Method for preparing luminol and copper ion bifunctionalized magnetic core-shell structure nanoparticles

By modifying the Fe3O4@Au core-shell structure surface with luminol and cysteine ​​to immobilize copper ions and constructing a catalytic center for the luminol-hydrogen peroxide reaction, the problems of easy aggregation and insufficient chemical stability of magnetic nanoparticles are solved, and ultrasensitive detection of pyrophosphate ions is achieved, which is suitable for bioanalysis and food safety monitoring.

CN122213982APending Publication Date: 2026-06-16WUHAN TEXTILE UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-24
Publication Date
2026-06-16

AI Technical Summary

Technical Problem

Existing magnetic nanoparticles tend to aggregate, have limited surface activity, and lack sufficient chemical stability when detecting pyrophosphate ions. Traditional chemiluminescence systems also suffer from insufficient signal stability and quantitative reliability in complex environments.

Method used

By modifying the Fe3O4@Au core-shell structure surface with luminol and cysteine, copper ions are immobilized to form the catalytic center for the luminol-hydrogen peroxide reaction. The signal on-off mechanism is realized by the competitive binding of copper ions and pyrophosphate, thus constructing a highly efficient chemiluminescence detection platform.

Benefits of technology

It achieves ultrasensitive detection of pyrophosphate ions with a detection limit of 8.37 pM, exhibiting a wide detection range and good signal stability, making it suitable for bioanalysis and food safety monitoring.

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Abstract

The application discloses a preparation method of a luminol and copper ion bifunctional magnetic core-shell structure nanoparticle, and is used for super-sensitive detection of pyrophosphate ions. The nanoparticle contains a Fe3O4@Au core-shell structure, the core-shell structure is generated by in-situ reduction of Au on a Fe3O4 magnetic core surface by using luminol 3+ A gold shell is generated, and cysteine is further modified on the surface of the core-shell structure, which provides specific coordination sites for copper ions, the copper ions are captured and fixed on the surface of the gold shell through the sites, the copper ions play a catalytic role in a luminol-hydrogen peroxide system, a stable'signal opening' state is formed, when pyrophosphate is introduced into the system, the copper ions can be competitively combined and the surface catalytic center can be weakened, so that the luminous intensity is reduced, the method has a good linear response relationship in the range of 0.1 nM-10 muM, and the detection limit reaches 8.37 pM.
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