A method and system for evaluating the effectiveness of an immunization intervention

Abstract

The application is suitable for the technical field of intelligent evaluation, and provides an effect evaluation method and system suitable for immune intervention measures, which comprises the following steps: firstly, accurately obtaining first TCR repertoire data information and second TCR repertoire data information of a target object; then, determining first immune age information according to the first TCR repertoire data information, and determining second immune age information according to the second TCR repertoire data information; and finally, effectively generating evaluation result information based on the above. The application can establish a brand-new and highly sensitive end-point index, which can be used for the evaluation of clinical trials and health products. The index quantifies the specific influence of intervention measures on the 'youthfulness' of the immune system, provides objective and automated judgment criteria, and evaluates the effectiveness of immune intervention measures, thereby improving the reliability of the evaluation, providing a more solid foundation for scientific research and clinical application, promoting the research and optimization of health products, and better meeting individual health needs.

Description

Technical Field

[0001] This application relates to the technical field of intelligent assessment, and more specifically, to a method and system for evaluating the effectiveness of immune interventions. Background Technology

[0002] Detecting an individual's immune age has become an important area of ​​biomedical research in recent years. Its main purpose is to assess an individual's immune system function and health status. Immune age may differ from chronological age, reflecting the degree of aging and health of an individual's immune system.

[0003] Currently, the effectiveness of immune intervention measures usually depends on the subjective judgment of professional doctors. Due to the high degree of subjectivity, it may lead to misdiagnosis of individual health conditions, resulting in low reliability, which needs further improvement. Summary of the Invention

[0004] Based on this, embodiments of this application provide a method and system for evaluating the effectiveness of immune intervention measures, in order to solve the problem of low reliability in the prior art.

[0005] In a first aspect, embodiments of this application provide a method for evaluating the effectiveness of immune intervention measures, the method comprising: Obtain first TCR library data information and second TCR library data information of the target object, wherein the first TCR library data information is used to describe the TCR library data information of the target object before the immune intervention, and the second TCR library data information is used to describe the TCR library data information of the target object after the immune intervention. Based on the first TCR library data, the first immune age information is determined, and based on the second TCR library data, the second immune age information is determined. Evaluation results are generated based on the first and second immune age information.

[0006] Compared with the prior art, the beneficial effects are as follows: The method for evaluating the effectiveness of immune intervention measures provided in this application embodiment can first accurately obtain the first TCR repertoire data information and the second TCR repertoire data information of the target object, then quickly determine the first immune age information based on the first TCR repertoire data information, and simultaneously quickly determine the second immune age information based on the second TCR repertoire data information. Finally, the evaluation result information is effectively generated based on the first immune age information and the second immune age information, thereby realizing automatic and objective judgment on whether the immune intervention measures are effective, effectively improving reliability, and solving the problem of low reliability in the current system to a certain extent.

[0007] Secondly, embodiments of this application provide an efficacy evaluation system suitable for immune intervention measures, the system comprising: TCR library data acquisition module: used to acquire the first TCR library data information and the second TCR library data information of the target object, wherein the first TCR library data information is used to describe the TCR library data information before the immune intervention of the target object, and the second TCR library data information is used to describe the TCR library data information after the immune intervention of the target object. Immune age information determination module: used to determine first immune age information based on the first TCR library data information, and to determine second immune age information based on the second TCR library data information; Evaluation result information generation module: used to generate evaluation result information based on the first immune age information and the second immune age information.

[0008] Thirdly, embodiments of this application provide a terminal device, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the steps of the method described in the first aspect above.

[0009] Fourthly, embodiments of this application provide a computer-readable storage medium storing a computer program that, when executed by a processor, implements the steps of the method described in the first aspect above.

[0010] It is understood that the beneficial effects of the second to fourth aspects mentioned above can be found in the relevant descriptions in the first aspect mentioned above, and will not be repeated here. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0012] Figure 1 This is a flowchart illustrating an embodiment of the effect evaluation method provided in this application; Figure 2 This is a flowchart illustrating step S300 in an effect evaluation method provided in an embodiment of this application; Figure 3 This is a flowchart illustrating the process after step S300 in an embodiment of the effect evaluation method provided in this application; Figure 4 This is a flowchart illustrating the process after step S420 in an embodiment of the effect evaluation method provided in this application; Figure 5This is a flowchart illustrating the process after step S422 in an embodiment of the effect evaluation method provided in this application; Figure 6 This is a block diagram of an effect evaluation system provided in an embodiment of this application; Figure 7 This is a schematic diagram of a terminal device provided in an embodiment of this application. Detailed Implementation

[0013] In the following description, specific details such as particular system architectures and techniques are set forth for illustrative purposes and not for limitation, in order to provide a thorough understanding of the embodiments of this application. However, those skilled in the art will understand that this application may also be implemented in other embodiments without these specific details. In other instances, detailed descriptions of well-known systems, apparatuses, circuits, and methods have been omitted so as not to obscure the description of this application with unnecessary detail.

[0014] In the description of this application and the appended claims, the terms "first," "second," "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0015] References to "one embodiment" or "some embodiments" as described in this specification mean that one or more embodiments of this application include a specific feature, structure, or characteristic described in connection with that embodiment. Therefore, the phrases "in one embodiment," "in some embodiments," "in other embodiments," "in still other embodiments," etc., appearing in different parts of this specification do not necessarily refer to the same embodiment, but rather mean "one or more, but not all, embodiments," unless otherwise specifically emphasized. The terms "comprising," "including," "having," and variations thereof mean "including but not limited to," unless otherwise specifically emphasized.

[0016] To illustrate the technical solution described in this application, specific embodiments are provided below.

[0017] Please see Figure 1 , Figure 1 This is a flowchart illustrating the method for evaluating the effectiveness of immune intervention measures provided in this application embodiment. In this embodiment, the execution subject of the effectiveness evaluation method is a terminal device. It is understood that the types of terminal devices include, but are not limited to, mobile phones, tablets, laptops, Ultra-Mobile Personal Computers (UMPCs), netbooks, Personal Digital Assistants (PDAs), etc. This application embodiment does not impose any restrictions on the specific type of terminal device.

[0018] Please see Figure 1 The effect evaluation method provided in this application includes, but is not limited to, the following steps: In S100, the first TCR group library data information and the second TCR group library data information of the target object are obtained.

[0019] Specifically, the terminal device can first obtain the first TCR repertoire data information and the second TCR repertoire data information of the target object. The first TCR repertoire data information is used to describe the TCR repertoire data information before the immune intervention measures are taken on the target object, and the second TCR repertoire data information is used to describe the TCR repertoire data information after the immune intervention measures are taken on the target object.

[0020] In S200, the first immune age information is determined based on the first TCR repertoire data information, and the second immune age information is determined based on the second TCR repertoire data information.

[0021] Specifically, after the terminal device acquires the first TCR repertoire data information and the second TCR repertoire data information, the terminal device can quickly determine the first immune age information based on the first TCR repertoire data information, and simultaneously quickly determine the second immune age information based on the second TCR repertoire data information. For example, the terminal device can determine the immune age through the TCR repertoire data information using existing technologies, which will not be elaborated here. For instance, the CDR3 sequence of the TCR can be obtained from the blood sample of the target object, and its diversity index (such as Shannon entropy), clonality (such as the maximum clonal ratio), and the bioinformatics characteristics of the sequence itself (such as the CDR3 length distribution and the frequency of V / J gene use) can be calculated. Then, these features are used as inputs, and a machine learning model (such as a random forest model) that has been pre-trained on a cohort of people with known ages and health conditions is used for fitting and prediction, and finally an estimated "immune age" representing the functional state of an individual's immune system is output.

[0022] In S300, evaluation result information is generated based on the first and second immunization age information.

[0023] Specifically, after the terminal device determines the first and second immunization age information, it can effectively generate evaluation result information based on this information. This evaluation result information includes information on ineffective intervention, effective delay, or effective reversal. Ineffective intervention information describes immune interventions as ineffective, effective delay information describes immune interventions as effectively delaying immune aging, and effective reversal information describes immune interventions as effectively reversing immune aging.

[0024] For some possible implementations, please refer to [link to relevant documentation] for information on effectively generating evaluation results. Figure 2 Step S300 includes, but is not limited to, the following steps: In S310, an immune age difference is generated based on the first immune age information and the second immune age information.

[0025] Specifically, after the terminal device determines the first immune age information and the second immune age information, the terminal device can effectively generate an immune age difference value based on the first immune age information and the second immune age information. The immune age difference value is used to describe the difference between the first immune age information and the second immune age information.

[0026] In S320, if the difference in immune age is positive, the evaluation result information is determined to be invalid intervention information.

[0027] Specifically, if the difference in immune age is positive, it indicates that the immune age before the immunization intervention is still less than the immune age after the immunization intervention. Therefore, the terminal device can determine that the evaluation result information is invalid intervention information.

[0028] In S330, if the immune age difference is negative and the immune age difference is greater than the preset immune age threshold, then the evaluation result is determined to be effective delay information.

[0029] Specifically, if the immune age difference is negative and greater than the preset immune age threshold, it indicates that the immune age before the immune intervention is greater than the immune age after the intervention, indicating a good effect in delaying immune aging. Therefore, the terminal device can determine that the evaluation result is an effective delaying result.

[0030] In S340, if the immune age difference is negative and the immune age difference is less than or equal to the immune age threshold value, then the evaluation result information is determined to be effective reversal information.

[0031] Specifically, if the immune age difference is negative and is less than or equal to the immune age threshold, it indicates that the immune age before the immune intervention is greater than the immune age after the intervention, which has an excellent effect on delaying immune aging. Therefore, the terminal device can determine that the evaluation result is effective reversal information.

[0032] For information on possible implementations and to help determine effective immunization interventions, please refer to [link / reference needed]. Figure 3 If the evaluation result is valid reversal information, then after step S300, the method further includes, but is not limited to, the following steps: In S400, intervention combination information is obtained to acquire effective reversal information.

[0033] Specifically, the terminal device can obtain information on intervention combinations that effectively reverse the situation. These intervention combinations may include taking probiotics, specific medications, and / or lifestyle interventions.

[0034] In S410, based on the intervention combination information, the evaluation result information corresponding to multiple other objects is obtained.

[0035] Specifically, after the terminal device obtains the intervention combination information, it can obtain evaluation result information for multiple other objects based on the intervention combination information. The other objects are used to describe other personnel who have received immunization intervention measures besides the target object.

[0036] In S420, if the evaluation results for multiple other objects are all effective reversal information, then the intervention combination information is determined to be the recommended combination information.

[0037] Specifically, if the evaluation results for multiple other subjects are all effective reversal information, it indicates that the immune intervention has an excellent effect on delaying immune aging for multiple non-specific individuals. Therefore, the terminal device can determine that the intervention combination information corresponding to the effective reversal information is the recommended combination information.

[0038] For information on possible implementations and to help determine the optimal immune intervention, please refer to [link / reference needed]. Figure 4 Prior to step S420, the method also includes, but is not limited to, the following steps: In S421, based on multiple effective reversal information, the smallest difference in immune age is determined as the target difference information.

[0039] Specifically, after the terminal device determines the recommended combination information, the terminal device can determine the smallest difference in immune age among multiple effective reversal information as the target difference information.

[0040] In S422, the intervention combination information corresponding to the target difference information is determined as the current optimal combination information.

[0041] Specifically, after the terminal device determines the target difference information, it can determine the intervention combination information corresponding to the target difference information as the current optimal combination information, thereby determining the optimal immune intervention measures. In some possible implementations, to facilitate the analysis of immunization results using big data technologies, please refer to [link / reference]. Figure 5 After step S422, the method further includes, but is not limited to, the following steps: In S423, the first number of test participants and the first intervention duration information corresponding to the recommended combination information are obtained, as well as the second number of test participants and the second intervention duration information corresponding to the current optimal combination information.

[0042] Specifically, after the terminal device determines the current optimal combination information, the terminal device can obtain the first number of test participants and the first intervention duration information corresponding to the recommended combination information, as well as the second number of test participants and the second intervention duration information corresponding to the current optimal combination information. The first number of test participants information describes the cumulative number of test participants for the recommended combination information; the first intervention duration information describes the average execution time of the recommended combination information for personnel performing immunization interventions; the second number of test participants information describes the cumulative number of test participants for the current optimal combination information; and the second intervention duration information describes the average execution time of the current optimal combination information for personnel performing immunization interventions.

[0043] In S424, a first associated data group is generated based on the recommended combination information, the first number of test participants information, and the first intervention duration information, and a second associated data group is generated based on the current optimal combination information, the second number of test participants information, and the second intervention duration information.

[0044] Specifically, after the terminal device obtains the first number of test participants, the first intervention duration, the second number of test participants, and the second intervention duration, the terminal device can generate a first associated data group based on the recommended combination information, the first number of test participants, and the first intervention duration, and generate a second associated data group based on the current optimal combination information, the second number of test participants, and the second intervention duration. The first associated data group information is used to describe the data combination consisting of recommended combination information, first number of test participants information, and first intervention duration information. The second associated data group information is used to describe the data combination consisting of current optimal combination information, second number of test participants information, and second intervention duration information.

[0045] In S425, the information of the first associated data group and the information of the second associated data group are uploaded to the test database.

[0046] Specifically, after the terminal device generates the first associated data group information and the second associated data group information, the terminal device can upload the first associated data group information and the second associated data group information to the test database.

[0047] The implementation principle of the method for evaluating the effectiveness of immune intervention measures in this application embodiment is as follows: The terminal device can first accurately obtain the first TCR repertoire data information and the second TCR repertoire data information of the target object, and then quickly determine the first immune age information based on the first TCR repertoire data information. At the same time, it can quickly determine the second immune age information based on the second TCR repertoire data information. Finally, it can effectively generate evaluation result information based on the first immune age information and the second immune age information, thereby realizing automatic and objective judgment on whether the immune intervention measures are effective and effectively improving reliability.

[0048] It should be noted that the sequence number of each step in the above embodiments does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0049] Embodiments of this application also provide an efficacy evaluation system suitable for immune intervention measures. For ease of explanation, only the parts relevant to this application are shown, such as... Figure 6 As shown, the system 60 includes: TCR library data acquisition module 61: used to acquire the first TCR library data information and the second TCR library data information of the target object, wherein the first TCR library data information is used to describe the TCR library data information before the immune intervention of the target object, and the second TCR library data information is used to describe the TCR library data information after the immune intervention of the target object. Immune age information determination module 62: used to determine first immune age information based on first TCR library data information, and to determine second immune age information based on second TCR library data information; Evaluation result information generation module 63: used to generate evaluation result information based on the first immunization age information and the second immunization age information.

[0050] Optionally, the evaluation result information includes information on ineffective intervention, effective delay, or effective reversal; the evaluation result information generation module 63 includes: The immune age difference generation submodule is used to generate an immune age difference based on the first immune age information and the second immune age information. The immune age difference is used to describe the difference between the first immune age information and the second immune age information. Ineffective intervention information determination submodule: If the difference in immune age is positive, the evaluation result information is determined to be ineffective intervention information; The effective delay information determination submodule is used to determine the evaluation result information as effective delay information if the immune age difference value is negative and the immune age difference value is greater than the preset immune age limit value. The effective reversal information determination submodule is used to determine the evaluation result information as effective reversal information if the immune age difference is negative and the immune age difference is less than or equal to the immune age threshold value.

[0051] Optionally, if the evaluation result information is valid reversal information, then the system 60 further includes: Intervention combination information acquisition module: Used to acquire intervention combination information that effectively reverses the disease; Evaluation Result Information Acquisition Module: Used to acquire evaluation result information for multiple other objects based on intervention combination information; Recommended combination information determination module: If the evaluation results of multiple other objects are all effective reversal information, then the intervention combination information is determined as the recommended combination information.

[0052] Optionally, the system 60 also includes: Target difference information determination module: used to determine the minimum immune age difference as the target difference information based on multiple effective reversal information; Current Optimal Combination Information Determination Module: Used to determine the intervention combination information corresponding to the target difference information as the current optimal combination information.

[0053] Optionally, the system 60 also includes: Test participant information acquisition module: Used to acquire the first test participant information and the first intervention duration information corresponding to the recommended combination information, as well as the second test participant information and the second intervention duration information corresponding to the current optimal combination information; The associated data group information generation module is used to generate first associated data group information based on recommended combination information, first test participant information, and first intervention duration information, and to generate second associated data group information based on current optimal combination information, second test participant information, and second intervention duration information. Associated Data Group Information Upload Module: This module is used to upload the information of the first and second associated data groups to the test database.

[0054] It should be noted that the information interaction and execution process between the above modules are based on the same concept as the method embodiments of this application. For details on their specific functions and technical effects, please refer to the method embodiments section, which will not be repeated here.

[0055] This application also provides a terminal device, such as... Figure 7As shown, the terminal device 70 of this embodiment includes: a processor 71, a memory 72, and a computer program 73 stored in the memory 72 and executable on the processor 71. When the processor 71 executes the computer program 73, it implements the steps in the above-described effect evaluation method embodiment, for example... Figure 1 Steps S100 to S300 are shown; or, when processor 71 executes computer program 73, it implements the functions of each module in the above-described device, for example... Figure 6 The functions of modules 61 to 63 are shown.

[0056] The terminal device 70 can be a desktop computer, laptop, handheld computer, cloud server, or other computing device, and includes, but is not limited to, a processor 71 and a memory 72. Those skilled in the art will understand that... Figure 7 This is merely an example of terminal device 70 and does not constitute a limitation on terminal device 70. It may include more or fewer components than shown, or combine certain components, or different components. For example, terminal device 70 may also include input / output devices, network access devices, buses, etc.

[0057] The processor 71 can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc.; the general-purpose processor can be a microprocessor or any conventional processor, etc.

[0058] The memory 72 can be an internal storage unit of the terminal device 70, such as the hard disk or memory of the terminal device 70. The memory 72 can also be an external storage device of the terminal device 70, such as a plug-in hard disk, smart media card (SMC), secure digital (SD) card, flash card, etc., equipped on the terminal device 70. Furthermore, the memory 72 can include both internal storage units and external storage devices of the terminal device 70. The memory 72 can also store computer program 73 and other programs and data required by the terminal device 70. The memory 72 can also be used to temporarily store data that has been output or will be output.

[0059] One embodiment of this application also provides a computer-readable storage medium storing a computer program that, when executed by a processor, can implement the steps of the various method embodiments described above. The computer program includes computer program code, which can be in the form of source code, object code, executable file, or some intermediate form. The computer-readable medium can include any entity or device capable of carrying computer program code, a recording medium, a USB flash drive, a portable hard drive, a magnetic disk, an optical disk, a computer memory, a read-only memory (ROM), a random access memory (RAM), an electrical carrier signal, a telecommunication signal, and a software distribution medium, etc.

[0060] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the methods, principles and structures of this application should be covered within the scope of protection of this application.

Claims

1. A method for evaluating the effectiveness of immune intervention measures, characterized in that, The method includes: Obtain first TCR library data information and second TCR library data information of the target object, wherein the first TCR library data information is used to describe the TCR library data information of the target object before the immune intervention, and the second TCR library data information is used to describe the TCR library data information of the target object after the immune intervention. Based on the first TCR library data, the first immune age information is determined, and based on the second TCR library data, the second immune age information is determined. Evaluation results are generated based on the first and second immune age information.

2. The method according to claim 1, characterized in that, The evaluation results include information on ineffective intervention, effective delay, or effective reversal. The step of generating evaluation result information based on the first and second immune age information includes: An immune age difference is generated based on the first immune age information and the second immune age information, wherein the immune age difference is used to describe the difference between the first immune age information and the second immune age information. If the immune age difference is positive, then the evaluation result information is determined to be invalid intervention information; If the immune age difference is negative and the immune age difference is greater than the preset immune age threshold, then the evaluation result is determined to be effective delay information. If the immune age difference is negative, and the immune age difference is less than or equal to the immune age threshold value, then the evaluation result information is determined to be effective reversal information.

3. The method according to claim 2, characterized in that, If the evaluation result information is valid reversal information, then after generating the evaluation result information based on the first immune age information and the second immune age information, the method further includes: Intervention combination information for obtaining the effective reversal information; Based on the intervention combination information, obtain evaluation result information for multiple other objects; If the evaluation results for multiple other objects are all effective reversal information, then the intervention combination information is determined to be the recommended combination information.

4. The method according to claim 3, characterized in that, After determining that the intervention combination information is a recommended combination information if the evaluation result information corresponding to multiple other objects are all effective reversal information, the method further includes: Based on multiple effective reversal information, the smallest difference in immune age is determined as the target difference information; The intervention combination information corresponding to the target difference information is determined as the current optimal combination information.

5. The method according to claim 4, characterized in that, After determining that the intervention combination information corresponding to the target difference information is the current optimal combination information, the method further includes: Obtain the first number of test participants and the first intervention duration information corresponding to the recommended combination information, as well as the second number of test participants and the second intervention duration information corresponding to the current optimal combination information; Based on the recommended combination information, the first number of test participants information, and the first intervention measure execution duration information, a first associated data group information is generated, and based on the current optimal combination information, the second number of test participants information, and the second intervention measure execution duration information, a second associated data group information is generated. Upload the information of the first associated data group and the information of the second associated data group to the test database.

6. A system for evaluating the effectiveness of immune interventions, characterized in that, The system includes: TCR library data acquisition module: used to acquire the first TCR library data information and the second TCR library data information of the target object, wherein the first TCR library data information is used to describe the TCR library data information before the immune intervention of the target object, and the second TCR library data information is used to describe the TCR library data information after the immune intervention of the target object. Immune age information determination module: used to determine first immune age information based on the first TCR library data information, and to determine second immune age information based on the second TCR library data information; Evaluation result information generation module: used to generate evaluation result information based on the first immune age information and the second immune age information.

7. The system according to claim 6, characterized in that, The evaluation results include information on ineffective intervention, effective delay, or effective reversal. The evaluation result information generation module includes: Immune age difference generation submodule: used to generate an immune age difference based on the first immune age information and the second immune age information, wherein the immune age difference is used to describe the difference between the first immune age information and the second immune age information. Ineffective intervention information determination submodule: used to determine that the evaluation result information is ineffective intervention information if the immune age difference value is positive; Effective delay information determination submodule: if the immune age difference value is negative and the immune age difference value is greater than the preset immune age limit value, then determine the evaluation result information as effective delay information; The effective reversal information determination submodule is used to determine the evaluation result information as effective reversal information if the immune age difference is negative and the immune age difference is less than or equal to the immune age limit value.

8. A terminal device, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, characterized in that, When the processor executes the computer program, it implements the steps of the method as described in any one of claims 1 to 5.

9. A computer-readable storage medium storing a computer program, characterized in that, When the computer program is executed by a processor, it implements the steps of the method as described in any one of claims 1 to 5.

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