A synergistic adjuvant composition for herbicides and its preparation method and application

Abstract

The present application belongs to the field of pesticide adjuvant and green agricultural technology, and particularly relates to a synergistic adjuvant composition for herbicides, and a preparation method and application thereof.The synergistic adjuvant composition for herbicides comprises the following components in percentage by weight: 10-35% of complex enzyme glycoside, 2-8% of rhamnolipid, 0.1-0.5% of defoaming agent, and 56.5-86.8% of deionized water.The complex enzyme glycoside and the rhamnolipid are compounded for the first time in the field of broad-spectrum herbicide reduction and synergism, and through the synergistic effect of the two, the absorption and conduction of the herbicide can be promoted, the application effect of the herbicide can be significantly improved, the amount of the herbicide can be reduced by 30-50%, the risk of crop phytotoxicity can be reduced, the effects of stress resistance, growth promotion, quality improvement and yield increase are achieved, and the composition is fully biodegradable and has no environmental residues.

Description

Technical Field

[0001] This invention belongs to the field of pesticide adjuvants and green agriculture technology, specifically relating to a herbicide synergist composition, its preparation method and application. Background Technology

[0002] Chemical herbicides are currently the core means of weed control in farmland, characterized by high efficiency, time-saving, and labor-saving features, playing an irreplaceable role in ensuring stable and increased yields of food and cash crops. Therefore, developing efficient, green, and broad-spectrum herbicide adjuvants has become a research hotspot and core demand in the agricultural field.

[0003] Herbicide synergists can be classified into surfactant synergists, oil synergists, inorganic salt synergists, and polymer synergists according to their chemical structure and source. Existing adjuvants all have significant technical defects: While organosilicon surfactants can quickly reduce the surface tension of pesticide solutions, they are easily hydrolyzed, have poor storage stability, and their synergistic effect decreases significantly in hard water and low-temperature environments. Some varieties also pose a risk of crop damage. Mineral oil and vegetable oil adjuvants can increase the amount of pesticide solution retained on the leaf surface, but they are greatly affected by environmental temperature, rainfall, and weed species, resulting in poor synergistic stability. Furthermore, mineral oils are difficult to biodegrade, and long-term use can easily cause soil pollution. Nonionic and anionic surfactants have poor versatility, and their synergistic effect on herbicides with different mechanisms of action varies significantly, failing to achieve broad-spectrum synergistic effects. Most petrochemical-based products have insufficient biodegradability. Bio-based adjuvants, represented by GigaDevice and Anron, have solved the environmental friendliness problem, but their synergistic effect on herbicides is limited, typically only achieving a 20% to 30% reduction in herbicide dosage. They also lack crop stress resistance and growth-promoting functions, failing to simultaneously achieve "efficient weed control" and "seedling protection and yield increase," thus failing to meet the diversified needs of modern agriculture.

[0004] In recent years, research has focused on incorporating biostimulants into herbicide formulations to enhance herbicide efficacy and alleviate phytotoxicity. Among existing technologies, Chinese invention patent CN121312620A discloses a herbicidal composition containing benzoxazine or its salts. By adding complex enzyme glycosides as biostimulants to benzoxazine formulations, it can alleviate phytotoxicity to corn under high-temperature conditions while slightly improving its efficacy. However, this technical solution has insurmountable core limitations: First, it only targets the single herbicide benzoxazine, without verifying its synergistic effect on other herbicides, lacking broad-spectrum compatibility and failing to meet the needs of reducing herbicide dosage and enhancing efficacy in field production; second, it can only alleviate high-temperature phytotoxicity of specific herbicides, without a clear effect on significant herbicide reduction, and also lacks broad-spectrum crop stress resistance, growth promotion, quality improvement, and yield enhancement effects, resulting in a single function.

[0005] Complex enzyme glycosides are a class of bioproducts rich in glycoside active substances. In the current technology, there are no reports on their application in enhancing the efficacy of broad-spectrum herbicides, and no related technologies have revealed their universal enhancement mechanism for different herbicides, or the technical effect of achieving a significant reduction in herbicide dosage. Summary of the Invention

[0006] This invention addresses the shortcomings of existing technologies by providing a herbicide synergist composition, its preparation method, and its application. This invention is the first to combine complex enzyme glycosides and rhamnolipids in the field of broad-spectrum herbicide reduction and enhancement. Through their synergistic effect, herbicide absorption and translocation are promoted, significantly improving herbicide application efficacy and achieving a 30%–50% reduction in herbicide dosage. Simultaneously, it reduces crop phytotoxicity risks, while also providing stress resistance, growth promotion, quality improvement, and yield enhancement. It is fully biodegradable with no environmental residue. This invention overcomes the limitations of existing herbicide synergists, such as limited synergistic effect, poor versatility, insufficient environmental friendliness, and lack of crop protection functions. It also solves the technical pain points of existing complex enzyme glycosides, which can only be applied to a single herbicide variety, have limited application scenarios, and limited functions.

[0007] The specific technical solution is as follows: The first objective of this invention is to provide a herbicide synergist composition comprising, by weight percentage: 10% to 35% complex enzyme glycosides, 2% to 8% rhamnolipids, 0.1% to 0.5% defoamer, and 56.5% to 86.8% deionized water.

[0008] The synergistic adjuvant composition is a standalone adjuvant formulation that does not contain the active ingredient of a herbicide.

[0009] This invention is the first to discover that the combination of complex enzyme glycosides and rhamnolipids exhibits excellent broad-spectrum synergistic effects on various herbicides. Through formulation optimization, a herbicide synergist composition with complex enzyme glycosides and rhamnolipids as its core components was prepared. This composition not only significantly reduces herbicide dosage and substantially improves control efficacy, but also broadly reduces phytotoxicity from various herbicides, promotes crop growth, and enhances stress resistance. Furthermore, it is fully biodegradable with no environmental residue, filling a gap in existing technologies and possessing significant practical significance and application value. The synergist composition of this invention is suitable for broad-spectrum herbicide reduction and efficacy enhancement in weed control of various field crops, cash crops, and fruit and vegetable crops, and is particularly suitable for improving efficacy and reducing dosage in herbicide field application scenarios.

[0010] Furthermore, the complex enzyme glycoside is a microbial directional fermentation product independently developed by Yantai Goodlife Biotechnology Co., Ltd.

[0011] Furthermore, the rhamnolipid was purchased from Shanghai Maclean Biochemical Technology Co., Ltd., and the content of the active ingredient is ≥98%.

[0012] Furthermore, the defoamer is an organosilicon defoamer, purchased from Sinopharm Chemical Reagent Co., Ltd.

[0013] The above-mentioned technical solution of the present invention has the following technical effects: 1. This study is the first to develop the application of complex enzyme glycosides and rhamnolipids in the field of broad-spectrum herbicide synergistic effects, clarifying the synergistic effect mechanism and standardized application methods of the two, and filling the existing technological gap. 2. A herbicide synergist composition with complex enzyme glycosides and rhamnolipids as the core was prepared. It completely removed traditional petrochemical-based wetting and dispersing agents. The formula is green and environmentally friendly, with excellent stability. It is suitable for various field application scenarios such as manual backpack spraying and drone spraying. It is an independent adjuvant formulation without herbicide active ingredients and can be flexibly adapted to various herbicide varieties. 3. Through the synergistic effect of complex enzyme glycosides and rhamnolipids, a broad-spectrum synergistic effect is achieved on herbicides. It has a significant synergistic effect on a variety of herbicides with different mechanisms of action, such as sulfonylureas, triketones, organophosphates, pyridine carboxylic acids, aryloxyphenoxypropionic acids, and diphenyl ethers (excluding benzoxazine and its salts). The combined effect of the two is far superior to the use of single components alone. It can reduce the amount of herbicide used by 30% to 50%, and the control effect is still significantly better than the conventional amount of single herbicide used. This solves the problem that the existing technology can only be used with a single herbicide. 4. It also has broad-spectrum crop protection functions, which can reduce the risk of phytotoxicity to target crops by various herbicides, while promoting crop root development, enhancing drought resistance, salt and alkali resistance and other stress resistance, realizing the integration of "weed control-seedling protection-yield increase", and solving the defects of the single function of existing technologies. 5. The core components of the product are all bio-based substances, fully biodegradable, with no residue in soil or water environments, meeting the requirements of green agricultural development. The formula system is resistant to hard water and high and low temperatures, and its performance remains stable for 12 months at room temperature. Field application is not affected by environmental factors such as temperature and rainfall, and its industrial application value is outstanding.

[0014] A second objective of this invention is to provide a method for preparing the above-mentioned herbicide synergist composition, comprising the following steps: (1) Weigh each component according to its weight percentage; (2) Add deionized water to a closed reaction vessel (reactor), heat to 33℃~37℃, add rhamnolipin and defoamer, stir at 200~250 rpm until completely dissolved, keep warm and stand for 10~20 min; (3) Add complex enzyme glycosides to the reaction vessel (reaction kettle), stir at 200~300 rpm and heat to 45℃~50℃, keep warm and stir until completely dissolved and mixed, cool to room temperature and then add pH adjuster to adjust the pH of the system to 5.8~6.2; (4) After filtration through a 200-300 mesh sieve, a uniform and stable herbicide synergist composition is obtained.

[0015] Furthermore, the pH adjuster is one of the following: citrate-sodium citrate buffer pair (0.2 mol / L, pH 6.0, citric acid monohydrate 2.18 g / L, trisodium citrate dihydrate 49.70 g / L) or potassium dihydrogen phosphate-dipoxatium hydrogen phosphate buffer pair (0.2 mol / L, pH 5.8, potassium dihydrogen phosphate 26.79 g / L, dipotassium hydrogen phosphate trihydrate 1.57 g / L).

[0016] A third object of the present invention is to provide the application of the above-mentioned synergistic adjuvant composition for herbicides, wherein the synergistic adjuvant composition is mixed with the herbicide for immediate use in the field.

[0017] Furthermore, the amount of the synergistic adjuvant composition added is 0.5% to 2.0% of the total mass of the herbicide spray solution.

[0018] Furthermore, the herbicide dosage is 50% to 70% of the conventional recommended field dosage. The herbicide solution with the added synergistic adjuvant composition is applied using conventional backpack sprayers or drone spraying methods for the control of weeds in fields of grain crops, cash crops, and oil crops, achieving a 30% to 50% reduction in herbicide dosage while improving weed control efficacy.

[0019] Furthermore, the herbicide does not contain benzoxazine or its salts, and the herbicide is one or more of the following compound formulations: mesotrione, nicosulfuron, atrazine, quizalofop-P-ethyl, cyhalofop-butyl, glyphosate, glufosinate, triclopyralid, flusulfanilamide, clodinafop-propargyl, and clodinafop-propargyl.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The novel formulation system and application scenarios of this invention are highly innovative: For the first time, the combination of complex enzyme glycosides and rhamnolipids is applied to the field of broad-spectrum herbicide dosage reduction and efficiency enhancement, completely eliminating traditional petrochemical-based wetting and dispersing agents, thus opening up a new research and development direction for bio-based herbicide adjuvants. Existing technologies only disclose the phytotoxicity-relieving effect of complex enzyme glycosides on the single herbicide benzoyl permethrin, without addressing the complex enzyme glycoside-rhamnolipid compound adjuvant composition of this invention, or the synergistic effect of the combination on the broad-spectrum dosage reduction and efficiency enhancement of various herbicides; there are no relevant reports domestically or internationally.

[0021] 2. The synergistic effect of this invention breaks through the bottleneck of existing technology, and the reduction in dosage is significant: Through the synergistic effect of complex enzyme glycosides and rhamnolipids, this invention can effectively improve the herbicidal effect of herbicides, increasing the reduction in herbicide dosage from 20%~30% to 30%~50%, and the control efficacy against weeds and the fresh weight control efficacy are still significantly higher than those of conventional dosage of single herbicides; while in the prior art, complex enzyme glycosides can only slightly improve the control efficacy of single herbicides, without a clear herbicide reduction effect. The synergistic effect of this invention far exceeds that of the prior art, achieving unexpected technical results.

[0022] 3. The present invention has strong broad-spectrum adaptability, which solves the pain point of poor universality of the prior art: it has excellent synergistic effect on different herbicides and is suitable for a variety of crops such as corn, wheat, rice, soybeans, tobacco, fruits and vegetables, completely breaking through the limitation of the prior art that can only be adapted to the single herbicide benzoxazine.

[0023] 4. The invention has multiple functions, realizing the integration of weed control, seedling protection and yield increase: Existing adjuvants only have a single synergistic function, and existing complex enzyme glycoside related technologies can only alleviate the high temperature phytotoxicity of specific herbicides. However, the composition of the present invention, through the synergistic effect of complex enzyme glycosides and rhamnolipids, can not only broadly enhance the weed control effect of various herbicides, but also alleviate the phytotoxicity of various herbicides to crops. At the same time, it promotes crop root development, enhances stress resistance, and achieves quality improvement and yield increase. This is an unexpected technical effect that existing technologies cannot achieve.

[0024] 5. This invention is green and stable, with high industrialization and promotion value: The core components of the composition are all bio-based substances, which are completely biodegradable, leaving no residue or pollution; the formulation system uses a buffer pair to stabilize pH, is resistant to hard water and high and low temperatures, and does not separate or precipitate after 12 months of storage at room temperature, and its field application is not affected by environmental factors; at the same time, this invention is an independent adjuvant, which can be flexibly adapted to the application needs of various herbicides in the field without changing the existing herbicide formulation production process, resulting in low promotion costs. Detailed Implementation

[0025] The principles and features of the present invention are described below with reference to examples. The examples are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0026] In the specific implementation, the complex enzyme glycoside used is a microbial directional fermentation product developed by Yantai Goodlife Biotechnology Co., Ltd., product model FM-22; the rhamnolipid is purchased from Shanghai Maclean Biochemical Technology Co., Ltd., product number R931237, with an effective ingredient content of ≥98%; the defoamer is an organosilicon defoamer, purchased from Sinopharm Chemical Reagent Co., Ltd., product code 30214670.

[0027] Example 1 A method for preparing a herbicide synergist composition includes the following steps: (1) Weigh each component by weight percentage: complex enzyme glycosides 35%, rhamnolipin 8%, organosilicon defoamer 0.5%, deionized water 56.5%; (2) Add deionized water to a sealed reactor, heat to 35°C, add rhamnolipin and organosilicon defoamer, stir at 220 rpm until completely dissolved, and keep warm and stand for 10 min. (3) Add complex enzyme glycosides to the reaction vessel, stir at 200 rpm and heat to 50°C, keep warm and stir until completely dissolved and mixed, cool to room temperature and then add the pH adjuster citrate-sodium citrate buffer pair (0.2 mol / L, pH 6.0, 2.18 g / L citric acid monohydrate and 49.70 g / L trisodium citrate dihydrate) to adjust the pH of the system to 6.0; (4) After filtration through a 300-mesh sieve, a uniform and stable herbicide synergist composition is obtained.

[0028] Example 2 A method for preparing a herbicide synergist composition includes the following steps: (1) Weigh each component by weight percentage: complex enzyme glycoside 10%, rhamnolipin 4%, organosilicon defoamer 0.1%, deionized water 85.9%; (2) Add deionized water to a sealed reactor, heat to 33°C, add rhamnolipin and organosilicon defoamer, stir at 250 rpm until completely dissolved, and keep warm and stand for 20 min. (3) Add the complex enzyme glycoside to the reaction vessel, stir at 300 rpm and heat to 45°C, keep warm and stir until completely dissolved and mixed, cool to room temperature and then add the pH adjuster potassium dihydrogen phosphate-dipoxat phosphate buffer pair (0.2 mol / L, pH 5.8, potassium dihydrogen phosphate 26.79 g / L, dipotassium hydrogen phosphate trihydrate 1.57 g / L) to adjust the pH of the system to 5.8; (4) After filtration through a 200-mesh sieve, a uniform and stable herbicide synergist composition is obtained.

[0029] Example 3 A method for preparing a herbicide synergist composition includes the following steps: (1) Weigh each component by weight percentage: complex enzyme glycoside 11%, rhamnolipin 2%, organosilicon defoamer 0.2%, deionized water 86.8%; (2) Add deionized water to a sealed reactor, heat to 37°C, add rhamnolipin and organosilicon defoamer, stir at 200 rpm until completely dissolved, and keep warm and stand for 15 min. (3) Add complex enzyme glycosides to the reaction vessel, stir at 250 rpm and heat to 48°C, keep warm and stir until completely dissolved and mixed, cool to room temperature and then add the pH adjuster citrate-sodium citrate buffer pair (0.2 mol / L, pH 6.0, 2.18 g / L citric acid monohydrate and 49.70 g / L trisodium citrate dihydrate) to adjust the pH of the system to 6.2; (4) After filtration through a 250-mesh sieve, a uniform and stable herbicide synergist composition is obtained.

[0030] Comparative Example 1 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: complex enzyme glycosides and rhamnolipids are replaced with an equal amount of deionized water, while the remaining steps are the same, to prepare a blank adjuvant composition without complex enzyme glycosides and rhamnolipids.

[0031] Comparative Example 2 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: the complex enzyme glycoside is replaced with an equal amount of rhamnolipin, while the other steps are the same, to prepare a control composition containing only rhamnolipin.

[0032] Comparative Example 3 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: rhamnolipid is replaced with an equal amount of complex enzyme glycoside, while the other steps are the same, to prepare a control composition containing only complex enzyme glycoside.

[0033] Comparative Example 4 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: complex enzyme glycosides and rhamnolipids are replaced with an equal amount of organic surfactant Silwet L-77 (a mainstream organosilicon synergist on the market), while the remaining steps are the same, to prepare a control composition containing organic surfactant.

[0034] Comparative Example 5 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: the complex enzyme glycosides and rhamnolipids are replaced with an equal amount of 3% lecithin-vitamin E suspension (Anrongle), and the remaining steps are the same, to prepare a control composition containing Anrongle.

[0035] Comparative Example 6 A method for preparing a herbicide synergist composition, referring to Example 1, differs from Example 1 in that: complex enzyme glycosides and rhamnolipids are replaced with an equal amount of stimulating adjuvant, while the remaining steps are the same, to prepare a control composition containing the stimulating adjuvant.

[0036] test: Experiment 1: Verification Experiment of Synergistic Effect of Complex Enzyme Glycosides and Rhamnolipids In 2025, a field trial was conducted at a maize planting base in Laiyang, Shandong Province. The soil pH was 6.8, the organic matter content was 1.1%, the maize variety was Zhengdan 958, and the dominant weeds in the field were barnyardgrass, millet, amaranth, and lambsquarters. The previous crop in the experimental field was wheat. This experiment used atrazine (30% nitrosulfuron-atrazine suspension) as the herbicide formulation to verify the synergistic effect of the composition of this invention on atrazine, and at the same time to verify the synergistic effect of complex enzyme glycosides and rhamnolipids.

[0037] The experiment consisted of 11 treatments, each with 3 replicates, and a plot area of ​​20 m². 2 The pesticide was applied in a randomized block design at the 3-5 leaf stage of corn and the 2-4 leaf stage of weeds, using a backpack electric sprayer for uniform spraying, with a water dilution rate of 450 L / hm². 2 The design for each process is as follows: Treatment 1: Conventional dosage of herbicide (150 mL / 667 m 2 (Recommended field dosage) Treatment 2: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 + Example 1 composition (1.0% drug volume, 1.05 mL / 667m) 2 ); Treatment 3: Herbicide dosage reduced by 50% (75 mL / 667m³) 2 + Example 1 composition (1.0% drug volume, 0.75 mL / 667m) 2 ); Treatment 4: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 + Example 2 composition (1.0% drug volume, 1.05 mL / 667m) 2 ); Treatment 5: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 + Example 3 composition (1.0% drug volume, 1.05 mL / 667m) 2 ); Treatment 6: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 ) + Comparative Example 1 composition (drug volume 1.0%, 1.05 mL / 667m 2 ); Treatment 7: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 ) + Comparative Example 2 composition (drug volume 1.0%, 1.05 mL / 667m 2 ); Treatment 8: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2) + Comparative Example 3 composition (drug volume 1.0%, 1.05 mL / 667m 2 ); Treatment 9: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 ) + Comparative Example 4 composition (drug volume 1.0%, 1.05 mL / 667m 2 ); Treatment 10: Herbicide dosage reduced by 30% (105 mL / 667 m³) 2 ) + Comparative Example 5 composition (drug volume 1.0%, 1.05 mL / 667m 2 ); Treatment 11: Herbicide dosage reduced by 30% (105 mL / 667m³) 2 ) + Comparative Example 6 composition (drug volume 1.0%, 1.05 mL / 667m 2 ).

[0038] Survey and measurement methods: Thirty days after application, the number of weeds in each plot was investigated, and the control efficacy per plant was calculated. The fresh weight of weeds in each plot was also investigated, and the fresh weight control efficacy was calculated. Herbicide damage was observed at the corn tasseling stage, and corn yield and yield traits were measured at maturity. Data were analyzed for significance using SPSS 22.0, and the results are shown in Tables 1 and 2 below.

[0039] Table 1. Control efficacy of different treatments on weeds in maize fields (30 days after application)

[0040] Note: Different lowercase letters after the data indicate significant differences between the data. P＜0.05 (The same applies below).

[0041] Table 2. Effects of different treatments on maize yield and safety.

[0042] Analysis of experimental results: 1. Synergistic Effect Verification: The compositions (compound of complex enzyme glycosides and rhamnolipids) of Examples 1-3 of this invention, under the condition of a 30% reduction in herbicide dosage, all achieved a total weed fresh weight control efficacy of over 88%, significantly higher than the conventional herbicide dosage treatment (treatment 1); under the condition of a 50% reduction in herbicide dosage (treatment 3), the total weed fresh weight control efficacy was 84.92%, comparable to the control efficacy (84.75%) of the control group (treatment 1), proving that the synergist prepared by this invention can achieve the desired weed control effect without affecting the weed control efficacy. The amount of herbicide applied was significantly reduced (by 50%). In contrast, the control efficacy of rhamnolipin alone in Comparative Example 2 and the control efficacy of complex enzyme glycosides alone, under the same reduction conditions, was only 75.78% and 77.81% of the total fresh weight of weeds, respectively, which is far lower than that in the embodiments of the present invention. Moreover, the combined control efficacy of the two was still significantly lower than that of the compound group, which fully demonstrates that the compound enzyme glycosides and rhamnolipin have a very strong synergistic effect. The combined effect of the two is far better than that of the single component used alone, and unexpected technical effects have been achieved.

[0043] 2. Compared with existing mainstream adjuvants (comparative examples 4-6), the composition of the present invention has significantly better control efficacy under the same herbicide reduction range, while having no risk of phytotoxicity and yield-increasing effect. Its comprehensive performance far surpasses that of mainstream adjuvants on the market.

[0044] Experiment 2: Broad-spectrum synergistic effect of synergistic adjuvant compositions on different herbicides and compound formulations Field trials were conducted on commonly used single-agent and compound herbicides in wheat fields, rice fields, soybean fields, peanut fields, tobacco fields, orchards, and wastelands to verify the broad-spectrum synergistic effect of the composition of Example 1 of this invention. The experimental design was as follows: conventional herbicide dosage, herbicide dosage reduction of 30%, and herbicide dosage reduction of 30% + composition of Example 1 (1.0% of herbicide solution). The total fresh weight control efficacy was measured 30 days after application, and the results are shown in Table 3 below.

[0045] Table 3 Synergistic effects of adjuvant compositions on herbicides in different crop fields

[0046] The experimental results in Table 3 show that the composition of the present invention has a highly significant synergistic effect on different types of single and compound herbicides in wheat fields, rice fields, soybean fields, peanut fields, tobacco fields, orchards, and wastelands. Under the condition of 30% reduction in herbicide dosage, the control efficacy exceeds 84%, which is significantly higher than that of conventional herbicide dosage treatment. This fully demonstrates that the composition of the present invention has excellent broad-spectrum adaptability and unexpected synergistic effects on various herbicides and compound formulations. It can be widely used for reducing dosage and increasing efficacy of various crops and different types of herbicides.

[0047] Experiment 3: Efficacy verification test of synergistic adjuvant composition against benzoxazolone In 2025, a field trial was conducted at a maize planting base in Laiyang, Shandong Province. The soil, maize variety, weed type, application time, and application method were all the same as in Experiment 1. The only difference was the herbicide used; this experiment used benzoxazole (30% benzoxazole suspension concentrate) as the herbicide to verify the synergistic effect of the composition of this invention on benzoxazole. The experiment included three treatments, each with three replicates, and each plot was 20 m². 2 The randomized block design is as follows: Treatment 1: Conventional dosage of herbicide (10 mL / 667m) 2 (Recommended field dosage) Treatment 2: Herbicide dosage reduced by 30% (7 mL / 667m³) 2 ); Treatment 3: Herbicide dosage reduced by 30% (7 mL / 667m³) 2 + Example 1 composition (1.0% drug volume, 0.07 mL / 667m) 2 ); Survey and measurement methods: Thirty days after application, the number of weeds in each plot was investigated, and the control efficacy per plant was calculated. The fresh weight of weeds in each plot was also investigated, and the fresh weight control efficacy was calculated. Data were analyzed for significance using SPSS 22.0, and the results are shown in Table 4 below.

[0048] Table 4 Effect of the composition on the control efficacy of benzoxazine (30 days after application)

[0049] The experimental results in Table 4 show that after reducing the dosage of benzoxazole by 30%, the control efficacy against grass weeds, broadleaf weeds, and total fresh weight of weeds in treatment 3, which was added with the composition of the present invention, was not significantly different from that in treatment 2, which used benzoxazole alone with a 30% reduction in dosage. Moreover, it was much lower than that in the conventional dosage treatment of benzoxazole (treatment 1). This fully demonstrates that the synergistic adjuvant composition of the present invention has no significant synergistic effect on benzoxazole and its salts. This clearly distinguishes it from the existing technical solution where complex enzyme glycosides are only compatible with benzoxazole. It further highlights the novelty and inventiveness of the combination of complex enzyme glycosides and rhamnolipids in the present invention for synergizing herbicides other than benzoxazole and its salts.

[0050] Test 4: Storage stability test of synergistic adjuvant composition The compositions prepared in Examples 1-3 and the organosilicon adjuvant composition in Comparative Example 4 were stored at room temperature (25°C) and low temperature (0°C) for 12 months, respectively. Examples 1 and Comparative Example 4 were stored at high temperature (54°C) for 14 days, and the appearance changes were observed. The surface tension change rate was measured according to the standard "Guidelines for the Determination of Physicochemical Properties of Pesticides Part 31: Surface Tension of Aqueous Solutions" (NY / T1860.31-2016). The results are shown in Table 5 below.

[0051] Table 5 Results of storage stability tests on the composition

[0052] The test results in Table 5 show that the compositions of the present invention have excellent storage stability under normal temperature, low temperature and high temperature conditions. No stratification, precipitation or crystallization (flocculation) was observed during 12 months of storage at low temperature, normal temperature and 14 days of storage at high temperature, and the surface tension change rate was less than 3.00%. In contrast, the organosilicon additive composition of Comparative Example 4 showed turbid precipitation after 12 months of storage at room temperature and severe stratification failure after 14 days under high temperature conditions. This proves that the storage stability of the compositions of the present invention is far superior to that of existing organosilicon additives and has extremely strong industrial application value.

[0053] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A herbicide synergist composition, characterized in that, By weight percentage, it comprises the following components: complex enzyme glycosides 10%~35%, rhamnolipids 2%~8%, defoamer 0.1%~0.5%, and deionized water 56.5%~86.8%; Among them, the complex enzyme glycoside is a microbial directional fermentation product developed by Yantai Goodlife Biotechnology Co., Ltd., product model FM-22; the rhamnose glycolipid is purchased from Shanghai Maclean Biochemical Technology Co., Ltd., product number R931237, with an effective ingredient content of ≥98%.

2. The herbicide synergist composition according to claim 1, characterized in that, The defoamer is an organosilicone defoamer.

3. A method for preparing a herbicide synergist composition as described in claim 1 or 2, characterized in that, Includes the following steps: (1) Weigh each component according to its weight percentage; (2) Add deionized water to a sealed reaction vessel, heat to 33℃~37℃, add rhamnolipin and defoamer, stir at 200~250 rpm until completely dissolved, keep warm and stand for 10~20 min; (3) Add the complex enzyme glycoside to the reaction vessel, stir at 200~300 rpm and heat to 45℃~50℃, keep warm and stir until completely dissolved and mixed, cool to room temperature and then add pH adjuster to adjust the pH of the system to 5.8~6.2; (4) After filtration through a 200-300 mesh sieve, a uniform and stable herbicide synergist composition is obtained.

4. The preparation method according to claim 3, characterized in that, The pH adjuster is one of the following: citrate-sodium citrate buffer pair or potassium dihydrogen phosphate-dipoxat phosphate buffer pair.

5. The application of a herbicide synergist composition as described in claim 1 or 2, characterized in that, The synergistic adjuvant composition is mixed with the herbicide and used immediately in the field. The herbicide is one or more of the following compound formulations: nicosulfuron, atrazine, quizalofop-P-ethyl, cyhalofop-butyl, glyphosate, glufosinate, triclopyralid, flusulfanilamide, clodinafop-propargyl, and clodinafop-propargyl.

6. The application according to claim 5, characterized in that, The amount of the synergistic adjuvant composition added is 0.5% to 2.0% of the total mass of the herbicide spray solution.

7. The application according to claim 5, characterized in that, The amount of herbicide used is 50% to 70% of the conventional recommended amount for field use.

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