A method for capturing and directly thermal desorption GC-MS analysis of gas and particle phase components of single puff cigarette smoke

Abstract

The application discloses a method for capturing and directly analyzing GC-MS of gas and particle phase components of single puff cigarette smoke. A detachable thermal desorption filter is arranged in the smoke collector, and a thermal desorption adsorption tube is connected downstream of the smoke collector. When the cigarette combustion line reaches the preset position, a puff is taken, so that the particulate matter is intercepted by the filter, and the gas phase components passing through the filter are captured by the adsorption tube. The filter is rolled and placed into the thermal desorption empty tube to form a filter thermal desorption tube. The filter thermal desorption tube and the gas phase adsorption tube are not subjected to solvent extraction, and the same thermal desorption, cold trap and GC-MS conditions are used for determination by adding internal standards, so that the data of the gas and particle phase distribution of single puff cigarette smoke are obtained. The method can reduce the loss of components and the migration between phases caused by multiple puffs and sample pretreatment, and improve the detection capacity of trace components of single puff cigarette smoke and the comparability of two-phase data.

Description

Technical Field

[0001] This invention relates to the field of cigarette smoke analysis technology, specifically to a method for capturing the gas phase and particulate phase components of a single-puff mainstream smoke generated at a specific combustion location of a cigarette in series, and then directly performing thermal desorption, cold trap enrichment, and gas chromatography-mass spectrometry analysis on the components respectively. Background Technology

[0002] Cigarette smoke is a complex mixture formed by the high-temperature combustion and pyrolysis of tobacco. The smoke consists of two phases: a gas phase and a particulate phase. Cambridge filters are used to separate the smoke; the portion trapped by the filter is defined as the "particulate phase," while the portion that passes through the filter is called the "gas phase." A large number of volatile and semi-volatile components are distributed in both the gas and particulate phases. Typical examples include moisture and volatile organic compounds with a relative molecular mass ranging from 60 to 200, which exhibit characteristics of both phases. The content and distribution characteristics of gas and particulate components in cigarette smoke are core indicators characterizing the physicochemical properties of cigarette smoke, directly determining its formation mechanism, transport and diffusion patterns, and component transformation behavior. Furthermore, the effects of gas and particulate components on human senses differ significantly: gas components can directly contact the oral mucosa, have a faster rate of action, and have a more direct and significant impact on the smoking experience. Studying the content levels and distribution of gaseous and particulate components in mainstream cigarette smoke is of great significance for clarifying the evolution mechanism of cigarette smoke components and improving the sensory quality evaluation system of cigarettes.

[0003] Currently, research on the particulate phase composition of mainstream tobacco smoke mainly focuses on the mainstream smoke produced by the combustion of an entire cigarette. Methods for analyzing the particulate phase composition of tobacco smoke typically involve continuously puffing through the cigarette to capture the particulate phase components of the mainstream smoke, which are then extracted and processed for instrumental analysis. Because the content of gaseous components in cigarette smoke is relatively low, in order to meet the requirements of detection sensitivity, gas-particle phase component analysis often requires the cumulative collection of mainstream smoke from multiple cigarettes. For example, Li Junjie et al. (Analysis of the full composition of mainstream cigarette smoke by gas chromatography-mass spectrometry based on filter-adsorption tube tandem collection. Physicochemical Testing - Chemical Analysis, 2025, 61(09): 1009-1024) used Cambridge filters and adsorption tubes to continuously collect particulate and gaseous components of mainstream cigarette smoke from 20 cigarettes, and after extraction, analyzed them by GC-MS to clarify the content of gas-particle components in mainstream cigarette smoke; Wu Bingyu et al. (The effect of filter ventilation on the release of gaseous and particulate components in mainstream cigarette smoke. Tobacco Technology, 2024, 57(12): (25-32) Using similar collection methods, the mainstream smoke of 20 cigarettes was cumulatively collected, and the effect of filter ventilation on the gas-particle phase composition was investigated. Sun Yuli et al. (Series cold trap collection-gas chromatography / mass spectrometry analysis of gas phase composition of mainstream cigarette smoke. Tobacco Science and Technology, 2016, 49(3): 52-61) established a series cold trap collection-GC-MS method to realize the analysis of gas phase composition of mainstream smoke of 20 cigarettes. In addition, Qin Yaqiong et al. (Difference analysis of volatile and semi-volatile components in mainstream smoke of cigarette leaf and stem. Tobacco Science and Technology, 2019, 52(09): 80-90) used Cambridge filters and absorption bottles to collect the gas-particle phase of mainstream smoke of 8 cigarettes and completed the analysis of volatile and semi-volatile components.

[0004] However, in the actual smoking process, the smoker's sensory experience does not come from the cumulative effect of the entire cigarette's smoke, but rather from the fresh smoke produced by each puff, i.e., single-puff smoke. Existing whole-smoke analysis ignores the actual "single-puff" smoking scenario, and the resulting particulate phase distribution data is an average of multiple cigarettes and multiple puffs. When the filter accumulates and captures smoke over multiple puffs, the particulate components on the filter are repeatedly washed away during subsequent smoking and partially transferred to the gas phase, resulting in a particulate phase distribution that is not that of "fresh" smoke. Furthermore, the particulate matter trapped on the filter may alter its retention efficiency for subsequent smoke particles. Therefore, particulate phase composition analysis based on multiple cigarettes and multiple puffs of smoke capture cannot truly reflect the particulate phase state of the smoke components during each puff.

[0005] To achieve the analysis and determination of the particulate phase components of a single puff of "fresh" cigarette smoke, on the one hand, the analytical method needs to simplify the pretreatment steps as much as possible to reduce the loss of smoke components and their possible chemical reactions; on the other hand, the analytical method needs to have high sensitivity to achieve the direct detection of trace components in a single puff of smoke.

[0006] Therefore, there is a need for a method that can separate the gas and particulate phases in a collection path with a low dead volume for single-puff smoke generated at a specific combustion location of cigarettes, and enable both particulate and gaseous samples to enter the same analytical platform with a high effective transfer ratio, thereby improving the detection capability of trace, ultra-trace volatile and semi-volatile components in single-puff smoke. Summary of the Invention

[0007] The purpose of this invention is to provide a tandem trapping and direct thermal desorption GC-MS analysis method for the gas-particle phase components of mainstream cigarette smoke in a single puff. This method can achieve high sensitivity and high accuracy detection of gas-particle phase components in "fresh" cigarette smoke in a single puff. It can reduce the averaging of smoke state and interphase migration caused by cumulative trapping from multiple puffs, reduce losses caused by solvent extraction and sample transfer, and obtain gas phase quantity, particle phase quantity, and gas-particle phase distribution data of the same puff of smoke under consistent thermal desorption and GC-MS conditions.

[0008] The mechanism of this invention lies in the fact that the key to achieving single-aperture flue gas particulate phase composition analysis is how to improve the sensitivity of the method. Under the premise of low single-aperture flue gas component release, increasing the sample volume of components in GC-MS is an important way to improve the component detection rate. After traditional Cambridge filters capture particulate matter, they are usually immersed in solvent (>8 mL) and extracted, and then a small amount of extract (<2 μL) is introduced into GC-MS. The actual sample volume is only less than 1 / 1000 of the total components of a single-aperture flue gas, making most components undetectable by the GC-MS instrument. This invention employs thermal desorption filters and thermal desorption adsorption tubes to separately capture particulate and gaseous components of cigarette smoke from a single puff, reducing the dead volume of the smoke in the collection unit. By combining the thermal desorption filter collector with the thermal desorption adsorption tube, an online collection unit for both particulate and gaseous phases of the smoke is constructed, and direct transfer thermal desorption GC-MS analysis is performed. This ensures that all particulate and gaseous components of the smoke from a single puff are fully introduced into the GC-MS, and the higher sample volume improves the detection rate of the smoke components.

[0009] To achieve the above objectives, the technical solution adopted by the GC-MS analysis method for capturing and directly thermally desorbing particulate phase components of cigarette smoke in this invention is as follows: A GC-MS method for capturing and directly thermally desorbing particulate phase components of cigarette smoke in a single puff includes the following steps: (1) Construct a flue gas gas-particulate phase series online capture unit. The online capture unit includes a flue gas collector and a thermal desorption adsorption tube connected in sequence along the flue gas flow direction. The flue gas collector is provided with a detachable thermal desorption filter for trapping particulate matter. The thermal desorption adsorption tube is provided with an adsorbent for capturing gas phase components passing through the thermal desorption filter. (2) Determine the preset position of the combustion line on the cigarette, connect the cigarette to the gas particle phase series capture unit and ignite it to make it smolder. When the combustion line reaches the preset position, trigger the smoking machine to suck the cigarette once, so that the smoke generated by this sucking passes through the thermal desorption filter and the thermal desorption adsorption tube in sequence, so that the particulate matter is intercepted by the thermal desorption filter and the gas phase components passing through the thermal desorption filter are captured by the adsorbent. (3) After completing the first suction, take out the thermal desorption filter, roll it up and put it into the thermal desorption tube to form a filter thermal desorption tube, wherein the maximum outer diameter of the rolled thermal desorption filter is smaller than the inner diameter of the thermal desorption tube. (4) Add internal standard to the thermal desorption tube of the filter and the thermal desorption adsorption tube containing gas phase components respectively, and perform determination under the same thermal desorption conditions, cold trap trapping conditions and GC-MS analysis conditions without solvent extraction of the thermal desorption filter and the adsorbent. (5) Based on the measurement results of the filter thermal desorption tube and the thermal desorption adsorption tube, obtain the single-particle phase distribution data of the target component in the smoke in this sampling.

[0010] Furthermore, the thermal desorption filter is a glass fiber filter or a quartz fiber filter.

[0011] Furthermore, the inner diameter of the thermal desorption tube is 4-8 mm, the outer diameter of the thermal desorption filter is 15 mm, and the thermal desorption filter is made by punching or cutting a Cambridge filter with an outer diameter of 44 mm.

[0012] Furthermore, the adsorbent in the thermal desorption adsorption tube includes at least two adsorbent materials selected from styrene / divinylbenzene adsorbent and methyl methacrylate / divinylbenzene adsorbent to increase the variety of adsorbed gaseous components.

[0013] Furthermore, the preset position of the combustion line is determined by drawing a line on the cigarette paper; the single inhalation adopts the ISO inhalation mode, the inhalation waveform is bell-shaped, the inhalation volume is 35 mL, and the inhalation duration is 2 s.

[0014] Furthermore, the thermal desorption adsorption tube is aged at 290°C for 120 min under high-purity nitrogen protection before use; after the first suction is completed, both ends of the filter thermal desorption tube and the thermal desorption adsorption tube are sealed respectively.

[0015] In step (4), 1 μL of n-heptadecane internal standard solution with a concentration of 100 μg / mL is added to the filter thermal desorption tube and the thermal desorption adsorption tube, respectively. The solvent of the internal standard solution is methanol or n-hexane.

[0016] In step (4), the thermal desorption conditions are: initial temperature 40℃, heating to 280℃ at 200℃ / min and holding for 9min, and transmission line temperature 280℃; the cold trap trap conditions are: cold trap temperature -100℃, heating to 280℃ at 12℃ / s and holding for 3min.

[0017] In step (4), the GC-MS analysis uses a DB-5 capillary column with helium as the carrier gas at a flow rate of 1.0 mL / min. The initial column temperature is 40℃ and held for 3 min, then increased to 260℃ at a rate of 2℃ / min and held for 10 min. The mass spectrometry uses EI ionization with an ionization energy of 70 eV and an ion source temperature of 230℃. Detection is performed using full scan mode and / or selected ion monitoring mode.

[0018] Furthermore, mass spectrometry was used to preliminarily identify the target components and retention indices were used for qualitative confirmation using standard substances; quantification was performed using an internal standard method with a series of standard solutions.

[0019] Compared with the prior art, the method of the present invention has the following superior effects: This invention employs a thermal desorption filter and a thermal desorption adsorption tube to separately capture the particulate and gaseous phases of cigarette smoke from a single puff, followed by direct transfer thermal desorption GC-MS analysis. This ensures that all particulate and gaseous components of the single-puff smoke are fully incorporated into the GC-MS, avoiding the averaging of instantaneous gas-particle phase states by multi-puff data accumulation. This results in high sensitivity and enables the detection of trace and ultra-trace components in both particulate and gaseous phases of single-puff smoke. The thermal desorption filter and thermal desorption adsorption tube are connected in series, allowing the single-puff smoke to undergo particulate retention followed by gaseous phase capture within the same flow path, reducing differences in smoke quality caused by different sampling operations. Both the thermal desorption filter and the thermal desorption adsorption tube utilize consistent thermal desorption, cold trap, and GC-MS analysis conditions, enabling the analysis of both gaseous and particulate phases of cigarette smoke under the same thermal desorption GC-MS conditions. This helps reduce systemic differences between the two analysis procedures and improves the comparability of gas-particle phase distribution data, resulting in accurate gas-particle phase distribution data for single-puff cigarette smoke. Examples show that this method can qualitatively and quantitatively analyze multiple volatile and semi-volatile components in a single flue gas, with good linearity, recovery rate and precision. Attached Figure Description

[0020] Figure 1This is a schematic diagram illustrating the principle of a method for capturing and directly thermally desorbing particulate phase components of cigarette smoke in Embodiment 1 of the present invention; wherein, the reference numerals are as follows: 1-cigarette; 2-smoke collector; 3-thermal desorption filter; 4-thermal desorption adsorption tube; 41-empty thermal desorption tube; 42-adsorbent; 5-smoking machine; 6-thermal desorption chamber; 7-cold trap; 8-GC-MS instrument.

[0021] Figure 2 This is a GC-MS full scan image of the gas phase and particulate phase of a cigarette smoke from a single puff in Embodiment 3 of the present invention.

[0022] Figure 3 This is a GC-MS SIM image of the gas phase and particulate matter in a single puff of smoke from a cigarette in Example 3 of the present invention. Detailed Implementation

[0023] The technical solution of the present invention will be further described below with reference to specific embodiments. Example 1

[0024] This embodiment presents a GC-MS method for capturing and directly thermally desorbing particulate phase components of cigarette smoke in a single puff, employing, as follows: Figure 1 The schematic diagram shown is intended to more clearly illustrate the method steps of the present invention for single-puff smoke analysis of cigarettes. Figure 1 The method and principle are illustrated with a schematic diagram.

[0025] The present invention first requires the construction of an online collection unit for the particulate matter of a single puff of cigarette smoke (1) during smoking. The collection unit includes a particulate matter collection part and a gaseous matter collection part. The particulate matter collection part is a smoke collector (2), which is equipped with a thermal desorption filter (3) inside. The gaseous matter collection part is a thermal desorption adsorption tube (4), which is composed of a thermal desorption tube (41) filled with an adsorbent (42). The collection unit collects the particulate matter and gaseous matter of a single puff of smoke separately through the thermal desorption filter (3) and the adsorbent (42). The steps of this method are as follows: insert the cigarette (1) into the smoke trap (2), ignite the cigarette (1) to make it smolder, and when its combustion line reaches the preset position, manually trigger the smoking machine (5) to perform single-puff smoking of the cigarette (1) in a specific smoking mode. After single-puff smoking, roll the thermal desorption filter (3) containing particulate matter of the smoke and place it in the thermal desorption tube (41) to form the filter thermal desorption tube. Then transfer the filter thermal desorption tube to the thermal desorption chamber (6) for thermal desorption, cold trap (7) collection and GC-MS (8) analysis. Similarly, transfer the thermal desorption adsorption tube (4) containing gaseous matter of the smoke to the thermal desorption chamber (6) for thermal desorption, cold trap (7) collection and GC-MS (8) analysis, so as to realize the analysis of gaseous and particulate matter of cigarette single-puff smoke under the same thermal desorption conditions and GC-MS analysis conditions, and obtain accurate gas-particle phase distribution data of cigarette single-puff smoke. Example 2

[0026] This embodiment presents a GC-MS method for capturing and directly thermally desorbing particulate phase components of cigarette smoke in a single puff. The specific method operation and evaluation are as follows: (1) Preparation of the capture unit Before use, the thermal desorption tube needs to be aged, that is, under the protection of high-purity nitrogen, the adsorption tube is heated to 290°C, aged for 120 minutes, and then cooled for use.

[0027] Thermal desorption filters were prepared directly from standard Cambridge filters (44 mm outer diameter) using a φ15 mm perforating pliers. These filters could be easily rolled up and placed inside the thermal desorption tube. Two types of adsorbent packing materials, styrene / divinylbenzene and methyl methacrylate / divinylbenzene, were selected and filled into the thermal desorption tube.

[0028] Connect the flue gas collector and the thermal desorption adsorption tube to construct an online flue gas particle phase collection unit.

[0029] (2) Cigarette single-puff smoke capture Draw lines at specific locations on the cigarette paper to determine the preset position of the combustion line. Insert the cigarette into the smoke trap, ignite it, and allow it to smolder. The combustion line slowly moves towards the filter tip. When the combustion line reaches the preset position, manually trigger the smoking machine in ISO mode (bell-shaped suction waveform, 35 mL suction capacity, and 2 s suction duration) to perform a single puff. After the single puff, remove the thermal desorption filter containing particulate matter from the trap, roll it, and place it in the thermal desorption tube to form a filter thermal desorption tube. Seal both ends of the tube, and it is ready for testing. Similarly, remove the thermal desorption adsorption tube containing gaseous matter from the trap, seal both ends, and it is ready for testing.

[0030] (3) Thermal desorption GC-MS analysis 1 μL of 100 μg / mL n-heptadecane internal standard solution was precisely added to both the filter thermal desorption tube and the thermal desorption adsorption tube. The thermal desorption tube and thermal desorption adsorption tube were then sequentially transferred to the thermal desorption chamber for thermal desorption, cold trap collection, and GC-MS analysis. Specific analytical conditions were as follows: Thermal desorption conditions: The sample was introduced using solvent venting mode. The temperature program was as follows: initial temperature 40℃, heating at a rate of 200℃ / min, heating to 280℃ and holding for 9 minutes; transfer line temperature 280℃; cold trap temperature -100℃, heated to 280℃ at a rate of 12℃ / s and held for 3 minutes.

[0031] GC-MS conditions: DB-5 capillary column (60m × 0.25mm, 0.25μm), constant flow mode, flow rate 1.0 mL∙min -1 The carrier gas was high-purity helium; the injection port temperature was 300 °C; the temperature program was: initial temperature 40 °C, hold for 3 min; then increase at a rate of 2 °C·min. -1 Heat to 260℃ and hold for 10 min; EI ionization mode, ion source temperature 230℃, ionization energy 70 eV; full scan and ion monitoring mode; transfer line temperature 280℃; quadrupole temperature 150℃, solvent delay 9 min.

[0032] (4) Method Evaluation Ten typical flue gas components were selected and confirmed using standard substances. A series of standard solutions with concentrations of 0.1 μg / mL, 0.2 μg / mL, 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 5 μg / mL, and 10 μg / mL were prepared using the ten standards, each containing a 100 μg / mL heptadecane internal standard. The series of standard solutions were sequentially added to empty thermal desorption tubes for thermal desorption GC-MS analysis, yielding standard curves for each component. 1 μg / mL standard solutions were added to thermal desorption filters and thermal desorption adsorption tubes, respectively, for recovery and precision (n=6) testing. The results are shown in Table 1, indicating that the method exhibits good recovery and precision.

[0033] Table 1. Quantitative linear parameters, recoveries, and precision of 10 typical flue gas components. Example 3

[0034] This embodiment uses the GC-MS method for capturing and directly thermally desorbing the particulate phase components of cigarette smoke from a single puff, as described in Example 2, to analyze the particulate phase components of the third puff of a certain brand of cigarette sample.

[0035] The GC-MS full scan images of the gaseous and particulate matter in a single puff of this brand of cigarettes are as follows: Figure 2 As shown, there are many chromatographic peaks in the chromatogram. The retention time of gas phase components is mainly concentrated in the first part of the chromatogram (RT < 30 min), while the retention time of particulate components is relatively evenly distributed throughout the chromatogram, with obvious chromatographic peaks in the range of RT 12 min to 80 min.

[0036] The GC-MS full scans of the combined thermal desorption filter and thermal desorption adsorption tube were used to qualitatively identify the components of the smoke based on mass spectrum retrieval and retention index. A total of 124 compounds were identified in the gas-particle phase of the selected cigarette sample's single-puff smoke. The qualitative results and quantitative ions for some smoke components (1-50#) are shown in Table 2. Based on this, standard substances were used to confirm the qualitative identification of important target components.

[0037] Based on the quantitative ion analysis of each component, GC-MS analysis of the gas and particulate phase components of a single flue gas was performed using selected ion mode (SIM) to obtain SIM maps of the gas and particulate phase components. Figure 3 The peak areas of each component in the gas phase and the particulate phase were compared. Semi-quantitative analysis was performed using n-heptadecane as an internal standard to obtain the distribution data of flue gas components in the gas phase and particulate phase of the third flue gas and the ratio of gas phase to particulate phase (Table 3).

[0038] Table 2. Qualitative results of some smoke components in cigarette samples Table 3. Semi-quantitative results of the main smoke components and particle ratio of the third puff of cigarette smoke.

Claims

1. A method for capturing and directly thermally desorbing particulate phase components of cigarette smoke using GC-MS, characterized in that, Includes the following steps: (1) Construct a flue gas gas-particulate phase series online capture unit. The online capture unit includes a flue gas collector and a thermal desorption adsorption tube connected in sequence along the flue gas flow direction. The flue gas collector is provided with a detachable thermal desorption filter for trapping particulate matter. The thermal desorption adsorption tube is provided with an adsorbent for capturing gas phase components passing through the thermal desorption filter. (2) Determine the preset position of the combustion line on the cigarette, connect the cigarette to the gas particle phase series capture unit and ignite it to make it smolder. When the combustion line reaches the preset position, trigger the smoking machine to suck the cigarette once, so that the smoke generated by this sucking passes through the thermal desorption filter and the thermal desorption adsorption tube in sequence, so that the particulate matter is intercepted by the thermal desorption filter and the gas phase components passing through the thermal desorption filter are captured by the adsorbent. (3) After completing the first suction, take out the thermal desorption filter, roll it up and put it into the thermal desorption tube to form a filter thermal desorption tube, wherein the maximum outer diameter of the rolled thermal desorption filter is smaller than the inner diameter of the thermal desorption tube. (4) Add internal standard to the thermal desorption tube of the filter and the thermal desorption adsorption tube containing gas phase components respectively, and perform determination under the same thermal desorption conditions, cold trap trapping conditions and GC-MS analysis conditions without solvent extraction of the thermal desorption filter and the adsorbent. (5) Based on the measurement results of the filter thermal desorption tube and the thermal desorption adsorption tube, obtain the single-particle phase distribution data of the target component in the smoke in this sampling.

2. The method according to claim 1, characterized in that, The thermal desorption filter is a glass fiber filter or a quartz fiber filter.

3. The method according to claim 1 or 2, characterized in that, The inner diameter of the thermal desorption tube is 4-8 mm, and the outer diameter of the thermal desorption filter is 15 mm. The thermal desorption filter is made by punching or cutting a Cambridge filter with an outer diameter of 44 mm.

4. The method according to claim 1, characterized in that, The adsorbent in the thermal desorption adsorption tube includes at least two adsorbent materials selected from styrene / divinylbenzene adsorbent and methyl methacrylate / divinylbenzene adsorbent.

5. The method according to claim 1, characterized in that, The preset position of the combustion line is determined by drawing a line on the cigarette paper; the single puff adopts the ISO puff mode, the puff waveform is bell-shaped, the puff volume is 35 mL, and the puff duration is 2 s.

6. The method according to claim 1, characterized in that, Before use, the thermal desorption adsorption tube is aged at 290°C for 120 min under high-purity nitrogen protection; after the first suction is completed, both ends of the filter thermal desorption tube and the thermal desorption adsorption tube are sealed respectively.

7. The method according to claim 1, characterized in that, Add 1 μL of n-heptadecane internal standard solution with a concentration of 100 μg / mL to the filter thermal desorption tube and the thermal desorption adsorption tube, respectively. The solvent of the internal standard solution is methanol or n-hexane.

8. The method according to claim 1, characterized in that, The thermal desorption conditions are: initial temperature 40℃, temperature increased to 280℃ at 200℃ / min and held for 9 min, and transmission line temperature 280℃; the cold trap trap conditions are: cold trap temperature -100℃, temperature increased to 280℃ at 12℃ / s and held for 3 min.

9. The method according to claim 1, characterized in that, The GC-MS analysis was performed using a DB-5 capillary column with helium as the carrier gas at a flow rate of 1.0 mL / min. The initial column temperature was 40℃ and held for 3 min, then increased to 260℃ at a rate of 2℃ / min and held for 10 min. The mass spectrometry was performed using EI ionization with an ionization energy of 70 eV and an ion source temperature of 230℃. Detection was performed using full scan mode and / or selected ion monitoring mode.

10. The method according to claim 1, characterized in that, The target components were preliminarily identified using mass spectrometry retrieval and retention index, and then confirmed using standard substances. Quantification was performed using an internal standard method with a series of standard solutions.

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