Preparation method of bio-enzyme decomposed ore oxygen-providing soil remediation agent

Abstract

The application provides a preparation method of a biological enzymatic ore oxygen-providing soil remediation agent, and comprises the following steps: S1, animal raw material enzymolysis, complete decomposition into small molecule amino acids, and then high-temperature sterilization; S2, skinning: skinned into crude protein through a skimming furnace; S3, mixed fermentation: kitchen waste and minerals are mixed together in a certain proportion, and then fermentation is carried out; S4, auxiliary material adjustment fermentation: adding leftover materials of a monosodium glutamate factory, sugar juice and bacteria, and adding mineral potassium fulvate to adjust pH value and carbon neutralization, and then fermentation is carried out; S5, grinding and screening: grinding and screening are carried out through a ball mill; and S6, one-time boiling: the screened material is subjected to biological enzymatic fermentation boiling. The application can significantly improve the stress resistance of crops, increase yield, increase organic matter and oxygen content of land, effectively remove hardening and salinization, and reduce the use of pesticides and industrial chemical fertilizers, and solves the problems of high cost, short effect and easy environmental pollution caused by traditional soil improvement methods.

Description

Technical Field

[0001] This invention relates to the field of soil remediation agent technology, and in particular to a method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent. Background Technology

[0002] Compacted soil refers to soil with a hardened, dense, and impermeable plate-like structure formed on the surface due to excessively fine soil particles, low organic matter content, and damaged soil structure. Compacted soil severely affects soil permeability, water retention, nutrient retention, and biological activity, reducing soil fertility and yield, and is one of the important factors restricting sustainable agricultural development.

[0003] Currently, commonly used methods for remediating compacted soil mainly include physical remediation, chemical remediation, and biological remediation. Physical remediation involves deep plowing, loosening, and mulching to break up the compacted layer, increasing soil porosity and permeability. Chemical remediation involves applying alkaline substances such as lime and gypsum to neutralize soil acidity, increase soil pH, and improve soil chemical properties. Biological remediation involves applying organic fertilizers, microbial agents, and plant root systems to increase soil organic matter content, promote soil microbial activity, and improve soil biological properties.

[0004] However, these methods all have certain limitations and drawbacks. For example, physical remediation methods require a lot of manpower, material resources, and financial resources, and the effects are not lasting; chemical remediation methods may cause soil salinization or heavy metal pollution, and have certain harms to the environment; biological remediation methods take a long time to show results and are affected by climate and seasonal factors.

[0005] Therefore, there is an urgent need for a new type of compacted soil remediation agent, as well as its preparation and application methods, that can quickly and effectively improve the physical and chemical properties and biological activity of compacted soil while reducing its adverse environmental impact. Summary of the Invention

[0006] In view of this, the present invention aims to provide a method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent, so as to solve or alleviate the technical problems existing in the prior art.

[0007] To solve the above-mentioned technical problems, the technical solution adopted in this application is: a method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent, comprising the following steps: S1 Animal Raw Material Enzymatic Hydrolysis: Slaughterhouse by-products and dead poultry, pigs, cattle, etc. from pollution-free treatment plants are first crushed, and then bio-enzymatically hydrolyzed in a reaction vessel at a low temperature of about 50 degrees Celsius. Alkaline enzymes, neutral enzymes, and acidic enzymes are used. On the basis of enzymatic hydrolysis, potassium humate from mineral source is added to decompose it completely into small molecule amino acids, and then it is sterilized at high temperature. S2 Animal hide processing: Animal hides are processed into crude protein by passing them through a chemical furnace at a high temperature of 138 degrees Celsius. S3 Mixed Fermentation: Vegetable oil residue (peanut, sunflower seed, cotton, soybean, etc.), bones, eggshells, and well-processed crude protein, kitchen waste (bitter bean, walnut husk) and minerals are mixed together in a certain proportion, crushed, and then fermented; wherein, the minerals include stone, pearl stone, jade, agate stone fragments, and zeolite. S4 Auxiliary Material Fermentation Adjustment: Add some auxiliary materials, including MSG factory scraps, syrup and bacteria, and add mineral-derived potassium humate to adjust the pH value and carbon neutralize, and ferment together, while also increasing minerals and macro- and micro-elements; S5 Grinding and Sieving: Grind together with all fermented materials without adding water. Use enzymatically hydrolyzed amino acids and meat broth, grind them into 150-300 mesh or higher using a ball mill, and then sieve them. S6 One-time Boiling: After sieving, it undergoes biological enzymatic hydrolysis and fermentation boiling; S7 Secondary Boiling and Aging: Acidic enzymes and citric acid are added, along with mineral powder and a small amount of leftover materials from MSG and food factories. The mixture undergoes secondary enzymatic fermentation and aging.

[0008] A bio-enzymatic hydrolysis ore oxygen-supplying soil remediation agent, wherein the soil remediation agent is a mixture composed of enzymatically hydrolyzed amino acids, chemically processed crude protein, fermented oil residue, bone meal, eggshell mixture, and ore powder; wherein several of the ores can provide oxygen to the materials, enabling the materials to undergo aerobic fermentation.

[0009] This invention also provides the application of the bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent in crop cultivation, wherein the crops include various crops, fruits and vegetables, cotton, corn, soybeans, etc.; the fertilization methods include root application, foliar application, and drip irrigation.

[0010] The embodiments of this invention, due to the adoption of the above technical solutions, have the following advantages: By using a bio-enzymatic hydrolysis process combined with high-temperature processing, slaughterhouse by-products, dead poultry and livestock, and hides are converted into small-molecule amino acids and crude protein, and the decomposition is promoted more thoroughly by utilizing mineral-derived potassium humate and enzymatic hydrolysis; by mixing oil residue, bones, eggshells, and specific minerals (stone, pearl stone, jade, agate fragments, zeolite) in a certain proportion and supplementing them with monosodium glutamate factory by-products, syrup, and bacteria for fermentation, aerobic fermentation is achieved by utilizing the oxygen provided by the ore, which effectively improves the maturity of the materials; by using a ball mill to grind the materials to 150-300 mesh or higher without adding water, and by using enzymatically hydrolyzed amino acids and meat broth, the materials undergo secondary bio-enzymatic hydrolysis, fermentation, simmering, and aging, resulting in products with higher activity and more comprehensive nutrition. The resulting soil remediation agent can significantly improve crop stress resistance, increase yield, increase organic matter and soil oxygen content, effectively remove soil compaction and salinization, and reduce the use of pesticides and industrial fertilizers, solving the problems of high cost, short-lasting effect and easy environmental pollution caused by traditional soil improvement methods.

[0011] The above overview is for illustrative purposes only and is not intended to be limiting in any way. Further aspects, embodiments, and features of the invention will become apparent from the following detailed description, in addition to the illustrative aspects, embodiments, and features described above. Detailed Implementation

[0012] The following is a detailed description of the embodiments of this disclosure.

[0013] It should be understood that the following specific examples illustrate the implementation of this disclosure, and those skilled in the art can easily understand other advantages and effects of this disclosure from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of this disclosure, and not all of them. This disclosure can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this disclosure. It should be noted that, in the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0014] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for preparing a bio-enzymatic hydrolysis ore oxygen-supplying soil remediation agent. This method realizes the efficient resource utilization of agricultural and industrial waste, and achieves aerobic composting in the fermentation process through the oxygen supply of specific ores, thereby improving product quality. At the same time, it provides the soil remediation agent prepared by this method and its application. The resulting remediation agent can effectively improve soil compaction and salinization problems, increase soil organic matter and oxygen content, and improve crop stress resistance and yield.

[0015] A method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent includes the following steps: S1 Animal Raw Material Enzymatic Hydrolysis: After removing impurities from slaughterhouse by-products, diseased and dead poultry, diseased and dead pigs and cattle from pollution-free treatment plants, animal raw materials are crushed to a particle size of 5-10mm and put into a reaction vessel for biological enzymatic hydrolysis under low temperature conditions of 50℃±2℃.

[0016] During the enzymatic hydrolysis process, alkaline enzyme, neutral enzyme, and acidic enzyme (in a mass ratio of 2:3:2) are added, with a total addition amount of 0.5-1.0 wt% of the animal raw material mass. At the same time, 2-3 wt% of mineral-derived potassium humate is added, and the mixture is continuously stirred (60-80 r / min) to promote the complete decomposition of the raw material into small molecule amino acids (molecular weight ≤1000 Da). The enzymatic hydrolysis time is 8-12 h. After enzymatic hydrolysis, the temperature of the reactor is raised to 121°C and kept at that temperature for 30 minutes for high-temperature sterilization to remove harmful microorganisms and parasite eggs.

[0017] The low temperature environment of 50℃±2℃ can prevent the loss of enzyme activity and ensure that alkaline enzymes, neutral enzymes and acidic enzymes work together to meet the decomposition needs of different components such as proteins and fats in animal raw materials. Potassium humate from mineral sources, as a natural chelating agent and decomposition promoter, can reduce the activation energy of enzymatic hydrolysis, promote the conversion of macromolecules into small amino acids, and improve the bioavailability of the product; the 121℃ high-temperature sterilization adopts the principle of moist heat sterilization, which can thoroughly kill pathogenic bacteria such as Escherichia coli and Salmonella, as well as parasite eggs that may be present in the raw materials, ensuring the safety of product application.

[0018] S2 Fur Processing: After removing impurities (mud, sand, hair fragments) from animal fur, it is sent to a processing furnace and processed at a high temperature of 138℃±5℃ and a pressure of 0.15MPa for 2-3 hours to obtain crude protein (crude protein content ≥80%).

[0019] The high temperature and high pressure environment of 138℃±5℃ can destroy the spatial structure of keratin in animal fur, degrading it into crude protein that can be utilized by microorganisms, while achieving sterilization and disinfection (sterilization rate ≥99.9%). This transforms solid waste that is difficult to use directly into high-quality fermentation raw materials, realizing resource recycling.

[0020] S3 Mixed Fermentation: Vegetable oil residue (peanut, sunflower seed, cotton, soybean, etc., with moisture content ≤10%), bones (crushed to 20 mesh), eggshells (crushed to 30 mesh) are mixed with crude protein obtained in step S2 at a mass ratio of 3:2:1:2. Kitchen waste (bitter bean, walnut green skin) and minerals are mixed at a mass ratio of 10:(3-5). The mixture is then crushed to 80 mesh and sent to a fermentation tank for fermentation. Fermentation temperature should be controlled at 30-35℃, fermentation time is 15-20 days, and the mixture should be turned twice a day (30 minutes each time).

[0021] The minerals include zeolite, pearlite, jade, agate fragments, and zeolite, mixed in a mass ratio of 2:2:1:1:4, with a particle size of 50-100 mesh.

[0022] Vegetable oil residue is rich in fat and carbon sources, bones are rich in phosphorus, calcium, and collagen, and eggshells are rich in calcium carbonate. Together with crude protein, these materials can provide abundant carbon, nitrogen, and mineral nutrients for fermenting microorganisms. Stone, pearl stone, jade, agate fragments, and zeolite all have porous structures with a porosity of 40-60%. During fermentation, they can adsorb and slowly release oxygen, providing a continuous oxygen supply for the aerobic metabolism of microorganisms and solving the problem of insufficient oxygen supply in traditional fermentation. Crushing materials to 80 mesh can increase the specific surface area, promote full contact between microorganisms and materials, and improve fermentation efficiency. 30-35℃ is the optimal growth temperature for fermenting microorganisms (Bacillus, yeast, etc.). Daily turning and tossing can further supplement oxygen and avoid the production of foul-smelling gases from localized anaerobic fermentation.

[0023] S4 Additive Fermentation Adjustment: Based on the fermentation system in step S3, add auxiliary materials (MSG factory scraps, syrup, and bacteria mixed in a mass ratio of 4:3:1), with the amount of auxiliary materials added being 5-8 wt% of the mass of the mixture in step S3; at the same time, add 1-2 wt% of mineral-derived potassium humate to adjust the pH of the fermentation system to 6.5-7.5 to achieve carbon neutralization; Continue co-fermentation at 30-35℃ for 5-8 days. During fermentation, supplement minerals (same as step S3 mineral formula, the amount added is 2-3wt% of the mass of the mixture) and macro-, meso-, and micro-elements (total nitrogen, phosphorus, and potassium content ≥5%, total calcium, magnesium, and sulfur content ≥3%, total iron, zinc, copper, boron, and molybdenum content ≥0.5%).

[0024] The by-products from monosodium glutamate (MSG) factories are rich in monosodium glutamate and amino acids, while syrup provides an easily available carbon source. Both promote the proliferation of fermenting microorganisms and enhance fermentation intensity. The selected microorganisms are a compound inoculum of Bacillus subtilis and yeast (effective viable count ≥10). 8 (CFU / g) can directionally decompose macromolecules in materials and accelerate material decomposition; the pH regulation effect of mineral-derived potassium humate can maintain the acid-base balance of the fermentation system, creating a suitable environment for microbial growth; its carbon neutralization properties can adsorb harmful gases such as ammonia and hydrogen sulfide produced during fermentation, reducing environmental pollution; the supplemented minerals and macro- and micro-elements can enrich the nutritional composition of the product and enhance the comprehensive soil improvement efficacy of the remediation agent.

[0025] S5 Grinding and Sieving: All materials fermented in step S4 (moisture content ≤15%) are ground together without adding extra water. The amino acids and meat broth obtained from enzymatic hydrolysis in step S1 (mass ratio 1:1) are used as the grinding medium, and the amount of medium added is 10-15 wt% of the fermented material mass. Grind the material in a ball mill (300-400 r / min) for 2-3 hours to achieve a fineness of 150-300 mesh. Then, use a double-layer vibrating screen (150 mesh in the upper layer and 300 mesh in the lower layer) for sieving. Collect the material under the lower screen as qualified material, and return the material over the screen to the ball mill for re-grinding.

[0026] Using amino acids and meat broth as grinding media can avoid material clumping and loss of effective ingredients caused by adding water. At the same time, amino acids can act as dispersants to improve grinding efficiency. The high-frequency impact and grinding action of the ball mill can refine the material particles. The fineness of 150-300 mesh can ensure that the remediation agent is evenly dispersed in the soil, increase the contact area with soil particles, and improve the effect. The classification and screening of the double-layer vibrating screen can ensure that the product particle size is uniform and avoid coarse particles from affecting the application effect.

[0027] S6 Single-Stage Boiling: The sieved, qualified material is fed into a boiling tank, and 0.3-0.5 wt% of a compound enzyme (alkaline enzyme: neutral enzyme = 1:1) is added. Biological enzymatic fermentation is carried out at 45-50℃ and a rotation speed of 50 r / min for 7-10 days. During this period, oxygen is introduced once daily (1 hour each time, oxygen concentration ≥21%). The 45-50℃ temperature maintains the activity of the compound enzyme, further degrading residual macromolecules in the material and increasing the content of active ingredients in the product. Oxygenation ensures an aerobic environment for enzymatic fermentation, preventing anaerobic metabolism that could lead to a decline in product quality.

[0028] S7 Secondary Boiling and Aging: Add 0.2-0.3 wt% acidic enzyme, 0.5-1.0 wt% citric acid, 5-10 wt% mineral powder (same as the mineral formula in step S3, pulverized to 300 mesh) and 3-5 wt% MSG factory and food factory scraps (mass ratio 1:1, pulverized to 80 mesh) to the material after the first boiling. Carry out secondary biological enzymatic hydrolysis fermentation boiling at 40-45℃ and 40 r / min for 10-15 days. After boiling and mixing, the materials are sent to an aging chamber and aged for 15-20 days at 25-30℃ and 60-70% relative humidity to obtain the finished product of bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent. The acidic enzymes and citric acid work synergistically to adjust the system pH to 5.5-6.0, adapting to the optimal reaction environment of the acidic enzymes and further degrading residual macromolecular substances; 300-mesh ore powder supplements the oxygen supply function while increasing the mineral content of the product. The byproducts from MSG and food factories provide additional nutrition and enhance the product's fertility; the aging process stabilizes the product's performance, fully converts the active ingredients, and improves the longevity of its application effects.

[0029] Bio-enzymatic hydrolysis of mineral oxygen-supplying soil remediation agent The soil remediation agent is a mixture processed through steps S1-S7 above. It is a brownish-brown powder with a moisture content ≤12%, pH 6.5-7.5, organic matter content ≥45%, small molecule amino acid content ≥8%, and effective viable bacteria count ≥10. 6 CFU / g, particle size ≥150 mesh.

[0030] Among them, minerals such as nephrite, pearlite, jade, agate fragments, and zeolite, with their porous structure, provide continuous oxygen during the fermentation stages (S3, S4) of the preparation process, ensuring smooth aerobic fermentation and achieving a material maturity of ≥90%. The residual mineral particles in the finished product can continue to play a role in aeration after being applied to the soil, increasing soil oxygen content. This soil remediation agent can improve crop stress resistance (drought and disease resistance increased by 20-30%), increase crop yield (yield increase of 10-15%), increase soil organic matter content (applying 200 kg per acre can increase soil organic matter content by 0.2-0.3 percentage points), and increase soil oxygen content (to 12-15%), effectively improving soil compaction (soil bulk density reduced to 1.2-1.3 g / cm³). 3 This addresses issues such as salinization (EC value reduced by 30-40%) and reduces the use of pesticides and industrial fertilizers (reduced by 20-30%).

[0031] Application of bio-enzymatic hydrolysis of mineral oxygen-supplying soil remediation agents The soil remediation agent is applied to crop cultivation, and is suitable for various grain crops (wheat, rice, corn, etc.), fruits and vegetables (tomatoes, cucumbers, apples, citrus, etc.), and cash crops (cotton, soybeans, peanuts, etc.). Fertilization methods include root application (200-300 kg per mu, applied in conjunction with tillage), foliar application (diluted 500-800 times, sprayed on leaves, 2-3 times during the growing season), and drip irrigation (diluted 1000-1500 times, applied with the drip irrigation system, 50-100 kg per mu each time, 3-4 times during the growing season).

[0032] Example 1 A method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent includes the following steps: S1. Enzymatic hydrolysis of animal raw materials: Slaughterhouse waste and diseased or dead pigs and cattle are crushed to 5mm and put into a reaction vessel for biological enzymatic hydrolysis at 48℃. Alkaline enzyme, neutral enzyme and acidic enzyme (mass ratio 2:3:2) are added, with a total addition amount of 0.5wt% of the animal raw material mass. At the same time, 2wt% of the animal raw material mass of mineral-derived potassium humate is added. The rotation speed is 60r / min, and the enzymatic hydrolysis is carried out for 8 hours. After the enzymatic hydrolysis is completed, it is sterilized by keeping it at 121℃ for 30 minutes.

[0033] S2. Fur rendering: After removing impurities from the animal fur, it is sent to a rendering furnace and rendered at 133℃ and 0.15MPa for 2 hours to obtain crude protein (crude protein content 80%).

[0034] S3. Mixed Fermentation: Mix soybean residue, bones (20 mesh), eggshells (30 mesh) with S2 crude protein in a mass ratio of 3:2:1:2, then mix with minerals (stone: pearl stone: jade stone: agate stone fragments: zeolite = 2:2:1:1:4, 50 mesh) in a mass ratio of 10:3, grind to 80 mesh, send to fermentation tank, ferment at 30℃ for 15 days, turning twice a day.

[0035] S4. Additive adjustment for fermentation: Add auxiliary ingredients (MSG factory scraps: syrup: bacteria = 4:3:1), the amount added is 5wt% of the mass of the mixture in S3, and at the same time add 1wt% of mineral potassium humate, adjust the pH to 6.5, continue fermentation at 30℃ for 5 days, and supplement 2wt% of minerals and 3wt% of macro and micro elements.

[0036] S5. Grinding and sieving: Mix the fermented material with the amino acids and meat juice (1:1) from S1 at a mass ratio of 10:1. Grind the material in a ball mill at 300 r / min for 2 hours to 150 mesh. Then, sieve the material through a double-layer vibrating screen and return the material on the screen to the grinding mill.

[0037] S6. First cooking: Send the sieved material into the cooking tank, add 0.3wt% of compound enzyme (alkaline enzyme: neutral enzyme = 1:1), cook at 45℃ and 50r / min for 7 days, and purify with oxygen for 1 hour each day.

[0038] S7. Secondary boiling and aging: Add 0.2wt% acidic enzyme, 0.5wt% citric acid, 5wt% 300-mesh ore powder and 3wt% MSG factory and food factory scraps (1:1), boil at 40℃ and 40r / min for 10 days; age at 25℃ and 60% relative humidity for 15 days to obtain the finished product.

[0039] Finished product specifications: Brownish-brown powder, 10% moisture, pH 6.5, 45% organic matter content, 8% small molecule amino acid content, and 1.2 × 10⁻⁶ viable bacteria count. 6 CFU / g, fineness 150 mesh.

[0040] Example 2 A method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent includes the following steps: S1. Enzymatic hydrolysis of animal raw materials: Slaughterhouse waste and dead poultry are crushed to 8mm and put into a reaction vessel for biological enzymatic hydrolysis at 50℃. Alkaline enzyme, neutral enzyme and acidic enzyme (mass ratio 2:3:2) are added, with a total addition amount of 0.8wt% of the animal raw material mass. At the same time, 2.5wt% of mineral-derived potassium humate is added. The rotation speed is 70r / min, and the enzymatic hydrolysis is carried out for 10h. After the enzymatic hydrolysis is completed, it is sterilized by keeping it at 121℃ for 30min.

[0041] S2. Fur rendering: After removing impurities from the animal fur, it is sent to a rendering furnace and rendered at 138℃ and 0.15MPa for 2.5 hours to obtain crude protein (crude protein content 82%).

[0042] S3. Mixed Fermentation: Mix peanut oil residue, bones (20 mesh), eggshells (30 mesh) with S2 crude protein in a mass ratio of 3:2:1:2, then mix with minerals (stone: pearl stone: jade stone: agate stone fragments: zeolite = 2:2:1:1:4, 80 mesh) in a mass ratio of 10:4, grind to 80 mesh, send to a fermentation tank, ferment at 32℃ for 18 days, turning twice a day.

[0043] S4. Additive adjustment for fermentation: Add auxiliary ingredients (MSG factory scraps: syrup: bacteria = 4:3:1), the amount added is 6wt% of the mass of the mixture in S3, and at the same time add 1.5wt% of mineral potassium humate, adjust the pH to 7.0, continue fermentation at 32℃ for 6 days, and supplement 2.5wt% of minerals and 4wt% of macro and micro elements.

[0044] S5. Grinding and sieving: Mix the fermented material with the amino acids and meat juice (1:1) from S1 at a mass ratio of 10:1.2. Grind the material in a ball mill at 350 r / min for 2.5 h to 200 mesh. Then, sieve the material through a double-layer vibrating screen. The material on the screen is returned to the grinding mill.

[0045] S6. First cooking: Send the sieved material into the cooking tank, add 0.4wt% of compound enzyme (alkaline enzyme: neutral enzyme = 1:1), cook at 48℃ and 50r / min for 8 days, and purify with oxygen for 1 hour each day.

[0046] S7. Secondary boiling and aging: Add 0.25wt% acidic enzyme, 0.8wt% citric acid, 8wt% 300-mesh ore powder and 4wt% MSG factory and food factory scraps (1:1), boil at 42℃ and 40r / min for 12 days; age at 28℃ and 65% relative humidity for 18 days to obtain the finished product.

[0047] Finished product specifications: Brownish-brown powder, moisture 11%, pH 7.0, organic matter content 48%, small molecule amino acid content 9%, effective viable bacteria count 1.5 × 10⁻⁶ 6 CFU / g, fineness 200 mesh.

[0048] Example 3 A method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent includes the following steps: S1. Enzymatic hydrolysis of animal raw materials: Slaughterhouse waste and dead livestock and poultry are crushed to 10mm and put into a reaction vessel for biological enzymatic hydrolysis at 52℃. Alkaline enzyme, neutral enzyme and acidic enzyme (mass ratio 2:3:2) are added, with a total addition amount of 1.0wt% of the animal raw material mass. At the same time, 3wt% of mineral-derived potassium humate of animal raw material mass is added. The rotation speed is 80r / min, and the enzymatic hydrolysis is carried out for 12h. After the enzymatic hydrolysis is completed, it is sterilized by keeping it at 121℃ for 30min.

[0049] S2. Fur rendering: After removing impurities from the animal fur, it is sent to a rendering furnace and rendered at 143℃ and 0.15MPa for 3 hours to obtain crude protein (crude protein content 85%).

[0050] S3. Mixed Fermentation: Mix cottonseed oil residue, bones (20 mesh), eggshells (30 mesh) with S2 crude protein in a mass ratio of 3:2:1:2, then mix with minerals (stone: pearl stone: jade stone: agate stone fragments: zeolite = 2:2:1:1:4, 100 mesh) in a mass ratio of 10:5, grind to 80 mesh, send to a fermentation tank, ferment at 35℃ for 20 days, turning twice a day.

[0051] S4. Additive adjustment for fermentation: Add auxiliary ingredients (MSG factory scraps: syrup: bacteria = 4:3:1), the amount added is 8wt% of the mass of the mixture in S3, and at the same time add 2wt% of mineral potassium humate, adjust the pH to 7.5, continue fermentation at 35℃ for 8 days, and supplement 3wt% of minerals and 5wt% of macro and micro elements.

[0052] S5. Grinding and sieving: Mix the fermented material with the amino acids and meat juice (1:1) from S1 at a mass ratio of 10:1.5. Grind the material in a ball mill at 400 r / min for 3 hours to 300 mesh. Then, sieve the mixture through a double-layer vibrating screen. The material on the screen is returned to the grinding mill.

[0053] S6 First-time boiling: Send the sieved material into the boiling tank, add 0.5wt% of compound enzyme (alkaline enzyme: neutral enzyme = 1:1), boil at 50℃ and 50r / min for 10 days, and purify with oxygen for 1 hour daily.

[0054] S7 Secondary Boiling and Aging: Add 0.3wt% acidic enzyme, 1.0wt% citric acid, 10wt% 300-mesh ore powder and 5wt% MSG factory and food factory scraps (1:1), boil at 45℃ and 40r / min for 15 days; age at 30℃ and 70% relative humidity for 20 days to obtain the finished product.

[0055] Finished product specifications: Brownish-brown powder, moisture 12%, pH 7.5, organic matter content 50%, small molecule amino acid content 10%, effective viable bacteria count 2.0 × 10⁻⁶ 6 CFU / g, fineness 300 mesh.

[0056] Experimental Example 1: Comparative test of soil improvement effects I. Experimental Objective The effects of the soil remediation agent of this invention on improving soil organic matter content, bulk density (degree of compaction), oxygen content and salinization degree were verified, and compared with the remediation efficacy of traditional organic amendments.

[0057] II. Preparation of Test Samples 1. Sample of the present invention: Bio-enzymatic hydrolysis of mineral oxygen-supplying soil remediation agent prepared according to Example 2.

[0058] 2. Comparison sample: Commercially available traditional organic soil amendment (40% organic matter content, the main components are decomposed straw and sheep manure).

[0059] III. Experimental Procedures and Methods 1. Experimental soil: Compacted and saline farmland soil was selected. Initial indicators: organic matter content 0.8%, bulk density 1.5 g / cm³. 3 Oxygen content 7%, EC value 2.5 mS / cm.

[0060] 2. Experimental Design: Three treatment groups were set up, with three replicates in each group, and the plot area was 20m². 2 Randomized block permutation: Control group: No amendments were applied, and conventional fertilization (chemical fertilizer) was used.

[0061] Control group: Traditional organic soil amendment was applied at a rate of 300 kg per acre, combined with tillage and conventional fertilization.

[0062] Experimental group: The remediation agent of this invention was applied at a rate of 200 kg per mu (approximately 0.067 hectares), combined with tillage, and conventional fertilization was carried out (chemical fertilizer usage reduced by 20%). 3. Cultivation and testing: Corn was planted, with a growth period of 120 days. Soil indicators were tested after harvest. Organic matter content: potassium dichromate titration method.

[0063] Soil bulk density: ring cutter method.

[0064] Soil oxygen content: Soil oxygen sensor method.

[0065] EC value: water-to-soil ratio 1:5, measured by conductivity meter.

[0066] IV. Experimental Data and Results V. Experimental Conclusions 1. The remediation agent of this invention has a significant effect on improving soil organic matter, with an improvement rate of 47.5%, which is higher than the 37.5% of traditional soil amendments, and the dosage is less (200 kg / mu vs 300 kg / mu).

[0067] 2. The product has a significant effect on improving soil compaction, with a bulk density reduction rate of 16.67%, which is significantly better than traditional soil conditioners, indicating that the product can effectively reconstruct the soil aggregate structure.

[0068] 3. Excellent oxygen supply and salinization improvement effects: soil oxygen content increased to 13.2% and EC value decreased by 40%, which was far better than the control group and the comparison group, verifying the oxygen supply and desalination effect of the porous structure of the ore.

[0069] 4. The experimental group achieved a better repair effect than the control group even with a 20% reduction in fertilizer usage, demonstrating the product's advantages in saving fertilizer and increasing efficiency.

[0070] Experiment Example 2: Verification Experiment on the Oxygen Supply Effect of Ore during Fermentation I. Experimental Objective: To verify the oxygen supply role of minerals (stone, pearlite, jade, agate fragments, zeolite) during fermentation, and to compare the effects of adding or not adding minerals on the fermentation cycle and the degree of material decomposition.

[0071] II. Preparation of Test Samples 1. Experimental group: Minerals (4wt%) were added according to the S3-S4 formula of Example 2.

[0072] 2. Control group: Except for the absence of added minerals, the other formulas and process parameters were completely identical to those of the experimental group.

[0073] III. Experimental Procedures and Methods 1. Fermentation process monitoring: The oxygen concentration in the fermentation system was measured (oxygen sensor) on days 5, 10, 15 and 18 of fermentation, and the fermentation cycle (the time until the material maturity is ≥90%) was recorded.

[0074] 2. Decomposition degree test: The germination index (GI) method was used to determine the germination index of cucumber seeds by the extract of fermented material. A GI of ≥80% was considered as complete decomposition.

[0075] 3. Index testing: After fermentation, the crude protein content and amino acid nitrogen content of the material are tested.

[0076] IV. Experimental Data and Results V. Experimental Conclusions 1. The oxygen concentration in the fermentation system of the experimental group was consistently higher than that of the control group, indicating that the porous structure of the minerals can continuously release oxygen and ensure an aerobic fermentation environment.

[0077] 2. The fermentation cycle of the experimental group was only 18 days, which was 10 days shorter than that of the control group, and the fermentation efficiency was increased by 35.7%, which verified the accelerating effect of ore oxygen supply on the fermentation process.

[0078] 3. The maturity (GI=85.6%), crude protein content, and amino acid nitrogen content of the experimental group were all higher than those of the control group, indicating that the aerobic fermentation was more complete and the material conversion efficiency was higher.

[0079] Experiment Example 3: Production Continuity and Efficiency Experiment I. Experimental Objective The production efficiency and product quality uniformity of the "continuous fermentation + automated grinding and boiling" production process of this invention are compared with those of the traditional "batch fermentation + manual operation" process.

[0080] II. Experimental Design and Equipment 1. Experimental group: The industrial production line of this invention is adopted, including automated crushing equipment, continuous fermentation tank, ball mill, automatic boiling system, aging chamber, and a supporting PLC control system.

[0081] 2. Control group: Traditional batch production line, including manual feeding crusher, batch fermentation tank, small grinder, and manual mixing equipment.

[0082] III. Experimental Procedures and Methods 1. Production Operation: Both processes run continuously for 8 hours, recording the output of qualified products, labor consumption, and energy consumption.

[0083] 2. Quality uniformity test: During the production process, the experimental group was sampled once every 2 hours, and the control group was sampled once per batch, for a total of 4 samples, and the coefficient of variation (CV%) of organic matter content, small molecule amino acid content and fineness was tested.

[0084] 3. Calculation indicators: production efficiency (kg / h), raw material utilization rate (%), and labor cost (yuan / kg).

[0085] IV. Experimental Data and Results V. Experimental Conclusions 1. The continuous production process of this invention has a production efficiency of 150 kg / h, which is 8 times that of the traditional process, and the output in 8 hours reaches 1200 kg, which significantly improves the industrial production capacity.

[0086] 2. The raw material utilization rate reaches 95%, which is higher than the 82% of the traditional process, reducing resource waste and lowering production costs.

[0087] 3. The product has excellent quality uniformity, with the coefficient of variation of all indicators being less than 5%, which is far superior to traditional processes (CV%>9%), ensuring stable product application effects.

[0088] 4. The labor cost is only 0.2 yuan / kg, which is 86.7% lower than the traditional process. It has a high degree of automation and is suitable for large-scale industrial production.

[0089] Experiment Example 4: Experiment on the Application Effect in Crop Planting I. Purpose of the experiment: To verify the application effect of the soil remediation agent of the present invention in crop planting, including yield increase, stress resistance enhancement and pesticide reduction effect.

[0090] II. Test Samples and Crops 1. Test sample: Bio-enzymatic hydrolysis of mineral oxygen-supplying soil remediation agent prepared in Example 2.

[0091] 2. Experimental crops: maize (variety: Zhengdan 958) and tomato (variety: Zhongza 105).

[0092] III. Experimental Procedures and Methods 1. Experimental Design: Three treatment groups were set up, with three replicates in each group, and the plot area was 20m². 2 Random arrangement: Control group: conventional fertilization (chemical fertilizer) + conventional pesticide control.

[0093] Control group: conventional fertilization + conventional pesticides + traditional organic soil amendment (300 kg / mu).

[0094] Experimental group: conventional fertilization (reduced by 20%) + pesticide (reduced by 30%) + the repair agent of this invention (200 kg / mu).

[0095] 2. Planting Management: Conduct field management according to conventional agricultural technical procedures, and record the growth period, disease occurrence, and yield of corn and tomatoes.

[0096] 3. Testing indicators: yield per mu, disease incidence, stress resistance (drought resistance days), and fruit quality (tomato vitamin C content).

[0097] IV. Experimental Data and Results V. Experimental Conclusions 1. The repair agent of this invention can significantly increase crop yield, with corn yield reaching 750 kg per mu, an increase of 15.38%; and tomato yield reaching 5200 kg per mu, an increase of 15.56%, which is superior to traditional organic soil amendments.

[0098] 2. Crops have enhanced resilience, with corn able to withstand drought for up to 14 days, the incidence of late blight in tomatoes reduced to 4%, pesticide use reduced by 30%, and environmental pollution reduced.

[0099] 3. Improved fruit quality: The vitamin C content of tomatoes reached 32.1 mg / 100g, an increase of 26.9% compared with the control group, achieving a win-win situation of increased yield and improved quality.

[0100] The above description has been given for purposes of illustration and description. Furthermore, this description is not intended to limit the embodiments of this disclosure to the forms disclosed herein. Although numerous exemplary aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, alterations, additions, and sub-combinations therein.

Claims

1. A method for preparing a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent, characterized in that, Includes the following steps: S1 Animal Raw Material Enzymatic Hydrolysis: Slaughterhouse by-products and dead poultry, pigs, cattle, etc. from pollution-free treatment plants are first crushed, and then biologically enzymatically hydrolyzed in a reaction vessel at a low temperature of about 50 degrees Celsius. Alkaline enzymes, neutral enzymes, and acidic enzymes are used, and mineral-derived potassium humate is added on the basis of enzymatic hydrolysis to decompose them completely into small molecule amino acids, and then high-temperature sterilization is carried out. S2 Fur Processing: Animal fur is processed into crude protein by passing it through a chemical furnace at a high temperature of 138 degrees Celsius. S3 Mixed Fermentation: Vegetable oil residue (peanut, sunflower seed, cotton, soybean, etc.), bones, eggshells, and well-digested crude protein, kitchen waste (bitter bean, walnut husk) and minerals are mixed together in a certain proportion, crushed, and then fermented; the minerals include zeolite, pearlite, jade, agate fragments, and zeolite. S4 auxiliary material regulation fermentation: Based on S3, add auxiliary materials, such as MSG factory scraps, syrup and bacteria, and add mineral-derived potassium humate to adjust the pH value and carbon neutralize, and ferment together, while increasing minerals and macro- and micro-elements; S5 Grinding and Sieving: Grind all the fermented materials together without adding water. Use enzymatically hydrolyzed amino acids and meat broth to grind them into a mesh size of 150-300 mesh or higher using a ball mill, and then sieve them. S6 One-time Boiling: The sieved material is boiled together through biological enzymatic hydrolysis and fermentation. S7 Secondary Boiling and Aging: Acidic enzymes, citric acid, mineral powder, and a small amount of scraps from MSG and food factories are added for secondary biological enzymatic fermentation and boiling, followed by aging.

2. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S1, the temperature of the bio-enzymatic hydrolysis is controlled at around 50°C, and the enzymes used include alkaline enzymes, neutral enzymes, and acidic enzymes; potassium humate from minerals is added during the enzymatic hydrolysis process to promote the decomposition of materials into small molecule amino acids.

3. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S2, the animal fur is treated in a chemical furnace at a temperature of 138 degrees Celsius, and the treatment product is crude protein.

4. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S3, the vegetable oil residue includes peanut, sunflower seed, cotton, and soybean oil residue; the minerals include zeolite, pearlite, jade, agate fragments, and zeolite; the materials are mixed in proportion, crushed, and then fermented.

5. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S4, the auxiliary materials include monosodium glutamate factory scraps, syrup, and bacteria; potassium humate is added to adjust the pH value and neutralize carbon; and minerals and macro- and micro-elements are added during the fermentation process.

6. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S5, no water is added during the grinding process; the enzymatically hydrolyzed amino acids and meat broth are used as the medium. The grinding equipment is a ball mill, and the grinding fineness is controlled at 150-300 mesh or higher.

7. The preparation method of a bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 1, characterized in that, In step S7, the additives for the secondary enzymatic hydrolysis, fermentation, and boiling process include acidic enzymes, citric acid, mineral powder, and a small amount of scraps from monosodium glutamate (MSG) factories and food factories.

8. A bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent prepared by the method according to any one of claims 1 to 7, characterized in that, The soil remediation agent is a material that has undergone biological enzymatic hydrolysis, fermentation, and grinding and mixing. Several minerals in the material play a role in supplying oxygen to the material, enabling the material to undergo aerobic fermentation during the preparation process.

9. The bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 8, characterized in that, The soil remediation agent is used to improve crop resistance, increase yield, increase organic matter, increase soil oxygen content, remove soil compaction and salinization, and reduce the use of pesticides and industrial fertilizers.

10. The application of the bio-enzymatic hydrolysis mineral oxygen-supplying soil remediation agent according to claim 8 or 9 in crop cultivation, characterized in that, It can be applied to the planting of various crops, fruits and vegetables, cotton, corn and soybeans. The fertilization methods include root application, foliar application and drip irrigation.

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