An indazole amide-containing insecticidal composition and use thereof

Abstract

The application belongs to the technical field of pesticide insecticide, and discloses an indazolic amide-containing insecticidal composition and application thereof. The insecticidal composition comprises active ingredient A and active ingredient B. The active ingredient A is indazolic amide, and the active ingredient B is any one of benzpyrimoxan, flupyrimin and triflumezopyrim. The mass ratio of the active ingredient A to the active ingredient B is 50:1-1:50. The insecticidal composition can significantly improve the control effect, the action mechanism is complementary, the insect pests can be quickly knocked down and the effective period is prolonged, the aphid population with resistance can be efficiently controlled, the development of single pesticide resistance can be delayed through compounding, the resistance risk is reduced, the control effect can be achieved at a low dose, and pesticide residues and environmental pollution are reduced.

Description

[0001] This invention application is a divisional application of application number 202510822995.8, filed on June 19, 2025, entitled "An insecticidal composition containing indazole and its application". Technical Field

[0002] This invention belongs to the field of pesticide insecticide technology, and discloses an insecticidal composition containing indazole and its application. Background Technology

[0003] Aphids (Aphidoidea) are a widespread group of piercing-sucking pests that damage crops, can transmit plant viruses, and lead to reduced yields or even crop failure. Traditional control methods rely on chemical insecticides, but long-term use of single pesticides has led to increased aphid resistance (e.g., the frequent emergence of populations resistant to imidacloprid and thiamethoxam), and some pesticides have high toxicity to non-target organisms and significant environmental residue problems. Therefore, the development of highly effective, low-toxicity, low-residue compound insecticides that can delay the development of resistance has become a research hotspot.

[0004] Indazole, belonging to the indoleamide class of insecticides, is an insecticide with a unique 2-H indole skeleton structure. Its novel structure and unique mechanism of action make it highly effective against piercing-sucking pests such as whiteflies, thrips, leafhoppers, and cotton aphids. Its structural formula is shown below: .

[0005] benzpyrimoxan is a novel insecticide with an arylalkoxypyrimidine structure developed by Nippon Nippon Agricultural Chemicals Co., Ltd. It has a unique chemical structure and a novel mechanism of action. It can reduce the insect population in rice fields by inhibiting the molting of planthopper and leafhopper larvae, and can effectively control rice planthoppers, leafhoppers and resistant rice planthoppers.

[0006] Flupyrimin is an insecticide developed by Meiji Seika Pharmaceutical Co., Ltd. of Japan that acts on the nicotinic acetylcholine receptor (nAChR). Unlike most insecticides that act on the nicotinic acetylcholine receptor, it is an antagonist, relatively safe for pollinating insects, and effective against a variety of pests, including Hemiptera, Lepidoptera, Thysanoptera, and Diptera, as well as pests resistant to imidacloprid. It can be widely used in agricultural and non-agricultural fields.

[0007] Triflumezopyrim is a meso-ionic pyrimidinone insecticide mainly used on crops such as rice, corn, potatoes, cotton, and soybeans, as well as in greenhouse nurseries, to control lepidopteran and homoptera pests.

[0008] Currently, pest control primarily relies on pesticides that act on the insect nervous system (such as neonicotinoids and pyrethroids) and chitin synthesis inhibitors (such as dihydrazides). However, existing compound formulations have significant drawbacks: limited mechanisms of action (most formulations target only a single target, making them susceptible to resistance development due to target mutations); an imbalance between rapid action and duration of effect (some pesticides, while providing rapid knockdown, have short durations of effect, requiring frequent application); and insufficient environmental compatibility (highly toxic or persistent pesticides disrupt ecological balance, threatening pollinating insects and natural enemies). Therefore, there is an urgent need to develop a novel compound system that balances rapid knockdown, simultaneous control of insect eggs and pests, and delayed resistance development to meet the integrated pest management needs of modern agriculture. The applicant intends to study the control effects on target pests by rationally combining indazole with any one of benzpyrimoxan, flupyrimin, or triflumezopyrim. Existing research indicates that there are no reports of combining these three components (benzpyrimoxan, flupyrimin, and triflumezopyrim) with indazole. In particular, the complementary mechanisms of action and resistance control potential of these three components when used separately with indazole have not been fully explored. Summary of the Invention

[0009] Based on the above, this invention provides an insecticidal composition containing indazole amide, which overcomes the limited knockdown effect of indazole amide on adult insects when used alone. The active ingredients have complementary mechanisms of action, significantly improving the control effect, quickly knocking down pests and prolonging the residual effect. It has a highly effective control capability against aphid populations that have developed resistance. By combining the two, the development of resistance to single agents can be delayed, reducing the risk of resistance. The control effect can be achieved with low dosage, reducing pesticide residues and environmental pollution.

[0010] To achieve the above objectives, the present invention adopts the following technical solution: an insecticidal composition containing indazole amide and its application, wherein the insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is indazole amide, and active ingredient B is any one of benzpyrimoxan, flupyrimin, and triflumezopyrim, and the mass ratio of active ingredient A to active ingredient B is 50:1 to 1:50, or any value within the above ratio range.

[0011] Furthermore, the active ingredient B is benzpyrimoxan, and the mass ratio of active ingredient A to active ingredient B is 40:1 to 1:30, or any value within the above ratio range.

[0012] The active ingredient B is flupyrimin, and the mass ratio of active ingredient A to active ingredient B is 32:1 to 1:20, or any value within the above range.

[0013] The active ingredient B is triflumezopyrim, and the mass ratio of active ingredient A to active ingredient B is 30:1 to 1:20, or any value within the above range.

[0014] Furthermore, the active ingredient B is benzpyrimoxan, and the mass ratio of active ingredient A to active ingredient B is 30:1 to 1:30, or any value within the above ratio range.

[0015] The active ingredient B is flupyrimin, and the mass ratio of active ingredient A to active ingredient B is 20:1 to 1:20, or any value within the above range.

[0016] The active ingredient B is triflumezopyrim, and the mass ratio of active ingredient A to active ingredient B is 16:1 to 1:10, or any value within the above range.

[0017] Furthermore, the active ingredient B is benzpyrimoxan, and the mass ratio of active ingredient A to active ingredient B is 20:1 to 1:10, or any value within the above ratio range.

[0018] The active ingredient B is flupyrimin, and the mass ratio of active ingredient A to active ingredient B is 12:1 to 1:6, or any value within the above range.

[0019] The active ingredient B is triflumezopyrim, and the mass ratio of active ingredient A to active ingredient B is 8:1 to 3:2, or any value within the above range.

[0020] Furthermore, based on a total weight of 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 0.01% to 80% of the total weight of the insecticidal composition.

[0021] Furthermore, in addition to the active ingredient, the insecticidal composition also contains pesticide-permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0022] Furthermore, the insecticidal composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation.

[0023] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder; the liquid formulation is an emulsifiable concentrate, a suspension concentrate, a microemulsion, a dispersible oil suspension, or a water emulsion.

[0024] Furthermore, the present invention also discloses the application of the insecticidal composition described above for the control of agricultural, horticultural or forestry pests, characterized in that the pest is an aphid.

[0025] Furthermore, the insecticidal composition or its formulation is applied to the pest that needs to be controlled or the medium in which it grows.

[0026] To achieve the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application location, application method, and formulation used.

[0027] The beneficial effects of this invention are as follows: 1) The active ingredients in the insecticidal composition of the present invention have complementary mechanisms of action, which significantly improves the control effect on target pests and has a significant synergistic effect.

[0028] 2) The insecticidal composition of the present invention can quickly knock down pests and prolong the duration of effectiveness, and has a high-efficiency control capability against aphid populations that have developed resistance.

[0029] 3) The insecticidal composition of the present invention delays the development of resistance to single agents by compounding, reduces the resistance defense line, and can achieve satisfactory control effect with low dosage, thereby reducing pesticide residues and environmental pollution. Detailed Implementation

[0030] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0031] Preparation method of formulation.

[0032] 1. Suspension preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0033] 2. Preparation method of emulsifiable concentrate: According to the formula ratio, add the measured active ingredients, solvent and co-solvent into the mixing tank and stir to dissolve them. Then add the emulsifier, and use the remaining solvent to make up the balance. Stir evenly in the mixing tank, and filter to obtain the emulsifiable concentrate required by the present invention.

[0034] 3. Preparation method of dispersible oil suspension: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0035] 4. Preparation method of water-dispersible granules: According to the formulation ratio of the example, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0036] 5. Preparation method of wettable powder: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a mixer. After being pulverized by an air jet mill, they are mixed evenly again to prepare the wettable powder of the composition of the present invention.

[0037] 6. Microemulsion preparation method: According to the formulation ratio in the example, the active ingredients, solvent, emulsifier, etc. are mixed evenly to obtain the oil phase. The antifreeze and water are mixed evenly to obtain the aqueous phase. The oil phase is added to the aqueous phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, the defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01~0.1 micrometers, which is the microemulsion product.

[0038] 7. Preparation method of water-emulsion: According to the formulation ratio in the example, dissolve the active ingredient in the solvent and add the emulsifier to dissolve it into a uniform oil phase. Mix deionized water, antifreeze, etc. together to form a uniform aqueous phase. Under high-speed shearing, add the oil phase to the aqueous phase and shear until the particle size is qualified. Then add defoamer, thickener, and preservative and stir evenly to form a well-dispersed water-emulsion product.

[0039] Formulation preparation example: Preparation Example 1: 20% Indoxam·benzpyrimoxan suspension concentrate (1:1) Formula composition: 10% indazole, 10% benzpyrimoxan, 1% sodium dodecyl sulfate, 1% naphthalene sulfonate formaldehyde condensate, 3% styrene phenol polyoxyethylene ether phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, deionized water to make up the balance.

[0040] Preparation Example 2: 26% Indoxam·flupyrimin Suspension (12:1) Formula composition: 24% indazole, 2% flupyrimin, 0.5% alkylphenol polyoxyethylene ether, 4% alkylphenol polyoxyethylene ether phosphate, 1% sodium lignosulfonate, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0041] Preparation Example 3: 20% Indoxam·triflumezopyrim suspension (3:2) Formula composition: 12% indazole, 8% triflumezopyrim, 1% fatty alcohol polyoxyethylene ether, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% styrene phenol polyoxyethylene ether sulfate, 1% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance.

[0042] Preparation Example 4: 12% Indoxam·benzpyrimoxan EC (1:5) Formula composition: 2% indazole, 10% benzpyrimoxan, 15% DMF, 14% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% propylene carbonate, xylene to make up the balance.

[0043] Preparation Example 5: 14% Indoxam·flupyrimin EC (6:1) Formula composition: 12% indazole, 2% flupyrimin, 10% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% sodium lignosulfonate, 12% DMF, 15% N-methylpyrrolidone, and methyl oleate to make up the balance.

[0044] Preparation Example 6: 9% Indoxam·triflumezopyrim EC (8:1) Formulation composition: 8% indazole, 1% triflumezopyrim, 15% acetophenone, 12% N-octylpyrrolidone, 2% sodium dodecyl sulfate, 12% EO / PO block copolymer, and trimethylbenzene to make up the balance.

[0045] Preparation Example 7: 11% Indoxam·benzpyrimoxan Dispersible Oil Suspension (1:10) Formula composition: 1% indazole nitrile, 10% benzpyrimoxan, 3% naphthalene sulfonate formaldehyde condensate, 14% isotridecyl alcohol polyoxyethylene ether, 5% tristyrene phenol polyoxyethylene ether, 2% sodium lignosulfonate, 1% silica, 1% organobentonite, 18% 200# solvent oil, methyl oleate to make up the balance.

[0046] Preparation Example 8: 20% Indoxam·flupyrimin Dispersible Oil Suspension (3:1) Formula composition: 15% indazole, 5% flupyrimin, 5% glycerol fatty acid ester polyoxyethylene ether, 10% fatty amine polyoxyethylene ether, 2% sodium lignosulfonate, 1% sodium polycarboxylate, 2% naphthalene sulfonate formaldehyde condensate, soybean oil to make up the balance.

[0047] Preparation Example 9: 12% Indoxam·triflumezopyrim Dispersible Oil Suspension (1:5) Formula composition: 2% indazole, 10% triflumezopyrim, 2% phenylethylphenol polyoxyethylene polyoxypropylene ether, 12% arylphenol polyoxyethylene ether phosphate, 2% calcium dodecylbenzenesulfonate, 3% sodium polycarboxylate, 2.5% magnesium aluminum silicate, 1.5% Tesco 869, corn oil to make up the balance.

[0048] Preparation Example 10: 24% Indoxam·benzpyrimoxan water-dispersible granules (5:1) Formula composition: 20% indazole, 4% benzpyrimoxan, 12% naphthalene sulfonate formaldehyde condensate, 5% sodium dodecylbenzene sulfonate, 3% sodium dodecyl sulfate, 5% silica, 25% starch, kaolin to make up the balance.

[0049] Preparation Example 11: 28% Indoxam·flupyrimin water-dispersible granules (1:6) Formula composition: 4% indoxam, 24% flupyrimin, 15% naphthalene sulfonate, 2.5% BX (a type of chemical additive), 2% calcium dodecylbenzenesulfonate, 5% white sugar, and kaolin to make up the balance.

[0050] Preparation Example 12: 22% Indoxam·triflumezopyrim water-dispersible granules (1:10) Formula composition: 2% indazole, 20% triflumezopyrim, 10% calcium dodecylbenzenesulfonate, 1% naphthalenesulfonate formaldehyde condensate, 3% sodium dodecyl sulfate, 10% ammonium sulfate, starch to make up the balance.

[0051] Preparation Example 13: 33% Indoxam·benzpyrimoxan wettable powder (10:1) Formula composition: 30% indazole, 3% benzpyrimoxan, 5% sodium dodecyl sulfate, 5% sodium alkyl polyoxyethylene ether sulfonate, 2.5% BX (a type of chemical additive), 5% silica, and kaolin to make up the balance.

[0052] Preparation Example 14: 26% Indoxam·flupyrimin wettable powder (1:12) Formula composition: 2% indazole, 24% flupyrimin, 3% sodium dodecyl sulfate, 3% succinate sulfonate, 5% sodium alkylnaphthalene sulfonate, 10% kaolin, 12% silica, and bentonite to make up the balance.

[0053] Preparation Example 15: 21% Indoxam·triflumezopyrim wettable powder (6:1) Formula composition: 18% indazole, 3% triflumezopyrim, 2% sodium polynaphthalene sulfonate, 2% succinate sulfonate, 3% stretching powder BX, 5% silica, and kaolin to make up the balance.

[0054] Preparation Example 16: 16% Indoxam·benzpyrimoxan microemulsion (1:3) Formula composition: 4% indazole, 12% benzpyrimoxan, 20% cyclohexanone, 14% isotridecyl alcohol polyoxyethylene ether, 2% ethylene glycol oxyethylene polyoxypropylene ether, 2% sodium alkyl polyoxyethylene ether sulfonate, 0.25% silicone defoamer, deionized water to make up the balance.

[0055] Preparation Example 17: 9% Indoxam·flupyrimin microemulsion (1:8) Formula composition: 1% indazole, 8% flupyrimin, 12% xylene, 15% cyclohexanone, 12% isotridecyl alcohol polyoxyethylene ether, 5% EO-PO block copolymer, 1% styrene-phenol polyoxyethylene ether sulfate, 5% glycerol, 0.05% silicone defoamer, deionized water to make up the balance.

[0056] Preparation Example 18: 10% indoxoropyram·triflumezopyrim microemulsion (1:1) Formula composition: 5% indazole, 5% triflumezopyrim, 10% xylene, 20% cyclohexanone, 15% glycerol fatty acid ester polyoxyethylene ether, 3% EO-PO block copolymer, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 4% ethylene glycol, 0.1% silicone defoamer, deionized water to make up the balance.

[0057] Preparation Example 19: 14% Indoxam·benzpyrimoxan water emulsion (6:1) Formula composition: 12% indazole, 2% benzpyrimoxan, 5% ethylene glycol oxyethylene polyoxypropylene ether, 4% fatty alcohol polyoxyethylene ether phosphate, 15% cyclohexanone, 0.25% xanthan gum, 0.2% potassium benzoate, 5% glycerol, 2% urea, 1.5% sodium sorbate, 0.1% silicone defoamer, deionized water to make up the balance.

[0058] Preparation Example 20: 16% Indoxam·flupyrimin water emulsion (7:1) Formula composition: 14% indazole, 2% flupyrimin, 5% xylene, 15% cyclohexanone, 2% polyoxyethylene dehydrated sorbitan monooleate, 8% ethylene oxide-propylene oxide copolymer, 5% ethylene glycol, 0.1% silicone defoamer, 0.1% xanthan gum, 0.1% benzoic acid, deionized water to make up the balance.

[0059] Preparation Example 21: 9% Indoxam·triflumezopyrim water emulsion (5:4) Formula composition: 5% indazole, 4% triflumezopyrim, 10% triphenylethylphenol polyoxyethylene ether, 2% alkylphenol formaldehyde resin polyoxyethylene ether sulfate, 15% cyclohexanone, 0.25% xanthan gum, 5% ethylene glycol, 0.15% sodium sorbate, 0.5% silicone oil, deionized water to make up the balance.

[0060] Example 1: Indoor aphid bioactivity test.

[0061] Test reagents: Indazole, benzpyrimoxan, flupyrimin, and triflumezopyrim technical grade pesticides. Based on the preliminary test results, five series concentrations were set up using a proportional method in the formal test. The technical grade pesticides were dissolved and diluted with a suitable solvent to prepare a stock solution of a certain concentration. Before the test, the stock solution was further diluted proportionally with a 0.1% (v / v) Tween-80 aqueous solution.

[0062] The insect source used for testing was alfalfa aphids (nymphs).

[0063] Experimental Method: Leaf-disc spraying method. Filter paper was placed in a 9cm diameter petri dish, and an appropriate amount of distilled water was added to moisten the filter paper, but without standing water. Air between the filter paper and the petri dish was removed, ensuring the filter paper adhered tightly to the bottom of the dish. A moistened cotton ball was then placed in the dish to maintain humidity. Alfalfa leaves were punched into leaf discs, with two leaf discs placed in each dish. Selected alfalfa aphids of uniform growth, raised indoors, were introduced onto the leaf discs, 30 aphids per dish, with each dish constituting one replicate. Each concentration treatment was replicated four times. During treatment, the petri dishes were placed in the bottom tray of a Potter spray tower for spraying, with a spray volume of 1mL. After the solution settled for 1 minute, it was removed. The control was treated with the same amount of water. The petri dishes were incubated at (25±1)℃, relative humidity of 70%±5%, and a light-dark cycle of 16h:8h. After 48 hours, the mortality rate was checked. The insects were gently touched with the tip of a brush; those that did not move or were stiff were considered dead.

[0064] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0065] In the formula: P — Mortality rate, expressed as a percentage (%); K —This indicates the number of dead insects, expressed in heads; N — This indicates the total number of insects treated, in units of heads.

[0066]

[0067] In the formula: P 1 —Adjusted mortality rate, in percentages (%); P t —The mortality rate is expressed as a percentage (%). P 0 — Mortality rate in blank control group, expressed as a percentage (%).

[0068] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0069] The toxicity regression equation, correlation coefficient, and LC were obtained using a statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0070] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0071] In the formula: YOU —Measured toxicity index of the mixture; S LC of standard insecticides 50 The unit is milligrams per liter (mg / L); M LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0072]

[0073] In the formula: TTI —Theoretical toxicity index of the mixture; THE A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). THE B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0074]

[0075] In the formula: CTC —Cotoxicity coefficient; YOU —Measured toxicity index of the mixture; TTI —Theoretical toxicity index of mixed preparations.

[0076] Co-toxicity coefficient of compound CTC ≥120 exhibits a synergistic effect; CTC≤80 It exhibits antagonistic effects; 80 < CTC <120 exhibits an additive effect.

[0077]

[0078] As shown in Table 1, the combination of indazole and benzpyrimoxan in an appropriate ratio exhibits good activity against alfalfa aphids. When the mass ratio of indazole to benzpyrimoxan is 1:30–40:1, the co-toxicity coefficient against alfalfa aphids is greater than 120, indicating a synergistic effect; when the mass ratio is 1:30–30:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect; when the mass ratio is 1:10–20:1, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect; the highest co-toxicity coefficient (219.375) is achieved at a mass ratio of 1:1; while at a mass ratio of 1:40, the co-toxicity coefficient is less than 120, indicating an additive effect.

[0079]

[0080] As shown in Table 2, the combination of indazole and flupyrimin in appropriate ratios exhibited good activity against alfalfa aphids. The co-toxicity coefficient of indazole and flupyrimin at a mass ratio of 32:1 to 1:20 was greater than 120, indicating a synergistic effect; at a mass ratio of 20:1 to 1:20, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; at a mass ratio of 12:1 to 1:6, the co-toxicity coefficient was greater than 140, showing a significant synergistic effect; the highest co-toxicity coefficient (194.320) was observed at a mass ratio of 3:1; while the co-toxicity coefficients at mass ratios of 1:50, 1:32, and 50:1 were less than 120, indicating an additive effect.

[0081]

[0082] As shown in Table 3, the combination of indazole and triflumezopyrim in appropriate ratios exhibited good activity against alfalfa aphids. When the mass ratio of indazole to triflumezopyrim was 30:1 to 1:20, the co-toxicity coefficient against alfalfa aphids was greater than 120, indicating a synergistic effect; when the mass ratio was 16:1 to 1:10, the co-toxicity coefficient against alfalfa aphids was greater than 130, showing a significant synergistic effect; when the mass ratio was 8:1 to 3:2, the co-toxicity coefficient against alfalfa aphids was greater than 140, showing a significant synergistic effect; the co-toxicity coefficient was highest at a mass ratio of 3:2, reaching 172.288; while the co-toxicity coefficients at mass ratios of 1:30, 1:40, and 45:1 were less than 120, indicating an additive effect.

[0083] Example 2: Field efficacy test for controlling radish aphids.

[0084] Target species: Radish aphid.

[0085] Experimental site: The experiment was conducted in Caiyuan Village, Zhangshanzi Town, Taierzhuang District, Zaozhuang City, Shandong Province. The experimental site is flat, and the soil is sandy loam with moderate fertility.

[0086] Experimental crop: rapeseed.

[0087] Experimental Design: The experiment included 6 treatments: 5 drug treatments and 1 control. All experimental plots were randomly assigned to block design, with 4 replicates per treatment. The plot area was 15 m². 2 The blocks are arranged in a randomized manner, and the cultivation and management conditions are consistent across all blocks.

[0088] Experimental Method: The experiment was conducted on August 15, 2024, with a single application of the pesticide. The tested pesticide was diluted to the standard field concentration and uniformly sprayed onto each plot using a SX-MD15D backpack electric sprayer. During the survey, five sampling points were established in each plot using a zigzag five-point sampling method, with 10 plants per point and two leaves per plant. A baseline aphid population was surveyed before application, and the number of surviving aphids was assessed at 1, 3, and 7 days after application, along with any pesticide damage.

[0089] Methods for calculating drug efficacy:

[0090]

[0091] The experimental results are shown in the table below:

[0092] Field efficacy trials showed that the combination of indoxam and benzpyrimoxan in a reasonable ratio had excellent control effects on radish aphids. The compound formulation had good rapid action, long-lasting effect, and did not cause phytotoxicity to the target crop, making it safe for crops.

[0093] Example 3: Field efficacy test for controlling peach aphids.

[0094] Experimental site: Peach orchard in Xima Village, Danianchen Town, Huimin County, Binzhou City, Shandong Province. The experimental area is flat, with sandy loam soil and moderate soil fertility. The peach trees are growing at a relatively uniform rate, with a tree spacing of 3m × 4m and a tree age of 10 years. Field management is carried out according to conventional methods.

[0095] Target pest: Peach aphid.

[0096] Experimental Design: The experiment included 5 pesticide treatments and 1 water control, for a total of 6 treatments. Each treatment was replicated 4 times, and each replicate was a plot with 4 peach trees per plot. The plots were arranged in a randomized block design. A MATABI Super Green 16 sprayer with a nozzle diameter of 0.8 mm and an operating pressure of 2.0 Bar was used to spray evenly, ensuring that all leaves were evenly covered on both sides.

[0097] Experimental survey: The survey was conducted according to the "National Standard of the People's Republic of China: Field Efficacy Test Guidelines for Pesticides (I) Insecticides for the Control of Aphids on Fruit Trees" (GB / T 17980.9—2000). Two trees were surveyed per plot, and five infested shoots were fixed on each tree (east, south, west, north, and center). The number of live aphids on the top 10 leaves of each shoot was surveyed. The initial aphid population was surveyed before application, and the number of live aphids on each treatment marker was surveyed at 3, 7, and 14 days after application.

[0098] Methods for calculating drug efficacy:

[0099]

[0100] The experimental results are shown in the table below:

[0101] Field efficacy trials showed that, 3 days after application, the control efficacy of the compound formulation was above 85%, demonstrating good rapid-acting properties. 14 days after application, the control efficacy of the compound formulation was above 92%, with a long-lasting effect.

[0102] Example 4: Field efficacy test for controlling cotton aphids.

[0103] Experimental site: Cotton fields in Xianhe Town, Hekou District, Dongying City, Shandong Province. The experimental site has flat terrain and good water and fertilizer conditions.

[0104] Target pest: Cotton aphid.

[0105] Experimental Design: The experiment consisted of 6 treatments, each replicated 4 times, in a randomized block design. Each plot was 27 m². 2 .

[0106] Experimental method: The experiment was conducted on July 13th. Before the application of the pesticide, the cotton aphids were severely infested and no other insecticides were used for control. The Weishi WS-16 manual sprayer was used to spray the cotton plants and leaves evenly once.

[0107] Experimental survey: Five samples were taken from each plot, with two plants fixed at each point. Two leaves with aphids were fixed on each plant, and the number of aphids on the fixed leaves was investigated. The initial aphid population was assessed before pesticide application, and the number of live aphids was assessed 1, 3, and 7 days after application. While investigating the efficacy of the pesticide, the effects on the crop were also observed. The aphid population reduction rate and control effect were calculated based on the survey data.

[0108] Methods for calculating drug efficacy:

[0109]

[0110]

[0111] One day after application, the following preparations were prepared: Example 12: 22% indoximetriflumezopyrim water-dispersible granules (1:10); Example 21: 9% indoximetriflumezopyrim water-emulsion (5:4); and Example 3: 20% indoximetriflumezopyrim suspension (3:2) at 45 g / hm². 2 The control efficiencies of the treated formulations were 80.62%, 87.71%, and 90.49%, respectively, significantly higher than the single-agent control. Three days after application, the control efficiencies of the following formulations were significantly improved: Example 12: 22% indoximetriflumezopyrim water-dispersible granules (1:10); Example 21: 9% indoximetriflumezopyrim water-emulsion (5:4); and Example 3: 20% indoximetriflumezopyrim suspension (3:2), reaching 84.81%, 90.44%, and 95.50%, respectively. Seven days after application, the control efficiencies of the following formulations were significantly improved at 45 g / hm²: Example 12: 22% indoximetriflumezopyrim water-dispersible granules (1:10); Example 21: 9% indoximetriflumezopyrim water-emulsion (5:4); and Example 3: 20% indoximetriflumezopyrim suspension (3:2). 2 The control efficacy under the treatments was 89.64%, 94.43%, and 98.39%, respectively, and the three compound preparations all showed excellent sustained efficacy.

Claims

1. An insecticidal composition containing indazole and its application, characterized in that, The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is indoxoropyram and active ingredient B is triflumezopyrim, and the mass ratio of active ingredient A to active ingredient B is 50:1 to 1:

50.

2. The insecticidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 30:1 to 1:

20.

3. The insecticidal composition according to claim 2, characterized in that, The mass ratio of active ingredient A to active ingredient B is 16:1 to 1:

10.

4. The insecticidal composition according to claim 3, characterized in that, The mass ratio of active ingredient A to active ingredient B is 8:1 to 3:

2.

5. The insecticidal composition according to claim 1, characterized in that, The total weight of the insecticidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.01% to 80% of the total weight of the insecticidal composition.

6. The insecticidal composition according to claim 1, characterized in that, In addition to the active ingredient, the insecticidal composition contains pesticide-permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

7. The insecticidal composition according to claim 1, characterized in that, The insecticidal composition is prepared into a pesticide formulation that is permitted in pesticides, wherein the formulation is a solid or liquid formulation.

8. The insecticidal composition according to claim 7, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder; the liquid formulation is an emulsifiable concentrate, a suspension concentrate, a microemulsion, a dispersible oil suspension, or a water emulsion.

9. The application of the insecticidal composition according to any one of claims 1-8 for the control of agricultural, horticultural, or forestry pests, characterized in that, The pest in question is an aphid.

10. The application according to claim 9, characterized in that, Apply the insecticidal composition or formulation thereof according to any one of claims 1-8 to the pest that needs to be controlled or to the medium in which it grows.

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