Compound functional beverage for improving tissue cell hypoxia and its preparation method and application

Abstract

The application belongs to the technical field of functional drinks, and particularly relates to a compound functional beverage for improving tissue cell hypoxia and a preparation method and application thereof. 12 The compound functional beverage is prepared by using high-oxygen liquid as a base, and compounding vitamin B1, vitamin B2 and vitamin B6, wherein the high-oxygen liquid is prepared by a low-temperature oxygenation or ozone-charging process, can be absorbed through the digestive tract to realize intestinal oxygen delivery, directly improve the blood oxygen partial pressure and blood oxygen saturation of the body, and eliminate lactic acid accumulation; the water-solubility of the B-group vitamins is good, and the chemical properties are stable, so that the B-group vitamins can provide synthesis materials for coenzyme of the mitochondrial respiratory chain, and improve the oxygen uptake and utilization capacity of cells. The two can break the vicious cycle of hypoxia inducible factor-1 alpha and lactic acid, and improve the hypoxia state of tissue cells. The application can improve the blood lactic acid clearance rate of the sports population by 20% to 25%, and improve the blood oxygen saturation under the highland environment by 5% to 8%, and can also be used for hypoxia auxiliary intervention and chemotherapy sensitivity enhancement of tumor radiotherapy and chemotherapy, and is suitable for all kinds of hypoxia population.

Description

Technical Field

[0001] This invention belongs to the field of functional beverage technology, specifically relating to a compound functional beverage that improves tissue cell hypoxia, its preparation method, and its application. Background Technology

[0002] Hypoxia is a common problem in the body under pathological and physiological stress conditions. It can be seen in various scenarios such as exposure to high-altitude environments, high-intensity exercise, and radiotherapy and chemotherapy for tumors. It can have a significant impact on the body's energy metabolism and tissue function. Improving the hypoxic state of tissue cells has important practical value in multiple application scenarios.

[0003] According to classical pathophysiology, hypoxia involves four core processes: first, external respiration, where oxygen is obtained from the external environment through the lungs; second, blood oxygenation, where oxygen combines with blood cells; third, circulatory oxygen transport, where oxygenated blood is pumped to all tissues by the heart; and fourth, tissue oxygen utilization, where tissue cells take up and utilize oxygen. The essence of hypoxia is a decline in the ability of tissue cells to take up and utilize oxygen. At the cellular level, mitochondria are the core organelles for oxygen uptake and utilization, completing a series of biochemical reactions through the respiratory chain, a process requiring the participation of various coenzymes. Current technological approaches to improving cellular hypoxia typically revolve around these four processes.

[0004] Under hypoxic conditions, hypoxia-deducible factor-1α (HIF-1α) inhibits the activity of prolyl hydroxylase (PHD), promotes its own nuclear translocation, and activates downstream target genes, thereby inducing angiogenesis, enhancing glycolysis, and ultimately producing large amounts of lactate. The accumulation of lactate further upregulates the expression level of HIF-1α. Based on this mechanism, related studies have proposed the concept of the HIF-1α / lactic acid functional axis: tissue hypoxia leads to increased lactate production, further exacerbating the acidification of the tissue microenvironment; the acidified microenvironment, in turn, promotes the sustained high expression of HIF-1α in tissues, thereby inducing the production of more lactate, forming a vicious cycle of "hypoxia-lactic acid accumulation-microenvironment acidification-HIF-1α high expression-further lactate production," continuously aggravating the hypoxic state of tissue cells.

[0005] Currently, functional beverages related to improving oxygen deficiency and relieving fatigue are mainly divided into two categories, both of which have significant technical limitations: The first category is conventional energy-boosting and fatigue-relieving functional beverages. These products mostly rely on stimulating ingredients such as caffeine and taurine to achieve a temporary boost, and some products add B vitamins to assist energy metabolism. However, these products cannot directly improve the acidic microcirculation environment caused by oxygen deficiency, nor can they interfere with the oxygen utilization efficiency of cellular mitochondria, and therefore cannot fundamentally improve the hypoxic state of tissue cells.

[0006] The second category is oral hyperoxia products. These products can be absorbed through the digestive tract to achieve "intestinal oxygen delivery," increasing the body's blood oxygen partial pressure and blood oxygen saturation, and to some extent improving the hypoxic microenvironment of tissue cells and promoting lactic acid clearance. However, simply taking oral hyperoxia products can only improve blood oxygen levels from the perspective of oxygen supply; it cannot improve the ability of cellular mitochondria to take up and utilize oxygen, making it difficult to break the vicious cycle of HIF-1α and lactic acid at the level of cellular oxygen utilization. Therefore, its effect on improving tissue cell hypoxia is significantly limited.

[0007] In summary, existing products for improving tissue and cellular hypoxia all suffer from the limitation of acting on only one dimension: conventional functional beverages cannot directly improve the hypoxic microenvironment, and simply taking oral hyperoxia solutions cannot enhance the oxygen utilization capacity of cellular mitochondria. Neither can simultaneously work synergistically at the levels of blood oxygen supply and cellular oxygen utilization, making it difficult to effectively block the vicious cycle of HIF-1α and lactate, and thus failing to fundamentally improve the hypoxic state of tissues and cells in a multidimensional way. Therefore, developing a compound functional beverage that can synergistically improve hypoxia through multiple pathways is a pressing technical problem to be solved in this field. Summary of the Invention

[0008] The purpose of this invention is to provide a compound functional beverage that improves tissue and cell hypoxia, as well as its preparation method and application. This invention overcomes the shortcomings of existing ordinary functional beverages that rely solely on stimulating ingredients for temporary alertness and cannot directly improve hypoxic microcirculation and cellular oxygen utilization efficiency. It also solves the technical defect that simply taking high-oxygen liquid orally cannot enhance the oxygen uptake and utilization capacity of cellular mitochondria.

[0009] The objective of this invention is achieved through the following technical solution: This invention provides a compound functional beverage for improving tissue cell hypoxia, comprising a hyperoxygenated liquid and B vitamins; the B vitamins include vitamin B1, vitamin B2, and vitamin B6. 12 .

[0010] Furthermore, each 500mL of the aforementioned multifunctional beverage contains 1mg of vitamin B1, 1mg of vitamin B2, and 1mg of vitamin B3. 12 2.0 μg.

[0011] Furthermore, the high-oxygen solution is a solution containing high partial pressure dissolved oxygen, prepared by using Ringer's solution or physiological saline as the base liquid and through a low-temperature oxygenation or ozone filling process.

[0012] The present invention also provides a method for preparing the aforementioned compound functional beverage for improving tissue cell hypoxia, comprising the following steps: (1) A high-oxygen liquid is prepared by using the base liquid as raw material and then filling it with oxygen or ozone at low temperature; (2) Add vitamin B1, vitamin B2 and vitamin B to the oxygenated liquid. 12 Stir until the components are completely dissolved, and mix well to obtain the compound functional beverage.

[0013] Further, step (1) specifically involves: placing the base liquid in a refrigerator for 12 hours, taking it out, and then introducing ozone at room temperature of 20°C for 10 minutes to obtain a high oxygen liquid with a dissolved oxygen concentration of 20 mg / L. After preparation, the liquid is placed in a refrigerator for later use.

[0014] Furthermore, the base solution is Ringer's solution or physiological saline.

[0015] The present invention also provides an application of the aforementioned compound functional beverage in the preparation of a beverage for improving the hypoxic state of body tissue cells.

[0016] Furthermore, the beverage can improve blood oxygen saturation and increase the rate of blood lactic acid clearance for people with high altitude hypoxia or those experiencing hypoxia during exercise.

[0017] Furthermore, the beverage, when used as an adjunct intervention for patients undergoing radiotherapy and chemotherapy for tumors, can downregulate the expression of hypoxia-inducible factor-1α and the multidrug resistance gene MDR1, thereby enhancing the sensitivity to chemotherapy drugs.

[0018] Furthermore, the beverage is used to enhance the body's ability to resist fatigue and prolong the duration of exhaustive exercise.

[0019] The beneficial effects of this invention are as follows: This invention provides a compound functional beverage for improving tissue and cellular hypoxia. Through the synergistic combination of a hyperoxygenated liquid and B vitamins, it works by addressing two core aspects: oxygen transport and supply within the body, and oxygen utilization at the cellular level, effectively improving the hypoxic state of tissues and cells. The hyperoxygenated liquid can be absorbed through the digestive tract to achieve "intestinal oxygen delivery," directly increasing the body's blood oxygen partial pressure and saturation, rapidly improving the hypoxic microenvironment of tissues and cells, and promoting the elimination of lactic acid buildup. The compounded vitamins B1, B2, and B vitamins... 12Both components are water-soluble and chemically stable, capable of remaining stable in a hyperoxygenated aqueous system. They provide key raw materials for the synthesis of coenzymes related to the mitochondrial respiratory chain, enhancing the activity and quantity of these coenzymes, activating mitochondrial respiratory chain function, and improving oxygen uptake and utilization efficiency at the cellular level. The combined effect of these two components effectively breaks the vicious cycle between hypoxia-inducible factor-1α and lactic acid under hypoxic conditions. By improving acidic microcirculation, they downregulate the expression of hypoxia-inducible factor-1α, thereby reducing lactic acid production and accumulation. This fundamentally improves the hypoxic state of tissue cells, overcoming the shortcomings of existing ordinary energy drinks that rely solely on stimulating ingredients for temporary alertness and cannot directly improve hypoxic microcirculation and cellular oxygen utilization efficiency. It also solves the technical deficiency that simply taking hyperoxygenated liquid orally cannot improve the oxygen uptake and utilization capacity of cellular mitochondria.

[0020] Experimental results have verified that this compound functional beverage significantly improves hypoxia and combats fatigue compared to single-ingredient products. In hypoxic exercise scenarios, drinking this beverage can increase the rate of lactic acid clearance in athletes by 20%-25%, rapidly alleviating fatigue and hypoxia caused by lactic acid buildup after exercise. In high-altitude hypoxic environments, it can increase blood oxygen saturation by 5%-8%, effectively reducing hypoxia induced by high-altitude conditions. Further results from mouse exhaustive swimming experiments confirm that the combination of hyperoxia solution with vitamins B1 and B2 has a clear synergistic effect, prolonging the exhaustive swimming time of mice far more effectively than using hyperoxia solution alone or in combination with a single B vitamin. Furthermore, both the use of hyperoxia solution alone and the combination of the three products show a clear dose-dependent relationship, with the anti-fatigue and hypoxia tolerance effects increasing with increasing dosage, allowing for flexible dosage adjustments based on different usage scenarios and intervention needs.

[0021] In addition to improving hypoxia under physiological conditions, this multifunctional beverage can also be used as an adjunct intervention in pathological hypoxic scenarios. Targeting tissue hypoxia during tumor radiotherapy and chemotherapy, this multifunctional beverage can effectively increase the local blood oxygen partial pressure and pH value in tumor tissue, improve the hypoxic acidification state of the tumor microenvironment, and simultaneously downregulate the expression levels of hypoxia-inducible factor-1α and the multidrug resistance gene MDR1. While improving tissue hypoxia, it enhances the sensitivity of tumor cells to chemotherapy drugs, inhibits tumor growth, and provides a safe and feasible oral intervention for improving hypoxia and enhancing chemotherapy sensitization in patients undergoing tumor radiotherapy and chemotherapy. This multifunctional beverage is suitable for a wide range of populations, including those experiencing high-altitude hypoxia, exercise-induced hypoxia, and those undergoing tumor radiotherapy and chemotherapy, all of whom face tissue and cellular hypoxia issues. Detailed Implementation

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0024] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0025] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0026] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0027] Example 1: Preparation of a compound functional beverage for improving tissue cell hypoxia 1. Raw material formula Based on 500mL of the finished functional beverage, it contains the following components: 500mL of high-oxygen liquid, 1mg of vitamin B1, 1mg of vitamin B2, and 1mg of vitamin B2. 12 2.0 μg.

[0028] 2. Preparation of high-oxygen liquid In this embodiment, the hyperoxia solution is prepared using Ringer's solution as a base solution through a low-temperature oxygenation or ozone-filling process. Ringer's solution can play a physiological role in replenishing body fluids and correcting electrolyte imbalances; the high partial pressure dissolved oxygen contained in the hyperoxia solution can be absorbed directly into the bloodstream through the digestive tract, improving the hypoxic microenvironment of tissue cells and promoting the elimination of lactic acid accumulation.

[0029] 3. Finished Product Preparation Process Accurately weigh out vitamin B1, vitamin B2, and vitamin B1 according to the above proportions. 12Add the mixture to the prepared high-oxygen liquid and stir continuously until all components are completely dissolved. After mixing, the compound functional beverage that improves tissue cell hypoxia is obtained.

[0030] Among them, vitamin B1, vitamin B2, and vitamin B1 12 All three are water-soluble vitamins with stable chemical properties and can exist stably in high-oxygen liquid aqueous systems. The three B vitamins mentioned above are key substances for the synthesis of coenzymes related to the mitochondrial respiratory chain. They can increase the activity and quantity of coenzymes in the mitochondrial respiratory chain, activate the function of the mitochondrial respiratory chain, reduce blood lactate concentration, and thus enhance the cell's resistance to hypoxia.

[0031] Example 2: The effect of hyperoxia combined with B vitamins on hypoxia improvement and chemotherapy sensitization in drug-resistant breast cancer cell implantation tumors. This embodiment verifies the effect of hyperoxia combined with vitamin B1 and vitamin B2 on improving the hypoxic state of tumors undergoing chemotherapy and its chemosensitizing effect.

[0032] 1. Experimental Materials and Methods Human breast cancer cisplatin-resistant MCF7 / DDP cells were inoculated into female nude mice to construct a tumor-bearing animal model. The successfully modeled nude mice were divided into an experimental group (group A) and a control group (group B). Both groups of nude mice were given one cisplatin chemotherapy. The control group was given saline intervention, while the experimental group was given hyperoxia combined with vitamin B1 and vitamin B2 intervention.

[0033] During the experimental period, the tumor microenvironment pH, blood oxygen partial pressure (PO2), relative expression of hypoxia-deducible factor-1α (HIF-1α), relative expression of multidrug resistance gene MDR1, as well as tumor tissue diameter and tumor quality were measured in two groups of nude mice.

[0034] 2. Experimental Results Compared with the control group, the experimental group of nude mice had higher tumor microenvironment pH and blood oxygen partial pressure, and the differences were statistically significant (P<0.05). The relative expression levels of HIF-1α and MDR1 in the tumor tissue of nude mice in the experimental group were significantly lower than those in the control group. At the same time, the tumor volume and tumor mass were also lower in the experimental group than those in the control group. The differences between the groups of the above indicators were statistically significant (P<0.05).

[0035] 3. Experimental Conclusions Experimental results show that hyperoxia combined with vitamin B1 and vitamin B2 intervention can effectively improve the hypoxic state of tumor tissue in nude mice with breast cancer drug-resistant cell implantation, while downregulating the expression of hypoxia-inducible factor HIF-1α and multidrug resistance gene MDR1, breaking the vicious cycle of HIF-1α and lactate, thereby enhancing the sensitivity of tumor cells to chemotherapeutic drugs and inhibiting tumor growth.

[0036] Example 3: Effects of hyperoxygenated solution combined with B vitamins on the fatigue resistance and hypoxia tolerance of mice. This embodiment uses a mouse exhaustive swimming experiment to verify the effect of hyperoxia combined with B vitamins in enhancing the body's anti-fatigue and improving hypoxia.

[0037] 1. Experimental Materials and Methods (1) Laboratory animals and grouping Fifty two-week-old male Kunming mice were selected and purchased from an experimental animal supplier in Hunan Province.

[0038] The experimental mice were divided into 5 main groups: saline control group, hyperoxia group, hyperoxia + vitamin B1 group, hyperoxia + vitamin B2 group, and hyperoxia + vitamin B1 + vitamin B2 group. Each main group was further divided into 3 subgroups according to the frequency of administration by gavage once, twice or three times a day: low dose, medium dose and high dose.

[0039] (2) Preparation of test samples Preparation of high-oxygen solution: Physiological saline was placed in a refrigerator for 12 hours, and then ozone was introduced at room temperature of 20°C for 10 minutes. The dissolved oxygen concentration was measured to be 20 mg / L. After preparation, the solution was kept in a refrigerator for later use.

[0040] Hyperoxygenated solution + Vitamin B1 solution: Vitamin B1 was added to the above hyperoxygenated solution to prepare a test solution with a vitamin B1 concentration of 5 mg / L.

[0041] Hyperoxygenated solution + Vitamin B2 solution: Vitamin B2 was added to the above hyperoxygenated solution to prepare a test solution with a vitamin B2 concentration of 1 mg / L.

[0042] Hyperoxygenated solution + Vitamin B1 + Vitamin B2 solution: Vitamin B1 and Vitamin B2 are added to the above hyperoxygenated solution simultaneously, so that the concentration of Vitamin B1 in the solution is 5 mg / L and the concentration of Vitamin B2 is 1 mg / L.

[0043] (3) Test of swimming time at exhaustion Referring to the previously reported method of weight-bearing swimming experiment in mice (Chen Liyi, Li Yuxin, Huang Beiyuan, et al. Experimental study on the anti-hypoxia and anti-fatigue effects of Shenfu injection on mice [J]. Chinese Journal of Emergency Traditional Chinese Medicine, 2015, 24(6): 960). 961,978.), 8 mice were selected from the saline control group and the experimental groups of each dose, for a total of 40 mice to carry out the experiment.

[0044] A swimming tank with dimensions of 50cm×50cm×40cm was used, with a water depth of no less than 30cm. The water temperature was controlled at (25±1.0)℃ during the experiment. 30 minutes after the last administration of the test sample, a lead sheet equivalent to 5% of the mouse's body weight was applied to the base of the mouse's tail. The mouse was then placed in a glass swimming pool to begin swimming, and the time from entry into the water to exhaustion was recorded. The criterion for exhaustion was that the mouse's head was submerged for 7 seconds and no longer floated.

[0045] (4) Statistical analysis All experimental data are expressed as mean ± standard deviation (x ± pm s) and statistically processed using SPSS 18.0 software. One-way ANOVA was used for comparison of multiple groups, and LSD-t test was used for pairwise comparisons between groups. The significance level was α = 0.05.

[0046] 2. Experimental Results The overall variance analysis results of the exhaustive swimming time of mice in each group showed that: F(12,65)=12.70, P=7.71×10 - ¹³ (P<0.001) indicates that there are extremely significant differences in the exhaustive swimming time among the groups of mice.

[0047] The LSD method was used for pairwise comparisons between groups, and the results are as follows: ① Compared with the saline control group: the exhaustive swimming time of mice in each of the hyperoxia groups alone, the hyperoxia combined with vitamin B1 groups, and the hyperoxia combined with vitamin B2 groups was significantly prolonged (P<0.05); the swimming time of mice in the low, medium, and high dose groups of hyperoxia combined with vitamin B1 and vitamin B2 was significantly longer than that of the saline control group (P<0.01).

[0048] ② Compared with the single-dose groups at the same dose: the low, medium and high dose groups of hyperoxia + vitamin B1 + vitamin B2 showed that the exhaustion swimming time of mice was significantly longer than that of the single hyperoxia group, the hyperoxia + vitamin B1 group and the hyperoxia + vitamin B2 group at the same dose (all P<0.05), which confirms that the combination of hyperoxia and vitamin B1 and vitamin B2 has a synergistic effect in anti-fatigue and improving hypoxia tolerance.

[0049] ③ Dose-response relationship: In the hyperoxygenated liquid monotherapy group and the three-drug combination group, the exhaustion swimming time of mice gradually increased with the increase of the drug dose. The differences between low, medium and high doses within the group were statistically significant (P<0.05); in the groups with only vitamin B1 or only vitamin B2, there was no significant difference in exhaustion swimming time among different dose groups (P>0.05).

[0050] 3. Experimental Conclusions The mice in the saline control group had the shortest exhaustive swimming time, which was 13.23±3.26. The exhaustive swimming time of mice in each dose group, whether the hyperoxia solution was used alone or in combination with vitamin B1 or vitamin B2, was higher than that of the saline control group, but the increase was limited. The low / medium / high dose groups of the combination of hyperoxia solution, vitamin B1 and vitamin B2 showed a significant increase in the mean exhaustive swimming time of mice, which was much higher than that of all the single-drug groups.

[0051] The dose-response relationship results showed that the anti-fatigue effect of the hyperoxygenated liquid alone increased with increasing dose, showing a dose-dependent relationship of low dose < medium dose < high dose; the effect of the three-drug combination group also showed a significant dose-dependent relationship, showing a change pattern of low dose < medium dose < high dose; when vitamin B1 or vitamin B2 was added alone, the dose-response relationship was not obvious.

[0052] Example 4: Verification of Human Application Effects Human trials have verified that drinking the compound functional beverage described in this invention can increase the blood lactate clearance rate of athletes by 20%-25%, which can quickly alleviate hypoxia and fatigue caused by exercise. In high-altitude environments, drinking this beverage can increase the blood oxygen saturation of the human body by 5%-8%, effectively improving the hypoxia response at high altitudes.

[0053] This multifunctional beverage achieves "intestinal oxygenation" by absorbing high-oxygen liquid through the digestive tract, directly improving blood oxygen levels. At the same time, it is combined with B vitamins to regulate mitochondrial oxygen utilization. The dual pathways work synergistically, making it suitable for people with tissue and cell hypoxia, such as those with high-altitude hypoxia, those with exercise-induced hypoxia, and patients undergoing radiotherapy and chemotherapy for tumors.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A compound functional beverage for improving tissue cell hypoxia, characterized in that, It includes a high-oxygen liquid and B vitamins; the B vitamins include vitamin B1, vitamin B2, and vitamin B6. 12 .

2. The multifunctional beverage according to claim 1, characterized in that, Each 500mL of the aforementioned multifunctional beverage contains 1mg of vitamin B1, 1mg of vitamin B2, and 1mg of vitamin B3. 12 2.0 μg.

3. The multifunctional beverage according to claim 1, characterized in that, The high-oxygen solution is a solution containing high partial pressure dissolved oxygen, prepared by using Ringer's solution or physiological saline as the base liquid and through a low-temperature oxygenation or ozone filling process.

4. A method for preparing the compound functional beverage for improving tissue cell hypoxia as described in claim 1, characterized in that, Includes the following steps: (1) A high-oxygen liquid is prepared by using the base liquid as raw material and then filling it with oxygen or ozone at low temperature; (2) Add vitamin B1, vitamin B2 and vitamin B to the oxygenated liquid. 12 Stir until the components are completely dissolved, and mix well to obtain the compound functional beverage.

5. The preparation method according to claim 4, characterized in that, The specific steps (1) are as follows: the base liquid is placed in a refrigerator for 12 hours, and after being taken out, ozone is introduced at room temperature of 20°C for 10 minutes to obtain a high oxygen liquid with a dissolved oxygen concentration of 20 mg / L. After preparation, it is placed in a refrigerator for later use.

6. The preparation method according to claim 4, characterized in that, The base solution is Ringer's solution or physiological saline.

7. The use of the compound functional beverage according to claim 1 in the preparation of a beverage for improving the hypoxic state of body tissue cells.

8. The application according to claim 7, characterized in that, The beverage is intended for people with high altitude hypoxia or those experiencing hypoxia during exercise, as it can increase blood oxygen saturation and improve the rate of blood lactate clearance.

9. The application according to claim 7, characterized in that, The beverage is used as an adjunct intervention for patients undergoing radiotherapy and chemotherapy for tumors. It can downregulate the expression of hypoxia-inducible factor-1α and the multidrug resistance gene MDR1, thereby enhancing the sensitivity to chemotherapy drugs.

10. The application according to claim 7, characterized in that, The beverage is used to enhance the body's ability to resist fatigue and prolong the duration of strenuous exercise.