Polypeptide solid-phase synthesis reactor

Abstract

The utility model provides a polypeptide solid-phase synthesis reactor. The polypeptide solid-phase synthesis reactor comprises a reaction vessel, a filter part and an extrusion part, a waste liquid outlet is formed in one end of the reaction vessel, a feeding opening matched with the extrusion part is formed in the other end of the reaction vessel, the extrusion part is detachably connected with the reaction vessel through the feeding opening and can extend into the reaction vessel, and the extrusion direction of the extrusion part is from the feeding opening to the waste liquid outlet; the filter part is placed in the reaction vessel close to one end of the waste liquid outlet, and the projection of the filter part at one end of the reaction vessel provided with the waste liquid outlet covers the waste liquid outlet, so that the reaction liquid is extruded by the extrusion part and discharged from the waste liquid outlet through the filter part. The polypeptide solid-phase synthesis reactor provided by the utility model can realize large-scale high-flux synthesis, can complete the synthesis of a plurality of polypeptides at one time, ensures smooth reaction and effective treatment of waste liquid as the reaction vessel, the filter part and the extrusion part work together in the whole reaction process, and is simple in structure, convenient to mount and dismount and lower in cost.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide synthesis, in particular to a polypeptide solid phase synthesis reactor. Background Art

[0002] Peptides are compounds formed by α-amino acids linked together by peptide bonds and are intermediate products of protein hydrolysis. Compounds formed by the dehydration condensation of two amino acid molecules are called dipeptides. Similarly, there are tripeptides, tetrapeptides, pentapeptides, etc. Compounds formed by the dehydration condensation of 10 to 100 amino acid molecules are usually called polypeptides. In the past, polypeptide synthesis was carried out in solution and was called liquid phase synthesis. Since Merrifield successfully developed the solid phase polypeptide synthesis method in 1963, after continuous improvement and perfection, the solid phase method has become a common technology in polypeptide and protein synthesis today, showing advantages that cannot be matched by the classical liquid phase synthesis method, thus greatly reducing the difficulty of product purification at each step.

[0003] Since the peptide solid phase reaction is a mixture of amino acid small molecule solution and solid phase carrier, it is different from the liquid phase reaction materials that are uniformly dispersed in the system, and the materials are often unevenly stirred. This phenomenon is particularly obvious in large-scale production. Due to uneven stirring, the local temperature is too high, resulting in side reactions.

[0004] Most peptide solid-phase synthesis reactors currently on the market are usually non-detachable, and if high-throughput synthesis is required, they will take up a large space, have low reaction efficiency, and have a complex structure and high cost. Utility Model Content

[0005] The utility model aims at the deficiencies in the prior art and provides a polypeptide solid phase synthesis reactor, the scheme is as follows:

[0006] A polypeptide solid phase synthesis reactor, comprising: a reaction vessel, a filter element and an extrusion element;

[0007] The reaction vessel is provided with a waste liquid outlet at one end and a feed inlet matching the extrusion piece at the other end. The extrusion piece is detachably connected to the reaction vessel through the feed inlet and can extend into the reaction vessel. The extrusion direction of the extrusion piece is from the feed inlet to the waste liquid outlet.

[0008] The filter element is placed inside the reaction vessel near one end of the waste liquid outlet, and the projection of the filter element at one end of the reaction vessel where the waste liquid outlet is opened covers the waste liquid outlet, so that the reaction liquid is squeezed by the extruder through the filter element and discharged from the waste liquid outlet.

[0009] In a specific embodiment, the extrusion member includes a piston, an extrusion column and a pressing portion, wherein one end of the extrusion column close to the reaction vessel is connected to the piston, and one end of the extrusion column away from the reaction vessel is connected to the pressing portion.

[0010] In a specific embodiment, a flexible layer is provided at one end of the pressing portion away from the pressing column.

[0011] In a specific embodiment, the side wall of the piston abuts against the inner wall of the reaction vessel.

[0012] In a specific embodiment, it further comprises a sealing member for sealing the waste liquid outlet, wherein the sealing member is detachably connected to the waste liquid outlet.

[0013] In a specific embodiment, the sealing member is provided with an internal thread, and the waste liquid outlet is provided with an external thread matching the internal thread, so as to enable the sealing member to be meshed and connected with the waste liquid outlet.

[0014] In a specific embodiment, a transparent portion is provided on the outer surface of the reaction vessel, and scale lines are provided on the transparent portion.

[0015] In a specific embodiment, it also includes a holding portion, which is arranged at the feed port and connected to the reaction vessel.

[0016] In a specific embodiment, the gripping portion is provided with an opening matching the extrusion piece, and the extrusion piece passes through the opening to extend into the interior of the reaction vessel.

[0017] In a specific embodiment, a curved portion matching the shape of a finger is provided on a side of the gripping portion close to the reaction vessel.

[0018] Beneficial effects: The polypeptide solid-phase synthesis reactor provided by the utility model can realize large-scale high-throughput synthesis and complete the synthesis of multiple polypeptides at one time. During the entire reaction process, the reaction vessel, filter element and extrusion element work together to ensure the smooth progress of the reaction and the effective treatment of waste liquid. It has a simple structure, convenient installation and disassembly, and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0021] Figure 2 This is a schematic diagram of the filter structure of the utility model;

[0022] Figure 3 This is a schematic diagram of the structure of the extrusion part of the utility model;

[0023] Figure 4 This is a schematic diagram of the seal structure of the utility model;

[0024] Figure 5 This is a schematic diagram of the transparent part structure of the utility model;

[0025] Figure 6 It is a schematic diagram of the structure of the gripping part of the utility model.

[0026] The figures are marked as follows: 1-reaction dish; 2-filter element; 3-extrusion element; 31-piston; 32-extrusion column; 33-pressing part; 331-flexible layer; 4-waste liquid outlet; 5-feed port; 6-seal; 7-transparent part; 8-scale line; 9-grip part; 10-internal thread; 11-external thread; 12-opening; 13-arc-shaped part. DETAILED DESCRIPTION

[0027] The concept, specific structure and technical effects of the present invention will be clearly and completely described below in combination with the embodiments and drawings to fully understand the purpose, features and effects of the present invention.

[0028] In the following, various embodiments of the present invention will be described more fully. The present invention may have various embodiments, and adjustments and changes may be made therein. However, it should be understood that there is no intention to limit the various embodiments of the present invention to the specific embodiments disclosed herein, but rather the present invention should be understood to cover all adjustments, equivalents and / or alternatives that fall within the spirit and scope of the various embodiments of the present invention.

[0029] Hereinafter, the terms "include" or "may include" used in various embodiments of the present invention indicate the presence of disclosed functions, operations or elements, and do not limit the addition of one or more functions, operations or elements. In addition, as used in various embodiments of the present invention, the terms "include", "have" and their cognates are intended only to indicate specific features, numbers, steps, operations, elements, components or combinations of the foregoing, and should not be understood as first excluding the presence of one or more other features, numbers, steps, operations, elements, components or combinations of the foregoing or the possibility of adding one or more features, numbers, steps, operations, elements, components or combinations of the foregoing.

[0030] In various embodiments of the present invention, the expression "or" or "at least one of A or / and B" includes any combination or all combinations of the words listed at the same time. For example, the expression "A or B" or "at least one of A or / and B" may include A, may include B, or may include both A and B.

[0031] The expressions (such as "first", "second", etc.) used in the various embodiments of the present invention may modify the various components in the various embodiments, but may not limit the corresponding components. For example, the above expressions do not limit the order and / or importance of the elements. The above expressions are only used for the purpose of distinguishing one element from other elements. For example, the first user device and the second user device indicate different user devices, although both are user devices. For example, without departing from the scope of the various embodiments of the present invention, the first element may be referred to as the second element, and similarly, the second element may also be referred to as the first element.

[0032] It should be noted that in the present invention, unless otherwise clearly specified and defined, the terms "installation", "connection", "fixation" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] In the present invention, those skilled in the art need to understand that the terms indicating orientation or positional relationship herein are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0034] The terms used in the various embodiments of the present invention are only used for the purpose of describing specific embodiments and are not intended to limit the various embodiments of the present invention. As used herein, the singular form is intended to also include the plural form, unless the context clearly indicates otherwise. Unless otherwise defined, all terms used herein (including technical terms and scientific terms) have the same meaning as the meanings commonly understood by ordinary technicians in the field of the various embodiments of the present invention. The terms (such as the terms defined in the dictionary used in general) will be interpreted as having the same meaning as the contextual meaning in the relevant technical field and will not be interpreted as having an idealized meaning or an overly formal meaning, unless clearly defined in the various embodiments of the present invention.

[0035] Embodiment 1

[0036] The polypeptide solid phase synthesis reactor provided in this embodiment can realize large-scale high-throughput synthesis and complete the synthesis of multiple polypeptides at one time. During the entire reaction process, the reaction vessel, the filter element and the extrusion element work together to ensure the smooth progress of the reaction and the effective treatment of the waste liquid. It has a simple structure, is easy to install and disassemble, and has a low cost.

[0037] A polypeptide solid phase synthesis reactor, comprising: a reaction vessel 1, a filter element 2 and an extrusion element 3;

[0038] A waste liquid outlet 4 is provided at one end of the reaction vessel 1, and a feed inlet 5 matching the extrusion piece 3 is provided at the other end. The extrusion piece 3 is detachably connected to the reaction vessel 1 through the feed inlet 5 and can extend into the reaction vessel 1. The extrusion direction of the extrusion piece 3 is from the feed inlet 5 to the waste liquid outlet 4.

[0039] The filter element 2 is placed inside the reaction vessel 1 near one end of the waste liquid outlet 4 , and the projection of the filter element 2 on the end of the reaction vessel 1 where the waste liquid outlet 4 is opened covers the waste liquid outlet 4 , so that the reaction liquid is squeezed by the squeezing element 3 through the filter element 2 and discharged from the waste liquid outlet 4 .

[0040] This embodiment provides a polypeptide solid phase synthesis reactor, as shown in the attached Figure 1 As shown, the reactor comprises a reaction vessel 1, a filter 2 and an extrusion 3. During the entire reaction process, the reaction vessel 1, the filter 2 and the extrusion 3 work together to ensure the smooth progress of the reaction and the effective treatment of the waste liquid. The design of the reaction vessel 1 is intended to achieve effective mixing of the reaction substances and control of the reaction process. The waste liquid outlet 4 and the feed port 5 provided therein help to conveniently introduce raw materials and discharge waste liquid, while the design of the extrusion 3 enables the reaction substances to be fully stirred and mixed during the reaction process, thereby promoting the progress of the reaction. The extrusion 3 can be detachably connected to the reaction vessel 1 through the feed port 5 and can extend into the interior of the reaction vessel 1. During use, the extrusion 3 squeezes the reaction liquid from the feed port 5 into the reaction vessel 1 for solid phase synthesis reaction, and the waste liquid after the reaction is squeezed by the extrusion 3 to the waste liquid outlet 4 for discharge.

[0041] The filter element 2 is placed near one end of the waste liquid outlet 4 of the reaction vessel 1. Figure 2 As shown, the filter element 2, as one of the key components, adopts a flexible design with uniformly distributed pores, which effectively realizes the filtration and purification of the reaction liquid. Furthermore, the position of the filter element 2 in the reaction dish 1 makes its projection cover the waste liquid outlet 4. By placing the filter element 2 near the waste liquid outlet 4 and covering the projection of the waste liquid outlet 4, it can be ensured that the reaction liquid is filtered when it is squeezed out by the extrusion element 3, and impurities and solid residues are removed, thereby ensuring the purity and efficiency of the reaction.

[0042] In a specific embodiment, the extrusion member 3 includes a piston 31 , an extrusion column 32 and a pressing portion 33 . The extrusion column 32 is connected to the piston 31 at one end close to the reaction vessel 1 , and connected to the pressing portion 33 at another end away from the reaction vessel 1 .

[0043] In this embodiment, as shown in the attached Figure 3 As shown, the design of the extrusion piece 3 adopts a combination of a piston 31, an extrusion column 32 and a pressing portion 33. Among them, one end of the extrusion column 32 close to the reaction vessel 1 is connected to the piston 31, and the other end is connected to the pressing portion 33, thereby forming the structure of the entire extrusion piece 3.

[0044] The piston 31, as the core component of the extrusion member 3, can provide a stable driving force to promote the mixing and flow of the reaction substances during the reaction process. The extrusion column 32, as an extension of the piston 31, is responsible for transmitting the driving force to the reaction liquid inside the reaction vessel 1 to ensure that the reaction substances are fully stirred and mixed. On the other hand, the pressing portion 33 is connected to the end of the extrusion column 32 away from the reaction vessel 1, and its design helps to ensure the balance and stability during the extrusion process, so that the reaction substances can smoothly pass through the filter element 2 and be discharged from the waste liquid outlet 4.

[0045] Such a design can effectively ensure the stability and reliability of the extrusion member 3 during the reaction process, and provide solid support for the practical application of the peptide solid phase synthesis reactor. At the same time, such a design also facilitates the operation and control during the reaction process, and helps to achieve efficient peptide synthesis reaction and high-quality output of products.

[0046] In a specific embodiment, a flexible layer 331 is provided at one end of the pressing portion 33 away from the squeezing column 32. In this embodiment, a flexible layer 331 is specially provided at one end of the pressing portion 33 away from the squeezing column 32, providing a more reliable and convenient operation experience for the experimenter.

[0047] In a specific embodiment, the side wall of the piston 31 abuts against the inner wall of the reaction vessel 1 .

[0048] In this embodiment, the design of the piston 31 takes into account the close fit with the inner wall of the reaction vessel 1 to ensure effective sealing and stable pushing of the reaction substances during the extrusion process.

[0049] The side wall of the piston 31 is perfectly in contact with the inner wall of the reaction vessel 1, and this design ensures the airtightness and tightness between the two during extrusion. Through the complete contact between the piston 31 and the inner wall of the reaction vessel 1, it is possible to effectively prevent the leakage of the reaction substance or the entry of foreign impurities into the reaction system, thereby maintaining the purity of the reaction solution and the stability of the reaction conditions. This tight contact also helps to reduce the loss of energy and driving force, and improves the efficiency and reliability of the extrusion piece 3 during the reaction process.

[0050] In addition, the close fit between the side wall of the piston 31 and the inner wall of the reaction vessel 1 is also conducive to reducing friction resistance and energy loss, making the extrusion process smoother and more efficient. At the same time, this design also helps to avoid the problem of uneven mixing or obstruction of the flow of reaction substances caused by the gap between the piston 31 and the reaction vessel 1, ensuring the stability of the reaction process and the quality of the product.

[0051] In a specific embodiment, it further comprises a sealing member 6 for sealing the waste liquid outlet 4 , and the sealing member 6 is detachably connected to the waste liquid outlet 4 .

[0052] In the present embodiment, a seal 6 for sealing the waste liquid outlet 4 is also included. This design takes into account the prevention of leakage of the reaction liquid during the reaction and the need for effective sealing of the waste liquid outlet. The seal 6 is designed to be detachably connected to the waste liquid outlet 4, and this connection mode makes the installation and replacement of the seal 6 more convenient and flexible. Through the design of detachable connection, the operator can easily open or close the seal 6 to achieve effective sealing and control of the waste liquid outlet 4. This design not only facilitates the operation in the waste liquid treatment process, but also improves the maintainability and operability of the entire reaction system.

[0053] In a specific embodiment, the sealing member 6 is provided with an internal thread 10 , and the waste liquid outlet 4 is provided with an external thread 11 matching the internal thread 10 , so as to enable the sealing member 6 to be meshed and connected with the waste liquid outlet 4 .

[0054] In this embodiment, the design of the seal 6 takes into account the requirements for sealing and connection stability during the waste liquid treatment process. Therefore, the seal 6 is provided with an internal thread 10, and the waste liquid outlet 4 is provided with an external thread 11 matching the internal thread 10, which is used to enable the seal 6 to be meshed and connected with the waste liquid outlet 4. The setting of the internal thread 10 allows the seal 6 to easily match the external thread 11 during installation, and reliably screw into the waste liquid outlet 4, thereby forming a stable connection. This threaded connection design not only ensures the tight fit between the seal 6 and the waste liquid outlet 4, but also improves the stability and shock resistance of the connection, so that no accidental loosening or leakage occurs during the waste liquid discharge process. Through the precise matching of the internal thread 10 and the external thread 11, a reliable connection is formed between the seal 6 and the waste liquid outlet 4, effectively preventing the risk of waste liquid leakage, and ensuring the safety and controllability of the waste liquid discharge process. This design not only simplifies the installation and replacement process of the seal 6, but also improves the stability and service life of the entire system.

[0055] Optional, as attached Figure 4As shown, the seal 6 and the waste liquid outlet 4 can also be connected in a chimeric manner to achieve effective sealing and control of the waste liquid outlet 4. The seal 6 is provided with a groove matching the waste liquid outlet 4. During use, the seal 6 and the waste liquid outlet 4 are connected or not by plugging and unplugging. In actual applications, the connection method between the seal 6 and the waste liquid outlet 4 can also be selected according to actual needs.

[0056] In a specific embodiment, a transparent portion 7 is disposed on the outer surface of the reaction vessel 1 , and scale lines 8 are disposed on the transparent portion 7 .

[0057] In this embodiment, as shown in the attached Figure 5 As shown, in order to facilitate the operator to observe and monitor the reaction process, the designer deliberately sets a transparent part 7 on the outer surface of the reaction vessel 1. The transparent part 7 is made of transparent material, so that the operator can clearly see the liquid state and reaction situation inside the reaction vessel.

[0058] Furthermore, in order to better assist the operator in quantitative observation, scale lines 8 are also designed on the transparent portion 7. These scale lines 8 are accurately marked on the surface of the transparent portion 7 to indicate the volume or height change of the reaction liquid. Through these scale lines 8, the operator can easily grasp the amount of reaction liquid, reaction progress and liquid level change, which helps to accurately control experimental parameters and adjust experimental conditions.

[0059] The transparent portion 7 and the scale lines 8 enable the operator to intuitively understand the situation inside the reaction vessel without frequently opening the cover for inspection, thereby improving the convenience and efficiency of the operation. At the same time, through the design of the transparent portion 7 and the scale lines 8, the operator can promptly discover and handle possible abnormal situations, thereby ensuring the accuracy and safety of the experiment.

[0060] In practical applications, optionally, the reaction vessel 1 can be designed to be transparent as a whole according to the characteristics of the reaction liquid and scale lines 8 can be set on the outer surface of the reaction vessel 1 so that the operator can intuitively understand the situation inside the reaction vessel.

[0061] In a specific embodiment, it also includes a holding portion 9 , which is disposed at the feed port 5 and connected to the reaction vessel 1 .

[0062] In a specific embodiment, the gripping portion 9 is provided with an opening 12 matching the extrusion piece 3 , and the extrusion piece 3 passes through the opening 12 to extend into the interior of the reaction vessel 1 .

[0063] In a specific embodiment, a curved portion 13 matching the shape of a finger is provided on a side of the gripping portion 9 close to the reaction vessel 1 .

[0064] In this embodiment, as shown in the attached Figure 6As shown, in order to facilitate the extrusion operation of the experimenter, a gripping portion 9 is also provided at the feed port 5. The gripping portion 9 is connected to the reaction vessel 1, allowing the operator to easily control the feeding and reaction process, ensuring the convenience and efficiency of the operation.

[0065] The gripping portion 9 is provided with an opening 12 matching the extrusion piece 3, and the extrusion piece 3 can pass through the opening 12 and extend into the reaction vessel 1. This design allows the operator to precisely control the extrusion piece 3 through the gripping portion 9 and adjust the feeding speed to meet the needs under different experimental conditions.

[0066] Furthermore, a curved portion 13 matching the shape of a finger is provided on one side of the gripping portion 9 close to the reaction vessel 1. This design takes into account ergonomics and operational comfort, allowing the operator to grip and operate the gripping portion 9 more naturally, reducing hand fatigue and improving operational comfort and stability.

[0067] The polypeptide solid phase synthesis reactor provided by the utility model can realize large-scale high-throughput synthesis and complete the synthesis of multiple polypeptides (for example, 100 or more polypeptides) at one time. During the entire reaction process, the reaction vessel, the filter element and the extrusion element work together to ensure the smooth progress of the reaction and the effective treatment of the waste liquid. The structure is simple, the installation and disassembly is convenient, and the cost is low.

[0068] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A polypeptide solid phase synthesis reactor, characterized in that: include: Reactors, filters and extrusions; The reaction vessel is provided with a waste liquid outlet at one end and a feed inlet matching the extrusion piece at the other end. The extrusion piece is detachably connected to the reaction vessel through the feed inlet and can extend into the reaction vessel. The extrusion direction of the extrusion piece is from the feed inlet to the waste liquid outlet. The filter element is placed inside the reaction vessel near one end of the waste liquid outlet, and the projection of the filter element at one end of the reaction vessel where the waste liquid outlet is opened covers the waste liquid outlet, so that the reaction liquid is squeezed by the extruder through the filter element and discharged from the waste liquid outlet.

2. The polypeptide solid phase synthesis reactor according to claim 1, characterized in that: The extrusion member comprises a piston, an extrusion column and a pressing portion. The extrusion column is connected to the piston at one end close to the reaction vessel, and is connected to the pressing portion at one end away from the reaction vessel.

3. The polypeptide solid phase synthesis reactor according to claim 2, characterized in that: A flexible layer is provided at one end of the pressing portion away from the extrusion column.

4. The polypeptide solid phase synthesis reactor according to claim 2, characterized in that: The side wall of the piston abuts against the inner wall of the reaction vessel.

5. The polypeptide solid phase synthesis reactor according to claim 1, characterized in that: It also includes a sealing member for sealing the waste liquid outlet, and the sealing member is detachably connected to the waste liquid outlet.

6. The polypeptide solid phase synthesis reactor according to claim 5, characterized in that: The sealing member is provided with an internal thread, and the waste liquid outlet is provided with an external thread matching the internal thread, so as to enable the sealing member to be meshed and connected with the waste liquid outlet.

7. The polypeptide solid phase synthesis reactor according to claim 1, characterized in that: The outer surface of the reaction vessel is provided with a transparent portion, and scale lines are provided on the transparent portion.

8. The polypeptide solid phase synthesis reactor according to claim 1, characterized in that: It also includes a holding portion, which is arranged at the feed inlet and connected to the reaction vessel.

9. The polypeptide solid phase synthesis reactor according to claim 8, characterized in that: The gripping portion is provided with an opening matching the extrusion piece, and the extrusion piece penetrates through the opening to extend into the interior of the reaction vessel.

10. The polypeptide solid phase synthesis reactor according to claim 9, characterized in that: A curved portion matching the shape of a finger is arranged on one side of the gripping portion close to the reaction vessel.

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