Method for detecting nitrite in blood based on griess derivatization-liquid chromatography tandem mass spectrometry

By combining Griess derivatization with liquid chromatography-tandem mass spectrometry, the problems of matrix interference and complex pretreatment in blood nitrite detection have been solved, achieving highly sensitive and accurate quantitative and qualitative analysis, suitable for complex blood samples.

CN122409894APending Publication Date: 2026-07-17SHENZHEN ACAD OF INSPECTION & QUARANTINE +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN ACAD OF INSPECTION & QUARANTINE
Filing Date
2026-04-28
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for detecting nitrite in blood suffer from problems such as complex blood matrix, severe interference from organic matter and chloride ions, cumbersome pretreatment steps, and insufficient sensitivity, making it difficult to achieve efficient and accurate quantitative and qualitative analysis.

Method used

The Griess derivatization combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to purify the sample and enrich the target analytes by acetonitrile precipitation of proteins, sodium chloride supersaturation salting-out, and pH adjustment with phosphate buffer, followed by detection by HPLC-MS/MS.

Benefits of technology

It enables simple, rapid, sensitive, and highly specific quantitative and qualitative analysis of nitrite in blood, applicable to hemolyzed or putrefied blood samples, broadening the detection range, reducing interference from endogenous substances, and improving the accuracy and sensitivity of detection.

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Abstract

The application discloses a method for detecting nitrite in blood based on Griess derivatization combined with liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). The method utilizes the diazotization reaction of nitrite ions and p-aminobenzenesulfonic acid, and then couples with alpha-naphthylamine to generate a purple red azo compound. Quantitative analysis is realized through liquid chromatography separation and mass spectrometry detection. In view of the problems of complex blood matrix, existence of organic matter and interference of inorganic anions such as chloride ions, the application effectively reduces the matrix interference by optimizing the pretreatment process. Compared with the prior art, the method simplifies the operation steps, has good sensitivity, selectivity and separation effect, can meet the accurate detection requirements of nitrite in complex blood samples, and is suitable for forensic toxicological analysis and related clinical detection fields.
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