Detector
The microfluidic chip detector addresses home health testing challenges by integrating a substrate with mixing chambers and reagent supply, enabling rapid and accurate immunoassays with reliable results and data management.
Patent Information
- Application Number
- CN202420229468.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-30
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-01-30
AI Technical Summary
Current home health testing devices face challenges such as requiring external power and water sources, high cost, complex operation, inaccurate sample addition, lack of flexibility, and unreliable data due to the use of simple photoelectric sensors, making them unsuitable for widespread home use.
A microfluidic chip detector integrating a substrate with mixing chambers, reagent pack placement area, and liquid supply mechanism, featuring a spike for reagent piercing, injection pump, waste liquid collection, and a magnet for immune reaction adsorption, enabling rapid and accurate home immunoassays without professional training.
Facilitates efficient and rapid home immunoassays with precise sample addition and detection, providing reliable results through integration of microfluidic chip components, and supports health data management via smartphone apps.
Smart Images
Figure CN222197002U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidic instant detection, in particular to a microfluidic chip detector capable of improving integration. Background Art
[0002] With the development of science and technology, the demand for home health testing has increased and expanded, making POCT testing one of the main development directions in the testing field in recent years. POCT refers to a testing method that uses on-site sampling, on-site testing results, and the use of portable and compact analytical instruments and supporting reagents to quickly obtain the target test object.
[0003] At present, home detection instruments have not yet appeared significantly in the public eye. There are similar rapid biochemical detection devices mainly in the laboratories or inspection departments of hospitals, but these detectors have many problems: 1. Some biochemical detection instruments still require external power and water sources, which limits the application environment; 2. At present, miniaturized detection instruments still have the problem of high cost and are difficult to enter the home; 3. Since the test-related reagents need to be refrigerated, operations such as sampling require more complex equipment and professional skills, it is difficult for everyone to self-check at home; 4. Although the current test paper self-test products meet certain requirements, and some products have also entered the home (such as pregnancy test sticks), there are still problems such as inaccurate sample addition, difficulty in quantitative and multiple detection, and incorrect data reading; 5. The current home detection devices lack flexibility, and there are problems of repeated development and waste of resources; 6. Some home or personal detection devices mostly use simple photoelectric sensors to collect data, and then analyze health signs based on big data modeling, rather than directly collecting samples to detect biochemical reactions. There are inaccurate data and unreasonable test results, leading to unreliable consequences.
[0004] Therefore, the market needs a disposable detection device that is low-cost, quick and simple, can achieve precise control of the sample addition and detection process, is safe and reliable, and can be used without professional training. The present invention solves such problems.
[0005] In addition, microfluidic chip is a new technology developed on the basis of capillary electrophoresis. It integrates different functional units such as micro-channels, micro-reactors, and micro-detectors through micro-machining, and miniaturizes a biochemical reaction to a chip of a few square centimeters. In recent years, microfluidic chip technology has rapidly penetrated into the biomedical field, showing broad application prospects. With the development of technology, this technology has become an important platform for medical testing technology in the field of instant diagnosis. Utility Model Content
[0006] The technical problem to be solved by the utility model is to provide a detector for rapid home immune detection based on a microfluidic chip in response to the above-mentioned current technical status.
[0007] The technical solution adopted by the utility model to solve the above technical problems is: the detector includes an instrument body, which is characterized in that: the instrument body includes a shell, and the shell is provided with:
[0008] The reaction area of the microfluidic chip includes a substrate and a mixing chamber arranged on the substrate, wherein at least two mixing chambers are arranged at intervals, each mixing chamber is connected to a reaction chamber downstream, each mixing chamber is provided with an inlet for liquid inlet upstream, and an outlet for liquid outlet is provided on the reaction chamber located at the most downstream; correspondingly, a first socket for detachably inserting the substrate is provided on the housing;
[0009] The reagent pack placement area is located on the substrate, including a base plate and reagent slots arranged on the base plate for placing reagent packs to be reacted, each reagent slot is connected through a connecting channel, and the connecting channel is fluidically connected with the inlet on the base plate; correspondingly, a second socket for detachably inserting the base plate is provided on the housing; and
[0010] The liquid supply mechanism is located on the substrate and includes a spike that can pierce a reagent package containing a reaction liquid, an actuating member that can drive the spike to pierce the reagent packages one by one, and a driving member that drives the actuating member to actuate.
[0011] In order to facilitate the actuation of the actuator, preferably, the actuator includes a disc-shaped disc, a protrusion arranged at the periphery of the disc and extending downward toward the base plate, and the center of the disc is connected to the power output shaft of the driving member. The disc is driven to rotate by the rotation of the driving member such as a motor, so that the protrusions cooperate with the spikes one by one to pierce the reagent pack.
[0012] In order to facilitate the coordination between the spike and the protrusion, from the perspective of the simplicity of the structural setting of the spike, it is preferred that the spike is confined in a vertically arranged limiting pipe, each limiting pipe is connected to its corresponding reagent tank, and the spike includes a head that cooperates with the protrusion of the actuator and a sharp tail for piercing the reagent package, and the limiting pipe is provided with an upper opening for the head to be exposed upward, a lower opening for the sharp tail to be exposed downward, and an elastic member sleeved in the limiting pipe, and the elastic member makes the sharp tail always have a tendency to retract in the limiting pipe.
[0013] In order to facilitate the process of extending the spike tail only when piercing the reaction package and retracting when not piercing the reaction package, from the perspective of structural design, preferably, each of the limiting pipes is integrated on a limiting plate attached to the reaction area of the microfluidic chip, and the limiting plate is provided with a flow port connected to the lower opening and the reagent tank. When the raised portion of the actuator presses on the head of the spike, the spike tail can pierce the reagent package so that the reaction liquid can pierce each reagent package along the lower opening and the flow port.
[0014] Furthermore, an injection pump drive area is also provided in the shell, and the injection pump drive area includes a cavity of the injection pump, a water inlet pipe arranged on the cavity and connected to the outlet of the microfluidic chip reaction area, and a water outlet pipe arranged on the cavity. The liquid is discharged from the water outlet pipe through the reciprocating motion of the piston in the injection pump.
[0015] In order to facilitate the cleaning of the waste liquid collection chamber, preferably, a waste liquid collection area connected to the water outlet pipe is also provided in the shell, and the waste liquid collection area includes a waste liquid collection chamber. The shell is provided with a first opening corresponding to the waste liquid collection area, and the first opening can allow the waste liquid collection chamber of the waste liquid collection area to be removably inserted. Correspondingly, a handle exposed on the first opening is provided on the waste liquid collection chamber.
[0016] In order to improve the reaction speed and reaction accuracy in the reaction area of the microfluidic chip, preferably, a micro-vibrator capable of enhancing mixing and a photoelectric detector and a photoelectric receiver capable of detecting reaction concentration are also provided in the shell on the reaction area of the microfluidic chip.
[0017] Furthermore, a magnet is provided at the bottom of the reaction chamber, which can adsorb the conjugate produced by the immune reaction into the reaction chamber. Generally, in immune reactions, reagent balls having capture reagent balls and labeling reagent balls are often used, wherein the capture reagent balls have magnetic beads coated with antibodies, antigens or secondary antibodies, and the labeling reagent balls are fluorescent microspheres coated with antibodies, antigens or secondary antibodies; the sample enters the mixing chamber to dissolve the reagent balls to obtain a mixed sample, and the mixed sample enters the reaction chamber to generate an immune reaction. At this time, the magnet at the bottom of the reaction chamber adsorbs the conjugate of magnetic beads coated with antibodies, antigens or secondary antibodies produced by the immune reaction into the reaction chamber, and the reaction chamber only contains the adsorbed magnetic bead conjugate.
[0018] Furthermore, the housing is also provided with buttons that can control the microfluidic chip reaction area, the syringe pump drive area, and the waste liquid collection area. By integrating the microfluidic chip reaction area, the syringe pump drive area, the waste liquid collection area, and the buttons that control each area in the detection instrument, rapid home immune detection based on the microfluidic chip can be conveniently performed, which is one of the important technical means to achieve home health management in the future. It can also be associated with a smartphone APP account to achieve the storage of health data, real-time dynamic viewing of health data, establishment of personal health records, push of post-management intervention plans, and recommended diet and exercise plans.
[0019] In order to avoid placing the reagent pack and the substrate for the microfluidic chip in two steps, from the perspective of optimizing the integration of the operation steps, preferably, the reagent tank in the reagent pack placement area is integrated on the substrate in the microfluidic chip reaction area, and the inlet and the reagent tank for liquid inlet are respectively arranged on the upper and lower surfaces of the substrate. Integrating the substrate for placing the reagent pack and the substrate for placing the microfluidic chip together can facilitate users to replace the microfluidic chip and the reagent pack at the same time.
[0020] Compared with the prior art, the advantages of the utility model are: by integrating the microfluidic chip reaction area, the reagent package placement area and the liquid supply mechanism in the detector, rapid home immunoassay based on the microfluidic chip can be conveniently performed; in addition, since the microfluidic chip is provided with an inlet for liquid inlet upstream of the mixing chamber, the number of the inlet can be adaptively increased according to the number of samples to be reacted, which can increase the sample addition speed; each mixing chamber is provided with a liquid inlet upstream, and samples can be added to the corresponding inlets according to the different reaction orders to perform multi-stage reactions, ultimately achieving efficient and rapid fluid mixing and reaction. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 This is a schematic diagram of the structure of the detector in Example 1 of the utility model;
[0022] Figure 2 It is a cross-sectional view of the detector in Example 1 of the utility model;
[0023] Figure 3 This is a schematic diagram of the local structure of the detector in Example 1 of the utility model;
[0024] Figure 4 This is a schematic diagram of the structure of the microfluidic chip in Example 1 of the utility model;
[0025] Figure 5 This is a schematic diagram of the structure of the detector in Example 2 of the utility model;
[0026] Figure 6 It is a cross-sectional view of the detector in Example 2 of the utility model;
[0027] Figure 7 This is a schematic diagram of the local structure of the detector in Example 2 of the utility model;
[0028] Figure 8 This is a schematic diagram of the structure of the microfluidic chip in Example 2 of the utility model; DETAILED DESCRIPTION
[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0030] Example 1
[0031] like Figures 1 to 4 The detector of this embodiment includes an instrument body 1, which includes a shell 11, and a microfluidic chip reaction area 2, a reagent pack placement area 3 and a liquid supply mechanism 4 are arranged in the shell 11, wherein the microfluidic chip reaction area 2 includes a substrate 21 and a mixing chamber 22 arranged on the substrate 21, and at least two mixing chambers 22 are arranged at intervals, and each mixing chamber 22 is connected to a reaction chamber 23 downstream, and each mixing chamber 22 is provided with an inlet 24 for liquid inlet upstream, and an outlet 25 for liquid outlet is provided on the reaction chamber 23 located at the most downstream; correspondingly, a first socket 12 for detachably inserting the substrate 21 is provided on the shell 11, and the reagent pack placement area 3 is located on the substrate 21, including a base plate 31 and a reagent tank 32 arranged on the base plate 31 for placing the reagent pack to be reacted, and each reagent tank 32 is connected through a connecting channel 33, and the connecting channel 33 is fluidically connected with the inlet 24 on the substrate 21; correspondingly A second socket 13 is provided on the shell 11 for the substrate 31 to be removably inserted, and the liquid supply mechanism 4 located on the substrate 31 includes a spike 41 that can pierce a reagent package containing a reaction liquid, an actuator 42 that can drive the spike 41 to pierce the reagent packages one by one, and a driving member 43 that drives the actuator 42 to actuate. By integrating the microfluidic chip reaction area 2, the reagent package placement area 3 and the liquid supply mechanism 4 in the detector, it is convenient to perform rapid home immunoassay based on the microfluidic chip. In addition, since the microfluidic chip has an inlet 24 for liquid inlet upstream of the mixing chamber 22, the number of the inlet 24 can be adaptively increased according to the number of samples to be reacted, which can increase the sample addition speed; each mixing chamber 22 is provided with a liquid inlet 24 upstream, and samples can be added to the corresponding inlet 24 according to the different reaction orders to perform multi-stage reactions, ultimately achieving efficient and rapid fluid mixing and reaction.
[0032] Specifically, in order to facilitate the actuation of the actuator 42, the actuator 42 of this embodiment includes a disc 421 in the shape of a disc, a protrusion 422 arranged at the periphery of the disc 421 and extending downward toward the substrate 31, and the center of the disc 421 is connected to the power output shaft of the driving member 43. The rotation of the driving member 43, such as a motor, drives the rotation of the disc 421, so that the protrusions 422 are matched with the spikes 41 one by one to pierce the reagent pack. In order to facilitate the matching of the spike 41 and the protrusion 422, from the perspective of the simple structural setting of the spike 41, the spike 41 is confined in the vertically arranged limiting pipe 5, each limiting pipe 5 is connected to its own corresponding reagent tank 32, and the spike 41 includes a head 41a matched with the protrusion 422 of the actuator 42 and a spike tail 41b for piercing the reagent package, and the limiting pipe 5 is provided with an upper opening 51 for the head 41a to be exposed upward, a lower opening 52 for the spike tail 41b to be exposed downward, and an elastic member 53 sleeved in the limiting pipe 5, and the elastic member 53 makes the spike tail 41b always have There is a tendency to retract into the limiting pipe 5. The elastic member 53 here can be a compression spring, the fixed end of which is connected to the limiting pipe 5 and the free end is connected to the head 41a of the spike 41. When the protrusion 422 on the disk 421 of the actuator 42 presses the head 41a, it can drive the spike tail 41b to move downward to puncture the reaction package. When the protrusion 422 on the disk 421 of the actuator 42 leaves the head 41a, the spike tail 41b can be retracted into the limiting pipe 5 again under the restoring action of the compression spring. In this way, the spike tail 41b can be extended only when the reaction package is punctured and retracted when the reaction package is not punctured. From the perspective of convenient processing, each limiting pipe 5 can be integrated on a limiting plate 60 attached to the reaction area 2 of the microfluidic chip. The limiting plate 60 is provided with a flow port 601 connected to the lower opening 52 and the reagent tank 32. When the protrusion 422 of the actuator 42 presses on the head 41a of the spike 41, the tail 41b of the spike can pierce the reagent package so that the reaction liquid can move down along the lower opening 52 and the flow port 601, for example, 4 to 5 mm, thereby piercing each reagent package.
[0033] In addition, an injection pump driving area 6 is also provided in the shell 11, and the injection pump driving area 6 includes a cavity 61 of the injection pump, a water inlet pipe 62 arranged on the cavity 61 and connected to the outlet 25 of the microfluidic chip reaction area 2, and a water outlet pipe 63 arranged on the cavity 61. The liquid is discharged from the water outlet pipe 63 through the reciprocating motion of the piston in the injection pump.
[0034] In order to facilitate the cleaning of the waste liquid collection chamber 71, a waste liquid collection area 7 connected to the outlet pipe 63 is also provided in the shell 11. The waste liquid collection area 7 includes a waste liquid collection chamber 71. The shell 11 is provided with a first opening 14 corresponding to the waste liquid collection area 7. The first opening 14 can be removably inserted into the waste liquid collection chamber 71 of the waste liquid collection area 7. Correspondingly, a handle 72 exposed on the first opening 14 is provided on the waste liquid collection chamber 71. A micro vibrator 8 that can enhance mixing and a photoelectric detector 9 and a photoelectric receiver 10 that can detect the reaction concentration are also provided on the microfluidic chip reaction area 2 in the shell 11 to improve the reaction speed and reaction accuracy in the microfluidic chip reaction area 2. At the same time, a magnet 20 is provided at the bottom of the reaction chamber 23, and the magnet 20 can adsorb the conjugate produced by the immune reaction in the reaction chamber 23. Generally, in immune reactions, reagent balls with capture reagent balls and labeling reagent balls are often used, wherein the capture reagent balls have magnetic beads coated with antibodies, antigens or secondary antibodies, and the labeling reagent balls are fluorescent microspheres coated with antibodies, antigens or secondary antibodies; the sample enters the mixing chamber 22 to dissolve the reagent balls to obtain a mixed sample, and the mixed sample enters the reaction chamber 23 to cause an immune reaction. At this time, the magnet 20 located at the bottom of the reaction chamber 23 adsorbs the magnetic bead conjugate coated with antibodies, antigens or secondary antibodies produced by the immune reaction into the reaction chamber 23, and the reaction chamber 23 only contains the adsorbed magnetic bead conjugate. Finally, the housing 11 is also provided with a button 30 that can control the above-mentioned microfluidic chip reaction area 2, the injection pump drive area 6 and the waste liquid collection area 7. By integrating the microfluidic chip reaction area 2, the injection pump drive area 6, the waste liquid collection area 7 and the buttons 30 for controlling each area in the detection instrument, it is possible to conveniently perform rapid home immune testing based on the microfluidic chip, and it can also be linked to a smartphone APP account to realize the storage of health data, real-time dynamic viewing of health data, establishment of personal health records, push of post-management intervention plans, and recommended diet and exercise plans.
[0035] Taking the use of a microfluidic detector to detect human saliva as an example, the specific operations are as follows:
[0036] ①. Take out the disposable microfluidic chip and tear open the outer packaging;
[0037] ②. Align the top of the chip with the bottom of the tongue in the mouth, absorb a small amount of liquid, and cover it with the lid;
[0038] ③. After the chip is aligned, it is inserted into the corresponding chip slot area in the housing 11 through the second socket 13, and the substrate 31 for placing the reagent pack is inserted into the reagent pack placement area 3 in the housing 11 through the first socket 12;
[0039] ④. Turn on the detector, and the LCD screen will display the initial page;
[0040] ⑤. When you hear the beep, turn on the detection switch;
[0041] ⑥. Wait until you see the yellow light on, indicating that the test is in progress, which takes about 10 minutes;
[0042] ⑦. Wait until the green light comes on, indicating that the test is finished, and the test result and the remaining reagent amount will be shown on the display screen;
[0043] ⑧. The test data is directly transmitted to the APP on the user's mobile phone through the bound account for storage;
[0044] ⑨. After the test is completed, the chip and the substrate 31 on which the reagent pack is placed are taken out and thrown away;
[0045] ⑩. Turn off the switch, the reagent pack and disposable test chip will be packaged together as consumables, and the monitoring data will be tracked and analyzed in real time in the APP, and your latest physical health status will be pushed in real time.
[0046] Example 2 Detection process of reagent package combined with microfluidic chip
[0047] ① Once the chip card slot is inserted, the spike tail 41b of the spike object 41 punctures the No. 1 reagent pack under the actuation of the actuating member 42;
[0048] ② The reaction liquid can flow into the liquid inlet 24 of the substrate 21 along the lower opening 52 of the limiting pipe 5 and the flow port of the limiting plate 6 to contact with the sample;
[0049] ③. When you hear the beep, press the detection switch
[0050] ④. Trigger the micro vibrator 8 and vibrate and incubate for 5 minutes;
[0051] ⑤. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;
[0052] ⑥. Puncture the No. 2 reagent pack and drain all the liquid inside the chip to the waste liquid collection area 7;
[0053] ⑦. Remove the permanent magnet 20 and push in the micro vibrator 8;
[0054] ⑧. Puncture the No. 3 reagent pack and incubate for 5 minutes;
[0055] ⑨. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;
[0056] ⑩. After puncturing reagent package No. 4, push all the liquids into reaction chamber 23. Reaction chamber 23 detects values of different absorbances and displays the data on the LCD. Simultaneously, your test data results will pop up on the mobile phone APP.
[0057] Example 3
[0058] The structure is basically the same as that of Example 1, with the only difference being that the substrate 21 for placing the reagent pack and the microfluidic chip is integrated together, which can facilitate the user to replace the microfluidic chip and the reagent pack at the same time, and the reagent tank 32 of the reagent pack placement area 3 is integrated on the substrate 21 in the microfluidic chip reaction area 2, and the inlet 24 for liquid inlet and the reagent tank 32 are respectively arranged on the upper and lower surfaces of the substrate 21, such as Figures 5 to 8 shown.
Claims
1. A detector, comprising an instrument body (1), characterized in that: The instrument body (1) comprises a housing (11), and the housing (11) is provided with: A microfluidic chip reaction zone (2) comprises a substrate (21) and a mixing chamber (22) arranged on the substrate (21), wherein at least two mixing chambers (22) are arranged at intervals, each mixing chamber (22) is connected to a reaction chamber (23) downstream, each mixing chamber (22) is provided with an inlet (24) for liquid inlet upstream, and an outlet (25) for liquid outlet is provided on the reaction chamber (23) located at the most downstream; correspondingly, a first socket (12) is provided on the housing (11) for the substrate (21) to be removably inserted; The reagent pack placement area (3) is located on the substrate (21), and includes a base plate (31) and reagent slots (32) arranged on the base plate (31) for placing reagent packs to be reacted, and each reagent slot (32) is connected through a connecting channel (33), and the connecting channel (33) is fluidically connected to an inlet (24) on the substrate (21); correspondingly, a second socket (13) is provided on the housing (11) for the base plate (31) to be detachably inserted; and The liquid supply mechanism (4) is located on the substrate (31) and comprises a spike (41) capable of piercing a reagent pack containing a reaction liquid, an actuating member (42) capable of driving the spike (41) to pierce the reagent packs one by one, and a driving member (43) driving the actuating member (42) to actuate.
2. The detector according to claim 1, characterized in that: The actuating member (42) comprises a disk body (421) in the shape of a disk (421), a protrusion (422) arranged on the periphery of the disk body (421) and extending downward toward the base plate (31), and the center of the disk body (421) is connected to the power output shaft of the driving member (43).
3. The detector according to claim 2, characterized in that: The spike (41) is confined in a vertically arranged limiting pipe (5), each limiting pipe (5) is connected to its corresponding reagent tank (32), and the spike (41) includes a head (41a) that cooperates with the protrusion (422) of the actuator (42) and a spike tail (41b) for piercing the reagent package, and the limiting pipe (5) is provided with an upper opening (51) for the head (41a) to be exposed upward, a lower opening (52) for the spike tail (41b) to be exposed downward, and an elastic member (53) sleeved in the limiting pipe (5), and the elastic member (53) makes the spike tail (41b) always have a tendency to retract in the limiting pipe (5).
4. The detector according to claim 3, characterized in that: Each of the limiting pipes (5) is integrated on a limiting plate (60) attached to the reaction area (2) of the microfluidic chip. The limiting plate (60) is provided with a flow port (601) connected to the lower opening (52) and the reagent tank (32). When the protrusion (422) of the actuator (42) presses the head (41a) of the spike (41), the tail (41b) of the spike can pierce the reagent pack so that the reaction liquid can pierce each reagent pack along the lower opening (52) and the flow port (601).
5. The detector according to any one of claims 1 to 4, characterized in that: The housing (11) is also provided with a syringe pump drive area (6), the syringe pump drive area (6) comprising a syringe pump cavity (61), a water inlet pipe (62) provided on the cavity (61) and connected to an outlet (25) of a microfluidic chip reaction area (2), and a water outlet pipe (63) provided on the cavity (61), wherein the liquid is discharged from the water outlet pipe (63) by the reciprocating motion of the piston in the syringe pump.
6. The detector according to claim 5, characterized in that: The shell (11) is also provided with a waste liquid collection area (7) connected to the water outlet pipe (63), the waste liquid collection area (7) includes a waste liquid collection chamber (71), the shell (11) is provided with a first opening (14) corresponding to the waste liquid collection area (7), the first opening (14) can be used for the waste liquid collection chamber (71) of the waste liquid collection area (7) to be removably inserted, and correspondingly, the waste liquid collection chamber (71) is provided with a handle (72) exposed on the first opening (14).
7. The detector according to claim 6, characterized in that: A micro vibrator (8) capable of enhancing mixing, as well as a photoelectric detector (9) and a photoelectric receiver (10) capable of detecting reaction concentration are also arranged in the housing (11) on the microfluidic chip reaction area (2).
8. The detector according to claim 7, characterized in that: A magnet (20) is disposed at the bottom of the reaction chamber (23), and the magnet (20) can adsorb the conjugate produced by the immune reaction into the reaction chamber (23).
9. The detector according to any one of claims 6 to 8, characterized in that: The housing (11) is also provided with buttons (30) capable of controlling the microfluidic chip reaction area (2), the injection pump drive area (6) and the waste liquid collection area (7).
10. The detector according to claim 1, characterized in that: The reagent tank (32) of the reagent pack placement area (3) is integrated on the substrate (21) in the microfluidic chip reaction area (2), and the inlet (24) for liquid inlet and the reagent tank (32) are respectively arranged on the upper and lower surfaces of the substrate (21).