A composite biological pond cleaning agent containing tea saponin and a preparation method thereof

Abstract

This invention belongs to the field of biological agents for aquaculture, and discloses a compound biological pond-cleaning agent containing tea saponin and its preparation method. The compound biological pond-cleaning agent of this invention comprises the following components by weight: 3-10 parts tea saponin, 60-70 parts roe beetle root powder, 10-20 parts Buddleja officinalis, 5-10 parts compound microbial powder, 3-8 parts adjuvants, and 10-20 parts carrier; the adjuvant is a mixture of sodium lignin sulfonate and sodium dodecylbenzene sulfonate; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria; and the carrier is diatomaceous earth. This invention combines three natural active ingredients—tea saponin, roe beetle root powder, and Buddleja officinalis—with suitable adjuvants and a carrier, which can expand the pond-cleaning range and has good killing or inhibitory effects on harmful organisms such as wild fish, shrimp, crabs, and snails, as well as various pathogens. The pond-cleaning is thorough, and the raw materials are easily degradable, leaving no residue. It is environmentally friendly and has a long-lasting effect, which is of great significance for promoting the green and sustainable development of aquaculture.

Description

Technical Field

[0001] This invention relates to the field of biological agents for aquaculture, specifically to a compound biological pond cleaning agent containing tea saponin and its preparation method. Background Technology

[0002] In aquaculture, pond cleaning is a crucial preparatory step. Its purpose is to effectively kill harmful organisms such as wild fish, snails, pathogens, and parasites in the pond, creating a safe and stable growth environment for the farmed organisms. Traditional pond cleaning methods often use chemical reagents such as lime and bleaching powder. These chemical pond cleaning agents have drawbacks such as non-selective toxicity, long residual periods, and the potential to disrupt the aquatic ecological balance. This not only poses a potential threat to subsequently farmed fish and shrimp but also pollutes the surrounding aquatic environment, which is inconsistent with the development concept of green and ecological aquaculture.

[0003] To address the drawbacks of chemical pond cleaning agents, plant-derived biological pond cleaning agents have gradually become a research hotspot. Among them, tea saponin, a triterpenoid saponin extracted from camellia oil seed cake, has significant hemolytic toxicity to fish. It can enter the bloodstream by destroying the gill epithelial cells of fish, leading to the disintegration of red blood cells. However, it is non-toxic to crustaceans such as shrimp and crabs, and can even promote their molting and growth. Moreover, it can be decomposed naturally or by microorganisms, making it highly environmentally friendly.

[0004] Existing technologies include compound pond-cleaning agents containing tea saponins and rotenone, but there is still room for improvement in the range of pathogen inhibition and the broad-spectrum killing of harmful organisms. Furthermore, the use of single plant-derived ingredients often results in large dosages, slow onset of action, or insufficient targeting. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention develops a composite biological pond cleaning agent containing tea saponin and its preparation method. The agent combines three natural active ingredients—tea saponin, rotenone root powder, and Buddleja officinalis—with suitable adjuvants and carriers. This expands the pond cleaning range and exhibits excellent killing or inhibitory effects on harmful organisms such as wild fish, shrimp, crabs, and snails, as well as various pathogens. The pond cleaning is thorough, and the raw materials are easily degradable, leaving no residue. It is environmentally friendly and has a long-lasting effect.

[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: A compound biological pond-cleaning agent containing tea saponin, by weight, comprises the following components: 3-10 parts tea saponin, 60-70 parts roe beetle root powder, 10-20 parts Buddleja officinalis, 5-10 parts compound microbial powder, 3-8 parts adjuvants, and 10-20 parts carrier; the adjuvants are a mixture of sodium lignin sulfonate and sodium dodecylbenzene sulfonate; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria; and the carrier is diatomaceous earth. Roe beetle root powder inhibits the mitochondrial respiratory chain, interfering with the energy metabolism of harmful organisms, thus achieving contact and stomach poison effects. It is highly effective in killing wild fish and planktonic pests, and is easily decomposed in light, leaving very little residue, making it relatively safe for shrimp and crabs. Buddleja officinalis has natural effects in pond cleaning, disinfection, insecticidal, and antibacterial properties, and contains active ingredients such as buddleja glycoside and verbascoside, possessing certain antioxidant activity. The Bacillus subtilis and photosynthetic bacteria in the compound microbial powder can effectively inhibit the reproduction of pathogenic microorganisms such as Vibrio and Escherichia coli in the water, while decomposing organic pollutants in the bottom sediment and improving the bottom environment. The adjuvants can significantly improve the dispersibility and solubility of each active ingredient in the water, enhancing the uniformity of the pond-cleaning effect. The carrier can slow down the release rate of the active ingredients, prolong the duration of the pond-cleaning agent's effect, reduce the frequency of pond cleaning, and lower aquaculture costs.

[0007] Preferably, the weight ratio of sodium lignosulfonate to sodium dodecylbenzenesulfonate is 2:1 to 3:1.

[0008] Preferably, the weight ratio of Bacillus subtilis to photosynthetic bacteria in the compound microbial powder is 2:1.

[0009] Preferably, the compound biological pond-cleaning agent comprises, by weight, the following components: 8 parts tea saponin, 65 parts rotenone root powder, 15 parts Buddleja officinalis, 7 parts compound microbial powder, 5 parts adjuvant, and 15 parts carrier. This preferred formula, through the synergistic effect of its components, can further enhance the ecological restoration of the aquatic body and extend its effective period while ensuring efficient pond cleaning.

[0010] This invention also provides a method for preparing a compound biological pond-cleaning agent containing tea saponin, comprising the following steps: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 20-30 minutes at a speed of 150-200 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 10-15 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 3-5 portions, stirring for 5-8 minutes after each addition, and finally stirring at high speed for 20-30 minutes at a speed of 300-400 r / min; (5) After passing the sampling inspection, the samples are packaged in a fixed quantity.

[0011] When using the compound biological pond cleaning agent of this invention to clean ponds, use 1.0-1.5 kg per 667 cubic meters of water, soak in 30-40℃ warm water for 30 minutes, and then spray it throughout the pond.

[0012] Compared with existing technologies, the beneficial effects of adopting the above technical solution are as follows: This invention combines three natural active ingredients—tea saponin, rotenone root powder, and Buddleja officinalis—with suitable adjuvants and carriers to expand the pond clearing range. It has a good killing or inhibitory effect on harmful organisms such as wild fish, shrimp, crabs, and snails, as well as various pathogens. The pond clearing is thorough, and the raw materials are easily degradable, leaving no residue. It is ecological, environmentally friendly, and has a long-lasting effect, which is of great significance for promoting the green and sustainable development of aquaculture.

[0013] The preparation method of the present invention does not require complex equipment and processes, is simple to operate, and is easy to scale up for production. Detailed Implementation

[0014] The present invention will be further described in detail below with reference to specific embodiments. Example

[0015] A compound biological pond cleaning agent containing tea saponin, comprising the following components by weight: 8 parts tea saponin, 65 parts rotenone root powder, 15 parts Buddleja officinalis, 7 parts compound microbial powder, 5 parts adjuvant, and 15 parts carrier.

[0016] The additives are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate, with a weight ratio of 2:1; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria, with a weight ratio of 2:1; and the carrier is diatomaceous earth.

[0017] Preparation method: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 30 minutes at a speed of 200 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 15 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 5 portions, stirring for 5 minutes after each addition, and finally stir at high speed for 30 minutes at a speed of 400 r / min; (5) After passing the sampling inspection, the samples are packaged in a fixed quantity. Example

[0018] A compound biological pond cleaning agent containing tea saponin, by weight, comprises the following components: 3 parts tea saponin, 70 parts rotenone root powder, 18 parts Buddleja officinalis, 6 parts compound microbial powder, 3 parts adjuvant, and 20 parts carrier. The additives are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate, with a weight ratio of 3:1; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria, with a weight ratio of 2:1; and the carrier is diatomaceous earth.

[0019] Preparation method: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 30 minutes at a speed of 150 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 10 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 4 portions, stirring for 8 minutes after each addition, and finally stirring at high speed for 30 minutes at a speed of 400 r / min; (5) After passing the sampling inspection, the samples are packaged in a fixed quantity. Example

[0020] A compound biological pond cleaning agent containing tea saponin, comprising the following components by weight: 10 parts tea saponin, 60 parts rotenone root powder, 20 parts Buddleja officinalis, 5 parts compound microbial powder, 8 parts adjuvant, and 15 parts carrier. The additives are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate, with a weight ratio of 3:1; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria, with a weight ratio of 2:1; and the carrier is diatomaceous earth.

[0021] Preparation method: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 25 minutes at a speed of 180 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 13 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 4 portions, stirring for 6 minutes after each addition, and finally stirring at high speed for 25 minutes at a speed of 350 r / min; (5) After passing the sampling inspection, the samples are packaged in fixed quantities. Example

[0022] A compound biological pond cleaning agent containing tea saponin, comprising the following components by weight: 5 parts tea saponin, 70 parts rotenone root powder, 15 parts Buddleja officinalis, 8 parts compound microbial powder, 6 parts adjuvant, and 10 parts carrier. The additives are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate, with a weight ratio of 3:1; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria, with a weight ratio of 2:1; and the carrier is diatomaceous earth.

[0023] Preparation method: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 20 minutes at a speed of 200 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 10 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 5 portions, stirring for 5 minutes after each addition, and finally stir at high speed for 20 minutes at 300 r / min; (5) After passing the sampling inspection, the samples are packaged in fixed quantities. Example

[0024] A compound biological pond cleaning agent containing tea saponin, comprising the following components by weight: 6 parts tea saponin, 65 parts rotenone root powder, 10 parts Buddleja officinalis, 10 parts compound microbial powder, 5 parts adjuvant, and 16 parts carrier. The additives are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate, with a weight ratio of 2.5:1; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria, with a weight ratio of 2:1; and the carrier is diatomaceous earth.

[0025] Preparation method: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 30 minutes at a speed of 150 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 15 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 3 portions, stirring for 8 minutes after each addition, and finally stirring at high speed for 30 minutes at a speed of 400 r / min; (5) After passing the sampling inspection, the samples are packaged in fixed quantities. When using the compound biological pond cleaning agent of this invention to clean ponds, use 1.0-1.5 kg per 667 cubic meters of water, soak in 30-40℃ warm water for 30 minutes, and then spray it throughout the pond.

[0026] Experimental Example: Effect Verification Experiment of Examples 1-3: Four experimental ponds, each 1 mu in area and 1 m in depth, were selected and labeled as experimental group 1, experimental group 2, experimental group 3, and control group. Experimental groups 1-3 were treated with the compound biological pond-cleaning agent prepared in Examples 1-3 of this invention at a rate of 1.5 kg / mu; the control group was treated with the conventional chemical pond-cleaning agent quicklime at a rate of 100 kg / mu. The mortality rate of wild fish, ammonia nitrogen content, nitrite content, and beneficial bacteria count in each pond were measured at 7, 15, and 30 days after pond cleaning, and water transparency was also observed.

[0027] The experimental results showed that 7 days after pond clearing, the mortality rate of wild fish in experimental groups 1-3 reached over 98%, while the mortality rate in the control group was 95%. 15 days after pond clearing, the ammonia nitrogen content in experimental groups 1-3 was ≤0.1mg / L, the nitrite content was ≤0.05mg / L, the number of beneficial bacteria was ≥10 CFU / mL, and the water transparency was ≥40cm. In contrast, the ammonia nitrogen content in the control group was 0.3mg / L, the nitrite content was 0.12mg / L, the number of beneficial bacteria was ≤10 CFU / mL, and the water transparency was 30cm. 30 days after pond clearing, the various water indicators in experimental groups 1-3 remained stable, and the number of beneficial bacteria continued to increase, while the ammonia nitrogen and nitrite content in the control group increased, and the number of beneficial bacteria increased slowly.

[0028] The above test results show that the composite biological pond cleaning agent of the present invention has a better pond cleaning effect than conventional chemical pond cleaning agents, and can effectively improve the aquatic environment, restore the ecological balance of the aquatic body, and has a long-lasting effect.

[0029] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A compound biological pond-cleaning agent containing tea saponin, characterized in that: The product comprises, by weight, the following components: 3-10 parts tea saponin, 60-70 parts roe vine root powder, 10-20 parts Buddleja officinalis, 5-10 parts compound microbial powder, 3-8 parts adjuvants, and 10-20 parts carrier; the adjuvants are a mixture of sodium lignosulfonate and sodium dodecylbenzenesulfonate; the compound microbial powder is a mixture of Bacillus subtilis and photosynthetic bacteria; and the carrier is diatomaceous earth.

2. The composite biological pond-cleaning agent containing tea saponin according to claim 1, characterized in that: The weight ratio of sodium lignosulfonate to sodium dodecylbenzenesulfonate is 2:1 to 3:

1.

3. The composite biological pond-cleaning agent containing tea saponin according to claim 1, characterized in that: The weight ratio of Bacillus subtilis to photosynthetic bacteria in the compound microbial powder is 2:

1.

4. The composite biological pond-cleaning agent containing tea saponin according to claim 1, characterized in that: By weight, it includes the following components: 8 parts tea saponin, 65 parts roe vine root powder, 15 parts Buddleja officinalis, 7 parts compound microbial powder, 5 parts adjuvant, and 15 parts carrier.

5. The preparation method of a composite biological pond-cleaning agent containing tea saponin according to claim 1, characterized in that, Includes the following steps: (1) The tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder were pulverized and passed through a 120-mesh sieve, and the diatomaceous earth was dried to a moisture content of ≤5%; (2) Weigh out tea saponin, roe vine root powder, Buddleja officinalis and compound microbial powder according to the proportion, add them to the mixer and stir at low speed for 20-30 minutes at a speed of 150-200 r / min; (3) Add sodium lignosulfonate and sodium dodecylbenzenesulfonate, and continue stirring for 10-15 minutes at a speed of 200 r / min; (4) Add diatomaceous earth in 3-5 portions, stirring for 5-8 minutes after each addition, and finally stirring at high speed for 20-30 minutes at a speed of 300-400 r / min; (5) After passing the sampling inspection, the samples are packaged in a fixed quantity.