Freezing pipe with filtering function

Abstract

The utility model discloses a freezing pipe with a filtering function. The freezing pipe comprises an outer sleeve, an upper pipe and a pipe cover, the bottom end of the upper tube is in threaded connection with the outer sleeve, and the upper end of the upper tube is in threaded connection with the tube cover; the upper pipe comprises a thick pipe, a funnel, a long thin pipe and a filter plug; the upper end of the funnel is fixedly connected with the thick pipe, and the lower end of the funnel is fixedly connected with the long thin pipe; the filter plug is positioned in the thick pipe and is naturally clamped above the funnel; the tube cover is in threaded connection with the thick tube; the outer sleeve is sleeved on the long and thin tube and is in threaded connection with the funnel; collected seminal plasma is placed in the thick pipe to be centrifuged, and centrifuged impurities are filtered through the filter plug and the funnel and are sealed through the pipe cover, so that liquid nitrogen can be effectively prevented from permeating into the upper pipe, and the phenomenon that the freezing pipe is exploded during unfreezing is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a freezing tube with a filtering function. Background Art

[0002] Sperm freezing is an assisted reproductive technology that aims to preserve male sperm for future use. It mainly refers to the use of cryogenic storage technology to preserve sperm in male semen in an ultra-low temperature environment for the purpose of treating infertility and preserving sperm. At present, the conventional steps for freezing sperm specimens are to centrifuge in a conical centrifuge tube, collect the precipitate and resuspend it, add an equal amount of cryoprotectant and mix it, and then divide it into cryovials for freezing; however, this method is not suitable for rare sperm specimens obtained from severe oligoasthenozoospermia and those requiring surgical sperm retrieval.

[0003] Existing rare sperm cryo-carriers, i.e. cryotubes, are divided into two categories, open and closed, depending on whether they are in direct contact with liquid nitrogen. In open carriers, such as cryotops, sperm is frozen by direct contact with liquid nitrogen, which may pose a risk of cross-contamination; in Cryopiece single sperm freezing technology (ultra-thin cryo-slide single sperm freezing technology), sperm with good morphology and activity are picked out one by one by a micromanipulator and placed on a special ultra-thin cryo-slide, and then placed in cryotubes for freezing. However, as the freezing time increases, liquid nitrogen will gradually enter the cryotube. When liquid nitrogen penetrates into the cryotube, it is easy for the cryotube to burst during thawing; in closed carriers, such as straws, the mouth is sealed after the sperm specimen is absorbed.

[0004] In freezing rare sperm, if conventional freezing technology is used, most of the sperm will be lost, and the number of sperm left will be very small; therefore, the most commonly used Cryopiece single sperm freezing technology is used to freeze rare sperm. Although this technology does not come into contact with liquid nitrogen and there is no risk of contamination, the tube mouth of this carrier is relatively small, the technical requirements are high, the operation is complicated, and it is not easy to mark the patient's detailed information on the tube wall, which makes it inconvenient for subsequent staff to distinguish the information. Utility Model Content

[0005] The utility model aims to provide a freezing tube with a filtering function which can filter impurities in seminal plasma or the broken seminiferous tubule tissue blocks after sperm extraction surgery, prevent liquid nitrogen from entering the tube, and is easy to operate, so as to solve the technical problem that the prior art cannot well filter impurities in seminal plasma and the broken seminiferous tubule tissue blocks after sperm extraction surgery and liquid nitrogen from entering the tube.

[0006] In order to solve the above technical problems, the solution adopted by the utility model is as follows:

[0007] A freezing tube with filtering function, comprising an outer sleeve, an upper tube and a tube cover; the bottom end of the upper tube is threadedly connected to the outer sleeve, and the upper end is threadedly connected to the tube cover; the upper tube comprises a thick tube, a funnel, a long thin tube and a filter plug; the upper end of the funnel is fixedly connected to the thick tube, and the lower end is fixedly connected to the long thin tube; the filter plug is located in the thick tube, above the funnel, and the filter plug is sheet-shaped; the filter plug is movably connected to the funnel to transfer a sperm specimen added with freezing liquid to the thick tube. During centrifugal operation, larger tissue fragments or impurities are blocked in the thick tube by the filter plug, and a small amount of slightly coarse tissue fragments will be collected in the funnel; the tube cover is threadedly connected to the thick tube; the outer sleeve is sleeved on the long thin tube and threadedly connected to the funnel.

[0008] Furthermore, the filter plug is provided with a handle; the handle is fixedly connected to the filter plug, and a black mark is provided on the upper end of the handle, so that the staff can quickly pick it up. The filter plug is taken out of the thick tube or placed in the thick tube by clamping the black mark part of the handle with tweezers.

[0009] Furthermore, the filter plug is fixed in position by the funnel. The filter plug is placed just above the funnel.

[0010] Furthermore, the thick tube is provided with an information marking area. The collected patient information is filled in the information marking area to mark the patient information, which is convenient for subsequent verification, recording and use by medical staff.

[0011] Furthermore, an anti-permeation ring is provided between the thick tube and the tube cover; the bottom end of the outer tube is hollowed out. The anti-permeation ring can further prevent the liquid nitrogen from penetrating into the thick tube through the tube cover, and the hollow bottom end of the outer tube facilitates the liquid nitrogen to directly contact the long thin tube during freezing to achieve rapid cooling and rapid rewarming during thawing. The working principle of the utility model is as follows:

[0012] When in use, add the specimen containing rare sperm into the sperm cryoprotectant and mix well, transfer the suspension to the thick tube of the upper tube, tighten the outer sleeve onto the funnel at the lower end of the thick tube, cover the tube cap, centrifuge the sperm, and collect the sperm in the long and thin tube. Larger tissue fragments or impurities are blocked in the thick tube by the filter plug, a small amount of slightly coarse tissue fragments will be collected in the funnel, and clean sperm-rich liquid will be concentrated in the long and thin tube. Open the tube cap, take out the filter plug through the handle, pour out the supernatant in the thick tube (above the filter plug), add embryo culture oil to fill the thick tube, and then screw the tube cap onto the thick tube to seal it. Fill in the patient's identity information in the information label area for future verification and backup. The bottom end of the outer sleeve is hollowed out, so that the long and thin tube can directly contact the liquid nitrogen for rapid freezing.

[0013] The beneficial effects of the utility model are as follows:

[0014] 1. The utility model places the collected sample containing rare sperm into a thick tube for centrifugation, and the impurities centrifuged are filtered through a filter plug and a funnel, and the tube is sealed by a tube cover, which can effectively prevent liquid nitrogen from penetrating into the upper tube and causing the freezing tube to burst during thawing.

[0015] 2. The design of the handle in the utility model can place the filter plug in the thick tube or take out the thick tube, which is convenient for taking out the filter plug after centrifugal filtration to remove impurities and obtain a cleaner sperm specimen.

[0016] 3. The utility model is provided with an information marking area. The collected patient information is filled in the information marking area to mark the patient information, which is convenient for the subsequent verification, recording and use by medical staff, and avoids the occurrence of wrong taking. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a front view structural schematic diagram of the utility model;

[0018] Figure 2 It is a front view cross-sectional structural schematic diagram of the utility model;

[0019] Figure 3 It is an explosion diagram of the utility model.

[0020] In the figure: 1. outer sleeve; 2. upper tube; 201. thick tube; 202. funnel; 203. long thin tube; 204. filter plug; 205. handle; 3. tube cover; 4. information marking area; 5. anti-permeation ring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present utility model, it should be noted that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0023] The following is a further detailed description of a freezing tube with filtering function of the utility model in conjunction with the accompanying drawings: Example

[0024] A freezing tube with a filtering function comprises an outer sleeve 1, an upper tube 2 and a tube cover 3; the bottom end of the upper tube 2 is threadedly connected to the outer sleeve 1, and the upper end is threadedly connected to the tube cover 3; the upper tube 2 comprises a thick tube 201, a funnel 202, a long thin tube 203 and a filter plug 204; the upper end of the funnel 202 is fixedly connected to the thick tube 201, and the lower end is fixedly connected to the long thin tube 203; the filter plug 204 is located in the thick tube 201, above the funnel 202; the tube cover 3 is threadedly connected to the thick tube 201; the outer sleeve 1 is sleeved on the long thin tube 203, and is threadedly connected to the funnel 202.

[0025] The working principle of this embodiment is as follows:

[0026] When in use, add the rare sperm sample to the sperm cryoprotectant and mix well, transfer 1000 μl of the suspension to the thick tube 201 of the upper tube 2, tighten the outer sleeve 1 onto the funnel 202 at the lower end of the thick tube 201, cover the tube cover 301, centrifuge at 500 g for 3 minutes, collect the sperm-rich liquid into the long and thin tube 203, and the larger tissue fragments or impurities are blocked by the filter plug 204 in the thick tube 201, and a small amount of slightly coarse tissue fragments will be collected in the funnel 202. Take out the filter plug 204, and remove the thick tube 201 (above the filter plug 204). ) is removed, embryo culture oil is added to fill the upper tube 2, and then the tube cover 3 is screwed into the thick tube 201 to seal it, and then transferred to liquid nitrogen for freezing; when thawing later, the device is taken out of the liquid nitrogen, put into 37°C water for water bath thawing, and after thawing, the outer sleeve 1 is removed from the funnel 202, the long thin tube 203 is taken out, the middle part of the long thin tube 203 is clamped with tweezers, and the long thin tube 203 is cut off from the bottom of the funnel 202 with sterile scissors, and the opening of the long thin tube 203 is placed in a dish containing sperm culture medium, so that the sperm-rich specimen naturally flows into the culture medium. Example

[0027] The difference from Example 1 is that the filter plug 204 is provided with a handle 205; the handle 205 is fixedly connected to the filter plug 204; and the filter plug 204 is fixed by the funnel 202. The medical staff clamps the handle 205 with sterile tweezers and puts the filter plug 204 into the thick tube 201 and above the funnel 202 to filter the centrifuged sperm. After the filtration is completed, the filter plug 204 is taken out of the thick tube 201 by clamping the handle 205 with sterile tweezers.

[0028] The working principle of this embodiment is the same as that of Embodiment 1. Example

[0029] The difference from Example 2 is that the thick tube 201 is provided with an information marking area 4; an anti-permeability ring 5 is provided between the thick tube 201 and the tube cover 3; the bottom end of the outer sleeve 1 is hollow; the anti-permeability ring 5 between the thick tube 201 and the tube cover 3 can further prevent liquid nitrogen from penetrating into the thick tube 201 through the tube cover 3, and the hollow bottom end of the outer sleeve 1 facilitates direct contact of liquid nitrogen with the long and thin tube 203 to freeze the sperm in the long and thin tube 203; after the tube cover 3 is covered, the collected patient information is filled in the information marking area 4 to mark the patient information, which is convenient for subsequent medical staff to verify, record and use.

[0030] The working principle of this embodiment is the same as that of Embodiment 2.

[0031] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A freezing tube with filtering function, characterized in that: The invention comprises an outer sleeve (1), an upper tube (2) and a tube cover (3); the lower end of the upper tube (2) is threadedly connected to the outer sleeve (1), and the upper end is threadedly connected to the tube cover (3); the upper tube (2) comprises a thick tube (201), a funnel (202), a long thin tube (203) and a filter plug (204); the upper end of the funnel (202) is fixedly connected to the thick tube (201), and the lower end is fixedly connected to the long thin tube (203); the filter plug (204) is located inside the thick tube (201) and above the funnel (202); the tube cover (3) is threadedly connected to the thick tube (201); the outer sleeve (1) is sleeved on the long thin tube (203) and threadedly connected to the funnel (202).

2. A freezing tube with filtering function according to claim 1, characterized in that: The filter plug (204) is provided with a handle (205); the handle (205) is fixedly connected to the filter plug (204).

3. A freezing tube with filtering function according to claim 2, characterized in that: The opening size of the funnel (202) is consistent with the size of the filter plug (204); the filter plug (204) is limited and fixed by the funnel (202).

4. The freezing tube with filtering function according to claim 1, characterized in that: The thick tube (201) is provided with an information marking area (4).

5. The freezing tube with filtering function according to claim 1, characterized in that: An anti-seepage ring (5) is provided between the thick tube (201) and the tube cover (3); and the bottom end of the outer sleeve (1) is hollowed out.

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