Sorting and filtering device for tumor cell clusters
By designing rotating components and sorting boxes, using filters and collection boxes of different sizes to distinguish areas, the problem of cell mass not being effectively sorted in the prior art is solved, and efficient sorting of cell mass of different sizes and types is achieved and collect.
Patent Information
- Application Number
- CN202420783519.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-16
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2034-04-16
AI Technical Summary
During the filtration process of existing tumor cell cluster sorting and filtration devices, cell clusters can only pass through the bottom of the filter, resulting in accumulation of other parts, and it is impossible to effectively sort cell clusters of different sizes and types.
A device including a base plate, mounting plate, rotating assembly, sorting box, filter mesh and collection box was designed. The pulley and belt are driven by rotating the motor to realize the rotation of the sorting box, so that the cell mass is on filter mesh of different sizes. Sorting is performed and regions are distinguished by partitions and collection cassettes to ensure that cell clusters of different sizes and types can be filtered and collected separately.
Effective sorting and collection of cell clusters of different sizes and types is achieved, avoiding the accumulation and mixing of cell clusters during filtration, and improving sorting efficiency and accuracy.
Smart Images
Figure CN222226354U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of sorting and filtering, in particular to a sorting and filtering device for tumor cell clusters. Background Art
[0002] The cell substance is the tumor. Tumor tissue is composed of two parts: substance and stroma. Tumor substance is tumor cells, which are the main components of the tumor and have tissue origin specificity. It determines the biological characteristics of the tumor and the particularity of each tumor. When analyzing tumor cell clusters, they need to be sorted and filtered according to their size.
[0003] According to the announcement number (CN216946981), a tumor cell cluster sorting and filtering device is proposed, which includes an outer layer cylinder and a middle layer cylinder, which are detachably arranged inside the outer layer cylinder, a middle layer operating piece is arranged on the top of the outer side surface of the middle layer cylinder, a middle layer locking piece is arranged on the side of the middle layer operating piece close to the outer layer cylinder, an outer layer locking piece is arranged on the inner side surface of the outer layer cylinder at a position corresponding to the middle layer locking piece, and a locking piece is arranged on the operating piece, so that the middle layer cylinder and the outer layer cylinder can be stably locked, and the operating piece is used to realize the unlocking and removal between the middle layer cylinder and the outer layer cylinder, so that the operation of removing and replacing the filter becomes simple and fast.
[0004] However, when tumor cell clusters are sorted, although a filter is required for sorting, when the filter is used to sort and filter the tumor cell clusters in the above patent, the tumor cell clusters rely on their own gravity to pass through the mesh of the filter for filtration. In this filtering method, when the cell clusters contact the filter, only the bottom side of the cell clusters may pass through the filter, while the other parts cannot be sorted by the filter, resulting in the remaining cell clusters being accumulated on the surface of the filter. Therefore, a tumor cell cluster sorting and filtering device is proposed to solve the above problem. Utility Model Content
[0005] In view of the deficiencies of the prior art, the utility model provides a tumor cell cluster sorting and filtering device, which is used to solve the problems raised in the above background technology.
[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0007] A tumor cell cluster sorting and filtering device comprises a bottom plate, two mounting plates are fixedly mounted on the top side of the bottom plate, a rotating assembly is arranged on one side of one mounting plate, a sorting box is arranged between the two mounting plates, sliding grooves are provided on the inner walls on both sides of the sorting box, sliders are slidably mounted in the two sliding grooves, a same mounting frame is fixedly mounted on the side where the two sliders are close to each other, a partition is fixedly mounted on the inner wall of the mounting frame, the mounting frame is divided into two areas by the partition, a first filter screen and a second filter screen are fixedly mounted on the two areas respectively, the filtering holes provided by the first filter screen are smaller than the filtering holes provided by the second filter screen, sealing assemblies are arranged on the top layer and bottom side of the sorting box, an auxiliary rotating assembly is arranged on one side of the other mounting plate, and a classification collection assembly is arranged below the sorting box.
[0008] Preferably, the rotating assembly includes a mounting plate with a circular hole on one side, a movable column is arranged in the circular hole, one end of the movable column is fixedly connected to one side of the sorting box, the other end of the movable column is fixedly sleeved with a first pulley, a top plate is fixedly installed on the top side of a mounting plate, a rotating motor is fixedly installed on one side of the top plate, the output end of the rotating motor rotates through the top plate and is fixedly installed with a rotating rod, a second pulley is fixedly sleeved on the outer side of the rotating rod, and the same belt is arranged on the outer sides of the second pulley and the first pulley.
[0009] Preferably, the auxiliary rotating assembly includes a circular shaft fixedly mounted on one side of a mounting plate, a connecting plate fixedly connected to the other side of the circular shaft, a fixing column fixedly connected to the other side of the connecting plate, and the other end of the fixing column fixedly connected to one side of the sorting box.
[0010] Preferably, the sealing assembly includes discharge ports fixedly mounted on the top and bottom sides of the sorting box, the two discharge ports are both connected to the sorting box, and sealing pads are sealed and inserted in the two discharge ports.
[0011] Preferably, the classification collection assembly includes a collection box placed on the top side of the bottom plate, a fixing plate is fixedly installed on the inner wall of the collection box, the collection box is divided into two areas by the fixing plate, and the collection box is located below the sorting box.
[0012] Preferably, two inclined blocks are fixedly mounted on the top inner wall and the bottom inner wall of the sorting box, and the two groups of inclined blocks are symmetrically arranged.
[0013] Preferably, two feed inlets are provided on one side of the installation frame, and the two feed inlets are respectively connected to two areas separated by a partition.
[0014] Compared with the prior art, the utility model has the following beneficial effects: when it is necessary to sort and filter the cell clusters, the tumor cell cluster sorting and filtering device can divide the installation frame into two areas through the provided partition, so as to obtain cell clusters of different sizes, and the second pulley is driven to rotate by the rotating motor. When the second pulley drives the belt to rotate, the first pulley can synchronously drive the movable column to rotate, and because a circular shaft is provided on one side of the other mounting plate, the sorting box can rotate synchronously under the action of the circular shaft, and then the cell clusters in the two areas can respectively hit the first filter screen and the second filter screen, and because the filter holes of the first filter screen and the second filter screen respectively installed in the two areas are of different sizes, two groups of cell clusters of different sizes can be sorted out, and two groups of cell clusters of different types can also be sorted and filtered at the same time.
[0015] The collection box is divided into two areas by the fixed plate, which can place two groups of cell clusters of different sizes respectively to avoid mixing. The two inclined blocks can prevent the cell clusters from piling up at the corners of the sorting box during unloading, and can play a guiding role. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a three-dimensional schematic diagram of the structure of the utility model;
[0017] Figure 2 This is a schematic diagram of the internal parts of the sorting box structure of the utility model;
[0018] Figure 3 This is a schematic diagram of some parts of the belt structure of the utility model;
[0019] Figure 4 It is a schematic diagram of some parts of the circular shaft structure of the utility model.
[0020] In the figure: 1. bottom plate; 2. mounting plate; 3. round shaft; 4. connecting plate; 5. fixed column; 6. movable column; 7. sorting box; 8. first pulley; 9. top plate; 10. rotating motor; 11. rotating rod; 12. second pulley; 13. belt; 14. discharge port; 15. sealing gasket; 16. inclined block; 17. slide groove; 18. slider; 19. mounting frame; 20. partition; 21. first filter screen; 22. second filter screen; 23. feed port; 24. collecting box; 25. fixed plate. DETAILED DESCRIPTION
[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0022] Reference Figure 1-4 , a tumor cell cluster sorting and filtering device comprises a bottom plate 1, two mounting plates 2 are fixedly installed on the top side of the bottom plate 1, a rotating assembly is arranged on one side of one mounting plate 2, a sorting box 7 is arranged between the two mounting plates 2, both sides of the inner wall of the sorting box 7 are provided with slide grooves 17, two slide grooves 17 are slidably installed with sliders 18, a same mounting frame 19 is fixedly installed on the side where the two sliders 18 are close to each other, a partition plate 20 is fixedly installed on the inner wall of the mounting frame 19, the mounting frame 19 is divided into two areas by the partition plate 20, a first filter screen 21 and a second filter screen 22 are fixedly installed in the two areas, the filter holes provided in the first filter screen 21 are smaller than the filter holes provided in the second filter screen 22, a sealing assembly is arranged on the top and bottom sides of the sorting box 7, one side of the other mounting plate 2 is provided with a sealing assembly, An auxiliary rotating component is arranged on the side, and a classification and collection component is arranged at the bottom of the sorting box 7. By placing the tumor cell clusters into the installation frame 19 from the two feed ports 23 respectively, the placement position of the tumor cell clusters can be divided into two areas through the partition 20 arranged in the installation frame 19, so as to obtain tumor cell clusters of different sizes. When the sorting box 7 rotates, the tumor cell clusters in the two areas can hit the first filter screen 21 and the second filter screen 22 respectively, and because the filter holes of the first filter screen 21 and the second filter screen 22 respectively installed in the two areas are of different sizes, the tumor cell clusters can be sorted through the two filter holes of different sizes, so that two groups of tumor cell clusters of different sizes can be sorted out, and two groups of tumor cell clusters of different types can also be placed at the same time for sorting and filtering.
[0023] Specifically, the rotating assembly includes a mounting plate 2 with a circular hole on one side, a movable column 6 is arranged in the circular hole, one end of the movable column 6 is fixedly connected to one side of the sorting box 7, and the other end of the movable column 6 is fixedly sleeved with a first pulley 8, a top plate 9 is fixedly installed on the top side of a mounting plate 2, a rotating motor 10 is fixedly installed on one side of the top plate 9, the output end of the rotating motor 10 rotates through the top plate 9 and is fixedly installed with a rotating rod 11, a second pulley 12 is fixedly sleeved on the outer side of the rotating rod 11, and the second pulley 12 and the outer side of the first pulley 8 are provided with the same belt 13, the auxiliary rotating assembly includes a round shaft 3 fixedly installed on one side of a mounting plate 2, and the other end of the round shaft 3 is fixedly sleeved with a first pulley 8, and the second pulley 12 is fixedly sleeved with a second pulley 12. A connecting plate 4 is fixedly connected to one side, a fixing column 5 is fixedly connected to the other side of the connecting plate 4, and the other end of the fixing column 5 is fixedly connected to one side of the sorting box 7. The second pulley 12 is driven to rotate by starting the rotating motor 10. Because the second pulley 12 and the first pulley 8 are rotated by the same belt 13, when the second pulley 12 drives the belt 13 to rotate, the first pulley 8 can synchronously drive the movable column 6 to rotate. Because a circular shaft 3 is provided on one side of the other mounting plate 2, the sorting box 7 can rotate synchronously under the action of the circular shaft 3, so that the tumor cell clusters in the two areas can fully impact the first filter 21 and the second filter 22.
[0024] Specifically, the sealing component includes discharge ports 14 fixedly installed on the top and bottom sides of the sorting box 7, the two discharge ports 14 are connected to the sorting box 7, and sealing gaskets 15 are sealed and inserted in the two discharge ports 14. By sealing the two discharge ports 14 with sealing gaskets 15, it can be ensured that when the sorting box 7 rotates, the tumor cell clusters will not fall from the discharge ports 14. When unloading, it is only necessary to pull out the sealing gaskets 15 from the discharge ports 14.
[0025] Specifically, the classification collection component includes a collection box 24 placed on the top side of the bottom plate 1, a fixing plate 25 is fixedly installed on the inner wall of the collection box 24, and the collection box 24 is divided into two areas by the fixing plate 25. The collection box 24 is located below the sorting box 7, and two inclined blocks 16 are fixedly installed on the top inner wall and the bottom inner wall of the sorting box 7. The two sets of inclined blocks 16 are symmetrically arranged. Two feed ports 23 are opened on one side of the installation frame 19, and the two feed ports 23 are respectively connected to the two areas divided by the partition 20. The collecting box 24 is divided into two areas, which can be used to place two groups of tumor cell clusters of different sizes respectively to avoid mixing. The two inclined blocks 16 can prevent the tumor cell clusters from accumulating at the corners of the sorting box 7 during unloading, and can play a guiding role. The two feeding ports 23 can be used to simultaneously sort out two groups of tumor cell clusters of different sizes, and the two areas are separated by the partition 20 to avoid the two groups of tumor cell clusters from mixing together.
[0026] The tumor cell cluster sorting and filtering device is connected to a 220V mains power supply, and the main controller can be a conventional known device such as a computer for control.
[0027] During use: when it is necessary to sort the tumor cell clusters, the tumor cell clusters are placed into the installation frame 19 from the two feed ports 23 respectively. The partition 20 arranged in the installation frame 19 can divide the placement position of the tumor cell clusters into two areas, so as to obtain tumor cell clusters of different sizes. After the placement is completed, the second pulley 12 is driven to rotate by starting the rotating motor 10. Because the second pulley 12 and the first pulley 8 are rotated by the same belt 13, when the second pulley 12 drives the belt 13 to rotate, the first pulley 8 can synchronously drive the movable column 6 to rotate, and because a circular shaft 3 is arranged on one side of the other installation plate 2, the sorting box 7 can rotate synchronously under the action of the circular shaft 3. When the sorting box 7 rotates, the tumor cell clusters in the two areas can collide with the first filter screen 21 and the second filter screen 22 respectively, and because the filter holes of the first filter screen 21 and the second filter screen 22 respectively installed in the two areas are of different sizes, the tumor cell clusters can pass through the two sizes. Different filter holes are used for sorting, and two groups of tumor cell clusters of different sizes can be sorted out. Compared with the traditional sorting and filtering device, only a single tumor cell cluster can be sorted, and two groups of tumor cell clusters of different types cannot be sorted and filtered at the same time. After the sorting is completed, by aligning one discharge port 14 with the collection box 24, a sealing gasket 15 inserted in one discharge port 14 is pulled out, and then the tumor cell clusters sorted in one area will fall into the collection box 24, and then the sorting box 7 is turned over, and another sealing gasket 15 is pulled out, and the tumor cell clusters sorted in another area will also fall into the collection box 24, and the collection box 24 is divided into two areas by the fixing plate 25, and the two areas can respectively place tumor cell clusters of different sizes. When all the tumor cell clusters are sorted, the mounting frame 19 can be pulled out from the sorting box 7 by pulling the handle installed on one side of the mounting frame 19, and then the first filter screen 21 and the second filter screen 22 can be cleaned.
[0028] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0029] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A tumor cell cluster sorting and filtering device, comprising a bottom plate (1), characterized in that: Two mounting plates (2) are fixedly mounted on the top side of the bottom plate (1), a rotating assembly is arranged on one side of one mounting plate (2), a sorting box (7) is arranged between the two mounting plates (2), both inner walls of the sorting box (7) are provided with slide grooves (17), both slide grooves (17) are provided with sliders (18) slidably mounted, a same mounting frame (19) is fixedly mounted on the side where the two sliders (18) are close to each other, a partition (20) is fixedly mounted on the inner wall of the mounting frame (19), the mounting frame (19) is divided into two areas by the partition (20), a first filter screen (21) and a second filter screen (22) are fixedly mounted in the two areas respectively, the filter holes provided in the first filter screen (21) are smaller than the filter holes provided in the second filter screen (22), a sealing assembly is arranged on the top and bottom sides of the sorting box (7), an auxiliary rotating assembly is arranged on one side of the other mounting plate (2), and a classification collection assembly is arranged below the sorting box (7).
2. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: The rotating assembly comprises a mounting plate (2) having a circular hole on one side, a movable column (6) arranged in the circular hole, one end of the movable column (6) being fixedly connected to one side of a sorting box (7), the other end of the movable column (6) being fixedly sleeved with a first pulley (8), a top plate (9) being fixedly mounted on the top side of a mounting plate (2), a rotating motor (10) being fixedly mounted on one side of the top plate (9), the output end of the rotating motor (10) rotatingly passing through the top plate (9) and being fixedly mounted with a rotating rod (11), a second pulley (12) being fixedly sleeved on the outer side of the rotating rod (11), and the outer sides of the second pulley (12) and the first pulley (8) being provided with the same belt (13).
3. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: The auxiliary rotating assembly comprises a circular shaft (3) fixedly mounted on one side of a mounting plate (2); the other side of the circular shaft (3) is fixedly connected to a connecting plate (4); the other side of the connecting plate (4) is fixedly connected to a fixing column (5); the other end of the fixing column (5) is fixedly connected to one side of a sorting box (7).
4. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: The sealing assembly comprises discharge ports (14) fixedly mounted on the top and bottom sides of the sorting box (7), the two discharge ports (14) being connected to the sorting box (7), and sealing pads (15) being sealed and inserted in the two discharge ports (14).
5. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: The classification collection assembly comprises a collection box (24) placed on the top side of the bottom plate (1), a fixing plate (25) is fixedly mounted on the inner wall of the collection box (24), the collection box (24) is divided into two areas by the fixing plate (25), and the collection box (24) is located below the sorting box (7).
6. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: Two inclined blocks (16) are fixedly mounted on the top inner wall and the bottom inner wall of the sorting box (7), and the two groups of inclined blocks (16) are symmetrically arranged.
7. The tumor cell cluster separation and filtration device according to claim 1, characterized in that: Two feed inlets (23) are provided on one side of the installation frame (19), and the two feed inlets (23) are respectively connected to two areas separated by a partition plate (20).