An on-site raw water level collection environmental DNA sample device

Abstract

The application discloses a kind of field in-situ collection environmental DNA sample equipment, it is related to water body environmental DNA sample collection technical field, including collection box, filter box and connecting frame, the two sides of the collection box are installed with filter box, and the top end of filter box is installed with connecting frame, the front end of the filter box is provided with water inlet groove, and flow rate sensor is arranged in the inside of water inlet groove, the inside of the collection box and filter box is provided with horn mouth, the inside of the filter box is provided with conical filter cover for blocking impurities, the bottom end of the filter box is provided with position mounting mechanism for fixing the conical filter cover, the inside of the connecting frame is provided with reciprocating cleaning mechanism for cleaning the position of the conical filter cover, the device has collection filter membrane frame automatic liquid addition frame body to save, first motor drives another group of collection frame body to rotate to horn mouth position alignment, it is convenient to continue to collect water body DNA sample.

Description

Technical Field

[0001] This invention relates to the field of road traffic technology, specifically to a device for collecting environmental DNA samples at the original water level on-site. Background Technology

[0002] Environmental DNA, also known as EDNA, refers to the mixture of genomic DNA from all different organisms found in an environmental sample. The concept of an environmental sample for EDNA is very broad and can include soil, sediment, excrement, air, water, and even the organism itself (such as an insect captured by a mackerel web). Animals living in a particular environment leave traces of their DNA that are shed and dispersed in the surrounding area.

[0003] However, existing on-site environmental DNA sample collection equipment has the following problems during use: the fluidity of river water causes significant spatial and temporal variations in the aquatic environment; the randomness of the sampling time, frequency, and location may lead to samples that fail to reflect or represent the diversity of the research object in the river system, thus affecting the accuracy and completeness of sample analysis results. Therefore, current methods of collecting water samples and returning them to the laboratory for testing and analysis are not representative, and there is a need to develop an environmental DNA sample collection device that can collect and automatically preserve environmental DNA samples outdoors for extended periods to improve the accuracy of environmental DNA sample collection. Summary of the Invention

[0004] The purpose of this invention is to provide an on-site device for collecting environmental DNA samples at the original water level, thereby solving the problem of low sample accuracy obtained by existing collection methods.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an on-site device for collecting environmental DNA samples at the original water level, comprising a collection box, a filter box, and a connecting frame. Filter boxes are installed on both sides of the collection box, and a connecting frame is installed at the top of the filter box. A water inlet trough is provided at the front end of the filter box, and a flow rate sensor is installed inside the water inlet trough. A flared opening is provided inside the collection box and the filter box. A conical filter cover for blocking impurities is provided inside the filter box. A mounting mechanism for fixing the position of the conical filter cover is provided at the bottom end of the filter box. A reciprocating cleaning mechanism for cleaning the position of the conical filter cover is provided inside the connecting frame. An installation box is installed on the outer wall of the collection box, and a first motor is provided on one side of the installation box. A main shaft is installed at the output end of the first motor via a coupling, and a collection frame penetrating the flared opening is installed on the outer wall of the main shaft. A collection filter frame for collection is provided inside the collection frame, and a transmission mechanism for moving the position of the collection filter frame is provided inside the collection frame.

[0006] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The outer walls on both sides of the mounting box are hinged with sealing door panels, and a liquid collection frame is provided at the bottom of the mounting box.

[0007] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The installation mechanism includes a movable groove and a telescopic groove. The movable groove is provided inside the filter box, and a telescopic groove is provided on one side of the movable groove. A rotating shaft is installed inside the movable groove through a bearing, and a gear is installed on the outer wall of the rotating shaft. A rack that meshes with the gear is provided inside the telescopic groove, and one side of the rack penetrates one side of the conical filter cover. A spring is sleeved on the outer wall of the rack.

[0008] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The reciprocating cleaning mechanism includes a single-phase asynchronous motor and a splined shaft. The top of the connecting frame is equipped with a single-phase asynchronous motor, and the output end of the single-phase asynchronous motor is equipped with a lead screw via a coupling. The outer wall of the lead screw is fitted with a positioning nut, and the splined shaft is installed inside the positioning nut via a bearing. The inside of the connecting frame is equipped with a single-phase asynchronous motor via a bearing, and the inside of the single-phase asynchronous motor is connected to the splined shaft via positioning balls. The bottom end of the splined shaft is equipped with a cleaning frame for cleaning the inner wall of the conical filter cover.

[0009] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The connecting frame has a positioning block installed inside via bearings. The connecting frame also has a synchronous motor installed inside, and the output end of the synchronous motor is connected to a drive wheel via a coupling. The drive wheel and the outer wall of the positioning block are fitted with a conveyor belt for transmission.

[0010] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The transmission mechanism includes a fixed shaft and a transmission roller. A second motor is installed on the inner wall of the collection frame, and the output end of the second motor is connected to the fixed shaft via a coupling. A transmission roller that contacts the collection filter membrane frame is installed on the outer wall of the fixed shaft. The outer wall of the fixed shaft provides rotational power to another set of fixed shafts through a synchronous pulley and a transmission belt.

[0011] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level, wherein the inside of the flared mouth is equipped with a sealed airbag that fits tightly against the collection frame.

[0012] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level. The collection box is equipped with a limiting frame for fixing the position, and the limiting frame has a limiting shaft installed inside via bearings. The outer wall of the limiting shaft is fitted with a guide wheel, and the bottom end of the guide wheel is fitted with a pull rope connected to the top of the collection box. The outer wall of the limiting frame is threaded with a positioning screw that penetrates the outer wall of the limiting shaft. A third motor is provided on one side of the limiting frame, and the output end of the third motor is connected to one end of the limiting shaft via a coupling.

[0013] This technical solution provides an on-site device for collecting environmental DNA samples at the original water level, wherein a microcontroller and a timer are installed on the outer wall of the first motor.

[0014] Compared with existing technologies, the present invention provides an on-site device for collecting environmental DNA samples at the original water level, which has the following advantages:

[0015] 1. This invention features a flow rate sensor at the water inlet. When the flow rate is set to be lower than a predetermined value, a microcontroller activates a first motor to rotate the main shaft. This main shaft then drives the collection frame to compress the sealing airbag inside the funnel-shaped opening until the collection filter frame aligns with the funnel opening. The collection filter frame can then trap various environmental DNA samples in the water. A timer is set at the first motor. Upon reaching the set time, both the first and second motors are activated. The first motor rotates the collection frame until the collection filter frame aligns downwards with the liquid placement frame. The second motor then rotates the fixed shaft, which in turn drives the transmission roller to move the collection filter frame into the liquid placement frame. The collection filter frame is then automatically added to the liquid placement frame for storage. The first motor then rotates another set of collection frames to align with the funnel opening, facilitating further collection of environmental DNA samples. This makes collection convenient and operation simple.

[0016] 2. This invention uses a startable single-phase asynchronous motor to drive a lead screw to rotate, which in turn drives the spline shaft to move up and down via a positioning nut. Simultaneously, a synchronous motor drives a drive wheel to drive a positioning block to rotate via a conveyor belt. The ball bearings at the positioning block engage with the outer wall of the spline shaft, facilitating the rotation of the spline shaft. This structure can also synchronously drive the cleaning frame below the spline shaft to rotate and clean the conical filter cover, achieving a cleaning effect without the need for manual cleaning. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the front cross-sectional structure of the present invention;

[0018] Figure 2 This is a schematic diagram of the main structure of the present invention;

[0019] Figure 3This is a schematic diagram of the left sectional view of the present invention;

[0020] Figure 4 For the present invention Figure 1 A magnified structural diagram at point A;

[0021] Figure 5 For the present invention Figure 1 A magnified structural diagram at point B;

[0022] Figure 6 For the present invention Figure 1 A magnified structural diagram at point C;

[0023] Figure 7 For the present invention Figure 1 Enlarged structural diagram at point D;

[0024] Figure 8 This is a schematic diagram of the left-side structure of the present invention.

[0025] In the diagram: 1. Collection box; 2. Trumpet mouth; 3. Filter box; 4. Inlet tank; 5. Flow sensor; 6. Conical filter cover; 7. Connecting frame; 8. Mounting box; 9. First motor; 10. Main shaft; 11. Microcontroller; 12. Collection frame; 13. Limiting frame; 14. Limiting shaft; 15. Guide wheel; 16. Pull rope; 17. Single-phase asynchronous motor; 18. Positioning block; 19. Splined shaft; 20. Wire 21. Rod; 22. Conveyor belt; 23. Synchronous motor; 24. Cleaning rack; 25. Movable trough; 26. Rotating shaft; 27. Gear; 28. Telescopic trough; 29. ​​Rack; 30. Spring; 31. Sealing airbag; 32. Filter membrane collection rack; 33. Fixed shaft; 34. Drive roller; 35. Synchronous pulley; 36. Drive belt; 37. Liquid dispensing frame; 38. Second motor; 39. Third motor; 30. Positioning screw. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] Example 1, as Figure 1-5As shown, the present invention provides a technical solution: an on-site device for collecting environmental DNA samples at the original water level, comprising a collection box 1, a filter box 3, and a connecting frame 7. Filter boxes 3 are installed on both sides of the collection box 1, and the connecting frame 7 is installed on the top of the filter box 3. A water inlet trough 4 is provided at the front end of the filter box 3, and a flow rate sensor 5 is installed inside the water inlet trough 4. A flared opening 2 is provided inside the collection box 1 and the filter box 3. A conical filter cover 6 for blocking impurities is provided inside the filter box 3. A mounting mechanism for fixing the position of the conical filter cover 6 is provided at the bottom end of the filter box 3. The mounting mechanism includes a movable groove 24 and a telescopic groove 27. The movable groove 24 is provided inside the filter box 3, and a telescopic groove is provided on one side of the movable groove 24. 27. A rotating shaft 25 is installed inside the movable groove 24 via bearings, and a gear 26 is installed on the outer wall of the rotating shaft 25. A rack 28 that meshes with the gear 26 is provided inside the telescopic groove 27, and one side of the rack 28 passes through one side of the conical filter cover 6. A spring 29 is sleeved on the outer wall of the rack 28. When the operator installs the conical filter cover 6, the rotating shaft 25 can be rotated to drive the gear 26 to mesh with the rack 28, and the rack 28 will squeeze the spring 29. Then, the conical filter cover 6 can be inserted into the filter box 3 and fit into the flared mouth 2. At this time, the rotating shaft 25 is released, and the rack 28 is driven to insert into the bottom plate of the conical filter cover 6 under the elastic force of the spring 29. This structure facilitates the installation of the conical filter cover 6 and facilitates later replacement.

[0028] Example 2, as Figure 1-6As shown, the present invention provides a technical solution: an on-site device for collecting environmental DNA samples at the original water level, comprising a connecting frame 7 with a reciprocating cleaning mechanism for cleaning the conical filter hood 6. The reciprocating cleaning mechanism includes a single-phase asynchronous motor 17 and a splined shaft 19. The top of the connecting frame 7 is equipped with the single-phase asynchronous motor 17, and the output end of the single-phase asynchronous motor 17 is mounted with a lead screw 20 via a coupling. A positioning nut is sleeved on the outer wall of the lead screw 20, and the splined shaft 19 is mounted inside the positioning nut via a bearing. The single-phase asynchronous motor 17 is mounted inside the connecting frame 7 via a bearing, and the inside of the single-phase asynchronous motor 17 is connected to the splined shaft 19 via positioning balls. A cleaning frame 23 for cleaning the inner wall of the conical filter hood 6 is installed at the bottom of the splined shaft 19. The connecting frame 7 has a internal ventilation channel. A positioning block 18 is installed on the bearing. A synchronous motor 22 is installed inside the connecting frame 7. The output end of the synchronous motor 22 is connected to a drive wheel via a coupling. A conveyor belt 21 for transmission is fitted on the outer wall of the drive wheel and the positioning block 18. By starting the single-phase asynchronous motor 17, the lead screw 20 is driven to rotate. The lead screw 20 drives the spline shaft 19 to move up and down through the positioning nut. At the same time, the synchronous motor 22 drives the positioning block 18 to rotate through the drive wheel and the conveyor belt 21. The ball bearings at the positioning block 18 engage with the outer wall of the spline shaft 19, facilitating the rotation of the spline shaft 19. This structure can synchronously drive the cleaning frame 23 below the spline shaft 19 to rotate up and down to clean the conical filter cover 6, achieving a cleaning effect without manual cleaning, which is convenient for cleaning the sediment in the flowing river water.

[0029] Example 3, as Figure 1-7As shown, the present invention provides a technical solution: an on-site device for collecting environmental DNA samples at the original water level, comprising a collection box 1 with an installation box 8 mounted on its outer wall, a first motor 9 mounted on one side of the installation box 8, sealing door panels hinged to the outer walls on both sides of the installation box 8, a liquid discharge frame 36 at the bottom of the installation box 8, a main shaft 10 mounted on the output end of the first motor 9 via a coupling, and a collection frame 12 penetrating the interior of a funnel-shaped opening 2 mounted on the outer wall of the main shaft 10, with a collection filter membrane frame 31 for collection inside the collection frame 12. 2. An internal transmission mechanism for moving the collection filter membrane holder 31 is provided. The transmission mechanism includes a fixed shaft 32 and a transmission roller 33. A second motor 37 is provided on the inner wall of the collection frame 12, and the output end of the second motor 37 is connected to the fixed shaft 32 via a coupling. The transmission roller 33, which contacts the collection filter membrane holder 31, is installed on the outer wall of the fixed shaft 32. The outer wall of the fixed shaft 32 is equipped with a synchronous pulley 34 and a transmission belt 35 to provide rotational power to another set of fixed shafts 32. A sealing airbag 30 that fits tightly against the collection frame 12 is provided inside the flared opening 2. The outer wall of the machine 9 is equipped with a microcontroller 11 and a timer. A flow rate sensor 5 is located at the water inlet tank 4. When the flow rate is set to be lower than a set value, the microcontroller 11 activates the first motor 9, driving the main shaft 10 to rotate. The main shaft 10 then drives the collection frame 12 to squeeze the sealing airbag 30 inside the funnel mouth 2 until the collection filter membrane frame 31 is aligned with the funnel mouth 2. This allows the collection filter membrane frame 31 to trap various environmental DNA samples in the water. The first motor 9 is equipped with a timer; upon reaching a set time, the microcontroller 11 activates the second motor 9. A first motor 9 and a second motor 37 are used. The first motor 9 drives the collection frame 12 to rotate 90 degrees, so that the collection filter membrane frame 31 is aligned downward with the liquid placement frame 36. The second motor 37 is started to drive the fixed shaft 32 to rotate, so that the fixed shaft 32 drives the transmission roller 33 to move the collection filter membrane frame 31 down into the liquid placement frame 36. Then the collection filter membrane frame 31 is automatically added into the liquid placement frame 36 for preservation. Then the first motor 9 drives another set of collection frames 12 to rotate to the alignment position at the flared mouth 2, so as to continue collecting environmental DNA samples.

[0030] Example 4, as Figure 1-8As shown, the present invention provides a technical solution: an on-site device for collecting environmental DNA samples at the original water level, comprising a limiting frame 13 for fixing the position above the collection box 1, and a limiting shaft 14 installed inside the limiting frame 13 via bearings. A guide wheel 15 is sleeved on the outer wall of the limiting shaft 14, and a pull rope 16 connected to the top of the collection box 1 is installed at the bottom of the guide wheel 15. A positioning screw 39 is threaded through the outer wall of the limiting frame 13 and penetrates the outer wall of the limiting shaft 14. A third motor 38 is provided on one side of the limiting frame 13, and the output end of the third motor 38 is connected to one end of the limiting shaft 14 via a coupling. By removing the positioning screw 39, the third motor 38 is started to drive the limiting shaft 14 to rotate, thereby the limiting shaft 14 drives the guide wheel 15 to rotate, and then the guide wheel 15 drives the pull rope 16 to move downward, so as to lower the collection box 1 to a suitable position in the water.

[0031] Working principle: First, connect the external power supply. The operator can open the sealed door of the installation box 8 and add the extraction solution into the liquid placement frame 36. Then, place the collection filter frame 31 in the empty slot of the collection frame 12. Start the second motor 37 to drive the fixed shaft 32 to rotate. The fixed shaft 32 drives the transmission roller 33 to move the collection filter frame 31 into the collection frame 12 to a suitable position. After the installation of the collection filter frames 31 in the two collection frames 12 is completed, the limiting frame 13 is fixed on the bridge or road surface. Then, remove the positioning screw 39 and start the third motor 38 to drive the limiting shaft 14 to rotate. The limiting shaft 14 drives the guide wheel 15 to rotate. The guide wheel 15 then drives the pull rope 16 to move downward, making it easy to lower the collection box 1 to a suitable position in the water. Then, a large amount of sewage enters through the inlet tank 4, and impurities and sand in the water are filtered through the conical filter cover 6. Smaller environmental DNA samples are trapped in the collection filter frame 31. The flow rate sensor 5 can detect the flow rate of the sample. When the flow rate sensor 5 sets the flow rate to be lower than the set value, the microcontroller 11 starts the first motor 9 to drive the main shaft 10 to rotate. The main shaft 10 then drives the collection frame 12 to squeeze the sealing airbag 30 inside the funnel mouth 2 until the position of the collection filter frame 31 is aligned with the position of the funnel mouth 2. After that, the environmental DNA samples in the water can be intercepted through the collection filter frame 31. When a timer is set at the first motor 9, the first motor 9 and the second motor 37 can be started after the set time is reached. The first motor 9 drives the collection frame 12 to rotate 90 degrees, and the position of the collection filter frame 31 is aligned downward with the position of the liquid placement frame 36. The second motor 37 is started to drive the fixed shaft 32 to rotate. The fixed shaft 32 then drives the transmission roller 33 to move the collection filter frame 31 down into the liquid placement frame 36. The collection filter frame 31 is then automatically added to the liquid placement frame 36 for storage. Then, the first motor 9 drives another set of collection frames 12 to rotate to the aligned position of the funnel mouth 2 to facilitate the continued collection of environmental DNA samples.

[0032] When it is necessary to clean the impurities in the conical filter housing 6, the single-phase asynchronous motor 17 can be started to drive the lead screw 20 to rotate. The lead screw 20 then drives the spline shaft 19 to move up and down through the positioning nut. At the same time, the synchronous motor 22 is started to drive the positioning block 18 to rotate through the drive wheel and the conveyor belt 21. The ball bearings in the positioning block 18 and the outer wall of the spline shaft 19 engage with each other, which facilitates the rotation of the spline shaft 19. This structure can also drive the cleaning frame 23 below the spline shaft 19 to rotate up and down to clean the conical filter housing 6, achieving a cleaning effect without the need for manual cleaning.

[0033] When it is necessary to remove and replace the conical filter cover 6, the rotating shaft 25 can be rotated to drive the gear 26 to mesh with the rack 28. The rack 28 then compresses the spring 29. Next, the conical filter cover 6 can be inserted into the filter box 3 and fit into the flared mouth 2. At this time, the rotating shaft 25 is released, and the rack 28 is driven to insert into the bottom plate of the conical filter cover 6 under the elastic force of the spring 29. This structure facilitates the installation and removal of the conical filter cover 6 and facilitates its replacement later.

[0034] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A device for collecting environmental DNA samples at the original water level on-site, comprising a collection box (1), a filter box (3), and a connecting frame (7), characterized in that: Both sides of the collection box (1) are equipped with filter boxes (3), and the top of the filter box (3) is equipped with a connecting frame (7). The front end of the filter box (3) is provided with a water inlet trough (4), and a flow rate sensor (5) is provided inside the water inlet trough (4). The inside of the collection box (1) and the filter box (3) is provided with a flared opening (2). The inside of the filter box (3) is provided with a conical filter cover (6) for blocking impurities. The bottom end of the filter box (3) is provided with a mounting mechanism for fixing the position of the conical filter cover (6). The connecting frame (7) is provided with a... There is a reciprocating cleaning mechanism for cleaning the position of the conical filter cover (6). The outer wall of the collection box (1) is equipped with a mounting box (8), and a first motor (9) is provided on one side of the mounting box (8). The output end of the first motor (9) is equipped with a main shaft (10) through a coupling. The outer wall of the main shaft (10) is equipped with a collection frame (12) that penetrates the inside of the horn mouth (2). The collection frame (12) is equipped with a collection filter membrane frame (31) for collection. The collection frame (12) is equipped with a transmission mechanism for moving the position of the collection filter membrane frame (31).

2. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The outer walls on both sides of the mounting box (8) are hinged with sealing door panels, and the bottom of the mounting box (8) is provided with a liquid discharge frame (36).

3. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The installation mechanism includes a movable groove (24) and a telescopic groove (27). The movable groove (24) is provided inside the filter box (3), and a telescopic groove (27) is provided on one side of the movable groove (24). A rotating shaft (25) is installed inside the movable groove (24) through a bearing, and a gear (26) is installed on the outer wall of the rotating shaft (25). A rack (28) that meshes with the gear (26) is provided inside the telescopic groove (27), and one side of the rack (28) penetrates one side of the conical filter cover (6). A spring (29) is sleeved on the outer wall of the rack (28).

4. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The reciprocating cleaning mechanism includes a single-phase asynchronous motor (17) and a spline shaft (19). The top of the connecting frame (7) is provided with a single-phase asynchronous motor (17), and the output end of the single-phase asynchronous motor (17) is equipped with a lead screw (20) through a coupling. The outer wall of the lead screw (20) is fitted with a positioning nut, and the inside of the positioning nut is equipped with a spline shaft (19) through a bearing. The inside of the connecting frame (7) is equipped with a single-phase asynchronous motor (17) through a bearing, and the inside of the single-phase asynchronous motor (17) is connected to the spline shaft (19) through positioning balls. The bottom end of the spline shaft (19) is equipped with a cleaning frame (23) for cleaning the inner wall of the conical filter cover (6).

5. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The connecting frame (7) has a positioning block (18) installed inside by bearings. The connecting frame (7) has a synchronous motor (22) installed inside. The output end of the synchronous motor (22) is equipped with a drive wheel through a coupling. The drive wheel and the outer wall of the positioning block (18) are fitted with a conveyor belt (21) for transmission.

6. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The transmission mechanism includes a fixed shaft (32) and a transmission roller (33). The inner wall of the collection frame (12) is provided with a second motor (37), and the output end of the second motor (37) is connected to the fixed shaft (32) via a coupling. The outer wall of the fixed shaft (32) is connected to the transmission roller (33) which is in contact with the collection filter membrane frame (31). The outer wall of the fixed shaft (32) is connected to the other fixed shaft (32) via a synchronous pulley (34) and a transmission belt (35).

7. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The flared opening (2) is equipped with a sealed airbag (30) that fits tightly against the collection frame (12).

8. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The collection box (1) is provided with a limiting frame (13) for fixing the position, and the limiting frame (13) is equipped with a limiting shaft (14) through a bearing. The outer wall of the limiting shaft (14) is fitted with a guide wheel (15), and the bottom end of the guide wheel (15) is equipped with a pull rope (16) connected to the top of the collection box (1). The outer wall of the limiting frame (13) is provided with a positioning screw (39) that penetrates the outer wall of the limiting shaft (14) through a thread. A third motor (38) is provided on one side of the limiting frame (13), and the output end of the third motor (38) is connected to one end of the limiting shaft (14) through a coupling.

9. The device for collecting environmental DNA samples at the original water level in situ according to claim 1, characterized in that: The outer wall of the first motor (9) is equipped with a microcontroller (11) and a timer.

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