Nucleic acid pollutant quantitative collection device applied to PCR (Polymerase Chain Reaction) laboratory

Abstract

The utility model provides a nucleic acid pollutant quantitative collection device applied to a PCR (Polymerase Chain Reaction) laboratory, which comprises a collection frame and a swab, the collection frame is used for limiting a sampling area, and the collection frame is folded or curled by using a foldable material, so that the collection frame can be switched between an open use state and a folded storage state; the swab comprises a rod part and a head part detachably connected with the rod part, the outer surface of at least one part of the head part is covered with a fiber material used for adhering a sample, a through hole is formed in the head part in the axial direction of the head part, the rod part comprises a main body and a support, the support is fixedly connected with one end of the main body, and the support is fixedly connected with the other end of the main body. The support comprises a cross rod perpendicular to the axis of the body, and the cross rod is combined with the through hole to enable the head to be rotatably connected to the support. According to the utility model, pollutants in unit area can be uniformly and comprehensively collected, the collection frame can be folded or curled into a smaller storage unit, the operation is simple and convenient, and the space is saved.

Description

Technical Field

[0001] The utility model relates to the technical field of sampling instruments, in particular to a nucleic acid pollutant quantitative collection device used in a PCR laboratory. Background Art

[0002] The PCR (Polymerase Chain Reaction) laboratory, also known as the gene amplification laboratory, can amplify specific DNA fragments through real-time fluorescence PCR technology and detect the virus content in the patient's body through a DNA gene tracking system.

[0003] PCR laboratory contamination is a common problem in PCR experiments. Due to the high sensitivity of PCR experiments, even trace amounts of contamination can have a significant impact on the test results, especially for nucleic acid detection experiments. Once contamination occurs, the first task is to identify the source of contamination and then remove the laboratory contamination in a targeted manner.

[0004] The current method of finding pollution sources is to use a cotton swab to wipe the surface of the suspected pollutant to collect a sample, elute it with distilled water, and then test the eluted liquid.

[0005] The problem with the above scheme is that since the sampling area is not defined, it is difficult to quantitatively calculate the pollutant value per unit area to determine the subsequent sewage discharge plan. In addition, when using cotton swabs to sample in a large sampling area, the operation is more laborious and it is difficult to achieve uniform and comprehensive sampling. Utility Model Content

[0006] The purpose of the utility model is to overcome the shortcomings of the above-mentioned prior art and provide a nucleic acid contaminant quantitative collection device for use in PCR laboratories that is easy to operate, convenient to store, and capable of achieving quantitative sampling.

[0007] In order to achieve the above-mentioned purpose, the nucleic acid contaminant quantitative collection device of the utility model is as follows:

[0008] The nucleic acid contaminant quantitative collection device has the following main features: it comprises a collection frame and a swab, wherein the collection frame is used to define a sampling area, and the collection frame is folded or curled using a foldable material, so that the collection frame can be switched between an open use state and a folded storage state;

[0009] The swab includes a rod and a head detachably connected to the rod, the outer surface of at least a portion of the head is covered with fiber material to adhere to the pollutant sample, the head is provided with a through hole along its axial direction, the rod includes a main body and a bracket, the bracket is fixedly connected to one end of the main body, the bracket includes a cross bar perpendicular to the axis of the main body, the cross bar is combined with the through hole to rotatably connect the head to the bracket.

[0010] The above-mentioned nucleic acid contaminant quantitative collection device, wherein the bracket also includes a limit rod, the first end of the cross rod is directly or indirectly connected to the main body, the second end of the cross rod is connected to the limit rod, the limit rod is used to limit the head from detaching from the cross rod, and the angle between the limit rod and the cross rod is greater than 0 degree and less than 180 degrees.

[0011] The above-mentioned nucleic acid contaminant quantitative collection device, wherein the bracket also includes a connecting rod, the first end of the connecting rod is connected to the main body, the second end of the connecting rod is connected to the cross rod, and the axes of the connecting rod, the cross rod and the limiting rod are in the same plane.

[0012] The above-mentioned nucleic acid contaminant quantitative collection device, wherein the device also includes a sampling container, the sampling container includes a bottle body and a cover body, the bottle body is provided with an opening, the cover body can be detachably covered on the opening, the bottle body stores distilled water, and the sampling container is used to seal the head after sampling and wash away the contaminants on the head.

[0013] In the above-mentioned nucleic acid contaminant quantitative collection device, a label is adhered to the outer wall of the sampling container, and the label is used to record the sampling information.

[0014] In the above-mentioned nucleic acid contaminant quantitative collection device, the cover body in the sampling container is hingedly connected to the bottle body.

[0015] In the above-mentioned nucleic acid contaminant quantitative collection device, the collection frame is rectangular.

[0016] In the above-mentioned nucleic acid contaminant quantitative collection device, the length and width of the collection frame are both set to 10 cm to facilitate quantitative calculation.

[0017] In the above-mentioned nucleic acid contaminant quantitative collection device, the head is cylindrical.

[0018] The beneficial effects of the nucleic acid pollutant quantitative collection device of the utility model are as follows:

[0019] 1. The nucleic acid pollutant quantitative collection device of the utility model realizes uniform and comprehensive quantitative collection of pollutants per unit area through a collection frame with a specific sampling area and a rolling sampling swab, and is simple to operate and easy to use;

[0020] 2. The collection frame in the nucleic acid contaminant quantitative collection device of the present invention can be folded or rolled into a smaller unit storage state to save storage space. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The concept, specific structure and technical effects of the present invention will be further described below in conjunction with the accompanying drawings to fully understand the purpose, characteristics and effects of the present invention.

[0022] Figure 1 It is a schematic diagram of the open state of the collection frame in one embodiment of the utility model;

[0023] Figure 2a This is a schematic diagram of the position of the collection frame in an embodiment of the utility model when it is folded for the first time;

[0024] Figure 2b This is a schematic diagram of the position of the collection frame in an embodiment of the utility model when it is folded for the second time;

[0025] Figure 2c This is a schematic diagram of the position of the collection frame in an embodiment of the utility model when it is folded for the third time;

[0026] Figure 3 It is a schematic diagram of the storage state of the collection frame in one embodiment of the utility model;

[0027] Figure 4 It is a schematic structural diagram of a swab in one embodiment of the utility model;

[0028] Figure 5 It is a structural schematic diagram of the rod portion of the swab in one embodiment of the utility model;

[0029] Figure 6 is a three-dimensional diagram of the head of a swab in one embodiment of the utility model;

[0030] Figure 7 It is a structural schematic diagram of a sampling container in one embodiment of the utility model.

[0031] Reference numerals

[0032] 1- Collection frame;

[0033] 2-swab; 21-rod; 211-main body; 212-bracket; 2121-connecting rod; 2122-cross rod; 2123-limiting rod; 22-head; 221-through hole;

[0034] 3-sampling container; 31-bottle body; 311-opening; 32-cover body. DETAILED DESCRIPTION

[0035] In order to make the technical means, creative features, objectives and effects of the utility model easier to understand, the utility model is further described below with reference to specific diagrams. However, the utility model is not limited to the following implementation cases.

[0036] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification so that people familiar with this technology can understand and read them. They are not used to limit the conditions under which the present utility model can be implemented, so they have no substantive technical significance. Any structural modification, change in proportional relationship or adjustment of size should still fall within the scope of the technical contents disclosed in the present utility model without affecting the effects and purposes that can be achieved by the present utility model.

[0037] like Figures 1 to 7 As shown, the utility model discloses a nucleic acid contaminant quantitative collection device applied to a PCR laboratory, comprising a collection frame 1, a swab 2 and a sampling container 3.

[0038] like Figure 1 As shown, the collection frame 1 is used to limit the sampling area. The collection frame 1 is rectangular, and its length and width are both 10 cm, so as to subsequently quantitatively calculate the number of nucleic acid contaminants per unit area. The collection frame 1 uses foldable materials to be folded or curled so as to switch between an open use state and a folded storage state. Figure 2a , Figure 2b , Figure 2c and Figure 3 One of the smaller folding states and the folding process of the collection frame 1 is shown, and the dotted line position in the above figure is the folding position of the collection frame 1.

[0039] like Figures 4 to 6 As shown, the swab 2 includes a stem 21 and a detachably connected head 22 , the head 22 is cylindrical, at least a portion of the outer surface of the head 22 is covered with a fiber material for adhering the sample, and the head 22 is provided with a through hole 221 along its axial direction.

[0040] The rod portion 21 includes a main body 211 and a bracket 212, the bracket 212 is fixedly connected to one end of the main body 211, the bracket 212 includes a connecting rod 2121, a cross bar 2122 and a limiting rod 2123, the axes of the connecting rod 2121, the cross bar 2122 and the limiting rod 2123 are located on the same plane; the first end of the connecting rod 2121 is connected to the end of the main body 211, the second end of the connecting rod 2121 is connected to the cross bar 2122, the cross bar 2122 is perpendicular to the axis of the main body 211, the cross bar 2122 can be combined with the through hole 221 to rotatably connect the head 22 to the bracket 212; the first end of the cross bar 2122 is connected to the connecting rod 2121, the second end of the cross bar 2122 is connected to the limiting rod 2123, the limiting rod 2123 is used to limit the head 22 from being separated from the cross bar 2122, and the angle between the limiting rod 2123 and the cross bar is greater than 0 degree and less than 180 degrees.

[0041] like Figure 7As shown, the sampling container 3 includes a bottle body 31 and a cover body 32. The bottle body 31 is provided with an opening 311. The cover body 32 is detachably covered on the opening 311 of the bottle body 21. The cover body 32 is hingedly connected to the bottle body 31. Distilled water is stored in the bottle body 31 to seal the head 22 after sampling and wash away pollutants on the head 22. A label is adhered to the outer wall of the sampling container 3 to record sampling information.

[0042] It should be noted that the preferred specific embodiments of the utility model are described in detail above. It should be understood that ordinary technology in the field can make many modifications and changes based on the concept of the utility model without creative work. Therefore, any technical solution that can be obtained by a technician in the technical field based on the concept of the utility model through logical analysis, reasoning or limited experiments on the basis of the existing technology should be within the scope of protection determined by the claims.

[0043] In the description of the present invention, it should be noted that the terms "upper / lower end", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0044] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "setting / sleeving", "socketing", "connection", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. The structures, proportions, sizes, etc. illustrated in the drawings attached to this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions that can be implemented in the present utility model, so they have no technical substantive significance. Any structural modification, change in proportional relationship or adjustment of size should still fall within the scope of the technical content disclosed in the present utility model without affecting the effects and purposes that can be achieved by the present utility model.

Claims

1. A nucleic acid contaminant quantitative collection device used in a PCR laboratory, characterized in that: The swab comprises a collection frame and a swab, wherein the collection frame is used to define a sampling area, and the collection frame is folded or curled using a foldable material so that the collection frame can be converted between an open use state and a folded storage state; The swab includes a rod and a head detachably connected to the rod, the outer surface of at least a portion of the head is covered with fiber material, the head is provided with a through hole along its axial direction, the rod includes a main body and a bracket, the bracket is fixedly connected to one end of the main body, the bracket includes a cross bar perpendicular to the axis of the main body, the cross bar is combined with the through hole to rotatably connect the head to the bracket.

2. The nucleic acid contaminant quantitative collection device according to claim 1, characterized in that: The bracket also includes a limit rod, the first end of the cross rod is directly or indirectly connected to the main body, the second end of the cross rod is connected to the limit rod, the limit rod is used to limit the head from separating from the cross rod, and the angle between the limit rod and the cross rod is greater than 0 degree and less than 180 degrees.

3. The nucleic acid contaminant quantitative collection device according to claim 2, characterized in that: The bracket further comprises a connecting rod, a first end of the connecting rod is connected to the main body, a second end of the connecting rod is connected to the cross rod, and axes of the connecting rod, the cross rod and the limiting rod are in the same plane.

4. The nucleic acid contaminant quantitative collection device according to claim 1, characterized in that: The device also includes a sampling container, which includes a bottle body and a cover body. The bottle body is provided with an opening, and the cover body is detachably covered on the opening. Distilled water is stored in the bottle body. The sampling container is used to seal the head after sampling and wash away pollutants on the head.

5. The nucleic acid contaminant quantitative collection device according to claim 4, characterized in that: A label is adhered to the outer wall of the sampling container, and the label is used to record sampling information.

6. The nucleic acid contaminant quantitative collection device according to claim 4, characterized in that: The cover body in the sampling container is hingedly connected to the bottle body.

7. The nucleic acid contaminant quantitative collection device according to claim 1, characterized in that: The acquisition frame is a rectangle.

8. The nucleic acid contaminant quantitative collection device according to claim 7, characterized in that: The length and width of the acquisition frame are both set to 10 cm to facilitate quantitative calculation.

9. The nucleic acid contaminant quantitative collection device according to claim 1, characterized in that: The head is cylindrical.

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