Western blot membrane transfer box
By introducing cooling tanks and multiple transfer membrane clamps into the western blot transfer box, the problem of the equipment not being able to maintain low temperature and low efficiency is solved, and the cooling of proteins and high-efficiency transfer treatment is achieved.
Patent Information
- Application Number
- CN202421109319.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-20
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-05-20
AI Technical Summary
Existing Western blot membrane transfer equipment cannot maintain low temperatures, resulting in thermal damage to proteins. The experimental results are inaccurate. At the same time, only two membrane transfer clips can be processed at one time, which is inefficient.
A western-blot transfer box is designed, with a cooling tank and a cooling medium inside. The gel plate in the transfer tank is cooled and cooled by the cooling medium in the cooling tank, and multiple transfer films are provided in the transfer tank. The clip position supports the simultaneous rotation of multiple gel plates.
It effectively prevents proteins from being damaged by heat, ensures the accuracy of experimental results, and improves the efficiency of membrane transfer treatment, and can process multiple gel plates at the same time.
Smart Images
Figure CN222203315U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to protein blotting transfer equipment, in particular to a protein blotting transfer box. Background Art
[0002] Immunoblotting, also known as protein blotting, is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody.
[0003] The method is to separate the mixed antigen sample by unidirectional or bidirectional electrophoresis on a gel plate, then place an immobilized matrix membrane against the gel, and under the action of the natural adsorption force of the blotting paper, the electric field force or other external forces, transfer the single antigen component in the gel to the blotting paper and solidify it to complete the membrane transfer step, and finally use immune covering fluid technology such as immune isotope probes or immune enzyme probes to detect and analyze the antigen immobilized matrix membrane.
[0004] In the prior art, the equipment used for membrane transfer usually does not have the ability to maintain low temperatures, which can easily cause heat damage to proteins and lead to inaccurate experimental results. At the same time, most of the existing membrane transfer equipment on the market can only place two transfer clips at a time for membrane transfer.
[0005] Therefore, it is necessary to design a new type of protein blotting transfer box to solve the above-mentioned technical problems. Utility Model Content
[0006] The technical problem to be solved by the utility model is to provide a protein blot transfer box, which can solve the problem of inaccurate experimental results caused by high ambient temperature during the transfer process and perform transfer of multiple protein blots with higher efficiency.
[0007] In order to solve the above technical problems, the utility model provides a protein blotting transfer box, comprising a box body, wherein a box cavity partition is provided in the box body, the box cavity partition itself is enclosed to form a transfer groove, and a cooling groove suitable for accommodating a cooling medium is formed between the box cavity partition and the box body;
[0008] At least three film transfer clamp positions are formed in the film transfer groove, and a pair of oppositely arranged plate surfaces of the box cavity partition are respectively connected to the positive electrode and the negative electrode of the power supply.
[0009] Furthermore, a plurality of pairs of limiting columns are provided in the transfer groove, and the transfer clamps are formed between adjacent pairs of the limiting columns.
[0010] Furthermore, a portion of the box cavity partition close to the bottom wall of the box body is provided with a lower connecting port, so that the film transfer groove can be connected with the cooling groove via the upper connecting port.
[0011] Furthermore, a portion of the box cavity partition away from the bottom wall of the box body is provided with an upper connecting port, so that the film transfer groove can be connected with the cooling groove via the lower connecting port.
[0012] Furthermore, a cooling groove partition is provided in the cooling groove, the cooling groove partition is connected to the bottom wall of the box body, and the cooling groove partition itself can be enclosed and closed to form an inner cooling groove between the cooling groove partition and the box cavity partition, and an outer cooling groove is formed between the cooling groove partition and the side wall of the box body, and the transfer groove is connected to the inner cooling groove.
[0013] Furthermore, the inner cooling groove is suitable for inserting a cooling block.
[0014] Furthermore, the cooling block includes an abutting frame and a plurality of heat exchange tubes connected to the abutting frame and perpendicular to the abutting frame, the abutting frame can abut between the cooling slot partition and the box cavity partition, and the abutting frame and the heat exchange tube are both provided with a medium accommodating cavity suitable for accommodating a cooling medium.
[0015] Furthermore, each of the heat exchange tubes is provided with a heat exchange fin.
[0016] Furthermore, it also includes a cover body, which can be buckled with the box body, and a plug-in groove is provided at a position on the cover body corresponding to the position of the transfer clamp.
[0017] Furthermore, a heat insulation layer is provided on a side of the cover body facing the film transfer tank.
[0018] Through the above technical solution, the beneficial effects of the utility model are as follows:
[0019] The utility model provides a protein blotting transfer box, comprising a box body, wherein a box cavity partition is arranged in the box body, the box cavity partition itself can be enclosed to form a transfer groove, and a cooling groove suitable for accommodating a cooling medium is formed between the box cavity partition and the box body, so that the cooling medium in the cooling groove can be used to cool down the gel plate in the transfer groove and the protein on the immobilized matrix membrane, so that the protein is not easily damaged by heat during the transfer process, thereby ensuring the effectiveness of the experiment and the accuracy of the experimental results; at least three transfer clamp positions are arranged in the transfer groove, which can transfer multiple gel plates at one time, thereby improving the efficiency of the transfer process.
[0020] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is a schematic diagram of the overall structure of the protein blotting transfer box of the utility model (wherein the cooling block is omitted);
[0022] Figure 2 It is a top view of the protein blot transfer box of the utility model;
[0023] Figure 3 The utility model is a protein blotting transfer box Figure 2 Sectional view in the AA direction;
[0024] Figure 4 It is a cross-sectional view of the cover body in the protein blotting transfer box of the utility model.
[0025] Description of Reference Numerals
[0026] 1. Box body 11. Transfer tank
[0027] 111. Transfer clamp position 112. Limiting column
[0028] 12. Inner cooling groove 13. Outer cooling groove
[0029] 2. Box cavity partition 21, lower connecting port
[0030] 22. Upper connecting port 3. Cooling tank partition
[0031] 4. Cooling block 41, abutting frame
[0032] 42. Heat exchange tube 421. Heat exchange fin
[0033] 5. Cover 51, plug-in slot
[0034] 52. Thermal insulation layer DETAILED DESCRIPTION
[0035] The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation modes described herein are only used to illustrate and explain the present invention, and the protection scope of the present invention is not limited to the specific implementation modes described below.
[0036] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "set", "provided with", and "connected" should be understood in a broad sense. For example, the connection can be a direct connection or an indirect connection through an intermediate medium, a fixed connection, a detachable connection, or an integral connection; it can be a direct connection or an indirect connection through an intermediate connection member, and it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
[0037] In the present invention, unless otherwise specified, the directional words used, such as "inside" and "outside", are defined as the inside and outside of the corresponding parts. Specifically, in the drawings provided in the present invention, the orientation or positional relationship used is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention; the orientation terms of the present invention should be understood in conjunction with the actual installation state.
[0038] like Figures 1 to 4 As shown, the utility model provides a protein blotting transfer box, including a box body 1, a box cavity partition 2 is provided in the box body 1, the box cavity partition 2 is connected to the bottom wall of the box body 1, and the box cavity partition 2 itself can be enclosed to form a transfer groove 11, and a cooling groove suitable for accommodating a cooling medium is formed between the box cavity partition 2 and the box body 1, so that the gel plate in the transfer groove 11 and the protein on the immobilized matrix membrane can be cooled by the cooling medium in the cooling groove, so that the protein is not easily damaged by heat during the transfer process, thereby ensuring the effectiveness of the experiment and the accuracy of the experimental results. In addition, at least three transfer clamp positions 111 are formed in the transfer groove 11, and a pair of relatively arranged plate surfaces of the box cavity partition 2 are respectively connected to the positive and negative electrodes of the power supply, so that multiple gel plates can be transferred at one time, so as to improve the efficiency of the transfer process.
[0039] Specifically, Figure 1 and Figure 2 As shown, a plurality of pairs of limiting columns 112 are provided in the transfer groove 11. For example, five limiting columns 112 can be provided on each of a pair of oppositely disposed plate surfaces of the box cavity partition 2. Each limiting column 112 extends along the depth direction of the transfer groove 11, so that a transfer clamp position 111 can be formed between adjacent limiting columns 112, that is, four pairs of transfer clamp positions 111 are formed on a pair of oppositely disposed plate surfaces of the box cavity partition 2, and each pair of transfer clamp positions 111 can be used to fix a gel plate.
[0040] Of course, it is understandable that three limiting columns 112 can be respectively set on a pair of oppositely arranged plate surfaces of the box cavity partition 2, and each limiting column 112 extends along the depth direction of the transfer groove 11, so that a transfer membrane clamp position 111 can be formed between adjacent pairs of limiting columns 112. In addition, a transfer membrane clamp position 111 can be formed between the limiting columns 112 and the plate surface adjacent to the plate surface where the limiting columns 112 are set, that is, four pairs of transfer membrane clamp positions 111 are formed on a pair of oppositely arranged plate surfaces of the box cavity partition 2, and each pair of transfer membrane clamp positions 111 can be used to fix a gel plate.
[0041] Furthermore, if Figure 1 and Figure 3 As shown, a lower connecting port 21 may be provided at a portion of the box cavity partition 2 close to the bottom wall of the box body 1, so that the transfer tank 11 can be connected with the cooling tank via the lower connecting port 21, and an upper connecting port 22 may be further provided at a portion of the box cavity partition 2 away from the bottom wall of the box body 1, so that the transfer tank 11 can be connected with the cooling tank via the upper connecting port 22, so that the electrophoresis liquid in the transfer tank 11 can enter the cooling tank for circulation, so as to ensure that the electrophoresis liquid in the transfer tank 11 maintains a relatively low temperature, for example, maintained at 3°C to 5°C, to ensure that the protein on the gel plate is not easily damaged due to high temperature, thereby ensuring the effectiveness of the experiment and the accuracy of the experimental results.
[0042] Furthermore, a cooling groove partition 3 is provided in the cooling groove, and the cooling groove partition 3 is connected to the bottom wall of the box body 1, and the cooling groove partition 3 itself can be enclosed and closed to form an inner cooling groove 12 between the cooling groove partition 3 and the box cavity partition 2, and an outer cooling groove 13 is formed between the cooling groove partition 3 and the side wall of the box body 1, and the transfer groove 11 is connected to the inner cooling groove 12, but the inner cooling groove 12 is not connected to the outer cooling groove 13, wherein the inner cooling groove 12 is suitable for inserting a cooling block 4, so that the electrophoresis liquid can be cooled by the cooling block 4, and the cooling block 4 is plugged into the cooling groove in a plug-in manner, which can facilitate the timely replacement of the cooling block 4, so that when a large amount of cooling is required, the cooling block 4 can be replaced in a timely manner. When transferring films in batches, it is only necessary to replace the cooling block 4 in time to maintain the low temperature state in the transfer tank 11, and the outer cooling tank 13 can accommodate a cooling medium (such as ice cubes), so that the inner cooling tank 12 can be blocked from the external environment through the outer cooling tank 13, so that the heat conducted from the outside to the protein blot transfer box can be first absorbed by the cooling medium in the outer cooling tank 13, so that the temperature of the inner cooling tank 12 can rise slowly. Of course, the outer cooling tank 13 can also be used to fill insulation material to make the speed of heat conduction to the inner cooling tank 12 slower, so that there is no need to frequently replace the cooling block 4 in the inner cooling tank 12.
[0043] It should be noted that the cooling block 4 should be made of a material that does not react with the electrophoretic liquid, such as a plastic material. The cooling block 4 can be specifically configured to include an abutting frame 41 and a plurality of heat exchange tubes 42 connected to the abutting frame 41 and perpendicular to the abutting frame 41, wherein the abutting frame 41 can be configured as a pair, one end of each heat exchange tube 42 is connected to one abutting frame 41, and the other end of each heat exchange tube 42 is connected to the other abutting frame 41, and a suitable container for accommodating the cooling medium is provided in the abutting frame 41 and the heat exchange tube 42. Medium accommodating cavity, therefore, the cooling block 4 filled with cooling medium can be placed in the refrigerator for freezing and standby use. When the cooling block 4 is needed, the cooling block 4 is taken out and inserted into the inner cooling groove 12. Specifically, the cooling block 4 should be evenly inserted in the inner cooling groove 12, and the cooling block 4 can be abutted between the cooling groove partition 3 and the box cavity partition 2 through the abutting frame 41, so that the cooling block 4 is not easy to change position with the circulation of the electrophoretic liquid, and the temperature of the electrophoretic liquid in the transfer groove 11 and the inner cooling groove 12 can be ensured to be basically consistent.
[0044] Furthermore, if Figure 3 As shown, each heat exchange tube 42 is provided with a heat exchange fin 421, so as to increase the heat exchange area of the cooling block 4 and realize rapid cooling of the electrophoretic fluid by the cooling block 4. Specifically, taking the heat exchange tube 42 in the cooling block 4 as an example in which the heat exchange tube 42 is inserted into the inner cooling groove 12 in a posture extending along the depth direction of the inner cooling groove 12, the farther the heat exchange tube 42 is from the bottom wall of the inner cooling groove 12, the larger the area of the heat exchange fin 421 is, so that the heat exchange area between the electrophoretic fluid that flows upward due to the heat and the cooling block 4 is larger, thereby realizing timely cooling of the electrophoretic fluid.
[0045] In addition, it can be understood that the protein blotting transfer box of the present application also includes a cover 5, such as Figure 1 and Figure 4 As shown, the cover body 5 can be buckled with the box body 1, and a plug-in slot 51 is provided at a position on the cover body 5 corresponding to the transfer clamp position 111 to facilitate insertion, and a heat insulation layer 52 is provided on the side of the cover body 5 facing the transfer slot 11, so that after the cover body 5 is buckled with the box body 1, the electrophoresis liquid is not easy to directly exchange heat with the external environment, thereby preventing the electrophoresis liquid from rapidly heating up.
[0046] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, and these simple modifications all belong to the protection scope of the present invention.
[0047] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.
[0048] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.
Claims
1. A protein blotting transfer cassette, characterized in that: The invention comprises a box body (1), wherein a box cavity partition (2) is arranged inside the box body (1), the box cavity partition (2) itself is enclosed to form a film transfer groove (11), and a cooling groove suitable for accommodating a cooling medium is formed between the box cavity partition (2) and the box body (1); At least three transfer clamp positions (111) are formed in the transfer groove (11), and a pair of oppositely disposed plate surfaces of the box cavity partition (2) are respectively connected to the positive electrode and the negative electrode of the power supply.
2. The protein blotting transfer cassette according to claim 1, characterized in that: A plurality of pairs of limiting columns (112) are arranged in the transfer groove (11), and the transfer clamp positions (111) are formed between adjacent pairs of the limiting columns (112).
3. The protein blotting transfer cassette according to claim 2, characterized in that: A portion of the box cavity partition (2) close to the bottom wall of the box body (1) is provided with a lower connecting port (21) so that the film transfer groove (11) can be connected with the cooling groove via the lower connecting port (21).
4. The protein blotting transfer cassette according to claim 3, characterized in that: An upper connecting port (22) is provided at a portion of the box cavity partition (2) away from the bottom wall of the box body (1) so as to enable the transfer tank (11) to be connected with the cooling tank via the upper connecting port (22).
5. The protein blot transfer cassette according to claim 3 or 4, characterized in that: A cooling groove partition (3) is provided in the cooling groove, the cooling groove partition (3) is connected to the bottom wall of the box body (1), and the cooling groove partition (3) itself can be enclosed and closed to form an inner cooling groove (12) between the cooling groove partition (3) and the box cavity partition (2), and an outer cooling groove (13) is formed between the cooling groove partition (3) and the side wall of the box body (1), and the film transfer groove (11) is connected to the inner cooling groove (12).
6. The protein blotting transfer cassette according to claim 5, characterized in that: The inner cooling groove (12) is suitable for inserting a cooling block (4).
7. The protein blotting transfer cassette according to claim 6, characterized in that: The cooling block (4) comprises an abutting frame (41) and a plurality of heat exchange tubes (42) connected to the abutting frame (41) and perpendicular to the abutting frame (41); the abutting frame (41) can abut between the cooling slot partition (3) and the box cavity partition (2); and a medium accommodating cavity suitable for accommodating a cooling medium is provided in the abutting frame (41) and the heat exchange tubes (42).
8. The protein blotting transfer cassette according to claim 7, characterized in that: Each of the heat exchange tubes (42) is provided with a heat exchange fin (421).
9. The protein blotting transfer cassette according to claim 8, characterized in that: It also comprises a cover body (5), the cover body (5) being capable of being buckled with the box body (1), and a plug-in slot (51) is provided at a position on the cover body (5) corresponding to the film transfer clamp position (111).
10. The protein blotting transfer cassette according to claim 9, characterized in that: A heat insulation layer (52) is provided on one side of the cover body (5) facing the film transfer groove (11).