Biological material micro stretching table combined with atomic force microscope

Abstract

The utility model provides a micro biological material stretching table combined with an atomic force microscope, relates to the technical field of precision mechanical equipment, and aims to solve the technical problem that a sample is suspended when a biological material is stretched by a stretching table. The device comprises a base, and a through hole is formed in the base; the first clamp is arranged at one end of the upper surface of the base; the second clamp is arranged at the other end of the base; the movable sliding table is arranged on the base, and the second clamp is arranged on the movable sliding table and can move on the movable sliding table; the clamping surface of the first clamp is higher than the clamping surface of the second clamp; when the sample is clamped, the sample inclines towards one end of the second clamp relative to the surface of the base; the loading plate is arranged in the first clamp, and one end of the loading plate extends towards the sample clamping area. The stretching table disclosed by the utility model is convenient for fixing a biological sample and greatly prevents the sample from being suspended in the stretching process. The sample fixing method is easy to keep the relative flatness of the sample, so that the observation of the atomic force microscope is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of precision mechanical equipment, in particular to a biomaterial micro-stretching platform used in conjunction with an atomic force microscope. Background Art

[0002] Tensile testing is a common method for studying and testing the mechanical properties of materials. The technical principle is to apply a certain mechanical tensile force to the sample and measure the deformation of the sample during the extension process. Atomic force microscopy can observe the surface structure and mechanical properties of the sample at the nanoscale. Combining tensile testing with atomic force microscopy observations can observe in real time the changes in the nanostructure and mechanical properties of biomaterials when they are subjected to controllable tensile force or undergo a certain deformation. As an important means of observing the mechanical properties of materials and their molecular structure, the existing stretching table technology has been developed from research in the field of traditional materials and is generally suitable for relatively hard and regular-shaped materials. For example, patent publication number CN107290207A discloses a material testing device based on an atomic force microscope and an electrodynamic stretching machine, including a base, an atomic force microscope, and an electrodynamic stretching machine. The atomic force microscope is fixedly arranged on the electrodynamic stretching machine, and the electrodynamic stretching machine includes: a control host, a loading frame, two beams, and two clamps. The loading frame is provided with a lead screw, a synchronous belt, a servo motor, and a displacement sensor for detecting the displacement of the beam and a load sensor for detecting the load on the beam; the two beams are arranged in parallel and cooperate with the lead screw thread, the two clamps are fixedly arranged on the two beams relative to each other, and the control host is connected to the servo motor, the displacement sensor, the load sensor and the atomic force microscope through a circuit.

[0003] However, biomaterials that are relatively soft and of varying sizes are significantly different from traditional materials in terms of fixation, the amount of force applied, and measurement accuracy. In particular, biomaterials may appear suspended in the air during the pulling process. Existing stretching table technology often cannot meet the testing needs of biomaterials, is difficult to operate, and has a low success rate. Utility Model Content

[0004] In view of the technical problem that the stretching platform may cause the sample to be suspended when stretching the biological material, the utility model proposes a biomaterial micro-stretching platform used in conjunction with an atomic force microscope. The stretching platform is convenient for fixing the biological sample, and greatly avoids the occurrence of the sample being suspended during the stretching process. The sample fixing method is easy to keep the sample relatively flat, so as to facilitate atomic force microscope observation and greatly improve the success rate of the experiment.

[0005] In order to achieve the above object, the technical solution of the utility model is implemented as follows:

[0006] A biomaterial micro-stretching platform used in conjunction with an atomic force microscope comprises a base, a through hole is provided on the base, and the area above the through hole is a sample holding area;

[0007] A first clamp is provided at one end of the upper surface of the base;

[0008] The second clamp is provided at the other end of the base and cooperates with the first clamp to hold the sample;

[0009] A movable slide is arranged on the base, and the second fixture is arranged on the movable slide and can move on the movable slide to adjust the distance between the second fixture and the first fixture;

[0010] The holding surface of the first clamp is higher than the holding surface of the second clamp, and when the sample is held, the sample is inclined relative to the base surface toward one end of the second clamp;

[0011] The loading plate is arranged in the first fixture, one end of the loading plate extends toward the sample holding area and is suspended above the through hole, and the suspended section of the loading plate supports the sample.

[0012] The surface of the base is provided with a groove, and the movable slide is arranged in the groove, so that the supporting surface in the first clamp is higher than the supporting surface in the second clamp.

[0013] The first clamp includes a first upper pressing block, a first lower pressing block and a limiting groove. The first lower pressing block is fixed on the base. The first upper pressing block is arranged in the limiting groove to form a supporting opening between the first lower pressing block and the first upper pressing block. The first upper pressing block and the first lower pressing block are connected by bolts, and the size of the supporting opening is adjusted by screwing the bolts.

[0014] The loading plate is a rectangular sheet. When in use, the sample is placed on the loading plate and then held together in the holding port of the first fixture.

[0015] The second clamp includes a second upper pressing block and a second lower pressing block, the second lower pressing block is fixed on the movable slide, a holding opening is formed between the second upper pressing block and the second lower pressing block and connected by bolts, and the size of the holding opening is adjusted by screwing the bolts.

[0016] The movable slide comprises a slide rail and a slide frame. The slide frame is mounted on the slide rail. The slide frame is connected to a drafting device. The drafting device drives the slide frame to move along the slide rail.

[0017] The drafting device includes a baffle and an adjusting knob. The baffle is fixed on one end of the base close to the movable slide. The adjusting knob passes through the baffle and is connected to the slide frame. The adjusting knob and the baffle are threadedly matched. Rotating the adjusting knob drives the slide frame to move.

[0018] It also includes a tension sensor, which is arranged on the first clamp and is used to detect the load.

[0019] It also includes a displacement sensor, which is arranged on the movable slide and is used to detect the distance between the second clamp and the first clamp.

[0020] The base is provided with a plurality of positioning holes, which are matched with the atomic force microscope.

[0021] Beneficial effects of the utility model: The utility model sets the support surface lower than the base surface, supports the sample through the loading plate when supporting the biological sample, avoids the biological sample from being suspended in the stretched and straightened state, and the sample fixing method is easy to keep the sample relatively flat, so as to facilitate atomic force microscope observation and greatly improve the success rate of the experiment. The stretching table can also provide high control accuracy by adding tension sensors, displacement sensors, etc. In addition, in addition to biological samples, the stretching table disclosed in the present application is also suitable for various flexible and elastic samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 It is a structural schematic diagram of the micro stretching platform of the utility model.

[0024] Figure 2 It is a horizontal cross-sectional view of the micro-stretching platform of the utility model.

[0025] Figure 3 It is a vertical cross-sectional view of the micro-stretching platform of the utility model.

[0026] Figure 4 It is a structural schematic diagram of the micro-stretching platform of the utility model from another perspective.

[0027] In the figure, 1, base; 11, positioning hole; 12, through hole; 2, first clamp; 21, first upper pressure block; 22, first lower pressure block; 23, limit groove; 3, second clamp; 31, second upper pressure block; 32, second lower pressure block; 4, movable slide; 41, slide rail; 42, slide frame; 43, stretching device; 431, baffle; 432, adjusting knob. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] Example 1

[0030] A biomaterial micro-stretching stage used in conjunction with an atomic force microscope, such as Figure 1 As shown, it includes a base 1, on which a through hole 12 is provided, and the area above the through hole 12 is a sample holding area; a first clamp 2 is provided at one end of the upper surface of the base 1; a second clamp 3 is provided at the other end of the base 1, and cooperates with the first clamp 2 to hold the sample; there is a certain distance between the first clamp 2 and the second clamp 3, and the through hole 12 is located between the first clamp 2 and the second clamp 3; the structures of the first clamp 2 and the second clamp 3 can be the same or different, as long as they can play a role in fixing and tightening the sample.

[0031] The movable slide 4 is arranged on the base 1 and is spaced a certain distance from the first clamp 2. The second clamp 3 is arranged on the movable slide 4 and can be moved on the movable slide 4 and fixed at any position on the movable slide 4 to adjust the distance between the second clamp 2 and the first clamp 2. When in use, the second clamp 3 is first moved on the movable slide 4 to one end close to the first clamp 2 to hold the sample, and then the second clamp 3 is moved again to stretch the sample.

[0032] Furthermore, the holding surface of the first clamp 2 is higher than the holding surface of the second clamp 3, that is, there is a certain height difference between the first clamp 2 and the second clamp 3 on the base 1, and when the sample is held, the sample is inclined relative to the base surface toward one end of the second clamp 3;

[0033] The micro-stretching table also includes a loading plate, which is arranged in the first fixture 2, one end of which extends toward the sample holding area and is suspended above the through hole 12. The suspended section of the loading plate supports the sample, and the suspended distance is adjusted according to the size of the sample, the height difference between the first fixture 2 and the second fixture 3, etc. The loading plate can be an integral part with the first fixture 2, or it can be an independent detachable and replaceable component.

[0034] Example 2

[0035] A biomaterial micro-stretching stage used in conjunction with an atomic force microscope, such as Figure 1As shown, it includes a base 1, on which a through hole 12 is provided, and the area above the through hole 12 is the sample holding area; a first clamp 2 is provided at one end of the upper surface of the base 1; a second clamp 3 is provided at the other end of the base 1, and cooperates with the first clamp 2 to hold the sample; there is a certain distance between the first clamp 2 and the second clamp 3.

[0036] The movable slide 4 is arranged on the base 1 and is spaced a certain distance from the first clamp 2. The second clamp 3 is arranged on the movable slide 4 and can be moved on the movable slide 4 and fixed at any position on the movable slide 4 to adjust the distance between the second clamp 2 and the first clamp 2. When in use, the second clamp 3 is first moved on the movable slide 4 to one end close to the first clamp 2 to hold the sample, and then the second clamp 3 is moved again to stretch the sample.

[0037] Furthermore, the holding surface of the first clamp 2 is higher than the holding surface of the second clamp 3, that is, there is a certain height difference between the first clamp 2 and the second clamp 3 on the base 1, and when the sample is held, the sample is inclined relative to the base surface toward one end of the second clamp 3;

[0038] Specifically, a groove is provided on the surface of the base 1 , and the movable slide 4 is arranged in the groove, so that the supporting surface in the first clamp 2 is higher than the supporting surface in the second clamp 3 .

[0039] The micro-stretching table also includes a loading plate, which is arranged in the first fixture 2, one end of which extends toward the sample holding area and is suspended above the through hole 12. The suspended section of the loading plate supports the sample, and the suspended distance is adjusted according to the size of the sample, the height difference between the first fixture 2 and the second fixture 3, etc. The loading plate can be an integral part with the first fixture 2, or it can be an independent detachable and replaceable component.

[0040] Specifically, the carrier plate is a rectangular sheet, such as a glass slide. When in use, the sample is placed on the carrier plate and then clamped in the clamping opening of the first clamp 2. At this time, one end of the carrier plate is suspended on the through hole 12, and then the other end of the stretched sample is clamped on the second clamp 3. Since the second clamp 3 is lower than the first clamp 2, the sample will be tightly attached to the carrier plate when it is tightened.

[0041] Example 3

[0042] A biomaterial micro-stretching stage used in conjunction with an atomic force microscope, such as Figure 1-4As shown, it includes a base 1, on which a through hole 12 is provided, and the area above the through hole 12 is a sample holding area; a first fixture 2, which is provided at one end of the upper surface of the base 1; a second fixture 3, which is provided at the other end of the base 1, and cooperates with the first fixture 2 to hold the sample; a movable slide 4, which is provided on the base 1, and the second fixture 3 is provided on the movable slide 4, and can move on the movable slide 4 to adjust the distance between the first fixture 2; the holding surface of the first fixture 2 is higher than the holding surface of the second fixture 3, and when holding the sample, the sample is inclined toward one end of the second fixture 3 relative to the base surface; a loading plate, which is provided in the first fixture 2, and one end of the loading plate extends to the sample holding area, and is suspended above the through hole 12, and the suspended section of the loading plate supports the sample. The surface of the base 1 is provided with a groove, and the movable slide 4 is provided in the groove, so that the holding surface in the first fixture 2 is higher than the holding surface in the second fixture 3.

[0043] Specifically, the first clamp 2 includes a first upper pressing block 21, a first lower pressing block 22 and a limiting groove 23. The first lower pressing block 22 is fixed on the base 1. The first upper pressing block 21 is arranged in the limiting groove 23 to form a holding opening between the first lower pressing block 22. The limiting groove 23 is open on at least one side of the holding sample to avoid blocking the sample. The first upper pressing block 21 and the first lower pressing block 22 are connected by bolts, and the size of the holding opening is adjusted by screwing the bolts. The second clamp 3 includes a second upper pressing block 31 and a second lower pressing block 32. The second lower pressing block 32 is fixed on the movable slide 4. A holding opening is formed between the second upper pressing block 31 and the second lower pressing block 32 and connected by bolts. The size of the holding opening is adjusted by screwing the bolts. The first clamp 2 and the second clamp 3 have the same holding principle but different sizes.

[0044] The movable slide 4 includes a slide rail 41 and a slide frame 42. The slide frame 42 is arranged on the slide rail 41. The slide rail 41 has two parallel rails. The slide frame 42 is provided with a slot matching the slide rail 41. The slide frame 42 is connected to a drafting device 43. The drafting device 43 drives the slide frame 42 to move along the slide rail 41.

[0045] Furthermore, the drafting device 43 includes a baffle 431 and an adjusting knob 432. The baffle 431 is fixed on one end of the base 1 close to the movable slide 4. The adjusting knob 432 passes through the baffle 431 and is connected to the slide 42. Rotating the adjusting knob 432 drives the slide 42 to move. For example, the adjusting knob 432 and the baffle 431 are threadedly matched, and the adjusting knob 432 is movably connected to the slide 42. The adjusting knob 432 can rotate at the connection part. When the adjusting knob 432 is rotated, the rotation adjustment position changes, driving the slide 42 to move; or, the adjusting knob 432 is threadedly connected to the slide 42, and the adjusting knob 432 can rotate on the baffle 431. When the adjusting knob 432 is rotated, the rotation adjustment position remains unchanged, and only the slide 42 moves.

[0046] In order to realize automatic control and monitor the force and deformation in real time, the stretching table also includes a tension sensor, which is arranged on the first clamp 2 to detect the load. It also includes a displacement sensor (force < 0.5N, stretching distance 6-8mm, height < 15mm), which is arranged on the movable slide 4 to detect the distance between the second clamp 3 and the first clamp 2. In addition, the base 1 is provided with a plurality of positioning holes 11, which can cooperate with the protrusions on the atomic force microscope. Different positioning holes 1 are selected for fixing according to the size and position of the sample, which broadens the application range and improves the observation accuracy.

[0047] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A biomaterial micro-stretching stage used in conjunction with an atomic force microscope, characterized in that: The device comprises a base (1), wherein a through hole (12) is provided on the base (1), and the area above the through hole (12) is a sample holding area; A first clamp (2) is disposed at one end of the upper surface of the base (1); The second clamp (3) is arranged at the other end of the base (1) and cooperates with the first clamp (2) to hold the sample; A movable slide (4) is arranged on the base (1), and the second clamp (3) is arranged on the movable slide (4) and can be moved on the movable slide (4) to adjust the distance between the second clamp (3) and the first clamp (2); The holding surface of the first clamp (2) is higher than the holding surface of the second clamp (3), and when the sample is held, the sample is inclined relative to the base surface toward one end of the second clamp (3); The loading plate is arranged in the first fixture (2), one end of the loading plate extends toward the sample holding area and is suspended above the through hole (12), and the suspended section of the loading plate supports the sample.

2. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 1, characterized in that: The surface of the base (1) is provided with a groove, and the movable slide (4) is arranged in the groove, so that the supporting surface in the first clamp (2) is higher than the supporting surface in the second clamp (3).

3. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 2, characterized in that: The first clamp (2) comprises a first upper pressing block (21), a first lower pressing block (22) and a limiting groove (23); the first lower pressing block (22) is fixed on the base (1); the first upper pressing block (21) is arranged in the limiting groove (23) to form a holding opening between the first upper pressing block (21) and the first lower pressing block (22); the first upper pressing block (21) and the first lower pressing block (22) are connected by bolts, and the size of the holding opening is adjusted by screwing the bolts.

4. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 3, characterized in that: The loading plate is a rectangular thin sheet. When in use, the sample is placed on the loading plate and then held together in the holding opening of the first clamp (2).

5. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 4, characterized in that: The second clamp (3) comprises a second upper pressing block (31) and a second lower pressing block (32); the second lower pressing block (32) is fixed on the movable slide (4); a holding opening is formed between the second upper pressing block (31) and the second lower pressing block (32) and are connected by bolts; the size of the holding opening is adjusted by screwing the bolts.

6. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 5, characterized in that: The movable slide (4) comprises a slide rail (41) and a slide frame (42); the slide frame (42) is arranged on the slide rail (41); the slide frame (42) is connected to a drafting device (43); and the drafting device (43) drives the slide frame (42) to move along the slide rail (41).

7. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 6, characterized in that: The drafting device (43) comprises a baffle (431) and an adjusting knob (432). The baffle (431) is fixed on one end of the base (1) close to the movable slide (4). The adjusting knob (432) passes through the baffle (431) and is connected to the slide frame (42). Rotating the adjusting knob (432) drives the slide frame (42) to move.

8. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to any one of claims 5 to 7, characterized in that: It also comprises a tension sensor which is arranged on the first clamp (2) and is used to detect the load applied.

9. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 8, characterized in that: It also comprises a displacement sensor, which is arranged on the movable slide (4) and is used to detect the distance between the second clamp (3) and the first clamp (2).

10. The biomaterial micro-stretching stage used in conjunction with an atomic force microscope according to claim 9, characterized in that: The base (1) is provided with a plurality of positioning holes (11).

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