module to a dormant state or wake it up from the dormant state includes: Obtain the testing items for multiple samples to be tested; When the saccharification detection item is included in the detection items of the multiple samples, the time interval between the first sample that includes the saccharification detection item and the current detection position is obtained; When the interval is less than or equal to a first preset time, a cleaning operation or a cleaning pressure build-up operation is performed on the first detection module to wake it up from the dormant state.

5. The sample detection method according to claim 2, characterized in that, The step of cleaning the first detection module of the sample detection device to switch the first detection module to a dormant state or wake it up from the dormant state includes: In response to the triggering of the sleep / wake button of the first detection module, a cleaning operation is performed on the first detection module to wake it from the sleep state. When the sample injection module receives a sample including the saccharification detection item, it performs a pressure build-up operation on the first detection module and controls the first detection module to perform saccharification detection on the sample including the saccharification detection item.

6. The sample detection method according to claim 2, characterized in that, The sample detection method includes: When the first detection module is in a pressure maintenance state, upon receiving a sleep command from the first detection module, the first detection module is controlled to continue maintaining the pressure maintenance state for a second preset time. If a sample including the saccharification detection item is received within the second preset time, the first detection module is controlled to detect the sample including the saccharification detection item. If no sample including the saccharification detection item is received within the second preset time, the first detection module is cleaned so that the first detection module switches to the dormant state.

7. The sample detection method according to claim 6, characterized in that, The control of the first detection module to continue maintaining the pressure maintenance state for the second preset time includes: The control fluid support module continuously supplies eluent to the detection pipeline of the first detection module so that the first detection module is maintained in the pressure maintenance state; The second preset time range is the time range within which the first volume of the eluent consumed by the detection pipeline is less than or equal to the second volume of the eluent used by the first detection module to complete one saccharification detection item.

8. The sample detection method according to claim 6, characterized in that, The sample detection method includes: The sleep command is generated when the automatic sample introduction mode of the sample introduction module of the sample detection device ends; or, The dormant command is generated after the current sample detection device completes the detection of the sample using either closed or open injection mode; or... When the sample injection end button of the sample injection module of the sample detection device is triggered, the sleep command is generated; or, Based on the detection items of multiple samples to be tested, if the detection items of the multiple samples include the saccharification detection item, the dormancy command is generated when the last sample including the saccharification detection item is tested.

9. The sample detection method according to claim 2, characterized in that, The sample detection method further includes: In response to a first operation command for the sample detection device, the second detection module is cleaned to switch the second detection module to a preset operation state, and the first detection module is controlled to remain in the dormant state. The first operation instruction is used to instruct the second detection module to switch to a preset operation state, which includes at least one of the following: soft power-on state, soft power-off state, hibernation state, and waking up from hibernation state.

10. The sample detection method according to claim 2, characterized in that, The sample detection method further includes: In response to a second operation command for the sample detection device, the first detection module and the second detection module are cleaned, wherein the second operation command is used to instruct the sample detection device to perform a hard boot.

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