Stem cell extraction and suction device

Abstract

The utility model relates to the field of stem cell extraction devices, in particular to a stem cell extraction and suction device, and aims to solve the technical problems that the conventional stem cell extraction device does not have the capability of fixing the leg bone of a rat and needs to be fixed by holding tweezers for a long time by an experimenter; according to the technical scheme, the stem cell extraction and suction device comprises an experimental platform, a culture dish, a centrifugal machine, a complete culture solution injection assembly, a fixing assembly, a lifting assembly and a sliding assembly; according to the stem cell extraction and suction device, the fixing assembly on the stem cell extraction and suction device is used for fixing the leg bone of the rat, the original mode that a worker holds tweezers with hands to fix the leg bone of the rat is replaced, one hand of an experimenter is liberated, the operation burden of the experimenter is relieved, and the operation efficiency is improved. The problems that an existing stem cell extraction device does not have the capacity of fixing the leg bone of a rat, and an experimenter needs to hold tweezers for a long time for fixing are solved.

Description

Technical Field

[0001] The utility model belongs to the field of stem cell extraction devices, and in particular relates to a stem cell extraction suction device. Background Art

[0002] Bone marrow mesenchymal stem cells are important cells that can be induced to differentiate into various cell types such as osteocytes, chondrocytes, cardiomyocytes, etc. and have the function of supporting and regulating hematopoiesis. They are often used in various scientific experiments. In these experiments, it is often necessary to extract mesenchymal stem cells from the leg bones of rats.

[0003] At present, in the existing stem cell extraction experiments, the experimenter is required to use tweezers to fix the rat leg bone with one hand, and use the other hand to continuously use culture medium to flush the rat leg bone to flush out the bone marrow in the rat leg bone. This operation process requires the experimenter to hold the tweezers to fix the rat leg bone for a long time, which is a heavy operation burden and may also cause unstable clamping operation accidents.

[0004] Therefore, in view of the problem that the above-mentioned existing stem cell extraction devices do not have the ability to fix rat leg bones, and require experimenters to hold tweezers for a long time to operate, which is a heavy operation burden, a stem cell extraction suction device is designed to fix rat leg bones instead of experimenters, thereby reducing the operation burden. Utility Model Content

[0005] In order to overcome the problem that the existing stem cell extraction device does not have the ability to fix the rat leg bones, the experimenter needs to hold the tweezers for a long time to fix them.

[0006] The technical solution of the utility model is: a stem cell extraction and suction device, including an experimental platform, a culture dish, a centrifuge, a complete culture solution injection component, a fixed component, a lifting component, and a sliding component; a culture dish is placed on the experimental platform, a centrifuge is placed on the experimental platform, a complete culture solution injection component is fixedly connected to the experimental platform, a fixed component located above the culture dish is arranged on the experimental platform, a lifting component for adjusting the height of the fixed component is arranged on the experimental platform, and a sliding component for moving the fixed component is installed on the experimental platform.

[0007] Preferably, a fixing component is added to fix the rat leg bones, instead of using handheld tweezers to fix the leg bones, thereby reducing the operating burden. The lifting component can change the height of the fixing component, the sliding component can move the fixing component, and the complete culture medium injection component can directly inject complete culture medium without repeated suction.

[0008] Preferably, the complete culture medium injection assembly includes a fixed bracket, a liquid storage bottle, and an injection tube; a fixed bracket with an insertion hole on the surface is fixedly connected to the experimental platform, a liquid storage bottle filled with complete culture medium is placed on the fixed bracket, an injection tube connected to the liquid storage bottle and provided with a valve structure is inserted into the insertion hole, the injection tube is close to the inner wall of the culture dish, the injection tube can be opened or closed by the valve, and scales are marked on the liquid storage bottle and the injection tube to facilitate observation of the amount of complete culture medium injected.

[0009] Preferably, a support frame is fixedly connected to the experimental platform, and seven storage tubes arranged from front to back are inserted into the support frame. A tweezers, a syringe, two test tubes and three liquid collection tubes are placed in the storage tubes. The storage tubes and the support frame store the objects needed for the experiment in a row, making it convenient for experimenters to take them at any time without worrying about losing or forgetting items.

[0010] Preferably, the fixing assembly includes a support plate and a clamping plate; a support plate is arranged above the experimental platform, and a clamping plate opposite to the support plate is arranged on the rear side of the support plate, and force is applied to the rat leg bones from two opposite directions through the clamping plate and the support plate, thereby achieving the effect of clamping and fixing the rat leg bones, thereby replacing the manual hand-held tweezers to fix the rat leg bones.

[0011] Preferably, the fixing assembly also includes a threaded rod, a limiting column, and a knob; a threaded rod is inserted into the support plate, the limiting column is fixedly connected to the support plate, a screw hole matching the threaded structure on the threaded rod is provided on the clamping plate, the clamping plate is sleeved on the outside of the limiting column, a knob is fixedly connected to one end of the threaded rod, and the threaded rod is rotated by turning the knob. Under the restriction of the limiting column, the clamping plate with the screw hole cannot rotate with the threaded rod, but moves back and forth on the threaded rod and the limiting column.

[0012] Preferably, the lifting assembly includes a lifting rod and a lifting ring; a lifting rod is provided on the experimental platform, and a lifting ring located on the front side of the support plate is sleeved on the lifting rod, and a fixing bolt is screwed in the lifting ring. The lifting ring drives the fixing assembly to move up and down on the lifting column, so as to achieve the effect of changing the height of the fixing assembly. After the fixing bolt is tightened, the lifting ring is slightly deformed to clamp the lifting column to fix it.

[0013] Preferably, the lifting assembly also includes a rotating shaft; the rotating shaft is fixedly connected to the front side of the support plate, and the rotating shaft is movably connected to the lifting ring. By rotating the rotating shaft, the angle of the fixed assembly and the rat leg bone fixed on the fixed assembly can be changed to make it inclined at an angle, which is convenient for flushing out the bone marrow.

[0014] Preferably, the sliding assembly includes a slide groove and a slider; a slide groove is provided on the experimental platform, and the slider is slidably connected in the slide groove, and the lifting column is fixedly connected to the slider. The position of the lifting assembly installed on the slider and the fixed assembly connected to the lifting assembly is changed by moving the slider in the slide groove to avoid obstruction caused by the fixed assembly during subsequent operations.

[0015] Beneficial effects of the utility model:

[0016] 1. By designing a stem cell extraction and suction device, the rat leg bones are fixed by using the fixing components on the stem cell extraction and suction device, instead of the original method of fixing the rat leg bones by hand-held tweezers by the staff, one hand of the experimenter is freed, and the operation burden of the experimenter is reduced. At the same time, the fixing component can be adjusted according to the bone diameter of the rat leg bones, thereby improving the versatility of the fixing component;

[0017] 2. The injection tube with a substitute valve structure can directly inject the complete culture solution into the culture dish, avoiding the user to repeatedly unscrew the lid of the storage bottle containing the culture solution and use the liquid collection tube to draw the culture solution from it. The storage bottle and the injection tube are marked with scale lines, which is convenient for the experimenter to observe the amount of complete culture solution taken out;

[0018] 3. The support frame and the container tube collect all the parts needed for the experiment together, making it convenient for experimenters to use them. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The first three-dimensional structure diagram of the stem cell extraction and suction device of the utility model is shown;

[0020] Figure 2 The second stereoscopic structure diagram of the stem cell extraction and suction device of the utility model is shown;

[0021] Figure 3 The three-dimensional structure diagram of the experimental platform of the utility model stem cell extraction and suction device is shown;

[0022] Figure 4 The figure shows a three-dimensional structure diagram of the fixing assembly of the stem cell extraction and suction device of the present invention;

[0023] Figure 5 Shown is a schematic diagram of the three-dimensional structure of the fixing component, lifting component and slider of the stem cell extraction and suction device of the present invention.

[0024] Explanation of the accompanying drawings: 1. experimental platform; 2. culture dish; 3. centrifuge; 401. fixed bracket; 402. liquid storage bottle; 403. injection tube; 5. support frame; 6. container tube; 701. support plate; 702. clamping plate; 703. threaded rod; 704. limiting column; 705. knob; 801. lifting rod; 802. lifting ring; 803. rotating shaft; 901. slide groove; 902. slider. DETAILED DESCRIPTION

[0025] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0026] See also Figure 1-2 The utility model provides an embodiment: a stem cell extraction and suction device includes an experimental platform 1, a culture dish 2, a centrifuge 3, a complete culture solution injection component, a fixed component, a lifting component, and a sliding component; a culture dish 2 is placed on the experimental platform 1, a centrifuge 3 is placed on the experimental platform 1, a complete culture solution injection component is fixedly connected to the experimental platform 1, a fixed component located above the culture dish 2 is provided on the experimental platform 1, a lifting component for adjusting the height of the fixed component is provided on the experimental platform 1, and a sliding component for moving the fixed component is installed on the experimental platform 1. The rat leg bone is fixed by adding the fixed component, instead of fixing the leg bone with hand-held tweezers, which reduces the operating burden. The lifting component can change the height of the fixed component, and the sliding component can move the fixed component. The complete culture solution injection component can directly inject complete culture solution without repeated suction.

[0027] See also Figure 1-3 In this embodiment, the complete culture solution injection assembly includes a fixed bracket 401, a liquid storage bottle 402, and an injection tube 403; the experimental platform 1 is fixedly connected to a fixed bracket 401 with an insertion hole on the surface, and a liquid storage bottle 402 filled with complete culture solution is placed on the fixed bracket 401. The insertion hole is plugged with an injection tube 403 connected to the liquid storage bottle 402 and provided with a valve structure, and the injection tube 403 is close to the inner wall of the culture dish 2. The experimental platform 1 is fixedly connected to a support frame 5, and the support frame 5 Seven storage tubes 6 are inserted in it and arranged from front to back. A tweezer, a syringe, two test tubes and three liquid collection tubes are placed in the storage tube 6. The injection tube 403 is close to the inner wall of the culture dish 2. The injection tube 403 can be opened or closed by a valve. The liquid storage bottle 402 and the injection tube 403 are marked with scales to facilitate observation of the amount of complete culture solution injected. The storage tube 6 and the support frame 5 store the objects needed for the experiment in a row, which is convenient for the experimenter to use at any time without worrying about losing or forgetting items.

[0028] See also Figure 3-5In this embodiment, the fixing assembly includes a support plate 701 and a clamping plate 702; a support plate 701 is arranged above the experimental platform 1, and a clamping plate 702 opposite to the support plate 701 is arranged at the rear side of the support plate 701, and the fixing assembly also includes a threaded rod 703, a limiting column 704, and a knob 705; a threaded rod 703 is inserted into the support plate 701, and the limiting column 704 is fixedly connected to the support plate 701, and a screw hole matching the threaded structure on the threaded rod 703 is opened on the clamping plate 702, and the clamping plate 702 is sleeved on the outer side of the limiting column 704, and the threaded rod 703 is inserted into the support plate 701, and the limiting column 704 is fixedly connected to the support plate 701, and the threaded rod 703 is inserted into ... A knob 705 is fixedly connected to one end of the rod 703, and the lifting assembly includes a lifting rod 801 and a lifting ring 802; a lifting rod 801 is provided on the experimental platform 1, and a lifting ring 802 located on the front side of the support plate 701 is sleeved on the lifting rod 801, and a fixing bolt is screwed in the lifting ring 802, and the lifting assembly also includes a rotating shaft 803; a rotating shaft 803 is fixedly connected to the front side of the support plate 701, and the rotating shaft 803 is movably connected in the lifting ring 802, and the sliding assembly includes a sliding groove 901 and a sliding block 902; a sliding groove 901 and a sliding block 902 are provided on the experimental platform 1 A slider 902 is slidably connected in the groove 901, and the lifting column is fixedly connected to the slider 902. The clamping plate 702 and the support plate 701 exert force on the rat leg bone from two opposite directions, so as to clamp and fix the rat leg bone, thereby replacing the manual hand-held tweezers to fix the rat leg bone. The knob 705 is turned to drive the threaded rod 703 to rotate. Under the restriction of the limit column 704, the clamping plate 702 with the screw hole cannot rotate with the threaded rod 703, but moves forward and backward on the threaded rod 703 and the limit column 704. 02 drives the fixed component to move up and down on the lifting column, so as to achieve the effect of changing the height of the fixed component. After tightening the fixing bolt, the lifting ring 802 is driven to slightly deform and clamp the lifting column to fix it. By rotating the rotating shaft 803, the angle of the fixed component and the rat leg bone fixed on the fixed component can be changed to make it inclined, which is convenient for flushing out the bone marrow. The position of the lifting component installed on the slider 902 and the fixed component connected to the lifting component can be changed by moving the slider 902 in the slide groove 901, so as to avoid obstruction caused by the fixed component during subsequent operations.

[0029] When working, the staff first places the sterilized stem cell extraction and suction device on the table, then takes a culture dish 2 and places it on the experimental platform 1, moves the culture dish 2 to the bottom of the injection tube 403, opens the valve, injects an appropriate amount of complete culture solution into the culture dish 2, and then removes the culture dish 2;

[0030] Take a new culture dish 2 and place it on the experimental platform 1, then take tweezers from the container tube 6, then clamp the treated rat leg bone and move it to one side close to the support plate 701, and use the other hand to turn the knob 705, the knob 705 drives the threaded rod 703 to rotate, and under the restriction of the limit column 704, the clamping plate 702 moves closer to the support plate 701 to clamp and fix the rat leg bone;

[0031] Rotate the rotating shaft 803 to make the rat leg bone present a certain inclination, then loosen the fixing bolt, and then move the lifting ring 802 downward. After moving to a suitable position, tighten the fixing bolt, and the lifting ring 802 deforms and clamps the lifting column to play a fixing role;

[0032] Take out the syringe from the container tube 6, absorb the complete culture solution in the culture dish 2, aim the syringe needle at the rat leg bone, inject the culture solution into it, so that the bone marrow inside is flushed out, thereby flushing out the bone marrow in multiple leg bones, and then take it out from the fixed component, move the sliding block 902 to the front side, and then move the fixed component away, take out the liquid collection tube from the container tube 6, repeatedly aspirate and spray out the culture solution, then take out the test tube, inject it into the test tube, put the test tube into the centrifuge 3 for centrifugation, take it out after centrifugation, use a new liquid collection tube to suck out the upper clear liquid, and then inject complete culture solution into it to make a suspension, waiting for subsequent processing.

[0033] Through the above steps, a stem cell extraction and suction device is designed, and the rat leg bones are fixed by using the fixing component on the stem cell extraction and suction device, instead of the original method of fixing the rat leg bones by the staff holding the tweezers, freeing one hand of the experimenter and reducing the experimental staff's operating burden. At the same time, the fixing component can be adjusted according to the bone diameter of the rat leg bones, thereby improving the versatility of the fixing component, so as to solve the problem that the existing stem cell extraction device does not have the ability to fix the rat leg bones and requires the experimenter to hold the tweezers for a long time for fixation.

[0034] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the purpose of the present invention.

Claims

1. A stem cell extraction and suction device, comprising an experimental platform (1), a culture dish (2), and a centrifuge (3); characterized in that: The experimental platform (1) also includes a complete culture solution injection component, a fixed component, a lifting component, and a sliding component; a culture dish (2) is placed on the experimental platform (1), a centrifuge (3) is placed on the experimental platform (1), a complete culture solution injection component is fixedly connected to the experimental platform (1), a fixed component located above the culture dish (2) is arranged on the experimental platform (1), a lifting component for adjusting the height of the fixed component is arranged on the experimental platform (1), and a sliding component for moving the fixed component is installed on the experimental platform (1).

2. A stem cell extraction and aspiration device according to claim 1, characterized in that: The complete culture solution injection assembly comprises a fixed support (401), a liquid storage bottle (402), and an injection tube (403); a fixed support (401) with an insertion hole on its surface is fixedly connected to the experimental platform (1); a liquid storage bottle (402) filled with complete culture solution is placed on the fixed support (401); an injection tube (403) connected to the liquid storage bottle (402) and provided with a valve structure is inserted into the insertion hole; and the injection tube (403) is closely attached to the inner wall of the culture dish (2).

3. A stem cell extraction and suction device according to claim 1, characterized in that: A support frame (5) is fixedly connected to the experimental platform (1), and seven storage tubes (6) arranged from front to back are inserted into the support frame (5). A pair of tweezers, a syringe, two test tubes and three liquid collection tubes are placed in the storage tubes (6).

4. A stem cell extraction and aspiration device according to claim 1, characterized in that: The fixing assembly comprises a support plate (701) and a clamping plate (702); the support plate (701) is arranged above the experimental platform (1), and a clamping plate (702) opposite to the support plate (701) is arranged at the rear side of the support plate (701).

5. A stem cell extraction and aspiration device according to claim 4, characterized in that: The fixing assembly also includes a threaded rod (703), a limiting column (704), and a knob (705); the threaded rod (703) is inserted into the support plate (701), the limiting column (704) is fixedly connected to the support plate (701), a screw hole matching the threaded structure on the threaded rod (703) is opened on the clamping plate (702), the clamping plate (702) is sleeved on the outside of the limiting column (704), and one end of the threaded rod (703) is fixedly connected to the knob (705).

6. A stem cell extraction and aspiration device according to claim 4, characterized in that: The lifting assembly comprises a lifting rod (801) and a lifting ring (802); the experimental platform (1) is provided with a lifting rod (801), the lifting rod (801) is sleeved with a lifting ring (802) located on the front side of the support plate (701), and a fixing bolt is screwed into the lifting ring (802).

7. A stem cell extraction and aspiration device according to claim 6, characterized in that: The lifting assembly also includes a rotating shaft (803); the front side of the support plate (701) is fixedly connected with the rotating shaft (803), and the rotating shaft (803) is movably connected to the lifting ring (802).

8. A stem cell extraction and aspiration device according to claim 6, characterized in that: The sliding assembly comprises a slide groove (901) and a slider (902); the slide groove (901) is provided on the experimental platform (1), the slider (902) is slidably connected in the slide groove (901), and the lifting column is fixedly connected to the slider (902).

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